LOCUS       NZ_CP087315            14545 bp    DNA     circular CON 12-SEP-2023
DEFINITION  Acinetobacter baumannii strain OC059 plasmid p3OC059, complete
            sequence.
ACCESSION   NZ_CP087315
VERSION     NZ_CP087315.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN23078459
            Assembly: GCF_024749525.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 14545)
  AUTHORS   Mellmann,A.
  TITLE     Sequencing of Acinetobacter baumannii genomes to construct genome
            database for SNP detection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14545)
  AUTHORS   Mellmann,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2021) Inst. Hygiene, University Hospital Muenster,
            Robert-Koch-Str. 41, Muenster 48149, Germany
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: HGAP v. 4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 422x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 09/12/2023 05:46:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,982
            CDSs (total)                      :: 3,887
            Genes (coding)                    :: 3,810
            CDSs (with protein)               :: 3,810
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 24 of 77
            Pseudo Genes (incomplete)         :: 48 of 77
            Pseudo Genes (internal stop)      :: 17 of 77
            Pseudo Genes (multiple problems)  :: 11 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087315.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..14545
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="OC059"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="p3OC059"
                     /geo_loc_name="Germany"
     gene            216..1766
                     /locus_tag="LO007_RS19830"
     CDS             216..1766
                     /locus_tag="LO007_RS19830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101605.1"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]; GO:1904047 - S-adenosyl-L-methionine
                     binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="WP_188137490.1"
                     /translation="MARAPKKETKQEPLEVVLWKTADKLRKNIDAAEYKHVVLGLIFLK
                     YISDSFEAHYNLLVLGEGEFAGADPEDRDEYTAYNVFFVPENARWSFLLNQAKQPNIGK
                     LVDDAMEAIENDNPQLKGVLPKVYARQNLDPTSLGELIDLIGNIALGDAKSRSADVLGH
                     VFEYFLGEFALAEGKQGGQFYTPKSIVSLLVNMLEPYKGRVFDPCCGSGGMFVQSEKFV
                     ESHQGLVDDISIYGQESNQTTWRLAKMNLAIRGINSEHVKWNSEGSFLNDAHKDLKADY
                     IIANPPFNVSDWSGEQLVGDARWQLGTPPAGNANFAWMQHFLYHMSPKGQAGVVLAKGA
                     LTSKTSGEGEIRKALVSEANVIDCIVNLPAKLFLNTQIPAALWFMRRDRVNSTKYKDRS
                     NEILFIDARNLGHLINRRTKVLSDEDIKLITDTYHNWRNKDGEYEDVAGFCASVPLEKV
                     AELDYVLTPGRYVGLADDEDEFDFKERFTSLKAEFEAQLAEETKLNQAIAENLAKVIL"
                     /name="class I SAM-dependent DNA methyltransferase"
     gene            1763..2992
                     /locus_tag="LO007_RS19835"
     CDS             1763..2992
                     /locus_tag="LO007_RS19835"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006304 - DNA modification [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="WP_188137489.1"
                     /translation="MSEWSITKLGDVLEITNGYAFKSVNFRSEPEAGCLPVLKIKNVAN
                     GDVHINDVQYHHFEEKLSKYTAQKGDILISLTGNHPHAETQVVGAVSKLKMDIRAFVNQ
                     RVAKIDTKPNLADKDYIYFALKEEGLSKYLASQSSGSANQANISKADIEDLDIALPPLP
                     EQKTIASVLSSLDDKIDLLHRQNKTLEAMAETLFRQWFIEEAKEDWEIFQLQDLAKLQI
                     GRTPPRKESHWFSDNTNDMKWVSIKDLGAHGIFVTYSSEYLPISTIDHFNIPIIPEGTV
                     MLSFKMTVGRVGIAIENMVSNEAIAQFQIKKRLTSELLYCFLKQFNFDTLGSTSSIVTA
                     VNTALLKGIEIALPEDDLIQEFTEQVKSYFDKVKANQLQIQTLEKLRDTLLPKLMSGEV
                     RVQYQTEEVA"
