LOCUS       NZ_CP087637            10062 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02297 plasmid
            pDD02297-3, complete sequence.
ACCESSION   NZ_CP087637
VERSION     NZ_CP087637.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822724
            Assembly: GCF_023066705.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066705.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 03:58:22
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,624
            CDSs (total)                      :: 5,502
            Genes (coding)                    :: 5,380
            CDSs (with protein)               :: 5,380
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 122
            CDSs (without protein)            :: 122
            Pseudo Genes (ambiguous residues) :: 0 of 122
            Pseudo Genes (frameshifted)       :: 42 of 122
            Pseudo Genes (incomplete)         :: 80 of 122
            Pseudo Genes (internal stop)      :: 10 of 122
            Pseudo Genes (multiple problems)  :: 10 of 122
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087637.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02297"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02297-3"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            join(9919..10062,1..228)
                     /gene="tolA"
                     /locus_tag="LMH52_RS27915"
                     /old_locus_tag="LMH52_27915"
     CDS             join(9919..10062,1..228)
                     /gene="tolA"
                     /locus_tag="LMH52_RS27915"
                     /old_locus_tag="LMH52_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            225..590
                     /locus_tag="LMH52_RS27920"
                     /old_locus_tag="LMH52_27920"
     CDS             225..590
                     /locus_tag="LMH52_RS27920"
                     /old_locus_tag="LMH52_27920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            765..1043
                     /locus_tag="LMH52_RS27925"
                     /old_locus_tag="LMH52_27925"
     CDS             765..1043
                     /locus_tag="LMH52_RS27925"
                     /old_locus_tag="LMH52_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1031..1345
                     /locus_tag="LMH52_RS27930"
                     /old_locus_tag="LMH52_27930"
     CDS             1031..1345
                     /locus_tag="LMH52_RS27930"
                     /old_locus_tag="LMH52_27930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1509..1937
                     /locus_tag="LMH52_RS27935"
                     /old_locus_tag="LMH52_27935"
     CDS             1509..1937
                     /locus_tag="LMH52_RS27935"
                     /old_locus_tag="LMH52_27935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1963..2142
                     /locus_tag="LMH52_RS27940"
                     /old_locus_tag="LMH52_27940"
     CDS             1963..2142
                     /locus_tag="LMH52_RS27940"
