LOCUS       NZ_CP087609            10062 bp    DNA     circular CON 08-JUN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain DD01304 plasmid
            pDD01304-3, complete sequence.
ACCESSION   NZ_CP087609
VERSION     NZ_CP087609.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22822729
            Assembly: GCF_023066605.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10062)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Insertion sequence medicated mrgB disruption is the major mechanism
            of polymyxin resistance in carbapenem-resistant Klebsiella
            pneumoniae isolates from China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   Jiang,J., Su,J., Xu,X. and Wang,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-NOV-2021) Institute of Antibiotics, Institute of
            Antibiotics, No. 12 Wulumuqi Road, Shanghai, Shanghai 200000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-AUG-2021
            Assembly Method                   :: Unicycler v. v0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200x
            Sequencing Technology :: Illumina+Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023066605.1-RS_2024_06_08
            Annotation Date                   :: 06/08/2024 04:08:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,916
            CDSs (total)                      :: 5,795
            Genes (coding)                    :: 5,630
            CDSs (with protein)               :: 5,630
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 165
            CDSs (without protein)            :: 165
            Pseudo Genes (ambiguous residues) :: 0 of 165
            Pseudo Genes (frameshifted)       :: 59 of 165
            Pseudo Genes (incomplete)         :: 110 of 165
            Pseudo Genes (internal stop)      :: 18 of 165
            Pseudo Genes (multiple problems)  :: 21 of 165
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087609.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="DD01304"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pDD01304-3"
                     /geo_loc_name="China:Shanghai"
                     /lat_lon="39.87 N 116.28 E"
                     /collection_date="2017-12-01"
                     /collected_by="Jiachun Su"
     gene            join(9905..10062,1..214)
                     /gene="tolA"
                     /locus_tag="LMH54_RS29365"
                     /old_locus_tag="LMH54_29365"
     CDS             join(9905..10062,1..214)
                     /gene="tolA"
                     /locus_tag="LMH54_RS29365"
                     /old_locus_tag="LMH54_29365"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            211..576
                     /locus_tag="LMH54_RS29370"
                     /old_locus_tag="LMH54_29370"
     CDS             211..576
                     /locus_tag="LMH54_RS29370"
                     /old_locus_tag="LMH54_29370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            751..1029
                     /locus_tag="LMH54_RS29375"
                     /old_locus_tag="LMH54_29375"
     CDS             751..1029
                     /locus_tag="LMH54_RS29375"