                     /name="restriction endonuclease subunit S"
     gene            3046..4074
                     /locus_tag="LO007_RS19840"
     CDS             3046..4074
                     /locus_tag="LO007_RS19840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005215812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2939 domain-containing protein"
                     /protein_id="WP_188137488.1"
                     /translation="MNKKLIGALITVIVVLVGGYYYASPYIVLNSIKSAAQAGDSEKVS
                     AYIDYSSVRQSFKDQMNAYMVKDLASKDTDGWEALGTMIATTMIDKMVDAVVTPEGMTL
                     MLQGKDFKESLKPHTEQSSDQTTSKEKLDYSTRYLSMNLFEVTLKNPNNDKQLKVIMER
                     DGLSWKVKKFVIPMDDMETNQTTEQQPTLPVEEILAETEAPTIIEQPETVFDYSGVQKG
                     AQLESCYHDPCSIAKVMGFKLINQTPTESDLELTVVGGSRGFESESIEWNHEAHKIQIH
                     CSITKPTLQIGDQVTVIPLNDSGVPGVLWSDAETYMQACHNYTGEMRAGIQQYGYNVQS
                     YE"
                     /name="DUF2939 domain-containing protein"
     gene            4149..7394
                     /locus_tag="LO007_RS19845"
     CDS             4149..7394
                     /locus_tag="LO007_RS19845"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004762885.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016887 - ATP hydrolysis activity [Evidence IEA];
                     GO:0003677 - DNA binding [Evidence IEA]; GO:0004386 -
                     helicase activity [Evidence IEA]; GO:0009035 - type I
                     site-specific deoxyribonuclease activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="WP_188137487.1"
                     /translation="MTKMTESDIELMAIEKLEGMGYTYVYGPDIESKGSNPLRSYKQVI
                     LEAKVLEALQRLNLHLNEDKCIEALKQITNLVSPDLLTNNQAFHRLLTEGVNIEVSKDG
                     NTQGEYAWLIDFNNPSNNEFLVVNQVTIREDRWERRPDIILYVNGLPLVVIELKNATDE
                     NATVEGAYKQIQTYQSQIPSLFTYNAFNIISDGLEAKAGTVSADLSRYMAWKTTNGQTK
                     AKKTQAQLEVLIHGLLNPVTLLDMIRHFIVFESNKQEDANGLITIKTIKKMAAYHQYYA
                     VNAAVLSTIRASAVNSDSKSAEVAMQHQGRSKLELVQQQAVGDKKAGVVWHTQGSGKSL
                     SMVFYTGKIVLALDNPTVVVITDRNDLDDQLFGTFAASSQLLRQTPKQADSREELKELL
                     KVGSGGVIFTTIQKFQPDDGGSVYEQLSDRTNIVVIADEAHRSQYGFNAKEVDVKDENG
                     KVVGKRTVYGFAKYMRDALPNATYLGFTGTPIEKTDVNTPAVFGNYVDIYDISQAVEDG
                     ATVRIFYESRLAKIAISDEGKELIEDFDDQFTEDDLNQTQKERAKWSRVEGLIGSKERI
                     KAIAKDIVEHFEQRLKANANQGKGMIVSMSRRIAVDLYNEIVAIKPEWHSDDLHDGVIK
                     VVMTSSAADGEEIAKHHTTKQQRQILANRMKDNDDKLKLVIVRDMWLTGFDAPSMHTLY
                     IDKPMKGHNLMQAIARVNRVYKDKIGGLVVDYLGIASDLKEALSFYSEAGGKGDPALVQ
                     DEAATILQEKLEVIDGIMHGFNYQEYFTANTSRKLNIILEAEEHILGVDQGEGKTRFLT
                     AVAAMSQAFALAVPHPHAMEAAPVVAFFQAVKARLAKFSDGSGNVSGMSNAELEAKVKQ
                     TIDQALVTEQVVDIFDAAGIQKPDISILSDEFMQEMKGYKHKNIALETLKKLLSDEIKV
                     REQRSVTQGKKLMDMLSSAIKGYQNKVLTAAEVIDELIKLAKAIQESDKMAEELNLTEY
                     EYAFYSAVADNDSARELMQKDILRELAVVLTESIRKNVTLDWTIKEAARAKLRVIVKRL
                     LKKYGYPPDMALLATETVLEQAEQLADELSKAA"
                     /name="type I restriction endonuclease subunit R"
     gene            complement(7465..8379)
                     /gene="repM"
                     /locus_tag="LO007_RS19850"
     CDS             complement(7465..8379)
                     /gene="repM"
                     /locus_tag="LO007_RS19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016658926.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_188137486.1"
                     /translation="MKNDLVVKDNALINASYNLDTTEQRLILLAIVQARELSKNVDANS
                     TLEVHAHHYMKQFNVDKHAAYEGLKNAASNLFERKFSYKGIHEGTQQEKIVKSRWVSKI
                     AYVDSAGIVELTFAPDVIPLITQLEKSFTAYELKQISSLTSKYAIRLYELLIQWRSVGK
