LOCUS       NZ_CP087577             6647 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Escherichia coli strain OSUCMP42NDM plasmid pCMP42-3, complete
            sequence.
ACCESSION   NZ_CP087577
VERSION     NZ_CP087577.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23219802
            Assembly: GCF_020985225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Ballasha,G.A.
  TITLE     White-tailed deer from urban and suburban environments serve as
            reservoirs for Cephalosporin and Carbapenem-Resistant
            Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2020
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 865x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 02:12:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,931
            CDSs (total)                      :: 4,814
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 74 of 205
            Pseudo Genes (incomplete)         :: 127 of 205
            Pseudo Genes (internal stop)      :: 35 of 205
            Pseudo Genes (multiple problems)  :: 32 of 205
            Pseudo Genes (short protein)      :: 3 of 205
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087577.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OSUCMP42NDM"
                     /isolation_source="Cecal"
                     /host="Free-range White-tailed deer"
                     /db_xref="taxon:562"
                     /plasmid="pCMP42-3"
                     /geo_loc_name="USA: Ohio"
                     /collection_date="2019"
                     /collected_by="Ohio State Univ"
     gene            join(6419..6647,1..191)
                     /locus_tag="KUA31_RS00410"
                     /old_locus_tag="KUA31_00410"
     CDS             join(6419..6647,1..191)
                     /locus_tag="KUA31_RS00410"
                     /old_locus_tag="KUA31_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(509..742)
                     /locus_tag="KUA31_RS00415"
                     /old_locus_tag="KUA31_00415"
     CDS             complement(509..742)
                     /locus_tag="KUA31_RS00415"
                     /old_locus_tag="KUA31_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(749..1267)
                     /locus_tag="KUA31_RS00420"
                     /old_locus_tag="KUA31_00420"
     CDS             complement(749..1267)
                     /locus_tag="KUA31_RS00420"
                     /old_locus_tag="KUA31_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<1305..2003)
                     /locus_tag="KUA31_RS24550"
                     /pseudo=""
     CDS             complement(<1305..2003)
                     /locus_tag="KUA31_RS24550"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1945..2268)
                     /locus_tag="KUA31_RS00430"
                     /old_locus_tag="KUA31_00430"
     CDS             complement(1945..2268)
                     /locus_tag="KUA31_RS00430"
                     /old_locus_tag="KUA31_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2332..2523
                     /locus_tag="KUA31_RS00435"
                     /old_locus_tag="KUA31_00435"
     CDS             2332..2523
                     /locus_tag="KUA31_RS00435"
                     /old_locus_tag="KUA31_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_169774213.1"
                     /translation="MTKQEKTALNMVRFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3193..3351
                     /locus_tag="KUA31_RS24555"
     CDS             3193..3351
                     /locus_tag="KUA31_RS24555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            3849..4076
                     /locus_tag="KUA31_RS00440"
                     /old_locus_tag="KUA31_00440"
     CDS             3849..4076
                     /locus_tag="KUA31_RS00440"
                     /old_locus_tag="KUA31_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(4073..5641)
                     /gene="cea"
                     /locus_tag="KUA31_RS00445"
                     /old_locus_tag="KUA31_00445"
     CDS             complement(4073..5641)
                     /gene="cea"
                     /locus_tag="KUA31_RS00445"
                     /old_locus_tag="KUA31_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            5901..6338
                     /locus_tag="KUA31_RS00450"
                     /old_locus_tag="KUA31_00450"
     CDS             5901..6338
                     /locus_tag="KUA31_RS00450"
                     /old_locus_tag="KUA31_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     Typing          complement(2609..2696)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2707..3265
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.544"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(406..1955)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gaaaatcacg gagtgatgtg caacaacgca ttcagcagtt tcacagccag gaatttttaa
       61 actcactccg gggcacaact caatttgcgg gtactgatta ccgcagcaaa gaccttaccc
      121 cgaaaaaatc caggctgctg gctgacacga tttctgcggt ttatctcgat ggctacgagg
      181 gcagacagta agtggattta ccataatccc ttaattgtac gcaccgctaa aacgcgttca
      241 gcgcgatcac ggcagcagac aggtaaaaat ggcaacaaac cacccgaaaa actgccgcga
      301 tcgcgcctga taaattttaa ccgtatgaat acctatgcaa ccagagggta caggccacat
      361 tacccccact taatccactg aagctgccat ttttcatggt ttcaccatcc cagcgaaggg
      421 ccatccagcg tgcgttcctg tatttccggc tgacgctccc gttccaggga taacacatgt
      481 tcgcgctcct gtatcagccg ttcctctctt atctccagtt ctcgctgtat aactggctca
      541 agcgttctgt ctgctcgctc aagtgctgca cctgctgact caactgcatg acccgctcgt
      601 tcagcatcgc gttgtcccgt tgcgtaagcg aaaacatctt ctgcaattcc acgaaggcgc
      661 tctcccattc gctcagccgc tgcatatagt cctgttgcag ctgctctaag gcgttcagca
      721 aatgtgtttc cagctctgtc actctgtgtc actccttcag atgtacccac tctttcccct
      781 gaaagggaat cacctccgct gatttcccgt acggaaggac aaggaatttc ctgttcccgt
      841 cctgcacaaa ctccacgccc catgtcttcg cgttcagttt ctgcaatgtc tcttcctgct
      901 tcctgatttc ttccaggttc gcctgtatcc tccctccaag ataccagagc gtcccgccac
      961 tcgcggtaaa caggagaaag actatcccca gtaacatcat gcccgtattc cctgccagct
     1021 ttaacacgtc cctcctgtgc tgcatcatcg cctctttcac cccttcccgg tgtttttcca
     1081 gcgattcctc tgtcgaggct gtgaacaggg ctatagcgtc tctgattttc gtctcgtttg
     1141 atgtcacagc ctcgcttaca gattcgccga gcctcctgaa ctcgttgttc agcattttct
     1201 ctgtagattc ggctctctct ttcagctttt tctcgaactc cgcgcccgtc tgcaaaagat
     1261 tgctcataaa atgctccttt cagcctgata ttcttcccgc cgttcggatc tgcaatgctg
     1321 atactgcttc gcgtcaccct gaccacttcc agccccgcct cagtgagcgc ctgaatcaca