                     /old_locus_tag="LMH52_27940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2169..2699)
                     /locus_tag="LMH52_RS27945"
                     /old_locus_tag="LMH52_27945"
     CDS             complement(2169..2699)
                     /locus_tag="LMH52_RS27945"
                     /old_locus_tag="LMH52_27945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2706..3437)
                     /gene="mobC"
                     /locus_tag="LMH52_RS27950"
                     /old_locus_tag="LMH52_27950"
     CDS             complement(2706..3437)
                     /gene="mobC"
                     /locus_tag="LMH52_RS27950"
                     /old_locus_tag="LMH52_27950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3437..5401)
                     /locus_tag="LMH52_RS27955"
                     /old_locus_tag="LMH52_27955"
     CDS             complement(3437..5401)
                     /locus_tag="LMH52_RS27955"
                     /old_locus_tag="LMH52_27955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6887..7036)
                     /locus_tag="LMH52_RS27960"
                     /old_locus_tag="LMH52_27960"
     CDS             complement(6887..7036)
                     /locus_tag="LMH52_RS27960"
                     /old_locus_tag="LMH52_27960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7121..7378)
                     /locus_tag="LMH52_RS27965"
                     /old_locus_tag="LMH52_27965"
     CDS             complement(7121..7378)
                     /locus_tag="LMH52_RS27965"
                     /old_locus_tag="LMH52_27965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7388..9073)
                     /locus_tag="LMH52_RS27970"
                     /old_locus_tag="LMH52_27970"
     CDS             complement(7388..9073)
                     /locus_tag="LMH52_RS27970"
                     /old_locus_tag="LMH52_27970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9400..9645
                     /locus_tag="LMH52_RS27975"
                     /old_locus_tag="LMH52_27975"
     CDS             9400..9645
                     /locus_tag="LMH52_RS27975"
                     /old_locus_tag="LMH52_27975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             2388..10062
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6204..6784
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.603"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2710..3437)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gctgttgaaa tgcagatgtt tgaaccagag gcctatgcgg gaaaaatgtg caccgtgcgg
       61 atatccatac aacgtgatgg cactctgaac agcgccattg ctgaaggagg agatccgaaa
      121 ctctgtaaag cagcaatatc agccgttacc cacgccaaaa ttccgccggc accggatgaa
      181 aatacctggc aaatattcaa aaacgcccct ctggatttca gaccatgaaa gcttttactc
      241 tgacagtgct ggcggccatg ttcctcactc cggtacatgc gataccacta aaacccgtaa
      301 ccacactgca gccaacctac ccggtacagg ccgcagcaaa acgcatcgaa ggtcaggtga
      361 ccgccacgtt tgatattctg gacgatggac gagtggacaa cgtgtcgctt accggctgtc
