LOCUS       NZ_CP087324            14741 bp    DNA     linear   CON 12-SEP-2023
DEFINITION  Acinetobacter baumannii strain OC043 plasmid p3OC043.
ACCESSION   NZ_CP087324
VERSION     NZ_CP087324.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23078457
            Assembly: GCF_024749545.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Mellmann,A.
  TITLE     Sequencing of Acinetobacter baumannii genomes to construct genome
            database for SNP detection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14741)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 217x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/12/2023 05:08:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,904
            CDSs (total)                      :: 3,809
            Genes (coding)                    :: 3,700
            CDSs (with protein)               :: 3,700
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 30 of 109
            Pseudo Genes (incomplete)         :: 76 of 109
            Pseudo Genes (internal stop)      :: 13 of 109
            Pseudo Genes (multiple problems)  :: 10 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087324.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OC043"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p3OC043"
                     /geo_loc_name="Germany"
     gene            <1..123
                     /locus_tag="LO002_RS19430"
                     /pseudo=""
     CDS             <1..123
                     /locus_tag="LO002_RS19430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005194067.1"
                     /note="frameshifted; incomplete; too short partial abutting
                     assembly gap; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            116..418
                     /locus_tag="LO002_RS19435"
     CDS             116..418
                     /locus_tag="LO002_RS19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_001140622.1"
                     /translation="MAKTLQELLASRSPESQARIQQMADELLLETQLHLIREELEISQK
                     ELAATLGIKQPSLSAIENRGHDLKISTMKKYVEAMGGKLRIDVELPTGKHIGFNV"
                     /name="helix-turn-helix domain-containing protein"
     gene            411..620
                     /locus_tag="LO002_RS19440"
     CDS             411..620
                     /locus_tag="LO002_RS19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000069473.1"
                     /translation="MSNNKPLKNSTKLLVNLDKFIFLVNAADSLEEIEIIRDLCCDYFS
                     NCKRSSYYIDIFDDAYWIKYYEIA"
                     /name="hypothetical protein"
     gene            complement(942..1402)
                     /locus_tag="LO002_RS19445"
                     /pseudo=""
     CDS             complement(942..1402)
                     /locus_tag="LO002_RS19445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /name="DIP1984 family protein"
     gene            complement(1707..4118)
                     /gene="znuD2"
                     /locus_tag="LO002_RS19450"
     CDS             complement(1707..4118)
                     /gene="znuD2"
                     /locus_tag="LO002_RS19450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            complement(4246..4560)
                     /locus_tag="LO002_RS19455"
     CDS             complement(4246..4560)
