LOCUS       NZ_CP087661            11970 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01635 plasmid
            pDD01635-4, complete sequence.
ACCESSION   NZ_CP087661
VERSION     NZ_CP087661.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822720
            Assembly: GCF_023066785.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066785.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:21:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,042
            CDSs (total)                      :: 5,920
            Genes (coding)                    :: 5,727
            CDSs (with protein)               :: 5,727
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 193
            CDSs (without protein)            :: 193
            Pseudo Genes (ambiguous residues) :: 0 of 193
            Pseudo Genes (frameshifted)       :: 70 of 193
            Pseudo Genes (incomplete)         :: 136 of 193
            Pseudo Genes (internal stop)      :: 23 of 193
            Pseudo Genes (multiple problems)  :: 35 of 193
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087661.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01635"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01635-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(join(11261..11970,1..22))
                     /gene="mobC"
                     /locus_tag="LMH51_RS29960"
                     /old_locus_tag="LMH51_29960"
     CDS             complement(join(11261..11970,1..22))
                     /gene="mobC"
                     /locus_tag="LMH51_RS29960"
                     /old_locus_tag="LMH51_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(22..1986)
                     /locus_tag="LMH51_RS29965"
                     /old_locus_tag="LMH51_29965"
     CDS             complement(22..1986)
                     /locus_tag="LMH51_RS29965"
                     /old_locus_tag="LMH51_29965"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3470..3619)
                     /locus_tag="LMH51_RS29970"
                     /old_locus_tag="LMH51_29970"
     CDS             complement(3470..3619)
                     /locus_tag="LMH51_RS29970"
                     /old_locus_tag="LMH51_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3704..3961)
                     /locus_tag="LMH51_RS29975"
                     /old_locus_tag="LMH51_29975"
     CDS             complement(3704..3961)
                     /locus_tag="LMH51_RS29975"
                     /old_locus_tag="LMH51_29975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3971..5656)
                     /locus_tag="LMH51_RS29980"
                     /old_locus_tag="LMH51_29980"
     CDS             complement(3971..5656)
                     /locus_tag="LMH51_RS29980"
                     /old_locus_tag="LMH51_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(5761..7248)
                     /gene="ltrA"
                     /locus_tag="LMH51_RS29985"
                     /old_locus_tag="LMH51_29985"
