LOCUS       NZ_CP087649            10061 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01754 plasmid
            pDD01754-4, complete sequence.
ACCESSION   NZ_CP087649
VERSION     NZ_CP087649.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822722
            Assembly: GCF_023066745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10061)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066745.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:24:57
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,065
            CDSs (total)                      :: 5,943
            Genes (coding)                    :: 5,745
            CDSs (with protein)               :: 5,745
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 198
            CDSs (without protein)            :: 198
            Pseudo Genes (ambiguous residues) :: 0 of 198
            Pseudo Genes (frameshifted)       :: 62 of 198
            Pseudo Genes (incomplete)         :: 144 of 198
            Pseudo Genes (internal stop)      :: 22 of 198
            Pseudo Genes (multiple problems)  :: 26 of 198
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087649.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01754"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01754-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            join(9981..10061,1..177)
                     /locus_tag="LMH55_RS30100"
                     /old_locus_tag="LMH55_30100"
     CDS             join(9981..10061,1..177)
                     /locus_tag="LMH55_RS30100"
                     /old_locus_tag="LMH55_30100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            262..411
                     /locus_tag="LMH55_RS30105"
                     /old_locus_tag="LMH55_30105"
     CDS             262..411
                     /locus_tag="LMH55_RS30105"
                     /old_locus_tag="LMH55_30105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(626..775)
                     /locus_tag="LMH55_RS30395"
     CDS             complement(626..775)
                     /locus_tag="LMH55_RS30395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_320563032.1"
                     /translation="MGCKQELPVQPDCCALSGNCHLESNPDEHAKTPLAAAIGNWEFAE
                     DLFS"
                     /name="replication initiation protein"
     gene            1896..3860
                     /locus_tag="LMH55_RS30110"
                     /old_locus_tag="LMH55_30110"
     CDS             1896..3860
                     /locus_tag="LMH55_RS30110"
                     /old_locus_tag="LMH55_30110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3860..4591
                     /gene="mobC"
                     /locus_tag="LMH55_RS30115"
                     /old_locus_tag="LMH55_30115"
     CDS             3860..4591
                     /gene="mobC"
                     /locus_tag="LMH55_RS30115"
                     /old_locus_tag="LMH55_30115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            4598..5128
                     /locus_tag="LMH55_RS30120"
                     /old_locus_tag="LMH55_30120"
     CDS             4598..5128