                     /locus_tag="LO002_RS19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            complement(4547..4835)
                     /locus_tag="LO002_RS19460"
                     /pseudo=""
     CDS             complement(4547..4835)
                     /locus_tag="LO002_RS19460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /name="BrnT family toxin"
     gene            5144..5971
                     /locus_tag="LO002_RS19465"
     CDS             5144..5971
                     /locus_tag="LO002_RS19465"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            complement(6015..6206)
                     /locus_tag="LO002_RS19470"
     CDS             complement(6015..6206)
                     /locus_tag="LO002_RS19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000032259.1"
                     /translation="MSKELVNLANIVPFPKSCGFCESKENVQLFAGLMLCSSCQENIRT
                     TNPGMFTANKQIEKKAQD"
                     /name="hypothetical protein"
     gene            complement(6440..6658)
                     /locus_tag="LO002_RS19550"
     CDS             complement(6440..6658)
                     /locus_tag="LO002_RS19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002031357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_015062952.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLKMMTFKALFHKVFGCFYRF"
                     /name="hypothetical protein"
     gene            7342..8292
                     /gene="repM"
                     /locus_tag="LO002_RS19475"
     CDS             7342..8292
                     /gene="repM"
                     /locus_tag="LO002_RS19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            8285..8860
                     /locus_tag="LO002_RS19480"
     CDS             8285..8860
                     /locus_tag="LO002_RS19480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            8880..9023
                     /locus_tag="LO002_RS19485"
     CDS             8880..9023
                     /locus_tag="LO002_RS19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(9206..10033)
                     /locus_tag="LO002_RS19490"
     CDS             complement(9206..10033)
                     /locus_tag="LO002_RS19490"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_057689930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /protein_id="WP_000713530.1"
                     /translation="MKKFILPIFSISILVSLSACSSIKTKSEDNFHISSQQHEKAIKSY
                     FDEAQTQGVIIIKEGKNLSTYGNALARANKEYVPASTFKMLNALIGLENHKATTNEIFK
                     WDGKKRTYPMWEKDMTLGEAMALSAVPVYQELARRTGLELMQKEVKRVNFGNTNIGTQV
                     DNFWLVGPLKITPVQEVNFADDLAHNRLPFKLETQEEVKKMLLIKEVNGSKIYAKSGWG
                     MDVTPQVGWLTGWVEQANGKKIPFSLNLEMKEGMSGSIRNEITYKSLENLGII"
                     /name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
     gene            10342..10629
                     /locus_tag="LO002_RS19495"
     CDS             10342..10629
                     /locus_tag="LO002_RS19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            10616..10930
                     /locus_tag="LO002_RS19500"
     CDS             10616..10930
                     /locus_tag="LO002_RS19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            11058..13468
