LOCUS       NZ_CP087615            11057 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD02172 plasmid
            pDD02172-4, complete sequence.
ACCESSION   NZ_CP087615
VERSION     NZ_CP087615.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822728
            Assembly: GCF_023066625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066625.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:22:24
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,673
            CDSs (total)                      :: 5,549
            Genes (coding)                    :: 5,388
            CDSs (with protein)               :: 5,388
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 69 of 161
            Pseudo Genes (incomplete)         :: 96 of 161
            Pseudo Genes (internal stop)      :: 27 of 161
            Pseudo Genes (multiple problems)  :: 27 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087615.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD02172"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD02172-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            join(<10628..11057,1..538)
                     /locus_tag="LMH59_RS28200"
                     /old_locus_tag="LMH59_28200"
                     /pseudo=""
     CDS             join(<10628..11057,1..538)
                     /locus_tag="LMH59_RS28200"
                     /old_locus_tag="LMH59_28200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341074.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(947..1825)
                     /locus_tag="LMH59_RS28205"
                     /old_locus_tag="LMH59_28205"
     CDS             complement(947..1825)
                     /locus_tag="LMH59_RS28205"
                     /old_locus_tag="LMH59_28205"
                     /inference="COORDINATES: protein motif:HMM:NF025663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp fold amidoligase family protein"
                     /protein_id="WP_157280122.1"
                     /translation="MIKFFFGKAYRLLLENLSTTNRVKLRYFRVYKKLPNIKKPKTFNE
                     KVISRILGEQKDIYSTLADKYLVRRFVSERVGEEYLIPLHLATSNPSDLLKIDDWSGLV
                     IKPNHAAGMIYISDDNPSKEEKDNVVTTAKTWLKTDFSSFCDEMHYSKIKPMLLVERKI
                     TKRGEIPRDYKFHCFKQKNGDINFVLQLVDGRFGQESRSYFLNSFENMVWSHGAGNHLL
                     SNNEVAVLNEVILLNKRLILNDFNYVRIDWYILGDRIYFGEITFTPGAGIGNEFGPVLE
                     KQMAEWWEDEY"
                     /name="ATP-grasp fold amidoligase family protein"
     gene            complement(2106..3329)
                     /locus_tag="LMH59_RS28210"
                     /old_locus_tag="LMH59_28210"
     CDS             complement(2106..3329)
                     /locus_tag="LMH59_RS28210"
                     /old_locus_tag="LMH59_28210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013512451.1"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_227525306.1"
                     /translation="MMAEKLVSIIGLVFINSYMAKYIGPTDFGKITFATTIFTFVQTMA
                     WFGGQNILFKRMSEKRKSGIALALHTQKQRRVIFALSSLIALFYLFFFTDTLTFVFGVA
                     NCIATYYIVMDVFAVYNNTQLISRINALTNGAGLCIAFIVRFLISYLELPVYTFSIPII