                     /locus_tag="LMH55_RS30120"
                     /old_locus_tag="LMH55_30120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(5155..5334)
                     /locus_tag="LMH55_RS30125"
                     /old_locus_tag="LMH55_30125"
     CDS             complement(5155..5334)
                     /locus_tag="LMH55_RS30125"
                     /old_locus_tag="LMH55_30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(5360..5788)
                     /locus_tag="LMH55_RS30130"
                     /old_locus_tag="LMH55_30130"
     CDS             complement(5360..5788)
                     /locus_tag="LMH55_RS30130"
                     /old_locus_tag="LMH55_30130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            complement(5952..6266)
                     /locus_tag="LMH55_RS30135"
                     /old_locus_tag="LMH55_30135"
     CDS             complement(5952..6266)
                     /locus_tag="LMH55_RS30135"
                     /old_locus_tag="LMH55_30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(6254..6532)
                     /locus_tag="LMH55_RS30140"
                     /old_locus_tag="LMH55_30140"
     CDS             complement(6254..6532)
                     /locus_tag="LMH55_RS30140"
                     /old_locus_tag="LMH55_30140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            complement(6707..7072)
                     /locus_tag="LMH55_RS30145"
                     /old_locus_tag="LMH55_30145"
     CDS             complement(6707..7072)
                     /locus_tag="LMH55_RS30145"
                     /old_locus_tag="LMH55_30145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            complement(7069..7440)
                     /gene="tolA"
                     /locus_tag="LMH55_RS30150"
                     /old_locus_tag="LMH55_30150"
     CDS             complement(7069..7440)
                     /gene="tolA"
                     /locus_tag="LMH55_RS30150"
                     /old_locus_tag="LMH55_30150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            complement(7714..7959)
                     /locus_tag="LMH55_RS30155"
                     /old_locus_tag="LMH55_30155"
     CDS             complement(7714..7959)
                     /locus_tag="LMH55_RS30155"
                     /old_locus_tag="LMH55_30155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            8286..9971
                     /locus_tag="LMH55_RS30160"
                     /old_locus_tag="LMH55_30160"
     CDS             8286..9971
                     /locus_tag="LMH55_RS30160"
                     /old_locus_tag="LMH55_30160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     BGC             3286..10061
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(515..1094)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.599"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3861..4588
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gacgatggtt ctgtcattga aagcttggga atgcctttaa aggataatat taataatggt
       61 gagtttgatt tgaagaaaga atgggttcct ttactacagc cattttttaa aaatcaaata