                     /gene="znuD2"
                     /locus_tag="LO002_RS19505"
                     /pseudo=""
     CDS             11058..13468
                     /gene="znuD2"
                     /locus_tag="LO002_RS19505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /name="znuD2"
     gene            13773..14234
                     /locus_tag="LO002_RS19510"
     CDS             13773..14234
                     /locus_tag="LO002_RS19510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(14559..>14741)
                     /locus_tag="LO002_RS19515"
                     /pseudo=""
     CDS             complement(14559..>14741)
                     /locus_tag="LO002_RS19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680077.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     ARG             5145..5968
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(9210..10033)
                     /name="blaOXA-72"
                     /gene="blaOXA-72"
                     /gene_name="OXA-24 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-72"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     Typing          7343..8292
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctattgtg ctgtgtatcg gtgataaagg cggtaaaagc gtttttataa agagatgtta
       61 gatattgctg atcagcaata cgaacttcat ctctcaactt taggagatca atctaatggc
      121 taagacccta caagaattat tggctagtcg ctctccagag agtcaagctc gtattcaaca
      181 aatggctgat gaattactgt tggaaactca gcttcacctt attcgtgaag agttagagat
      241 ttctcaaaaa gaactggctg caacccttgg aatcaaacag ccgtcattgt cagcaatcga
      301 aaatcgtggt catgatctta aaatttcaac catgaaaaag tatgttgaag caatgggcgg
      361 aaaacttcgt attgatgtag agcttccaac aggaaaacac ataggattta atgtctaata
      421 ataaaccttt gaaaaatagt actaaattac ttgttaactt ggacaagttc atatttttag
      481 tcaatgctgc cgatagccta gaagaaattg aaattattag agatttatgc tgtgactatt
      541 tttcaaattg taagaggtca agctattaca ttgatatttt tgacgatgct tattggataa
      601 aatattacga aatagcttaa tccttccaga ttcgcataag agattttgtt aaatgccgaa
      661 ctgaattaaa aatagaaatg tagttgagct gatgcttgtt catttcttta tttttagtaa
      721 ttgaggtgaa catactgtca gtacatggta gttataagta acgtgtaact ttgtttgcac
      781 aaagatttga gttatgccaa gtgatggatt gatttactta aaattttaag cttatccact
      841 tgctttgcaa gccactacac tttatttaca atcctatgtt gtggaagtaa gggatcttgt
      901 actcgcccag tatgttcaac ttgattatat ctaaaggact attaggcttc aatcaaatca
      961 attttccaat tagtcgactg aattaacacg tttaaatcac gtaatttaag actaatatca
     1021 tcagcttgtt tttgtaatga tgaaactgga ataactttat gccactttat ttcccgcgta
     1081 ctataacgat cagattcacg agatgtatta cttattgctg cagttagggt tttgtgctgt
     1141 tctacaaatt catcacgcaa agtcaataaa gcaagtagag aacggccatc atttaatttt
     1201 ttaaagcatt agtttcatga attgcataac ttaatttttg aaattcttga attaaagaaa
     1261 atacttgttt tagtagttca tttggatctt cagatggttg atcaccatct tgtaccaata
     1321 catttgcatt gatacgttgt tttagtgaaa taattttttt ttgttgatca cttcgaagta
     1381 agagagcttc tgctaatttc atttttcacc tattaaccaa ttagaattaa gttttaaaca
     1441 gcttatatcc tcattcttta aagatcaaaa aaattgcttt ttatctagct aaaaggtgtc
     1501 tacaaaatcg gtggctattc agtgctgaaa aggctgccga cacataaagt gacccagatt
     1561 gaagagttac tgccacactg ctggaaacct aaatcgaatt aaaaaatagg tatgggattc
     1621 agcggacgct taccttaatt attaatctaa tcattttata agaaaaatct tctaagaaat
