LOCUS       NZ_CP087633             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02341 plasmid
            pDD02341-4, complete sequence.
ACCESSION   NZ_CP087633
VERSION     NZ_CP087633.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822725
            Assembly: GCF_023066685.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066685.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:10:42
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,896
            CDSs (total)                      :: 5,774
            Genes (coding)                    :: 5,609
            CDSs (with protein)               :: 5,609
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 59 of 165
            Pseudo Genes (incomplete)         :: 112 of 165
            Pseudo Genes (internal stop)      :: 21 of 165
            Pseudo Genes (multiple problems)  :: 27 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087633.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02341"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02341-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            559..717
                     /locus_tag="LMH53_RS29535"
     CDS             559..717
                     /locus_tag="LMH53_RS29535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1075..1401
                     /locus_tag="LMH53_RS29330"
                     /old_locus_tag="LMH53_29330"
     CDS             1075..1401
                     /locus_tag="LMH53_RS29330"
                     /old_locus_tag="LMH53_29330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            1427..1972
                     /locus_tag="LMH53_RS29335"
                     /old_locus_tag="LMH53_29335"
     CDS             1427..1972
                     /locus_tag="LMH53_RS29335"
                     /old_locus_tag="LMH53_29335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            2038..2388
                     /locus_tag="LMH53_RS29340"
                     /old_locus_tag="LMH53_29340"
     CDS             2038..2388
                     /locus_tag="LMH53_RS29340"
                     /old_locus_tag="LMH53_29340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(2458..2724)
                     /locus_tag="LMH53_RS29345"
                     /old_locus_tag="LMH53_29345"
     CDS             complement(2458..2724)
                     /locus_tag="LMH53_RS29345"
                     /old_locus_tag="LMH53_29345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2708..2932)
                     /locus_tag="LMH53_RS29350"
                     /old_locus_tag="LMH53_29350"
     CDS             complement(2708..2932)
                     /locus_tag="LMH53_RS29350"
                     /old_locus_tag="LMH53_29350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            complement(3599..3811)
                     /locus_tag="LMH53_RS29355"
                     /old_locus_tag="LMH53_29355"
     CDS             complement(3599..3811)
                     /locus_tag="LMH53_RS29355"
                     /old_locus_tag="LMH53_29355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3818..4327)
                     /locus_tag="LMH53_RS29360"
                     /old_locus_tag="LMH53_29360"
     CDS             complement(3818..4327)
                     /locus_tag="LMH53_RS29360"
                     /old_locus_tag="LMH53_29360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4362..5066)
                     /locus_tag="LMH53_RS29540"
                     /pseudo=""
     CDS             complement(<4362..5066)
                     /locus_tag="LMH53_RS29540"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5005..5349)
                     /locus_tag="LMH53_RS29370"
                     /old_locus_tag="LMH53_29370"
     CDS             complement(5005..5349)
                     /locus_tag="LMH53_RS29370"
