LOCUS       NZ_CP087622            10060 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02162 plasmid
            pDD02162-4, complete sequence.
ACCESSION   NZ_CP087622
VERSION     NZ_CP087622.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822727
            Assembly: GCF_023066645.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066645.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:00:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,485
            CDSs (total)                      :: 5,362
            Genes (coding)                    :: 5,213
            CDSs (with protein)               :: 5,213
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 149
            CDSs (without protein)            :: 149
            Pseudo Genes (ambiguous residues) :: 0 of 149
            Pseudo Genes (frameshifted)       :: 63 of 149
            Pseudo Genes (incomplete)         :: 95 of 149
            Pseudo Genes (internal stop)      :: 20 of 149
            Pseudo Genes (multiple problems)  :: 24 of 149
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087622.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02162"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02162-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(1134..1283)
                     /locus_tag="LMH58_RS27280"
                     /old_locus_tag="LMH58_27280"
     CDS             complement(1134..1283)
                     /locus_tag="LMH58_RS27280"
                     /old_locus_tag="LMH58_27280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1368..1625)
                     /locus_tag="LMH58_RS27285"
                     /old_locus_tag="LMH58_27285"
     CDS             complement(1368..1625)
                     /locus_tag="LMH58_RS27285"
                     /old_locus_tag="LMH58_27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1635..3320)
                     /locus_tag="LMH58_RS27290"
                     /old_locus_tag="LMH58_27290"
     CDS             complement(1635..3320)
                     /locus_tag="LMH58_RS27290"
                     /old_locus_tag="LMH58_27290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            3647..3892
                     /locus_tag="LMH58_RS27295"
                     /old_locus_tag="LMH58_27295"
     CDS             3647..3892
                     /locus_tag="LMH58_RS27295"
                     /old_locus_tag="LMH58_27295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            4166..4537
                     /gene="tolA"
                     /locus_tag="LMH58_RS27300"
                     /old_locus_tag="LMH58_27300"
     CDS             4166..4537
                     /gene="tolA"
                     /locus_tag="LMH58_RS27300"
                     /old_locus_tag="LMH58_27300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            4534..4899
                     /locus_tag="LMH58_RS27305"
                     /old_locus_tag="LMH58_27305"
     CDS             4534..4899
                     /locus_tag="LMH58_RS27305"