      121 aaacctgatg tggataattg ttatatagct tttgattatc gggacggtaa ctggtaacaa
      181 aaaattatca aggatgattt gaaatactga cttccggtgt tcagggatga atgccggagt
      241 ctttaaatac agggaaagta tatgaaaaag gtaaagacta tttttctttt tatattgatt
      301 gttgctggct ttcttctggc cgcatgtcag gcaaattata tccgggatgt tcagggcgga
      361 acggtggcac catcgtcctc ctctgaactg acggggatcg cggttcagta gaaaagatca
      421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa
      481 ccacctgggg aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt
      541 aactggcttt cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc
      601 gcacacttca agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac
      661 caatggctgc tgccagtggc gtttttgcgt gttcgtccgg gttggactca agatgacagt
      721 taccggataa ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg
      781 agcgaacgac ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact
      841 tcccgcaagg gagaacggcg gacaggtatc cggtaaacgg cagggtcgga acaggagagc
      901 gcaagaggga gcgacctacc ggaaacggtg gggatcttta agtcctgtcg ggtttcgccc
      961 gtactgtcag attcatggtt gagcctcacg gctcccacag atgcaccgaa aaagcgtctg
     1021 tttatgtgaa ctctggcagg agggcggagc ctatggaaaa acgccaccgg cgcggccctg
     1081 ccgttttgcc tcacatgtta gtcccctgct tatccacgga atctgtgggt aactttgtat
     1141 gtgtccgcag cgcccgccgc agtctcacgc ccggagcgta gcgaccgagt gagcgaggag
     1201 gcgcaatttc cccggcacct gcaagacggt caccggacgc gctgatgtgg gatattgtcc
     1261 cctgattaag tctttctatt ccctgacgat caattatcag gataggtgaa ccatgtcggt
     1321 ttttcgctga acattttacg ttaacctatc tctgttgctt ttttctcctt gcggagaaat
     1381 gcaagtcatc cccgctgggg cgtggctgac ttcaggggaa aagaccttgc ttacgctggc
     1441 tggacgccac cgacgcacgg tcttttcccc tctttctggc tctgtccggc tggacgccgg
     1501 ccacgccaga aattcacccg gcccccgtca ggctcacgcc tgaccaccca caaaaatcca
     1561 cggtgctgga cgcacctgac gaagcagcaa agctgctcct gattttgcgg gtggtcagcc
     1621 gctcgctatg gcgaactctg acgggggccg gaacaccgga aaagaccgca ctggggtgcc
     1681 gccttttcct ctcacacgct ggggcgtgct cgttgttcct ccggggctgc gccttttccg
     1741 catggggcgg aaagaggctg gcctccgcgg tgaaagctga acgcaatcac cacggacgtg
     1801 accgccgccg ccatgccttg gggcatgtcc gcggcttgtg cagcgggggc tgcgcatcac
     1861 gccggtaaac ccgtcgtctg aaggagagag attgcatgtt taatacggat tcgcttgcct
     1921 ggcagtggca tatcccggag ctggcccggc agatttaccc gtgggtcagc tggctgtacc
     1981 gggagtatta cagtctggcc ggtttttttc tggttatcgg cctgtttttc ggtctggctg
     2041 aactctgggc cggccgaatg cgttttgtgg ggctgagcca taaggcgcgg cgggagtatg
     2101 ccgatcgcta taagcgcgat aataaccgga ctgtgccatt ttcggtgctg gcctcgctgg
     2161 attcacccgg gcggatggtc agggcacggc gacatgtctg gaactacgcg tactggctga
     2221 ccggcgctgc cggtctggcc cttctggggg ggctggttgt cgccgtgttt atcgagagct
     2281 ggggagaaac cgtcctgcgc ccggacctct atgcgaacgc agattattac ctgctgcagc
     2341 cggctaacct gttcgggata gcctggtgtg ccggagggct tgtcgccggc gtccttctgg
     2401 cgcggctgct ggcgttcctg ctgcttgatg gtcattttgc ggccgctgac gaggcggtga
     2461 acgcgaaact gaatcaggag agccggcgca gcagccagcg gaccggagaa atgacagacg
     2521 tccgccacct gcatttcggg gagccggtac cggttaacgc gctggccgat ttttccacgg