                     /old_locus_tag="LMH54_29375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1017..1331
                     /locus_tag="LMH54_RS29380"
                     /old_locus_tag="LMH54_29380"
     CDS             1017..1331
                     /locus_tag="LMH54_RS29380"
                     /old_locus_tag="LMH54_29380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1495..1923
                     /locus_tag="LMH54_RS29385"
                     /old_locus_tag="LMH54_29385"
     CDS             1495..1923
                     /locus_tag="LMH54_RS29385"
                     /old_locus_tag="LMH54_29385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            1949..2128
                     /locus_tag="LMH54_RS29390"
                     /old_locus_tag="LMH54_29390"
     CDS             1949..2128
                     /locus_tag="LMH54_RS29390"
                     /old_locus_tag="LMH54_29390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2155..2685)
                     /locus_tag="LMH54_RS29395"
                     /old_locus_tag="LMH54_29395"
     CDS             complement(2155..2685)
                     /locus_tag="LMH54_RS29395"
                     /old_locus_tag="LMH54_29395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(2692..3423)
                     /gene="mobC"
                     /locus_tag="LMH54_RS29400"
                     /old_locus_tag="LMH54_29400"
     CDS             complement(2692..3423)
                     /gene="mobC"
                     /locus_tag="LMH54_RS29400"
                     /old_locus_tag="LMH54_29400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(3423..5387)
                     /locus_tag="LMH54_RS29405"
                     /old_locus_tag="LMH54_29405"
     CDS             complement(3423..5387)
                     /locus_tag="LMH54_RS29405"
                     /old_locus_tag="LMH54_29405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(6873..7022)
                     /locus_tag="LMH54_RS29410"
                     /old_locus_tag="LMH54_29410"
     CDS             complement(6873..7022)
                     /locus_tag="LMH54_RS29410"
                     /old_locus_tag="LMH54_29410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(7107..7364)
                     /locus_tag="LMH54_RS29415"
                     /old_locus_tag="LMH54_29415"
     CDS             complement(7107..7364)
                     /locus_tag="LMH54_RS29415"
                     /old_locus_tag="LMH54_29415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(7374..9059)
                     /locus_tag="LMH54_RS29420"
                     /old_locus_tag="LMH54_29420"
     CDS             complement(7374..9059)
                     /locus_tag="LMH54_RS29420"
                     /old_locus_tag="LMH54_29420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            9386..9631
                     /locus_tag="LMH54_RS29425"
                     /old_locus_tag="LMH54_29425"
     CDS             9386..9631
                     /locus_tag="LMH54_RS29425"
                     /old_locus_tag="LMH54_29425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     BGC             2374..10062
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          6190..6770