     1681 tttcttagaa gattacagta tcttatctag aaattaagat ttaagcctaa actaaaattt
     1741 cttccaattt ggggaatata tggcaaaaat gtttcatggg catagacttt ctgatcaagt
     1801 aaattattcg ctttaaagaa taaatcatat tctgtgtgag atagcttatt tttatatgct
     1861 aaagtcatat taagcatatt ataacctgat gtaacctgct caaatttaga aatattatct
     1921 tgtttaaaga ctcgataata ttctatttca cctttaagcg attcatcaaa atcagctttt
     1981 agtcgtgtac ccaaacgagc aggaggtaat ctaggagtgt aacgatcctt ttggggagct
     2041 aaagtcggtt ctctattcca gatatcgtat gcaattacag catcaggtaa atttgttaaa
     2101 cggcctttca cataatctcc aaaaagagag ccgtgataaa cagaattaaa ttgataccct
     2161 atgttgccct ctaatccata aaaattagca gcagactggc tataatgatt aatacgtagt
     2221 gtatgctgat ctctaacttt tgtagttcct aaaacttcat tgagtgtctg taaatatata
     2281 tagttatcaa aatcataata gtaagtactg atttgataat caagtaaatc atccttatag
     2341 gccagactga tttctaaatt attggatttc tcttttctta gaaagcggtt acctatttca
     2401 aaagaattag tagcgagatg cataccatga gtatacattt cttgtgcatt aggtaatctt
     2461 tcttgatgtg tacctgttaa tgataagata taatttggag caaatctcca gtggactgta
     2521 cctgcataag aaaaagctgt ttctttgtta ggttgaacag cttctaaaat agattttaaa
     2581 ttttgagccc tgattttatt atttttttct acataaggac tgttatattt attaggccaa
     2641 ggtttcattg aatctttaat tttttctata tcataatcca tactaacttt ctgcttttct
     2701 atacgagcac ctagttcgaa agtaaagtcg ttccagttat atttttctag gccaaataca
     2761 ctaaagtttt tagtgacatt tcgatttaat agctgctgat tatctagata ggtggtatgg
     2821 ccttcttgag aatggactgg tgataaagca ctattatcct gttccaaata ttgtagcccc
     2881 aagataccgg ttaattcccc taatggctga tgtgataact ctatgcggcc tacttttcct
     2941 gtatttttaa aaaagttagt aatcttatct ccctcgagtt cattgtgaaa ataatctata
     3001 taactcaatg aggtacgaat tttgtcaata ccggtaaatg gttgagtgaa ttctccacgc
     3061 acatcataac gtcgagtatt taaatcaata taaggagcat tatgagagaa attagtttca
     3121 tgaatatgat tatgctgatg acaatcagga cgtggattta tgtaattaat atcttgctca
     3181 tccatcaatt gaggataata taaaagataa ttttttaatc cactaattcg tgaatcgata
     3241 ccgattgcag aggcaccaca accttcatat aaatggctat gggctggtaa accatattca
     3301 tcatgacgat gtgtataaga aactcctaaa taagtattat cgtctatcca ggataatcca
     3361 agcgtaccta ctctcgactc tgcccagctt tctggtaaat attttaaatt tttataaata
     3421 ctgttttggt tttcaagatc ttctaaagaa ttataagagc tagactggta atgaggagtt
     3481 ttatagttac cagcattacg atataaccct tctgcccgta aagctacatg tgggcttaag
     3541 ccaactgtta cacctgctgt ggtcaacttc tcattactgc cagtattaaa acgtaatgta
     3601 gtattaccct ctaatccgtc ttgaggcatt tgggttggaa ttttatagtc aatgacattg
     3661 actacccctg ccgcactatt tgaactatac aaaagggtac ttgcaccacg tataatttca
     3721 atagacttag ctaaacttgg ttcaactgtg acagcgtgat ctggggacat attagacata
     3781 tccaacacgt cagcattatt ctgtaaaact ttaatacgtt ttccttcctg cccacgaata
     3841 ataggagtag atgctccacc tccatattga ttagaatgaa ttcctaattc cgtacccaag
     3901 gcatccccta gagttgtcga acgctgttta agtttactag tatcaataaa ctgattacta
     3961 acatttagac ttttgcctaa cagctcagac cctaactcat cagattgtgc agttaatact
     4021 attgtattta aggtagtaac aggcccttca gctgccaata aaattttcga ggcccctaaa
     4081 gtaattaaag ctaaaaatag tttactttta tttagcataa aacaaccaaa tttaatgtta
     4141 tattataata taacacttaa aaatttataa aatctactat ttaaattttt gaatggtaat
     4201 cccccctaaa aatgaagtca aaaatgggag cctaagctcc cattcttatt gatgattttt
