LOCUS       NZ_CP087656             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01653 plasmid
            pDD01653-5, complete sequence.
ACCESSION   NZ_CP087656
VERSION     NZ_CP087656.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822721
            Assembly: GCF_023066765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066765.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:22:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,846
            Genes (coding)                    :: 5,661
            CDSs (with protein)               :: 5,661
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 69 of 185
            Pseudo Genes (incomplete)         :: 129 of 185
            Pseudo Genes (internal stop)      :: 23 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087656.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01653"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01653-5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            107..616
                     /locus_tag="LL272_RS29650"
                     /old_locus_tag="LL272_29650"
     CDS             107..616
                     /locus_tag="LL272_RS29650"
                     /old_locus_tag="LL272_29650"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            623..835
                     /locus_tag="LL272_RS29655"
                     /old_locus_tag="LL272_29655"
     CDS             623..835
                     /locus_tag="LL272_RS29655"
                     /old_locus_tag="LL272_29655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1502..1726
                     /locus_tag="LL272_RS29660"
                     /old_locus_tag="LL272_29660"
     CDS             1502..1726
                     /locus_tag="LL272_RS29660"
                     /old_locus_tag="LL272_29660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1710..1976
                     /locus_tag="LL272_RS29665"
                     /old_locus_tag="LL272_29665"
     CDS             1710..1976
                     /locus_tag="LL272_RS29665"
                     /old_locus_tag="LL272_29665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2046..2396)
                     /locus_tag="LL272_RS29670"
                     /old_locus_tag="LL272_29670"
     CDS             complement(2046..2396)
                     /locus_tag="LL272_RS29670"
                     /old_locus_tag="LL272_29670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2462..3007)
                     /locus_tag="LL272_RS29675"
                     /old_locus_tag="LL272_29675"
     CDS             complement(2462..3007)
                     /locus_tag="LL272_RS29675"
                     /old_locus_tag="LL272_29675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(3033..3359)
                     /locus_tag="LL272_RS29680"
                     /old_locus_tag="LL272_29680"
     CDS             complement(3033..3359)
                     /locus_tag="LL272_RS29680"
                     /old_locus_tag="LL272_29680"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3717..3875)
                     /locus_tag="LL272_RS29890"
     CDS             complement(3717..3875)
                     /locus_tag="LL272_RS29890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4515..4691
                     /locus_tag="LL272_RS29685"
                     /old_locus_tag="LL272_29685"
     CDS             4515..4691
                     /locus_tag="LL272_RS29685"
                     /old_locus_tag="LL272_29685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4681..5025
                     /locus_tag="LL272_RS29690"
                     /old_locus_tag="LL272_29690"
     CDS             4681..5025
                     /locus_tag="LL272_RS29690"
                     /old_locus_tag="LL272_29690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5207..5452)
