LOCUS       NZ_CP087643            11959 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02391 plasmid
            pDD02391-4, complete sequence.
ACCESSION   NZ_CP087643
VERSION     NZ_CP087643.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822723
            Assembly: GCF_023066725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11959)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11959)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066725.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:10:52
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,878
            CDSs (total)                      :: 5,756
            Genes (coding)                    :: 5,584
            CDSs (with protein)               :: 5,584
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 60 of 172
            Pseudo Genes (incomplete)         :: 113 of 172
            Pseudo Genes (internal stop)      :: 23 of 172
            Pseudo Genes (multiple problems)  :: 24 of 172
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087643.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11959
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02391"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02391-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            join(11952..11959,1..172)
                     /locus_tag="LMH57_RS29160"
                     /old_locus_tag="LMH57_29160"
     CDS             join(11952..11959,1..172)
                     /locus_tag="LMH57_RS29160"
                     /old_locus_tag="LMH57_29160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(199..729)
                     /locus_tag="LMH57_RS29165"
                     /old_locus_tag="LMH57_29165"
     CDS             complement(199..729)
                     /locus_tag="LMH57_RS29165"
                     /old_locus_tag="LMH57_29165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(736..1467)
                     /gene="mobC"
                     /locus_tag="LMH57_RS29170"
                     /old_locus_tag="LMH57_29170"
     CDS             complement(736..1467)
                     /gene="mobC"
                     /locus_tag="LMH57_RS29170"
                     /old_locus_tag="LMH57_29170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1467..3431)
                     /locus_tag="LMH57_RS29175"
                     /old_locus_tag="LMH57_29175"
     CDS             complement(1467..3431)
                     /locus_tag="LMH57_RS29175"
                     /old_locus_tag="LMH57_29175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            4563..4721
                     /locus_tag="LMH57_RS29430"
     CDS             4563..4721
                     /locus_tag="LMH57_RS29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            complement(4904..5053)
                     /locus_tag="LMH57_RS29180"
                     /old_locus_tag="LMH57_29180"
     CDS             complement(4904..5053)
                     /locus_tag="LMH57_RS29180"
                     /old_locus_tag="LMH57_29180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(5138..5395)
                     /locus_tag="LMH57_RS29185"
                     /old_locus_tag="LMH57_29185"
     CDS             complement(5138..5395)
                     /locus_tag="LMH57_RS29185"
                     /old_locus_tag="LMH57_29185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5405..7090)
                     /locus_tag="LMH57_RS29190"
                     /old_locus_tag="LMH57_29190"
     CDS             complement(5405..7090)
                     /locus_tag="LMH57_RS29190"