     4261 taattctttt agcatagcat ctcgtaaaat ttcattcata cgtgtttgat aacctttgcc
     4321 ttgtgctttg aaccaagcaa gtacatcagc atctaaccga attgaagttt gttgctttac
     4381 tgggcggtaa aattgatttt ggcgtacagc attgctccag tctgtaattt caggaatatc
     4441 tgatagatct agctgatcat caggaaccgt gcctttagca agcaagcgtt gaatttcagc
     4501 atcttgcttc tcaccaaatt tctcattcag ctctttgtgt gagtatctaa ccatgctcat
     4561 atttgtttcg ctccgcttta gtgacttgcc ttgcactaat gattcggatg atttcacagt
     4621 catcttcatc aaaaatggtg tgagccacca ataacattag tacgccttta actcgtccaa
     4681 tggtttgcca aacgttcttc accattggta tgtctgtctt ggattgagat ccgtaatgga
     4741 tcttcaaaaa caaggcttgc agtttcgaaa gagatatcat gctttttctg attctttcga
     4801 ttcttagcct catcccattc aaaatactgt tccataaaaa acattcaaat ttgtatatac
     4861 aataatgtat cacaaaattg tatcactcaa ccagattcgt gtaattttta gtcaaaatta
     4921 ttggcaagaa gtatcaaaac tgctcataaa aaagccgact tgtttcaaag tcggcttttt
     4981 gggcattttg ggatttttct atttttatta agtagttgat atttatatta atatacaagt
     5041 gtacttcgta taatcgccat tatgttaaat taaaagagtt ttaacttttt taataaaaat
     5101 attatactct aagccccaaa atttccccta acatgaattt gtaatgaaaa aatttatact
     5161 tcctatattc agcatttcta ttctagtttc tctcagtgca tgttcatcta ttaaaactaa
     5221 atctgaagat aattttcata tttcttctca gcaacatgaa aaagctatta aaagctattt
     5281 tgatgaagct caaacacagg gtgtaattat tattaaagag ggtaaaaatc ttagcaccta
     5341 tggtaatgct cttgcacgag caaataaaga atatgtccct gcatcaacat ttaagatgct
     5401 aaatgcttta atcgggctag aaaatcataa agcaacaaca aatgagattt tcaaatggga
     5461 tggtaaaaaa agaacttatc ctatgtggga gaaagatatg actttaggtg aggcaatggc
     5521 attgtcagca gttccagtat atcaagagct tgcaagacgg actggcctag agctaatgca
     5581 gaaagaagta aagcgggtta attttggaaa tacaaatatt ggaacacagg tcgataattt
     5641 ttggttagtt ggccccctta aaattacacc agtacaagaa gttaattttg ccgatgacct
     5701 tgcacataac cgattacctt ttaaattaga aactcaagaa gaagttaaaa aaatgcttct
     5761 aattaaagaa gtaaatggta gtaagattta tgcaaaaagt ggatggggaa tggatgttac
     5821 tccacaggta ggttggttga ctggttgggt ggagcaagct aatggaaaaa aaatcccctt
     5881 ttcgctcaac ttagaaatga aagaaggaat gtctggttct attcgtaatg aaattactta
     5941 taagtcgcta gaaaatcttg gaatcattta ataatttcta aaattaacat aatacacctt
     6001 atgcgaagcc cagattaatc ctgggctttt ttttcaattt gcttattagc tgtaaacatt
     6061 ccaggattag tggttcggat gttttcctga cagctactac aaagcattaa ccctgcaaaa
     6121 agttgtacat tttcctttga ttcacagaat ccacatgatt taggaaaagg aacgatattt
     6181 gctaagttga ctaattcttt tgacataatg tttatcctca acttgtacat attaatttca
     6241 atatttttca gtatgttata cctaatttca cgttaaactt tgtacattct tgtataatcc
     6301 atgtgtacaa actctttttt ataaatttca tatatttaag cctaaaaaac cattaattta
     6361 tgtacaacac tatgtacata ctaaaaacta tataaatcaa aaaattagta ttaaaaagat
     6421 caattatttt gtacacaatt cagaacctat aaaaacaacc aaaaacctta tgaaataaag
     6481 ctttgaaggt catcatcttt agatgcgtgg tgtgtgacaa aaaaatcccg gcatgtgccg
     6541 gatcttggat tggaaaattg gctaaagtga cgtagggccg gtgcttggtt ttgcatggaa
     6601 aaaagtattt atttccttgt ttagaaaaac gtaaaaaatc aattttttgt ccattcatcc
     6661 aggttaaaaa tttcaaccca aaactttaat tatgaaaagc ttcatagaac gcgttcaaat
     6721 gcgatttaag agcctttacc tagaaaacat agatctcata gtgaaaaaca caaaacagct
     6781 caaaaaacgc aaatgagagc gaaataaaga ggtattttga ctttagatag atgcatagag
     6841 cgagtgtcta cgagcgaact atcaaaattt gcgcctagac cctctgaaaa acattttttt