                     TPMFDIDDFRFKLGLAEGEYAKMANFKVRVLDIALNQINELTDITASYEQHKVGRTISG
                     FSFTFTAKHQPKEVTHTKPKKLSDKQIKFFANKLAHYDPFASKKAAVGESYADLEKRLL
                     IELQDSEIVGKYAGVLKEIGLEV"
                     /name="repM"
     gene            8836..9465
                     /locus_tag="LO007_RS19855"
     CDS             8836..9465
                     /locus_tag="LO007_RS19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000585003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_188137507.1"
                     /translation="MIILIGSQKGGCGKSTIAINIAAYLAKYKKDIVLVDADPQQSSAN
                     WVKDRDETDLPKVHCVQRYGDIKNTLKDLDSRYDFVIVDVAGHDSKELRTAMLTAHYLV
                     VPFRPSQFDLDTLPHLSEVIDQAKSFNEDLKPFGLLTLAPTNPSVSEVQQANDYLADFP
                     LFTALSSVIHDRKVYRDVTAEGKGVVESNNPKAVEEFESFMKELLT"
                     /name="AAA family ATPase"
     gene            9462..9698
                     /locus_tag="LO007_RS19860"
     CDS             9462..9698
                     /locus_tag="LO007_RS19860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_188137508.1"
                     /translation="MIKKREIAKPSSPDMDAFINGANEQTTQKLDPKAPRKFKTHTIPM
                     NEYEYQLLTELAEKYGQTHNGIIRFALKKLSEK"
                     /name="hypothetical protein"
     gene            complement(9975..10301)
                     /locus_tag="LO007_RS19865"
     CDS             complement(9975..10301)
                     /locus_tag="LO007_RS19865"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_188137509.1"
                     /translation="MSSKKENLPYQTTVIEVTETSIIGAKSLSVKMDEHRYKQLRLNAL
                     EKGMTHREILTLIYMCGRGGRLLIHILVNQSLLFIDPSIMLFSFYRIRYRSYRAICKTL
                     TSNQ"
                     /name="hypothetical protein"
     gene            complement(10834..11460)
                     /locus_tag="LO007_RS19870"
     CDS             complement(10834..11460)
                     /locus_tag="LO007_RS19870"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_188137493.1"
                     /translation="MSNYFILLKDHFADTIQKSVKNSNLRLGRSHCFELIASFFGFNTY
                     AALMADGFLSTDRYQDEPTVDLKKFTERAQHLGISDQVTDIVKNCLLSLHGDEQDYLTS
                     LVKRLTRNIKRNCNLYYDYRGPIFDSHVFLNEEDWESCGDIAASDEFEDYLFTDLPLPS
                     LGTIVAVHNHPIDGLYQMNPDPILNILAFVAPSQIDQLLQEMRVR"
                     /name="hypothetical protein"
     gene            11884..12489
                     /locus_tag="LO007_RS19875"
     CDS             11884..12489
                     /locus_tag="LO007_RS19875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004733280.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_108564079.1"
                     /translation="MNTRIYLRASTKDQDAERALAELNALNNSLELGKPIIYVEHYSGT
                     KLERPELNKLLQEANQGDALLVESVDRLSRLTQHDFNELKRRIQEKGLRLIVADLPTTH
                     HLIQVNDNITSSILGLINNMLIDLLATMARLDNEKRIERIKQGLERSGYKPTGKKPNQT
                     KHKRIKELLASNTMTKEEIAKAVGCGVATVYRVAKRVN"
                     /name="recombinase family protein"
     gene            12559..12990
                     /locus_tag="LO007_RS19880"
     CDS             12559..12990
                     /locus_tag="LO007_RS19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_188137492.1"
                     /translation="MNSDAIWTETEESRLQREDEVRMQELKRLFNLMDEQELEQKTLRV
                     KLERALRILSWDDFEDIEVCPGLAELKGQQPIDTIKQHRKWLKALRSAMPYLAKILLAT
                     DASLLIRLRSTLDRYAEISYPNLLVMIQPKLQTLNSIKA"
                     /name="hypothetical protein"
     gene            12993..13241
                     /locus_tag="LO007_RS19885"
     CDS             12993..13241
                     /locus_tag="LO007_RS19885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002057569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="WP_104488392.1"