                     LIPAIPYVIRYIYFHNTIEDTAVLKSKTKKYNKYLFSAGGSLLLSTLSIVIYTQVSNIF
                     LAKITSYSDLGIYNVALTLGSAYSFIPVALITSFFSKIYQEKDFEKMSRLLTLINRIVI
                     VITIFILFGFYFLGPYIISMLYGSAYKLAVELVPIIILATMFSSLGTICYRFIIKYSGY
                     KYLSYKMTLTSLLSIPFSYVMINRYDVFGAAYCFLVVEVLSCTLLNYFFRNGMILRIHL
                     NVIGLMRR"
                     /name="oligosaccharide flippase family protein"
     gene            4137..4834
                     /locus_tag="LMH59_RS28215"
                     /old_locus_tag="LMH59_28215"
     CDS             join(4137..4386,4386..4834)
                     /locus_tag="LMH59_RS28215"
                     /old_locus_tag="LMH59_28215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_225739689.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTVLRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLATLERLQSLLSAFEVVVW
                     MTDGWPLYESRLKGKLHVNSKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVEQHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            5299..5409
                     /locus_tag="LMH59_RS28220"
                     /old_locus_tag="LMH59_28220"
     CDS             5299..5409
                     /locus_tag="LMH59_RS28220"
                     /old_locus_tag="LMH59_28220"
                     /inference="COORDINATES: protein motif:HMM:NF033853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein"
                     /protein_id="WP_142740574.1"
                     /translation="MDWILLAIAIALLVVAIYSGISYFREKKSISRVFRR"
                     /name="small membrane protein"
     gene            6458..6679
                     /locus_tag="LMH59_RS28225"
                     /old_locus_tag="LMH59_28225"
     CDS             6458..6679
                     /locus_tag="LMH59_RS28225"
                     /old_locus_tag="LMH59_28225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            6672..7559
                     /locus_tag="LMH59_RS28230"
                     /old_locus_tag="LMH59_28230"
     CDS             6672..7559
                     /locus_tag="LMH59_RS28230"
                     /old_locus_tag="LMH59_28230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="WP_114450885.1"
                     /translation="MAKFASAARQATGVIKILQGDILRSVGTVRNYEQGLTRVAEWMKD
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="site-specific integrase"
     gene            7628..8254
                     /locus_tag="LMH59_RS28235"
                     /old_locus_tag="LMH59_28235"
     CDS             7628..8254
                     /locus_tag="LMH59_RS28235"
                     /old_locus_tag="LMH59_28235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227543526.1"
                     /translation="MGVYYPDASNPQLFTGWKAVTELGPAILATAIAATAFSLWLFILF
                     FRSSESRRIQSETDRYKQALREAEEAHTARQNALIRDHNMKTETLHSHYCDAANVLREE
                     YAKREKRWRQALQNAREEARQELEEQTEALNQRASGLLVQEQTLKARMEEVRKYVAGER
                     HNAMRARLEAEEAEKRRQNASATAERRRRKLEKLQATDGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(8261..8665)
                     /locus_tag="LMH59_RS28240"