     2581 agcaggcccg gaaacagcag gcggtgtttc tgggtaagga tgagcagggc cagccggtgc
     2641 tggtccctcg tgatacctgg cgcaagacca atattcagat cctcggcctg cccgggagcg
     2701 gcaagagcgc gatggccacc aacgcgctca ttcgctgcgt acgcgatttc ggggacgctg
     2761 tggtgtactt cgaccccaaa ggggacgcgt gggcgccgca cgtcttccgg gcgcactgcc
     2821 cggacttcac cctgctggat ctgcggccgg ggaaaccggc gcagctgaat ctgttccgcg
     2881 atctcgacca gtacgccctg aagaatctgc tggttgccgg gtttaacctg agtgagaccg
     2941 gcgacgtggc ggaccactac cgcatcagcg agcagaaagc ggcgaagctg attgcggagc
     3001 agttcccgca gggggcaaac attcagcagg tgctggcggc ggcgtacgcg ttgccggagg
     3061 ccctgaaaaa ggacgtgaag gggctgatca ccaagctgga gaacgtggcc gacctgagcg
     3121 tcctgcagac ggacagcggt atcgatgtgg cggggataat caacggcggc ggctgcctgt
     3181 acgtcatcgg ctcgatggac gatgaggcgg tgatccgggt gcagaagatg ctgtttgccc
     3241 gctgcgcgca aatcatcatt gcgcgggatg agttccggag gtggccgcac gcgagcatca
     3301 tgctcgatga gattaaatat ctgctgtcga agtacgtgct gaacgcgctg gggacgctgc
     3361 gcagccggga ctgcaacctg ctgctcgcgc accagtcgct gggcgacttc ggtcagtgcg
     3421 ggcaggatct gcccgctgat tttgtgaaaa ccacggtact ggataacacg ccgatccgct
     3481 ggttctatcg cgcggccagc caggagtccg cgcagtgggc ggcgggccag accggggaga
     3541 tccgggtgga cgtggagcgc cgccgggcca gccgggaggc ggggaacgtg gagcacatca
     3601 gcggcgacag cttcatccag aaggaggcgc ggccgctgtt tgacgtgaac accctgcaac
     3661 acctgcctga cgggttcgcg gtgatgaccg ggctgggcgt ggcgcggctg gcgttcagca
     3721 gcccgctgcg ggttgagcgc cgggaaattt cgctgaaaac cttcccggtg ctggcgaaaa
     3781 cggacccgct ggcggagtac ctgcctccgg ccgacggcgg cggaccaaaa gcggatgatg
     3841 attttgcggg gctttactga tgacactgga gcagtacaac gtcagccacg cgaagcggca
     3901 ggcgcggaac gcggagaaaa cacggctgac cctgcgctgg ctgcgggaag agctgtgttc
     3961 cacggcggag ctggttgcgc gacggctggg gattgcggcg gtacagccgg tttaccgctt
     4021 cctggacagt ctggtggcga aagggctgct ggtccgggcg aaatacccgg ttgacggccg
     4081 tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg ttctcgttcg acgaggacga
     4141 gccgctgacg gacgttattc cgtttcagcc gtcaagggtg tcggcggcgc agctgccgca
     4201 ccggctggcc gtgcagtccc tgcgcctctc catggagggc cggggggcca ccggctggcg
     4261 gtacctgcac cgtatggccc tgaaagggat gaaagtgccg gacgcgctgg cggagctgga
     4321 cggcagaacg gtggcatttg aggtagagcg caccgtcaag agccgcaggc ggtatcagga
     4381 agtggtgtcc gggtatctgt tcaaccgccg ggcgaacggc atcgatgaaa tctggtacat
     4441 ctgccctgac cggccgacgc ttgtccgggt acagcgggcg attctggcgg tggatgaaat
     4501 cgtgaatcca cagaccggcg aggcccgcaa aacggtggag ctggaccggg agaggctgtt
     4561 cgcgtgcttt aaatttatga caacggagtg agggattatg gcgctgtaca tggtatttat
     4621 tgaggacgtg gaagacaaag acggcgagcg ctgcattatg ccaaccagcg agcatctgga
     4681 agacatggtg gtgctcgcct ggcagaccgg ggcgtatgtt ttctgttttg tcgaccgggc
     4741 ccagcatctg gcgatgatga aatccatcgg gatgtatgac gagcggctca acagcgacga
     4801 aaaagccacg tactttttca ccggtgaaaa tccgcaatgg gaacgggaca aacccacagg
     4861 aatgaagctg tgtgattacc tctatcgaaa ggtggagcgg ttcgccatcg agaacggtgt
     4921 gcaggtggtt gatgccggga agtgctcacc ggagcagatt aacgcgatga ttgaggtggt
     4981 cagcgagcgg cacggggaaa gcgcggtgaa cgagaagctg aaagcggagc tgatgcggga
     5041 atataacagc cgctatttta acgtgccggt gcctgaactg ccggaggaca aacccgcccg
