LOCUS       NZ_CP087627             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02280 plasmid
            pDD02280-4, complete sequence.
ACCESSION   NZ_CP087627
VERSION     NZ_CP087627.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822726
            Assembly: GCF_023066665.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066665.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 03:58:05
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,950
            CDSs (total)                      :: 5,828
            Genes (coding)                    :: 5,640
            CDSs (with protein)               :: 5,640
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 188
            CDSs (without protein)            :: 188
            Pseudo Genes (ambiguous residues) :: 0 of 188
            Pseudo Genes (frameshifted)       :: 67 of 188
            Pseudo Genes (incomplete)         :: 128 of 188
            Pseudo Genes (internal stop)      :: 25 of 188
            Pseudo Genes (multiple problems)  :: 32 of 188
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087627.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02280"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02280-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(316..474)
                     /locus_tag="LMH50_RS29805"
     CDS             complement(316..474)
                     /locus_tag="LMH50_RS29805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            1114..1290
                     /locus_tag="LMH50_RS29570"
                     /old_locus_tag="LMH50_29570"
     CDS             1114..1290
                     /locus_tag="LMH50_RS29570"
                     /old_locus_tag="LMH50_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            1280..1624
                     /locus_tag="LMH50_RS29575"
                     /old_locus_tag="LMH50_29575"
     CDS             1280..1624
                     /locus_tag="LMH50_RS29575"
                     /old_locus_tag="LMH50_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            1833..>2267
                     /locus_tag="LMH50_RS29580"
                     /old_locus_tag="LMH50_29580"
                     /pseudo=""
     CDS             1833..>2267
                     /locus_tag="LMH50_RS29580"
                     /old_locus_tag="LMH50_29580"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2302..2811
                     /locus_tag="LMH50_RS29585"
                     /old_locus_tag="LMH50_29585"
     CDS             2302..2811
                     /locus_tag="LMH50_RS29585"
                     /old_locus_tag="LMH50_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            2818..3030
                     /locus_tag="LMH50_RS29590"
                     /old_locus_tag="LMH50_29590"
     CDS             2818..3030
                     /locus_tag="LMH50_RS29590"
                     /old_locus_tag="LMH50_29590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            3697..3921
                     /locus_tag="LMH50_RS29595"
                     /old_locus_tag="LMH50_29595"
     CDS             3697..3921
                     /locus_tag="LMH50_RS29595"
                     /old_locus_tag="LMH50_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            3905..4171
                     /locus_tag="LMH50_RS29600"
                     /old_locus_tag="LMH50_29600"
     CDS             3905..4171
                     /locus_tag="LMH50_RS29600"
                     /old_locus_tag="LMH50_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4241..4591)
                     /locus_tag="LMH50_RS29605"
                     /old_locus_tag="LMH50_29605"
     CDS             complement(4241..4591)
                     /locus_tag="LMH50_RS29605"
                     /old_locus_tag="LMH50_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     gene            complement(4657..5202)
