LOCUS       NZ_CP087655            11970 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01653 plasmid
            pDD01653-4, complete sequence.
ACCESSION   NZ_CP087655
VERSION     NZ_CP087655.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822721
            Assembly: GCF_023066765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066765.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:22:45
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,846
            Genes (coding)                    :: 5,661
            CDSs (with protein)               :: 5,661
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 185
            CDSs (without protein)            :: 185
            Pseudo Genes (ambiguous residues) :: 0 of 185
            Pseudo Genes (frameshifted)       :: 69 of 185
            Pseudo Genes (incomplete)         :: 129 of 185
            Pseudo Genes (internal stop)      :: 23 of 185
            Pseudo Genes (multiple problems)  :: 35 of 185
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087655.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01653"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01653-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            join(11335..11970,1..1329)
                     /locus_tag="LL272_RS29580"
                     /old_locus_tag="LL272_29580"
     CDS             join(11335..11970,1..1329)
                     /locus_tag="LL272_RS29580"
                     /old_locus_tag="LL272_29580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            1329..2060
                     /gene="mobC"
                     /locus_tag="LL272_RS29585"
                     /old_locus_tag="LL272_29585"
     CDS             1329..2060
                     /gene="mobC"
                     /locus_tag="LL272_RS29585"
                     /old_locus_tag="LL272_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            2067..2597
                     /locus_tag="LL272_RS29590"
                     /old_locus_tag="LL272_29590"
     CDS             2067..2597
                     /locus_tag="LL272_RS29590"
                     /old_locus_tag="LL272_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2624..2803)
                     /locus_tag="LL272_RS29595"
                     /old_locus_tag="LL272_29595"
     CDS             complement(2624..2803)
                     /locus_tag="LL272_RS29595"
                     /old_locus_tag="LL272_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2829..3257)
                     /locus_tag="LL272_RS29600"
                     /old_locus_tag="LL272_29600"
     CDS             complement(2829..3257)
                     /locus_tag="LL272_RS29600"
                     /old_locus_tag="LL272_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(3421..3735)
                     /locus_tag="LL272_RS29605"
                     /old_locus_tag="LL272_29605"
     CDS             complement(3421..3735)
                     /locus_tag="LL272_RS29605"