                     /old_locus_tag="LMH58_27305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            5074..5352
                     /locus_tag="LMH58_RS27310"
                     /old_locus_tag="LMH58_27310"
     CDS             5074..5352
                     /locus_tag="LMH58_RS27310"
                     /old_locus_tag="LMH58_27310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            5340..5654
                     /locus_tag="LMH58_RS27315"
                     /old_locus_tag="LMH58_27315"
     CDS             5340..5654
                     /locus_tag="LMH58_RS27315"
                     /old_locus_tag="LMH58_27315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            5818..6246
                     /locus_tag="LMH58_RS27320"
                     /old_locus_tag="LMH58_27320"
     CDS             5818..6246
                     /locus_tag="LMH58_RS27320"
                     /old_locus_tag="LMH58_27320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            6272..6451
                     /locus_tag="LMH58_RS27325"
                     /old_locus_tag="LMH58_27325"
     CDS             6272..6451
                     /locus_tag="LMH58_RS27325"
                     /old_locus_tag="LMH58_27325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(6478..7008)
                     /locus_tag="LMH58_RS27330"
                     /old_locus_tag="LMH58_27330"
     CDS             complement(6478..7008)
                     /locus_tag="LMH58_RS27330"
                     /old_locus_tag="LMH58_27330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7015..7746)
                     /gene="mobC"
                     /locus_tag="LMH58_RS27335"
                     /old_locus_tag="LMH58_27335"
     CDS             complement(7015..7746)
                     /gene="mobC"
                     /locus_tag="LMH58_RS27335"
                     /old_locus_tag="LMH58_27335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(7746..9710)
                     /locus_tag="LMH58_RS27340"
                     /old_locus_tag="LMH58_27340"
     CDS             complement(7746..9710)
                     /locus_tag="LMH58_RS27340"
                     /old_locus_tag="LMH58_27340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8320
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          453..1031
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(7019..7746)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 ggtcaggcgt gagcctgacg ggggccgggt gaatttctgg cgtggccggc gtccagccgg
       61 acagagccag aaagagggga aaagaccgtg cgtcggtggc gtccagccag cgtaagcaag
      121 gtcttttccc ctgaagtcag ccacgcccca gcggggatga cttgcatttc tccgcaagga
      181 gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga catggttcac
      241 ctatcctgat aattgatcgt cagggaatag aaagacttaa tcaggggaca atatcccaca
      301 tcagcgcgtc cggtgaccgt cttgcaggtg ccggggaaat tgcgcctcct cgctcactcg
      361 gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca aagttaccca
      421 cagattccgt ggataagcag gggactaaca tgtgaggcaa aacggcaggg ccgcgccggt