                     /translation="MTQLIHARMTASITELKKSPMDTVLAGKGEPVAILNRNTPAFYCV
                     PADVYETMMEQLEDIELNKIADARANQKRIRVNIDDL"
                     /name="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            13231..13518
                     /locus_tag="LO007_RS19890"
     CDS             13231..13518
                     /locus_tag="LO007_RS19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005021147.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_188137491.1"
                     /translation="MTYKLDFLEEALEEWNKLNPSIKQPLKKKLIKVLENPRIPKNKLS
                     GHPNRYKIKLRSIGYRLVYEVIDDEVVVIVIAVGRRENNAVYDDANSRHS"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
ORIGIN
        1 ggctttacta ggttattgct tggactatcc tttaaatcaa accagcaaaa tcacttgatt
       61 atttatacaa aaaatctatc aattatctac cttctcaaag ccatattgga tagtacttgc
      121 cccccttctc tgatctgcca cttacgaaca tgtgcataat tggttaacat gccattcaac
      181 taaaatcata taagctattc attaaaggac gattcatggc tcgtgctcca aagaaagaaa
      241 ccaaacaaga acccttagag gttgtgttat ggaagacagc agacaaactt cgcaagaaca
      301 ttgatgctgc tgagtacaag catgttgttc ttggtttgat cttcttgaag tacatctctg
      361 actcatttga agcccactac aacttactgg ttttaggtga aggagagttt gcaggagctg
      421 accctgaaga ccgtgatgaa tacactgcat acaacgtctt ctttgttcct gagaatgcac
      481 gttggtcatt cctgttaaac caagccaaac aacctaacat tggcaagctc gtcgatgatg
      541 caatggaagc catcgagaat gacaaccctc agttgaaggg cgttctgcct aaggtctatg
      601 cacgtcagaa tctcgatcct acgagcctag gtgagctaat tgacctgatt ggtaacattg
      661 cactaggcga tgcgaaatcg cgctctgctg acgttctagg gcacgtattt gagtacttcc
      721 tgggtgagtt cgctttagct gaaggtaagc aaggcgggca gttctacacg cctaaatcca
      781 ttgttagtct gttggtcaat atgcttgagc catacaaagg acgtgtattc gatccttgct
      841 gtggttcggg cggtatgttt gtacagtcag agaagttcgt ggaatcccat cagggcttgg
      901 tagatgacat ctccatctat ggtcaagaaa gtaaccagac cacttggcgt ttggccaaga
      961 tgaacctggc aattcgtggc attaattcag agcatgtgaa atggaactct gagggttcat
     1021 tcctcaatga tgcacacaaa gaccttaaag cggattacat cattgcgaat ccaccattca
     1081 acgtctcgga ctggagtggt gagcaattag taggtgatgc tcgctggcag cttggtacac
     1141 cacctgcagg taatgcgaac tttgcatgga tgcaacactt cttgtatcac atgagtccta
     1201 aaggccaggc aggtgtagtc cttgcgaaag gtgcgcttac ttctaagacc agtggtgagg
     1261 gtgagattcg taaagccttg gtttctgaag ccaatgtcat tgactgtatt gtgaacctac
     1321 ctgccaagct gttcttgaat actcagattc ctgctgcttt atggtttatg cgtcgtgatc
     1381 gtgtgaactc aactaagtac aaagatcgta gtaatgagat tctgttcatt gatgctcgta
     1441 acctaggtca tctcattaac cgcagaacca aagtgttatc tgatgaagac attaagctga
     1501 tcactgacac ttaccacaat tggcgtaaca aagatggtga gtatgaagat gtagcaggat
     1561 tctgtgcatc tgtaccgctt gagaaagtag ctgaacttga ttatgtactc acaccaggtc
     1621 gttatgttgg tttagctgat gacgaagatg agtttgactt taaggaacgt tttactagcc
     1681 tgaaagcaga gtttgaggca cagcttgcag aggaaaccaa gctcaatcaa gccattgctg
     1741 agaacttggc aaaggtgatt ctatgagtga gtggtcaatt actaaattag gcgatgtact
     1801 agagataaca aatggttatg cgtttaaatc agttaacttt agatcagagc ctgaagcagg
     1861 ttgtctacct gtccttaaga ttaagaatgt tgctaatggt gatgtacata taaacgatgt
     1921 tcaatatcat cattttgaag agaagctatc caaatacaca gctcaaaaag gagatattct
     1981 gatttctttg acaggaaatc atcctcatgc agagacacaa gttgttggtg ctgtttctaa
     2041 gcttaagatg gatatcagag cttttgtgaa tcagcgtgta gcgaagattg atactaagcc
     2101 taacttagct gataaagact acatttactt tgctcttaaa gaagaaggct tatcgaagta
     2161 tcttgcaagt cagtcatctg ggagtgcaaa ccaagcaaat atttctaagg ctgatattga
     2221 agatctagat atcgctttac cacctctccc tgaacaaaaa actatagcct cagtcctatc
     2281 atcattagat gacaagattg acttactaca tcgtcagaac aagaccttgg aagccatggc
     2341 tgaaacactg tttcgtcaat ggtttattga ggaggcgaaa gaggattggg agatattcca
     2401 attacaagac ttggcaaagc tacaaatagg aagaactcca cctagaaaag aaagtcattg
