LOCUS       NZ_CP087616            10060 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02172 plasmid
            pDD02172-5, complete sequence.
ACCESSION   NZ_CP087616
VERSION     NZ_CP087616.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822728
            Assembly: GCF_023066625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066625.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:22:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,673
            CDSs (total)                      :: 5,549
            Genes (coding)                    :: 5,388
            CDSs (with protein)               :: 5,388
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 69 of 161
            Pseudo Genes (incomplete)         :: 96 of 161
            Pseudo Genes (internal stop)      :: 27 of 161
            Pseudo Genes (multiple problems)  :: 27 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087616.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02172"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02172-5"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(join(9956..10060,1..426))
                     /locus_tag="LMH59_RS28255"
                     /old_locus_tag="LMH59_28255"
     CDS             complement(join(9956..10060,1..426))
                     /locus_tag="LMH59_RS28255"
                     /old_locus_tag="LMH59_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(433..1164)
                     /gene="mobC"
                     /locus_tag="LMH59_RS28260"
                     /old_locus_tag="LMH59_28260"
     CDS             complement(433..1164)
                     /gene="mobC"
                     /locus_tag="LMH59_RS28260"
                     /old_locus_tag="LMH59_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(1164..3128)
                     /locus_tag="LMH59_RS28265"
                     /old_locus_tag="LMH59_28265"
     CDS             complement(1164..3128)
                     /locus_tag="LMH59_RS28265"
                     /old_locus_tag="LMH59_28265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(4612..4761)
                     /locus_tag="LMH59_RS28270"
                     /old_locus_tag="LMH59_28270"
     CDS             complement(4612..4761)
                     /locus_tag="LMH59_RS28270"
                     /old_locus_tag="LMH59_28270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(4846..5103)
                     /locus_tag="LMH59_RS28275"
                     /old_locus_tag="LMH59_28275"
     CDS             complement(4846..5103)
                     /locus_tag="LMH59_RS28275"
                     /old_locus_tag="LMH59_28275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(5113..6798)
                     /locus_tag="LMH59_RS28280"
                     /old_locus_tag="LMH59_28280"
     CDS             complement(5113..6798)
                     /locus_tag="LMH59_RS28280"
                     /old_locus_tag="LMH59_28280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            7125..7370
