LOCUS       NZ_CP087595             8571 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Acinetobacter baumannii strain SHOU-Ab01 plasmid pAb01-1, complete
            sequence.
ACCESSION   NZ_CP087595
VERSION     NZ_CP087595.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23235066
            Assembly: GCF_020985285.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   Yuan,L., Zhang,X., Wang,D., Lv,Y., Guo,Y., Wang,L., Liu,X. and
            Wang,H.
  TITLE     Characterization of a strain of Acinetobacter baumannii isolated
            from ascites of Andrias davidianus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   Yuan,L., Zhang,X., Wang,D., Lv,Y., Guo,Y., Wang,L., Liu,X. and
            Wang,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) State Key Laboratory of Pathogen and
            Biosecurity, Beijing Institute of Biotechnology, 20 Dongdajie Street
            Fengtai District, Beijing 100071, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 17-NOV-2021
            Assembly Method                   :: Canu v. v.1.6
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 272.0x
            Sequencing Technology             :: PacBio RS; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 02:03:33
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,753
            CDSs (total)                      :: 3,658
            Genes (coding)                    :: 3,567
            CDSs (with protein)               :: 3,567
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 50 of 91
            Pseudo Genes (incomplete)         :: 43 of 91
            Pseudo Genes (internal stop)      :: 16 of 91
            Pseudo Genes (multiple problems)  :: 14 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087595.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8571
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="SHOU-Ab01"
                     /isolation_source="Andrias davidianus"
                     /db_xref="taxon:470"
                     /plasmid="pAb01-1"
                     /geo_loc_name="China: Shanghai"
                     /lat_lon="31.23 N 121.47 E"
                     /collection_date="2021-07-11"
                     /collected_by="Xiaoyan Zhang; Lu Yuan"
     gene            93..308
                     /locus_tag="LOZ89_RS18630"
                     /old_locus_tag="LOZ89_18630"
     CDS             93..308
                     /locus_tag="LOZ89_RS18630"
                     /old_locus_tag="LOZ89_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004693381.1"
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]; GO:0003723 - RNA binding [Evidence IEA]; GO:0001072 -
                     transcription antitermination factor activity, RNA binding
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="WP_000071892.1"
                     /translation="MSNSNVVKGTVKWFNETKGFGFIQQESGPDVFAHFSEIASSGFKT
                     LFEGQQVEFSVAQGQKGPNAVNIVAI"
                     /name="cold-shock protein"
     gene            407..634
                     /locus_tag="LOZ89_RS18635"
                     /old_locus_tag="LOZ89_18635"
     CDS             407..634
                     /locus_tag="LOZ89_RS18635"
                     /old_locus_tag="LOZ89_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000921865.1"
                     /translation="MLKVNDLIAKSKNGTEIIVSLIPLHKMQNTRQGLKQIEVGKRVLL
                     ESGIEVDLNLDGRTFYTSLNQLFKLNHKVA"
                     /name="hypothetical protein"
     gene            701..898