                     /old_locus_tag="LMH59_28240"
     CDS             complement(8261..8665)
                     /locus_tag="LMH59_RS28240"
                     /old_locus_tag="LMH59_28240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_114450884.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLDTSE
                     LLRVFGAFAPVSVEQEEEQVSGQRDTVTSDRNEQVIYILKQEIEHLNTLLASRDSHIDS
                     LKQAMLLLENREEKRQNTTRPRAWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(8665..8856)
                     /locus_tag="LMH59_RS28245"
                     /old_locus_tag="LMH59_28245"
     CDS             complement(8665..8856)
                     /locus_tag="LMH59_RS28245"
                     /old_locus_tag="LMH59_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            9276..9389
                     /locus_tag="LMH59_RS28445"
     CDS             9276..9389
                     /locus_tag="LMH59_RS28445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_073511950.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPERHANAPLAAATGN"
                     /name="replication initiation protein"
     gene            9840..>10462
                     /locus_tag="LMH59_RS28250"
                     /old_locus_tag="LMH59_28250"
                     /pseudo=""
     CDS             9840..>10462
                     /locus_tag="LMH59_RS28250"
                     /old_locus_tag="LMH59_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073174.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     Typing          8905..9389
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="90.984"
                     /coverage="92.0"
                     /evalue="0.0"
ORIGIN
        1 acccaacagg ctccagcgac agttcaatcc ggatgctcct gatgagcgtt gggtaacgga
       61 cataacctac atcaggaccc acgaaggctg gctgtatctt gccgtggttg ttgatctgtt
      121 ctcacgcaaa attatcggct ggtccatgca atcccggatg acaaaggaca ttgtcctgaa
      181 cgcactgctg atggctgtat ggcggcgtaa tcccgaaaaa caggtgctgg ttcattcgga
      241 tcagggcagt cagtacacaa gccatgagtg gcagtcgttc ctgaaatcac acggcctgga
      301 gggtagcatg agccgtcgcg gtaactgcca tgataatgcg gttgcagaaa gttttttcca
      361 gttgttgaaa cgtgaacgga taaagaaaaa gatctacgga acgcgagaag aagcccgcag
      421 tgatattttt gattacatcg aaatgtttta taacagtaag cgtcggcatg gttctagcga
      481 acagatgtca ccgacagaat atgaaaaaca gtattatcaa cggctcggaa gtgtctagat
      541 tatctgtggc gattcagtcc aacccttacg gacacgatac atcgccactg gtaacgggtg
      601 tacaagattt actgactcag agttcttgaa gttaaagcct tactgcggct aaactgtaag
      661 gacaaattag gttgcagcgg tcttgtacac cctgttacca cggttcagaa gttccccaac
      721 ttactgaccc atcgaaaaaa ccaccacccc aggtggtttt ttcgtttctg ctacttagat
      781 tacgatgcag tgactccaat ctcaaggaaa aacgatcagg tttcgaaagt aggtaagaac
      841 atcatcaaaa gatcaccttg cttgtaaaaa gtaccttcag ccaaaggcga taatcaagaa
      901 gaaatagcgc atacaacttg caaataccac gagcgtagtg catgaattaa tattcatcct
      961 cccaccactc agccatttgt ttttccaaga caggtccaaa ttcatttcca attccagccc
     1021 ctggagtaaa tgtaatctca ccaaaatata ttcggtcacc taaaatatac caatcaatac
     1081 gaacgtaatt aaaatcatta aggataagtc ttttatttaa taaaatcact tcatttaaaa