                     /locus_tag="LL272_RS29695"
                     /old_locus_tag="LL272_29695"
     CDS             complement(5207..5452)
                     /locus_tag="LL272_RS29695"
                     /old_locus_tag="LL272_29695"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4350..4429
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3767..4164)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2..423
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="86.0"
                     /evalue="9.65e-84"
     Typing          5016..5596
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.2900000000000002e-122"
ORIGIN
        1 gaggcgctgg aaagcgcagg ttttgaggtc gtgcgcacca caaagagcag catcagcatt
       61 gccgacccgg acggagggcg aaacatccga cttaagggag ccatctatga acagtctttt
      121 aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagagta ccggcgaaat
      181 gctgaaagcc gcattcagcg agcacgagaa gtctgtcaga gcggaactga gcgaaagcgg
      241 gaagagaatc agcgccgcca tccgcgccca cgagcagggt atgagcgcgg ccatgcagtc
      301 gaaccgcctg agcgtgatgc gtatggtcgg gcggacatgg ctgaccatca cgatggtgtc
      361 aggactgctg ttcgccagcc tgagcggggt gttgtggtat caggggagct tgatagcgtc
      421 aaatctggcc gaaatcgacc ggcagaacgc agcgctgtcg aagctgaacg cgaagacctg
      481 gggcgtgacg tacctggaag acagcaacgg acgttttctg gtgctgccga aggggacggc
      541 cgtagacagg acgcagagct ggacggtcgg gaacgggcgg tcgaagcaga acgcgctgag
      601 actggtgaag gagtaagtgc cgatgaccga gctggaaaaa cagttgctga gcgcattaga
      661 gcagctacag caggactact cgaaaaggct ggacgagtgg gagagcgcct tcggggaatg
      721 gcggaaaatg tcagggctta tgcaacggga gaacgcggcg ctgagcgagc gcgtgacgcg
      781 cttgagtcag caggtggaac gcttgagcgg gcaactgcag cgtttgagcc ggtagtgcag
      841 cgtcatgaga tggccgtggc cgcagaacgg gcgcacgagc aacggcagca cgaaaaggaa
      901 ctgacggaag cgcgttcccg gcagcgaagc tatgacggtc cgtcgctggg ctgaaatgca
      961 ggaaaacagg gttctcatcg ggcatttacg ctgaaaccgg atgtcattca cctggctgca
     1021 tggctatgca gccaggtaaa atttatctgt cgcgaacggg gcggtttatc gggtggtttg
     1081 ttgccggttt tacctgactg ccaccccgtt cgcggcgaac ccgtccgggg cggtgcgggc
     1141 aacgggtgtt atgtttaatc agcgtgaacg gcagatacaa aaaagccccg gagggctttt
     1201 aactactgac ttattttttt gaagatttgc tgggtttcaa tttgtcagta tttcccggcg
     1261 tttttttatt gtcacgctgg cgtttatcag gttcccctgt tgaatcggca attcgtttcg
     1321 gacccggctt aagacccata atcgttatcc tttcaatagt taagaggaaa gccctcaagc
     1381 tagatatatt gaccgcgtta cggattatca agaggggcta acaagattat tttttgttct
     1441 tttaggttga ttgtggtact gttgatatac atgtatagca aatggtgttc gggaggttta
     1501 tatgcttgct atccggttat ctgatgagat tgagtcccgt ctggactcgc tggcgaagca
     1561 aaccggcaga acaaagacgt tttatgcgcg ggaagcaata ctggcgcatc tggaagacct
     1621 ggaggattat tatctttcag cagaaactgc tgcacgcgtt cgccgtggtg atgaagcagt
     1681 gcattcgtct gaagacgtga ggaagtcact tggtctggac gattaactat tccgatcggg
     1741 cgctcaaatc gttacgcaag atggacaaac agaacgcacg acggattgtg gattttatga
     1801 gtttacgcat tgcagttgct gccgatcctc gccagtcagg gaagccgctc aaaggtgagc
     1861 tgggcgagtt ctggcgctat cgggtgggag attatcgcgt tctgtgtgag atccgagatg
     1921 acgagcttgt tatccttgcc gccacgattg gacatcgccg cgaagtttac gactgaagcc
     1981 cgctatgcgg gcttttttga tgccgacaac aggtattatg gtaaatactc gactaactac
     2041 gtgttctact gtttgtcggt tttagttaac cagcaggttg tcttaacgat aatgtagccg
     2101 ttatttttaa ctaataaggt catattgtta acgtcaggca taataaaaga cattatggcc
     2161 tcagagttat catcagaagc agctctctgc attactactt cgattccctt ttctttgaat
     2221 ctataagtat ccagttgcct attaaactta gaaagaccta caaccatact tagtttgtta
     2281 tctttaacag taagagatgc ttcctgagta ccattacatc ggtagtaagc ggtgtaaccg
     2341 tcagcaaaag atcgaccggg gaatataaat gctaggaacg caattaacca atacatagtt
     2401 gagaatcttg gtattacaca tttcactatc ctctctttca tttttcgttc accatacttt
     2461 gttatctgat ttttaatttt tctattttat aaaccaagtc tctaccaaaa aggcgaaaat
     2521 tatcctcaaa taataataat tgtttaagaa catccatgaa agcagctgct tcaccctcag