                     /old_locus_tag="LL272_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(3723..4001)
                     /locus_tag="LL272_RS29610"
                     /old_locus_tag="LL272_29610"
     CDS             complement(3723..4001)
                     /locus_tag="LL272_RS29610"
                     /old_locus_tag="LL272_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(4176..4541)
                     /locus_tag="LL272_RS29615"
                     /old_locus_tag="LL272_29615"
     CDS             complement(4176..4541)
                     /locus_tag="LL272_RS29615"
                     /old_locus_tag="LL272_29615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(4538..4909)
                     /gene="tolA"
                     /locus_tag="LL272_RS29620"
                     /old_locus_tag="LL272_29620"
     CDS             complement(4538..4909)
                     /gene="tolA"
                     /locus_tag="LL272_RS29620"
                     /old_locus_tag="LL272_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(5183..5428)
                     /locus_tag="LL272_RS29625"
                     /old_locus_tag="LL272_29625"
     CDS             complement(5183..5428)
                     /locus_tag="LL272_RS29625"
                     /old_locus_tag="LL272_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6073..7560
                     /gene="ltrA"
                     /locus_tag="LL272_RS29630"
                     /old_locus_tag="LL272_29630"
     CDS             6073..7560
                     /gene="ltrA"
                     /locus_tag="LL272_RS29630"
                     /old_locus_tag="LL272_29630"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            7665..9350
                     /locus_tag="LL272_RS29635"
                     /old_locus_tag="LL272_29635"
     CDS             7665..9350
                     /locus_tag="LL272_RS29635"
                     /old_locus_tag="LL272_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9360..9617
                     /locus_tag="LL272_RS29640"
                     /old_locus_tag="LL272_29640"
     CDS             9360..9617
                     /locus_tag="LL272_RS29640"
                     /old_locus_tag="LL272_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            9702..9851
                     /locus_tag="LL272_RS29645"
                     /old_locus_tag="LL272_29645"
     CDS             9702..9851
                     /locus_tag="LL272_RS29645"
                     /old_locus_tag="LL272_29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     BGC             2665..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(9955..10533)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          1330..2057
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 catttcgggg agccggtacc ggttaacgcg ctggccgatt tttccacgga gcaggcccgg
       61 aaacagcagg cggtgtttct gggtaaggat gagcagggcc agccggtgct ggtccctcgt