                     /old_locus_tag="LMH57_29190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(7195..8682)
                     /gene="ltrA"
                     /locus_tag="LMH57_RS29195"
                     /old_locus_tag="LMH57_29195"
     CDS             complement(7195..8682)
                     /gene="ltrA"
                     /locus_tag="LMH57_RS29195"
                     /old_locus_tag="LMH57_29195"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            9327..9572
                     /locus_tag="LMH57_RS29200"
                     /old_locus_tag="LMH57_29200"
     CDS             9327..9572
                     /locus_tag="LMH57_RS29200"
                     /old_locus_tag="LMH57_29200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            9846..10217
                     /gene="tolA"
                     /locus_tag="LMH57_RS29205"
                     /old_locus_tag="LMH57_29205"
     CDS             9846..10217
                     /gene="tolA"
                     /locus_tag="LMH57_RS29205"
                     /old_locus_tag="LMH57_29205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            10214..10579
                     /locus_tag="LMH57_RS29210"
                     /old_locus_tag="LMH57_29210"
     CDS             10214..10579
                     /locus_tag="LMH57_RS29210"
                     /old_locus_tag="LMH57_29210"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            10754..11032
                     /locus_tag="LMH57_RS29215"
                     /old_locus_tag="LMH57_29215"
     CDS             10754..11032
                     /locus_tag="LMH57_RS29215"
                     /old_locus_tag="LMH57_29215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            11020..11334
                     /locus_tag="LMH57_RS29220"
                     /old_locus_tag="LMH57_29220"
     CDS             11020..11334
                     /locus_tag="LMH57_RS29220"
                     /old_locus_tag="LMH57_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            11498..11926
                     /locus_tag="LMH57_RS29225"
                     /old_locus_tag="LMH57_29225"
     CDS             11498..11926
                     /locus_tag="LMH57_RS29225"
                     /old_locus_tag="LMH57_29225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     BGC             405..11959
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          4234..4801
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.795"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(740..1467)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 gcaggaaaaa actgccctca atatggctaa attcattaag gatcagtcgc tgctgttgct
       61 ggaaaagctg aacgagctgg acatggatac cccggccgac atgtgcgaac ggcttcacga
      121 ggatgcggaa acactctggc aggtgatgcg ggataagctc ggggaagaat aacagcaccg
      181 gccatgccgg tgctggcgtt attttttgcc gaacggccac caccggcggg cgggtttgtc
      241 ctccggcagt tcaggcaccg gcacgttaaa atagcggctg ttatattccc gcatcagctc
      301 cgctttcagc ttctcgttca ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat
      361 cgcgttaatc tgctccggtg agcacttccc ggcatcaacc acctgcacac cgttctcgat
      421 ggcgaaccgc tccacctttc gatagaggta atcacacagc ttcattcctg tgggtttgtc
      481 ccgttcccat tgcggatttt caccggtgaa aaagtacgtg gctttttcgt cgctgttgag
      541 ccgctcgtca tacatcccga tggatttcat catcgccaga tgctgggccc ggtcgacaaa
      601 acagaaaaca tacgccccgg tctgccaggc gagcaccacc atgtcttcca gatgctcgct
      661 ggttggcata atgcagcgct cgccgtcttt gtcttccacg tcctcaataa ataccatgta
