LOCUS       NZ_CP087296             6810 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Escherichia coli strain APEC E956 plasmid pE956_2, complete
            sequence.
ACCESSION   NZ_CP087296
VERSION     NZ_CP087296.1
DBLINK      BioSample: SAMN23175191
            Assembly: GCF_028752715.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6810)
  AUTHORS   Saliha,U., Tivendale,K.A., Noormohammadi,A.H. and Marenda,M.S.
  TITLE     Complete Genome sequence of avian pathogenic Escherichia coli field
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6810)
  AUTHORS   Saliha,U., Tivendale,K.A., Noormohammadi,A.H. and Marenda,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2021) Veterinary Sciences, University of
            Melbourne, 250, Princes Highway, Werribee, VIC 3030, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-NOV-2020
            Assembly Method                   :: unicycler v. version 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP006834
            Genome Coverage                   :: 55.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028752715.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 02:32:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,902
            CDSs (total)                      :: 4,785
            Genes (coding)                    :: 4,546
            CDSs (with protein)               :: 4,546
            Genes (RNA)                       :: 117
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 239
            CDSs (without protein)            :: 239
            Pseudo Genes (ambiguous residues) :: 0 of 239
            Pseudo Genes (frameshifted)       :: 108 of 239
            Pseudo Genes (incomplete)         :: 142 of 239
            Pseudo Genes (internal stop)      :: 53 of 239
            Pseudo Genes (multiple problems)  :: 58 of 239
            Pseudo Genes (short protein)      :: 4 of 239
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087296.1.
            Bacteria and source DNA available from Avian pathogenic E.coli.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="APEC E956"
                     /isolation_source="Tissue"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="pE956_2"
                     /geo_loc_name="Australia"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="1998"
                     /collected_by="University of Melbourne"
     gene            complement(join(5421..6810,1..374))
                     /locus_tag="LO740_RS24430"
                     /old_locus_tag="LO740_24375"
     CDS             complement(join(5421..6810,1..374))
                     /locus_tag="LO740_RS24430"
                     /old_locus_tag="LO740_24375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_274491478.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGSGIHWGGGSGRGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLA
                     VSISASELSAAIAGIIAKLKKVNLKFTPFGVVLSSLIPSEIAKDDPNMMSKIVTSLPAD
                     DITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFT
                     ASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGH
                     NAVYVSVSDVLSPEQVRQRQDEENRRQQEWNATHPVEVAEREYENARAELEAENKNVHS
                     LQVALDGLKNTAEGLALSDAGRHPLTSSESRFVAVPGYSGGGVHFDATATVDSRDRLNS
                     LLSLGGAAYVNNVLELGEVSAPTEDGLKVGNAIKNAMIEVYDKLRQRLITRQNEINHAQ
                     VSLNTAIESRNKKEEKKRSAENKLNEERNKPRKGTKDYGHDYHPAPETEEIKGLGDIKK
                     GIPKTPKQNGGGKRKRWIGDKGRKIYEWDSQHGELEGYRASDGQHLGSFDPKTGKQLKG
                     PDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(607..2826)
                     /gene="mobQ"
                     /locus_tag="LO740_RS24435"
                     /old_locus_tag="LO740_24380"
     CDS             complement(607..2826)
                     /gene="mobQ"
                     /locus_tag="LO740_RS24435"
                     /old_locus_tag="LO740_24380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_274491479.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRDSQLAREIELAIPREISREAARET
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKARPVASI
                     LSELPLMTWAEPEYRKRQLRFWKLEKQIESLLRTYRAVKERDIPARRQAFETQWNTWIS
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEYDARLKHHDNHRLSRETALAGVITGLG
                     RAREPGTGMITRYMMLSNRAGEFTVWGDELADYPQNVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDVTAYAAPEQLMLVVFSTERNSRTLNLLLCKGPEEVLVGFVRQEDGLRPV
                     LALPSPDYSHLIVSTITENGVCLAGYGEAINHDADTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            3014..3289
                     /locus_tag="LO740_RS24440"
                     /old_locus_tag="LO740_24385"
     CDS             3014..3289
                     /locus_tag="LO740_RS24440"
                     /old_locus_tag="LO740_24385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_039023505.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADMNRLQPLINELEKLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            complement(3496..4431)
                     /locus_tag="LO740_RS24445"
                     /old_locus_tag="LO740_24390"
     CDS             complement(3496..4431)
                     /locus_tag="LO740_RS24445"
                     /old_locus_tag="LO740_24390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011666805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_274491477.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSVKA
                     LKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRNFTPETFAQYVATTHTPEIQAARGRKGGIAKGAGYEDKRSTAL
                     EMLVNGCTQKAIAEMLGVSDRTIRNWKSGK"
                     /name="replication initiation protein"
     gene            complement(4927..5070)
                     /locus_tag="LO740_RS24450"
                     /old_locus_tag="LO740_24395"
     CDS             complement(4927..5070)
                     /locus_tag="LO740_RS24450"
                     /old_locus_tag="LO740_24395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001354641.1"
                     /translation="MKKITGIILLLLAVIILAACQANYIRDVQGGTVSPSSSAELTGLE
                     TQ"
                     /name="colicin release lysis protein"
     gene            complement(5154..5411)
                     /locus_tag="LO740_RS24455"
                     /old_locus_tag="LO740_24400"
     CDS             complement(5154..5411)
                     /locus_tag="LO740_RS24455"
                     /old_locus_tag="LO740_24400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032353782.1"
                     /translation="MGLKLDLTWFDKKSEEFKGEEYSKDFGDDGSIIERLGMPLKDNIN
                     NGCFDVKNEWVPLLQPYFKHQINLSDSSYFVSFDYRDGDW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             421..6810
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2469..2827
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.889"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          2828..3007
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="94.475"
                     /coverage="100.0"
                     /evalue="5.85e-73"
     Typing          complement(3497..4431)
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.142"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(611..2826)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.317"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ttttttaatt tagcaataat accagcaata gctgccgata attcgcttgc agaaatactg
       61 acagccagac cgccagctcc tggagtggaa agtgccggaa aaccaaatgc cactggcgca
      121 gctactgctg acagattacc gcctgttccc gaaccaccac cggaattgcc attacccccg
      181 ccattaccac gaccggagcc acctccccag tgaatgccgc taccggaacc accaccccac
      241 gggttatttt ccgaactcca tcctgaacca tcagaagcac caccacctac accaagcccg
      301 gtcgggccac cattaatgtt accacttgtg ctatgcgcgc ccgtgttatg gccgcgtcca
      361 tcgccaccgc tcataaaaat tccctctttt aagcgttaaa acaattaatt acacgattaa
      421 tactgtacat aaaaccactg gttttatgta cagatcaaaa agtacccgtc atcgtttttt