     1141 cagcaacctc attattactt aataaatgat tgccagcgcc atgactccaa accatatttt
     1201 caaaagaatt taaaaaatag cttcgtgact cctgcccaaa gcgaccgtcc actaactgca
     1261 acacaaaatt aatatcacca tttttttgtt tgaaacaatg aaacttgtaa tcacgaggga
     1321 tttcaccacg ttttgttatt ttcctctcca ctaaaagcat tggctttatc ttggagtaat
     1381 gcatttcatc gcaaaaagag gaaaagtcag ttttcaacca ggttttcgca gtggtaacaa
     1441 cattgtcttt ctcctcttta gagggattgt catcggatat ataaatcatg cctgccgcat
     1501 gatttggttt gataaccaat ccagaccaat catctatttt taataagtca ctagggttgc
     1561 tagtagccaa atggagagga ataagatact cctctccaac ccgctcggat acgaaacgcc
     1621 gcacaagata tttatcagcc aatgtggagt atatatcctt ttgttctcct agtattctac
     1681 taataacctt ttcattaaag gttttaggtt tttttatatt tggaagcttt ttatacactc
     1741 taaaatatct tagtttaact ctattggttg tagacaaatt ctccaaaagc aacctgtaag
     1801 ccttaccaaa aaaaaactta atcacttcaa ttctctcttt ttaatctgtt aaatcagaaa
     1861 gcatgtatac tcgagacact ccaagtttca tgcgccttgg cttcttgatg cactgcagat
     1921 aagggttcca cggaactcag tctacacact caatcttatt aaacatacag ataaataata
     1981 aaaaatcata agtaacaata aaattcaaaa actcaccccc aacacaccag aactaatcat
     2041 catattttaa atctcataag aaaatcaata aaacacaatc cataaataaa cctgcccccc
     2101 caccatcaac gcctcatcaa cccaataaca tttaaatgaa tccttagaat cattccattc
     2161 ctaaagaaat aatttaataa tgtgcatgac agaacttcga ccacaagaaa acaatacgca
     2221 gcaccgaaaa catcataacg gttaatcata acatatgaga atgggatgct gagcaagcta
     2281 gttaacgtca ttttatatga aaggtatttg tagccagagt attttattat aaatctataa
     2341 caaatagttc ccaaggatga aaacattgtc gctagaataa tgattggcac caactcaaca
     2401 gccagtttat aggccgaacc atagagcatg ctgataatgt acggcccaag aaaataaaaa
     2461 ccaaataata taaaaattgt tattacaatc acaattctat ttattaaagt tagaagccta
     2521 gacatttttt caaaatcttt ctcttgatag attttagaaa aaaaactagt aattaacgca
     2581 actggaataa atgaatacgc actgccaagc gtcagcgcca cattataaat acccaaatca
     2641 gaatatgagg ttattttagc caaaaaaata tttgatacct gtgtgtaaat cacaatggac
     2701 agggttgata gtaacaacga ccctcccgct gaaaacaaat acttattata tttcttcgtt
     2761 ttcgatttta ataccgcagt gtcttcaatg gtattgtgaa agtatatata tctaataaca
     2821 taaggtattg cagggattaa tataatagga atgctaaatg tatatactgg caactccaag
     2881 tatgaaatta aaaagcggac tataaaagca atacacaacc ctgcaccatt tgttaatgca
     2941 ttaattctag atattaactg cgtattatta tatacagcga aaacatccat cactatataa
     3001 taagtggcaa tgcagttagc caccccaaaa acaaatgtca gggtatctgt aaaaaaaaac
     3061 aaatagaaca aggcaatcaa tgaagagagt gcaaaaataa ccctgcgctg cttttgagta
     3121 tgtaatgcaa gagcaatacc tgattttctt ttctcactca tccttttaaa aagtatgttt
     3181 tgcccaccaa accaagccat ggtctgtaca aacgtaaata ttgtcgtagc aaaagtaatt
     3241 ttgccaaaat cagtaggtcc tatatatttt gccatatagg agtttataaa cacaagaccg
     3301 atgatgctga ctaatttttc agccatcatc caaaaggcat tagaatacat tactcactcg
     3361 caaaaaatta atcccataga aattttggta atgctgtcaa cttgttgata actattttaa
     3421 tctgtagtca ttcaatcctt gtaacatcag gataatccta gtgtcagtga aaatacgaaa
     3481 tcaacaagtt ggtatcatta ccagcgtcgg catggttcca gcgatcagat gtcaccgaca
     3541 gaatatgaaa accagtatta tcaacggctc ggaagtgtct agattatccg tggcgattca