                     /locus_tag="LMH59_RS28285"
                     /old_locus_tag="LMH59_28285"
     CDS             7125..7370
                     /locus_tag="LMH59_RS28285"
                     /old_locus_tag="LMH59_28285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            7644..8015
                     /gene="tolA"
                     /locus_tag="LMH59_RS28290"
                     /old_locus_tag="LMH59_28290"
     CDS             7644..8015
                     /gene="tolA"
                     /locus_tag="LMH59_RS28290"
                     /old_locus_tag="LMH59_28290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            8012..8377
                     /locus_tag="LMH59_RS28295"
                     /old_locus_tag="LMH59_28295"
     CDS             8012..8377
                     /locus_tag="LMH59_RS28295"
                     /old_locus_tag="LMH59_28295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            8552..8830
                     /locus_tag="LMH59_RS28300"
                     /old_locus_tag="LMH59_28300"
     CDS             8552..8830
                     /locus_tag="LMH59_RS28300"
                     /old_locus_tag="LMH59_28300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            8818..9132
                     /locus_tag="LMH59_RS28305"
                     /old_locus_tag="LMH59_28305"
     CDS             8818..9132
                     /locus_tag="LMH59_RS28305"
                     /old_locus_tag="LMH59_28305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            9296..9724
                     /locus_tag="LMH59_RS28310"
                     /old_locus_tag="LMH59_28310"
     CDS             9296..9724
                     /locus_tag="LMH59_RS28310"
                     /old_locus_tag="LMH59_28310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            9750..9929
                     /locus_tag="LMH59_RS28315"
                     /old_locus_tag="LMH59_28315"
     CDS             9750..9929
                     /locus_tag="LMH59_RS28315"
                     /old_locus_tag="LMH59_28315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     BGC             113..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3931..4509
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(437..1164)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 tttcagcttc tcgttcaccg cgctttcccc gtgccgctcg ctgaccacct caatcatcgc
       61 gttaatctgc tccggtgagc acttcccggc atcaaccacc tgcacaccgt tctcgatggc
      121 gaaccgctcc acctttcgat agaggtaatc acacagcttc attcctgtgg gtttgtcccg
      181 ttcccattgc ggattttcac cggtgaaaaa gtacgtggct ttttcgtcgc tgttgagccg
      241 ctcgtcatac atcccgatgg atttcatcat cgccagatgc tgggcccggt cgacaaaaca
      301 gaaaacatac gccccggtct gccaggcgag caccaccatg tcttccagat gctcgctggt
      361 tggcataatg cagcgctcgc cgtctttgtc ttccacgtcc tcaataaata ccatgtacag
      421 cgccataatc cctcactccg ttgtcataaa tttaaagcac gcgaacagcc tctcccggtc
