LOCUS       NZ_CP087610             5596 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01304 plasmid
            pDD01304-4, complete sequence.
ACCESSION   NZ_CP087610
VERSION     NZ_CP087610.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822729
            Assembly: GCF_023066605.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5596)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066605.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:08:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,916
            CDSs (total)                      :: 5,795
            Genes (coding)                    :: 5,630
            CDSs (with protein)               :: 5,630
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 59 of 165
            Pseudo Genes (incomplete)         :: 110 of 165
            Pseudo Genes (internal stop)      :: 18 of 165
            Pseudo Genes (multiple problems)  :: 21 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087610.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5596
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01304"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01304-4"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            complement(join(5576..5596,1..525))
                     /locus_tag="LMH54_RS29430"
                     /old_locus_tag="LMH54_29430"
     CDS             complement(join(5576..5596,1..525))
                     /locus_tag="LMH54_RS29430"
                     /old_locus_tag="LMH54_29430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023317533.1"
                     /translation="MFFKRYLTEKEIYKAALPIVRLQGRVNTSLRFLSKYSDVNKNGLI
                     ALHLNLLHFISYNIHGVESLKITQAGINKAVQQSPHADEIKNIAQAIGRKGPEYIEGLI
                     RNPWGKKNDNPLIFIMSIEESIKETLNSGYMLYRVAPITEGEAAAFMDVLKQLLLFEDN
                     FRLFGRDLVYKIEKLKIR"
                     /name="hypothetical protein"
     gene            complement(551..877)
                     /locus_tag="LMH54_RS29435"
                     /old_locus_tag="LMH54_29435"
     CDS             complement(551..877)
                     /locus_tag="LMH54_RS29435"
                     /old_locus_tag="LMH54_29435"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_050516389.1"
                     /translation="MRERTNIMLDIGLKQSLQRLSNKTKMSLSDLINQRLSGSLSVEER
                     IGEKPEWMRNLDPYLANHFSNIELPLPSPLKEYQEKMDEIDSIINDMMVLKEYFRAKFY
                     DEIK"
                     /name="hypothetical protein"
     gene            complement(1235..1393)
                     /locus_tag="LMH54_RS29645"
     CDS             complement(1235..1393)
                     /locus_tag="LMH54_RS29645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071596352.1"
                     /translation="MHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWIYTREIL
                     RGLEVGA"
                     /name="replication initiation protein"
     gene            2033..2209
                     /locus_tag="LMH54_RS29440"
                     /old_locus_tag="LMH54_29440"
     CDS             2033..2209
                     /locus_tag="LMH54_RS29440"
                     /old_locus_tag="LMH54_29440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197757.1"
                     /translation="MQKPKKKPLVVKKRSWHPAVMANLMRQAQEEDYRREQMAELERSA
                     RSHFADIGGEDGG"
                     /name="hypothetical protein"
     gene            2199..2543
                     /locus_tag="LMH54_RS29445"
                     /old_locus_tag="LMH54_29445"
     CDS             2199..2543
                     /locus_tag="LMH54_RS29445"
                     /old_locus_tag="LMH54_29445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_062955149.1"