                     /locus_tag="LMH50_RS29610"
                     /old_locus_tag="LMH50_29610"
     CDS             complement(4657..5202)
                     /locus_tag="LMH50_RS29610"
                     /old_locus_tag="LMH50_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(5228..5554)
                     /locus_tag="LMH50_RS29615"
                     /old_locus_tag="LMH50_29615"
     CDS             complement(5228..5554)
                     /locus_tag="LMH50_RS29615"
                     /old_locus_tag="LMH50_29615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     Typing          949..1028
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(366..763)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          1615..3146
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccattgcgca ttaagaaacg caataaaaaa ggagggaggg agtggtttgt acatgatgtg
       61 cagtgcgctg agggcatcag tggcgggtgt ttttccgtgg gatggatgga gctccgggga
      121 taacagaact gacgatcatt taaaggatct tcttgagatc ctttttttct gcgcgtaatc
      181 tcttgcactg taaacgaaaa aaccgcctgg ggaggcggtt tgatcgaagg ttaagtcagt
      241 tggggaactg cttaaccggg taactgggta tacgagaccg cagataccaa aactgttctt
      301 tcagtgtagc cgcagttagg cacccacttc aagacctctc aatatctctc tcgtatatat
      361 ccagttacca atggctgctg ccagtggcgt ttttgcgtgt ccgtccgggt tggactcaag
      421 atgatagtta ccggaaaggg cgcagcagtc gggctgaacg gggggttcgt gcatacagcc
      481 cagcttggag cgaacgacct aaaccgagcc gagataccag cagcgtgagc tatgagaaag
      541 cgccacgctt cccgaaaggg aaaaaggcgg aacaggtatc cggtaaacgg cagggtcgga
      601 acaggagagc gcacgaggga gccgccggag agaaatcacc ggcatcttta agtcctgtcg
      661 ggtttcgtcc tcctctgact tgagcgtcca tttctgtgat gctcgtcagg ggaggcggag
      721 cctatggaaa aacggcaacg ccgcatcctt ttctccggcc ctttctgatt tgagcgcctt
      781 tgctttatcc cgttatccgt gagtcggtca cgcccgccgc agccgaacgg caggagcgca
      841 gctaccgagt gagcgaggag gcgtcatttc atctgtccgt gcatggtatt gcggacgctc
      901 ctgagccaca tatcacacct gccacatgag gcactttcat gaatcgtgct ctcatgccaa
      961 catagtaagc cagtatacac agcgtaaacg ctgtgactgg ttcagggctg cgccccgaaa
     1021 cccgctaaca ccactgacgc gctggcgctt gtccaacgcc ggagcgaccc ggaaaacttt
     1081 tccgtgccgt ccggcgtttg cgaaggaggg ataatgcaga agccgaagaa gaagccgctc
     1141 gtcgtaaaaa aaagatcctg gcatccggca gtgatggcaa atctgatgcg tcaggcacag
     1201 gaggaagatt accgccggga gcagatggca gagcttgagc gttcagcccg gtcacatttt
     1261 gcggatatcg gaggagagga tggcggttaa gcgtacccgt tttttaggta tccgggtgac
     1321 cgatggggag taccagcagc tgctggagcg ctgcaatgga aggcaactgg cggtgtggat
     1381 gcgggaaacc tgtctcgata cgcgcccggc gcggtcgttg cggctgccgt ccatcgatcc
     1441 ggttttgctg cgccagcttg ccgggatggg gaacaacctc aaccagatcg cccggaaaat
     1501 taacggcggc cagtggtcag gcgctgaccg ggttcaggtg gtggccgcgc tgatggccat
     1561 tgatgccgaa ctcgagcggc tgcggcatgc cgtgctggaa aagggggcag acgatgatcg
     1621 ttaagtttca tccgcgaggg cgaggcggcg gtgccgggcc ggtggattat ctgctgggga
     1681 aagaccgcca gcgcgacggg gccatcgtcc tgcagggaaa gccggaggag gtccgggagc
     1741 ttatcgacgc ctcgccctac gccaaaaagt acacctccgg cgtcctgtct tttgctgaac
     1801 aggatttacc gcccggccag cgtgaaaagc tgatggcgag cttcgagcgg gttctgatgc
     1861 ccggactcga taaagaccag tacagcgtgc tgtgggttga acaccgggac aaggggcggc
     1921 tggaactgaa ttttctggta ccgaacacgg aactgctgac cggcaggcgt ctccagccgt
     1981 actacgaccg ggcagaccgt ccgcgcatcg atgcgtggca gaccgtggtg aacgggcgac
     2041 tggggctgca cgacccgaac gcaccggaga accggcgcgc gctggtgacg ccatcttcgt
     2101 tacccaaaac gaagcaggaa gccgcagagg ctattacgcg gggcttactg gccctcgcct
     2161 cgtcagggga gcttaaaacg cgtcaggacg tcactgaggc gctggaaagc gcaggttttg
     2221 aggtcgtgcg caccacaaag agcagcatca gcattgccga cccggacgga gggcgaaaca
     2281 tccgacttaa gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag
     2341 aaatcgaaag cgcagcagca gagtaccggc gaaatgctga aagccgcatt cagcgagcac
     2401 gagaagtctg tcagagcgga actgagcgaa agcgggaaga gaatcagcgc cgccatccgc
     2461 gcccacgagc agggtatgag cgcggccatg cagtcgaacc gcctgagcgt gatgcgtatg
