LOCUS       NZ_CP087573             3230 bp    DNA     circular CON 23-JAN-2024
DEFINITION  Escherichia coli strain OSUCMP42NDM plasmid pCMP42-6, complete
            sequence.
ACCESSION   NZ_CP087573
VERSION     NZ_CP087573.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23219802
            Assembly: GCF_020985225.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Ballasha,G.A.
  TITLE     White-tailed deer from urban and suburban environments serve as
            reservoirs for Cephalosporin and Carbapenem-Resistant
            Enterobacteriaceae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Li,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2021) CVM/OR, FDA-CVM, 8401 Muirkirk Rd, MOD2,
            Laurel, MD 20708, USA
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: MAY-2020
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 865x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/23/2024 02:12:25
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,931
            CDSs (total)                      :: 4,814
            Genes (coding)                    :: 4,609
            CDSs (with protein)               :: 4,609
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 205
            CDSs (without protein)            :: 205
            Pseudo Genes (ambiguous residues) :: 0 of 205
            Pseudo Genes (frameshifted)       :: 74 of 205
            Pseudo Genes (incomplete)         :: 127 of 205
            Pseudo Genes (internal stop)      :: 35 of 205
            Pseudo Genes (multiple problems)  :: 32 of 205
            Pseudo Genes (short protein)      :: 3 of 205
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087573.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="OSUCMP42NDM"
                     /isolation_source="Cecal"
                     /host="Free-range White-tailed deer"
                     /db_xref="taxon:562"
                     /plasmid="pCMP42-6"
                     /geo_loc_name="USA: Ohio"
                     /collection_date="2019"
                     /collected_by="Ohio State Univ"
     gene            complement(944..1135)
                     /locus_tag="KUA31_RS00005"
                     /old_locus_tag="KUA31_00005"
     CDS             complement(944..1135)
                     /locus_tag="KUA31_RS00005"
                     /old_locus_tag="KUA31_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001041614.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDRLNELDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1539..1676
                     /locus_tag="KUA31_RS00010"
                     /old_locus_tag="KUA31_00010"
                     /pseudo=""
     CDS             1539..1676
                     /locus_tag="KUA31_RS00010"
                     /old_locus_tag="KUA31_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(2061..3146)
                     /locus_tag="KUA31_RS00015"
                     /old_locus_tag="KUA31_00015"
     CDS             complement(2061..3146)
                     /locus_tag="KUA31_RS00015"
                     /old_locus_tag="KUA31_00015"
                     /inference="COORDINATES: protein motif:HMM:NF026247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsG family protein"
                     /protein_id="WP_230282967.1"
                     /translation="MIYAFAGFFTGFYGLSVFCVFIIAVLCREFRLPNFALLCSIFIII
                     VLSFLYGFRSIDSGTDTVIYHAWFDSISKGSTNREFEPFFVLFGHFVNLFTNDAFIFIF
                     IVSIIQLLYVFKALLSLSGIIVVPLSLVISVSFLSGVDLISNTIRNGLSIAISSYAVIR
                     YLGGAGIVKYLIIIMISSMMHSSCIIFMIIPVVRRFLNEKYALRVFYTYVFIYLLSFLN
                     VFEFLFYSLNNIGVGALHVVDRIIAFKVQDIDMLGGSMKYYFFVITLIPFLLIKFGFYV
                     NKQLFTIHYVLLIPYAIIFLSPSSYRFSYISFYICVFIIAQAVYDSKSLLNRLLIYFAI
                     LAMILLTYTTNTMVNYKHVIY"
                     /name="EpsG family protein"
     Typing          1011..1622
                     /name="rep_cluster_2327"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.349"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaaactca ctccttgaaa cttaactaaa gaaccttgca aaagataaca cattagaaaa