     3601 aaagctgatt tttgtcgtgg tctaactact gcaaagaatc cacctaaaag ctctcttttg
     3661 acatatcttc tgcatcttat aaattgaata aaacctataa aaaacaacca tttaggcacg
     3721 ctaccgccct aggctcttca gctacctatc cactgcacta acgctaacga ctatctttaa
     3781 catttgggtt gaggtcaaat cattaagtaa cttaacaata aacacgataa cttcaggaag
     3841 gaccgatttc acccttgctt atgatggcgt aacgttttct tatcatggaa ctttcacgag
     3901 acttgccatt ttctgcaaat tccgattcct ttacacttat cagtatactg actcagtgga
     3961 ctaacatcgc cgttgatgca attacagaat taacaaaaaa gtggtggcca ccgctgcgaa
     4021 tgtacatgac ctgaatcagg ttgaaaacct gatcacagag cctatggaac acataattta
     4081 aggtaatgct gccaacttac tgatttagtg tatgatggtg attttaaggt gcttgcgtgg
     4141 cttccatttc catcagatgt ccttcctgct ccgctactga aggcgtggtg cgtaacggca
     4201 aaagcactgc cggacatcag cgctatctct gctctcattg ccgtaaaaca tggcaactac
     4261 agttcactta caccgcctct cagcccggta cgcaccagaa aatcattgat atggccatga
     4321 atggcgtcgg atgtcgcgcc agtgcacgca ttatgggcgt tggcctcaac acggttttac
     4381 gtcacttaaa aaactcaggc cgcagtcggt aacctcgcgc atacaaccgg gcagtgatgt
     4441 gattgtctgc gctgaaatgg acgaacagtg gggctacgtc ggtgctaaat cacgtcagcg
     4501 ctggctgttt tacgcgtatg acaggatacg gaggacggtt gtggcgcacg tcttcggtga
     4561 acgcactctg gccacactgg agcgtcttca gagcctgctg tcggcctttg aggtcgtggt
     4621 atggatgacg gatggctggc cgctgtatga atcacgcctg aagggaaagc tgcacgttaa
     4681 cagcaagcgt tacactcagc gcattgagcg acataacctg aatctgagac aacatctggc
     4741 aaggctggga cggaagtcac tgtcgttctc aaaatcggtg gagcagcatg acaaggtcat
     4801 cgggcattat ctgaacataa aacactatca gtaagttgga gtcattaccc acgtctcgat
     4861 atcaccaccc acagccttta cgcctggata aagaagtacg gtccggatac cggatcccgc
     4921 gggcacgtaa aggcgaggcc agtatcgtgt cgcccaacag gctccagcga cagttcaatc
     4981 cggatgctcc ggatgagcgt tgggtaacgc cccaataggc agtgtaagtt aactgtcgct
     5041 gccgcgtttt acggcagtgg ttgcagacaa gcaggggggg gggaacatgc ccataactca
     5101 tgcacggttt ttttgttttt cctgtatgta caaaaaacat aactaggaat aatcttagca
     5161 gccttaacaa acacatacaa aaacagtggc tatgtttcat ctcaccacaa taagtaacga
     5221 cagtatcatg ctaaaaaaag atgatattat ctttatgaaa tcataacagg ctgtttttat
     5281 taaattaatt aggcatttat ggactggatt ttacttgcta tcgccattgc tctacttgtt
     5341 gtagctatct actcaggaat ctcatacttc agagaaaaaa aatcaatcag tcgtgtattt
     5401 aggcgatagc cccctattta ccataatccc ttcttacgcg caccgcgaaa acggcctcag
     5461 ggcgtttctg gcaggaaacc ggtaaaacag gcaacaaacc acccgaaaac ccgccagaaa
     5521 cgcacctgat ttattttacg catatgcatg gctatgcaca ccaccgcata gccggtgatt
     5581 tcggtcgaga aaagggttct ggacgttaaa agcataaaag aacgggccat ccaggcccgt
     5641 caaatgagga caacactgta aaaggaccgt cgcacctggt gcggtgccgg cgataagaaa
     5701 aaggtagctc atatcagccc ctcaccggag aaaaatattt cctggccacc tcaaccgcca
     5761 cacgctgtgt tcccgttaac tcgcgcagat aacgcaggaa catcctgttt tcctctgcat
     5821 gcgcctcctc actgccctgt gcgtggtatt ccactatcgc gttcaccacg gactcaccga
     5881 cctgcatcgt ctgacctcat ctgcactttc atcacaccgt ttcagatccc gcctcctcgc
     5941 catgatgtga aacggtgacc gggcaggcaa attttttgtc tgtccggtca ccgttggcgg
     6001 ggtccggggc ggtcagtccc ggtgtttttt attccgccaa atcagcacct gcgacacccg
     6061 ctatgtacga cgcataaatg cgcctgtaca tagcgggtgt cgctattgag cggagtcaga