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.603"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2696..3423)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 gatgtttgaa ccagaggcct atgcgggaaa aatgtgcacc gtgcggatat ccatacaacg
       61 tgatggcact ctgaacagcg ccattgctga aggaggagat ccgaaactct gtaaagcagc
      121 aatatcagcc gttacccacg ccaaaattcc gccggcaccg gatgaaaata cctggcaaat
      181 attcaaaaac gcccctctgg atttcagacc atgaaagctt ttactctgac agtgctggcg
      241 gccatgttcc tcactccggt acatgcgata ccactaaaac ccgtaaccac actgcagcca
      301 acctacccgg tacaggccgc agcaaaacgc atcgaaggtc aggtgaccgc cacgtttgat
      361 attctggacg atggacgagt ggacaacgtg tcgcttaccg gctgtcacct tttttttcgg
      421 gaaacgctga cggcaatgag gcactggcta tatcccgcag ggcacccggc aatgcgacag
      481 gttataaatt ttgaattcag tctgaaaaat gacggcttac aggatccgcg accagtcacc
      541 acccaaaagc tcaatgatga tggttgccgc cagtaacagg tcctttaacc cgcaggctgc
      601 aatagtcctg gatgatgagt gagcctgtag cagccctgat tagtggaagc gaagctgaaa
      661 aatgggcagc aaaagccgta tttctggcaa atagcgccgc cacggattaa agtagtcatg
      721 tgtaatacat atgactacaa aggggctttt atgtcatcag caacatccat caggctcgat
      781 gaagaactta aggaccggct gaaaacgctg gccgatgacc ggcaccgctc agcgcacgcg
      841 ctaatgctgg aggcaatcac cgaatacatc gaccgggaag agaagcgcag ccagtacctg
      901 cgggacggac aggccgcatg gcagcactat caggaaacgg gtctgcacct tactgcggaa
      961 gaagcggaag cctggataag tacctgggga actgagaacg agcaggatgc tccgccatgc
     1021 cacaggtaac gatttccgcg ctggcacagc gcgatttaca acgccttcag gactttctca
     1081 aaaccaaaaa tcggctggca gccagaaaag gaggtgaggt gatcgtccgg gctatccagc
     1141 aactgaaaac attgccagac attggccgcc cggtgccgtt tctgccgctg gaatataagg
     1201 aactggtgat cgggtttggc gacagtggct atgtgatgct ctaccgccac gaccgggaaa
     1261 tggaccagat tgtgatcgtc accgtcaggc atcagaaaga atccgggtat ccgggggcag
     1321 acagcctcta ataaccgatg acgggggcgc aattcattgc gtggccgtcg ccggtttgac
     1381 aagcgcagcg cgtcagaaaa ccacatcctg aacggaaaaa cgggtaaaat tcagcgatac
     1441 atttaccgtg agaaattaca cacctttcac acccgataaa atcaggggta tacagtggcg
     1501 aaaacattgc tcaacctttc acaggcagca caagccgcgg gcattaccag aagaacgcta
     1561 tacaatcatg taaaacaggg aaaggttacc gtgtcacgtg acgggaaaaa caaccctgtc
     1621 gtggatgtgt cagaactgat ccgtgtatac ggaaatgtga atatccctga gaaacaaatt
     1681 cccggcattt cacaccgtga aaatacacaa aaaaatttcc cacaggaaca gcttctggca
     1741 atgcaaaagg aactggccga tctccggcag gctgtcaccc tgatgctgga agataaaaca
     1801 tcccgggaag aagaacgccg ccagcacgat gatgagcgcc ggaaactcca ggccgaagtg
     1861 gacagattga caacagaact gacacagaag aaaaaacgct tctggtccgg ctggtttagc
     1921 tgagaactta ccgaagggaa tggagaacat gacgaagcag gaaaaaactg ccctcaatat
     1981 ggctaaattc attaaggatc agtcgctgct gttgctggaa aagctgaacg agctggacat
     2041 ggataccccg gccgacatgt gcgaacggct tcacgaggat gcggaaacac tctggcaggt
     2101 gatgcgggat aagctcgggg aagaataaca gcaccggcca tgccggtgct ggcgttattt
     2161 tttgccgaac ggccaccacc ggcgggcggg tttgtcctcc ggcagttcag gcaccggcac
     2221 gttaaaatag cggctgttat attcccgcat cagctccgct ttcagcttct cgttcaccgc
     2281 gctttccccg tgccgctcgc tgaccacctc aatcatcgcg ttaatctgct ccggtgagca
     2341 cttcccggca tcaaccacct gcacaccgtt ctcgatggcg aaccgctcca cctttcgata
