LOCUS       NZ_CP087650             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01754 plasmid
            pDD01754-5, complete sequence.
ACCESSION   NZ_CP087650
VERSION     NZ_CP087650.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822722
            Assembly: GCF_023066745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066745.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:24:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,065
            CDSs (total)                      :: 5,943
            Genes (coding)                    :: 5,745
            CDSs (with protein)               :: 5,745
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 62 of 198
            Pseudo Genes (incomplete)         :: 144 of 198
            Pseudo Genes (internal stop)      :: 22 of 198
            Pseudo Genes (multiple problems)  :: 26 of 198
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087650.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01754"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01754-5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            join(5510..5596,1..423)
                     /locus_tag="LMH55_RS30165"
                     /old_locus_tag="LMH55_30165"
     CDS             join(5510..5596,1..423)
                     /locus_tag="LMH55_RS30165"
                     /old_locus_tag="LMH55_30165"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            430..642
                     /locus_tag="LMH55_RS30170"
                     /old_locus_tag="LMH55_30170"
     CDS             430..642
                     /locus_tag="LMH55_RS30170"
                     /old_locus_tag="LMH55_30170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1309..1533
                     /locus_tag="LMH55_RS30175"
                     /old_locus_tag="LMH55_30175"
     CDS             1309..1533
                     /locus_tag="LMH55_RS30175"
                     /old_locus_tag="LMH55_30175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1517..1783
                     /locus_tag="LMH55_RS30180"
                     /old_locus_tag="LMH55_30180"
     CDS             1517..1783
                     /locus_tag="LMH55_RS30180"
                     /old_locus_tag="LMH55_30180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1853..2203)
                     /locus_tag="LMH55_RS30185"
                     /old_locus_tag="LMH55_30185"
     CDS             complement(1853..2203)
                     /locus_tag="LMH55_RS30185"
                     /old_locus_tag="LMH55_30185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2269..2814)
                     /locus_tag="LMH55_RS30190"
                     /old_locus_tag="LMH55_30190"
     CDS             complement(2269..2814)
                     /locus_tag="LMH55_RS30190"
                     /old_locus_tag="LMH55_30190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2840..3166)
                     /locus_tag="LMH55_RS30195"
                     /old_locus_tag="LMH55_30195"
     CDS             complement(2840..3166)
                     /locus_tag="LMH55_RS30195"
                     /old_locus_tag="LMH55_30195"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3524..3682)
                     /locus_tag="LMH55_RS30400"
     CDS             complement(3524..3682)
                     /locus_tag="LMH55_RS30400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4322..4498
                     /locus_tag="LMH55_RS30200"
                     /old_locus_tag="LMH55_30200"
     CDS             4322..4498
                     /locus_tag="LMH55_RS30200"
                     /old_locus_tag="LMH55_30200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4488..4832
                     /locus_tag="LMH55_RS30205"
                     /old_locus_tag="LMH55_30205"
     CDS             4488..4832
                     /locus_tag="LMH55_RS30205"