     6121 cgcaacattt aaaaaacaac attcacacgc acgaaaaacc tggctagtat ttagtcaaac
     6181 catgagcccg cgcactctga cgcgcacctg cctaccggca gcaggtaaaa cagccgggtc
     6241 cctccgggga tactgtaccg ggcagcccaa tccgagaaca ctgagacaac agcccaaatc
     6301 tgtcagaccc acaaccgggt caagtcattc tctgactaaa gagacgttga gccgcgagca
     6361 gtccgggctt aaccacgtta cggtttaaac gagggcgatc ggcgcaggcc gcagtggatt
     6421 tccactggcg ttcttacaac tcaacaccgg agttcctgtg gaatttgata ccgccgccga
     6481 actggccgcc ctgcaggcgc agacccgccg tatccgccag gtccgctacc gtccctcccg
     6541 ccttgaccgc tataccggcg aactgctgag cctgtaccag gccggggcca gtgcagcgga
     6601 gctgcaacgc tggctccggg cccggagaat caaagtggtg ttgtccacgg ttacccgctg
     6661 gctggaaaaa aatggctaaa ttcgccagcg ctgcccgcca ggcgacgggc gtgataaaaa
     6721 tcctccaggg cgatattctt cgctctgtcg gtacggtccg gaattacgag cagggactga
     6781 cccgcgtcgc agagtggatg aaagataacc gcatcaacca cggtgattta cgcacactga
     6841 caccggcgca ggccatcacc tacctggagc agcgcgggga gcaggtcggc cagaaaacgc
     6901 tcgatatgga acgccaggcc atccaggcca tgatgatcca cgtgacgcag accctccctg
     6961 ccggcgaaca cctgccggtg gtgaaatcgg agcaggcaca gatcctgagc agccgggcct
     7021 acagcccgga ccaggtggcc ctgattgccg ctgcacagca gcccgccaac gctctggcca
     7081 ccgcgattgc tctggccgcc ggattacgcg cccatgaact gctgaccctc gcgccccgcc
     7141 aggagcgagc agcggatccc cgtccggcag cggacagtaa atttgccggt cgggagggtg
     7201 tgatttacac cgttcagggc aaaggcggcc tggtcaggga agttctgctg ccgtcacggc
     7261 tggctgagcg tctggagcat gtcaggctgg cgagcccggt ccgggtgacc gatcgcggcg
     7321 tgttttacca gagtcagtac gccattggcg gaggacagcg ctggtcgaac gccttcagtg
     7381 ccgcctccat gcgtaccctg ggctggtcac ggggggcaca cggcgtgcgt cacagctacg
     7441 cccagcaacg gatgcaggag ctgcaaaaac tcggtctggt tcgcgatatg gccctgcgca
     7501 ccgtcagcca ggaaatgggc cattttcgtc cggaaatcac ggagacatac ctgcgatgac
     7561 aagaacgttt attttattgt gtaccggtgt tttcgccatc gcgtacgccc tgtatgagct
     7621 gaaacatatg ggcgtttact atccggatgc cagcaatccg cagctgttca ctggctggaa
     7681 ggcggtcacc gaactcggtc ccgctatcct ggctaccgcg atcgcagcca cagcgttctc
     7741 gctgtggctg ttcattttgt ttttcagaag ctcagagtcc cggcggatac agagcgagac
     7801 agacaggtat aagcaggctc tgagggaggc agaagaggcg cataccgcca gacaaaatgc
     7861 ccttatcagg gatcacaaca tgaagacaga aaccctgcac agtcactatt gtgatgcggc
     7921 gaatgtactg cgggaagagt atgcgaaaag agaaaagcgc tggcgtcagg cgttgcagaa
     7981 tgcccgggaa gaagccaggc aggagctgga ggaacagacg gaggcactga atcagcgcgc
     8041 gtccggcctg ctggtacagg agcagacact gaaagccaga atggaggaag tcagaaagta
     8101 tgttgccgga gagcggcata acgccatgcg ggcccgtctg gaggcagagg aagcagagaa
     8161 aaggcgacaa aatgccagcg caacagcgga acggcgcaga cggaaactgg aaaaactcca
     8221 ggccactgac ggaacagcag ggcaaaaaca ctgaggcatt tcaggactgc cagaaacgcc
     8281 accaggcacg gggccgggtc gtgttctgcc gcttctcttc ccggttctcc agcaacaaca
     8341 tcgcctgttt cagactgtcg atatgtgaat ctctggaggc aaggagggtg ttcaggtgtt
     8401 caatttcctg tttcagaatg taaataacct gttcattacg gtcagaagtg actgtgtcac
     8461 gctgtccact gacctgttct tcctcctgtt caacggatac gggcgcaaag gcaccaaaca
     8521 cccgcaaaag ctctgaagta tcgagcattt tcgcattccg ttcaccccga caggtggaca