     CDS             complement(5761..7248)
                     /gene="ltrA"
                     /locus_tag="LMH51_RS29985"
                     /old_locus_tag="LMH51_29985"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7893..8138
                     /locus_tag="LMH51_RS29990"
                     /old_locus_tag="LMH51_29990"
     CDS             7893..8138
                     /locus_tag="LMH51_RS29990"
                     /old_locus_tag="LMH51_29990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8412..8783
                     /gene="tolA"
                     /locus_tag="LMH51_RS29995"
                     /old_locus_tag="LMH51_29995"
     CDS             8412..8783
                     /gene="tolA"
                     /locus_tag="LMH51_RS29995"
                     /old_locus_tag="LMH51_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8780..9145
                     /locus_tag="LMH51_RS30000"
                     /old_locus_tag="LMH51_30000"
     CDS             8780..9145
                     /locus_tag="LMH51_RS30000"
                     /old_locus_tag="LMH51_30000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            9320..9598
                     /locus_tag="LMH51_RS30005"
                     /old_locus_tag="LMH51_30005"
     CDS             9320..9598
                     /locus_tag="LMH51_RS30005"
                     /old_locus_tag="LMH51_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            9586..9900
                     /locus_tag="LMH51_RS30010"
                     /old_locus_tag="LMH51_30010"
     CDS             9586..9900
                     /locus_tag="LMH51_RS30010"
                     /old_locus_tag="LMH51_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            10064..10492
                     /locus_tag="LMH51_RS30015"
                     /old_locus_tag="LMH51_30015"
     CDS             10064..10492
                     /locus_tag="LMH51_RS30015"
                     /old_locus_tag="LMH51_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            10518..10697
                     /locus_tag="LMH51_RS30020"
                     /old_locus_tag="LMH51_30020"
     CDS             10518..10697
                     /locus_tag="LMH51_RS30020"
                     /old_locus_tag="LMH51_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(10724..11254)
                     /locus_tag="LMH51_RS30025"
                     /old_locus_tag="LMH51_30025"
     CDS             complement(10724..11254)
                     /locus_tag="LMH51_RS30025"
                     /old_locus_tag="LMH51_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     BGC             1..10656
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2789..3367
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(11265..11968)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.043"
                     /coverage="97.0"
                     /evalue="3.82e-136"
ORIGIN
        1 cgttgtactg ctccagtgtc atcagtaaag ccccgcaaaa tcatcatccg cttttggtcc