      481 ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac gctttttcgg
      541 tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg aaacccgaca
      601 ggacttaaag atccccaccg tttccggtag gtcgctccct cttgcgctct cctgttccga
      661 ccctgccgtt taccggatac ctgtccgccg ttctcccttg cgggaagtgt ggcgctttct
      721 catagctcac acactggtat ctcggctcgg tgtaggtcgt tcgctccaag ctgggctgta
      781 agcaagaact ccccgttcag cccgactgct gcgccttatc cggtaactgt tcacttgagt
      841 ccaacccggg aaagcacgca aaacgccact ggcagcagcc attggtaact gggagttcgc
      901 agaggatttg tttagctaaa cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac
      961 actggaagga cagatttggt tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt
     1021 tccccaactg acttaacctt cgatcaaacc acctccccag gtggtttttt cgtttacagg
     1081 gcaaaagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctactga
     1141 accgcgatcc ccgtcagttc agaggaggac gatggtgcca ccgttccgcc ctgaacatcc
     1201 cggatataat ttgcctgaca tgcggccaga agaaagccag caacaatcaa tataaaaaga
     1261 aaaatagtct ttaccttttt catatacttt ccctgtattt aaagactccg gcattcatcc
     1321 ctgaacaccg gaagtcagta tttcaaatca tccttgataa ttttttgtta ccagttaccg
     1381 tcccgataat caaaagctat ataacaatta tccacatcag gttttatttg atttttaaaa
     1441 aatggctgta gtaaaggaac ccattctttc ttcaaatcaa actcaccatt attaatatta
     1501 tcctttaaag gcattcccaa gctttcaatg acagaaccat cgtcaccaaa gtctttagaa
     1561 tactcttccc ctttaaactc ttcagttttc ttatcaaacc atgtcaaacg taatttaagc
     1621 cccatattta cctctcaaag atatttttta atgttccgtt ttggatctgg ccctttaacc
     1681 tgcttacccg ttttgggatc aaatgcgcct aggtgttcgc catcactggc acgataccct
     1741 tcaagctcac cgtgctggga atcccattca taaattttac gttttttatc gccataccat
     1801 cgggcccgct taccaccacc accttgttta ggtgttttag gatcacctcg ctttaactct
     1861 ccaagtccct ttatgtcttc tgttttaggt actggatgat aatcatggcc gtagtcttta
     1921 gtccccttgc gaggcttgtt cttttcctca tttaacttat tttctgcatc cttcgctttt
     1981 ttctcctttt gtttccggct ctccatcgcc ccggtaagtg cagccttatc tctggataag
     2041 gattcattca ttgcgtttaa agatgcctga gccgctttta atttctcatt ctgattctga
     2101 atatccttat caagatagcg aacctgatcc tgccacccgg agccatattc atgaggcggg
     2161 ttataagtca caaaatcatc tttatttgcc tctgcctctt ttaccttttt atcggcttcc
     2221 tgaacagcag cccttgcacc ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag
     2281 gtcgcaatat ttttatcttc ggcatccagc tcagctttcg ctttatcata gtctcttttc
     2341 agcccctctt ccggatgagc ggcgtcccat gcctgctggc gacgcttttc ttcctcctga
     2401 cgctgtttta cctgtgccgg ggtaagaaca tctgtcaccg acacataaac cggcttctgt
     2461 ccggtctctt tcgggaaacg aataacggcc tcacgggagt taccaccggc tgtaaaacca
     2521 gccggacgtg aatcaccttt ttcagcaaca atgccttttg gcggggcttt cgctgtcgga
     2581 acacctttcg gtacgctcac ctgcagagac gggagacccg gaatcgacac actgaatacc
     2641 cccggacgtt ttgtcggttt cgcatccacc acagggacac tcattggccg acctgcgaca
     2701 accgcaatat gctgccgctc atccttcacc acatccacaa cccgttgtct gacacgaacc
     2761 gtcgcctggt ccagtggtaa agtacttgcc ggagtctccg tcaccgcatc ggccggtaat