      421 accttttttt tcgggaaacg ctgacggcaa tgaggcactg gctatatccc gcagggcacc
      481 cggcaatgcg acaggttata aattttgaat tcagtctgaa aaatgacggc ttacaggatc
      541 cgcgaccagt caccacccaa aagctcaatg atgatggttg ccgccagtaa caggtccttt
      601 aacccgcagg ctgcaatagt cctggatgat gagtgagcct gtagcagccc tgattagtgg
      661 aagcgaagct gaaaaatggg cagcaaaagc cgtatttctg gcaaatagcg ccgccacgga
      721 ttaaagtagt catgtgtaat acatatgact acaaaggggc ttttatgtca tcagcaacat
      781 ccatcaggct cgatgaagaa cttaaggacc ggctgaaaac gctggccgat gaccggcacc
      841 gctcagcgca cgcgctaatg ctggaggcaa tcaccgaata catcgaccgg gaagagaagc
      901 gcagccagta cctgcgggac ggacaggccg catggcagca ctatcaggaa acgggtctgc
      961 accttactgc ggaagaagcg gaagcctgga taagtacctg gggaactgag aacgagcagg
     1021 atgctccgcc atgccacagg taacgatttc cgcgctggca cagcgcgatt tacaacgcct
     1081 tcaggacttt ctcaaaacca aaaatcggct ggcagccaga aaaggaggtg aggtgatcgt
     1141 ccgggctatc cagcaactga aaacattgcc agacattggc cgcccggtgc cgtttctgcc
     1201 gctggaatat aaggaactgg tgatcgggtt tggcgacagt ggctatgtga tgctctaccg
     1261 ccacgaccgg gaaatggacc agattgtgat cgtcaccgtc aggcatcaga aagaatccgg
     1321 gtatccgggg gcagacagcc tctaataacc gatgacgggg gcgcaattca ttgcgtggcc
     1381 gtcgccggtt tgacaagcgc agcgcgtcag aaaaccacat cctgaacgga aaaacgggta
     1441 aaattcagcg atacatttac cgtgagaaat tacacacctt tcacacccga taaaatcagg
     1501 ggtatacagt ggcgaaaaca ttgctcaacc tttcacaggc agcacaagcc gcgggcatta
     1561 ccagaagaac gctatacaat catgtaaaac agggaaaggt taccgtgtca cgtgacggga
     1621 aaaacaaccc tgtcgtggat gtgtcagaac tgatccgtgt atacggaaat gtgaatatcc
     1681 ctgagaaaca aattcccggc atttcacacc gtgaaaatac acaaaaaaat ttcccacagg
     1741 aacagcttct ggcaatgcaa aaggaactgg ccgatctccg gcaggctgtc accctgatgc
     1801 tggaagataa aacatcccgg gaagaagaac gccgccagca cgatgatgag cgccggaaac
     1861 tccaggccga agtggacaga ttgacaacag aactgacaca gaagaaaaaa cgcttctggt
     1921 ccggctggtt tagctgagaa cttaccgaag ggaatggaga acatgacgaa gcaggaaaaa
     1981 actgccctca atatggctaa attcattaag gatcagtcgc tgctgttgct ggaaaagctg
     2041 aacgagctgg acatggatac cccggccgac atgtgcgaac ggcttcacga ggatgcggaa
     2101 acactctggc aggtgatgcg ggataagctc ggggaagaat aacagcaccg gccatgccgg
     2161 tgctggcgtt attttttgcc gaacggccac caccggcggg cgggtttgtc ctccggcagt
     2221 tcaggcaccg gcacgttaaa atagcggctg ttatattccc gcatcagctc cgctttcagc
     2281 ttctcgttca ccgcgctttc cccgtgccgc tcgctgacca cctcaatcat cgcgttaatc
     2341 tgctccggtg agcacttccc ggcatcaacc acctgcacac cgttctcgat ggcgaaccgc
     2401 tccacctttc gatagaggta atcacacagc ttcattcctg tgggtttgtc ccgttcccat
     2461 tgcggatttt caccggtgaa aaagtacgtg gctttttcgt cgctgttgag ccgctcgtca
     2521 tacatcccga tggatttcat catcgccaga tgctgggccc ggtcgacaaa acagaaaaca
     2581 tacgccccgg tctgccaggc gagcaccacc atgtcttcca gatgctcgct ggttggcata
     2641 atgcagcgct cgccgtcttt gtcttccacg tcctcaataa ataccatgta cagcgccata
     2701 atccctcact ccgttgtcat aaatttaaag cacgcgaaca gcctctcccg gtccagctcc
     2761 accgttttgc gggcctcgcc ggtctgtgga ttcacgattt catccaccgc cagaatcgcc