                     /translation="MAVKRTRFLGIRVTDGEYQQLLERCNGRQLAVWMRETCLDTRPAR
                     SLRLPSIDPVLLRQLAGMGNNLNQIARKINGGQWSGADRVQVVAALMAIDAELERLRHA
                     VLEKGADDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2752..>3186
                     /locus_tag="LMH54_RS29450"
                     /old_locus_tag="LMH54_29450"
                     /pseudo=""
     CDS             2752..>3186
                     /locus_tag="LMH54_RS29450"
                     /old_locus_tag="LMH54_29450"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3221..3730
                     /locus_tag="LMH54_RS29455"
                     /old_locus_tag="LMH54_29455"
     CDS             3221..3730
                     /locus_tag="LMH54_RS29455"
                     /old_locus_tag="LMH54_29455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_032433972.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVRAELSESG
                     KRISAAIRAHEQGMSAAMQSNRLSVMRMVGRTWLTITMVSGLLFASLSGVLWYQGSLIA
                     SNLAEIDRQNAALSKLNAKTWGVTYLEDSNGRFLVLPKGTAVDRTQSWTVGNGRSKQNA
                     LRLVKE"
                     /name="MbeB family mobilization protein"
     gene            3737..3949
                     /locus_tag="LMH54_RS29460"
                     /old_locus_tag="LMH54_29460"
     CDS             3737..3949
                     /locus_tag="LMH54_RS29460"
                     /old_locus_tag="LMH54_29460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021527181.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFGEWRKMSGLMQRENA
                     ALSERVTRLSQQVERLSGQLQRLSR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4325..4453)
                     /locus_tag="LMH54_RS29545"
     CDS             complement(4325..4453)
                     /locus_tag="LMH54_RS29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000523570.1"
                     /translation="MGLKPGPKRIADSTGEPDKRQRDNKKTPGNTDKLKPSKSSKK"
                     /name="hypothetical protein"
     gene            4616..4840
                     /locus_tag="LMH54_RS29465"
                     /old_locus_tag="LMH54_29465"
     CDS             4616..4840
                     /locus_tag="LMH54_RS29465"
                     /old_locus_tag="LMH54_29465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6290 family protein"
                     /protein_id="WP_000879771.1"
                     /translation="MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYL
                     SAETAARVRRGDEAVHSSEDVRKSLGLDD"
                     /name="DUF6290 family protein"
     gene            4824..5090
                     /locus_tag="LMH54_RS29470"
                     /old_locus_tag="LMH54_29470"
     CDS             4824..5090
                     /locus_tag="LMH54_RS29470"
                     /old_locus_tag="LMH54_29470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262326.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004197762.1"
                     /translation="MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGK
                     PLKGELGEFWRYRVGDYRVLCEIRDDELVILAATIGHRREVYD"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5160..5510)
                     /locus_tag="LMH54_RS29475"
                     /old_locus_tag="LMH54_29475"
     CDS             complement(5160..5510)
                     /locus_tag="LMH54_RS29475"
                     /old_locus_tag="LMH54_29475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004197756.1"
                     /translation="MYWLIAFLAFIFPGRSFADGYTAYYRCNGTQEASLTVKDNKLSMV
                     VGLSKFNRQLDTYRFKEKGIEVVMQRAASDDNSEAIMSFIMPDVNNMTLLVKNNGYIIV
                     KTTCWLTKTDKQ"
                     /name="hypothetical protein"
     Typing          1868..1947