                     /locus_tag="LOZ89_RS18640"
                     /old_locus_tag="LOZ89_18640"
     CDS             701..898
                     /locus_tag="LOZ89_RS18640"
                     /old_locus_tag="LOZ89_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000476222.1"
                     /translation="MFKQTLSVKDQFGVKHAIQATVDHEFANTQSHLNIKHITVDGEDI
                     RPSFEMLFQSTLSGKIFKII"
                     /name="hypothetical protein"
     gene            1085..1405
                     /locus_tag="LOZ89_RS18645"
                     /old_locus_tag="LOZ89_18645"
     CDS             1085..1405
                     /locus_tag="LOZ89_RS18645"
                     /old_locus_tag="LOZ89_18645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000897309.1"
                     /translation="MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGI
                     RKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAYPKSVKDNLTDKETSILRQLVKEQF
                     HG"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1398..1670
                     /gene="nadS"
                     /locus_tag="LOZ89_RS18650"
                     /old_locus_tag="LOZ89_18650"
     CDS             1398..1670
                     /gene="nadS"
                     /locus_tag="LOZ89_RS18650"
                     /old_locus_tag="LOZ89_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="WP_000369780.1"
                     /translation="MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTG
                     LTQAEFAARLHISARTLQNWEQGRRYPTGPAATLIRILDAHPTLI"
                     /name="nadS"
     gene            2288..2806
                     /locus_tag="LOZ89_RS18655"
                     /old_locus_tag="LOZ89_18655"
     CDS             2288..2806
                     /locus_tag="LOZ89_RS18655"
                     /old_locus_tag="LOZ89_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            complement(2994..3221)
                     /locus_tag="LOZ89_RS18660"
                     /old_locus_tag="LOZ89_18660"
     CDS             complement(2994..3221)
                     /locus_tag="LOZ89_RS18660"
                     /old_locus_tag="LOZ89_18660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004812234.1"
                     /translation="MHSFSVKELDQLIENDLKEKKKSSKLLIGYKLFNDLMNDPKFYAE
                     VTDSALSATKRKYKNLKIKITTDKYQLHFE"
                     /name="hypothetical protein"
     gene            complement(3609..4133)
                     /locus_tag="LOZ89_RS18665"
                     /old_locus_tag="LOZ89_18665"
     CDS             complement(3609..4133)
                     /locus_tag="LOZ89_RS18665"
                     /old_locus_tag="LOZ89_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008307689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_034684955.1"
                     /translation="MKSLTVLELSKLYNINRQTIYNNIKKGILSKNSDNKIDLAEAIRV
                     FGEPSKKLDVKESVKIDSPNSAEVLLLRQQIDMLKNQLDDAKERESFYQNQIETMQRLL
                     EAPKPKIQDPSELELPKSAPIVEMKEEVIVVPPKDDGLTTPENKRIPVPEHVEPEPKKR
                     GFLSRFFLPYG"
                     /name="plasmid replication DNA-binding protein"
     gene            complement(4212..5141)
                     /gene="repM"
                     /locus_tag="LOZ89_RS18670"
                     /old_locus_tag="LOZ89_18670"
     CDS             complement(4212..5141)
                     /gene="repM"
                     /locus_tag="LOZ89_RS18670"