     2821 gacgtcataa tttttgacat cattttcgga tcatcttttg caatctcaga aggcagcaca
     2881 ccgtagatcg caatccccca cagaccaaac ttaaacggcc ccttcagggc agccagcaca
     2941 tcagcaacgg cggccgacaa cgcatcgccg gaaactgata aagccagtcc ttcagccccc
     3001 ggagtagcca gggcaggcag accgaaggcc atcgcagtgg ccgaagactg tccgccacca
     3061 gagctaccgc tatgattgcc gctgttacca ccattaccac cgggtttact cccgccgtta
     3121 ccaaactccc ccggattata gttatgaata tctccattcg gcgtatgcgt cgtaccccag
     3181 cccggcccgc tgtttggatc cgtaccacca cctgatgaag ggccaccttt accagacgta
     3241 ccgttcacat tcccactggc ctgaccacca ttatgtccca gacctgaatt acccggacct
     3301 cgaccgtctc caccactcat accgttttcc tctttgacat ttaaaacaat aaattaaagc
     3361 caacatactg tacatataca cagtataatt attaccttac tcctcccgtt ttcaggtgtc
     3421 aatatatgag attagttctc atcttcaggc acaaaaaagc gcccggagcc acctgtaagc
     3481 agatcgcgtc ttttcccgaa aatttctcat ggcaacaaat tttattattt aagacattca
     3541 tcacacttac atttcagatg gtatagacac atctgaaagc taactggcat gaggagagga
     3601 cctgcggtta aactgttcgg gaaacctgag acgcccggag gccactatga cgcacctgaa
     3661 actgactccc gctgaagaaa agcgcctggc ggacgccttt gtcgctcagg cactggccat
     3721 gggcctgaaa gggaacaaaa cgctgccaaa ggcggtaaaa caacaggtgc gggaaagggt
     3781 gcttcgtgcc attcaggacg aacgtgaagc caggcgggcg caggaaatgc gtcgggagaa
     3841 atacgaggct acggaaacca ccttcacctg gcagccacag cgccgccggt aaggggtatt
     3901 taccataacg tcgcattgcc cgcaccgctc agacggtctg agggcgtttc cggcggctac
     3961 gggataaaac aggcaacaaa ccacccggaa aaccgccgga aacgcgcccg gtaaattgta
     4021 acccgctgca tgcctatgca cgcaactgca tggcgggccg tttggtgtaa aaacgatacg
     4081 ataagccgtg tttatgtaaa tgaacgtggc cgtaaagagt gacaaatata catgtaacat
     4141 tttttactga caggtcatca acccgatgcg tttaataccc tttatcctgc tggcattgtg
     4201 cagcccctta ctcctgacgg cctgtaagca ttccaccacc cagccgccga aaatttatac
     4261 tctggataag tcatctaaaa tcagtagcta cgctgcggcc atcaagaccg ctgttgaaat
     4321 gcagatgttt gaaccagagg cctatgcggg aaaaatgtgc accgtgcgga tatccataca
     4381 acgtgatggc actctgaaca gcgccattgc tgaaggagga gatccgaaac tctgtaaagc
     4441 agcaatatca gccgttaccc acgccaaaat tccgccggca ccggatgaaa atacctggca
     4501 aatattcaaa aacgcccctc tggatttcag accatgaaag cttttactct gacagtgctg
     4561 gcggccatgt tcctcactcc ggtacatgcg ataccactaa aacccgtaac cacactgcag
     4621 ccaacctacc cggtacaggc cgcagcaaaa cgcatcgaag gtcaggtgac cgccacgttt
     4681 gatattctgg acgatggacg agtggacaac gtgtcgctta ccggctgtca cctttttttt
     4741 cgggaaacgc tgacggcaat gaggcactgg ctatatcccg cagggcaccc ggcaatgcga
     4801 caggttataa attttgaatt cagtctgaaa aatgacggct tacaggatcc gcgaccagtc
     4861 accacccaaa agctcaatga tgatggttgc cgccagtaac aggtccttta acccgcaggc
     4921 tgcaatagtc ctggatgatg agtgagcctg tagcagccct gattagtgga agcgaagctg
     4981 aaaaatgggc agcaaaagcc gtatttctgg caaatagcgc cgccacggat taaagtagtc
     5041 atgtgtaata catatgacta caaaggggct tttatgtcat cagcaacatc catcaggctc
     5101 gatgaagaac ttaaggaccg gctgaaaacg ctggccgatg accggcaccg ctcagcgcac
     5161 gcgctaatgc tggaggcaat caccgaatac atcgaccggg aagagaagcg cagccagtac