     6901 gtcctcttta gcctaagaaa gcgtaagttt cgtgcagaaa tttgctcctg gatcgagcgt
     6961 agcgagtaaa aaagttttat gagcgaagcg aattccaagt ttcttttgct tttttttaaa
     7021 gtcacgccag tattaaccaa aaaattgccc caacgaactg agcgaaagcg aagttcaata
     7081 gagtttgagc gaagcgaaaa ccaagggcaa tttttcattc cctgggcttt taattatttt
     7141 aaagttttta aatactttta aatggcttga aagccttatg aataaagggt tttagctctt
     7201 atatgtccac gtttaccttg caatatgtcc acgtttacct tgcaatatgt ccacgtttac
     7261 cttgcaatat gtccacgttt accttgcatt agtacacaaa taatattaac gtgtacttat
     7321 acacaataaa aaatagtggc tatgagagat ttagttgtaa aggacaatgc cttaatcaac
     7381 gcaagctata acttagactt agtagaacaa cgtttaattt tattggctat tgttgaagca
     7441 agggaaagtg ggaaagggat taatgcaaat gatccattag aagttcatgc agatagttat
     7501 atcaatcaat ttggtgtaca ccgtaataca gcttatcaag ccttaaaaga tgcttgtaag
     7561 gatttattcg cgcgtcaatt tagctatcaa gagaaaaaag ctaatgggaa tatccgaaat
     7621 gttatgagtc gttgggtatc tcaaattgct tataacgaca atgaagcaac tgtagactta
     7681 atatttgcac ctgctgttgt tcccttcata acccgactgg aagaacaatt tactaaatat
     7741 gaattacagc aagttagtag tcttagtagc gcttatgcca ttcgcttata tgagcttcta
     7801 attcagtggc gaagcactgg taaaacccca accatagaac tacaagaatt tagaaagaag
     7861 ttaggcgttc ttgataatga atatttacgg atggctcatt taaaagagcg tgttttagag
     7921 ctttcaatta aacaaataaa tgagcatacg gatataactg taaaatatga acagcataaa
     7981 agaggacgtt ctatttcagg attttctttt acctttaaac agaagaagaa ggatagccca
     8041 tcaatagaaa gagatccgaa cactttggag cttttttcaa agatgaccga tgctcaacgg
     8101 catatgtttg caaataaact ttcagaactc cctgaaatgg gtcgctattc acaaggaaca
     8161 gaaagctacc aacagtttgc tgtacgtatt gctgagatgc tacaagatcc cgctcaattc
     8221 aaagagctat acccatacct aaaaaaagtg ggatacatgc catcaaataa aaaggacacc
     8281 gtaaatggct aagttatcac taagtgaagt atctaaaaaa tttcatgtgg atagatcaac
     8341 catttacaga gctgtacgta atggacgttt atcacgctcc agtgatggac aattcgatct
     8401 agcagaggtc atacgatgct ttggagaacc tgagcaaaca tctcaaaaaa ttgaatcatc
     8461 taagcaagaa ggtgatgaat ctacaaaaaa acttattgcc catttagaaa atgaagtcaa
     8521 aaaataccaa gaacgtgaag aacggttaat gcaacaaatt gaccgtatgc aaacactcat
     8581 tgagctgaaa agtgttgcac ctgccacagc agcaccacac caagatgcta cggcatgcga
     8641 caccaagatg ccacagcatg cgacaacaca acaagacact gataacaaaa agaataatga
     8701 attaaatatt gcagaaaatg tagcagtgcc acagcaagaa actacggcat accacaccca
     8761 aacgctacag catgccacgt tgcaaagtgt ggcagtgcca caacacaaaa aacgtggttt
     8821 atttggccgt gtgctgaatg ccgtctttga taatgactga gcaataaggg tgaaagatca
     8881 tgccgaaact gaaagacatt gccctgggaa ttattgtagc tccgctgctg atcccgatca
     8941 tgctgattgc atcgtaccag gataaaaagg cgctgaagaa agagctggat gaatgccaaa
     9001 aagaaaaaga ccaggcatcc tgattattca attgagctaa tcaaaagtgt ggaacattga
     9061 tgaaataata gcgtggaacg tgaaaagtca taaaaaaagc ccgaacaggg atgctcgggc
     9121 tataaactag aaactacagc gttgaattat aaggataatt atacagcatt tcgtataagg
     9181 tgtattatgt taattttaga aattattaaa tgattccaag attttctagc gacttataag
     9241 taatttcatt acgaatagaa ccagacattc cttctttcat ttctaagttg agcgaaaagg
     9301 ggattttttt tccattagct tgctccaccc aaccagtcaa ccaacctacc tgtggagtaa
     9361 catccattcc ccatccactt tttgcataaa tcttactacc atttacttct ttaattagaa
     9421 gcattttttt aacttcttct tgagtttcta atttaaaagg taatcggtta tgtgcaaggt