                     /old_locus_tag="LOZ89_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131200.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_034684953.1"
                     /translation="MTDLVVKSNKLVQALQTLTLSETRLLQLAIVDARETGQGLSTDEP
                     LELNALRYAKAFNVSPDAAYLALIEAEDSLFKRQFTITNEDGTLTKSRWIQDANYRKGE
                     GRILVTLTRVVIEHVTKIDGFEQYFTSYHLKKTADFKSVYAVRLYELLMQWKSVGKTPV
                     YELNKFRSQLGIGVNEYTRMEAFKRRVLDIAVDQINEFSDITVKYEQHKKGRSISGFSF
                     SFKPKKATIRSIETNRNPNTTDLFSRMTDKQRHLFATKLSELPEMGKYSQGTESYPQFA
                     IRIAEMLQDSEKFQELFPYLQKVGYQAA"
                     /name="repM"
     gene            complement(5688..7124)
                     /gene="mobQ"
                     /locus_tag="LOZ89_RS18675"
                     /old_locus_tag="LOZ89_18675"
     CDS             complement(5688..7124)
                     /gene="mobQ"
                     /locus_tag="LOZ89_RS18675"
                     /old_locus_tag="LOZ89_18675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_059264791.1"
                     /translation="MAIYHFSVKTVARSAGRSATAAIAYRAGEKIYCEREGREHDYSRK
                     TGVEYKEIYLPEGAPEHLKNRERLWNEVEQRETRKNSTVAREFEIAFPSELNQEQRLAM
                     LKELCASIVDRHQVAVDACIHAPHTGSGSDERNYHAHILMSTRKLTPEGFTEKTRELDQ
                     KHSGEIEHWREHFADICNMHLDLAGYTARVDHRSYKDQENGLEATLHEGPKVTELRRKG
                     IETEISRSNDEIKQRNQAQLQYGKNMDLLIAENEIKLNTLKTEQQIQIKNSAKTPPIDE
                     KALFEEKQRETLGKVLNREISAKDANLDLEFMQDVFKKAENTLGKMQKHKNEFSQQLAQ
                     DIVKNRLKQSHDKLQSLVDQHNELTQNKPLLFGKKAWEAQRDEVYQEHKKLKGQHEHQK
                     KHGMKDLLENEKFKEHAWKQYQQQHPAKAKQYQTLYSTYQVIKKCVNEIKAEQQLKLRQ
                     EQQLKAQQHAPRMKSRGMSR"
                     /name="mobQ"
     gene            7335..7601
                     /locus_tag="LOZ89_RS18680"
                     /old_locus_tag="LOZ89_18680"
     CDS             7335..7601
                     /locus_tag="LOZ89_RS18680"
                     /old_locus_tag="LOZ89_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_188200041.1"
                     /translation="MSVTETLDSKIKAQEEKLKQLKAQRQAALARERAKEKEQARKDDI
                     RRKILIGSCMLKITEEDEQARAKLIAQMDKYLTDERDRKLFNL"
                     /name="mobilization protein"
     gene            7619..8338
                     /locus_tag="LOZ89_RS18685"
                     /old_locus_tag="LOZ89_18685"
     CDS             7619..8338
                     /locus_tag="LOZ89_RS18685"
                     /old_locus_tag="LOZ89_18685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001218546.1"
                     /translation="MALSQFDIIKSLGESIEWFEKELSWGMPIAELRHLTGRIGELYVA
                     MMTLGQLADSVNQRGYDVVSSTGQRISVKTVTTASHVDFNLKTIELVDRIIVLRINPED
                     LCIEVVYDLNMEQFQAKSREYKGKYILGIYNKILLANSKINIGLNIPTETELKVENNHE
                     LLILNNIFYDGYEIKQYENAKIEVYKDGEKQPSAKEILRQIALPLNVSLNNSAGNPKNT
                     QQLGDHVIKAIKNFRST"
                     /name="hypothetical protein"
     Typing          complement(4213..5141)
                     /name="rep_cluster_1259"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.28"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5692..7124)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.071"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttagatttta agtctcattc tttattattt tcaaagttat taatcagtca ataaaatgac
       61 taaaaaatta ttaaaaatta gtaggatata ttatgtcaaa ctcaaatgtt gttaaaggta
      121 ctgttaagtg gtttaacgaa actaaaggtt ttggtttcat tcaacaagaa tcaggtccag