      481 ctgtcaagtt caggtggaaa attttttaga aatcaggagg gataacactt agaaaacgca
      541 gagcggacca caaaaaattt tgtggcccgt gatgtgttgg tagggttcgg ggggcagccc
      601 cggatatcac atactcatgc cccggaagtg cccggggcgg cgttgttgtt gcctgtgctg
      661 ttcgtcccgc ttccactcat gccaggtctg attaaaaccg agttgacgga agagataatc
      721 cggcatctct tccggtttgt ggacagcagc caaaagcctg tcatgatggc ctttgagccg
      781 gaactgcatc aggtgcggtt ccggtgggta cggagtatcc gcatcatggt ttatagcttc
      841 tccgtaacct gccaggcata ccccgttctc cgtgatggtg ctgactatca gatgactgta
      901 atccggcgac ggaagcgcaa gaacgggacg cagcccgtcc tcctggcgca caaatccgac
      961 aagcacttcc tccggccctt tgcacaacag cagattcagc gtcctgctgt ttcgctctgt
     1021 cgaaaataca acgagcatga gttgctccgg tgccgcataa gcggtgacgt ccacccggtg
     1081 cgcagacggc agcggagagg ctacgggcct gcggtcgtcg ctgaaataaa aattatccgg
     1141 ccagtgatgc ggcagcgccg gaaaaccgta agagggccac ccctggcgca tctgttccag
     1201 tacatgccgg accgtcgcac cggaatacat tctcacacgc tcaggccgcg ccatcgtctc
     1261 atgagattct ggcattccct cacgtatatc cgagaccatc acagccccgc ctggcgacag
     1321 gtaaacattc accgggtcat gaacattctg gggataatcc gccagttcat caccccatac
     1381 cgtgaattct ccggctctgt tactcagcat catgtagcgg gttatcatgc ccgttcccgg
     1441 ctctctggca cgccccagcc ccgtaataac cccggctaat gccgtttcac ggctcaggcg
     1501 atggttatca tggtgtttca gccgtgcgtc atattcctgt tccttccggc gcgcttccgc
     1561 ttcggcttcc tccctgcgcc gctccgcttc ccgtgctgac agtttttctt tcagctctgc
     1621 cattcccggc gaaatccacg tattccactg cgtttcaaag gcctgacgac gggcgggaat
     1681 gtcccgttct ttcacggccc tgtaggtgcg gagaagagat tcaatctgtt tctcgagttt
     1741 ccagaagcgg agttgtcttt tgcggtattc cggttcagcc catgtcatca ggggaagctc
     1801 tgacaggatg gacgctaccg gacgggcttt tctgagtccg gtcatctctt tttcgagaat
     1861 atcccgctct ttcaccagcg cattcccctc acgtttgatg tcgtttgcct tcccgttcat
     1921 cgccacgaac tccggtgtca tctcccatgc gttcccgcat tcctgcggca gtgtggcaat
     1981 gaattcccgc agggtatccg ctgaggcatt cgcggcggga atggtcagat taagggcttc
     2041 cgctgcacga cgtgcggcat cgctgagttc ccgtttccgc acgttctcct gtacctggat
     2101 gactgccagc cttgtgcggg aaacctgtat ttccaggtta aggctgttaa tcagacggtt
     2161 ctgctcaccg cgttctgttt ccattccccg tttttccatc gcacaggcag cctttccgag
     2221 gtgaaggccg ggcgctttct ccagtccctg acgctcgtat gaacgatggt ctatctcttc
     2281 ctggtagccg gcgttcgcaa gcgctctgtt cgcatggtca gcccatgatg cgcgccacgt
     2341 ctcggcgagt gcccggtcgt tccagtccct gacctttcct gcaaatcccg tttccccgac
     2401 agctctcgtg gtcagcatga tgtgcgcatg gggattggtc cggtccatgt gatggaacgc
     2461 cacatcggca atcatgcccc gactgacaaa gttttcccgg acaaaagcga gaacggtctc
     2521 ccgtgcggcc tcgcgggaaa tctcacgggg aatggcaagt tcaatctccc ttgccagctg
     2581 ggagtcccgg cgctgttcgg ctttctcgac cgcattccag agaacggaac ggtcaccaca
     2641 ccaggacggt gcattcgcag gggtgagaat gaccgcatca tcaacgccgc gtttacgggt
     2701 gtaatcgtgg gttaatccgg tacggtcatc gggtatgaca gagccggaac ggtaggcagc
     2761 agaagcaacg gcactgtagc cgttttttcg cgaaatgatt ttcatgctga gatgataaat
     2821 cgccatttct gctgtaatgc ctttttgccg ttttctttca gccccgcagg gcgcacacaa
     2881 tcgaaacgaa gtgaagattg tataagtgcg cacttcgtgc acttcgtgat atgccgtgca
     2941 ttatgccagt tcggttttgc aactaccagt gacgcgctaa aatacgattt ttcaccgcgc
     3001 acggagtcac gaaatgagca gaacactcga acagaagatt gccgaagctg aggccagatt
     3061 acagcgcctg aaggcgaaga gccggagcct ggacacggcg caaaaagtca ttgtgggggc
     3121 tgccatgctg gcgagggtac ggaggccgga agaagcgcaa ctgcgagcgt tcctgctcca
     3181 gttcctgcga aaggaagtca caagacaggc cgatatgaac cggctacaac cgctgattaa
     3241 cgagctggag aagctaccca gaccacctgc gaaaccgcag aaccactaag ccccggcgcg
     3301 taagtgccac atgcaccacc agacgccttt gcgcttcctc gctcattcgc tcgctacgct
     3361 cggtcatgag actgcggcga gcgcttccac cgcaatcacg aggaaaccag cgccattgct
     3421 ggacggaacc ggaaaaacca caggaacacc aaaagctcaa aaaagcgccg aaaaggcgct
     3481 gttatctgat ataggctatt ttccgctttt ccagtttcga atagtgcgat cggaaacacc
     3541 taacatttca gctatagcct tttgagtaca cccattcacc aacatttcca gcgcggtgct
     3601 gcgcttatcc tcatagccag cccctttggc tataccacct ttacggcctc gggcagcttg
