LOCUS       NZ_CP087574             4072 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Escherichia coli strain OSUCMP42NDM plasmid pCMP42-5, complete
            sequence.
ACCESSION   NZ_CP087574
VERSION     NZ_CP087574.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23219802
            Assembly: GCF_020985225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ballasha,G.A.
  TITLE     White-tailed deer from urban and suburban environments serve as
            reservoirs for Cephalosporin and Carbapenem-Resistant
            Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2020
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 865x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 02:12:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,931
            CDSs (total)                      :: 4,814
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 74 of 205
            Pseudo Genes (incomplete)         :: 127 of 205
            Pseudo Genes (internal stop)      :: 35 of 205
            Pseudo Genes (multiple problems)  :: 32 of 205
            Pseudo Genes (short protein)      :: 3 of 205
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087574.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OSUCMP42NDM"
                     /isolation_source="Cecal"
                     /host="Free-range White-tailed deer"
                     /db_xref="taxon:562"
                     /plasmid="pCMP42-5"
                     /geo_loc_name="USA: Ohio"
                     /collection_date="2019"
                     /collected_by="Ohio State Univ"
     gene            complement(join(2692..4072,1..767))
                     /gene="mobQ"
                     /locus_tag="KUA31_RS00020"
                     /old_locus_tag="KUA31_00020"
     CDS             complement(join(2692..4072,1..767))
                     /gene="mobQ"
                     /locus_tag="KUA31_RS00020"
                     /old_locus_tag="KUA31_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_057077964.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRENRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMEFHLKTREVPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            946..1200
                     /gene="mobC"
                     /locus_tag="KUA31_RS00025"
                     /old_locus_tag="KUA31_00025"
     CDS             946..1200
                     /gene="mobC"
                     /locus_tag="KUA31_RS00025"
                     /old_locus_tag="KUA31_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     gene            1733..2434
                     /locus_tag="KUA31_RS00030"
                     /old_locus_tag="KUA31_00030"
     CDS             1733..2434
                     /locus_tag="KUA31_RS00030"
                     /old_locus_tag="KUA31_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     Typing          complement(410..768)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          769..945
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.438"
                     /coverage="100.0"
                     /evalue="2.610000000000001e-86"
     Typing          1734..2434
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.715"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4..854)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.887"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(2696..4071)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.896"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 agaaggtcag ccacacggcg ggcaggctct gtcagccctc cgttcctgag ttcggtgcgg
       61 gaaatctgta tttccaggtt aagcctgtta atcagacgat tctgttcgcc gcgttctgtt
      121 tcgataccgc gtgtttccat cgcacaggca gactttccga ggtgaatacc aggcgttttc
      181 tcaagcccct gtcgctcgta tgaacgatgg tctatctctt cctgataacc ggcgttcgcc
      241 agtgccctgt ttgcatggtc agcccatgat gcccgccacg tctcggcatg tgtccggtcg
      301 ttccagtccc tgacctttcc gctgaaccca gcaggtccga cagccctcgt ggtcagcatg
      361 atgtgcgcat ggggatttgt tttatccatg tgatggaacg ccacatcagc aatcatgccc
      421 tgactgacaa agttttctct gacaaaggcg agaacggttt cccgtgcggc atcctgcggc
      481 agttcgtggg ggatggcgag ttcaatttcc cgcgcaagtt gtgagttctt ccggcgttcg
      541 gccttctcaa cggcattcca gagttcggca cggttcgtac accaggcagg ggcggtggcg
      601 ggggtcagaa tcacagcctc agccacaccg cttttgcggg tgtagtcgtg agtcagtccg