      181 acgtttttgc tcatttcagc gaaatcgcga gttcaggttt caagacctta tttgaaggcc
      241 agcaagttga attcagtgta gctcaaggtc aaaaaggacc gaatgctgta aatattgttg
      301 ctatctaagt aataataagc agtaataaaa aagcactcat aagtgctttt tttaagcttt
      361 aaaatgtttt caaaattgaa aaaatctcta aaaaatagga tataagttgt taaaagtaaa
      421 tgatttaatt gcaaaatcta aaaatggtac tgaaattatt gtttcattaa ttcctctaca
      481 taaaatgcaa aatacacgtc aaggtttaaa acaaatcgaa gtaggtaaaa gagttctact
      541 ggaatctgga atcgaagttg atttaaattt ggatggccgt acattttaca cttctctaaa
      601 tcagctattt aaattaaatc ataaagttgc ttagcgataa atttttaaac atcaataatt
      661 tatgtgggat ttccactgca atttttttaa ggaaatatat atgtttaaac agactctcag
      721 cgtaaaagac cagtttggcg ttaaacacgc tattcaagcc acagtggatc atgagtttgc
      781 caacacacaa tctcatttga atataaaaca tatcaccgtt gatggtgaag atattcgacc
      841 tagttttgaa atgctttttc aaagtacctt aagtggaaaa atatttaaaa tcatctagaa
      901 atatagtttt tataaaatct atagtctgaa tgaaatatca ggacctactc gctatcatgc
      961 gggtttttta tacctaatat ttatatttgt aagcctttgc ttatgcgaaa cgagagttca
     1021 cagctctcgt ttttttattc taaaactgct aaaattctta tacgccattg acgtacttaa
     1081 acctatgtta ttcattgaaa ccagtatttt tactaagcaa atcaaagagc ttgtgagtga
     1141 tgaagagtat cgtcagctcc agcaagatct cttggtacag cctgataagg gcgacttaat
     1201 caagaatggt ggtggaattc gtaaagtacg ttgtgctcag ggcaataaag gcaaaagtgg
     1261 cggtatccgt gtgatctatt attgggttac agaagatgat cagatctttt tcctggtggc
     1321 ttatccaaag tctgtgaaag ataatctgac agacaaagaa acctcgattc tacgtcaatt
     1381 agtaaaggag caatttcatg gataacaact tatttgatga cctggttgct tcaatcaaag
     1441 aagctggtgc catcaagcgt aaggagataa aggcaagtcg agtcacagag cttgaattac
     1501 cggatattaa agaagtacgt gagaaaacag gcttaaccca ggctgaattt gctgcacgtt
     1561 tgcatatcag cgcgagaacc ctgcaaaact gggaacaagg ccgtcgctat ccgactggtc
     1621 ctgcagcgac tttaattcgt attttagatg ctcatccaac tttaatttaa gaagtttaga
     1681 taataaaaaa tcccgacgtc atgtcgggat ttttcggtat ggagcgcata ggtagggctg
     1741 actacctgat ggtcatggtg cctgactgat tattattgtt gttttaagtc attgaacatc
     1801 ctgtccatat agatcaatca taagagcttc atcgggggat agatacagac aatttcgtga
     1861 aacgctgtaa gccaaaacgt acaaaaaata atcaaaattt atcccaatgg ttatccgcag
     1921 aaattgtgga ttcttttggg taaaaaaggc ttaaaaactc tatggaattg ctatttttga
     1981 tcattattta accaatttta ttggcaagaa gtatcaaact tgctcaaaaa aaatccgctt
     2041 tgtttccaaa gcggattttt gcggtagttt tgggttatgt aaaatagcta agtacatgat
     2101 tttatttaac ttataaaaga cgatttcgga taacgcccat tatgttaaat agagataaga
     2161 catactctaa ttttagagtt ttcttttagg tatgatataa aaatatacaa gcctaacagt
     2221 ttagcttaaa ggttaaaaat tgaataaatt aacaaatatt tttatggctt taggagtttg
     2281 cctttcaatg gaaacatttg cagaacctgt tttttctaat gatttattag cgaaagcaga
     2341 aaatggagat acttctgctc agttagaatt agccgagatt tatctatatg gtcatggtgt
     2401 tgattcagat gaaaatcaag ctgaagtttg ggctcttaaa tcagcagaaa atggaaatgt
     2461 agcagcaatg ttttggttag ctgatggata tgttacctat gctagattaa tggaagatga
     2521 tgacaaaaac gattctttag aacatttcca aaaagctttt aagtggtttc agaaagcctc
     2581 agaaaatggc cattctgaat caatggttga gttagctgat ctatatactc gtgcagatag
     2641 cggaatagaa gttaatatta ataaagcttt tgaacttcgt gaaaaggctg caaagttagg
     2701 taataagaaa gcaatgcgaa gtctttccgt tatgtatcgt gatggtatag gtattcctaa
     2761 aaatactgat ttagctcaaa gttggtggga taagtctgaa aattagtttt aatatttcct
     2821 aaaattaaca taatacacct tatacgaagt caaaaaaata tatctatatg ataattaatg
     2881 aaattgtaat aactattcat aatgaccatt atgtaaaatg gatatgatag aagtgtacta
     2941 gtgagtacac ttctatcata aaaaagagag ctgctagctc tcttttttat gttttattca