     8581 gttcaccggc tttaattaac ctgtgcaggg ttgtcctgct cttacccgtc aggcgtgcag
     8641 cctcactgat cgacactttc gccatcagtc accctcctgt agtgcatcca gtctgaggga
     8701 cagtgtacgg aaaagatgct ccgcatcatc gtggagtttc tcacaaaggt cagcctccgc
     8761 atcgagatca agctcgttaa gcttctccag caggagcagg gactgattct ggatgaattt
     8821 agccatgttc agggcagttt tctgttgttt cgtcataagg ggcctcaggg cgtcagtgct
     8881 cagtgtaaaa cctgtctctt ttccgcttcc tcgctcactc agtcgctacg ctcctgccgt
     8941 tcggctgcgg cgagcggacg gggcacacag acagaaaagt catcgccacc acccgggaaa
     9001 ggaaaagaac agacgggaga aggccacggc gttgccgttt ttccataggc tccgcccccc
     9061 tgacaagcat cacagaaacg gacgctcaaa tcagcggtgg cgaaacccga caggactata
     9121 aagatcccag gcgtttcccc ctggtagctc cctcgtgcgc tctcctgttc ctgcctttcg
     9181 gtttaccggt gtcattccgc tgttatggcc gcgtttatct cattccacgc ctgacactca
     9241 gttccgggaa ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg
     9301 actgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaatg
     9361 cgccactggc agcagccact ggtaactgat ttagaggaga tagtcttgaa gttatgcgcc
     9421 agttaaggct aaactaaacg aaaagttttg gtgactgcgc tcctctaact agccagttac
     9481 ctcggttcaa agagcgccaa ctcttggaac cttcaaaaaa accgcccgcc aaggcggttt
     9541 tttcgtttac agagcaagag attacgatgc caaagaaagg atctcaagag gatcctgttc
     9601 catatacctt cccctgaagg gaaaccagcg acgccagtga actaacagaa aacaaaagca
     9661 tttgatctta ttaaaaaaaa tgaatataga gtaagatttt acacaatcag tggctttgtt
     9721 gaataaatcg aaattttggt aacctgaaga atcagatcac gcattatcct gactatgcag
     9781 gcagttccgt ggcaaagcag aaattcaaaa tcaccaactg acgcaactac aacaaggccc
     9841 tgattaatcg tgactccatc actttctggg ttgatgatga ggcgactctg gcctggtatg
     9901 agtcagcaac gcctgcatcg cggggacaac ctcagcgcta tacagcccct gccatcacaa
     9961 ccttcctggt gattaaacgt gtattccggc tgaccctgcg aaccgctcag ggctttattg
    10021 attccatttt tgccctgatg gatgtcccgc tgcgctgtcc ggattacacc agtgtcagca
    10081 agcgggcaaa gtcggttaac gtcagtttca aacgcccacc cggggtgaaa tttcgcatct
    10141 ggtgattgat tccacagggc tgaaagcctt cggtgaaggt gagtggaaag tcaaaaagca
    10201 cggcaaagag cgccgtcgta tctggcgaaa actgcatctg gctgttgacg cgagcaggca
    10261 tgaagccatc tgctcggacc tgtcactgaa caacgtcacg gacgctgaag ctttcccggg
    10321 gcttatccgg cagacccacc gaaaaatcag gtcagcggcg gcggacggcg cttacgatac
    10381 gcagcgatgt catgacgaac tgcgtcgcaa gaaaatcagc gcgctcattc ctccccgaaa
    10441 gggagcgggc tactggccgg gtgtgggaac agccctgcga aaccttgcag cagcgttcac
    10501 gcattacaat gaaaatcacc cgcccagcgc gctgggatat cactctccga gagaataccg
    10561 gcagctgcgg gcatcgttaa cttaagatac aaaagctgtc cggagatggc gggtcaagat
    10621 cactcaggcc gagatccgcc gtctccagaa agagctgaaa cgggttaccg atgaacggga
    10681 catattaaaa aaagccgcgg cgtacttcgc aaaactgtcc gactgaggta cgcctttatc
    10741 cgtgacaact cctgttgctg gcctgttcgc ctgctctgtc gggtgctgga tgttcatccc
    10801 agtggttttt acgcctggct tcagcagccg cattcacaac gccatcaggc agacctgaga
    10861 ctgacaggac agattaaaca gttctggctg gaatcgggat gcgtctatgg ttatcgcaaa
    10921 atccatctgg atctgcgtga cagcgggcaa cagtgcggaa taaacagagt ctggagactg
    10981 atgaaacgtg tcggaataaa ggctcaggtc ggataccgaa gcccgcgggc acgtaaaggc
    11041 gaggccagta tcgtgtc
//