     2401 gaggtaatca cacagcttca ttcctgtggg tttgtcccgt tcccattgcg gattttcacc
     2461 ggtgaaaaag tacgtggctt tttcgtcgct gttgagccgc tcgtcataca tcccgatgga
     2521 tttcatcatc gccagatgct gggcccggtc gacaaaacag aaaacatacg ccccggtctg
     2581 ccaggcgagc accaccatgt cttccagatg ctcgctggtt ggcataatgc agcgctcgcc
     2641 gtctttgtct tccacgtcct caataaatac catgtacagc gccataatcc ctcactccgt
     2701 tgtcataaat ttaaagcacg cgaacagcct ctcccggtcc agctccaccg ttttgcgggc
     2761 ctcgccggtc tgtggattca cgatttcatc caccgccaga atcgcccgct gtacccggac
     2821 aagcgtcggc cggtcagggc agatgtacca gatttcatcg atgccgttcg cccggcggtt
     2881 gaacagatac ccggacacca cttcctgata ccgcctgcgg ctcttgacgg tgcgctctac
     2941 ctcaaatgcc accgttctgc cgtccagctc cgccagcgcg tccggcactt tcatcccttt
     3001 cagggccata cggtgcaggt accgccagcc ggtggccccc cggccctcca tggagaggcg
     3061 cagggactgc acggccagcc ggtgcggcag ctgcgccgcc gacacccttg acggctgaaa
     3121 cggaataacg tccgtcagcg gctcgtcctc gtcgaacgag aacgccaccc cgtgcggcgt
     3181 cagcccccac acgctgacct gacggccgtc aaccgggtat ttcgcccgga ccagcagccc
     3241 tttcgccacc agactgtcca ggaagcggta aaccggctgt accgccgcaa tccccagccg
     3301 tcgcgcaacc agctccgccg tggaacacag ctcttcccgc agccagcgca gggtcagccg
     3361 tgttttctcc gcgttccgcg cctgccgctt cgcgtggctg acgttgtact gctccagtgt
     3421 catcagtaaa gccccgcaaa atcatcatcc gcttttggtc cgccgccgtc ggccggaggc
     3481 aggtactccg ccagcgggtc cgttttcgcc agcaccggga aggttttcag cgaaatttcc
     3541 cggcgctcaa cccgcagcgg gctgctgaac gccagccgcg ccacgcccag cccggtcatc
     3601 accgcgaacc cgtcaggcag gtgttgcagg gtgttcacgt caaacagcgg ccgcgcctcc
     3661 ttctggatga agctgtcgcc gctgatgtgc tccacgttcc ccgcctcccg gctggcccgg
     3721 cggcgctcca cgtccacccg gatctccccg gtctggcccg ccgcccactg cgcggactcc
     3781 tggctggccg cgcgatagaa ccagcggatc ggcgtgttat ccagtaccgt ggttttcaca
     3841 aaatcagcgg gcagatcctg cccgcactga ccgaagtcgc ccagcgactg gtgcgcgagc
     3901 agcaggttgc agtcccggct gcgcagcgtc cccagcgcgt tcagcacgta cttcgacagc
     3961 agatatttaa tctcatcgag catgatgctc gcgtgcggcc acctccggaa ctcatcccgc
     4021 gcaatgatga tttgcgcgca gcgggcaaac agcatcttct gcacccggat caccgcctca
     4081 tcgtccatcg agccgatgac gtacaggcag ccgccgccgt tgattatccc cgccacatcg
     4141 ataccgctgt ccgtctgcag gacgctcagg tcggccacgt tctccagctt ggtgatcagc
     4201 cccttcacgt cctttttcag ggcctccggc aacgcgtacg ccgccgccag cacctgctga
     4261 atgtttgccc cctgcgggaa ctgctccgca atcagcttcg ccgctttctg ctcgctgatg
     4321 cggtagtggt ccgccacgtc gccggtctca ctcaggttaa acccggcaac cagcagattc
     4381 ttcagggcgt actggtcgag atcgcggaac agattcagct gcgccggttt ccccggccgc
     4441 agatccagca gggtgaagtc cgggcagtgc gcccggaaga cgtgcggcgc ccacgcgtcc
     4501 cctttggggt cgaagtacac cacagcgtcc ccgaaatcgc gtacgcagcg aatgagcgcg
     4561 ttggtggcca tcgcgctctt gccgctcccg ggcaggccga ggatctgaat attggtcttg
     4621 cgccaggtat cacgagggac cagcaccggc tggccctgct catccttacc cagaaacacc
     4681 gcctgctgtt tccgggcctg ctccgtggaa aaatcggcca gcgcgttaac cggtaccggc
     4741 tccccgaaat gcaggtggcg gacgtctgtc atttctccgg tccgctggct gctgcgccgg
     4801 ctctcctgat tcagtttcgc gttcaccgcc tcgtcagcgg ccgcaaaatg accatcaagc
     4861 agcaggaacg ccagcagccg cgccagaagg acgccggcga caagccctcc ggcacaccag
     4921 gctatcccga acaggttagc cggctgcagc aggtaataat ctgcgttcgc atagaggtcc