      121 gatacctggc gcaagaccaa tattcagatc ctcggcctgc ccgggagcgg caagagcgcg
      181 atggccacca acgcgctcat tcgctgcgta cgcgatttcg gggacgctgt ggtgtacttc
      241 gaccccaaag gggacgcgtg ggcgccgcac gtcttccggg cgcactgccc ggacttcacc
      301 ctgctggatc tgcggccggg gaaaccggcg cagctgaatc tgttccgcga tctcgaccag
      361 tacgccctga agaatctgct ggttgccggg tttaacctga gtgagaccgg cgacgtggcg
      421 gaccactacc gcatcagcga gcagaaagcg gcgaagctga ttgcggagca gttcccgcag
      481 ggggcaaaca ttcagcaggt gctggcggcg gcgtacgcgt tgccggaggc cctgaaaaag
      541 gacgtgaagg ggctgatcac caagctggag aacgtggccg acctgagcgt cctgcagacg
      601 gacagcggta tcgatgtggc ggggataatc aacggcggcg gctgcctgta cgtcatcggc
      661 tcgatggacg atgaggcggt gatccgggtg cagaagatgc tgtttgcccg ctgcgcgcaa
      721 atcatcattg cgcgggatga gttccggagg tggccgcacg cgagcatcat gctcgatgag
      781 attaaatatc tgctgtcgaa gtacgtgctg aacgcgctgg ggacgctgcg cagccgggac
      841 tgcaacctgc tgctcgcgca ccagtcgctg ggcgacttcg gtcagtgcgg gcaggatctg
      901 cccgctgatt ttgtgaaaac cacggtactg gataacacgc cgatccgctg gttctatcgc
      961 gcggccagcc aggagtccgc gcagtgggcg gcgggccaga ccggggagat ccgggtggac
     1021 gtggagcgcc gccgggccag ccgggaggcg gggaacgtgg agcacatcag cggcgacagc
     1081 ttcatccaga aggaggcgcg gccgctgttt gacgtgaaca ccctgcaaca cctgcctgac
     1141 gggttcgcgg tgatgaccgg gctgggcgtg gcgcggctgg cgttcagcag cccgctgcgg
     1201 gttgagcgcc gggaaatttc gctgaaaacc ttcccggtgc tggcgaaaac ggacccgctg
     1261 gcggagtacc tgcctccggc cgacggcggc ggaccaaaag cggatgatga ttttgcgggg
     1321 ctttactgat gacactggag cagtacaacg tcagccacgc gaagcggcag gcgcggaacg
     1381 cggagaaaac acggctgacc ctgcgctggc tgcgggaaga gctgtgttcc acggcggagc
     1441 tggttgcgcg acggctgggg attgcggcgg tacagccggt ttaccgcttc ctggacagtc
     1501 tggtggcgaa agggctgctg gtccgggcga aatacccggt tgacggccgt caggtcagcg
     1561 tgtgggggct gacgccgcac ggggtggcgt tctcgttcga cgaggacgag ccgctgacgg
     1621 acgttattcc gtttcagccg tcaagggtgt cggcggcgca gctgccgcac cggctggccg
     1681 tgcagtccct gcgcctctcc atggagggcc ggggggccac cggctggcgg tacctgcacc
     1741 gtatggccct gaaagggatg aaagtgccgg acgcgctggc ggagctggac ggcagaacgg
     1801 tggcatttga ggtagagcgc accgtcaaga gccgcaggcg gtatcaggaa gtggtgtccg
     1861 ggtatctgtt caaccgccgg gcgaacggca tcgatgaaat ctggtacatc tgccctgacc
     1921 ggccgacgct tgtccgggta cagcgggcga ttctggcggt ggatgaaatc gtgaatccac
     1981 agaccggcga ggcccgcaaa acggtggagc tggaccggga gaggctgttc gcgtgcttta
     2041 aatttatgac aacggagtga gggattatgg cgctgtacat ggtatttatt gaggacgtgg
     2101 aagacaaaga cggcgagcgc tgcattatgc caaccagcga gcatctggaa gacatggtgg
     2161 tgctcgcctg gcagaccggg gcgtatgttt tctgttttgt cgaccgggcc cagcatctgg
     2221 cgatgatgaa atccatcggg atgtatgacg agcggctcaa cagcgacgaa aaagccacgt
     2281 actttttcac cggtgaaaat ccgcaatggg aacgggacaa acccacagga atgaagctgt
     2341 gtgattacct ctatcgaaag gtggagcggt tcgccatcga gaacggtgtg caggtggttg
     2401 atgccgggaa gtgctcaccg gagcagatta acgcgatgat tgaggtggtc agcgagcggc
     2461 acggggaaag cgcggtgaac gagaagctga aagcggagct gatgcgggaa tataacagcc