     3001 aaatgaagtt gatatttatc cgtcgtaatc ttgatcttga ggttcttata ttttcgtttt
     3061 gtggcactca gggctgaatc tgtcacttct gcataaaatt tagggtcatt cattagatca
     3121 ttaaatagtt tatatccaat caataactta ctggattttt tcttttcctt tagatcattt
     3181 tcaatcaact gatccaattc ttttacagaa aaactatgca tattaccctc tatagtatct
     3241 ttctgataaa acaccataca gacttagcaa gaggatttaa agcttattag ataattgata
     3301 tttatgaaat tgtgagaaag ataatatatt agatttatct atttaagtta agtttttcta
     3361 aaaataacat aatacacctt atacgaaatg cgcaaaattt ttccttttaa aacagtgtct
     3421 tatatattta aaaacagcct aagcagtcac tcttaggctg ttttagcaat ttttcgtgtt
     3481 ccacgcttac tatttgagca atgttccacg gttttgttca gttttaataa acatcatccg
     3541 gttttttgat gagtcattat ttcatgtgct tgcactgaaa ttggctcatc aaaaaagtgg
     3601 agtcctggct aaccataagg aaggaaaaaa cggctcagga agcccctctt cttaggttca
     3661 ggctcaacgt gttctggaac agggatacgt ttgttctctg gagtagtcaa tccgtcatct
     3721 ttaggtggaa cgactattac ctcttctttc atttctacaa tgggagctga tttagggagt
     3781 tcgagttcac tagggtcttg gatctttggt ttgggtgctt ccagtagacg ttgcatcgtc
     3841 tcaatttgat tttgataaaa ggattcacgc tcttttgcat catcaagctg attttttagc
     3901 atgtctatct gttgcctaag tagtaaaact tctgccgaat ttggactgtc tatttttaca
     3961 gactctttta catctagttt ttttgaaggt tcgccaaaaa cacggatagc ttcagccaga
     4021 tcaattttat tatcagaatt tttacttaaa attcctttct ttatgttgtt gtaaatcgtt
     4081 tgtctattga tattgtaaag cttagaaagc tcaagaactg taaggctttt catgctgtaa
     4141 atctatgcct aactctgtca aaagttgttg tctattagac tgcctaatag acaaatgatc
     4201 aagtataaat tttaggcagc ttgatagcca actttttgaa gataagggaa taattcttgg
     4261 aatttttcac tatcttgaag catttcagca atacgtatag cgaattgtgg atagctttct
     4321 gtaccctgag aatacttacc catttctggt agttcggaaa gtttagtagc gaataagtgg
     4381 cgttgcttat ctgtcattct cgagaacaga tcagttgtat taggatttct attagtttct
     4441 atagatcgaa tagttgcttt ctttggctta aagctaaaag aaaatcctga gattgaacga
     4501 ccttttttat gttgttcgta tttaacagtg atatctgaaa attcgttgat ttgatctact
     4561 gcaatatcta aaacacgtct cttaaatgct tccattcgag tgtattcgtt aacccctata
     4621 ccaagttggc tacgaaattt atttaattca taaacaggcg ttttccctac agacttccat
     4681 tgcattaaga gttcataaag acgtactgca taaacgcttt tgaagtcagc agtttttttc
     4741 aaatgataac tagtaaagta ctgttcaaac ccatctattt tagtgacatg ttcaattacc
     4801 acacgagtta aggtaactaa aattcttcct tcacccttcc gataattagc atcttgaatc
     4861 cagcgacttt ttgttaaagt tccatcttca ttggtaatcg taaattgtct tttaaaaaga
     4921 gaatcttctg cttcaattaa cgctagataa gctgcatctg gagaaacgtt aaatgcttta
     4981 gcatatctaa gagcatttaa ttctaaaggt tcatctgttg ataatccttg tccagtctca
     5041 cgagcgtcta caattgcgag ctgaagtaaa cgggtttcgc ttaaagtaag tgtctgtaaa
     5101 gcttgtacaa gtttattaga ttttacgact aagtctgtca tgtcattcta tttagcaaca
     5161 atcttattgt gttgctataa caacataata aatatttgtt gtcaaatacc gaggaaacgt
     5221 tgctatataa ccgaggaaac gttgctatat aaccgaggaa acgttgctat ataaccgagg
     5281 aaacgttgct atataaagct tgaaagcctt ataaataaaa ggattcagtc gagttaaaaa
     5341 catttaaaaa ctttaaaaat aattaaaagc atatttcatg aaaaattgcc cttggttttc
     5401 gcttcgctca aactctattg aatctcgctt tcgctcgatt cgtcggggca atttttggtt
     5461 aatattttcg tgacttttag aaaagcaaaa gcaagatcaa aagcagctcg gaattcgctt
     5521 cgctcataag cttttttttc tcgctacgct cggtttaggg atcaaaattg gaaagttcgc
     5581 acccattcgg gatgctctgt aaagcaggtg attgatggat agggagcttg taatcaggga
     5641 aagggatggg caaattttct cagttcgctc gtagacactc gctctgttta gcgactcatg
     5701 ccacgagatt tcattctagg cgcatgttgc tgtgctttga gttgttgttc ttgtctgagt