      481 cagctccacc gttttgcggg cctcgccggt ctgtggattc acgatttcat ccaccgccag
      541 aatcgcccgc tgtacccgga caagcgtcgg ccggtcaggg cagatgtacc agatttcatc
      601 gatgccgttc gcccggcggt tgaacagata cccggacacc acttcctgat accgcctgcg
      661 gctcttgacg gtgcgctcta cctcaaatgc caccgttctg ccgtccagct ccgccagcgc
      721 gtccggcact ttcatccctt tcagggccat acggtgcagg taccgccagc cggtggcccc
      781 ccggccctcc atggagaggc gcagggactg cacggccagc cggtgcggca gctgcgccgc
      841 cgacaccctt gacggctgaa acggaataac gtccgtcagc ggctcgtcct cgtcgaacga
      901 gaacgccacc ccgtgcggcg tcagccccca cacgctgacc tgacggccgt caaccgggta
      961 tttcgcccgg accagcagcc ctttcgccac cagactgtcc aggaagcggt aaaccggctg
     1021 taccgccgca atccccagcc gtcgcgcaac cagctccgcc gtggaacaca gctcttcccg
     1081 cagccagcgc agggtcagcc gtgttttctc cgcgttccgc gcctgccgct tcgcgtggct
     1141 gacgttgtac tgctccagtg tcatcagtaa agccccgcaa aatcatcatc cgcttttggt
     1201 ccgccgccgt cggccggagg caggtactcc gccagcgggt ccgttttcgc cagcaccggg
     1261 aaggttttca gcgaaatttc ccggcgctca acccgcagcg ggctgctgaa cgccagccgc
     1321 gccacgccca gcccggtcat caccgcgaac ccgtcaggca ggtgttgcag ggtgttcacg
     1381 tcaaacagcg gccgcgcctc cttctggatg aagctgtcgc cgctgatgtg ctccacgttc
     1441 cccgcctccc ggctggcccg gcggcgctcc acgtccaccc ggatctcccc ggtctggccc
     1501 gccgcccact gcgcggactc ctggctggcc gcgcgataga accagcggat cggcgtgtta
     1561 tccagtaccg tggttttcac aaaatcagcg ggcagatcct gcccgcactg accgaagtcg
     1621 cccagcgact ggtgcgcgag cagcaggttg cagtcccggc tgcgcagcgt ccccagcgcg
     1681 ttcagcacgt acttcgacag cagatattta atctcatcga gcatgatgct cgcgtgcggc
     1741 cacctccgga actcatcccg cgcaatgatg atttgcgcgc agcgggcaaa cagcatcttc
     1801 tgcacccgga tcaccgcctc atcgtccatc gagccgatga cgtacaggca gccgccgccg
     1861 ttgattatcc ccgccacatc gataccgctg tccgtctgca ggacgctcag gtcggccacg
     1921 ttctccagct tggtgatcag ccccttcacg tcctttttca gggcctccgg caacgcgtac
     1981 gccgccgcca gcacctgctg aatgtttgcc ccctgcggga actgctccgc aatcagcttc
     2041 gccgctttct gctcgctgat gcggtagtgg tccgccacgt cgccggtctc actcaggtta
     2101 aacccggcaa ccagcagatt cttcagggcg tactggtcga gatcgcggaa cagattcagc
     2161 tgcgccggtt tccccggccg cagatccagc agggtgaagt ccgggcagtg cgcccggaag
     2221 acgtgcggcg cccacgcgtc ccctttgggg tcgaagtaca ccacagcgtc cccgaaatcg
     2281 cgtacgcagc gaatgagcgc gttggtggcc atcgcgctct tgccgctccc gggcaggccg
     2341 aggatctgaa tattggtctt gcgccaggta tcacgaggga ccagcaccgg ctggccctgc
     2401 tcatccttac ccagaaacac cgcctgctgt ttccgggcct gctccgtgga aaaatcggcc
     2461 agcgcgttaa ccggtaccgg ctccccgaaa tgcaggtggc ggacgtctgt catttctccg
     2521 gtccgctggc tgctgcgccg gctctcctga ttcagtttcg cgttcaccgc ctcgtcagcg
     2581 gccgcaaaat gaccatcaag cagcaggaac gccagcagcc gcgccagaag gacgccggcg
     2641 acaagccctc cggcacacca ggctatcccg aacaggttag ccggctgcag caggtaataa
     2701 tctgcgttcg catagaggtc cgggcgcagg acggtttctc cccagctctc gataaacacg
     2761 gcgacaacca gcccccccag aagggccaga ccggcagcgc cggtcagcca gtacgcgtag
     2821 ttccagacat gtcgccgtgc cctgaccatc cgcccgggtg aatccagcga ggccagcacc
