LOCUS       NZ_CP087292             6584 bp    DNA     circular CON 27-MAR-2024
DEFINITION  Escherichia coli strain APEC 102026 plasmid p102026_2, complete
            sequence.
ACCESSION   NZ_CP087292
VERSION     NZ_CP087292.1
DBLINK      BioSample: SAMN23175190
            Assembly: GCF_028752695.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6584)
  AUTHORS   Saliha,U., Tivendale,K.A., Noormohammadi,A.H. and Marenda,M.S.
  TITLE     Complete Genome sequence of avian pathogenic Escherichia coli field
            isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6584)
  AUTHORS   Saliha,U., Tivendale,K.A., Noormohammadi,A.H. and Marenda,M.S.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-NOV-2021) Veterinary Sciences, University of
            Melbourne, 250, Princes Highway, Werribee, VIC 3030, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 30-NOV-2020
            Assembly Method                   :: unicycler v. version 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Reference-guided Assembly         :: CP006834
            Genome Coverage                   :: 55.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_028752695.1-RS_2024_03_27
            Annotation Date                   :: 03/27/2024 02:21:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,337
            CDSs (total)                      :: 5,218
            Genes (coding)                    :: 4,928
            CDSs (with protein)               :: 4,928
            Genes (RNA)                       :: 119
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 88
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 290
            CDSs (without protein)            :: 290
            Pseudo Genes (ambiguous residues) :: 0 of 290
            Pseudo Genes (frameshifted)       :: 132 of 290
            Pseudo Genes (incomplete)         :: 173 of 290
            Pseudo Genes (internal stop)      :: 61 of 290
            Pseudo Genes (multiple problems)  :: 66 of 290
            Pseudo Genes (short protein)      :: 4 of 290
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP087292.1.
            Bacteria and source DNA available from Avian pathogenic E.coli.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="APEC 102026"
                     /isolation_source="Tissue"
                     /host="chicken"
                     /db_xref="taxon:562"
                     /plasmid="p102026_2"
                     /geo_loc_name="Australia"
                     /lat_lon="37.8136 S 144.9631 E"
                     /collection_date="2019"
                     /collected_by="University of Melbourne"
     gene            210..368
                     /locus_tag="LO741_RS26745"
     CDS             210..368
                     /locus_tag="LO741_RS26745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            866..1093
                     /locus_tag="LO741_RS26575"
                     /old_locus_tag="LO741_26520"
     CDS             866..1093
                     /locus_tag="LO741_RS26575"
                     /old_locus_tag="LO741_26520"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_229471606.1"
                     /translation="MLYAIVISTILCPYSKYAIEYIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1090..2658)
                     /gene="cea"
                     /locus_tag="LO741_RS26580"
                     /old_locus_tag="LO741_26525"
     CDS             complement(1090..2658)
                     /gene="cea"
                     /locus_tag="LO741_RS26580"
                     /old_locus_tag="LO741_26525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_106489667.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGAPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2918..3355
                     /locus_tag="LO741_RS26585"
                     /old_locus_tag="LO741_26530"
     CDS             2918..3355
                     /locus_tag="LO741_RS26585"
                     /old_locus_tag="LO741_26530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3436..3855
                     /locus_tag="LO741_RS26590"
                     /old_locus_tag="LO741_26535"
     CDS             3436..3855
                     /locus_tag="LO741_RS26590"
                     /old_locus_tag="LO741_26535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4173..4406)
                     /locus_tag="LO741_RS26595"
                     /old_locus_tag="LO741_26540"
     CDS             complement(4173..4406)
                     /locus_tag="LO741_RS26595"
                     /old_locus_tag="LO741_26540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4413..4931)
                     /locus_tag="LO741_RS26600"
                     /old_locus_tag="LO741_26545"
     CDS             complement(4413..4931)
                     /locus_tag="LO741_RS26600"
                     /old_locus_tag="LO741_26545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4969..5667)
                     /locus_tag="LO741_RS26750"