     5761 ttcagctgtt gttctgcttt gatctcattc acacattttt taatgacttg ataggttgag
     5821 tatagagtct ggtattgctt ggctttggcc ggatgttgtt gttggtactg cttccaggca
     5881 tgttctttga acttttcatt ttccaataaa tccttcatac catgtttttt ctggtgttca
     5941 tgctgacctt ttagcttttt gtgttcctga taaacctcgt cacgttgggc ttcccaggct
     6001 ttttttccaa acagtaaggg tttgttctgg gttaattcat tgtgttgatc gaccagactt
     6061 tgcaacttgt catgactttg tttcagtctg tttttcacga tgtcttgggc gagctgctga
     6121 ctaaactcat ttttatgctt ttgcatcttg cccaaggtgt tttctgcctt tttaaaaaca
     6181 tcctgcataa attctaggtc tagattcgcg tcttttgcgc tgatttcgcg atttagcact
     6241 ttgccaaggg tttccctctg tttttcttca aacagggctt tttcgtcaat tggtggcgtt
     6301 ttagcactat ttttgatttg gatctgctgt tcagttttta gtgtattgag tttgatctca
     6361 ttttcagcaa tcagcagatc catatttttg ccgtattgca actgagcctg atttctctgt
     6421 ttgatttcgt cattgcttcg gctgatttcg gtctcaatcc cttttcggcg cagttcggtg
     6481 actttcggcc cttcgtgcag cgtggcttct aaaccattct cctggtcttt gtagctgcgg
     6541 tggtcgactc gggcagtgta tccagccaga tccaggtgca tattgcagat atcggcaaag
     6601 tgttcgcgcc agtgttcgat ttctccactg tgtttctgat ccagttcccg ggttttctcg
     6661 gtgaagcctt ccggagtaag tttgcgtgtg ctcatcagga tatgcgcgtg ataattgcgt
     6721 tcatcactac cagatccagt atgcggtgca tgaatacagg catcgacggc cacctgatgt
     6781 ctgtccacga tactggcgca tagctctttg agcatggcca ggcgttgttc ctgatttaat
     6841 tcacttggaa aagcgatttc aaattctcgg gcaactgtcg agttttttcg ggtttcccgt
     6901 tgttcaactt cgttccagag tctttcccgg ttttttaaat gttctggtgc gccttcgggt
     6961 aaataaattt ctttatattc cacacctgtt tttcggctgt aatcatgttc tcggccctca
     7021 cgttcacaat agatcttttc acctgcccga taggcaatcg cagcagtagc ggaacgccct
     7081 gctgatcgtg ctacggtttt gactgaaaag tggtaaatcg ccatgtctat ttttcgcttt
     7141 taaattttca cgtagtgaaa tacaaccgtg aatacggttg tcggggtatt gggggagacc
     7201 ccaatcggac ttgcaaactt cgtttgcata agtgcgctct tcgaggaaaa cattactata
     7261 catatagcac cttgaatgca tattcaagtg cgcttatgtt agggatggtt tcccaggatg
     7321 atggtattag attaatgagc gttactgaaa cactagacag caaaattaaa gcccaggaag
     7381 aaaagctaaa gcagttaaag gctcaaagac aggcagcact agcaagagaa cgtgctaaag
     7441 agaaagaaca ggctcgtaaa gatgatatta gacgcaaaat tctaatcgga tcttgtatgc
     7501 taaaaatcac tgaagaagat gaacaggccc gggcaaaatt gattgcacag atggacaagt
     7561 atttgactga tgaacgtgat cgtaagctct ttaatttata agtgatagga aatcaatcat
     7621 ggcattgagc caattcgata ttattaagtc tttaggtgaa tctattgaat ggtttgaaaa
     7681 agaactctct tggggaatgc ctattgcaga attaaggcat ttaacaggaa gaattgggga
     7741 actttatgta gctatgatga ccttaggaca attagctgat tcagttaatc agagaggtta
     7801 tgatgtagtt tccagtactg gacaaagaat ttctgttaag actgttacta cagcaagtca
     7861 tgtcgatttt aatcttaaaa cgattgagtt agtggacaga attattgttt taagaattaa
     7921 cccagaggac ctctgtattg aagtagtcta tgacttaaat atggagcagt ttcaggctaa
     7981 gtcacgagaa tacaaaggga aatatatttt aggtatttat aacaaaatac ttttagctaa
     8041 ttcaaaaata aatatagggt taaatattcc tactgaaaca gaactgaaag ttgaaaataa
     8101 tcatgagcta ttgattttaa ataatatttt ttatgatgga tatgagataa aacaatatga
     8161 aaatgcaaaa attgaagttt ataaggatgg tgaaaaacaa ccatcagcta aagaaatttt
     8221 gaggcagata gccctccctt taaatgtctc tttaaataat tcagccggaa atcctaaaaa
     8281 tactcagcaa ttaggggatc atgtgatcaa ggcaataaag aactttagat caacctgatt
     8341 tcgcataaga gattttatgt taaagaaaat attaaataat ataataaata acttattgaa
     8401 tttaattaat ttttaaagaa aaaaatatgg attttataag aaaaattaat atttatatca
     8461 aaaaacttga ttatttttat ttttactgta tatttaactt aactagaaaa aaagtcttgt
     8521 ttcggtaaat cttccttaag tatcgcagtt ttagtcataa tccttcgttt t
//
