LOCUS       NZ_CP087638             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02297 plasmid
            pDD02297-4, complete sequence.
ACCESSION   NZ_CP087638
VERSION     NZ_CP087638.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822724
            Assembly: GCF_023066705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066705.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 03:58:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,624
            CDSs (total)                      :: 5,502
            Genes (coding)                    :: 5,380
            CDSs (with protein)               :: 5,380
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 42 of 122
            Pseudo Genes (incomplete)         :: 80 of 122
            Pseudo Genes (internal stop)      :: 10 of 122
            Pseudo Genes (multiple problems)  :: 10 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087638.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02297"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02297-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            51..560
                     /locus_tag="LMH52_RS27980"
                     /old_locus_tag="LMH52_27980"
     CDS             51..560
                     /locus_tag="LMH52_RS27980"
                     /old_locus_tag="LMH52_27980"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            567..779
                     /locus_tag="LMH52_RS27985"
                     /old_locus_tag="LMH52_27985"
     CDS             567..779
                     /locus_tag="LMH52_RS27985"
                     /old_locus_tag="LMH52_27985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            1446..1670
                     /locus_tag="LMH52_RS27990"
                     /old_locus_tag="LMH52_27990"
     CDS             1446..1670
                     /locus_tag="LMH52_RS27990"
                     /old_locus_tag="LMH52_27990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            1654..1920
                     /locus_tag="LMH52_RS27995"
                     /old_locus_tag="LMH52_27995"
     CDS             1654..1920
                     /locus_tag="LMH52_RS27995"
                     /old_locus_tag="LMH52_27995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(1990..2340)
                     /locus_tag="LMH52_RS28000"
                     /old_locus_tag="LMH52_28000"
     CDS             complement(1990..2340)
                     /locus_tag="LMH52_RS28000"
                     /old_locus_tag="LMH52_28000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2406..2951)
                     /locus_tag="LMH52_RS28005"
                     /old_locus_tag="LMH52_28005"
     CDS             complement(2406..2951)
                     /locus_tag="LMH52_RS28005"
                     /old_locus_tag="LMH52_28005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(2977..3303)
                     /locus_tag="LMH52_RS28010"
                     /old_locus_tag="LMH52_28010"
     CDS             complement(2977..3303)
                     /locus_tag="LMH52_RS28010"
                     /old_locus_tag="LMH52_28010"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(3661..3819)
                     /locus_tag="LMH52_RS28160"
     CDS             complement(3661..3819)
                     /locus_tag="LMH52_RS28160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            4459..4635
                     /locus_tag="LMH52_RS28015"
                     /old_locus_tag="LMH52_28015"
     CDS             4459..4635
                     /locus_tag="LMH52_RS28015"
                     /old_locus_tag="LMH52_28015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            4625..4969
                     /locus_tag="LMH52_RS28020"
                     /old_locus_tag="LMH52_28020"
     CDS             4625..4969
                     /locus_tag="LMH52_RS28020"
                     /old_locus_tag="LMH52_28020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5151..5396)