     2461 gttttctgat aatactaatg atatgaaatg ggtgtctatt aaagacttag gtgctcatgg
     2521 tatttttgtg acttattcaa gtgaatactt acctatatca actatagacc atttcaatat
     2581 tccaattatt cctgaaggga ctgtaatgtt gagctttaag atgacagtcg gacgggttgg
     2641 aatagcaatt gagaatatgg tatcaaatga ggcaattgca caattccaaa tcaaaaagcg
     2701 tttaacaagt gaattacttt actgcttctt gaagcaattc aattttgata cattaggttc
     2761 aacctcatct atcgttactg ctgtaaacac agctcttctg aaaggaattg aaattgcttt
     2821 gccagaggat gatcttattc aggagtttac agaacaggtt aagtcttatt ttgataaggt
     2881 caaagcaaat caactacaaa tccaaacact agaaaagcta agagacacgc ttctaccaaa
     2941 gctcatgagt ggtgaagtga gagtccaata ccaaacagaa gaagttgcat aacattgagg
     3001 tgaggtcatt tgatggcttc acctttttta cacccagggg gaaacatgaa caagaagctg
     3061 ataggggctt taatcactgt tattgtcgtg ctagttgggg gctattatta tgcttcgcca
     3121 tacattgtac tcaatagcat taagagtgca gcacaagcag gtgatagcga gaaggtatcc
     3181 gcatacatag actattcaag tgtgcgtcaa agcttcaaag atcaaatgaa tgcttacatg
     3241 gttaaagacc tagcatctaa agacacagat ggttgggaag cattgggaac aatgatagca
     3301 acgactatga tcgataaaat ggtagacgct gtcgttacgc cggaaggtat gacattgatg
     3361 cttcaaggga aagactttaa ggaaagttta aaaccacata cagagcaatc atcagatcag
     3421 actacttcaa aagaaaagtt ggattacagt acccgatatt tgtcgatgaa cttgttcgag
     3481 gtaacgttaa aaaatccaaa taatgacaag cagctaaaag taatcatgga gcgtgatggc
     3541 ttaagctgga aagtgaaaaa gtttgtcatt ccaatggatg atatggaaac caaccaaaca
     3601 acagaacagc aaccaacctt acctgtagaa gaaatactag cagaaactga ggcacctacc
     3661 attattgagc agccagagac agtatttgat tactctggtg tacagaaagg tgcacagctt
     3721 gagtcttgtt atcacgaccc ttgttctatc gctaaagtca tggggtttaa gctaataaac
     3781 caaacaccaa ctgaatcaga tctagaactc actgtagtgg gtggaagtag agggtttgag
     3841 tctgaatcta ttgagtggaa tcacgaagct cacaaaatcc agattcactg ttctattact
     3901 aaaccaacat tacagattgg ggatcaggta acggtaattc ctttaaatga ctctggtgtg
     3961 ccaggcgtgt tgtggtcaga tgccgaaacc tatatgcaag catgccacaa ctacactgga
     4021 gaaatgaggg caggcattca acagtatggc tataacgtgc agagttatga gtaaggttca
     4081 tagctttaca tcagataaca attagagagt ccatgcttta tggacaaatt taggggggaa
     4141 gtattctagt gacgaagatg accgaatcag atatcgagtt gatggcgata gaaaagctag
     4201 aggggatggg ctatacctat gtgtatgggc ctgatattga atctaaggga agcaaccctt
     4261 tacgaagcta taagcaggtc atcttagaag ccaaagtgct agaagcatta caacgtctta
     4321 accttcattt gaatgaagac aaatgcattg aagcactcaa gcaaatcacc aatctcgtat
     4381 cccctgattt actcaccaat aaccaagcct ttcaccggct actgacagaa ggtgtaaaca
     4441 tcgaagtcag caaagatggc aatacccaag gtgaatatgc ttggctcatt gacttcaaca
     4501 atccaagcaa caatgaattc cttgttgtaa accaagtcac tataagagaa gaccgttggg
     4561 aacgaagacc tgacatcatc ctctatgtga atggtttacc tttagtggtt attgagctca
     4621 agaatgctac cgatgagaac gccactgtag aaggtgcata taagcagata caaacttacc
     4681 aaagccaaat ccctagcttg tttacataca acgccttcaa catcatctcc gatggtttag
     4741 aagcgaaagc agggacagtg tcagcagact tgagtcgtta catggcgtgg aagaccacca
     4801 atggtcaaac caaagccaaa aagacacaag cacagcttga agtcttaatt catggtttac
     4861 tcaatccagt caccttactg gacatgatca gacacttcat tgtgtttgaa tccaataaac
     4921 aagaagatgc caatggcttg atcaccatta aaaccatcaa aaagatggct gcatatcatc
     4981 agtactatgc agtgaatgca gcagtcctct ctactattcg tgcgtctgca gtgaactcgg
     5041 actcgaagtc tgcagaagtc gcaatgcagc atcaaggcag aagtaagctc gaacttgtgc
     5101 aacaacaagc agtaggtgac aagaaggctg gtgttgtttg gcatacacaa ggctcaggta
     5161 agtctctgtc catggttttc tatacaggta agattgtctt ggctttggat aatccaactg
     5221 ttgttgtgat tacagaccgt aatgatttgg atgatcagct ctttggaacc tttgctgcat
     5281 caagccaact gttaagacaa acacctaagc aagcggatag ccgagaagaa cttaaagagt
     5341 tactcaaggt gggttcaggt ggggtgatct tcaccactat ccagaagttc caacctgatg
     5401 atggtggcag tgtctatgag caactctcag accgaaccaa tattgtcgta atagcagacg