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="90.361"
                     /coverage="97.0"
                     /evalue="1.1100000000000001e-21"
     Typing          complement(1285..1682)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.526"
                     /coverage="91.0"
                     /evalue="3.03e-139"
     Typing          2534..4065
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.804"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tattttataa accaagtctc taccaaaaag gcgaaaatta tcctcaaata ataataattg
       61 tttaagaaca tccatgaaag cagctgcttc accctcagtt atgggagcta ctctatataa
      121 catataacct gagttaaggg tttcttttat actttcttca atgctcatta taaatattag
      181 tggattatca tttttcttcc cccatgggtt tctaattaat ccttcgatgt actcaggtcc
      241 tttgcgtcct attgcctgcg caatattttt tatttcatct gcgtgagggc tttgttgtac
      301 agctttgttt atgcctgctt gagtgatttt cagagactct acaccgtgta tgttgtatga
      361 gataaaatgt aataaattaa gatgcaatgc aattaatcca tttttgttta catctgagta
      421 tttggataaa aaacgtaatg aagtgttaac tctcccttga agcctaacaa ttgggagtgc
      481 tgccttgtat atttccttct ccgttaaata ccgtttaaaa aacataattc tccgcctccg
      541 tatagcgtgc ctattttatt tcgtcgtaaa acttagctct gaaatattcc tttagaacca
      601 tcatgtcatt aataattgaa tctatctcat ccattttttc ttggtattct tttaatggtg
      661 aagggagcgg taactcaata tttgaaaagt gattggcgag atagggatca aggtttctca
      721 tccattcagg cttttcgcca atgcgttctt caactgaaag tgaacctgat aatctctggt
      781 taataagatc acttagactc attttggttt tattcgaaag ccgttgtaag gattgcttta
      841 atcctatatc tagcatgata tttgttcttt ctctcattac ccccccttgc tattgcgttt
      901 tttgttgcgc acaaagctac cattgcgcat taagaaacgc aataaaaaag gagggaggga
      961 gtggtttgta catgatgtgc agtgcgctga gggcatcagt ggcgggtgtt tttccgtggg
     1021 atggatggag ctccggggat aacagaactg acgatcattt aaaggatctt cttgagatcc
     1081 tttttttctg cgcgtaatct cttgcactgt aaacgaaaaa accgcctggg gaggcggttt
     1141 gatcgaaggt taagtcagtt ggggaactgc ttaaccgggt aactgggtat acgagaccgc
     1201 agataccaaa actgttcttt cagtgtagcc gcagttaggc acccacttca agacctctca
     1261 atatctctct cgtatatatc cagttaccaa tggctgctgc cagtggcgtt tttgcgtgtc
     1321 cgtccgggtt ggactcaaga tgatagttac cggaaagggc gcagcagtcg ggctgaacgg
     1381 ggggttcgtg catacagccc agcttggagc gaacgaccta aaccgagccg agataccagc
     1441 agcgtgagct atgagaaagc gccacgcttc ccgaaaggga aaaaggcgga acaggtatcc
     1501 ggtaaacggc agggtcggaa caggagagcg cacgagggag ccgccggaga gaaatcaccg
     1561 gcatctttaa gtcctgtcgg gtttcgtcct cctctgactt gagcgtccat ttctgtgatg
     1621 ctcgtcaggg gaggcggagc ctatggaaaa acggcaacgc cgcatccttt tctccggccc
     1681 tttctgattt gagcgccttt gctttatccc gttatccgtg agtcggtcac gcccgccgca
     1741 gccgaacggc aggagcgcag ctaccgagtg agcgaggagg cgtcatttca tctgtccgtg
     1801 catggtattg cggacgctcc tgagccacat atcacacctg ccacatgagg cactttcatg
     1861 aatcgtgctc tcatgccaac atagtaagcc agtatacaca gcgtaaacgc tgtgactggt
     1921 tcagggctgc gccccgaaac ccgctaacac cactgacgcg ctggcgcttg tccaacgccg
     1981 gagcgacccg gaaaactttt ccgtgccgtc cggcgtttgc gaaggaggga taatgcagaa
     2041 gccgaagaag aagccgctcg tcgtaaaaaa aagatcctgg catccggcag tgatggcaaa
     2101 tctgatgcgt caggcacagg aggaagatta ccgccgggag cagatggcag agcttgagcg
     2161 ttcagcccgg tcacattttg cggatatcgg aggagaggat ggcggttaag cgtacccgtt
     2221 ttttaggtat ccgggtgacc gatggggagt accagcagct gctggagcgc tgcaatggaa
     2281 ggcaactggc ggtgtggatg cgggaaacct gtctcgatac gcgcccggcg cggtcgttgc
     2341 ggctgccgtc catcgatccg gttttgctgc gccagcttgc cgggatgggg aacaacctca
     2401 accagatcgc ccggaaaatt aacggcggcc agtggtcagg cgctgaccgg gttcaggtgg
     2461 tggccgcgct gatggccatt gatgccgaac tcgagcggct gcggcatgcc gtgctggaaa
     2521 agggggcaga cgatgatcgt taagtttcat ccgcgagggc gaggcggcgg tgccgggccg
     2581 gtggattatc tgctggggaa agaccgccag cgcgacgggg ccatcgtcct gcagggaaag