     2521 gctattttaa cgtgccggtg cctgaactgc cggaggacaa acccgcccgc cggtggtggc
     2581 cgttcggcaa aaaataacgc cagcaccggc atggccggtg ctgttattct tccccgagct
     2641 tatcccgcat cacctgccag agtgtttccg catcctcgtg aagccgttcg cacatgtcgg
     2701 ccggggtatc catgtccagc tcgttcagct tttccagcaa cagcagcgac tgatccttaa
     2761 tgaatttagc catattgagg gcagtttttt cctgcttcgt catgttctcc attcccttcg
     2821 gtaagttctc agctaaacca gccggaccag aagcgttttt tcttctgtgt cagttctgtt
     2881 gtcaatctgt ccacttcggc ctggagtttc cggcgctcat catcgtgctg gcggcgttct
     2941 tcttcccggg atgttttatc ttccagcatc agggtgacag cctgccggag atcggccagt
     3001 tccttttgca ttgccagaag ctgttcctgt gggaaatttt tttgtgtatt ttcacggtgt
     3061 gaaatgccgg gaatttgttt ctcagggata ttcacatttc cgtatacacg gatcagttct
     3121 gacacatcca cgacagggtt gtttttcccg tcacgtgaca cggtaacctt tccctgtttt
     3181 acatgattgt atagcgttct tctggtaatg cccgcggctt gtgctgcctg tgaaaggttg
     3241 agcaatgttt tcgccactgt atacccctga ttttatcggg tgtgaaaggt gtgtaatttc
     3301 tcacggtaaa tgtatcgctg aattttaccc gtttttccgt tcaggatgtg gttttctgac
     3361 gcgctgcgct tgtcaaaccg gcgacggcca cgcaatgaat tgcgcccccg tcatcggtta
     3421 ttagaggctg tctgcccccg gatacccgga ttctttctga tgcctgacgg tgacgatcac
     3481 aatctggtcc atttcccggt cgtggcggta gagcatcaca tagccactgt cgccaaaccc
     3541 gatcaccagt tccttatatt ccagcggcag aaacggcacc gggcggccaa tgtctggcaa
     3601 tgttttcagt tgctggatag cccggacgat cacctcacct ccttttctgg ctgccagccg
     3661 atttttggtt ttgagaaagt cctgaaggcg ttgtaaatcg cgctgtgcca gcgcggaaat
     3721 cgttacctgt ggcatggcgg agcatcctgc tcgttctcag ttccccaggt acttatccag
     3781 gcttccgctt cttccgcagt aaggtgcaga cccgtttcct gatagtgctg ccatgcggcc
     3841 tgtccgtccc gcaggtactg gctgcgcttc tcttcccggt cgatgtattc ggtgattgcc
     3901 tccagcatta gcgcgtgcgc tgagcggtgc cggtcatcgg ccagcgtttt cagccggtcc
     3961 ttaagttctt catcgagcct gatggatgtt gctgatgaca taaaagcccc tttgtagtca
     4021 tatgtattac acatgactac tttaatccgt ggcggcgcta tttgccagaa atacggcttt
     4081 tgctgcccat ttttcagctt cgcttccact aatcagggct gctacaggct cactcatcat
     4141 ccaggactat tgcagcctgc gggttaaagg acctgttact ggcggcaacc atcatcattg
     4201 agcttttggg tggtgactgg tcgcggatcc tgtaagccgt catttttcag actgaattca
     4261 aaatttataa cctgtcgcat tgccgggtgc cctgcgggat atagccagtg cctcattgcc
     4321 gtcagcgttt cccgaaaaaa aaggtgacag ccggtaagcg acacgttgtc cactcgtcca
     4381 tcgtccagaa tatcaaacgt ggcggtcacc tgaccttcga tgcgttttgc tgcggcctgt
     4441 accgggtagg ttggctgcag tgtggttacg ggttttagtg gtatcgcatg taccggagtg
     4501 aggaacatgg ccgccagcac tgtcagagta aaagctttca tggtctgaaa tccagagggg
     4561 cgtttttgaa tatttgccag gtattttcat ccggtgccgg cggaattttg gcgtgggtaa
     4621 cggctgatat tgctgcttta cagagtttcg gatctcctcc ttcagcaatg gcgctgttca
     4681 gagtgccatc acgttgtatg gatatccgca cggtgcacat ttttcccgca taggcctctg
     4741 gttcaaacat ctgcatttca acagcggtct tgatggccgc agcgtagcta ctgattttag
     4801 atgacttatc cagagtataa attttcggcg gctgggtggt ggaatgctta caggccgtca