     2821 cgctgtaccc ggacaagcgt cggccggtca gggcagatgt accagatttc atcgatgccg
     2881 ttcgcccggc ggttgaacag atacccggac accacttcct gataccgcct gcggctcttg
     2941 acggtgcgct ctacctcaaa tgccaccgtt ctgccgtcca gctccgccag cgcgtccggc
     3001 actttcatcc ctttcagggc catacggtgc aggtaccgcc agccggtggc cccccggccc
     3061 tccatggaga ggcgcaggga ctgcacggcc agccggtgcg gcagctgcgc cgccgacacc
     3121 cttgacggct gaaacggaat aacgtccgtc agcggctcgt cctcgtcgaa cgagaacgcc
     3181 accccgtgcg gcgtcagccc ccacacgctg acctgacggc cgtcaaccgg gtatttcgcc
     3241 cggaccagca gccctttcgc caccagactg tccaggaagc ggtaaaccgg ctgtaccgcc
     3301 gcaatcccca gccgtcgcgc aaccagctcc gccgtggaac acagctcttc ccgcagccag
     3361 cgcagggtca gccgtgtttt ctccgcgttc cgcgcctgcc gcttcgcgtg gctgacgttg
     3421 tactgctcca gtgtcatcag taaagccccg caaaatcatc atccgctttt ggtccgccgc
     3481 cgtcggccgg aggcaggtac tccgccagcg ggtccgtttt cgccagcacc gggaaggttt
     3541 tcagcgaaat ttcccggcgc tcaacccgca gcgggctgct gaacgccagc cgcgccacgc
     3601 ccagcccggt catcaccgcg aacccgtcag gcaggtgttg cagggtgttc acgtcaaaca
     3661 gcggccgcgc ctccttctgg atgaagctgt cgccgctgat gtgctccacg ttccccgcct
     3721 cccggctggc ccggcggcgc tccacgtcca cccggatctc cccggtctgg cccgccgccc
     3781 actgcgcgga ctcctggctg gccgcgcgat agaaccagcg gatcggcgtg ttatccagta
     3841 ccgtggtttt cacaaaatca gcgggcagat cctgcccgca ctgaccgaag tcgcccagcg
     3901 actggtgcgc gagcagcagg ttgcagtccc ggctgcgcag cgtccccagc gcgttcagca
     3961 cgtacttcga cagcagatat ttaatctcat cgagcatgat gctcgcgtgc ggccacctcc
     4021 ggaactcatc ccgcgcaatg atgatttgcg cgcagcgggc aaacagcatc ttctgcaccc
     4081 ggatcaccgc ctcatcgtcc atcgagccga tgacgtacag gcagccgccg ccgttgatta
     4141 tccccgccac atcgataccg ctgtccgtct gcaggacgct caggtcggcc acgttctcca
     4201 gcttggtgat cagccccttc acgtcctttt tcagggcctc cggcaacgcg tacgccgccg
     4261 ccagcacctg ctgaatgttt gccccctgcg ggaactgctc cgcaatcagc ttcgccgctt
     4321 tctgctcgct gatgcggtag tggtccgcca cgtcgccggt ctcactcagg ttaaacccgg
     4381 caaccagcag attcttcagg gcgtactggt cgagatcgcg gaacagattc agctgcgccg
     4441 gtttccccgg ccgcagatcc agcagggtga agtccgggca gtgcgcccgg aagacgtgcg
     4501 gcgcccacgc gtcccctttg gggtcgaagt acaccacagc gtccccgaaa tcgcgtacgc
     4561 agcgaatgag cgcgttggtg gccatcgcgc tcttgccgct cccgggcagg ccgaggatct
     4621 gaatattggt cttgcgccag gtatcacgag ggaccagcac cggctggccc tgctcatcct
     4681 tacccagaaa caccgcctgc tgtttccggg cctgctccgt ggaaaaatcg gccagcgcgt
     4741 taaccggtac cggctccccg aaatgcaggt ggcggacgtc tgtcatttct ccggtccgct
     4801 ggctgctgcg ccggctctcc tgattcagtt tcgcgttcac cgcctcgtca gcggccgcaa
     4861 aatgaccatc aagcagcagg aacgccagca gccgcgccag aaggacgccg gcgacaagcc
     4921 ctccggcaca ccaggctatc ccgaacaggt tagccggctg cagcaggtaa taatctgcgt
     4981 tcgcatagag gtccgggcgc aggacggttt ctccccagct ctcgataaac acggcgacaa
     5041 ccagcccccc cagaagggcc agaccggcag cgccggtcag ccagtacgcg tagttccaga
     5101 catgtcgccg tgccctgacc atccgcccgg gtgaatccag cgaggccagc accgaaaatg
     5161 gcacagtccg gttattatcg cgcttatagc gatcggcata ctcccgccgc gccttatggc