     3661 aatttccggc gtgtgcgttg tcgccacata ctgtgcgaaa gtttccggcg taaagttcct
     3721 gtgcgtgtat ttcgctatgc tcttgccaat agcccgacat tcagccggag aaagcggaac
     3781 gggaagtgat gcgttgtaca tttcgacacg ctggatcacg gcatcaagcc attgtgagaa
     3841 tacaggccag ccctgacgaa tcgccctgta agcccatttg cgcgtcattt cgaacaggtg
     3901 gcagtttcgc cccatcccgt aatgtttatc gatgctacga cgctctgagg cgctcaaatc
     3961 gagataatct gccagttcat cgagggtata ggcttcctcg cgccactcca tcacctgcca
     4021 ttccaggtgg aacggatttt tgcaaatcag tccgctgtag ttcacatcag cacaaagttt
     4081 ctcacacagc gaacgttcaa tcgcagcggc gtatttcagc gccttaaccg agctgtccgg
     4141 cgcggttctc acggcgatat tgagcgcgta gagcaggtga gcatgaccgt tcacagggtt
     4201 ttttacggtg atgttcggcg cgggagcgtt ccggtcactc cagtcaatcg ctgccccctc
     4261 gcggtcaacg tcaaaaacca gccagaactg cgcatgaggc tggttctgct gtatgtaccg
     4321 cgcaagaagc gcacgccctt tgccggagat gcgaagacca aaagcgagat catccgtgtg
     4381 atagggacgg tggggtaaat tttcgtcgaa gtattgaagc gcggcgctca tgtgttacga
     4441 ttccttccag gttatcgacc gacctcgccc ctgtatttca tgttgaatct tgccggatct
     4501 ggacatgatt tcaggggcgt ttgtttttca gtcgcttaca ctacacccaa cggcaaatcc
     4561 tgtcgatcct tcccgcaaac ccgatccatt taccataact cctgttgtgc gcacccctta
     4621 aaacgctctc agacgcgttc tgacgcaaaa aacgcaaaaa gacaaggaaa caccaggctg
     4681 aaccagaaaa atgcgtcaga atcgttttta gggcgctgca taactatgca cgaaagcgat
     4741 gcgcgatcgg tttcgaccgt tccgaatggc gaacaggtca aaatgacaag ttttctccca
     4801 ccggatggaa ggttctgcac agggatttta gatcccatct cccgcatgga atttttgtga
     4861 catttcaaaa aaaagcgcat cagaatcagc ctgatacgct ttgttcttgt caaagaggat
     4921 ttcgggttac tgcgtttcta atccggtcag ttcagctgag gatgacgggg atacagtccc
     4981 gccctgaaca tcccggatat agtttgcctg acatgcagca agaataatga ctgcaagaag
     5041 caataaaata atccctgtta tttttttcat actgttcctt gtatatatgg attcaggaat
     5101 tcatccttga acacctgaat caaacacttt attactcgtc cctgatagtt taattaccag
     5161 tctccatcac gataatcaaa agaaacaaaa tacgagctat ccgaaagatt aatttgatgt
     5221 ttaaagtatg gttgcaatag aggaacccat tcattcttca catcgaaaca accattatta
     5281 atattatctt ttaaaggcat tccaagacgt tcaatgatag aaccatcatc accgaaatct
     5341 tttgaatatt cttccccctt aaactcttca cttttcttat caaaccaagt caaatctaat
     5401 ttaagcccca taacattccc ttaaagatat tttttaatat ttcgttttgg atctggacct
     5461 ttcaactgct tacctgtttt agggtcaaat gaaccaagat gctgaccatc actggctcga
     5521 tagccctcaa gctcaccatg ctgagagtcc cactcataaa ttttacgccc cttatcacca
     5581 atccagcgtt tgcgctttcc acctccattc tgttttggcg tctttggtat ccccttttta
     5641 atatcaccaa gcccttttat ttcttctgtt tccggagctg gatggtaatc atgaccataa
     5701 tctttagtgc cttttcgagg cttattcctt tcctcattta atttattttc cgcactcctt
     5761 ttcttttcct cttttttgtt acgactttca atcgctgtat taagagaaac ctgtgcgtgg
     5821 ttgatttcat tctgtcgggt aataaggcgc tgacgtaact tgtcatacac ctcaatcatt
     5881 gcatttttta ttgcattacc gacttttaaa ccatcctctg tcggggcact gacttcacct
     5941 aattcaagaa cgttattgac ataggccgca cccccaagac tcagaagact gttcagcctg
     6001 tcgcgcgaat caaccgttgc agtagcgtca aaatgtacac ctccaccact gtacccagga
     6061 acagcaacaa aacgggattc actggatgtt aacgggtgtc gtcctgcatc cgaaagcgca
     6121 agaccttccg cagtattttt cagtccgtca agcgcaacct gaagactatg aacgttcttg
     6181 ttctcagcct caagttctgc tcttgcattt tcatattcac gttctgccac ttccacagga
     6241 tgtgtggcat tccattcctg ctggcggcga ttttcttcat cctgacgttg tcttacctgc
     6301 tcaggactaa gaacatcact cactgaaaca tatacggcat tatgaccgct gtccttcggg
     6361 aatcgaataa ctgcatccct ggtattacca ccctgagtaa atcctgccgg gcgaacatcc
     6421 ttatcagtat tatttgtaac acctgggctt aatgtctgta ctgctggcgt actgttatta
     6481 actgaaatat tcagaacagg tgcacctgga attgatgccg taaaaacacc tggacgttcg
     6541 gtaggttttg catcaaccac cggaacactc atcggaacac ctgaaacaac cgaaatattc
     6601 tgtcgttcgt cttttacatc atcaacaaca cgaacattta cgtttactgt tgccttatcg
     6661 agaggtaatg aactgacagg tgattcagta atatcatctg cgggtaatga cgtcacaatc
     6721 tttgacatca tattggggtc atctttcgct atttccgacg gaattaatga agataagaca
     6781 accccaaaag gagtgaattt aagatttact
//