     4861 ggagtaaggg gctgcacaat gccagcagga taaagggtat taaacgcatc gggttgatga
     4921 cctgtcagta aaaaatgtta catgtatatt tgtcactctt tacggccacg ttcatttaca
     4981 taaacacggc ttatcgtatc gtttttacac caaacggccc gccatgcagt tgcgtgcata
     5041 ggcatgcagc gggttacaat ttaccgggcg cgtttccggc ggttttccgg gtggtttgtt
     5101 gcctgtttta tcccgtagcc gccggaaacg ccctcagacc gtctgagcgg tgcgggcaat
     5161 gcgacgttat ggtaaatacc ccttaccggc ggcgctgtgg ctgccaggtg aaggtggttt
     5221 ccgtagcctc gtatttctcc cgacgcattt cctgcgcccg cctggcttca cgttcgtcct
     5281 gaatggcacg aagcaccctt tcccgcacct gttgttttac cgcctttggc agcgttttgt
     5341 tccctttcag gcccatggcc agtgcctgag cgacaaaggc gtccgccagg cgcttttctt
     5401 cagcgggagt cagtttcagg tgcgtcatag tggcctccgg gcgtctcagg tttcccgaac
     5461 agtttaaccg caggtcctct cctcatgcca gttagctttc agatgtgtct ataccatctg
     5521 aaatgtaagt gtgatgaatg tcttaaataa taaaatttgt tgccatgaga aattttcggg
     5581 aaaagacgcg atctgcttac aggtggctcc gggcgctttt ttgtgcctga agatgagaac
     5641 taatctcata tattgacacc tgaaaacggg aggagtaagg taataattat actgtgtata
     5701 tgtacagtgt gcgccgggag accggttaga gatcaagggg tgaaagtccc cgaccattga
     5761 aggaccagca atccacaagg tccccgagtc atgcgttgca taccgcgagg tatggggcga
     5821 agcgttgaca ggggtgttga caggccagcc attgagccac gaaatgtata ttaaattacc
     5881 gggtgccgac gttgtactgt taacggaagg caacatcata gggtgcgaca ctgcgagtgc
     5941 cacatggacc cggcggggtc tgagaccctg gcatgtcaat acgatctcta cgcgggaacc
     6001 gggagatctc ccctctgacc atctgccagt gtcggagatg gcccgcaccg ggaagacgag
     6061 gagtcatagc cggtgatgta cggagaggag aagtcggact cgctcatagt agcggcgaag
     6121 caggcgaaca acccgaaagg agcggagtca gtggagcgaa ggagcggggc caaggggaac
     6181 gcggaacagc cacacatgcg ccggacacag agccgggaaa gcatgtcaca gaggctgtca
     6241 cgcgtgcggg aagctgcgaa gcagcggaag aaagaacggt ttacagcatt gttccacctg
     6301 ctgacagtcg aagcactgga agccgcattc ctctccctga gcaggaaagc ggccgccgga
     6361 gtggatggca tcaggtggat ggactacgcc ggaaacatga agaacaacat aacagatctg
     6421 caccggaggc tacatcaggg cagctacagg gcgcagcccg gcaggcgtca ctacatccca
     6481 aaagcggatg gaaaacaacg cccgctcggc atcgcctcgc tggaggacaa gatcgtccag
     6541 tatgcgctgg tgaaaatcct gaacgcagtc tatgaaaacg actttatggg gttctcatac
     6601 gggttcagac ccgggcgaag ccagcacgat gcactggacg cactggccac agggctggta
     6661 cgcactaacg taaactgggt actggatgcc gacatcagtc agttcttcga cagggtgagc
     6721 cacgaatggc tgatcaggtt cacagagcat cggatcggcg accggagggt aatcaggctc
     6781 atacgtaagt ggctcacagc cgggacgtcg gaggagggtc aatggcgagc aacggaggaa
     6841 ggcaccccac agggtgcggt catctcaccg ctgctggcaa acatatacct ccactacgtc
     6901 ttcgatctgt gggcgcatca gtggcgacgt cgctatgcca caggcaatgt ggtaatggtc
     6961 agatacgccg atgacatcgt catcgggttc gacaaacgat acgatgcccg gcgcttccgt
     7021 atagccatgc agcgcagact gagggagttc ggactcacgg ttcacccgga gaaaacccgt
     7081 ctgatggagt tcggccgctt cgctgccgaa aaccgtgcca tcaggggaaa aggcaaacca
     7141 gaaacgttca acttcctcgg gttcacgcac atcagcggga aagatcgcaa cggcaggttc
     7201 atgctgatac gaaagacccg ccgggatcgg atgacggcaa ctctgaaagc catcaaagac