     4981 gggcgcagga cggtttctcc ccagctctcg ataaacacgg cgacaaccag cccccccaga
     5041 agggccagac cggcagcgcc ggtcagccag tacgcgtagt tccagacatg tcgccgtgcc
     5101 ctgaccatcc gcccgggtga atccagcgag gccagcaccg aaaatggcac agtccggtta
     5161 ttatcgcgct tatagcgatc ggcatactcc cgccgcgcct tatggctcag ccccacaaaa
     5221 cgcattcggc cggcccagag ttcagccaga ccgaaaaaca ggccgataac cagaaaaaaa
     5281 ccggccagac tgtaatactc ccggtacagc cagctgaccc acgggtaaat ctgccgggcc
     5341 agctccggga tatgccactg ccaggcaagc gaatccgtat taaacatgca atctctctcc
     5401 ttcagacgac gggtttaccg gcgtgatgcg cagcccccgc tgcacaagcc gcggacatgc
     5461 cccaaggcat ggcggcggcg gtcacgtccg tggtgattgc gttcagcttt caccgcggag
     5521 gccagcctct ttccgcccca tgcggaaaag gcgcagcccc ggaggaacaa cgagcacgcc
     5581 ccagcgtgtg agaggaaaag gcggcacccc agtgcggtct tttccggtgt tccggccccc
     5641 gtcagagttc gccatagcga gcggctgacc acccgcaaaa tcaggagcag ctttgctgct
     5701 tcgtcaggtg cgtccagcac cgtggatttt tgtgggtggt caggcgtgag cctgacgggg
     5761 gccgggtgaa tttctggcgt ggccggcgtc cagccggaca gagccagaaa gaggggaaaa
     5821 gaccgtgcgt cggtggcgtc cagccagcgt aagcaaggtc ttttcccctg aagtcagcca
     5881 cgccccagcg gggatgactt gcatttctcc gcaaggagaa aaaagcaaca gagataggtt
     5941 aacgtaaaat gttcagcgaa aaaccgacat ggttcaccta tcctgataat tgatcgtcag
     6001 ggaatagaaa gacttaatca ggggacaata tcccacatca gcgcgtccgg tgaccgtctt
     6061 gcaggtgccg gggaaattgc gcctcctcgc tcactcggtc gctacgctcc gggcgtgaga
     6121 ctgcggcggg cgctgcggac acatacaaag ttacccacag attccgtgga taagcagggg
     6181 actaacatgt gaggcaaaac ggcagggccg cgccggtggc gtttttccat aggctccgcc
     6241 ctcctgccag agttcacata aacagacgct ttttcggtgc atctgtggga gccgtgaggc
     6301 tcaaccatga atctgacagt acgggcgaaa cccgacagga cttaaagatc cccaccgttt
     6361 ccggtaggtc gctccctctt gcgctctcct gttccgaccc tgccgtttac cggatacctg
     6421 ttccgccgtt ctcccttgcg ggaagtgtgg cgctttctca tagctcacac tactggtatc
     6481 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc
     6541 ccgactgctg cgccttatcc ggtaactttc atcttgagtc caacccggaa agacacgcaa
     6601 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
     6661 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
     6721 tgctgtgctc tgcgaaagcc agttacccgg ttaagcagtt ccccaactga cttaaccttc
     6781 gatcaaacca cctccccagg tggttttttc gtttacaggg caaaagatta cgcgcagaaa
     6841 aaaaggatct caagaagatc ctttgatctt ttctactgaa ccgcgatccc cgtcagttca
     6901 gaggaggacg atggtgccac cgttccgccc tgaacatccc ggatataatt tgcctgacat
     6961 gcggccagaa gaaagccagc aacaatcaat ataaaaagaa aaatagtctt tacctttttc
     7021 atatactttc cctgtattta aagactccgg cattcatccc tgaacaccgg aagtcagtat
     7081 ttcaaatcat ccttgataat tttttgttac cagttaccgt cccgataatc aaaagctata
     7141 taacaattat ccacatcagg ttttatttga tttttaaaaa atggctgtag taaaggaacc
     7201 cattctttct tcaaatcaaa ctcaccatta ttaatattat cctttaaagg cattcccaag
     7261 ctttcaatga cagaaccatc gtcaccaaag tctttagaat actcttcccc tttaaactct
     7321 tcagttttct tatcaaacca tgtcaaacgt aatttaagcc ccatatttac ctctcaaaga
     7381 tattttttaa tgttccgttt tggatctggc cctttaacct gcttacccgt tttgggatca
     7441 aatgcgccta ggtgttcgcc atcactggca cgataccctt caagctcacc gtgctgggaa
     7501 tcccattcat aaattttacg ttttttatcg ccataccatc gggcccgctt accaccacca