     9481 catcggcaaa attaacttct tgtactggtg taattttaag ggggccaact aaccaaaaat
     9541 tatcgacctg tgttccaata tttgtatttc caaaattaac ccgctttact tctttctgca
     9601 ttagctctag gccagtccgt cttgcaagct cttgatatac tggaactgct gacaatgcca
     9661 ttgcctcacc taaagtcata tctttctccc acataggata agttcttttt ttaccatccc
     9721 atttgaaaat ctcatttgtt gttgctttat gattttctag cccgattaaa gcatttagca
     9781 tcttaaatgt tgatgcaggg acatattctt tatttgctcg tgcaagagca ttaccatagg
     9841 tgctaagatt tttaccctct ttaataataa ttacaccctg tgtttgagct tcatcaaaat
     9901 agcttttaat agctttttca tgttgctgag aagaaatatg aaaattatct tcagatttag
     9961 ttttaataga tgaacatgca ctgagagaaa ctagaataga aatgctgaat ataggaagta
    10021 taaatttttt cattacaaat tcatgttagg ggaaattttg gggcttagag tataatattt
    10081 ttattaaaaa agttaaaact cttttaattt aacataatgg cgattatacg aagtacactt
    10141 gtatattaat ataaatatca actacttaat aaaaatagaa aaatcccaaa atgcccaaaa
    10201 agccgacttt gaaacaagtc ggctttttta tgagcagttt tgatacttct tgccaataat
    10261 tttgactaaa aattacacga atctggttga gtgatacaat tttgtgatac attattgtat
    10321 atacaaattt gaatgttttt tatggaacag tattttgaat gggatgaggc taagaatcga
    10381 aagaatcaga aaaagcatga tatctctttc gaaactgcaa gccttgtttt tgaagatcca
    10441 ttacggatct caatccaaga cagacatacc aatggtgaag aacgttggca aaccattgga
    10501 cgagttaaag gcgtactaat gttattggtg gctcacacca tttttgatga agatgactgt
    10561 gaaatcatcc gaatcattag tgcaaggcaa gtcactaaag cggagcgaaa caaatatgag
    10621 catggttaga tactcacaca aagagctgaa tgagaaattt ggtgagaagc aagatgctga
    10681 aattcaacgc ttgcttgcta aaggcacggt tcctgatgat cagctagatc tatcagatat
    10741 tcctgaaatt acagactgga gcaatgctgt acgccaaaat caattttacc gcccagtaaa
    10801 gcaacaaact tcaattcggt tagatgctga tgtacttgct tggttcaaag cacaaggcaa
    10861 aggttatcaa acacgtatga atgaaatttt acgagatgct atgctaaaag aattaaaaaa
    10921 tcatcaataa gaatgggagc ttaggctccc atttttgact tcatttttag gggggattac
    10981 cattcaaaaa tttaaatagt agattttata aatttttaag tgttatatta taatataaca
    11041 ttaaatttgg ttgttttatg ctaaataaaa gtaaactatt tttagcttta attactttag
    11101 gggcctcgaa aattttattg gcagctgaag ggcctgttac taccttaaat acaatagtat
    11161 taactgcaca atctgatgag ttagggtctg agctgttagg caaaagtcta aatgttagta
    11221 atcagtttat tgatactagt aaacttaaac agcgttcgac aactctaggg gatgccttgg
    11281 gtacggaatt aggaattcat tctaatcaat atggaggtgg agcatctact cctattattc
    11341 gtgggcagga aggaaaacgt attaaagttt tacagaataa tgctgacgtg ttggatatgt
    11401 ctaatatgtc cccagatcac gctgtcacag ttgaaccaag tttagctaag tctattgaaa
    11461 ttatacgtgg tgcaagtacc cttttgtata gttcaaatag tgcggcaggg gtagtcaatg
    11521 tcattgacta taaaattcca acccaaatgc ctcaagacgg attagagggt aatactacat
    11581 tacgttttaa tactggcagt aatgagaagt tgaccacagc aggtgtaaca gttggcttaa
    11641 gcccacatgt agctttacgg gcagaagggt tatatcgtaa tgctggtaac tataaaactc
    11701 ctcattacca gtctagctct tataattctt tagaagatct tgaaaaccaa aacagtattt
    11761 ataaaaattt aaaatattta ccagaaagct gggcagagtc gagagtaggt acgcttggat
    11821 tatcctggat agacgataat acttatttag gagtttctta tacacatcgt catgatgaat
    11881 atggtttacc agcccatagc catttatatg aaggttgtgg tgcctctgca atcggtatcg
    11941 attcacgaat tagtggatta aaaaattatc ttttatatta tcctcaattg atggatgagc
    12001 aagatattaa ttacataaat ccacgtcctg attgtcatca gcataatcat attcatgaaa
    12061 ctaatttctc tcataatgct ccttatattg atttaaatac tcgacgttat gatgtgcgtg