     5221 tcagccccac aaaacgcatt cggccggccc agagttcagc cagaccgaaa aacaggccga
     5281 taaccagaaa aaaaccggcc agactgtaat actcccggta cagccagctg acccacgggt
     5341 aaatctgccg ggccagctcc gggatatgcc actgccaggc aagcgaatcc gtattaaaca
     5401 tgcaatctct ctccttcaga cgacgggttt accggcgtga tgcgcagccc ccgctgcaca
     5461 agccgcggac atgccccaag gcatggcggc ggcggtcacg tccgtggtga ttgcgttcag
     5521 ctttcaccgc ggaggccagc ctctttccgc cccatgcgga aaaggcgcag ccccggagga
     5581 acaacgagca cgccccagcg tgtgagagga aaaggcggca ccccagtgcg gtcttttccg
     5641 gtgttccggc ccccgtcaga gttcgccata gcgagcggct gaccacccgc aaaatcagga
     5701 gcagctttgc tgcttcgtca ggtgcgtcca gcaccgtgga tttttgtggg tggtcaggcg
     5761 tgagcctgac gggggccggg tgaatttctg gcgtggccgg cgtccagccg gacagagcca
     5821 gaaagagggg aaaagaccgt gcgtcggtgg cgtccagcca gcgtaagcaa ggtcttttcc
     5881 cctgaagtca gccacgcccc agcggggatg acttgcattt ctccgcaagg agaaaaaagc
     5941 aacagagata ggttaacgta aaatgttcag cgaaaaaccg acatggttca cctatcctga
     6001 taattgatcg tcagggaata gaaagactta atcaggggac aatatcccac atcagcgcgt
     6061 ccggtgaccg tcttgcaggt gccggggaaa ttgcgcctcc tcgctcactc ggtcgctacg
     6121 ctccgggcgt gagactgcgg cgggcgctgc ggacacatac aaagttaccc acagattccg
     6181 tggataagca ggggactaac atgtgaggca aaacggcagg gccgcgccgg tggcgttttt
     6241 ccataggctc cgccctcctg ccagagttca cataaacaga cgctttttcg gtgcatctgt
     6301 gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac aggacttaaa
     6361 gatccccacc gtttccggta ggtcgctccc tcttgcgctc tcctgttccg accctgccgt
     6421 ttaccggata cctgttccgc cgttctccct tgcgggaagt gtggcgcttt ctcatagctc
     6481 acactactgg tatctcggct cggtgtaggt cgttcgctcc aagctgggct gtaagcaaga
     6541 actccccgtt cagcccgact gctgcgcctt atccggtaac tttcatcttg agtccaaccc
     6601 ggaaagacac gcaaaaacgc cactggcagc agccattggt aactgggagt tcgcagagga
     6661 tttgtttagc taaacacgcg gttgctcttg aagtgtgcgc caaagtccgg ctacactgga
     6721 aggacagatt tggttgctgt gctctgcgaa agccagttac ccggttaagc agttccccaa
     6781 ctgacttaac cttcgatcaa accacctccc caggtggttt tttcgtttac agggcaaaag
     6841 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac tgaaccgcga
     6901 tccccgtcag ttcagaggag gacgatggtg ccaccgttcc gccctgaaca tcccggatat
     6961 aatttgcctg acatgcggcc agaagaaagc cagcaacaat caatataaaa agaaaaatag
     7021 tctttacctt tttcatatac tttccctgta tttaaagact ccggcattca tccctgaaca
     7081 ccggaagtca gtatttcaaa tcatccttga taattttttg ttaccagtta ccgtcccgat
     7141 aatcaaaagc tatataacaa ttatccacat caggttttat ttgattttta aaaaatggct
     7201 gtagtaaagg aacccattct ttcttcaaat caaactcacc attattaata ttatccttta
     7261 aaggcattcc caagctttca atgacagaac catcgtcacc aaagtcttta gaatactctt
     7321 cccctttaaa ctcttcagtt ttcttatcaa accatgtcaa acgtaattta agccccatat
     7381 ttacctctca aagatatttt ttaatgttcc gttttggatc tggcccttta acctgcttac
     7441 ccgttttggg atcaaatgcg cctaggtgtt cgccatcact ggcacgatac ccttcaagct
     7501 caccgtgctg ggaatcccat tcataaattt tacgtttttt atcgccatac catcgggccc
     7561 gcttaccacc accaccttgt ttaggtgttt taggatcacc tcgctttaac tctccaagtc
     7621 cctttatgtc ttctgtttta ggtactggat gataatcatg gccgtagtct ttagtcccct