      721 cagcgccata atccctcact ccgttgtcat aaatttaaag cacgcgaaca gcctctcccg
      781 gtccagctcc accgttttgc gggcctcgcc ggtctgtgga ttcacgattt catccaccgc
      841 cagaatcgcc cgctgtaccc ggacaagcgt cggccggtca gggcagatgt accagatttc
      901 atcgatgccg ttcgcccggc ggttgaacag atacccggac accacttcct gataccgcct
      961 gcggctcttg acggtgcgct ctacctcaaa tgccaccgtt ctgccgtcca gctccgccag
     1021 cgcgtccggc actttcatcc ctttcagggc catacggtgc aggtaccgcc agccggtggc
     1081 cccccggccc tccatggaga ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc
     1141 cgccgacacc cttgacggct gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa
     1201 cgagaacgcc accccgtgcg gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg
     1261 gtatttcgcc cggaccagca gccctttcgc caccagactg tccaggaagc ggtaaaccgg
     1321 ctgtaccgcc gcaatcccca gccgtcgcgc aaccagctcc gccgtggaac acagctcttc
     1381 ccgcagccag cgcagggtca gccgtgtttt ctccgcgttc cgcgcctgcc gcttcgcgtg
     1441 gctgacgttg tactgctcca gtgtcatcag taaagccccg caaaatcatc atccgctttt
     1501 ggtccgccgc cgtcggccgg aggcaggtac tccgccagcg ggtccgtttt cgccagcacc
     1561 gggaaggttt tcagcgaaat ttcccggcgc tcaacccgca gcgggctgct gaacgccagc
     1621 cgcgccacgc ccagcccggt catcaccgcg aacccgtcag gcaggtgttg cagggtgttc
     1681 acgtcaaaca gcggccgcgc ctccttctgg atgaagctgt cgccgctgat gtgctccacg
     1741 ttccccgcct cccggctggc ccggcggcgc tccacgtcca cccggatctc cccggtctgg
     1801 cccgccgccc actgcgcgga ctcctggctg gccgcgcgat agaaccagcg gatcggcgtg
     1861 ttatccagta ccgtggtttt cacaaaatca gcgggcagat cctgcccgca ctgaccgaag
     1921 tcgcccagcg actggtgcgc gagcagcagg ttgcagtccc ggctgcgcag cgtccccagc
     1981 gcgttcagca cgtacttcga cagcagatat ttaatctcat cgagcatgat gctcgcgtgc
     2041 ggccacctcc ggaactcatc ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc
     2101 ttctgcaccc ggatcaccgc ctcatcgtcc atcgagccga tgacgtacag gcagccgccg
     2161 ccgttgatta tccccgccac atcgataccg ctgtccgtct gcaggacgct caggtcggcc
     2221 acgttctcca gcttggtgat cagccccttc acgtcctttt tcagggcctc cggcaacgcg
     2281 tacgccgccg ccagcacctg ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc
     2341 ttcgccgctt tctgctcgct gatgcggtag tggtccgcca cgtcgccggt ctcactcagg
     2401 ttaaacccgg caaccagcag attcttcagg gcgtactggt cgagatcgcg gaacagattc
     2461 agctgcgccg gtttccccgg ccgcagatcc agcagggtga agtccgggca gtgcgcccgg
     2521 aagacgtgcg gcgcccacgc gtcccctttg gggtcgaagt acaccacagc gtccccgaaa
     2581 tcgcgtacgc agcgaatgag cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg
     2641 ccgaggatct gaatattggt cttgcgccag gtatcacgag ggaccagcac cggctggccc
     2701 tgctcatcct tacccagaaa caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg
     2761 gccagcgcgt taaccggtac cggctccccg aaatgcaggt ggcggacgtc tgtcatttct
     2821 ccggtccgct ggctgctgcg ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca
     2881 gcggccgcaa aatgaccatc aagcagcagg aacgccagca gccgcgccag aaggacgccg
     2941 gcgacaagcc ctccggcaca ccaggctatc ccgaacaggt tagccggctg cagcaggtaa
     3001 taatctgcgt tcgcatagag gtccgggcgc aggacggttt ctccccagct ctcgataaac
     3061 acggcgacaa ccagcccccc cagaagggcc agaccggcag cgccggtcag ccagtacgcg
     3121 tagttccaga catgtcgccg tgccctgacc atccgcccgg gtgaatccag cgaggccagc
     3181 accgaaaatg gcacagtccg gttattatcg cgcttatagc gatcggcata ctcccgccgc
     3241 gccttatggc tcagccccac aaaacgcatt cggccggccc agagttcagc cagaccgaaa
     3301 aacaggccga taaccagaaa aaaaccggcc agactgtaat actcccggta cagccagctg
     3361 acccacgggt aaatctgccg ggccagctcc gggatatgcc actgccaggc aagcgaatcc
     3421 gtattaaaca tgcaatctct ctccttcaga cgacgggttt accggcgtga tgcgcagccc
     3481 ccgctgcaca agccgcggac atgccccaag gcatggcggc ggcggtcacg tccgtggtga