     5221 ctgcgggacg gacaggccgc atggcagcac tatcaggaaa cgggtctgca ccttactgcg
     5281 gaagaagcgg aagcctggat aagtacctgg ggaactgaga acgagcagga tgctccgcca
     5341 tgccacaggt aacgatttcc gcgctggcac agcgcgattt acaacgcctt caggactttc
     5401 tcaaaaccaa aaatcggctg gcagccagaa aaggaggtga ggtgatcgtc cgggctatcc
     5461 agcaactgaa aacattgcca gacattggcc gcccggtgcc gtttctgccg ctggaatata
     5521 aggaactggt gatcgggttt ggcgacagtg gctatgtgat gctctaccgc cacgaccggg
     5581 aaatggacca gattgtgatc gtcaccgtca ggcatcagaa agaatccggg tatccggggg
     5641 cagacagcct ctaataaccg atgacggggg cgcaattcat tgcgtggccg tcgccggttt
     5701 gacaagcgca gcgcgtcaga aaaccacatc ctgaacggaa aaacgggtaa aattcagcga
     5761 tacatttacc gtgagaaatt acacaccttt cacacccgat aaaatcaggg gtatacagtg
     5821 gcgaaaacat tgctcaacct ttcacaggca gcacaagccg cgggcattac cagaagaacg
     5881 ctatacaatc atgtaaaaca gggaaaggtt accgtgtcac gtgacgggaa aaacaaccct
     5941 gtcgtggatg tgtcagaact gatccgtgta tacggaaatg tgaatatccc tgagaaacaa
     6001 attcccggca tttcacaccg tgaaaataca caaaaaaatt tcccacagga acagcttctg
     6061 gcaatgcaaa aggaactggc cgatctccgg caggctgtca ccctgatgct ggaagataaa
     6121 acatcccggg aagaagaacg ccgccagcac gatgatgagc gccggaaact ccaggccgaa
     6181 gtggacagat tgacaacaga actgacacag aagaaaaaac gcttctggtc cggctggttt
     6241 agctgagaac ttaccgaagg gaatggagaa catgacgaag caggaaaaaa ctgccctcaa
     6301 tatggctaaa ttcattaagg atcagtcgct gctgttgctg gaaaagctga acgagctgga
     6361 catggatacc ccggccgaca tgtgcgaacg gcttcacgag gatgcggaaa cactctggca
     6421 ggtgatgcgg gataagctcg gggaagaata acagcaccgg ccatgccggt gctggcgtta
     6481 ttttttgccg aacggccacc accggcgggc gggtttgtcc tccggcagtt caggcaccgg
     6541 cacgttaaaa tagcggctgt tatattcccg catcagctcc gctttcagct tctcgttcac
     6601 cgcgctttcc ccgtgccgct cgctgaccac ctcaatcatc gcgttaatct gctccggtga
     6661 gcacttcccg gcatcaacca cctgcacacc gttctcgatg gcgaaccgct ccacctttcg
     6721 atagaggtaa tcacacagct tcattcctgt gggtttgtcc cgttcccatt gcggattttc
     6781 accggtgaaa aagtacgtgg ctttttcgtc gctgttgagc cgctcgtcat acatcccgat
     6841 ggatttcatc atcgccagat gctgggcccg gtcgacaaaa cagaaaacat acgccccggt
     6901 ctgccaggcg agcaccacca tgtcttccag atgctcgctg gttggcataa tgcagcgctc
     6961 gccgtctttg tcttccacgt cctcaataaa taccatgtac agcgccataa tccctcactc
     7021 cgttgtcata aatttaaagc acgcgaacag cctctcccgg tccagctcca ccgttttgcg
     7081 ggcctcgccg gtctgtggat tcacgatttc atccaccgcc agaatcgccc gctgtacccg
     7141 gacaagcgtc ggccggtcag ggcagatgta ccagatttca tcgatgccgt tcgcccggcg
     7201 gttgaacaga tacccggaca ccacttcctg ataccgcctg cggctcttga cggtgcgctc
     7261 tacctcaaat gccaccgttc tgccgtccag ctccgccagc gcgtccggca ctttcatccc
     7321 tttcagggcc atacggtgca ggtaccgcca gccggtggcc ccccggccct ccatggagag
     7381 gcgcagggac tgcacggcca gccggtgcgg cagctgcgcc gccgacaccc ttgacggctg
     7441 aaacggaata acgtccgtca gcggctcgtc ctcgtcgaac gagaacgcca ccccgtgcgg
     7501 cgtcagcccc cacacgctga cctgacggcc gtcaaccggg tatttcgccc ggaccagcag
     7561 ccctttcgcc accagactgt ccaggaagcg gtaaaccggc tgtaccgccg caatccccag
     7621 ccgtcgcgca accagctccg ccgtggaaca cagctcttcc cgcagccagc gcagggtcag
