LOCUS       NZ_CP087644             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02391 plasmid
            pDD02391-5, complete sequence.
ACCESSION   NZ_CP087644
VERSION     NZ_CP087644.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822723
            Assembly: GCF_023066725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066725.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:10:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,878
            CDSs (total)                      :: 5,756
            Genes (coding)                    :: 5,584
            CDSs (with protein)               :: 5,584
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 60 of 172
            Pseudo Genes (incomplete)         :: 113 of 172
            Pseudo Genes (internal stop)      :: 23 of 172
            Pseudo Genes (multiple problems)  :: 24 of 172
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087644.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02391"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02391-5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(join(5478..5596,1..58))
                     /locus_tag="LMH57_RS29230"
                     /old_locus_tag="LMH57_29230"
     CDS             complement(join(5478..5596,1..58))
                     /locus_tag="LMH57_RS29230"
                     /old_locus_tag="LMH57_29230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            698..856
                     /locus_tag="LMH57_RS29435"
     CDS             698..856
                     /locus_tag="LMH57_RS29435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1214..1540
                     /locus_tag="LMH57_RS29235"
                     /old_locus_tag="LMH57_29235"
     CDS             1214..1540
                     /locus_tag="LMH57_RS29235"
                     /old_locus_tag="LMH57_29235"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1566..2111
                     /locus_tag="LMH57_RS29240"
                     /old_locus_tag="LMH57_29240"
     CDS             1566..2111
                     /locus_tag="LMH57_RS29240"
                     /old_locus_tag="LMH57_29240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2177..2527
                     /locus_tag="LMH57_RS29245"
                     /old_locus_tag="LMH57_29245"
     CDS             2177..2527
                     /locus_tag="LMH57_RS29245"
                     /old_locus_tag="LMH57_29245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2597..2863)
                     /locus_tag="LMH57_RS29250"
                     /old_locus_tag="LMH57_29250"
     CDS             complement(2597..2863)
                     /locus_tag="LMH57_RS29250"
                     /old_locus_tag="LMH57_29250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2847..3071)
                     /locus_tag="LMH57_RS29255"
                     /old_locus_tag="LMH57_29255"
     CDS             complement(2847..3071)
                     /locus_tag="LMH57_RS29255"
                     /old_locus_tag="LMH57_29255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3738..3950)
                     /locus_tag="LMH57_RS29260"
                     /old_locus_tag="LMH57_29260"
     CDS             complement(3738..3950)
                     /locus_tag="LMH57_RS29260"
                     /old_locus_tag="LMH57_29260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3957..4466)
                     /locus_tag="LMH57_RS29265"
                     /old_locus_tag="LMH57_29265"
     CDS             complement(3957..4466)
                     /locus_tag="LMH57_RS29265"
                     /old_locus_tag="LMH57_29265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4501..5205)
                     /locus_tag="LMH57_RS29440"
                     /pseudo=""
     CDS             complement(<4501..5205)