       61 gccgccgtcg gccggaggca ggtactccgc cagcgggtcc gttttcgcca gcaccgggaa
      121 ggttttcagc gaaatttccc ggcgctcaac ccgcagcggg ctgctgaacg ccagccgcgc
      181 cacgcccagc ccggtcatca ccgcgaaccc gtcaggcagg tgttgcaggg tgttcacgtc
      241 aaacagcggc cgcgcctcct tctggatgaa gctgtcgccg ctgatgtgct ccacgttccc
      301 cgcctcccgg ctggcccggc ggcgctccac gtccacccgg atctccccgg tctggcccgc
      361 cgcccactgc gcggactcct ggctggccgc gcgatagaac cagcggatcg gcgtgttatc
      421 cagtaccgtg gttttcacaa aatcagcggg cagatcctgc ccgcactgac cgaagtcgcc
      481 cagcgactgg tgcgcgagca gcaggttgca gtcccggctg cgcagcgtcc ccagcgcgtt
      541 cagcacgtac ttcgacagca gatatttaat ctcatcgagc atgatgctcg cgtgcggcca
      601 cctccggaac tcatcccgcg caatgatgat ttgcgcgcag cgggcaaaca gcatcttctg
      661 cacccggatc accgcctcat cgtccatcga gccgatgacg tacaggcagc cgccgccgtt
      721 gattatcccc gccacatcga taccgctgtc cgtctgcagg acgctcaggt cggccacgtt
      781 ctccagcttg gtgatcagcc ccttcacgtc ctttttcagg gcctccggca acgcgtacgc
      841 cgccgccagc acctgctgaa tgtttgcccc ctgcgggaac tgctccgcaa tcagcttcgc
      901 cgctttctgc tcgctgatgc ggtagtggtc cgccacgtcg ccggtctcac tcaggttaaa
      961 cccggcaacc agcagattct tcagggcgta ctggtcgaga tcgcggaaca gattcagctg
     1021 cgccggtttc cccggccgca gatccagcag ggtgaagtcc gggcagtgcg cccggaagac
     1081 gtgcggcgcc cacgcgtccc ctttggggtc gaagtacacc acagcgtccc cgaaatcgcg
     1141 tacgcagcga atgagcgcgt tggtggccat cgcgctcttg ccgctcccgg gcaggccgag
     1201 gatctgaata ttggtcttgc gccaggtatc acgagggacc agcaccggct ggccctgctc
     1261 atccttaccc agaaacaccg cctgctgttt ccgggcctgc tccgtggaaa aatcggccag
     1321 cgcgttaacc ggtaccggct ccccgaaatg caggtggcgg acgtctgtca tttctccggt
     1381 ccgctggctg ctgcgccggc tctcctgatt cagtttcgcg ttcaccgcct cgtcagcggc
     1441 cgcaaaatga ccatcaagca gcaggaacgc cagcagccgc gccagaagga cgccggcgac
     1501 aagccctccg gcacaccagg ctatcccgaa caggttagcc ggctgcagca ggtaataatc
     1561 tgcgttcgca tagaggtccg ggcgcaggac ggtttctccc cagctctcga taaacacggc
     1621 gacaaccagc ccccccagaa gggccagacc ggcagcgccg gtcagccagt acgcgtagtt
     1681 ccagacatgt cgccgtgccc tgaccatccg cccgggtgaa tccagcgagg ccagcaccga
     1741 aaatggcaca gtccggttat tatcgcgctt atagcgatcg gcatactccc gccgcgcctt
     1801 atggctcagc cccacaaaac gcattcggcc ggcccagagt tcagccagac cgaaaaacag
     1861 gccgataacc agaaaaaaac cggccagact gtaatactcc cggtacagcc agctgaccca
     1921 cgggtaaatc tgccgggcca gctccgggat atgccactgc caggcaagcg aatccgtatt
     1981 aaacatgcaa tctctctcct tcagacgacg ggtttaccgg cgtgatgcgc agcccccgct
     2041 gcacaagccg cggacatgcc ccaaggcatg gcggcggcgg tcacgtccgt ggtgattgcg
     2101 ttcagctttc accgcggagg ccagcctctt tccgccccat gcggaaaagg cgcagccccg
     2161 gaggaacaac gagcacgccc cagcgtgtga gaggaaaagg cggcacccca gtgcggtctt
     2221 ttccggtgtt ccggcccccg tcagagttcg ccatagcgag cggctgacca cccgcaaaat
     2281 caggagcagc tttgctgctt cgtcaggtgc gtccagcacc gtggattttt gtgggtggtc
     2341 aggcgtgagc ctgacggggg ccgggtgaat ttctggcgtg gccggcgtcc agccggacag