                     /old_locus_tag="LMH53_29370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(5339..5515)
                     /locus_tag="LMH53_RS29375"
                     /old_locus_tag="LMH53_29375"
     CDS             complement(5339..5515)
                     /locus_tag="LMH53_RS29375"
                     /old_locus_tag="LMH53_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     Typing          complement(6..82)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.25"
                     /coverage="98.0"
                     /evalue="1.04e-21"
     Typing          271..668
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          complement(3484..5015)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggtgttagcg ggtttcgggg cgcagccctg aaccagtcac agcgtttacg ctgtgtatac
       61 tggcttacta tgttggcatg agagcacgat tcatgaaagt gcctcatgtg gcaggtgtga
      121 tatgtggctc aggagcgtcc gcaataccat gcacggacag atgaaatgac gcctcctcgc
      181 tcactcggta gctgcgctcc tgccgttcgg ctgcggcggg cgtgaccgac tcacggataa
      241 cgggataaag caaaggcgct caaatcagaa agggccggag aaaaggatgc ggcgttgccg
      301 tttttccata ggctccgcct cccctgacga gcatcacaga aatggacgct caagtcagag
      361 gaggacgaaa cccgacagga cttaaagatg ccggtgattt ctctccggcg gctccctcgt
      421 gcgctctcct gttccgaccc tgccgtttac cggatacctg ttccgccttt ttccctttcg
      481 ggaagcgtgg cgctttctca tagctcacgc tgctggtatc tcggctcggt ttaggtcgtt
      541 cgctccaagc tgggctgtat gcacgaaccc cccgttcagc ccgactgctg cgccctttcc
      601 ggtaactatc atcttgagtc caacccggac ggacacgcaa aaacgccact ggcagcagcc
      661 attggtaact ggatatatac gagagagata ttgagaggtc ttgaagtggg tgcctaactg
      721 cggctacact gaaagaacag ttttggtatc tgcggtctcg tatacccagt tacccggtta
      781 agcagttccc caactgactt aaccttcgat caaaccgcct ccccaggcgg ttttttcgtt
      841 tacagtgcaa gagattacgc gcagaaaaaa aggatctcaa gaagatcctt taaatgatcg
      901 tcagttctgt tatccccgga gctccatcca tcccacggaa aaacacccgc cactgatgcc
      961 ctcagcgcac tgcacatcat gtacaaacca ctccctccct ccttttttat tgcgtttctt
     1021 aatgcgcaat ggtagctttg tgcgcaacaa aaaacgcaat agcaaggggg ggtaatgaga
     1081 gaaagaacaa atatcatgct agatatagga ttaaagcaat ccttacaacg gctttcgaat
     1141 aaaaccaaaa tgagtctaag tgatcttatt aaccagagat tatcaggttc actttcagtt
     1201 gaagaacgca ttggcgaaaa gcctgaatgg atgagaaacc ttgatcccta tctcgccaat
     1261 cacttttcaa atattgagtt accgctccct tcaccattaa aagaatacca agaaaaaatg
     1321 gatgagatag attcaattat taatgacatg atggttctaa aggaatattt cagagctaag
     1381 ttttacgacg aaataaaata ggcacgctat acggaggcgg agaattatgt tttttaaacg
     1441 gtatttaacg gagaaggaaa tatacaaggc agcactccca attgttaggc ttcaagggag
     1501 agttaacact tcattacgtt ttttatccaa atactcagat gtaaacaaaa atggattaat
     1561 tgcattgcat cttaatttat tacattttat ctcatacaac atacacggtg tagagtctct
     1621 gaaaatcact caagcaggca taaacaaagc tgtacaacaa agccctcacg cagatgaaat
     1681 aaaaaatatt gcgcaggcaa taggacgcaa aggacctgag tacatcgaag gattaattag
     1741 aaacccatgg gggaagaaaa atgataatcc actaatattt ataatgagca ttgaagaaag
     1801 tataaaagaa acccttaact caggttatat gttatataga gtagctccca taactgaggg
     1861 tgaagcagct gctttcatgg atgttcttaa acaattatta ttatttgagg ataattttcg
     1921 cctttttggt agagacttgg tttataaaat agaaaaatta aaaatcagat aacaaagtat
     1981 ggtgaacgaa aaatgaaaga gaggatagtg aaatgtgtaa taccaagatt ctcaactatg
     2041 tattggttaa ttgcgttcct agcatttata ttccccggtc gatcttttgc tgacggttac
     2101 accgcttact accgatgtaa tggtactcag gaagcatctc ttactgttaa agataacaaa
     2161 ctaagtatgg ttgtaggtct ttctaagttt aataggcaac tggatactta tagattcaaa
     2221 gaaaagggaa tcgaagtagt aatgcagaga gctgcttctg atgataactc tgaggccata
     2281 atgtctttta ttatgcctga cgttaacaat atgaccttat tagttaaaaa taacggctac
     2341 attatcgtta agacaacctg ctggttaact aaaaccgaca aacagtagaa cacgtagtta
     2401 gtcgagtatt taccataata cctgttgtcg gcatcaaaaa agcccgcata gcgggcttca
     2461 gtcgtaaact tcgcggcgat gtccaatcgt ggcggcaagg ataacaagct cgtcatctcg