     2881 gaaaatggca cagtccggtt attatcgcgc ttatagcgat cggcatactc ccgccgcgcc
     2941 ttatggctca gccccacaaa acgcattcgg ccggcccaga gttcagccag accgaaaaac
     3001 aggccgataa ccagaaaaaa accggccaga ctgtaatact cccggtacag ccagctgacc
     3061 cacgggtaaa tctgccgggc cagctccggg atatgccact gccaggcaag cgaatccgta
     3121 ttaaacatgc aatctctctc cttcagacga cgggtttacc ggcgtgatgc gcagcccccg
     3181 ctgcacaagc cgcggacatg ccccaaggca tggcggcggc ggtcacgtcc gtggtgattg
     3241 cgttcagctt tcaccgcgga ggccagcctc tttccgcccc atgcggaaaa ggcgcagccc
     3301 cggaggaaca acgagcacgc cccagcgtgt gagaggaaaa ggcggcaccc cagtgcggtc
     3361 ttttccggtg ttccggcccc cgtcagagtt cgccatagcg agcggctgac cacccgcaaa
     3421 atcaggagca gctttgctgc ttcgtcaggt gcgtccagca ccgtggattt ttgtgggtgg
     3481 tcaggcgtga gcctgacggg ggccgggtga atttctggcg tggccggcgt ccagccggac
     3541 agagccagaa agaggggaaa agaccgtgcg tcggtggcgt ccagccagcg taagcaaggt
     3601 cttttcccct gaagtcagcc acgccccagc ggggatgact tgcatttctc cgcaaggaga
     3661 aaaaagcaac agagataggt taacgtaaaa tgttcagcga aaaaccgaca tggttcacct
     3721 atcctgataa ttgatcgtca gggaatagaa agacttaatc aggggacaat atcccacatc
     3781 agcgcgtccg gtgaccgtct tgcaggtgcc ggggaaattg cgcctcctcg ctcactcggt
     3841 cgctacgctc cgggcgtgag actgcggcgg gcgctgcgga cacatacaaa gttacccaca
     3901 gattccgtgg ataagcaggg gactaacatg tgaggcaaaa cggcagggcc gcgccggtgg
     3961 cgtttttcca taggctccgc cctcctgcca gagttcacat aaacagacgc tttttcggtg
     4021 catctgtggg agccgtgagg ctcaaccatg aatctgacag tacgggcgaa acccgacagg
     4081 acttaaagat ccccaccgtt tccggtaggt cgctccctct tgcgctctcc tgttccgacc
     4141 ctgccgttta ccggatacct gtccgccgtt ctcccttgcg ggaagtgtgg cgctttctca
     4201 tagctcacac actggtatct cggctcggtg taggtcgttc gctccaagct gggctgtaag
     4261 caagaactcc ccgttcagcc cgactgctgc gccttatccg gtaactgttc acttgagtcc
     4321 aacccgggaa agcacgcaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
     4381 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
     4441 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc
     4501 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc
     4561 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
     4621 cgcgatcccc gtcagttcag aggaggacga tggtgccacc gttccgccct gaacatcccg
     4681 gatataattt gcctgacatg cggccagaag aaagccagca acaatcaata taaaaagaaa
     4741 aatagtcttt acctttttca tatactttcc ctgtatttaa agactccggc attcatccct
     4801 gaacaccgga agtcagtatt tcaaatcatc cttgataatt ttttgttacc agttaccgtc
     4861 ccgataatca aaagctatat aacaattatc cacatcaggt tttatttgat ttttaaaaaa
     4921 tggctgtagt aaaggaaccc attctttctt caaatcaaac tcaccattat taatattatc
     4981 ctttaaaggc attcccaagc tttcaatgac agaaccatcg tcaccaaagt ctttagaata
     5041 ctcttcccct ttaaactctt cagttttctt atcaaaccat gtcaaacgta atttaagccc
     5101 catatttacc tctcaaagat attttttaat gttccgtttt ggatctggcc ctttaacctg
     5161 cttacccgtt ttgggatcaa atgcgcctag gtgttcgcca tcactggcac gatacccttc
     5221 aagctcaccg tgctgggaat cccattcata aattttacgt tttttatcgc cataccatcg