     2401 agccagaaag aggggaaaag accgtgcgtc ggtggcgtcc agccagcgta agcaaggtct
     2461 tttcccctga agtcagccac gccccagcgg ggatgacttg catttctccg caaggagaaa
     2521 aaagcaacag agataggtta acgtaaaatg ttcagcgaaa aaccgacatg gttcacctat
     2581 cctgataatt gatcgtcagg gaatagaaag acttaatcag gggacaatat cccacatcag
     2641 cgcgtccggt gaccgtcttg caggtgccgg ggaaattgcg cctcctcgct cactcggtcg
     2701 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
     2761 ttccgtggat aagcagggga ctaacatgtg aggcaaaacg gcagggccgc gccggtggcg
     2821 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt tttcggtgca
     2881 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
     2941 ttaaagatcc ccaccgtttc cggtaggtcg ctccctcttg cgctctcctg ttccgaccct
     3001 gccgtttacc ggatacctgt ccgccgttct cccttgcggg aagtgtggcg ctttctcata
     3061 gctcacacac tggtatctcg gctcggtgta ggtcgttcgc tccaagctgg gctgtaagca
     3121 agaactcccc gttcagcccg actgctgcgc cttatccggt aactgttcac ttgagtccaa
     3181 cccgggaaag cacgcaaaac gccactggca gcagccattg gtaactggga gttcgcagag
     3241 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
     3301 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc
     3361 caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa
     3421 aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg
     3481 cgatccccgt cagttcagag gaggacgatg gtgccaccgt tccgccctga acatcccgga
     3541 tataatttgc ctgacatgcg gccagaagaa agccagcaac aatcaatata aaaagaaaaa
     3601 tagtctttac ctttttcata tactttccct gtatttaaag actccggcat tcatccctga
     3661 acaccggaag tcagtatttc aaatcatcct tgataatttt ttgttaccag ttaccgtccc
     3721 gataatcaaa agctatataa caattatcca catcaggttt tatttgattt ttaaaaaatg
     3781 gctgtagtaa aggaacccat tctttcttca aatcaaactc accattatta atattatcct
     3841 ttaaaggcat tcccaagctt tcaatgacag aaccatcgtc accaaagtct ttagaatact
     3901 cttccccttt aaactcttca gttttcttat caaaccatgt caaacgtaat ttaagcccca
     3961 tatttacctc tcaaagatat tttttaatgt tccgttttgg atctggccct ttaacctgct
     4021 tacccgtttt gggatcaaat gcgcctaggt gttcgccatc actggcacga tacccttcaa
     4081 gctcaccgtg ctgggaatcc cattcataaa ttttacgttt tttatcgcca taccatcggg
     4141 cccgcttacc accaccacct tgtttaggtg ttttaggatc acctcgcttt aactctccaa
     4201 gtccctttat gtcttctgtt ttaggtactg gatgataatc atggccgtag tctttagtcc
     4261 ccttgcgagg cttgttcttt tcctcattta acttattttc tgcatccttc gcttttttct
     4321 ccttttgttt ccggctctcc atcgccccgg taagtgcagc cttatctctg gataaggatt
     4381 cattcattgc gtttaaagat gcctgagccg cttttaattt ctcattctga ttctgaatat
     4441 ccttatcaag atagcgaacc tgatcctgcc acccggagcc atattcatga ggcgggttat
     4501 aagtcacaaa atcatcttta tttgcctctg cctcttttac ctttttatcg gcttcctgaa
     4561 cagcagccct tgcaccgggg agcgccttct ctgtcgatgc aatgcggctg tttaaggtcg
     4621 caatattttt atcttcggca tccagctcag ctttcgcttt atcatagtct cttttcagcc
     4681 cctcttccgg atgagcggcg tcccatgcct gctggcgacg cttttcttcc tcctgacgct
     4741 gttttacctg tgccggggta agaacatctg tcaccgacac ataaaccggc ttctgtccgg
     4801 tctctttcgg gaaacgaata acggcctcac gggagttacc accggctgta aaaccagccg