                     /old_locus_tag="LMH55_30205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5014..5259)
                     /locus_tag="LMH55_RS30210"
                     /old_locus_tag="LMH55_30210"
     CDS             complement(5014..5259)
                     /locus_tag="LMH55_RS30210"
                     /old_locus_tag="LMH55_30210"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4157..4236
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3574..3971)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          4..758
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.603"
                     /coverage="99.0"
                     /evalue="6.19e-139"
     Typing          4823..5595
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="2.19e-168"
ORIGIN
        1 ttcagcgagc acgagaagtc tgtcagagcg gaactgagcg aaagcgggaa gagaatcagc
       61 gccgccatcc gcgcccacga gcagggtatg agcgcggcca tgcagtcgaa ccgcctgagc
      121 gtgatgcgta tggtcgggcg gacatggctg accatcacga tggtgtcagg actgctgttc
      181 gccagcctga gcggggtgtt gtggtatcag gggagcttga tagcgtcaaa tctggccgaa
      241 atcgaccggc agaacgcagc gctgtcgaag ctgaacgcga agacctgggg cgtgacgtac
      301 ctggaagaca gcaacggacg ttttctggtg ctgccgaagg ggacggccgt agacaggacg
      361 cagagctgga cggtcgggaa cgggcggtcg aagcagaacg cgctgagact ggtgaaggag
      421 taagtgccga tgaccgagct ggaaaaacag ttgctgagcg cattagagca gctacagcag
      481 gactactcga aaaggctgga cgagtgggag agcgccttcg gggaatggcg gaaaatgtca
      541 gggcttatgc aacgggagaa cgcggcgctg agcgagcgcg tgacgcgctt gagtcagcag
      601 gtggaacgct tgagcgggca actgcagcgt ttgagccggt agtgcagcgt catgagatgg
      661 ccgtggccgc agaacgggcg cacgagcaac ggcagcacga aaaggaactg acggaagcgc
      721 gttcccggca gcgaagctat gacggtccgt cgctgggctg aaatgcagga aaacagggtt
      781 ctcatcgggc atttacgctg aaaccggatg tcattcacct ggctgcatgg ctatgcagcc
      841 aggtaaaatt tatctgtcgc gaacggggcg gtttatcggg tggtttgttg ccggttttac
      901 ctgactgcca ccccgttcgc ggcgaacccg tccggggcgg tgcgggcaac gggtgttatg
      961 tttaatcagc gtgaacggca gatacaaaaa agccccggag ggcttttaac tactgactta
     1021 tttttttgaa gatttgctgg gtttcaattt gtcagtattt cccggcgttt ttttattgtc
     1081 acgctggcgt ttatcaggtt cccctgttga atcggcaatt cgtttcggac ccggcttaag
     1141 acccataatc gttatccttt caatagttaa gaggaaagcc ctcaagctag atatattgac
     1201 cgcgttacgg attatcaaga ggggctaaca agattatttt ttgttctttt aggttgattg
     1261 tggtactgtt gatatacatg tatagcaaat ggtgttcggg aggtttatat gcttgctatc
     1321 cggttatctg atgagattga gtcccgtctg gactcgctgg cgaagcaaac cggcagaaca
     1381 aagacgtttt atgcgcggga agcaatactg gcgcatctgg aagacctgga ggattattat
     1441 ctttcagcag aaactgctgc acgcgttcgc cgtggtgatg aagcagtgca ttcgtctgaa
     1501 gacgtgagga agtcacttgg tctggacgat taactattcc gatcgggcgc tcaaatcgtt
     1561 acgcaagatg gacaaacaga acgcacgacg gattgtggat tttatgagtt tacgcattgc
     1621 agttgctgcc gatcctcgcc agtcagggaa gccgctcaaa ggtgagctgg gcgagttctg
     1681 gcgctatcgg gtgggagatt atcgcgttct gtgtgagatc cgagatgacg agcttgttat
     1741 ccttgccgcc acgattggac atcgccgcga agtttacgac tgaagcccgc tatgcgggct
     1801 tttttgatgc cgacaacagg tattatggta aatactcgac taactacgtg ttctactgtt
     1861 tgtcggtttt agttaaccag caggttgtct taacgataat gtagccgtta tttttaacta
     1921 ataaggtcat attgttaacg tcaggcataa taaaagacat tatggcctca gagttatcat
     1981 cagaagcagc tctctgcatt actacttcga ttcccttttc tttgaatcta taagtatcca
     2041 gttgcctatt aaacttagaa agacctacaa ccatacttag tttgttatct ttaacagtaa
     2101 gagatgcttc ctgagtacca ttacatcggt agtaagcggt gtaaccgtca gcaaaagatc
     2161 gaccggggaa tataaatgct aggaacgcaa ttaaccaata catagttgag aatcttggta
     2221 ttacacattt cactatcctc tctttcattt ttcgttcacc atactttgtt atctgatttt
     2281 taatttttct attttataaa ccaagtctct accaaaaagg cgaaaattat cctcaaataa
     2341 taataattgt ttaagaacat ccatgaaagc agctgcttca ccctcagtta tgggagctac
     2401 tctatataac atataacctg agttaagggt ttcttttata ctttcttcaa tgctcattat
     2461 aaatattagt ggattatcat ttttcttccc ccatgggttt ctaattaatc cttcgatgta