     5461 aagcacatcg ttctcagtat ggcttcaatg caaaagaggt cgatgtcaaa gacgagaatg
     5521 gcaaggttgt cggtaagcgg actgtctatg gttttgccaa gtacatgcgt gatgctttac
     5581 cgaatgccac ttaccttggc ttcacaggaa cacctattga gaagacagat gtcaacacgc
     5641 ctgcagtctt tggtaactat gtcgacatct atgacatctc acaagctgtt gaggatggtg
     5701 caaccgttcg gatcttctat gaaagtcgct tagctaagat tgcgataagt gatgaaggta
     5761 aagaactgat tgaggacttt gacgaccagt tcaccgaaga cgatctaaac cagacacaaa
     5821 aagaacgggc aaagtggtca agagtcgaag gactcattgg cagtaaagag cgtatcaaag
     5881 ccattgccaa agacatcgta gaacactttg agcagcgtct aaaagccaat gcaaaccaag
     5941 gtaaaggcat gattgtcagc atgtctcgta ggattgctgt agatctctac aacgagattg
     6001 tagcgatcaa accagaatgg catagtgatg acttgcacga tggtgtaatc aaggtcgtga
     6061 tgacctcatc tgctgctgat ggtgaagaaa ttgccaagca ccatacaacc aagcagcaac
     6121 gccaaatcct ggctaatcgt atgaaggaca acgatgacaa actgaagctc gtcattgtgc
     6181 gtgatatgtg gctgactggt ttcgatgcac cgagtatgca tacgctctac atcgacaaac
     6241 caatgaaagg tcataacctc atgcaagcga ttgcacgagt gaaccgtgtc tataaggaca
     6301 agattggtgg acttgttgtt gattaccttg gtatcgcatc tgacctgaaa gaagcacttt
     6361 cattttactc tgaagcaggt ggtaagggtg accctgcatt agtgcaagac gaagctgcaa
     6421 ccattctaca agagaagctc gaagtgattg atggcatcat gcatggcttc aattaccaag
     6481 aatacttcac agccaatact tctcgtaagc tcaacattat ccttgaagca gaagaacaca
     6541 tcttaggtgt agaccaagga gaaggtaaga ctcgcttcct gacagcagtt gcagcgatgt
     6601 cacaggcatt tgctttagca gtgcctcatc ctcatgcgat ggaagcagca ccagtggttg
     6661 ccttcttcca agcagtgaaa gcgcgacttg cgaagttcag tgatggctca ggcaacgtct
     6721 caggcatgag taatgctgaa ttggaagcca aggttaaaca aaccattgac caggcactgg
     6781 tgactgaaca ggtggtggac atctttgatg cagcaggaat ccagaagcca gacatctcca
     6841 ttctctctga tgagttcatg caggagatga aaggatacaa gcacaagaac attgcgcttg
     6901 aaaccctgaa gaaactactt agtgatgaaa tcaaggtaag agaacagcgc agcgttaccc
     6961 agggtaagaa gctgatggat atgctgagct ctgccattaa gggctatcag aacaaggtgc
     7021 tgactgcagc cgaagtaatt gatgaactca tcaagttagc taaggcaatc caagaatcag
     7081 acaaaatggc agaggaactc aacctaactg aatatgaata tgctttctat tcagcagttg
     7141 cagacaatga cagtgccaga gagttgatgc agaaggacat tctcagagag cttgcagtag
     7201 tcctcactga gtccattcgg aagaatgtca ctctggattg gaccatcaaa gaagctgcta
     7261 gagcgaagct gagagtcatt gtgaagcgtc tactcaagaa gtatggctat ccacctgata
     7321 tggctttact cgcaacagag actgtcttgg aacaggcaga gcagttggca gatgaattat
     7381 ctaaagctgc ttaatttttt acaatcaaat tgcaatcatt aaataactaa attagttgtt
     7441 atatacaact aatttagttg caatctacac ctcaagacct atctctttga gaacaccagc
     7501 atactttccg acaatctcgc tatcttgtaa ttcaatcaga agtcttttct caagatcagc
     7561 gtagctctcg cctactgcag ctttcttgct agcaaatgga tcataatgag ctagcttatt
     7621 cgcaaagaat ttaatttgct tatctgagag cttcttaggc ttcgtatgag tgacttcctt
     7681 aggttggtgc ttagcagtga atgtaaatga gaaaccactg atcgtacgcc ccactttatg
     7741 ttgttcgtaa gaagctgtga tatctgtcag ttcattgatt tggtttaaag ctatgtctaa
     7801 tactcgaacc ttaaagttcg ccatctttgc atattcacct tcagctaacc caagtttgaa
     7861 tcgaaagtca tcaatatcaa acataggtgt ctttccaaca ctacgccatt ggatcagtaa
     7921 ctcgtaaagg cgaatcgcat acttacttgt aagagagcta atttgcttaa gttcgtaagc
     7981 agtgaatgac ttttcgagct gggtaatcag tggaataacg tcaggcgcaa aagtgagttc
     8041 tacgatacct gcagaatcaa cataggctat tttggaaacc caacgtgatt tgacgatctt
     8101 ttcctgctga gttccctcat ggataccttt gtaactgaat ttgcgctcaa agaggttgct
     8161 agcagcgttt ttaagccctt cataggctgc atgcttatca acgttaaatt gcttcatgta
     8221 atgatgagca tggacctcta gcgtgctatt agcgtccaca ttcttgctta actcccgggc
     8281 ttggacaatt gccaataaaa tcaatcgctg ttctgttgta tccaaattat agctagcatt
     8341 gattaaagca ttatccttta caactaagtc atttttcact actcaggtct tcccctctaa
     8401 ttatgacatt aggatacatc aatgatgtac gtagtcacaa ggtgggaaaa cgtagactta
     8461 tgggtgggaa aacgtagact tatgggtggg aaaacgtaga cttatgggtg ggaaaacgta