     2521 gatctcacac agaacgcgat aatctcccac ccgatagcgc cagaactcgc ccagctcacc
     2581 tttgagcggc ttccctgact ggcgaggatc ggcagcaact gcaatgcgta aactcataaa
     2641 atccacaatc cgtcgtgcgt tctgtttgtc catcttgcgt aacgatttga gcgcccgatc
     2701 ggaatagtta atcgtccaga ccaagtgact tcctcacgtc ttcagacgaa tgcactgctt
     2761 catcaccacg gcgaacgcgt gcagcagttt ctgctgaaag ataataatcc tccaggtctt
     2821 ccagatgcgc cagtattgct tcccgcgcat aaaacgtctt tgttctgccg gtttgcttcg
     2881 ccagcgagtc cagacgggac tcaatctcat cagataaccg gatagcaagc atataaacct
     2941 cccgaacacc atttgctata catgtatatc aacagtacca caatcaacct aaaagaacaa
     3001 aaaataatct tgttagcccc tcttgataat ccgtaacgcg gtcaatatat ctagcttgag
     3061 ggctttcctc ttaactattg aaaggataac gattatgggt cttaagccgg gtccgaaacg
     3121 aattgccgat tcaacagggg aacctgataa acgccagcgt gacaataaaa aaacgccggg
     3181 aaatactgac aaattgaaac ccagcaaatc ttcaaaaaaa taagtcagta gttaaaagcc
     3241 ctccggggct tttttgtatc tgccgttcac gctgattaaa cataacaccc gttgcccgca
     3301 ccgccccgga cgggttcgcc gcgaacgggg tggcagtcag gtaaaaccgg caacaaacca
     3361 cccgataaac cgccccgttc gcgacagata aattttacct ggctgcatag ccatgcagcc
     3421 aggtgaatga catccggttt cagcgtaaat gcccgatgag aaccctgttt tcctgcattt
     3481 cagcccagcg acggaccgtc atagcttcgc tgccgggaac gcgcttccgt cagttccttt
     3541 tcgtgctgcc gttgctcgtg cgcccgttct gcggccacgg ccatctcatg acgctgcact
     3601 accggctcaa acgctgcagt tgcccgctca agcgttccac ctgctgactc aagcgcgtca
     3661 cgcgctcgct cagcgccgcg ttctcccgtt gcataagccc tgacattttc cgccattccc
     3721 cgaaggcgct ctcccactcg tccagccttt tcgagtagtc ctgctgtagc tgctctaatg
     3781 cgctcagcaa ctgtttttcc agctcggtca tcggcactta ctccttcacc agtctcagcg
     3841 cgttctgctt cgaccgcccg ttcccgaccg tccagctctg cgtcctgtct acggccgtcc
     3901 ccttcggcag caccagaaaa cgtccgttgc tgtcttccag gtacgtcacg ccccaggtct
     3961 tcgcgttcag cttcgacagc gctgcgttct gccggtcgat ttcggccaga tttgacgcta
     4021 tcaagctccc ctgataccac aacaccccgc tcaggctggc gaacagcagt cctgacacca
     4081 tcgtgatggt cagccatgtc cgcccgacca tacgcatcac gctcaggcgg ttcgactgca
     4141 tggccgcgct cataccctgc tcgtgggcgc ggatggcggc gctgattctc ttcccgcttt
     4201 cgctcagttc cgctctgaca gacttctcgt gctcgctgaa tgcggctttc agcatttcgc
     4261 cggtactctg ctgctgcgct ttcgatttct gctctaagtc cttcgccagc gttaaaagac
     4321 tgttcataga tggctccctt aagtcggatg tttcgccctc cgtccgggtc ggcaatgctg
     4381 atgctgctct ttgtggtgcg cacgacctca aaacctgcgc tttccagcgc ctcagtgacg
     4441 tcctgacgcg ttttaagctc ccctgacgag gcgagggcca gtaagccccg cgtaatagcc
     4501 tctgcggctt cctgcttcgt tttgggtaac gaagatggcg tcaccagcgc gcgccggttc
     4561 tccggtgcgt tcgggtcgtg cagccccagt cgcccgttca ccacggtctg ccacgcatcg
     4621 atgcgcggac ggtctgcccg gtcgtagtac ggctggagac gcctgccggt cagcagttcc
     4681 gtgttcggta ccagaaaatt cagttccagc cgccccttgt cccggtgttc aacccacagc
     4741 acgctgtact ggtctttatc gagtccgggc atcagaaccc gctcgaagct cgccatcagc
     4801 ttttcacgct ggccgggcgg taaatcctgt tcagcaaaag acaggacgcc ggaggtgtac
     4861 tttttggcgt agggcgaggc gtcgataagc tcccggacct cctccggctt tccctgcagg
     4921 acgatggccc cgtcgcgctg gcggtctttc cccagcagat aatccaccgg cccggcaccg
     4981 ccgcctcgcc ctcgcggatg aaacttaacg atcatcgtct gccccctttt ccagcacggc
     5041 atgccgcagc cgctcgagtt cggcatcaat ggccatcagc gcggccacca cctgaacccg
     5101 gtcagcgcct gaccactggc cgccgttaat tttccgggcg atctggttga ggttgttccc
     5161 catcccggca agctggcgca gcaaaaccgg atcgatggac ggcagccgca acgaccgcgc
     5221 cgggcgcgta tcgagacagg tttcccgcat ccacaccgcc agttgccttc cattgcagcg
     5281 ctccagcagc tgctggtact ccccatcggt cacccggata cctaaaaaac gggtacgctt
     5341 aaccgccatc ctctcctccg atatccgcaa aatgtgaccg ggctgaacgc tcaagctctg
     5401 ccatctgctc ccggcggtaa tcttcctcct gtgcctgacg catcagattt gccatcactg
     5461 ccggatgcca ggatcttttt tttacgacga gcggcttctt cttcggcttc tgcattatcc
     5521 ctccttcgca aacgccggac ggcacggaaa agttttccgg gtcgctccgg cgttggacaa
     5581 gcgccagcgc gtcagt
//