                     /locus_tag="LMH52_RS28025"
                     /old_locus_tag="LMH52_28025"
     CDS             complement(5151..5396)
                     /locus_tag="LMH52_RS28025"
                     /old_locus_tag="LMH52_28025"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032433971.1"
                     /translation="MQPQSPVHHGLPRIDARTVCPVVVRLETPAGQQFRVRYQKIQFQP
                     PLVPVFNPQHAVLVFIESGHQNPLEARHQLFTLAGR"
                     /name="hypothetical protein"
     Typing          4294..4373
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(3711..4108)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          3..895
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.664"
                     /coverage="99.0"
                     /evalue="1.8899999999999998e-172"
     Typing          4960..5594
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="3.66e-135"
ORIGIN
        1 cattgccgac ccggacggag ggcgaaacat ccgacttaag ggagccatct atgaacagtc
       61 ttttaacgct ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag agtaccggcg
      121 aaatgctgaa agccgcattc agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa
      181 gcgggaagag aatcagcgcc gccatccgcg cccacgagca gggtatgagc gcggccatgc
      241 agtcgaaccg cctgagcgtg atgcgtatgg tcgggcggac atggctgacc atcacgatgg
      301 tgtcaggact gctgttcgcc agcctgagcg gggtgttgtg gtatcagggg agcttgatag
      361 cgtcaaatct ggccgaaatc gaccggcaga acgcagcgct gtcgaagctg aacgcgaaga
      421 cctggggcgt gacgtacctg gaagacagca acggacgttt tctggtgctg ccgaagggga
      481 cggccgtaga caggacgcag agctggacgg tcgggaacgg gcggtcgaag cagaacgcgc
      541 tgagactggt gaaggagtaa gtgccgatga ccgagctgga aaaacagttg ctgagcgcat
      601 tagagcagct acagcaggac tactcgaaaa ggctggacga gtgggagagc gccttcgggg
      661 aatggcggaa aatgtcaggg cttatgcaac gggagaacgc ggcgctgagc gagcgcgtga
      721 cgcgcttgag tcagcaggtg gaacgcttga gcgggcaact gcagcgtttg agccggtagt
      781 gcagcgtcat gagatggccg tggccgcaga acgggcgcac gagcaacggc agcacgaaaa
      841 ggaactgacg gaagcgcgtt cccggcagcg aagctatgac ggtccgtcgc tgggctgaaa
      901 tgcaggaaaa cagggttctc atcgggcatt tacgctgaaa ccggatgtca ttcacctggc
      961 tgcatggcta tgcagccagg taaaatttat ctgtcgcgaa cggggcggtt tatcgggtgg
     1021 tttgttgccg gttttacctg actgccaccc cgttcgcggc gaacccgtcc ggggcggtgc
     1081 gggcaacggg tgttatgttt aatcagcgtg aacggcagat acaaaaaagc cccggagggc
     1141 ttttaactac tgacttattt ttttgaagat ttgctgggtt tcaatttgtc agtatttccc
     1201 ggcgtttttt tattgtcacg ctggcgttta tcaggttccc ctgttgaatc ggcaattcgt
     1261 ttcggacccg gcttaagacc cataatcgtt atcctttcaa tagttaagag gaaagccctc
     1321 aagctagata tattgaccgc gttacggatt atcaagaggg gctaacaaga ttattttttg
     1381 ttcttttagg ttgattgtgg tactgttgat atacatgtat agcaaatggt gttcgggagg
     1441 tttatatgct tgctatccgg ttatctgatg agattgagtc ccgtctggac tcgctggcga
     1501 agcaaaccgg cagaacaaag acgttttatg cgcgggaagc aatactggcg catctggaag
     1561 acctggagga ttattatctt tcagcagaaa ctgctgcacg cgttcgccgt ggtgatgaag
     1621 cagtgcattc gtctgaagac gtgaggaagt cacttggtct ggacgattaa ctattccgat
     1681 cgggcgctca aatcgttacg caagatggac aaacagaacg cacgacggat tgtggatttt
     1741 atgagtttac gcattgcagt tgctgccgat cctcgccagt cagggaagcc gctcaaaggt
     1801 gagctgggcg agttctggcg ctatcgggtg ggagattatc gcgttctgtg tgagatccga
     1861 gatgacgagc ttgttatcct tgccgccacg attggacatc gccgcgaagt ttacgactga
     1921 agcccgctat gcgggctttt ttgatgccga caacaggtat tatggtaaat actcgactaa
     1981 ctacgtgttc tactgtttgt cggttttagt taaccagcag gttgtcttaa cgataatgta
     2041 gccgttattt ttaactaata aggtcatatt gttaacgtca ggcataataa aagacattat
     2101 ggcctcagag ttatcatcag aagcagctct ctgcattact acttcgattc ccttttcttt
     2161 gaatctataa gtatccagtt gcctattaaa cttagaaaga cctacaacca tacttagttt
     2221 gttatcttta acagtaagag atgcttcctg agtaccatta catcggtagt aagcggtgta
     2281 accgtcagca aaagatcgac cggggaatat aaatgctagg aacgcaatta accaatacat
     2341 agttgagaat cttggtatta cacatttcac tatcctctct ttcatttttc gttcaccata