     2521 gtcgggcgga catggctgac catcacgatg gtgtcaggac tgctgttcgc cagcctgagc
     2581 ggggtgttgt ggtatcaggg gagcttgata gcgtcaaatc tggccgaaat cgaccggcag
     2641 aacgcagcgc tgtcgaagct gaacgcgaag acctggggcg tgacgtacct ggaagacagc
     2701 aacggacgtt ttctggtgct gccgaagggg acggccgtag acaggacgca gagctggacg
     2761 gtcgggaacg ggcggtcgaa gcagaacgcg ctgagactgg tgaaggagta agtgccgatg
     2821 accgagctgg aaaaacagtt gctgagcgca ttagagcagc tacagcagga ctactcgaaa
     2881 aggctggacg agtgggagag cgccttcggg gaatggcgga aaatgtcagg gcttatgcaa
     2941 cgggagaacg cggcgctgag cgagcgcgtg acgcgcttga gtcagcaggt ggaacgcttg
     3001 agcgggcaac tgcagcgttt gagccggtag tgcagcgtca tgagatggcc gtggccgcag
     3061 aacgggcgca cgagcaacgg cagcacgaaa aggaactgac ggaagcgcgt tcccggcagc
     3121 gaagctatga cggtccgtcg ctgggctgaa atgcaggaaa acagggttct catcgggcat
     3181 ttacgctgaa accggatgtc attcacctgg ctgcatggct atgcagccag gtaaaattta
     3241 tctgtcgcga acggggcggt ttatcgggtg gtttgttgcc ggttttacct gactgccacc
     3301 ccgttcgcgg cgaacccgtc cggggcggtg cgggcaacgg gtgttatgtt taatcagcgt
     3361 gaacggcaga tacaaaaaag ccccggaggg cttttaacta ctgacttatt tttttgaaga
     3421 tttgctgggt ttcaatttgt cagtatttcc cggcgttttt ttattgtcac gctggcgttt
     3481 atcaggttcc cctgttgaat cggcaattcg tttcggaccc ggcttaagac ccataatcgt
     3541 tatcctttca atagttaaga ggaaagccct caagctagat atattgaccg cgttacggat
     3601 tatcaagagg ggctaacaag attatttttt gttcttttag gttgattgtg gtactgttga
     3661 tatacatgta tagcaaatgg tgttcgggag gtttatatgc ttgctatccg gttatctgat
     3721 gagattgagt cccgtctgga ctcgctggcg aagcaaaccg gcagaacaaa gacgttttat
     3781 gcgcgggaag caatactggc gcatctggaa gacctggagg attattatct ttcagcagaa
     3841 actgctgcac gcgttcgccg tggtgatgaa gcagtgcatt cgtctgaaga cgtgaggaag
     3901 tcacttggtc tggacgatta actattccga tcgggcgctc aaatcgttac gcaagatgga
     3961 caaacagaac gcacgacgga ttgtggattt tatgagttta cgcattgcag ttgctgccga
     4021 tcctcgccag tcagggaagc cgctcaaagg tgagctgggc gagttctggc gctatcgggt
     4081 gggagattat cgcgttctgt gtgagatccg agatgacgag cttgttatcc ttgccgccac
     4141 gattggacat cgccgcgaag tttacgactg aagcccgcta tgcgggcttt tttgatgccg
     4201 acaacaggta ttatggtaaa tactcgacta actacgtgtt ctactgtttg tcggttttag
     4261 ttaaccagca ggttgtctta acgataatgt agccgttatt tttaactaat aaggtcatat
     4321 tgttaacgtc aggcataata aaagacatta tggcctcaga gttatcatca gaagcagctc
     4381 tctgcattac tacttcgatt cccttttctt tgaatctata agtatccagt tgcctattaa
     4441 acttagaaag acctacaacc atacttagtt tgttatcttt aacagtaaga gatgcttcct
     4501 gagtaccatt acatcggtag taagcggtgt aaccgtcagc aaaagatcga ccggggaata
     4561 taaatgctag gaacgcaatt aaccaataca tagttgagaa tcttggtatt acacatttca
     4621 ctatcctctc tttcattttt cgttcaccat actttgttat ctgattttta atttttctat
     4681 tttataaacc aagtctctac caaaaaggcg aaaattatcc tcaaataata ataattgttt
     4741 aagaacatcc atgaaagcag ctgcttcacc ctcagttatg ggagctactc tatataacat
     4801 ataacctgag ttaagggttt cttttatact ttcttcaatg ctcattataa atattagtgg
     4861 attatcattt ttcttccccc atgggtttct aattaatcct tcgatgtact caggtccttt
     4921 gcgtcctatt gcctgcgcaa tattttttat ttcatctgcg tgagggcttt gttgtacagc
     4981 tttgtttatg cctgcttgag tgattttcag agactctaca ccgtgtatgt tgtatgagat
     5041 aaaatgtaat aaattaagat gcaatgcaat taatccattt ttgtttacat ctgagtattt
     5101 ggataaaaaa cgtaatgaag tgttaactct cccttgaagc ctaacaattg ggagtgctgc
     5161 cttgtatatt tccttctccg ttaaataccg tttaaaaaac ataattctcc gcctccgtat
     5221 agcgtgccta ttttatttcg tcgtaaaact tagctctgaa atattccttt agaaccatca
     5281 tgtcattaat aattgaatct atctcatcca ttttttcttg gtattctttt aatggtgaag
     5341 ggagcggtaa ctcaatattt gaaaagtgat tggcgagata gggatcaagg tttctcatcc
     5401 attcaggctt ttcgccaatg cgttcttcaa ctgaaagtga acctgataat ctctggttaa
     5461 taagatcact tagactcatt ttggttttat tcgaaagccg ttgtaaggat tgctttaatc
     5521 ctatatctag catgatattt gttctttctc tcattacccc cccttgctat tgcgtttttt
     5581 gttgcgcaca aagcta
//