                     /locus_tag="LMH57_RS29440"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5144..5488)
                     /locus_tag="LMH57_RS29275"
                     /old_locus_tag="LMH57_29275"
     CDS             complement(5144..5488)
                     /locus_tag="LMH57_RS29275"
                     /old_locus_tag="LMH57_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(145..221)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          410..807
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3623..5154)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctgccggatg ccaggatctt ttttttacga cgagcggctt cttcttcggc ttctgcatta
       61 tccctccttc gcaaacgccg gacggcacgg aaaagttttc cgggtcgctc cggcgttgga
      121 caagcgccag cgcgtcagtg gtgttagcgg gtttcggggc gcagccctga accagtcaca
      181 gcgtttacgc tgtgtatact ggcttactat gttggcatga gagcacgatt catgaaagtg
      241 cctcatgtgg caggtgtgat atgtggctca ggagcgtccg caataccatg cacggacaga
      301 tgaaatgacg cctcctcgct cactcggtag ctgcgctcct gccgttcggc tgcggcgggc
      361 gtgaccgact cacggataac gggataaagc aaaggcgctc aaatcagaaa gggccggaga
      421 aaaggatgcg gcgttgccgt ttttccatag gctccgcctc ccctgacgag catcacagaa
      481 atggacgctc aagtcagagg aggacgaaac ccgacaggac ttaaagatgc cggtgatttc
      541 tctccggcgg ctccctcgtg cgctctcctg ttccgaccct gccgtttacc ggatacctgt
      601 tccgcctttt tccctttcgg gaagcgtggc gctttctcat agctcacgct gctggtatct
      661 cggctcggtt taggtcgttc gctccaagct gggctgtatg cacgaacccc ccgttcagcc
      721 cgactgctgc gccctttccg gtaactatca tcttgagtcc aacccggacg gacacgcaaa
      781 aacgccactg gcagcagcca ttggtaactg gatatatacg agagagatat tgagaggtct
      841 tgaagtgggt gcctaactgc ggctacactg aaagaacagt tttggtatct gcggtctcgt
      901 atacccagtt acccggttaa gcagttcccc aactgactta accttcgatc aaaccgcctc
      961 cccaggcggt tttttcgttt acagtgcaag agattacgcg cagaaaaaaa ggatctcaag
     1021 aagatccttt aaatgatcgt cagttctgtt atccccggag ctccatccat cccacggaaa
     1081 aacacccgcc actgatgccc tcagcgcact gcacatcatg tacaaaccac tccctccctc
     1141 cttttttatt gcgtttctta atgcgcaatg gtagctttgt gcgcaacaaa aaacgcaata
     1201 gcaagggggg gtaatgagag aaagaacaaa tatcatgcta gatataggat taaagcaatc
     1261 cttacaacgg ctttcgaata aaaccaaaat gagtctaagt gatcttatta accagagatt
     1321 atcaggttca ctttcagttg aagaacgcat tggcgaaaag cctgaatgga tgagaaacct
     1381 tgatccctat ctcgccaatc acttttcaaa tattgagtta ccgctccctt caccattaaa
     1441 agaataccaa gaaaaaatgg atgagataga ttcaattatt aatgacatga tggttctaaa
     1501 ggaatatttc agagctaagt tttacgacga aataaaatag gcacgctata cggaggcgga
     1561 gaattatgtt ttttaaacgg tatttaacgg agaaggaaat atacaaggca gcactcccaa
     1621 ttgttaggct tcaagggaga gttaacactt cattacgttt tttatccaaa tactcagatg
     1681 taaacaaaaa tggattaatt gcattgcatc ttaatttatt acattttatc tcatacaaca
     1741 tacacggtgt agagtctctg aaaatcactc aagcaggcat aaacaaagct gtacaacaaa
     1801 gccctcacgc agatgaaata aaaaatattg cgcaggcaat aggacgcaaa ggacctgagt
     1861 acatcgaagg attaattaga aacccatggg ggaagaaaaa tgataatcca ctaatattta
     1921 taatgagcat tgaagaaagt ataaaagaaa cccttaactc aggttatatg ttatatagag
     1981 tagctcccat aactgagggt gaagcagctg ctttcatgga tgttcttaaa caattattat
     2041 tatttgagga taattttcgc ctttttggta gagacttggt ttataaaata gaaaaattaa
     2101 aaatcagata acaaagtatg gtgaacgaaa aatgaaagag aggatagtga aatgtgtaat
     2161 accaagattc tcaactatgt attggttaat tgcgttccta gcatttatat tccccggtcg
     2221 atcttttgct gacggttaca ccgcttacta ccgatgtaat ggtactcagg aagcatctct
     2281 tactgttaaa gataacaaac taagtatggt tgtaggtctt tctaagttta ataggcaact
     2341 ggatacttat agattcaaag aaaagggaat cgaagtagta atgcagagag ctgcttctga
     2401 tgataactct gaggccataa tgtcttttat tatgcctgac gttaacaata tgaccttatt
     2461 agttaaaaat aacggctaca ttatcgttaa gacaacctgc tggttaacta aaaccgacaa
     2521 acagtagaac acgtagttag tcgagtattt accataatac ctgttgtcgg catcaaaaaa