     2641 ccggaggagg tccgggagct tatcgacgcc tcgccctacg ccaaaaagta cacctccggc
     2701 gtcctgtctt ttgctgaaca ggatttaccg cccggccagc gtgaaaagct gatggcgagc
     2761 ttcgagcggg ttctgatgcc cggactcgat aaagaccagt acagcgtgct gtgggttgaa
     2821 caccgggaca aggggcggct ggaactgaat tttctggtac cgaacacgga actgctgacc
     2881 ggcaggcgtc tccagccgta ctacgaccgg gcagaccgtc cgcgcatcga tgcgtggcag
     2941 accgtggtga acgggcgact ggggctgcac gacccgaacg caccggagaa ccggcgcgcg
     3001 ctggtgacgc catcttcgtt acccaaaacg aagcaggaag ccgcagaggc tattacgcgg
     3061 ggcttactgg ccctcgcctc gtcaggggag cttaaaacgc gtcaggacgt cactgaggcg
     3121 ctggaaagcg caggttttga ggtcgtgcgc accacaaaga gcagcatcag cattgccgac
     3181 ccggacggag ggcgaaacat ccgacttaag ggagccatct atgaacagtc ttttaacgct
     3241 ggcgaaggac ttagagcaga aatcgaaagc gcagcagcag agtaccggcg aaatgctgaa
     3301 agccgcattc agcgagcacg agaagtctgt cagagcggaa ctgagcgaaa gcgggaagag
     3361 aatcagcgcc gccatccgcg cccacgagca gggtatgagc gcggccatgc agtcgaaccg
     3421 cctgagcgtg atgcgtatgg tcgggcggac atggctgacc atcacgatgg tgtcaggact
     3481 gctgttcgcc agcctgagcg gggtgttgtg gtatcagggg agcttgatag cgtcaaatct
     3541 ggccgaaatc gaccggcaga acgcagcgct gtcgaagctg aacgcgaaga cctggggcgt
     3601 gacgtacctg gaagacagca acggacgttt tctggtgctg ccgaagggga cggccgtaga
     3661 caggacgcag agctggacgg tcgggaacgg gcggtcgaag cagaacgcgc tgagactggt
     3721 gaaggagtaa gtgccgatga ccgagctgga aaaacagttg ctgagcgcat tagagcagct
     3781 acagcaggac tactcgaaaa ggctggacga gtgggagagc gccttcgggg aatggcggaa
     3841 aatgtcaggg cttatgcaac gggagaacgc ggcgctgagc gagcgcgtga cgcgcttgag
     3901 tcagcaggtg gaacgcttga gcgggcaact gcagcgtttg agccggtagt gcagcgtcat
     3961 gagatggccg tggccgcaga acgggcgcac gagcaacggc agcacgaaaa ggaactgacg
     4021 gaagcgcgtt cccggcagcg aagctatgac ggtccgtcgc tgggctgaaa tgcaggaaaa
     4081 cagggttctc atcgggcatt tacgctgaaa ccggatgtca ttcacctggc tgcatggcta
     4141 tgcagccagg taaaatttat ctgtcgcgaa cggggcggtt tatcgggtgg tttgttgccg
     4201 gttttacctg actgccaccc cgttcgcggc gaacccgtcc ggggcggtgc gggcaacggg
     4261 tgttatgttt aatcagcgtg aacggcagat acaaaaaagc cccggagggc ttttaactac
     4321 tgacttattt ttttgaagat ttgctgggtt tcaatttgtc agtatttccc ggcgtttttt
     4381 tattgtcacg ctggcgttta tcaggttccc ctgttgaatc ggcaattcgt ttcggacccg
     4441 gcttaagacc cataatcgtt atcctttcaa tagttaagag gaaagccctc aagctagata
     4501 tattgaccgc gttacggatt atcaagaggg gctaacaaga ttattttttg ttcttttagg
     4561 ttgattgtgg tactgttgat atacatgtat agcaaatggt gttcgggagg tttatatgct
     4621 tgctatccgg ttatctgatg agattgagtc ccgtctggac tcgctggcga agcaaaccgg
     4681 cagaacaaag acgttttatg cgcgggaagc aatactggcg catctggaag acctggagga
     4741 ttattatctt tcagcagaaa ctgctgcacg cgttcgccgt ggtgatgaag cagtgcattc
     4801 gtctgaagac gtgaggaagt cacttggtct ggacgattaa ctattccgat cgggcgctca
     4861 aatcgttacg caagatggac aaacagaacg cacgacggat tgtggatttt atgagtttac
     4921 gcattgcagt tgctgccgat cctcgccagt cagggaagcc gctcaaaggt gagctgggcg
     4981 agttctggcg ctatcgggtg ggagattatc gcgttctgtg tgagatccga gatgacgagc
     5041 ttgttatcct tgccgccacg attggacatc gccgcgaagt ttacgactga agcccgctat
     5101 gcgggctttt ttgatgccga caacaggtat tatggtaaat actcgactaa ctacgtgttc
     5161 tactgtttgt cggttttagt taaccagcag gttgtcttaa cgataatgta gccgttattt
     5221 ttaactaata aggtcatatt gttaacgtca ggcataataa aagacattat ggcctcagag
     5281 ttatcatcag aagcagctct ctgcattact acttcgattc ccttttcttt gaatctataa
     5341 gtatccagtt gcctattaaa cttagaaaga cctacaacca tacttagttt gttatcttta
     5401 acagtaagag atgcttcctg agtaccatta catcggtagt aagcggtgta accgtcagca
     5461 aaagatcgac cggggaatat aaatgctagg aacgcaatta accaatacat agttgagaat
     5521 cttggtatta cacatttcac tatcctctct ttcatttttc gttcaccata ctttgttatc
     5581 tgatttttaa tttttc
//