     3541 ttgcgttcag ctttcaccgc ggaggccagc ctctttccgc cccatgcgga aaaggcgcag
     3601 ccccggagga acaacgagca cgccccagcg tgtgagagga aaaggcggca ccccagtgcg
     3661 gtcttttccg gtgttccggc ccccgtcaga gttcgccata gcgagcggct gaccacccgc
     3721 aaaatcagga gcagctttgc tgcttcgtca ggtgcgtcca gcaccgtgga tttttgtggg
     3781 tggtcaggcg tgagcctgac gggggccggg tgaatttctg gcgtggccgg cgtccagccg
     3841 gacagagcca gaaagagggg aaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcaa
     3901 ggtcttttcc cctgaagtca gccacgcccc agcggggatg acttgcattt ctccgcaagg
     3961 agaaaaaagc aacagagata ggttaacgta aaatgttcag cgaaaaaccg acatggttca
     4021 cctatcctga taattgatcg tcagggaata gaaagactta atcaggggac aatatcccac
     4081 atcagcgcgt ccggtgaccg tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc
     4141 ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc ggacacatac aaagttaccc
     4201 acagattccg tggataagca ggggactaac atgtgaggca aaacggcagg gccgcgccgg
     4261 tggcgttttt ccataggctc cgccctcctg ccagagttca cataaacaga cgctttttcg
     4321 gtgcatctgt gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac
     4381 aggacttaaa gatccccacc gtttccggta ggtcgctccc tcttgcgctc tcctgttccg
     4441 accctgccgt ttaccggata cctgttccgc ctttttccct ttcgggaagc gtggcgcttt
     4501 ctcatagctc acgctgctgg tatctcggct cggtttaggt cgttcgctcc aagctgggct
     4561 gtatgcacga accccccgtt cagcccgact gctgcgccct ttccggtaac tatcatcttg
     4621 agtccaaccc ggacggacac gcaaaaacgc cactggcagc agccattggt aactggatat
     4681 atacgagaga gatattgaga ggtcttgaag tgggtgccta actgcggcta cactgaaaga
     4741 acagttttgg tatctgcggt ctcgtatacc cagttacccg gttaagcagt tccccaactg
     4801 acttaacctt cgatcaaacc gcctccccag gcggtttttt cgtttacagt gcaagagatt
     4861 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc
     4921 ccgtcagttc agaggaggac gatggtgcca ccgttccgcc ctgaacatcc cggatataat
     4981 ttgcctgaca tgcggccaga agaaagccag caacaatcaa tataaaaaga aaaatagtct
     5041 ttaccttttt catatacttt ccctgtattt aaagactccg gcattcatcc ctgaacaccg
     5101 gaagtcagta tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat
     5161 caaaagctat ataacaatta tccacatcag gttttatttg atttttaaaa aatggctgta
     5221 gtaaaggaac ccattctttc ttcaaatcaa actcaccatt attaatatta tcctttaaag
     5281 gcattcccaa gctttcaatg acagaaccat cgtcaccaaa gtctttagaa tactcttccc
     5341 ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg taatttaagc cccatattta
     5401 cctctcaaag atatttttta atgttccgtt ttggatctgg ccctttaacc tgcttacccg
     5461 ttttgggatc aaatgcgcct aggtgttcgc catcactggc acgataccct tcaagctcac
     5521 cgtgctggga atcccattca taaattttac gttttttatc gccataccat cgggcccgct
     5581 taccaccacc accttgttta ggtgttttag gatcacctcg ctttaactct ccaagtccct
     5641 ttatgtcttc tgttttaggt actggatgat aatcatggcc gtagtcttta gtccccttgc
     5701 gaggcttgtt cttttcctca tttaacttat tttctgcatc cttcgctttt ttctcctttt
     5761 gtttccggct ctccatcgcc ccggtaagtg cagccttatc tctggataag gattcattca
     5821 ttgcgtttaa agatgcctga gccgctttta atttctcatt ctgattctga atatccttat
     5881 caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca
     5941 caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag
     6001 cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat
     6061 ttttatcttc ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt
     6121 ccggatgagc ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta
     6181 cctgtgccgg ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt
     6241 tcgggaaacg aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg
     6301 aatcaccttt ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg
     6361 gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt
     6421 ttgtcggttt cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat
     6481 gctgccgctc atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt
     6541 ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa
     6601 tttttgacat cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg
     6661 caatccccca cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg
     6721 cggccgacaa cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca
     6781 gggcaggcag accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc
     6841 tatgattgcc gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc
     6901 ccggattata gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc
     6961 tgtttggatc cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat
     7021 tcccactggc ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc
     7081 caccactcat accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatgtcg
     7141 cggtagggat acccgttacc ggataccccc cgcacagatc ccggcgtgcg cgatttacgc
     7201 accgggctcc tgcctcgggt gtctggcggt gaaccgctcc acaggccatg gatgaagaac
     7261 ccgaaccctt ggtagccatg cggctgccag tttgtttgct ttcgtccagg tcgtatcatc
     7321 cttctggctc ctgcgcctga gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt
     7381 ctgcatggtg gggaagttgc ccggtaccga gtgatagttc aggtatccct gaaccactct
     7441 cctgagccat tttccctgtt cggggattga gtaatgccag cgccttcgca gaccgtcttt
     7501 gatggctttc agagttgccg tcatccgatc ccggcgggtc tttcgtatca gcatgaacct
     7561 gccgttgcga tctttcccgc tgatgtgcgt gaacccgagg aagttgaacg tttctggttt
     7621 gccttttccc ctgatggcac ggttttcggc agcgaagcgg ccgaactcca tcagacgggt
     7681 tttctccggg tgaaccgtga gtccgaactc cctcagtctg cgctgcatgg ctatacggaa
     7741 gcgccgggca tcgtatcgtt tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat
     7801 taccacattg cctgtggcat agcgacgtcg ccactgatgc gcccacagat cgaagacgta
     7861 gtggaggtat atgtttgcca gcagcggtga gatgaccgca ccctgtgggg tgccttcctc
     7921 cgttgctcgc cattgaccct cctccgacgt cccggctgtg agccacttac gtatgagcct
     7981 gattaccctc cggtcgccga tccgatgctc tgtgaacctg atcagccatt cgtggctcac
     8041 cctgtcgaag aactgactga tgtcggcatc cagtacccag tttacgttag tgcgtaccag
     8101 ccctgtggcc agtgcgtcca gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga
     8161 gaaccccata aagtcgtttt catagactgc gttcaggatt ttcaccagcg catactggac
     8221 gatcttgtcc tccagcgagg cgatgccgag cgggcgttgt tttccatccg cttttgggat
     8281 gtagtgacgc ctgccgggct gcgccctgta gctgccctga tgtagcctcc ggtgcagatc
     8341 tgttatgttg ttcttcatgt ttccggcgta gtccatccac ctgatgccat ccactccggc
     8401 ggccgctttc ctgctcaggg agaggaatgc ggcttccagt gcttcgactg tcagcaggtg
     8461 gaacaatgct gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag
     8521 cctctgtgac atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc
     8581 cttggccccg ctccttcgct ccactgactc cgctcctttc gggttgttcg cctgcttcgc
     8641 cgctactatg agcgagtccg acttctcctc tccgtacatc accggctatg actcctcgtc
     8701 ttcccggtgc gggccatctc cgacactggc agatggtcag aggggagatc tcccggttcc
     8761 cgcgtagaga tcgtattgac atgccagggt ctcagacccc gccgggtcca tgtggcactc
     8821 gcagtatcgc accctatgat gttgccttcc gttaacagta caacgtcggc acccggtaat
     8881 ttaatataca tttcgtggct caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc
     8941 catacctcgc ggtatgcaac gcatgactcg gggaccttgt ggattgctgg tccttcaatg
     9001 gtcggggact ttcacccctt gatctctaac cggtctcccg gcgcacactg tacatataca
     9061 cagtataatt attaccttac tcctcccgtt ttcaggtgtc aatatatgag attagttctc
     9121 atcttcaggc acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa
     9181 aatttctcat ggcaacaaat tttattattt aagacattca tcacacttac atttcagatg
     9241 gtatagacac atctgaaagc taactggcat gaggagagga cctgcggtta aactgttcgg
     9301 gaaacctgag acgcccggag gccactatga cgcacctgaa actgactccc gctgaagaaa
     9361 agcgcctggc ggacgccttt gtcgctcagg cactggccat gggcctgaaa gggaacaaaa
     9421 cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc attcaggacg
     9481 aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa atacgaggct acggaaacca
     9541 ccttcacctg gcagccacag cgccgccggt aaggggtatt taccataacg tcgcattgcc
     9601 cgcaccgctc agacggtctg agggcgtttc cggcggctac gggataaaac aggcaacaaa
     9661 ccacccggaa aaccgccgga aacgcgcccg gtaaattgta acccgctgca tgcctatgca
     9721 cgcaactgca tggcgggccg tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa
     9781 tgaacgtggc cgtaaagagt gacaaatata catgtaacat tttttactga caggtcatca
     9841 acccgatgcg tttaataccc tttatcctgc tggcattgtg cagcccctta ctcctgacgg
     9901 cctgtaagca ttccaccacc cagccgccga aaatttatac tctggataag tcatctaaaa
     9961 tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt gaaccagagg
    10021 cctatgcggg aaaaatgtgc accgtgcgga tatccataca acgtgatggc actctgaaca
    10081 gcgccattgc tgaaggagga gatccgaaac tctgtaaagc agcaatatca gccgttaccc
    10141 acgccaaaat tccgccggca ccggatgaaa atacctggca aatattcaaa aacgcccctc
    10201 tggatttcag accatgaaag cttttactct gacagtgctg gcggccatgt tcctcactcc
    10261 ggtacatgcg ataccactaa aacccgtaac cacactgcag ccaacctacc cggtacaggc
    10321 cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt gatattctgg acgatggacg
    10381 agtggacaac gtgtcgctta ccggctgtca cctttttttt cgggaaacgc tgacggcaat
    10441 gaggcactgg ctatatcccg cagggcaccc ggcaatgcga caggttataa attttgaatt
    10501 cagtctgaaa aatgacggct tacaggatcc gcgaccagtc accacccaaa agctcaatga
    10561 tgatggttgc cgccagtaac aggtccttta acccgcaggc tgcaatagtc ctggatgatg
    10621 agtgagcctg tagcagccct gattagtgga agcgaagctg aaaaatgggc agcaaaagcc
    10681 gtatttctgg caaatagcgc cgccacggat taaagtagtc atgtgtaata catatgacta
    10741 caaaggggct tttatgtcat cagcaacatc catcaggctc gatgaagaac ttaaggaccg
    10801 gctgaaaacg ctggccgatg accggcaccg ctcagcgcac gcgctaatgc tggaggcaat
    10861 caccgaatac atcgaccggg aagagaagcg cagccagtac ctgcgggacg gacaggccgc
    10921 atggcagcac tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg aagcctggat
    10981 aagtacctgg ggaactgaga acgagcagga tgctccgcca tgccacaggt aacgatttcc
    11041 gcgctggcac agcgcgattt acaacgcctt caggactttc tcaaaaccaa aaatcggctg
    11101 gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa aacattgcca
    11161 gacattggcc gcccggtgcc gtttctgccg ctggaatata aggaactggt gatcgggttt
    11221 ggcgacagtg gctatgtgat gctctaccgc cacgaccggg aaatggacca gattgtgatc
    11281 gtcaccgtca ggcatcagaa agaatccggg tatccggggg cagacagcct ctaataaccg
    11341 atgacggggg cgcaattcat tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga
    11401 aaaccacatc ctgaacggaa aaacgggtaa aattcagcga tacatttacc gtgagaaatt
    11461 acacaccttt cacacccgat aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct
    11521 ttcacaggca gcacaagccg cgggcattac cagaagaacg ctatacaatc atgtaaaaca
    11581 gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact
    11641 gatccgtgta tacggaaatg tgaatatccc tgagaaacaa attcccggca tttcacaccg
    11701 tgaaaataca caaaaaaatt tcccacagga acagcttctg gcaatgcaaa aggaactggc
    11761 cgatctccgg caggctgtca ccctgatgct ggaagataaa acatcccggg aagaagaacg
    11821 ccgccagcac gatgatgagc gccggaaact ccaggccgaa gtggacagat tgacaacaga
    11881 actgacacag aagaaaaaac gcttctggtc cggctggttt agctgagaac ttaccgaagg
    11941 gaatggagaa catgacgaa
//