     4861 gacgtgaatc acctttttca gcaacaatgc cttttggcgg ggctttcgct gtcggaacac
     4921 ctttcggtac gctcacctgc agagacggga gacccggaat cgacacactg aatacccccg
     4981 gacgttttgt cggtttcgca tccaccacag ggacactcat tggccgacct gcgacaaccg
     5041 caatatgctg ccgctcatcc ttcaccacat ccacaacccg ttgtctgaca cgaaccgtcg
     5101 cctggtccag tggtaaagta cttgccggag tctccgtcac cgcatcggcc ggtaatgacg
     5161 tcataatttt tgacatcatt ttcggatcat cttttgcaat ctcagaaggc agcacaccgt
     5221 agatcgcaat cccccacaga ccaaacttaa acggcccctt cagggcagcc agcacatcag
     5281 caacggcggc cgacaacgca tcgccggaaa ctgataaagc cagtccttca gcccccggag
     5341 tagccagggc aggcagaccg aaggccatcg cagtggccga agactgtccg ccaccagagc
     5401 taccgctatg attgccgctg ttaccaccat taccaccggg tttactcccg ccgttaccaa
     5461 actcccccgg attatagtta tgaatatctc cattcggcgt atgcgtcgta ccccagcccg
     5521 gcccgctgtt tggatccgta ccaccacctg atgaagggcc acctttacca gacgtaccgt
     5581 tcacattccc actggcctga ccaccattat gtcccagacc tgaattaccc ggacctcgac
     5641 cgtctccacc actcataccg ttttcctctt tgacatttaa aacaataaat taaagccaac
     5701 atgtcgcggt agggataccc gttaccggat accccccgca cagatcccgg cgtgcgcgat
     5761 ttacgcaccg ggctcctgcc tcgggtgtct ggcggtgaac cgctccacag gccatggatg
     5821 aagaacccga acccttggta gccatgcggc tgccagtttg tttgctttcg tccaggtcgt
     5881 atcatccttc tggctcctgc gcctgagcgc ccggcgccag aggtttgtta cgtgtgtcct
     5941 gaacttctgc atggtgggga agttgcccgg taccgagtga tagttcaggt atccctgaac
     6001 cactctcctg agccattttc cctgttcggg gattgagtaa tgccagcgcc ttcgcagacc
     6061 gtctttgatg gctttcagag ttgccgtcat ccgatcccgg cgggtctttc gtatcagcat
     6121 gaacctgccg ttgcgatctt tcccgctgat gtgcgtgaac ccgaggaagt tgaacgtttc
     6181 tggtttgcct tttcccctga tggcacggtt ttcggcagcg aagcggccga actccatcag
     6241 acgggttttc tccgggtgaa ccgtgagtcc gaactccctc agtctgcgct gcatggctat
     6301 acggaagcgc cgggcatcgt atcgtttgtc gaacccgatg acgatgtcat cggcgtatct
     6361 gaccattacc acattgcctg tggcatagcg acgtcgccac tgatgcgccc acagatcgaa
     6421 gacgtagtgg aggtatatgt ttgccagcag cggtgagatg accgcaccct gtggggtgcc
     6481 ttcctccgtt gctcgccatt gaccctcctc cgacgtcccg gctgtgagcc acttacgtat
     6541 gagcctgatt accctccggt cgccgatccg atgctctgtg aacctgatca gccattcgtg
     6601 gctcaccctg tcgaagaact gactgatgtc ggcatccagt acccagttta cgttagtgcg
     6661 taccagccct gtggccagtg cgtccagtgc atcgtgctgg cttcgcccgg gtctgaaccc
     6721 gtatgagaac cccataaagt cgttttcata gactgcgttc aggattttca ccagcgcata
     6781 ctggacgatc ttgtcctcca gcgaggcgat gccgagcggg cgttgttttc catccgcttt
     6841 tgggatgtag tgacgcctgc cgggctgcgc cctgtagctg ccctgatgta gcctccggtg
     6901 cagatctgtt atgttgttct tcatgtttcc ggcgtagtcc atccacctga tgccatccac
     6961 tccggcggcc gctttcctgc tcagggagag gaatgcggct tccagtgctt cgactgtcag
     7021 caggtggaac aatgctgtaa accgttcttt cttccgctgc ttcgcagctt cccgcacgcg
     7081 tgacagcctc tgtgacatgc tttcccggct ctgtgtccgg cgcatgtgtg gctgttccgc
     7141 gttccccttg gccccgctcc ttcgctccac tgactccgct cctttcgggt tgttcgcctg