     5281 ggcccgctta ccaccaccac cttgtttagg tgttttagga tcacctcgct ttaactctcc
     5341 aagtcccttt atgtcttctg ttttaggtac tggatgataa tcatggccgt agtctttagt
     5401 ccccttgcga ggcttgttct tttcctcatt taacttattt tctgcatcct tcgctttttt
     5461 ctccttttgt ttccggctct ccatcgcccc ggtaagtgca gccttatctc tggataagga
     5521 ttcattcatt gcgtttaaag atgcctgagc cgcttttaat ttctcattct gattctgaat
     5581 atccttatca agatagcgaa cctgatcctg ccacccggag ccatattcat gaggcgggtt
     5641 ataagtcaca aaatcatctt tatttgcctc tgcctctttt acctttttat cggcttcctg
     5701 aacagcagcc cttgcaccgg ggagcgcctt ctctgtcgat gcaatgcggc tgtttaaggt
     5761 cgcaatattt ttatcttcgg catccagctc agctttcgct ttatcatagt ctcttttcag
     5821 cccctcttcc ggatgagcgg cgtcccatgc ctgctggcga cgcttttctt cctcctgacg
     5881 ctgttttacc tgtgccgggg taagaacatc tgtcaccgac acataaaccg gcttctgtcc
     5941 ggtctctttc gggaaacgaa taacggcctc acgggagtta ccaccggctg taaaaccagc
     6001 cggacgtgaa tcaccttttt cagcaacaat gccttttggc ggggctttcg ctgtcggaac
     6061 acctttcggt acgctcacct gcagagacgg gagacccgga atcgacacac tgaatacccc
     6121 cggacgtttt gtcggtttcg catccaccac agggacactc attggccgac ctgcgacaac
     6181 cgcaatatgc tgccgctcat ccttcaccac atccacaacc cgttgtctga cacgaaccgt
     6241 cgcctggtcc agtggtaaag tacttgccgg agtctccgtc accgcatcgg ccggtaatga
     6301 cgtcataatt tttgacatca ttttcggatc atcttttgca atctcagaag gcagcacacc
     6361 gtagatcgca atcccccaca gaccaaactt aaacggcccc ttcagggcag ccagcacatc
     6421 agcaacggcg gccgacaacg catcgccgga aactgataaa gccagtcctt cagcccccgg
     6481 agtagccagg gcaggcagac cgaaggccat cgcagtggcc gaagactgtc cgccaccaga
     6541 gctaccgcta tgattgccgc tgttaccacc attaccaccg ggtttactcc cgccgttacc
     6601 aaactccccc ggattatagt tatgaatatc tccattcggc gtatgcgtcg taccccagcc
     6661 cggcccgctg tttggatccg taccaccacc tgatgaaggg ccacctttac cagacgtacc
     6721 gttcacattc ccactggcct gaccaccatt atgtcccaga cctgaattac ccggacctcg
     6781 accgtctcca ccactcatac cgttttcctc tttgacattt aaaacaataa attaaagcca
     6841 acatactgta catatacaca gtataattat taccttactc ctcccgtttt caggtgtcaa
     6901 tatatgagat tagttctcat cttcaggcac aaaaaagcgc ccggagccac ctgtaagcag
     6961 atcgcgtctt ttcccgaaaa tttctcatgg caacaaattt tattatttaa gacattcatc
     7021 acacttacat ttcagatggt atagacacat ctgaaagcta actggcatga ggagaggacc
     7081 tgcggttaaa ctgttcggga aacctgagac gcccggaggc cactatgacg cacctgaaac
     7141 tgactcccgc tgaagaaaag cgcctggcgg acgcctttgt cgctcaggca ctggccatgg
     7201 gcctgaaagg gaacaaaacg ctgccaaagg cggtaaaaca acaggtgcgg gaaagggtgc
     7261 ttcgtgccat tcaggacgaa cgtgaagcca ggcgggcgca ggaaatgcgt cgggagaaat
     7321 acgaggctac ggaaaccacc ttcacctggc agccacagcg ccgccggtaa ggggtattta
     7381 ccataacgtc gcattgcccg caccgctcag acggtctgag ggcgtttccg gcggctacgg
     7441 gataaaacag gcaacaaacc acccggaaaa ccgccggaaa cgcgcccggt aaattgtaac
     7501 ccgctgcatg cctatgcacg caactgcatg gcgggccgtt tggtgtaaaa acgatacgat
     7561 aagccgtgtt tatgtaaatg aacgtggccg taaagagtga caaatataca tgtaacattt
     7621 tttactgaca ggtcatcaac ccgatgcgtt taataccctt tatcctgctg gcattgtgca