     8521 gacttatggg tgggaaaacg tagacttatc agctctgaaa cgcttgctat ataaggctca
     8581 cagagggtct aaaaataaaa atattaaaaa tattaaaaat aaaaagagtt tttggttaaa
     8641 aatttaagat tttttcttgt tttacgagta tctatttaca gcgataaaga cgaatgaaag
     8701 agagatttcc atataggtgg gaaaacgtag actaatgcta aacatagatt gagttgagag
     8761 attttttacc cattatttac attaacctaa aatgtaacta tattggattc aaattagtta
     8821 caaattacaa gtaaaatgat cattttaatt ggaagccaaa aaggtggatg tggtaaatca
     8881 acaatagcca tcaacatagc agcatatctt gctaaatata agaaagacat agtgttagtt
     8941 gatgcagatc ctcagcaaag ttcagcaaac tgggttaaag acagagatga aactgatctg
     9001 ccaaaagttc attgtgttca acgctatggt gacatcaaga acacactgaa agacctagat
     9061 agccgttatg actttgtaat tgttgatgtg gctggacatg atagtaaaga gcttagaaca
     9121 gcaatgctaa cagctcatta ccttgtagta ccgtttagac catcacaatt tgatcttgat
     9181 actttacctc atctttcaga agtcattgat caagccaaat catttaatga agacttaaaa
     9241 ccatttggtt tactaacact agcaccgaca aatccatctg tgtctgaagt acaacaagca
     9301 aatgattacc tggctgattt cccattattt acagcactgt cttcagtaat ccatgacagg
     9361 aaggtttatc gtgatgtcac ggctgaaggt aaaggggttg ttgaatccaa taacccaaaa
     9421 gcagtagaag aatttgaatc atttatgaag gagcttttaa cgtgattaag aaaagagaaa
     9481 ttgcgaagcc ttcatcccct gacatggatg cattcatcaa tggtgctaat gaacagacca
     9541 cccaaaaact agatccgaaa gcacctagaa aatttaagac gcatacgatt ccaatgaacg
     9601 aatatgagta tcagttgctc actgagttgg ctgaaaagta tgggcaaacg cataatggaa
     9661 ttatcagatt tgctttgaag aaacttagtg aaaaatagaa actaatttag aaccaaatta
     9721 aaaccataat agtttcaagt tagctgtgct ttgaatgtaa aaaagccctt aatcattaag
     9781 ggtttttttg tatctgttag acctatattt tgtctagaat actgcgattg atgtgtctag
     9841 catagatctt ccatctggtg taaaacatca cagcttcttc cactaaatct tgacggctta
     9901 tatccagaaa ttccttgata gaattaagat gactatcaag ctcagggcta agatacacag
     9961 tagactttgt tcgcttactg gttggaagta agcgttttgc atatagctct atagctgcga
    10021 tatctgatcc tataaaaaga gaatagcatg atagatggat ctatgaacag gagcgactga
    10081 ttaaccaaaa tgtgaatgag caatctgcca cctcgtccac acatgtaaat caaagtcaat
    10141 atctccctat gagtcatccc tttctctaaa gcatttagcc taagctgttt ataacggtgt
    10201 tcatccattt ttacacttag gcttttagcc ccaataatgc tggtttctgt aacctcaatc
    10261 actgtggttt gataaggtaa attctctttt ttgctagaca tggtttgtcc tatacgtact
    10321 taagtaaagc gcgtaactat agtcaaaaga accacaatgt aaaggagtat ttttcaacaa
    10381 atatttttat atatcatata cttacttaag tacacacatg gtttttatca ttaaatttaa
    10441 gcaataaaat gctcattttt atgggattta tagctgtctc tgtgcaaatg aaagagtagg
    10501 attgcccgaa gaatttgctt tcatgcaagc agaacagtta tacgactgct ctgtgaattg
    10561 attcaattaa gcgtcaaagt aaaaagcccg cttaactaag cgggcttttt acaaataatg
    10621 gagctttaat cttcatcaat ttctatgaag ccatctcctc cacatcgggt acaatcctga
    10681 tcttctataa ctccatcacc acaacattct tcacactgac agattttatc tccatcaaag
    10741 gtctcaccac agccgttaca attgttgcat ctaacgtcta ttaccttgtt acctcggcac
    10801 ttaggacaca taacttccat aataatctcc ctgttatcga acccgcattt cttgtaaaag
    10861 ttgatcaatt tgacttggag ctacaaaagc taagatattc aagataggat caggattcat
    10921 ttgatacaaa ccatcaattg gatggttatg aactgctacg atagtaccta gactaggcaa
    10981 tggcagatcg gtaaatagat aatcttcaaa ctcatcactt gctgcaatgt cgccacaaga
    11041 ctcccaatct tcctcattta gaaatacgtg actatcgaat atagggcctc tatagtcgta
    11101 ataaagattg caattgcgtt ttatgttacg agttaacctt ttaactaacg aggttaagta
    11161 gtcctgttcg tcgccatgga gtgataataa gcagtttttt acaatgtcag ttacttgatc
    11221 agatattcca aggtgttggg ccctctcagt aaattttttc aaatcaactg ttggttcatc
    11281 ttgatatctg tctgttgaaa gaaaaccatc cgccattaat gcagcgtaag tattaaatcc
    11341 aaagaaagaa gctattaact caaagcagtg actacgaccg agacgtaaat tagagttttt
    11401 tactgatttt tgaattgtgt cagcgaagtg gtctttcagt aaaatgaaat agttagacat
    11461 gagaacatcc ttaaaattga attatttatg tgtgctttag tttttgtata ggtggtcttt