     5101 ccggtggtgg ccgttcggca aaaaataacg ccagcaccgg catggccggt gctgttattc
     5161 ttccccgagc ttatcccgca tcacctgcca gagtgtttcc gcatcctcgt gaagccgttc
     5221 gcacatgtcg gccggggtat ccatgtccag ctcgttcagc ttttccagca acagcagcga
     5281 ctgatcctta atgaatttag ccatattgag ggcagttttt tcctgcttcg tcatgttctc
     5341 cattcccttc ggtaagttct cagctaaacc agccggacca gaagcgtttt ttcttctgtg
     5401 tcagttctgt tgtcaatctg tccacttcgg cctggagttt ccggcgctca tcatcgtgct
     5461 ggcggcgttc ttcttcccgg gatgttttat cttccagcat cagggtgaca gcctgccgga
     5521 gatcggccag ttccttttgc attgccagaa gctgttcctg tgggaaattt ttttgtgtat
     5581 tttcacggtg tgaaatgccg ggaatttgtt tctcagggat attcacattt ccgtatacac
     5641 ggatcagttc tgacacatcc acgacagggt tgtttttccc gtcacgtgac acggtaacct
     5701 ttccctgttt tacatgattg tatagcgttc ttctggtaat gcccgcggct tgtgctgcct
     5761 gtgaaaggtt gagcaatgtt ttcgccactg tatacccctg attttatcgg gtgtgaaagg
     5821 tgtgtaattt ctcacggtaa atgtatcgct gaattttacc cgtttttccg ttcaggatgt
     5881 ggttttctga cgcgctgcgc ttgtcaaacc ggcgacggcc acgcaatgaa ttgcgccccc
     5941 gtcatcggtt attagaggct gtctgccccc ggatacccgg attctttctg atgcctgacg
     6001 gtgacgatca caatctggtc catttcccgg tcgtggcggt agagcatcac atagccactg
     6061 tcgccaaacc cgatcaccag ttccttatat tccagcggca gaaacggcac cgggcggcca
     6121 atgtctggca atgttttcag ttgctggata gcccggacga tcacctcacc tccttttctg
     6181 gctgccagcc gatttttggt tttgagaaag tcctgaaggc gttgtaaatc gcgctgtgcc
     6241 agcgcggaaa tcgttacctg tggcatggcg gagcatcctg ctcgttctca gttccccagg
     6301 tacttatcca ggcttccgct tcttccgcag taaggtgcag acccgtttcc tgatagtgct
     6361 gccatgcggc ctgtccgtcc cgcaggtact ggctgcgctt ctcttcccgg tcgatgtatt
     6421 cggtgattgc ctccagcatt agcgcgtgcg ctgagcggtg ccggtcatcg gccagcgttt
     6481 tcagccggtc cttaagttct tcatcgagcc tgatggatgt tgctgatgac ataaaagccc
     6541 ctttgtagtc atatgtatta cacatgacta ctttaatccg tggcggcgct atttgccaga
     6601 aatacggctt ttgctgccca tttttcagct tcgcttccac taatcagggc tgctacaggc
     6661 tcactcatca tccaggacta ttgcagcctg cgggttaaag gacctgttac tggcggcaac
     6721 catcatcatt gagcttttgg gtggtgactg gtcgcggatc ctgtaagccg tcatttttca
     6781 gactgaattc aaaatttata acctgtcgca ttgccgggtg ccctgcggga tatagccagt
     6841 gcctcattgc cgtcagcgtt tcccgaaaaa aaaggtgaca gccggtaagc gacacgttgt
     6901 ccactcgtcc atcgtccaga atatcaaacg tggcggtcac ctgaccttcg atgcgttttg
     6961 ctgcggcctg taccgggtag gttggctgca gtgtggttac gggttttagt ggtatcgcat
     7021 gtaccggagt gaggaacatg gccgccagca ctgtcagagt aaaagctttc atggtctgaa
     7081 atccagaggg gcgtttttga atatttgcca ggtattttca tccggtgccg gcggaatttt
     7141 ggcgtgggta acggctgata ttgctgcttt acagagtttc ggatctcctc cttcagcaat
     7201 ggcgctgttc agagtgccat cacgttgtat ggatatccgc acggtgcaca tttttcccgc
     7261 ataggcctct ggttcaaaca tctgcatttc aacagcggtc ttgatggccg cagcgtagct
     7321 actgatttta gatgacttat ccagagtata aattttcggc ggctgggtgg tggaatgctt
     7381 acaggccgtc aggagtaagg ggctgcacaa tgccagcagg ataaagggta ttaaacgcat
     7441 cgggttgatg acctgtcagt aaaaaatgtt acatgtatat ttgtcactct ttacggccac
     7501 gttcatttac ataaacacgg cttatcgtat cgtttttaca ccaaacggcc cgccatgcag