     2521 ctcaggtcct ttgcgtccta ttgcctgcgc aatatttttt atttcatctg cgtgagggct
     2581 ttgttgtaca gctttgttta tgcctgcttg agtgattttc agagactcta caccgtgtat
     2641 gttgtatgag ataaaatgta ataaattaag atgcaatgca attaatccat ttttgtttac
     2701 atctgagtat ttggataaaa aacgtaatga agtgttaact ctcccttgaa gcctaacaat
     2761 tgggagtgct gccttgtata tttccttctc cgttaaatac cgtttaaaaa acataattct
     2821 ccgcctccgt atagcgtgcc tattttattt cgtcgtaaaa cttagctctg aaatattcct
     2881 ttagaaccat catgtcatta ataattgaat ctatctcatc cattttttct tggtattctt
     2941 ttaatggtga agggagcggt aactcaatat ttgaaaagtg attggcgaga tagggatcaa
     3001 ggtttctcat ccattcaggc ttttcgccaa tgcgttcttc aactgaaagt gaacctgata
     3061 atctctggtt aataagatca cttagactca ttttggtttt attcgaaagc cgttgtaagg
     3121 attgctttaa tcctatatct agcatgatat ttgttctttc tctcattacc cccccttgct
     3181 attgcgtttt ttgttgcgca caaagctacc attgcgcatt aagaaacgca ataaaaaagg
     3241 agggagggag tggtttgtac atgatgtgca gtgcgctgag ggcatcagtg gcgggtgttt
     3301 ttccgtggga tggatggagc tccggggata acagaactga cgatcattta aaggatcttc
     3361 ttgagatcct ttttttctgc gcgtaatctc ttgcactgta aacgaaaaaa ccgcctgggg
     3421 aggcggtttg atcgaaggtt aagtcagttg gggaactgct taaccgggta actgggtata
     3481 cgagaccgca gataccaaaa ctgttctttc agtgtagccg cagttaggca cccacttcaa
     3541 gacctctcaa tatctctctc gtatatatcc agttaccaat ggctgctgcc agtggcgttt
     3601 ttgcgtgtcc gtccgggttg gactcaagat gatagttacc ggaaagggcg cagcagtcgg
     3661 gctgaacggg gggttcgtgc atacagccca gcttggagcg aacgacctaa accgagccga
     3721 gataccagca gcgtgagcta tgagaaagcg ccacgcttcc cgaaagggaa aaaggcggaa
     3781 caggtatccg gtaaacggca gggtcggaac aggagagcgc acgagggagc cgccggagag
     3841 aaatcaccgg catctttaag tcctgtcggg tttcgtcctc ctctgacttg agcgtccatt
     3901 tctgtgatgc tcgtcagggg aggcggagcc tatggaaaaa cggcaacgcc gcatcctttt
     3961 ctccggccct ttctgatttg agcgcctttg ctttatcccg ttatccgtga gtcggtcacg
     4021 cccgccgcag ccgaacggca ggagcgcagc taccgagtga gcgaggaggc gtcatttcat
     4081 ctgtccgtgc atggtattgc ggacgctcct gagccacata tcacacctgc cacatgaggc
     4141 actttcatga atcgtgctct catgccaaca tagtaagcca gtatacacag cgtaaacgct
     4201 gtgactggtt cagggctgcg ccccgaaacc cgctaacacc actgacgcgc tggcgcttgt
     4261 ccaacgccgg agcgacccgg aaaacttttc cgtgccgtcc ggcgtttgcg aaggagggat
     4321 aatgcagaag ccgaagaaga agccgctcgt cgtaaaaaaa agatcctggc atccggcagt
     4381 gatggcaaat ctgatgcgtc aggcacagga ggaagattac cgccgggagc agatggcaga
     4441 gcttgagcgt tcagcccggt cacattttgc ggatatcgga ggagaggatg gcggttaagc
     4501 gtacccgttt tttaggtatc cgggtgaccg atggggagta ccagcagctg ctggagcgct
     4561 gcaatggaag gcaactggcg gtgtggatgc gggaaacctg tctcgatacg cgcccggcgc
     4621 ggtcgttgcg gctgccgtcc atcgatccgg ttttgctgcg ccagcttgcc gggatgggga
     4681 acaacctcaa ccagatcgcc cggaaaatta acggcggcca gtggtcaggc gctgaccggg
     4741 ttcaggtggt ggccgcgctg atggccattg atgccgaact cgagcggctg cggcatgccg
     4801 tgctggaaaa gggggcagac gatgatcgtt aagtttcatc cgcgagggcg aggcggcggt
     4861 gccgggccgg tggattatct gctggggaaa gaccgccagc gcgacggggc catcgtcctg
     4921 cagggaaagc cggaggaggt ccgggagctt atcgacgcct cgccctacgc caaaaagtac
     4981 acctccggcg tcctgtcttt tgctgaacag gatttaccgc ccggccagcg tgaaaagctg
     5041 atggcgagct tcgagcgggt tctgatgccc ggactcgata aagaccagta cagcgtgctg
     5101 tgggttgaac accgggacaa ggggcggctg gaactgaatt ttctggtacc gaacacggaa
     5161 ctgctgaccg gcaggcgtct ccagccgtac tacgaccggg cagaccgtcc gcgcatcgat
     5221 gcgtggcaga ccgtggtgaa cgggcgactg gggctgcacg acccgaacgc accggagaac
     5281 cggcgcgcgc tggtgacgcc atcttcgtta cccaaaacga agcaggaagc cgcagaggct
     5341 attacgcggg gcttactggc cctcgcctcg tcaggggagc ttaaaacgcg tcaggacgtc
     5401 actgaggcgc tggaaagcgc aggttttgag gtcgtgcgca ccacaaagag cagcatcagc
     5461 attgccgacc cggacggagg gcgaaacatc cgacttaagg gagccatcta tgaacagtct
     5521 tttaacgctg gcgaaggact tagagcagaa atcgaaagcg cagcagcaga gtaccggcga
     5581 aatgctgaaa gccgca
//