     7681 gccccttact cctgacggcc tgtaagcatt ccaccaccca gccgccgaaa atttatactc
     7741 tggataagtc atctaaaatc agtagctacg ctgcggccat caagaccgct gttgaaatgc
     7801 agatgtttga accagaggcc tatgcgggaa aaatgtgcac cgtgcggata tccatacaac
     7861 gtgatggcac tctgaacagc gccattgctg aaggaggaga tccgaaactc tgtaaagcag
     7921 caatatcagc cgttacccac gccaaaattc cgccggcacc ggatgaaaat acctggcaaa
     7981 tattcaaaaa cgcccctctg gatttcagac catgaaagct tttactctga cagtgctggc
     8041 ggccatgttc ctcactccgg tacatgcgat accactaaaa cccgtaacca cactgcagcc
     8101 aacctacccg gtacaggccg cagcaaaacg catcgaaggt caggtgaccg ccacgtttga
     8161 tattctggac gatggacgag tggacaacgt gtcgcttacc ggctgtcacc ttttttttcg
     8221 ggaaacgctg acggcaatga ggcactggct atatcccgca gggcacccgg caatgcgaca
     8281 ggttataaat tttgaattca gtctgaaaaa tgacggctta caggatccgc gaccagtcac
     8341 cacccaaaag ctcaatgatg atggttgccg ccagtaacag gtcctttaac ccgcaggctg
     8401 caatagtcct ggatgatgag tgagcctgta gcagccctga ttagtggaag cgaagctgaa
     8461 aaatgggcag caaaagccgt atttctggca aatagcgccg ccacggatta aagtagtcat
     8521 gtgtaataca tatgactaca aaggggcttt tatgtcatca gcaacatcca tcaggctcga
     8581 tgaagaactt aaggaccggc tgaaaacgct ggccgatgac cggcaccgct cagcgcacgc
     8641 gctaatgctg gaggcaatca ccgaatacat cgaccgggaa gagaagcgca gccagtacct
     8701 gcgggacgga caggccgcat ggcagcacta tcaggaaacg ggtctgcacc ttactgcgga
     8761 agaagcggaa gcctggataa gtacctgggg aactgagaac gagcaggatg ctccgccatg
     8821 ccacaggtaa cgatttccgc gctggcacag cgcgatttac aacgccttca ggactttctc
     8881 aaaaccaaaa atcggctggc agccagaaaa ggaggtgagg tgatcgtccg ggctatccag
     8941 caactgaaaa cattgccaga cattggccgc ccggtgccgt ttctgccgct ggaatataag
     9001 gaactggtga tcgggtttgg cgacagtggc tatgtgatgc tctaccgcca cgaccgggaa
     9061 atggaccaga ttgtgatcgt caccgtcagg catcagaaag aatccgggta tccgggggca
     9121 gacagcctct aataaccgat gacgggggcg caattcattg cgtggccgtc gccggtttga
     9181 caagcgcagc gcgtcagaaa accacatcct gaacggaaaa acgggtaaaa ttcagcgata
     9241 catttaccgt gagaaattac acacctttca cacccgataa aatcaggggt atacagtggc
     9301 gaaaacattg ctcaaccttt cacaggcagc acaagccgcg ggcattacca gaagaacgct
     9361 atacaatcat gtaaaacagg gaaaggttac cgtgtcacgt gacgggaaaa acaaccctgt
     9421 cgtggatgtg tcagaactga tccgtgtata cggaaatgtg aatatccctg agaaacaaat
     9481 tcccggcatt tcacaccgtg aaaatacaca aaaaaatttc ccacaggaac agcttctggc
     9541 aatgcaaaag gaactggccg atctccggca ggctgtcacc ctgatgctgg aagataaaac
     9601 atcccgggaa gaagaacgcc gccagcacga tgatgagcgc cggaaactcc aggccgaagt
     9661 ggacagattg acaacagaac tgacacagaa gaaaaaacgc ttctggtccg gctggtttag
     9721 ctgagaactt accgaaggga atggagaaca tgacgaagca ggaaaaaact gccctcaata
     9781 tggctaaatt cattaaggat cagtcgctgc tgttgctgga aaagctgaac gagctggaca
     9841 tggatacccc ggccgacatg tgcgaacggc ttcacgagga tgcggaaaca ctctggcagg
     9901 tgatgcggga taagctcggg gaagaataac agcaccggcc atgccggtgc tggcgttatt
     9961 ttttgccgaa cggccaccac cggcgggcgg gtttgtcctc cggcagttca ggcaccggca
    10021 cgttaaaata gcggctgtta tattcccgca tcagctccgc
//