     7261 ggtctgcgaa ggcgctggca ttactcaatc cccgaacagg gaaaatggct caggagagtg
     7321 gttcagggat acctgaacta tcactcggta ccgggcaact tccccaccat gcagaagttc
     7381 aggacacacg taacaaacct ctggcgccgg gcgctcaggc gcaggagcca gaaggatgat
     7441 acgacctgga cgaaagcaaa caaactggca gccgcatggc taccaagggt tcgggttctt
     7501 catccatggc ctgtggagcg gttcaccgcc agacacccga ggcaggagcc cggtgcgtaa
     7561 atcgcgcacg ccgggatctg tgcggggggt atccggtaac gggtatccct accgcgacat
     7621 gttggcttta atttattgtt ttaaatgtca aagaggaaaa cggtatgagt ggtggagacg
     7681 gtcgaggtcc gggtaattca ggtctgggac ataatggtgg tcaggccagt gggaatgtga
     7741 acggtacgtc tggtaaaggt ggcccttcat caggtggtgg tacggatcca aacagcgggc
     7801 cgggctgggg tacgacgcat acgccgaatg gagatattca taactataat ccgggggagt
     7861 ttggtaacgg cgggagtaaa cccggtggta atggtggtaa cagcggcaat catagcggta
     7921 gctctggtgg cggacagtct tcggccactg cgatggcctt cggtctgcct gccctggcta
     7981 ctccgggggc tgaaggactg gctttatcag tttccggcga tgcgttgtcg gccgccgttg
     8041 ctgatgtgct ggctgccctg aaggggccgt ttaagtttgg tctgtggggg attgcgatct
     8101 acggtgtgct gccttctgag attgcaaaag atgatccgaa aatgatgtca aaaattatga
     8161 cgtcattacc ggccgatgcg gtgacggaga ctccggcaag tactttacca ctggaccagg
     8221 cgacggttcg tgtcagacaa cgggttgtgg atgtggtgaa ggatgagcgg cagcatattg
     8281 cggttgtcgc aggtcggcca atgagtgtcc ctgtggtgga tgcgaaaccg acaaaacgtc
     8341 cgggggtatt cagtgtgtcg attccgggtc tcccgtctct gcaggtgagc gtaccgaaag
     8401 gtgttccgac agcgaaagcc ccgccaaaag gcattgttgc tgaaaaaggt gattcacgtc
     8461 cggctggttt tacagccggt ggtaactccc gtgaggccgt tattcgtttc ccgaaagaga
     8521 ccggacagaa gccggtttat gtgtcggtga cagatgttct taccccggca caggtaaaac
     8581 agcgtcagga ggaagaaaag cgtcgccagc aggcatggga cgccgctcat ccggaagagg
     8641 ggctgaaaag agactatgat aaagcgaaag ctgagctgga tgccgaagat aaaaatattg
     8701 cgaccttaaa cagccgcatt gcatcgacag agaaggcgct ccccggtgca agggctgctg
     8761 ttcaggaagc cgataaaaag gtaaaagagg cagaggcaaa taaagatgat tttgtgactt
     8821 ataacccgcc tcatgaatat ggctccgggt ggcaggatca ggttcgctat cttgataagg
     8881 atattcagaa tcagaatgag aaattaaaag cggctcaggc atctttaaac gcaatgaatg
     8941 aatccttatc cagagataag gctgcactta ccggggcgat ggagagccgg aaacaaaagg
     9001 agaaaaaagc gaaggatgca gaaaataagt taaatgagga aaagaacaag cctcgcaagg
     9061 ggactaaaga ctacggccat gattatcatc cagtacctaa aacagaagac ataaagggac
     9121 ttggagagtt aaagcgaggt gatcctaaaa cacctaaaca aggtggtggt ggtaagcggg
     9181 cccgatggta tggcgataaa aaacgtaaaa tttatgaatg ggattcccag cacggtgagc
     9241 ttgaagggta tcgtgccagt gatggcgaac acctaggcgc atttgatccc aaaacgggta
     9301 agcaggttaa agggccagat ccaaaacgga acattaaaaa atatctttga gaggtaaata
     9361 tggggcttaa attacgtttg acatggtttg ataagaaaac tgaagagttt aaaggggaag
     9421 agtattctaa agactttggt gacgatggtt ctgtcattga aagcttggga atgcctttaa
     9481 aggataatat taataatggt gagtttgatt tgaagaaaga atgggttcct ttactacagc
     9541 cattttttaa aaatcaaata aaacctgatg tggataattg ttatatagct tttgattatc