     1381 tcctgacggc cttttatctc tccggcatgg taaagtgcat ctatacctcg cgtgacgccc
     1441 tcagcaagcg cctgtttcgt ttcaggcagg ttatcaggga gtgtcagcgt cctgcggttc
     1501 tccggggcgt tcgggtcatg cagcccgtaa tggtgattta ccagcgtctg ccaggcatca
     1561 attctaggcc tgtctgcgcg gtcgtagtac ggctggaggc gttttccggt ctgtagctcc
     1621 atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc cacccacagg
     1681 atgctgtact gatttttttc gagaccgggc atcagtacac gctcaaagct cgccatcact
     1741 ttttcacgtc ctcccggcgg cagctccttc tccgcgaacg acagaacacc ggacgtgtat
     1801 ttcttcgcaa atggcgtggc atcgatgagt tcccggactt cttccggatt accctgaagc
     1861 accgttgcgc cttcgcggtt acgctccctc cccagcaggt aatcaaccgg accactgcca
     1921 ccaccttttc ccctggcatg aaatttaact atcatcccgc gccccctgtt ccctgacagc
     1981 cagacgcagc cggcgcagct catccccgat ggccatcagt gcggccacca cctgaacccg
     2041 gtcaccggaa gaccactgcc cgctgttcac cttacgggct gtctgattca ggttatttcc
     2101 gatggcggcc agctgacgca gtaacggcgg tgccagtgtc ggcagttttc cggaacgggc
     2161 aaccggctcc cccaggcaga cccgccgcat ccataccgcc agttgtttac cctcacagcg
     2221 ttcaagtaac cgggcatgtt catcatcagt aacccgtatt gtgagcatcc tctcgcgttt
     2281 catcggtatc attaccccat gaacagaaat cccccttaca cggaggcatc agtgactaaa
     2341 caggaaaaaa ccgcccttaa catggtccgc tttatcagaa gccagacatt aacgctgctg
     2401 gagaagctca acgaactgga cgcagatgaa caggccgata tttgtgaatc gcttcacgac
     2461 cacgccgatg agctttaccg cagctgcctc gcacgtttcg gggatgacgg tgaaaacctc
     2521 tgacacatgc agctcccgga gacggtcaca gcttgtctgt gagcggatgc cgggagctga
     2581 caagcccgtc agggcgcgtc agcaggtttt agcgggtgtc ggggcgcagc cctgacccag
     2641 tcacgtagcg atagcggagt gtatactggc ttaaccatgc ggcatcagtg cggattgtat
     2701 gaaaagtacg ccatgccggg tgtgaaatgc cgcacagatg cgtaaggaga aaatgcacgt
     2761 ccaggcgctt ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc
     2821 gagcggtact gactcacaca aaaacggtaa cacagttatc cacagaatca ggggataagg
     2881 ccggaaagaa catgtgagca aaagaccagg aacaggaaga aggccacgta gcaggcgttt
     2941 ttccataggc tccgcccccc tgacgagcat cacaaaaata gacgctcaag tcagaggtgg
     3001 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3061 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3121 gtggcgcttt ctcatagctc acgctgttgg tatctcagtt cggtgtaggt cgttcgctcc
     3181 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3241 tatcgtcttg agtccaaccc ggtaaggcac gccttaacgc cactggcagc agccactggt
     3301 aaccggatta gcagagcgat gatggcacaa acggtgctac agagttcttg aagtagtggc
     3361 ccgactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3421 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgtt ggtagcggtg
     3481 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3541 taatcttttc tactgaaccg cgatccccgt cagtttagaa gaggaggatg gtgcgatggt
     3601 ccctccctga acatcacgta tatagttagc ctgacatcca acaaggaggt ttatcgcgaa
     3661 tattcccaca aaaaatcttt tcctcataac tcgatcctta taaaatgaaa agaatatatg
     3721 gcgaggttta atttatgagc ttaagatact acataaaaaa tattttattt ggcctgtact
     3781 gcacacttat atatatatac cttataacaa aaaacagcga agggtattat ttccttgtgt
     3841 cagataagat gctatatgca atagtgataa gcactattct atgtccatat tcaaaatatg
     3901 ctattgaata catagctttt aacttcataa agaaagattt tttcgaaaga agaaaaaacc
     3961 taaataacgc ccccgtagca aaattaaacc tatttatgct atataatcta ctttgtttgg