     7681 tgcgaggctt gttcttttcc tcatttaact tattttctgc atccttcgct tttttctcct
     7741 tttgtttccg gctctccatc gccccggtaa gtgcagcctt atctctggat aaggattcat
     7801 tcattgcgtt taaagatgcc tgagccgctt ttaatttctc attctgattc tgaatatcct
     7861 tatcaagata gcgaacctga tcctgccacc cggagccata ttcatgaggc gggttataag
     7921 tcacaaaatc atctttattt gcctctgcct cttttacctt tttatcggct tcctgaacag
     7981 cagcccttgc accggggagc gccttctctg tcgatgcaat gcggctgttt aaggtcgcaa
     8041 tatttttatc ttcggcatcc agctcagctt tcgctttatc atagtctctt ttcagcccct
     8101 cttccggatg agcggcgtcc catgcctgct ggcgacgctt ttcttcctcc tgacgctgtt
     8161 ttacctgtgc cggggtaaga acatctgtca ccgacacata aaccggcttc tgtccggtct
     8221 ctttcgggaa acgaataacg gcctcacggg agttaccacc ggctgtaaaa ccagccggac
     8281 gtgaatcacc tttttcagca acaatgcctt ttggcggggc tttcgctgtc ggaacacctt
     8341 tcggtacgct cacctgcaga gacgggagac ccggaatcga cacactgaat acccccggac
     8401 gttttgtcgg tttcgcatcc accacaggga cactcattgg ccgacctgcg acaaccgcaa
     8461 tatgctgccg ctcatccttc accacatcca caacccgttg tctgacacga accgtcgcct
     8521 ggtccagtgg taaagtactt gccggagtct ccgtcaccgc atcggccggt aatgacgtca
     8581 taatttttga catcattttc ggatcatctt ttgcaatctc agaaggcagc acaccgtaga
     8641 tcgcaatccc ccacagacca aacttaaacg gccccttcag ggcagccagc acatcagcaa
     8701 cggcggccga caacgcatcg ccggaaactg ataaagccag tccttcagcc cccggagtag
     8761 ccagggcagg cagaccgaag gccatcgcag tggccgaaga ctgtccgcca ccagagctac
     8821 cgctatgatt gccgctgtta ccaccattac caccgggttt actcccgccg ttaccaaact
     8881 cccccggatt atagttatga atatctccat tcggcgtatg cgtcgtaccc cagcccggcc
     8941 cgctgtttgg atccgtacca ccacctgatg aagggccacc tttaccagac gtaccgttca
     9001 cattcccact ggcctgacca ccattatgtc ccagacctga attacccgga cctcgaccgt
     9061 ctccaccact cataccgttt tcctctttga catttaaaac aataaattaa agccaacata
     9121 ctgtacatat acacagtata attattacct tactcctccc gttttcaggt gtcaatatat
     9181 gagattagtt ctcatcttca ggcacaaaaa agcgcccgga gccacctgta agcagatcgc
     9241 gtcttttccc gaaaatttct catggcaaca aattttatta tttaagacat tcatcacact
     9301 tacatttcag atggtataga cacatctgaa agctaactgg catgaggaga ggacctgcgg
     9361 ttaaactgtt cgggaaacct gagacgcccg gaggccacta tgacgcacct gaaactgact
     9421 cccgctgaag aaaagcgcct ggcggacgcc tttgtcgctc aggcactggc catgggcctg
     9481 aaagggaaca aaacgctgcc aaaggcggta aaacaacagg tgcgggaaag ggtgcttcgt
     9541 gccattcagg acgaacgtga agccaggcgg gcgcaggaaa tgcgtcggga gaaatacgag
     9601 gctacggaaa ccaccttcac ctggcagcca cagcgccgcc ggtaaggggt atttaccata
     9661 acgtcgcatt gcccgcaccg ctcagacggt ctgagggcgt ttccggcggc tacgggataa
     9721 aacaggcaac aaaccacccg gaaaaccgcc ggaaacgcgc ccggtaaatt gtaacccgct
     9781 gcatgcctat gcacgcaact gcatggcggg ccgtttggtg taaaaacgat acgataagcc
     9841 gtgtttatgt aaatgaacgt ggccgtaaag agtgacaaat atacatgtaa cattttttac
     9901 tgacaggtca tcaacccgat gcgtttaata ccctttatcc tgctggcatt gtgcagcccc
     9961 ttactcctga cggcctgtaa gcattccacc acccagccgc cgaaaattta tactctggat
    10021 aagtcatcta aaatcagtag ctacgctgcg gccatcaaga cc
//