     7201 cttcgccgct actatgagcg agtccgactt ctcctctccg tacatcaccg gctatgactc
     7261 ctcgtcttcc cggtgcgggc catctccgac actggcagat ggtcagaggg gagatctccc
     7321 ggttcccgcg tagagatcgt attgacatgc cagggtctca gaccccgccg ggtccatgtg
     7381 gcactcgcag tatcgcaccc tatgatgttg ccttccgtta acagtacaac gtcggcaccc
     7441 ggtaatttaa tatacatttc gtggctcaat ggctggcctg tcaacacccc tgtcaacgct
     7501 tcgccccata cctcgcggta tgcaacgcat gactcgggga ccttgtggat tgctggtcct
     7561 tcaatggtcg gggactttca ccccttgatc tctaaccggt ctcccggcgc acactgtaca
     7621 tatacacagt ataattatta ccttactcct cccgttttca ggtgtcaata tatgagatta
     7681 gttctcatct tcaggcacaa aaaagcgccc ggagccacct gtaagcagat cgcgtctttt
     7741 cccgaaaatt tctcatggca acaaatttta ttatttaaga cattcatcac acttacattt
     7801 cagatggtat agacacatct gaaagctaac tggcatgagg agaggacctg cggttaaact
     7861 gttcgggaaa cctgagacgc ccggaggcca ctatgacgca cctgaaactg actcccgctg
     7921 aagaaaagcg cctggcggac gcctttgtcg ctcaggcact ggccatgggc ctgaaaggga
     7981 acaaaacgct gccaaaggcg gtaaaacaac aggtgcggga aagggtgctt cgtgccattc
     8041 aggacgaacg tgaagccagg cgggcgcagg aaatgcgtcg ggagaaatac gaggctacgg
     8101 aaaccacctt cacctggcag ccacagcgcc gccggtaagg ggtatttacc ataacgtcgc
     8161 attgcccgca ccgctcagac ggtctgaggg cgtttccggc ggctacggga taaaacaggc
     8221 aacaaaccac ccggaaaacc gccggaaacg cgcccggtaa attgtaaccc gctgcatgcc
     8281 tatgcacgca actgcatggc gggccgtttg gtgtaaaaac gatacgataa gccgtgttta
     8341 tgtaaatgaa cgtggccgta aagagtgaca aatatacatg taacattttt tactgacagg
     8401 tcatcaaccc gatgcgttta atacccttta tcctgctggc attgtgcagc cccttactcc
     8461 tgacggcctg taagcattcc accacccagc cgccgaaaat ttatactctg gataagtcat
     8521 ctaaaatcag tagctacgct gcggccatca agaccgctgt tgaaatgcag atgtttgaac
     8581 cagaggccta tgcgggaaaa atgtgcaccg tgcggatatc catacaacgt gatggcactc
     8641 tgaacagcgc cattgctgaa ggaggagatc cgaaactctg taaagcagca atatcagccg
     8701 ttacccacgc caaaattccg ccggcaccgg atgaaaatac ctggcaaata ttcaaaaacg
     8761 cccctctgga tttcagacca tgaaagcttt tactctgaca gtgctggcgg ccatgttcct
     8821 cactccggta catgcgatac cactaaaacc cgtaaccaca ctgcagccaa cctacccggt
     8881 acaggccgca gcaaaacgca tcgaaggtca ggtgaccgcc acgtttgata ttctggacga
     8941 tggacgagtg gacaacgtgt cgcttaccgg ctgtcacctt ttttttcggg aaacgctgac
     9001 ggcaatgagg cactggctat atcccgcagg gcacccggca atgcgacagg ttataaattt
     9061 tgaattcagt ctgaaaaatg acggcttaca ggatccgcga ccagtcacca cccaaaagct
     9121 caatgatgat ggttgccgcc agtaacaggt cctttaaccc gcaggctgca atagtcctgg
     9181 atgatgagtg agcctgtagc agccctgatt agtggaagcg aagctgaaaa atgggcagca
     9241 aaagccgtat ttctggcaaa tagcgccgcc acggattaaa gtagtcatgt gtaatacata
     9301 tgactacaaa ggggctttta tgtcatcagc aacatccatc aggctcgatg aagaacttaa
     9361 ggaccggctg aaaacgctgg ccgatgaccg gcaccgctca gcgcacgcgc taatgctgga
     9421 ggcaatcacc gaatacatcg accgggaaga gaagcgcagc cagtacctgc gggacggaca
     9481 ggccgcatgg cagcactatc aggaaacggg tctgcacctt actgcggaag aagcggaagc
     9541 ctggataagt acctggggaa ctgagaacga gcaggatgct ccgccatgcc acaggtaacg