     7681 ccgtgttttc tccgcgttcc gcgcctgccg cttcgcgtgg ctgacgttgt actgctccag
     7741 tgtcatcagt aaagccccgc aaaatcatca tccgcttttg gtccgccgcc gtcggccgga
     7801 ggcaggtact ccgccagcgg gtccgttttc gccagcaccg ggaaggtttt cagcgaaatt
     7861 tcccggcgct caacccgcag cgggctgctg aacgccagcc gcgccacgcc cagcccggtc
     7921 atcaccgcga acccgtcagg caggtgttgc agggtgttca cgtcaaacag cggccgcgcc
     7981 tccttctgga tgaagctgtc gccgctgatg tgctccacgt tccccgcctc ccggctggcc
     8041 cggcggcgct ccacgtccac ccggatctcc ccggtctggc ccgccgccca ctgcgcggac
     8101 tcctggctgg ccgcgcgata gaaccagcgg atcggcgtgt tatccagtac cgtggttttc
     8161 acaaaatcag cgggcagatc ctgcccgcac tgaccgaagt cgcccagcga ctggtgcgcg
     8221 agcagcaggt tgcagtcccg gctgcgcagc gtccccagcg cgttcagcac gtacttcgac
     8281 agcagatatt taatctcatc gagcatgatg ctcgcgtgcg gccacctccg gaactcatcc
     8341 cgcgcaatga tgatttgcgc gcagcgggca aacagcatct tctgcacccg gatcaccgcc
     8401 tcatcgtcca tcgagccgat gacgtacagg cagccgccgc cgttgattat ccccgccaca
     8461 tcgataccgc tgtccgtctg caggacgctc aggtcggcca cgttctccag cttggtgatc
     8521 agccccttca cgtccttttt cagggcctcc ggcaacgcgt acgccgccgc cagcacctgc
     8581 tgaatgtttg ccccctgcgg gaactgctcc gcaatcagct tcgccgcttt ctgctcgctg
     8641 atgcggtagt ggtccgccac gtcgccggtc tcactcaggt taaacccggc aaccagcaga
     8701 ttcttcaggg cgtactggtc gagatcgcgg aacagattca gctgcgccgg tttccccggc
     8761 cgcagatcca gcagggtgaa gtccgggcag tgcgcccgga agacgtgcgg cgcccacgcg
     8821 tcccctttgg ggtcgaagta caccacagcg tccccgaaat cgcgtacgca gcgaatgagc
     8881 gcgttggtgg ccatcgcgct cttgccgctc ccgggcaggc cgaggatctg aatattggtc
     8941 ttgcgccagg tatcacgagg gaccagcacc ggctggccct gctcatcctt acccagaaac
     9001 accgcctgct gtttccgggc ctgctccgtg gaaaaatcgg ccagcgcgtt aaccggtacc
     9061 ggctccccga aatgcaggtg gcggacgtct gtcatttctc cggtccgctg gctgctgcgc
     9121 cggctctcct gattcagttt cgcgttcacc gcctcgtcag cggccgcaaa atgaccatca
     9181 agcagcagga acgccagcag ccgcgccaga aggacgccgg cgacaagccc tccggcacac
     9241 caggctatcc cgaacaggtt agccggctgc agcaggtaat aatctgcgtt cgcatagagg
     9301 tccgggcgca ggacggtttc tccccagctc tcgataaaca cggcgacaac cagccccccc
     9361 agaagggcca gaccggcagc gccggtcagc cagtacgcgt agttccagac atgtcgccgt
     9421 gccctgacca tccgcccggg tgaatccagc gaggccagca ccgaaaatgg cacagtccgg
     9481 ttattatcgc gcttatagcg atcggcatac tcccgccgcg ccttatggct cagccccaca
     9541 aaacgcattc ggccggccca gagttcagcc agaccgaaaa acaggccgat aaccagaaaa
     9601 aaaccggcca gactgtaata ctcccggtac agccagctga cccacgggta aatctgccgg
     9661 gccagctccg ggatatgcca ctgccaggca agcgaatccg tattaaacat gcaatctctc
     9721 tccttcagac gacgggttta ccggcgtgat gcgcagcccc cgctgcacaa gccgcggaca
     9781 tgccccaagg catggcggcg gcggtcacgt ccgtggtgat tgcgttcagc tttcaccgcg
     9841 gaggccagcc tctttccgcc ccatgcggaa aaggcgcagc cccggaggaa caacgagcac
     9901 gccccagcgt gtgagaggaa aaggcggcac cccagtgcgg tcttttccgg tgttccggcc
     9961 cccgtcagag ttcgccatag cgagcggctg accacccgca aaatcaggag cagctttgct
    10021 gcttcgtcag gtgcgtccag caccgtggat ttttgtgggt
//