     9601 gggacggtaa ctggtaacaa aaaattatca aggatgattt gaaatactga cttccggtgt
     9661 tcagggatga atgccggagt ctttaaatac agggaaagta tatgaaaaag gtaaagacta
     9721 tttttctttt tatattgatt gttgctggct ttcttctggc cgcatgtcag gcaaattata
     9781 tccgggatgt tcagggcgga acggtggcac catcgtcctc ctctgaactg acggggatcg
     9841 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctt
     9901 ttgccctgta aacgaaaaaa ccacctgggg aggcggtttg atcgaaggtt aagtcagttg
     9961 gggaactgct taaccgtggt aactggcttt cgcagagcac agcaaccaaa tctgtccttc
    10021 cagtgtagcc ggactttggc gcacacttca agagcaaccg cgtgtttagc taaacaaatc
    10081 ctctgcgaac tcccagttac caatggctgc tgccagtggc gttttgcgtg ctttcccggg
    10141 ttggactcaa gtgaacagtt accggataag gcgcagcagt cgggctgaac ggggagttct
    10201 tgcttacagc ccagcttgga gcgaacgacc tacaccgagc cgagatacca gtgtgtgagc
    10261 tatgagaaag cgccacactt cccgcaaggg agaacggcgg acaggtatcc ggtaaacggc
    10321 agggtcggaa caggagagcg caagagggag cgacctaccg gaaacggtgg ggatctttaa
    10381 gtcctgtcgg gtttcgcccg tactgtcaga ttcatggttg agcctcacgg ctcccacaga
    10441 tgcaccgaaa aagcgtctgt ttatgtgaac tctggcagga gggcggagcc tatggaaaaa
    10501 cgccaccggc gcggccctgc cgttttgcct cacatgttag tcccctgctt atccacggaa
    10561 tctgtgggta actttgtatg tgtccgcagc gcccgccgca gtctcacgcc cggagcgtag
    10621 cgaccgagtg agcgaggagg cgcaatttcc ccggcacctg caagacggtc accggacgcg
    10681 ctgatgtggg atattgtccc ctgattaagt ctttctattc cctgacgatc aattatcagg
    10741 ataggtgaac catgtcggtt tttcgctgaa cattttacgt taacctatct ctgttgcttt
    10801 tttctccttg cggagaaatg caagtcatcc ccgctggggc gtggctgact tcaggggaaa
    10861 agaccttgct tacgctggct ggacgccacc gacgcacggt cttttcccct ctttctggct
    10921 ctgtccggct ggacgccggc cacgccagaa attcacccgg cccccgtcag gctcacgcct
    10981 gaccacccac aaaaatccac ggtgctggac gcacctgacg aagcagcaaa gctgctcctg
    11041 attttgcggg tggtcagccg ctcgctatgg cgaactctga cgggggccgg aacaccggaa
    11101 aagaccgcac tggggtgccg ccttttcctc tcacacgctg gggcgtgctc gttgttcctc
    11161 cggggctgcg ccttttccgc atggggcgga aagaggctgg cctccgcggt gaaagctgaa
    11221 cgcaatcacc acggacgtga ccgccgccgc catgccttgg ggcatgtccg cggcttgtgc
    11281 agcgggggct gcgcatcacg ccggtaaacc cgtcgtctga aggagagaga ttgcatgttt
    11341 aatacggatt cgcttgcctg gcagtggcat atcccggagc tggcccggca gatttacccg
    11401 tgggtcagct ggctgtaccg ggagtattac agtctggccg gtttttttct ggttatcggc
    11461 ctgtttttcg gtctggctga actctgggcc ggccgaatgc gttttgtggg gctgagccat
    11521 aaggcgcggc gggagtatgc cgatcgctat aagcgcgata ataaccggac tgtgccattt
    11581 tcggtgctgg cctcgctgga ttcacccggg cggatggtca gggcacggcg acatgtctgg
    11641 aactacgcgt actggctgac cggcgctgcc ggtctggccc ttctgggggg gctggttgtc
    11701 gccgtgttta tcgagagctg gggagaaacc gtcctgcgcc cggacctcta tgcgaacgca
    11761 gattattacc tgctgcagcc ggctaacctg ttcgggatag cctggtgtgc cggagggctt
    11821 gtcgccggcg tccttctggc gcggctgctg gcgttcctgc tgcttgatgg tcattttgcg
    11881 gccgctgacg aggcggtgaa cgcgaaactg aatcaggaga gccggcgcag cagccagcgg
    11941 accggagaaa tgacagacgt ccgccacctg
//