     7561 ttgcgtgcat aggcatgcag cgggttacaa tttaccgggc gcgtttccgg cggttttccg
     7621 ggtggtttgt tgcctgtttt atcccgtagc cgccggaaac gccctcagac cgtctgagcg
     7681 gtgcgggcaa tgcgacgtta tggtaaatac cccttaccgg cggcgctgtg gctgccaggt
     7741 gaaggtggtt tccgtagcct cgtatttctc ccgacgcatt tcctgcgccc gcctggcttc
     7801 acgttcgtcc tgaatggcac gaagcaccct ttcccgcacc tgttgtttta ccgcctttgg
     7861 cagcgttttg ttccctttca ggcccatggc cagtgcctga gcgacaaagg cgtccgccag
     7921 gcgcttttct tcagcgggag tcagtttcag gtgcgtcata gtggcctccg ggcgtctcag
     7981 gtttcccgaa cagtttaacc gcaggtcctc tcctcatgcc agttagcttt cagatgtgtc
     8041 tataccatct gaaatgtaag tgtgatgaat gtcttaaata ataaaatttg ttgccatgag
     8101 aaattttcgg gaaaagacgc gatctgctta caggtggctc cgggcgcttt tttgtgcctg
     8161 aagatgagaa ctaatctcat atattgacac ctgaaaacgg gaggagtaag gtaataatta
     8221 tactgtgtat atgtacagta tgttggcttt aatttattgt tttaaatgtc aaagaggaaa
     8281 acggtatgag tggtggagac ggtcgaggtc cgggtaattc aggtctggga cataatggtg
     8341 gtcaggccag tgggaatgtg aacggtacgt ctggtaaagg tggcccttca tcaggtggtg
     8401 gtacggatcc aaacagcggg ccgggctggg gtacgacgca tacgccgaat ggagatattc
     8461 ataactataa tccgggggag tttggtaacg gcgggagtaa acccggtggt aatggtggta
     8521 acagcggcaa tcatagcggt agctctggtg gcggacagtc ttcggccact gcgatggcct
     8581 tcggtctgcc tgccctggct actccggggg ctgaaggact ggctttatca gtttccggcg
     8641 atgcgttgtc ggccgccgtt gctgatgtgc tggctgccct gaaggggccg tttaagtttg
     8701 gtctgtgggg gattgcgatc tacggtgtgc tgccttctga gattgcaaaa gatgatccga
     8761 aaatgatgtc aaaaattatg acgtcattac cggccgatgc ggtgacggag actccggcaa
     8821 gtactttacc actggaccag gcgacggttc gtgtcagaca acgggttgtg gatgtggtga
     8881 aggatgagcg gcagcatatt gcggttgtcg caggtcggcc aatgagtgtc cctgtggtgg
     8941 atgcgaaacc gacaaaacgt ccgggggtat tcagtgtgtc gattccgggt ctcccgtctc
     9001 tgcaggtgag cgtaccgaaa ggtgttccga cagcgaaagc cccgccaaaa ggcattgttg
     9061 ctgaaaaagg tgattcacgt ccggctggtt ttacagccgg tggtaactcc cgtgaggccg
     9121 ttattcgttt cccgaaagag accggacaga agccggttta tgtgtcggtg acagatgttc
     9181 ttaccccggc acaggtaaaa cagcgtcagg aggaagaaaa gcgtcgccag caggcatggg
     9241 acgccgctca tccggaagag gggctgaaaa gagactatga taaagcgaaa gctgagctgg
     9301 atgccgaaga taaaaatatt gcgaccttaa acagccgcat tgcatcgaca gagaaggcgc
     9361 tccccggtgc aagggctgct gttcaggaag ccgataaaaa ggtaaaagag gcagaggcaa
     9421 ataaagatga ttttgtgact tataacccgc ctcatgaata tggctccggg tggcaggatc
     9481 aggttcgcta tcttgataag gatattcaga atcagaatga gaaattaaaa gcggctcagg
     9541 catctttaaa cgcaatgaat gaatccttat ccagagataa ggctgcactt accggggcga
     9601 tggagagccg gaaacaaaag gagaaaaaag cgaaggatgc agaaaataag ttaaatgagg
     9661 aaaagaacaa gcctcgcaag gggactaaag actacggcca tgattatcat ccagtaccta
     9721 aaacagaaga cataaaggga cttggagagt taaagcgagg tgatcctaaa acacctaaac
     9781 aaggtggtgg tggtaagcgg gcccgatggt atggcgataa aaaacgtaaa atttatgaat
     9841 gggattccca gcacggtgag cttgaagggt atcgtgccag tgatggcgaa cacctaggcg
     9901 catttgatcc caaaacgggt aagcaggtta aagggccaga tccaaaacgg aacattaaaa
     9961 aatatctttg agaggtaaat atggggctta aattacgttt gacatggttt gataagaaaa
    10021 ctgaagagtt taaaggggaa gagtattcta aagactttgg t
//