     9601 atttccgcgc tggcacagcg cgatttacaa cgccttcagg actttctcaa aaccaaaaat
     9661 cggctggcag ccagaaaagg aggtgaggtg atcgtccggg ctatccagca actgaaaaca
     9721 ttgccagaca ttggccgccc ggtgccgttt ctgccgctgg aatataagga actggtgatc
     9781 gggtttggcg acagtggcta tgtgatgctc taccgccacg accgggaaat ggaccagatt
     9841 gtgatcgtca ccgtcaggca tcagaaagaa tccgggtatc cgggggcaga cagcctctaa
     9901 taaccgatga cgggggcgca attcattgcg tggccgtcgc cggtttgaca agcgcagcgc
     9961 gtcagaaaac cacatcctga acggaaaaac gggtaaaatt cagcgataca tttaccgtga
    10021 gaaattacac acctttcaca cccgataaaa tcaggggtat acagtggcga aaacattgct
    10081 caacctttca caggcagcac aagccgcggg cattaccaga agaacgctat acaatcatgt
    10141 aaaacaggga aaggttaccg tgtcacgtga cgggaaaaac aaccctgtcg tggatgtgtc
    10201 agaactgatc cgtgtatacg gaaatgtgaa tatccctgag aaacaaattc ccggcatttc
    10261 acaccgtgaa aatacacaaa aaaatttccc acaggaacag cttctggcaa tgcaaaagga
    10321 actggccgat ctccggcagg ctgtcaccct gatgctggaa gataaaacat cccgggaaga
    10381 agaacgccgc cagcacgatg atgagcgccg gaaactccag gccgaagtgg acagattgac
    10441 aacagaactg acacagaaga aaaaacgctt ctggtccggc tggtttagct gagaacttac
    10501 cgaagggaat ggagaacatg acgaagcagg aaaaaactgc cctcaatatg gctaaattca
    10561 ttaaggatca gtcgctgctg ttgctggaaa agctgaacga gctggacatg gataccccgg
    10621 ccgacatgtg cgaacggctt cacgaggatg cggaaacact ctggcaggtg atgcgggata
    10681 agctcgggga agaataacag caccggccat gccggtgctg gcgttatttt ttgccgaacg
    10741 gccaccaccg gcgggcgggt ttgtcctccg gcagttcagg caccggcacg ttaaaatagc
    10801 ggctgttata ttcccgcatc agctccgctt tcagcttctc gttcaccgcg ctttccccgt
    10861 gccgctcgct gaccacctca atcatcgcgt taatctgctc cggtgagcac ttcccggcat
    10921 caaccacctg cacaccgttc tcgatggcga accgctccac ctttcgatag aggtaatcac
    10981 acagcttcat tcctgtgggt ttgtcccgtt cccattgcgg attttcaccg gtgaaaaagt
    11041 acgtggcttt ttcgtcgctg ttgagccgct cgtcatacat cccgatggat ttcatcatcg
    11101 ccagatgctg ggcccggtcg acaaaacaga aaacatacgc cccggtctgc caggcgagca
    11161 ccaccatgtc ttccagatgc tcgctggttg gcataatgca gcgctcgccg tctttgtctt
    11221 ccacgtcctc aataaatacc atgtacagcg ccataatccc tcactccgtt gtcataaatt
    11281 taaagcacgc gaacagcctc tcccggtcca gctccaccgt tttgcgggcc tcgccggtct
    11341 gtggattcac gatttcatcc accgccagaa tcgcccgctg tacccggaca agcgtcggcc
    11401 ggtcagggca gatgtaccag atttcatcga tgccgttcgc ccggcggttg aacagatacc
    11461 cggacaccac ttcctgatac cgcctgcggc tcttgacggt gcgctctacc tcaaatgcca
    11521 ccgttctgcc gtccagctcc gccagcgcgt ccggcacttt catccctttc agggccatac
    11581 ggtgcaggta ccgccagccg gtggcccccc ggccctccat ggagaggcgc agggactgca
    11641 cggccagccg gtgcggcagc tgcgccgccg acacccttga cggctgaaac ggaataacgt
    11701 ccgtcagcgg ctcgtcctcg tcgaacgaga acgccacccc gtgcggcgtc agcccccaca
    11761 cgctgacctg acggccgtca accgggtatt tcgcccggac cagcagccct ttcgccacca
    11821 gactgtccag gaagcggtaa accggctgta ccgccgcaat ccccagccgt cgcgcaacca
    11881 gctccgccgt ggaacacagc tcttcccgca gccagcgcag ggtcagccgt gttttctccg
    11941 cgttccgcgc ctgccgcttc gcgtggctga
//