     4021 tcctagcaat cccatttgga ttgctaggac tttttatatc aataaagaat aattaaatcc
     4081 ctaacacctc atttatagta ttaagtttat tcttatcaat ataggagcat agaattcctg
     4141 taacaatagc aataccccaa atacctaatg tagttccagc aagcaagcta aaaagtaaag
     4201 caacaacata actcacccct gcatctgcag ctttcttctc taatgtaaga aatagtggct
     4261 tccagtcacc tgtatcctta atttttagga tatcagatac cacatcatat ccaaaagaaa
     4321 catgccccgt aatcttcaag tacttggcaa actgatctaa atgtttagcc cagtcatcat
     4381 acttcaccga tgccaacgca ttaaaaatag catcacgatc ggctttgctg aatttcttat
     4441 ttaaaacatc cttgtatttt tcaaaagcag cgagagcttc attcacattg ccgattttct
     4501 tacctttaga cttatcagca agttcctgtg ccattttcga atatttttca ccatattttt
     4561 cagtcagcgt ttgataaaag ctaactgttg catcaacagc atccttaatc tgtgaattaa
     4621 ggagattatt ctgtgctttt ttcaaatttt cttcagcttc atgaacacga gcgataccgg
     4681 cattacgatt attactgacc tgagaaatag ccttctggat ctgagttata tcagcattta
     4741 tccggttaat acgtgtttct gatgctgtta cctgtttttg tttttcttct ctaatcttac
     4801 cggccccaac ccgtcgtctg gttgcttcaa aaaaaggacg gttctgaagc ggatcattgg
     4861 ctcttggtga tagttttttg accagctcat ccagttcttt atatttagcg gatgcctgag
     4921 ccagttcatt tcgttttcca gcgagcgttt tcatttctgc atcacgggca tggatactgg
     4981 agcttaaacg agaattgaga gtcttaatct ctccatccat tttcaccact tcagattgtg
     5041 cagcagaaag ttttttttgg gcgatctcaa cagctttagc ttcttcactc aatgcagcca
     5101 gtcgtttctc ttcagcttca gccagtttca actggcgttc tgtttcagcc ttctcccgtt
     5161 caatctcttt acgtcgttgt tctgcttcct gaaaagcctt ttctgctgct tccgcttctt
     5221 tacgggcttt ttcttctgct ttcgcaaggc gcaaacgctc tgcttccgcc tgcatagctg
     5281 cattattagc atgagcaagc tctgttgctg aaggcgtacg tgaggcattg tgacgaagag
     5341 cctcattcac gatatccttc aggcgctgag tcagcgcatc cctgtttgcc tttgctttcg
     5401 cctgtgcttc cgctgcagct tttgcccggg cagcctgctc tgcctgtgtt ttctttaatt
     5461 gagcagtaga ccatttagca gttgcatgaa tagctgcaga actttcactt ttactgcctc
     5521 cttttccacc tccgccgcca gagccactcc cgtcaggagt accattcaaa agagtaataa
     5581 ttacctgtcc cttatcatca taaggaacac catctttata gtacgctacc gcggtttcca
     5641 ttataaaatc ctctttgact tttaaaacaa taagttaaaa ataaatactg tacatataac
     5701 cactggtttt atatacagca taaaagctac gccgctgcat tttacctgtc aagactgtgg
     5761 acttccattt ttgtgaaaac gatcaaaaaa acagtctttc acaccacgcg ctattctcgc
     5821 ccgatgccac aaaaaccagc acaaacatta ccgttctcag acctcattat gttttactga
     5881 aactatgaga tgagacatct atgggacact gtcactttat ggcatggcac acactccggg
     5941 acgcactaaa aatgacaggc agatcgcgtt cacagtttta ccgtgatatg cgcgcaggcc
     6001 ttgtcagtta ccgtaccggc agggacggac gacgggagtt tgaaaccagt gaactgatcc
     6061 gggcatacgg cgaattaaag cagaatgaga caccagaaag gcacagtgag ggacatgcag
     6121 aaaatccaca tgatcagcag acagaacgca ttctccggga actgaatgag ctgaaacaat
     6181 gcctgacgct gatgcttgag gataaacagg cacaggatat ggatcgcaga cgccaggaag
     6241 cagaacggga acagctacaa aatgagatag cccagctcag gcaggcactg gaactggaaa
     6301 agaaacgggg attctggtcc aggttgttcg gtcgctgaac gctgtcagag actgatgata
     6361 aaatagtctt cggataataa ctcaccgaga ataaatactt taaggtaggg agacactcat
     6421 gagacgtacc ggaaacaaac tttgtcttat cgccatgata acagcaacag tagctctcac
     6481 agcctgtacc ccaaagggca gcgtggaaca acatacccgg cattacgtat atgcttctga
     6541 tgacggtttt gatcccaact tttccaccca aaaagccgac acaacacgaa tgatggtgcc
     6601 tttttttcgg cagttctggg atatgggagc taaagataaa gcgacag
//