     2581 ttatgggagc tactctatat aacatataac ctgagttaag ggtttctttt atactttctt
     2641 caatgctcat tataaatatt agtggattat catttttctt cccccatggg tttctaatta
     2701 atccttcgat gtactcaggt cctttgcgtc ctattgcctg cgcaatattt tttatttcat
     2761 ctgcgtgagg gctttgttgt acagctttgt ttatgcctgc ttgagtgatt ttcagagact
     2821 ctacaccgtg tatgttgtat gagataaaat gtaataaatt aagatgcaat gcaattaatc
     2881 catttttgtt tacatctgag tatttggata aaaaacgtaa tgaagtgtta actctccctt
     2941 gaagcctaac aattgggagt gctgccttgt atatttcctt ctccgttaaa taccgtttaa
     3001 aaaacataat tctccgcctc cgtatagcgt gcctatttta tttcgtcgta aaacttagct
     3061 ctgaaatatt cctttagaac catcatgtca ttaataattg aatctatctc atccattttt
     3121 tcttggtatt cttttaatgg tgaagggagc ggtaactcaa tatttgaaaa gtgattggcg
     3181 agatagggat caaggtttct catccattca ggcttttcgc caatgcgttc ttcaactgaa
     3241 agtgaacctg ataatctctg gttaataaga tcacttagac tcattttggt tttattcgaa
     3301 agccgttgta aggattgctt taatcctata tctagcatga tatttgttct ttctctcatt
     3361 accccccctt gctattgcgt tttttgttgc gcacaaagct accattgcgc attaagaaac
     3421 gcaataaaaa aggagggagg gagtggtttg tacatgatgt gcagtgcgct gagggcatca
     3481 gtggcgggtg tttttccgtg ggatggatgg agctccgggg ataacagaac tgacgatcat
     3541 ttaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctcttgcact gtaaacgaaa
     3601 aaaccgcctg gggaggcggt ttgatcgaag gttaagtcag ttggggaact gcttaaccgg
     3661 gtaactgggt atacgagacc gcagatacca aaactgttct ttcagtgtag ccgcagttag
     3721 gcacccactt caagacctct caatatctct ctcgtatata tccagttacc aatggctgct
     3781 gccagtggcg tttttgcgtg tccgtccggg ttggactcaa gatgatagtt accggaaagg
     3841 gcgcagcagt cgggctgaac ggggggttcg tgcatacagc ccagcttgga gcgaacgacc
     3901 taaaccgagc cgagatacca gcagcgtgag ctatgagaaa gcgccacgct tcccgaaagg
     3961 gaaaaaggcg gaacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcacgaggg
     4021 agccgccgga gagaaatcac cggcatcttt aagtcctgtc gggtttcgtc ctcctctgac
     4081 ttgagcgtcc atttctgtga tgctcgtcag gggaggcgga gcctatggaa aaacggcaac
     4141 gccgcatcct tttctccggc cctttctgat ttgagcgcct ttgctttatc ccgttatccg
     4201 tgagtcggtc acgcccgccg cagccgaacg gcaggagcgc agctaccgag tgagcgagga
     4261 ggcgtcattt catctgtccg tgcatggtat tgcggacgct cctgagccac atatcacacc
     4321 tgccacatga ggcactttca tgaatcgtgc tctcatgcca acatagtaag ccagtataca
     4381 cagcgtaaac gctgtgactg gttcagggct gcgccccgaa acccgctaac accactgacg
     4441 cgctggcgct tgtccaacgc cggagcgacc cggaaaactt ttccgtgccg tccggcgttt
     4501 gcgaaggagg gataatgcag aagccgaaga agaagccgct cgtcgtaaaa aaaagatcct
     4561 ggcatccggc agtgatggca aatctgatgc gtcaggcaca ggaggaagat taccgccggg
     4621 agcagatggc agagcttgag cgttcagccc ggtcacattt tgcggatatc ggaggagagg
     4681 atggcggtta agcgtacccg ttttttaggt atccgggtga ccgatgggga gtaccagcag
     4741 ctgctggagc gctgcaatgg aaggcaactg gcggtgtgga tgcgggaaac ctgtctcgat
     4801 acgcgcccgg cgcggtcgtt gcggctgccg tccatcgatc cggttttgct gcgccagctt
     4861 gccgggatgg ggaacaacct caaccagatc gcccggaaaa ttaacggcgg ccagtggtca
     4921 ggcgctgacc gggttcaggt ggtggccgcg ctgatggcca ttgatgccga actcgagcgg
     4981 ctgcggcatg ccgtgctgga aaagggggca gacgatgatc gttaagtttc atccgcgagg
     5041 gcgaggcggc ggtgccgggc cggtggatta tctgctgggg aaagaccgcc agcgcgacgg
     5101 ggccatcgtc ctgcagggaa agccggagga ggtccgggag cttatcgacg cctcgcccta
     5161 cgccaaaaag tacacctccg gcgtcctgtc ttttgctgaa caggatttac cgcccggcca
     5221 gcgtgaaaag ctgatggcga gcttcgagcg ggttctgatg cccggactcg ataaagacca
     5281 gtacagcgtg ctgtgggttg aacaccggga caaggggcgg ctggaactga attttctggt
     5341 accgaacacg gaactgctga ccggcaggcg tctccagccg tactacgacc gggcagaccg
     5401 tccgcgcatc gatgcgtggc agaccgtggt gaacgggcga ctggggctgc acgacccgaa
     5461 cgcaccggag aaccggcgcg cgctggtgac gccatcttcg ttacccaaaa cgaagcagga
     5521 agccgcagag gctattacgc ggggcttact ggccctcgcc tcgtcagggg agcttaaaac
     5581 gcgtcaggac gtcact
//