    11521 ttatgcaata caaatgaggc acacaattaa ctcgacaata aggcgcttca atttatgtta
    11581 gaccaaatat cgaccttcta ggaggttagc ttaaccactc gctggttacc aagtctttga
    11641 tctaaaaata ttctgccaaa taaataattt gatgtaaatg agtttcttaa aaaatttgat
    11701 aagaaagaga atttattatt tctcatgaaa gagaaatttc cactattcat aatagtcaat
    11761 catttgacta tacccgaatg agaaacagcg aaacggtaaa tagctactat cattagactt
    11821 aaaaccataa tcaaatgaca gtgttatcat aggacttgat aatccaagta caaatgataa
    11881 aacatgaata cacgaatcta tcttcgcgcc tctaccaaag atcaagatgc agaaagggca
    11941 ttggctgaac tgaatgctct caacaactcg ttggaattgg ggaagccaat catctatgtt
    12001 gagcattaca gtggaacaaa gctagaacgg ccagagctga ataagttgct gcaggaagca
    12061 aaccaaggag atgcattact tgttgaaagt gtggaccggc tatcacgact aacccaacat
    12121 gatttcaatg aactcaaacg aagaatccaa gagaaaggcc tccgattaat tgtcgcggat
    12181 cttccgacca ctcatcacct gatccaagtc aatgacaata tcacaagctc tattctgggt
    12241 ctgatcaaca acatgctgat tgacctattg gctacgatgg ctcgcctgga taacgagaag
    12301 cgcatagagc ggattaagca aggccttgag cgttctggct acaaaccaac agggaagaaa
    12361 ccaaaccaga ccaaacataa gcgcatcaaa gagctgctgg cctcaaatac aatgaccaaa
    12421 gaggaaatcg ctaaagctgt gggctgtggt gtggcaactg tctatcgtgt agcaaagagg
    12481 gttaattaga gttttttaaa agcacgttta aatgtgctgt tatctgtgct atagtcctat
    12541 tattgaatag gagtaccaat gaattctgat gctatctgga ctgaaactga agaatctcgt
    12601 ttacaacgag aggatgaagt tcgtatgcag gaactcaaac gtctatttaa tttgatggac
    12661 gaacaggagt tggagcagaa gactctaaga gtaaagttag agagagctct gagaatactc
    12721 tcatgggatg actttgagga tattgaagta tgtcctgggt tagctgagct aaagggccag
    12781 caacctatag ataccatcaa acaacatcgt aaatggttaa aggcacttcg ttcagcgatg
    12841 ccttaccttg cgaagatact gctagctact gatgcctcct tgcttatcag attgagatcc
    12901 acattggatc ggtatgctga aattagctat cccaacctac ttgtaatgat tcaacccaaa
    12961 ctccaaacac taaattctat aaaggcctga ctatgactca attaatccat gcacgaatga
    13021 ctgcaagtat tactgaactc aagaagagtc cgatggatac agtgttagca ggtaaaggtg
    13081 aacctgttgc aatcctcaat cgtaatacac ctgcgtttta ctgtgttcct gccgatgtct
    13141 atgaaaccat gatggaacag ctagaagaca ttgaactcaa caaaattgca gatgctcgtg
    13201 cgaatcaaaa acgcattcga gtaaatattg atgacctata agttagactt tctagaagaa
    13261 gcattagagg aatggaacaa gcttaaccca agtattaagc agccattgaa gaagaagttg
    13321 atcaaggttt tggagaatcc tcgcattccc aaaaacaagc tgtcaggaca tcctaatcgc
    13381 tacaagatca aacttcgcag cattggttat cgtttggttt atgaagtcat tgatgatgaa
    13441 gtagttgtca tcgtaattgc tgtaggcaga cgtgaaaaca atgccgtcta tgatgatgcc
    13501 aactctcgtc attcataaat gatgtaatgc ctaaaggcta ctgcaaactt tcccgcatta
    13561 tcgccacctc actgcgttcg tggctcatgc ttcaagtttg cagtagcctt ctatgatgaa
    13621 aagttttgag aaaattgctc acattagtac gaagccaggt gagtggttct ttcaaccggt
    13681 accggggcat ctaccccccc tggggtcaaa tcagaaaagg attctttcaa gacagttttg
    13741 tatgcacttg tctcaatgct caaaccaatc catctatgcc accgagcaga tattggcaga
    13801 actatcaggc gactatagac tttgtgcaag gcttgtgaag gacactaagc ctcttctttg
    13861 ctacgtttca aacacctgct cattaataac ttttatgtat caatctatac cacaattgcc
    13921 ttgaactctc acataccaac taagtgatac ctaagtactg atgctattgg gtttgaacac
    13981 tatttaatca tttgatcttt gtttgcattt gaagaatttg acaatcctct tgacaagaaa
    14041 taggatctta ttagaaccat ataattacta atattcttat acataataaa tacttatata
    14101 ttatatactc tacttaataa gcaaattacc cccaaagaca taatatgtct ttgggggtaa
    14161 tttgtaccta gattctatat aaagaagaag tacattaatc acctacctta actttgttta
    14221 cccttctgca tactacgtac ttcgtactcc gtatttgaag ggtaaacaaa gttaagggaa
    14281 attaagggtt tataagatta gaaaagttga ataattacct caaatatctt ctagaacttg
    14341 tgtttttaag actctgctaa agcatgcagg tagacttcaa atcaaatatg cctgtaattc
    14401 ctcacagatt gcgctgtatt tgatttttca catcaccctg gtatgtttca ttacccaatc
    14461 caaaaaagtg aaaaatacgc gaaatggtga gcaattagag aatcatctca ttctcacact
    14521 caattgcgct gggtgttctt tgcct
//