    12121 gagaattcac tcaaccattt accggtattg acaaaattcg tacctcattg agttatatag
    12181 attattttca caatgaactc gagggagata agattactaa cttttttaaa aatacaggaa
    12241 aagtaggccg catagagtta tcacatcagc cattagggga attaaccggt atcttggggc
    12301 tacaatattt ggaacaggat aatagtgctt tatcaccagt ccattctcaa gaaggccata
    12361 ccacctatct agataatcag cagctattaa atcgaaatgt cactaaaaac tttagtgtat
    12421 ttggcctaga aaaatataac tggaacgact ttactttcga actaggtgct cgtatagaaa
    12481 agcagaaagt tagtatggat tatgatatag aaaaaattaa agattcaatg aaaccttggc
    12541 ctaataaata taacagtcct tatgtagaaa aaaataataa aatcagggct caaaatttaa
    12601 aatctatttt agaagctgtt caacctaaca aagaaacagc tttttttatg caggtacagt
    12661 ccactggaga tttgctccaa attatatctt atcattaaca ggtacacatc aagaaagatt
    12721 acctaatgca caagaaatgt atactcatgg tatgcatctc gctactaatt cttttgaaat
    12781 aggtaaccgc tttctaagaa aagagaaatc caataattta gaaatcagtc tggcctataa
    12841 ggatgattta cttgattatc aaatcagtac ttactattat gattttgata actatatata
    12901 tttacagaca ctcaatgaag ttttaggaac tacaaaagtt agagatcagc atacactacg
    12961 tattaatcat tatagccagt ctgctgctaa tttttatgga ttagagggca acatagggta
    13021 tcaatttaat tctgtttatc acggctctct ttttggagat tatgtgaaag gccgtttaac
    13081 aaatttacct gatgctgtaa ttgcatacga tatctggaat agagaaccga ctttagctcc
    13141 ccaaaaggat cgttacactc ctagattacc tcctgctcgt ttgggtacac gactaaaagc
    13201 tgattttgat gaatcgctta aaggtgaaat agaatattat cgagtcttta aacaagataa
    13261 tatttctaaa tttgagcagg ttacatcagg ttataatatg cttaatatga ctttagcata
    13321 taaaaataag ctatctcaca cagaatatga tttattcttt aaagcgaata atttacttga
    13381 tcagaaagtc tatgcccatg aaacattttt gccatatatt ccccaaattg gaagaaattt
    13441 tagtttaggc ttaaatctta atttctagat aagatactgt aatcttctaa gaaaatttct
    13501 tagaagattt ttcttataaa atgattagat taataattaa ggtaagcgtc cgctgaatcc
    13561 catacctatt ttttaattcg atttaggttt ccagcagtgt ggcagtaact cttcaatctg
    13621 ggtcacttta tgtgtcggca gccttttcag cactgaatag ccaccgattt tgtagacacc
    13681 ttttagctag ataaaaagca atttttttga tctttaaaga atgaggatat aagctgttta
    13741 aaacttaatt ctaattggtt aataggtgaa aaatgaaatt agcagaagct ctcttacttc
    13801 gaagtgatca acaaaaaaaa attatttcac taaaacaacg tatcaatgca aatgtattgg
    13861 tacaagatgg tgatcaacca tctgaagatc caaatgaact actaaaacaa gtattttctt
    13921 taattcaaga atttcaaaaa ttaagttatg caattcatga aactaatgct ttaacaaaat
    13981 taaatgatgg ccgttctcta cttgctttat tgactttgcg tgatgaattt gtagaacagc
    14041 acaaaaccct aactgcagca ataagtaata catctcgtga atctgatcgt tatagtacgc
    14101 gggaaataaa gtggcataaa gttattccag tttcatcatt acaaaaacaa gctgatgata
    14161 ttagtcttaa attacgtgat ttaaacgtgt taattcagtc gactaattgg aaaattgatt
    14221 tgattgaagc ctaatagtcc tttagatata atcaagttga acatactggc gagtacaaga
    14281 tcccttactt ccacaacata ggattgtaaa taaagtgtag tggcttgcaa agcaagtgga
    14341 taagcttaaa attttaagta caaatcaatc catcacttgg cataactcaa atctttgtgc
    14401 aaacaaagtt acacgttact tataactacc atgtactgac agtatgttca cctcaattac
    14461 taaaaataaa gaaatgaaca agcatcagct caactacatt tctattttta attcagttcg
    14521 gcatttaaca taaaatctct tatgcgaatc tggaaggatt aagctatttc gtaatatttt
    14581 atccaataag catcgtcaaa aatatcaatg taatagcttg acctctacaa ttgaaaaata
    14641 gtcacagcat aaatctctaa taatttcaat ttcttctagg ctatcggcag cattgactaa
    14701 aaatatgaac ttgtccaagt taacaagtaa tttagtacta t
//