     2581 gcccgcatag cgggcttcag tcgtaaactt cgcggcgatg tccaatcgtg gcggcaagga
     2641 taacaagctc gtcatctcgg atctcacaca gaacgcgata atctcccacc cgatagcgcc
     2701 agaactcgcc cagctcacct ttgagcggct tccctgactg gcgaggatcg gcagcaactg
     2761 caatgcgtaa actcataaaa tccacaatcc gtcgtgcgtt ctgtttgtcc atcttgcgta
     2821 acgatttgag cgcccgatcg gaatagttaa tcgtccagac caagtgactt cctcacgtct
     2881 tcagacgaat gcactgcttc atcaccacgg cgaacgcgtg cagcagtttc tgctgaaaga
     2941 taataatcct ccaggtcttc cagatgcgcc agtattgctt cccgcgcata aaacgtcttt
     3001 gttctgccgg tttgcttcgc cagcgagtcc agacgggact caatctcatc agataaccgg
     3061 atagcaagca tataaacctc ccgaacacca tttgctatac atgtatatca acagtaccac
     3121 aatcaaccta aaagaacaaa aaataatctt gttagcccct cttgataatc cgtaacgcgg
     3181 tcaatatatc tagcttgagg gctttcctct taactattga aaggataacg attatgggtc
     3241 ttaagccggg tccgaaacga attgccgatt caacagggga acctgataaa cgccagcgtg
     3301 acaataaaaa aacgccggga aatactgaca aattgaaacc cagcaaatct tcaaaaaaat
     3361 aagtcagtag ttaaaagccc tccggggctt ttttgtatct gccgttcacg ctgattaaac
     3421 ataacacccg ttgcccgcac cgccccggac gggttcgccg cgaacggggt ggcagtcagg
     3481 taaaaccggc aacaaaccac ccgataaacc gccccgttcg cgacagataa attttacctg
     3541 gctgcatagc catgcagcca ggtgaatgac atccggtttc agcgtaaatg cccgatgaga
     3601 accctgtttt cctgcatttc agcccagcga cggaccgtca tagcttcgct gccgggaacg
     3661 cgcttccgtc agttcctttt cgtgctgccg ttgctcgtgc gcccgttctg cggccacggc
     3721 catctcatga cgctgcacta ccggctcaaa cgctgcagtt gcccgctcaa gcgttccacc
     3781 tgctgactca agcgcgtcac gcgctcgctc agcgccgcgt tctcccgttg cataagccct
     3841 gacattttcc gccattcccc gaaggcgctc tcccactcgt ccagcctttt cgagtagtcc
     3901 tgctgtagct gctctaatgc gctcagcaac tgtttttcca gctcggtcat cggcacttac
     3961 tccttcacca gtctcagcgc gttctgcttc gaccgcccgt tcccgaccgt ccagctctgc
     4021 gtcctgtcta cggccgtccc cttcggcagc accagaaaac gtccgttgct gtcttccagg
     4081 tacgtcacgc cccaggtctt cgcgttcagc ttcgacagcg ctgcgttctg ccggtcgatt
     4141 tcggccagat ttgacgctat caagctcccc tgataccaca acaccccgct caggctggcg
     4201 aacagcagtc ctgacaccat cgtgatggtc agccatgtcc gcccgaccat acgcatcacg
     4261 ctcaggcggt tcgactgcat ggccgcgctc ataccctgct cgtgggcgcg gatggcggcg
     4321 ctgattctct tcccgctttc gctcagttcc gctctgacag acttctcgtg ctcgctgaat
     4381 gcggctttca gcatttcgcc ggtactctgc tgctgcgctt tcgatttctg ctctaagtcc
     4441 ttcgccagcg ttaaaagact gttcatagat ggctccctta agtcggatgt ttcgccctcc
     4501 gtccgggtcg gcaatgctga tgctgctctt tgtggtgcgc acgacctcaa aacctgcgct
     4561 ttccagcgcc tcagtgacgt cctgacgcgt tttaagctcc cctgacgagg cgagggccag
     4621 taagccccgc gtaatagcct ctgcggcttc ctgcttcgtt ttgggtaacg aagatggcgt
     4681 caccagcgcg cgccggttct ccggtgcgtt cgggtcgtgc agccccagtc gcccgttcac
     4741 cacggtctgc cacgcatcga tgcgcggacg gtctgcccgg tcgtagtacg gctggagacg
     4801 cctgccggtc agcagttccg tgttcggtac cagaaaattc agttccagcc gccccttgtc
     4861 ccggtgttca acccacagca cgctgtactg gtctttatcg agtccgggca tcagaacccg
     4921 ctcgaagctc gccatcagct tttcacgctg gccgggcggt aaatcctgtt cagcaaaaga
     4981 caggacgccg gaggtgtact ttttggcgta gggcgaggcg tcgataagct cccggacctc
     5041 ctccggcttt ccctgcagga cgatggcccc gtcgcgctgg cggtctttcc ccagcagata
     5101 atccaccggc ccggcaccgc cgcctcgccc tcgcggatga aacttaacga tcatcgtctg
     5161 cccccttttc cagcacggca tgccgcagcc gctcgagttc ggcatcaatg gccatcagcg
     5221 cggccaccac ctgaacccgg tcagcgcctg accactggcc gccgttaatt ttccgggcga
     5281 tctggttgag gttgttcccc atcccggcaa gctggcgcag caaaaccgga tcgatggacg
     5341 gcagccgcaa cgaccgcgcc gggcgcgtat cgagacaggt ttcccgcatc cacaccgcca
     5401 gttgccttcc attgcagcgc tccagcagct gctggtactc cccatcggtc acccggatac
     5461 ctaaaaaacg ggtacgctta accgccatcc tctcctccga tatccgcaaa atgtgaccgg
     5521 gctgaacgct caagctctgc catctgctcc cggcggtaat cttcctcctg tgcctgacgc
     5581 atcagatttg ccatca
//