       61 aaaaatagaa gttttaccga aaccaagcca gaggtgatta tttctcacgt actttctaca
      121 aattcagata tgcaatactt ttatttacca taacaccaca ttacgcgcac cgctcagacg
      181 gcctgagcgc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
      241 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
      301 cggaaattca cgccattcag ccggggtaag tcctgtttcc gatgttcttc ttacccgaaa
      361 acggtgaacg gggacgcaat ttattgcgtg gccgtgaggc gttgccggca cagccggaaa
      421 aaccattttc agcacgtgca ggaggcccag gaagaggggg cggatgtacg aatagctcag
      481 aggtcagatc tgacgtaaaa atgatgtaaa agtgatgtac ttttgatgta attctgttgt
      541 acacccgcga tctgacgtaa aagtgatgta attttgatgt acttttgatg taattctgtt
      601 gtacacccgc gttctggggt aaaagcagat gcaattttgt agtctatgtg tagtcgctga
      661 acccataggg tcatacgagc atgacccttt cccggaaaag gacgcttttt agccagaatc
      721 atacttaacc tgcggcgcca gcactcggga gtggtcgttt tatatactaa aaaatgacat
      781 acacctaacg cagctgcgat tttcatcgca acttcatgaa aagtaaataa aaaataaaaa
      841 acacagggat tctgggtatt accggggtaa taatgttaag cgctaacaca atgaaaatta
      901 aggcgatgcc ggtattactc cggtaatacc cagaacggag ccattacgca ccgtcctgct
      961 gctcattcag gcgcgctgac aggcttcggt aaaggcgttc cgcatcctca tggagtttct
     1021 cgcagaaatc agcctcatga tccagatcca gctcattaag cctgtccaga agaagcaggg
     1081 actgatcctg gatgaattta gccatgttaa cggcagtctt ttgttgtttg ttcatgaaag
     1141 ccccggcgcg aaagtgctta ttgtacgttc tgatggtttt ccgcttcctc gctcattcgc
     1201 tcactccgtt cggtcatgag tctgcggcga gcggagggag gataagcaga taacgtaacg
     1261 gtaagcataa cgaaagctaa ggacaagggc gaaaaggcca cgccccgccg gggcgttttt
     1321 ccataggctc cgcccccctg acaagcatca cagaatctga cgctcaaatc agtggtggcg
     1381 aaacccgaca ggactataaa gataccaggc gtttccccct gatggctccc tcgtgcgctc
     1441 tcctgttccc gtcttgcggt gaccgtgttg tggtggaagc tttacctaca tcaccacgtc
     1501 ccgtttccgt ttagacagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
     1561 ccgactacca cgccttgtaa ggtaactagc aacttgagtc caacccttac agacacgact
     1621 tatcgccagt ggcggtagcc attggtaact ggaactttag agggattgag tcttgaagtt
     1681 atgcgccagt taaggttaaa ctgaaagaac aagttttggt gactgcgccc tctaatagcc
     1741 agttacctcg gtttaaagag ccgcaaactc tacaaacctt caaaaaaacc gccccgcaaa
     1801 ggcggttttt ttcgtattgg agaaagagat tacgacgtaa aaggaaggat ctcaagtaga
     1861 tcatcttcct ggcaggtgta attcatgctg cagccggcca cccaaggcac aacttcaaac
     1921 ccgataaaag actccgaagg tactaaagtg tagccatagt gagttagcca aagacatggt
     1981 aaccatagtt caatggccag cactagcatg ctttgccttc gatgcagcta cagagaaact
     2041 atttatgtca gatatattta ttaatatata acatgtttat agttaaccat tgtattcgta
     2101 gtgtatgtta acaaaatcat tgccaatatt gcaaagtata ttaacaatcg atttaaaagt
     2161 gattttgaat cataaacagc ttgagcgata ataaacacac atatataaaa tgaaatgtaa
     2221 ctaaatcgat atgaagatgg cgataaaaaa ataatcgcat atggaattaa aagaacataa
     2281 tgaatggtaa aaagttgttt atttacataa aaaccgaact ttataagtag aaatggtatt
     2341 aacgtgataa caaagaaata gtatttcata ctaccaccaa gcatatcaat atcttgaacc
     2401 ttgaatgcga taatcctatc tactacgtgt aaagcaccaa caccaatatt attcaaagaa
     2461 tagaaaagaa attcaaaaac attcagaaaa ctcaggagat atataaacac atatgtataa
     2521 aaaacgcgaa gcgcatactt ttcatttaaa aaccgcctga ccactggaat tatcataaat
     2581 ataatacacg acgagtgcat catagatgaa atcataatga taattaaata tttaacaatc
     2641 ccagcgccac ctaagtatct aattactgca tatgatgaaa tcgcaataga aagaccattc
     2701 cttatagtgt tcgatattag atcgacacca ctaagaaatg acactgaaat tactaaagaa
     2761 agaggaacaa caataatccc tgacaaggag agcaaagcct tgaatacata taacagctgt
     2821 attattgaaa caatgaatat aaatatgaat gcatcattag taaataagtt aacaaaatga
     2881 ccaaataaaa caaaaaatgg ctcaaactct ctatttgtag accccttact tatagagtca
     2941 aaccacgcat gatatattac agtatcagtc cctgaatcaa tacttctgaa tccatataaa
     3001 aaagatagta ctattataat gaaaatagaa caaagcaaag caaaatttgg taatcgaaac
     3061 tctctacata aaacagcgat tataaaaaca caaaacacag ataagccata aaaacctgta
     3121 aaaaaaccag caaaagcata gatcatttat gtgcactttt aatatttaaa ataaacccat
     3181 gtaatttcca cattgcaagc ttaataaaaa acaagcaacc caatcaatta
//