     7561 ccttgtttag gtgttttagg atcacctcgc tttaactctc caagtccctt tatgtcttct
     7621 gttttaggta ctggatgata atcatggccg tagtctttag tccccttgcg aggcttgttc
     7681 ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt tctccttttg tttccggctc
     7741 tccatcgccc cggtaagtgc agccttatct ctggataagg attcattcat tgcgtttaaa
     7801 gatgcctgag ccgcttttaa tttctcattc tgattctgaa tatccttatc aagatagcga
     7861 acctgatcct gccacccgga gccatattca tgaggcgggt tataagtcac aaaatcatct
     7921 ttatttgcct ctgcctcttt taccttttta tcggcttcct gaacagcagc ccttgcaccg
     7981 gggagcgcct tctctgtcga tgcaatgcgg ctgtttaagg tcgcaatatt tttatcttcg
     8041 gcatccagct cagctttcgc tttatcatag tctcttttca gcccctcttc cggatgagcg
     8101 gcgtcccatg cctgctggcg acgcttttct tcctcctgac gctgttttac ctgtgccggg
     8161 gtaagaacat ctgtcaccga cacataaacc ggcttctgtc cggtctcttt cgggaaacga
     8221 ataacggcct cacgggagtt accaccggct gtaaaaccag ccggacgtga atcacctttt
     8281 tcagcaacaa tgccttttgg cggggctttc gctgtcggaa cacctttcgg tacgctcacc
     8341 tgcagagacg ggagacccgg aatcgacaca ctgaataccc ccggacgttt tgtcggtttc
     8401 gcatccacca cagggacact cattggccga cctgcgacaa ccgcaatatg ctgccgctca
     8461 tccttcacca catccacaac ccgttgtctg acacgaaccg tcgcctggtc cagtggtaaa
     8521 gtacttgccg gagtctccgt caccgcatcg gccggtaatg acgtcataat ttttgacatc
     8581 attttcggat catcttttgc aatctcagaa ggcagcacac cgtagatcgc aatcccccac
     8641 agaccaaact taaacggccc cttcagggca gccagcacat cagcaacggc ggccgacaac
     8701 gcatcgccgg aaactgataa agccagtcct tcagcccccg gagtagccag ggcaggcaga
     8761 ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag agctaccgct atgattgccg
     8821 ctgttaccac cattaccacc gggtttactc ccgccgttac caaactcccc cggattatag
     8881 ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc ccggcccgct gtttggatcc
     8941 gtaccaccac ctgatgaagg gccaccttta ccagacgtac cgttcacatt cccactggcc
     9001 tgaccaccat tatgtcccag acctgaatta cccggacctc gaccgtctcc accactcata
     9061 ccgttttcct ctttgacatt taaaacaata aattaaagcc aacatactgt acatatacac
     9121 agtataatta ttaccttact cctcccgttt tcaggtgtca atatatgaga ttagttctca
     9181 tcttcaggca caaaaaagcg cccggagcca cctgtaagca gatcgcgtct tttcccgaaa
     9241 atttctcatg gcaacaaatt ttattattta agacattcat cacacttaca tttcagatgg
     9301 tatagacaca tctgaaagct aactggcatg aggagaggac ctgcggttaa actgttcggg
     9361 aaacctgaga cgcccggagg ccactatgac gcacctgaaa ctgactcccg ctgaagaaaa
     9421 gcgcctggcg gacgcctttg tcgctcaggc actggccatg ggcctgaaag ggaacaaaac
     9481 gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg cttcgtgcca ttcaggacga
     9541 acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa tacgaggcta cggaaaccac
     9601 cttcacctgg cagccacagc gccgccggta aggggtattt accataacgt cgcattgccc
     9661 gcaccgctca gacggtctga gggcgtttcc ggcggctacg ggataaaaca ggcaacaaac
     9721 cacccggaaa accgccggaa acgcgcccgg taaattgtaa cccgctgcat gcctatgcac
     9781 gcaactgcat ggcgggccgt ttggtgtaaa aacgatacga taagccgtgt ttatgtaaat
     9841 gaacgtggcc gtaaagagtg acaaatatac atgtaacatt ttttactgac aggtcatcaa
     9901 cccgatgcgt ttaataccct ttatcctgct ggcattgtgc agccccttac tcctgacggc
     9961 ctgtaagcat tccaccaccc agccgccgaa aatttatact ctggataagt catctaaaat
    10021 cagtagctac gctgcggcca tcaagaccgc tgttgaaatg ca
//