      661 gtacgctcat caagcatcag tgaaccggaa cgataggcag cggaagctac ggcgctgtag
      721 ccgctgcttc tggagataat tttcatgctg agatgataaa tcgccatttc tgccgtaatg
      781 cctttttgta gttttctttc agccccgcag ggcgcacaca atcgaaacgg agtggagatt
      841 gtataagtgc gcacttcgtg atttgccgtg cattatacca gctcagtttt taaactacca
      901 gtgacgcgct aaaatgcgat ttttcatcgc gcacggagtt acatcatgag cagaacgctc
      961 gaacagaaga ttgctgatgc tgaggcgaga ttacagcgcc tgaaggcgaa gagccggagc
     1021 ctggacacag cgcagaaggt tgttgtgggt gctgcattgc tggcaaaggt cagaaagccg
     1081 gaggaggtgc agttgcgtgc ctggttactc cagttcctga aggcagaggt gacacgacag
     1141 gctgatgtga cgcgcatact gccgctgatt aacgagctgg aggcgctgcc agaacagtga
     1201 ttgtatcgtc cgctcgccgc agccccagcg accgagcgtt agcgagcgag tgggcgagga
     1261 agcggaagtg aatcagtcgc caggttgagc acatacgcac ggtttttcgg caggtgatat
     1321 gcgctgaacc cgcatgaata ctgggtttcc acagggttgg agggggtgta ttcgaaactt
     1381 gcaattctgc tttattgcaa cgcattgttt ttattggttt tttgttttta gcaccccaag
     1441 ttatccacag gctcgccgtt aagtttttaa gatcttattt ttagatcttt tttttaaaac
     1501 ctttttaggt cttgtaacac catgatttta aagatgttct ggcaagataa gtggagcttt
     1561 tgggggtgaa aggtggagct tttgggggtg aaaggtggag cttttggggg tgaaaggtgg
     1621 agcttttggg ggtgtaatcc acagaaaaag ggggttgcaa aaaaaaaact tccacccata
     1681 tgataagtgg cgtacagtaa aaaaacgcca ccaatggcaa cgagcaagcg aaatgtcaga
     1741 tctgatagct tacaaatcaa acgcccttat cgaggcaagc tacaagctca ctttgcagga
     1801 acagcgcttc ctgctggtct gtatcagccg cctgaaatcc ggagaagatt ccccttcgcc
     1861 tgatgaacaa aaaaccatga ccatcacggc tgcagagttt catgatgcct ttcctgacat
     1921 gggacggcag cacgctgaag ctaggctcaa ggaagcgata gatcgcctct gggacaggtc
     1981 agtaatcatc aagaacgagg aaaaacgaga agagttccgc tgggtgcagt accgggcgca
     2041 gtatttcaaa ggtgaggcga aagtagatat caccttctcc gatgccatca tgccgtatct
     2101 gacacagctc aaggggcagt ttacgcgggt agtcgtgaag aacgtctcca gcctttccag
     2161 cacctacagc atccgcattt acgagctgct ccagcagttc cgcagcaccg gtgacagaac
     2221 gatagctatc gatgatttcc gaaccatgct tgccgttgaa gataaatatc aacaattcaa
     2281 agacttgaat aagatgataa tcaagccagc catcgccgaa ctgaacgaaa aaagcgatct
     2341 ggttgtaact gttgagacga tcaaaaaggg gcgcacggtt gtagccctgc attttcgctt
     2401 caaggaagac aaacagatca agatgacgat ctaaaatcga ttttaaggca cctaaaacgc
     2461 tccctgctgc gttttttttg tgttagtagt gcgaaggtac tattgaagtg aaaaattgcg
     2521 ccaaaaacgc tctgatgcag cgaaaaaaga acccccaatt tttgggggcg gaaaaacaaa
     2581 actatgcttg cgttatgtga gtgtgcttct tgcattcatc cctgtcgatg aacatcgaag
     2641 aacatcaacg aacataacac gatgttaaac aaaacaaaat attgttcaca ttcactgtga
     2701 ccaaccgcga tcatgtctcc tctcggtggt gtgtgttctt gcatcttcct cgcgccgcca
     2761 ttcgtcccag gtacgattga aacccagcga gcggaacaga tgatccggct gtttttctgg
     2821 tgtgttgacg gcagcaaaaa gcggtacctc acgggttttc agatgaaact ccataagttc
     2881 cggcgcaggt gatatgtggc tgtctgcatc gtgatttacg gcaacaccat accctgcata
     2941 ccgtaacccc ttatcggtca gtgcactgat gaccagctgg ctgttatcgg ctgagggaac
     3001 ggcaaacgcg ggatacagcg taccattaag ccggacaaca ccgcccagag agtcatccac
     3061 gcccatgacc agcaaaagct gaagctggcg gttttcccgg tcgacagaac tcatgaccgg
     3121 catcagcgtc ggtggcagac gtccctgcat aaaatccacc ttccttgccg ctggtgatgg
     3181 catggctggc tggcggtctt ctccaagaca gaaagactcc ggccatgtct tcggctggcg
     3241 gagattgcgg gtatgttgcg gccatgtctg gcaaacctgc gtcagcagtt gctcaaaggt
     3301 tgcagaggaa taaacacgtt gcagtggcgg acgttggccg atttcgcttc ggtcacagat
     3361 ataaccactc ctgtcacgca ttaaatcaac actctcaaag attctggtgc ggtatttctc
     3421 cagctcgttc ccccagagcg tatattctcc gttccggttg tgaagcagaa ggtaacaggt
     3481 catttccccg ttaccaggca tgggagcctc gccatacccc gtaacgactg ctctcagcac
     3541 ccctgaatcc ataacggcat tgtcattttt cctgagctgc tccgcgcgac tacgttcagt
     3601 atcttcctgt tccttccggc gcaattcctc ttcacgttta agcaactggg ctctgagagc
     3661 tgcaaggccg gattcccccc actgctgcca ctgtttttca aatgccatct gactcgcggg
     3721 aatgtcctgt ttctccacct tccggtaatg ctgtcgtaat ttttccattt gctggctcag
     3781 agactggatt tttccctgct cactacggta ttccggtgcc gcccattgca tcaggggaat
     3841 acgggaaaaa ccggacgaaa acggcgatga ttttttcagg ctggcagcat ggctcatcgc
     3901 ttcctttcgt tcggtgttca gctcttccca gcgtctgcgc gtcgcctgca tatctgccat
     3961 catcgacatg gcattactgg tgagtttcca tgccgagtta ctgtccttcg gcaacgcttc
     4021 gataatgtca cgtagtgtgt acgaagttgc gttgtcagga agcccgatat tc
//