     2401 ctttgttatc tgatttttaa tttttctatt ttataaacca agtctctacc aaaaaggcga
     2461 aaattatcct caaataataa taattgttta agaacatcca tgaaagcagc tgcttcaccc
     2521 tcagttatgg gagctactct atataacata taacctgagt taagggtttc ttttatactt
     2581 tcttcaatgc tcattataaa tattagtgga ttatcatttt tcttccccca tgggtttcta
     2641 attaatcctt cgatgtactc aggtcctttg cgtcctattg cctgcgcaat attttttatt
     2701 tcatctgcgt gagggctttg ttgtacagct ttgtttatgc ctgcttgagt gattttcaga
     2761 gactctacac cgtgtatgtt gtatgagata aaatgtaata aattaagatg caatgcaatt
     2821 aatccatttt tgtttacatc tgagtatttg gataaaaaac gtaatgaagt gttaactctc
     2881 ccttgaagcc taacaattgg gagtgctgcc ttgtatattt ccttctccgt taaataccgt
     2941 ttaaaaaaca taattctccg cctccgtata gcgtgcctat tttatttcgt cgtaaaactt
     3001 agctctgaaa tattccttta gaaccatcat gtcattaata attgaatcta tctcatccat
     3061 tttttcttgg tattctttta atggtgaagg gagcggtaac tcaatatttg aaaagtgatt
     3121 ggcgagatag ggatcaaggt ttctcatcca ttcaggcttt tcgccaatgc gttcttcaac
     3181 tgaaagtgaa cctgataatc tctggttaat aagatcactt agactcattt tggttttatt
     3241 cgaaagccgt tgtaaggatt gctttaatcc tatatctagc atgatatttg ttctttctct
     3301 cattaccccc ccttgctatt gcgttttttg ttgcgcacaa agctaccatt gcgcattaag
     3361 aaacgcaata aaaaaggagg gagggagtgg tttgtacatg atgtgcagtg cgctgagggc
     3421 atcagtggcg ggtgtttttc cgtgggatgg atggagctcc ggggataaca gaactgacga
     3481 tcatttaaag gatcttcttg agatcctttt tttctgcgcg taatctcttg cactgtaaac
     3541 gaaaaaaccg cctggggagg cggtttgatc gaaggttaag tcagttgggg aactgcttaa
     3601 ccgggtaact gggtatacga gaccgcagat accaaaactg ttctttcagt gtagccgcag
     3661 ttaggcaccc acttcaagac ctctcaatat ctctctcgta tatatccagt taccaatggc
     3721 tgctgccagt ggcgtttttg cgtgtccgtc cgggttggac tcaagatgat agttaccgga
     3781 aagggcgcag cagtcgggct gaacgggggg ttcgtgcata cagcccagct tggagcgaac
     3841 gacctaaacc gagccgagat accagcagcg tgagctatga gaaagcgcca cgcttcccga
     3901 aagggaaaaa ggcggaacag gtatccggta aacggcaggg tcggaacagg agagcgcacg
     3961 agggagccgc cggagagaaa tcaccggcat ctttaagtcc tgtcgggttt cgtcctcctc
     4021 tgacttgagc gtccatttct gtgatgctcg tcaggggagg cggagcctat ggaaaaacgg
     4081 caacgccgca tccttttctc cggccctttc tgatttgagc gcctttgctt tatcccgtta
     4141 tccgtgagtc ggtcacgccc gccgcagccg aacggcagga gcgcagctac cgagtgagcg
     4201 aggaggcgtc atttcatctg tccgtgcatg gtattgcgga cgctcctgag ccacatatca
     4261 cacctgccac atgaggcact ttcatgaatc gtgctctcat gccaacatag taagccagta
     4321 tacacagcgt aaacgctgtg actggttcag ggctgcgccc cgaaacccgc taacaccact
     4381 gacgcgctgg cgcttgtcca acgccggagc gacccggaaa acttttccgt gccgtccggc
     4441 gtttgcgaag gagggataat gcagaagccg aagaagaagc cgctcgtcgt aaaaaaaaga
     4501 tcctggcatc cggcagtgat ggcaaatctg atgcgtcagg cacaggagga agattaccgc
     4561 cgggagcaga tggcagagct tgagcgttca gcccggtcac attttgcgga tatcggagga
     4621 gaggatggcg gttaagcgta cccgtttttt aggtatccgg gtgaccgatg gggagtacca
     4681 gcagctgctg gagcgctgca atggaaggca actggcggtg tggatgcggg aaacctgtct
     4741 cgatacgcgc ccggcgcggt cgttgcggct gccgtccatc gatccggttt tgctgcgcca
     4801 gcttgccggg atggggaaca acctcaacca gatcgcccgg aaaattaacg gcggccagtg
     4861 gtcaggcgct gaccgggttc aggtggtggc cgcgctgatg gccattgatg ccgaactcga
     4921 gcggctgcgg catgccgtgc tggaaaaggg ggcagacgat gatcgttaag tttcatccgc
     4981 gagggcgagg cggcggtgcc gggccggtgg attatctgct ggggaaagac cgccagcgcg
     5041 acggggccat cgtcctgcag ggaaagccgg aggaggtccg ggagcttatc gacgcctcgc
     5101 cctacgccaa aaagtacacc tccggcgtcc tgtcttttgc tgaacaggat ttaccgcccg
     5161 gccagcgtga aaagctgatg gcgagcttcg agcgggttct gatgcccgga ctcgataaag
     5221 accagtacag cgtgctgtgg gttgaacacc gggacaaggg gcggctggaa ctgaattttc
     5281 tggtaccgaa cacggaactg ctgaccggca ggcgtctcca gccgtactac gaccgggcag
     5341 accgtccgcg catcgatgcg tggcagaccg tggtgaacgg gcgactgggg ctgcacgacc
     5401 cgaacgcacc ggagaaccgg cgcgcgctgg tgacgccatc ttcgttaccc aaaacgaagc
     5461 aggaagccgc agaggctatt acgcggggct tactggccct cgcctcgtca ggggagctta
     5521 aaacgcgtca ggacgtcact gaggcgctgg aaagcgcagg ttttgaggtc gtgcgcacca
     5581 caaagagcag catcag
//