                     /pseudo=""
     CDS             complement(<4969..5667)
                     /locus_tag="LO741_RS26750"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5609..5932)
                     /locus_tag="LO741_RS26610"
                     /old_locus_tag="LO741_26555"
     CDS             complement(5609..5932)
                     /locus_tag="LO741_RS26610"
                     /old_locus_tag="LO741_26555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5996..6187
                     /locus_tag="LO741_RS26615"
                     /old_locus_tag="LO741_26560"
     CDS             5996..6187
                     /locus_tag="LO741_RS26615"
                     /old_locus_tag="LO741_26560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          2..319
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.179"
                     /coverage="100.0"
                     /evalue="2.17e-150"
     Typing          6308..6584
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.19"
                     /coverage="100.0"
                     /evalue="8.92e-111"
     Typing          complement(4070..5619)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
       61 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      121 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg
      181 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      241 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aaggcacgcc ttaacgccac
      301 tggcagcagc cactggtaac cggattagca gagcgatgat ggcacaaacg gtgctacaga
      361 gttcttgaag tagtggcccg actacggcta cactagaagg acagtatttg gtatctgcgc
      421 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
      481 caccgttggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
      541 atctcaagaa gatcctttaa tcttttctac tgaaccgcga tccccgtcag tttagaagag
      601 gaggatggtg cgatggtccc tccctgaaca tcacgtatat agttagcctg acatccaaca
      661 aggaggttta tcgcgaatat tcccacaaaa aatcttttcc tcataactcg atccttataa
      721 aatgaaaaga atatatggcg aggtttaatt tatgagctta agatactaca taaaaaatat
      781 tttatttggc ctgtactgca cacttatata tatatacctt ataacaaaaa acagcgaagg
      841 gtattatttc cttgtgtcag ataagatgct atatgcaata gtgataagca ctattctatg
      901 tccatattca aaatatgcta ttgaatacat agcttttaac ttcataaaga aagatttttt
      961 cgagagaaga aaaaacctaa ataacgcccc cgtagcaaaa ttaaacctat ttatgctata
     1021 taatctactt tgtttggtcc tagcaatccc atttggattg ctaggacttt ttatatcaat
     1081 aaagaataat taaatcccta acacctcatt tatagtatta agtttattct tatcaatata
     1141 ggagcataga attcctgtaa caatagcaat accccaaata cctaatgtag ttccagcaag
     1201 caagctaaaa agtaaagcaa caacataact cacccctgca tctgcagctt tcttctctaa
     1261 tgtaagaaat agtggcttcc agtcacctgt atccttaatt tttaggatat cagataccac
     1321 atcatatcca aaagaaacat gccccgtaat cttcaagtac ttggcaaact gatctaaatg
     1381 tttagcccag tcatcatact tcaccgatgc caacgcatta aaaatagcat cacgatcggc
     1441 tttgctgaat ttcttattta aaacatcctt gtatttttca aaagcagcga gagcttcatt
     1501 cacattgccg attttcttac ctttagactt atcagcaagt tcctgtgcca ttttcgaata
     1561 tttttcacca tatttttcag tcagcgtttg ataaaagcta actgttgcat caacagcatc
     1621 cttaatctgt gaattaagga gattattctg tgcttttttc aaattttctt cagcttcatg
     1681 aacacgagcg ataccggcat tacgattatt actgacctga gaaatagcct tctggatctg
     1741 agttatatca gcatttatcc ggttaatacg tgtttctgat gctgttacct gtttttgttt
     1801 ttcttctcta atcttaccgg ccccaacccg tcgtctggtt gcttcaaaaa aaggacggtt
     1861 ctgaagcgga tcattggctc ttggtgatag ttttttgacc agctcatcca gttctttata
     1921 tttagcggat gcctgagcca gttcatttcg ttttccagcg agcgttttca tttctgcatc
     1981 acgggcatgg atactggagc ttaaacgaga attgagagtc ttaatctctc catccatttt
     2041 caccacttca gattgtgcag cagaaagttt tttttgggcg atctcaacag ctttagcttc
     2101 ttcactcaat gcagccagtc gtttctcttc agcttcagcc agtttcaact ggcgttctgt
     2161 ttcagccttc tcccgttcaa tctctttacg tcgttgttct gcttcctgaa aagccttttc
     2221 tgctgcttcc gcttctttac gggctttttc ttctgctttc gcaaggcgca aacgctctgc
     2281 ttccgcctgc atagctgcat tattagcatg agcaagctct gttgctgaag gcgtacgtga
     2341 ggcattgtga cgaagagcct cattcacgat atccttcagg cgctgagtca gcgcatccct
     2401 gtttgccttt gctttcgcct gtgcttccgc tgcagctttt gcccgggcag cctgctctgc
     2461 ctgtgttttc tttaattgag cagtagacca tttagcagtt gcatgaatag ctgcagaact
     2521 ttcactttta ctgcctcctt ttccacctcc gccgccagag ccactcccgt caggagcacc
     2581 attcaaaaga gtaataatta cctgtccctt atcatcataa ggaacaccat ctttatagta
     2641 cgctaccgcg gtttccatta taaaatcctc tttgactttt aaaacaataa gttaaaaata
     2701 aatactgtac atataaccac tggttttata tacagcataa aagctacgcc gctgcatttt
     2761 ccctgtcaag actgtggact tccatttttg tgaaaacgat caaaaaaaca gtctttcaca
     2821 ccacgcgcta ttctcgcccg atgccacaaa aaccagcaca aacattaccg ttctcagacc
     2881 tcattatgtt ttactgaaac tatgagatga gacatctatg ggacactgtc actttatggc
     2941 atggcacaca ctccgggacg cactaaaaat gacaggcaga tcgcgttcac agttttaccg
     3001 tgatatgcgc gcaggccttg tcagttaccg taccggcagg gacggacgac gggagtttga
     3061 aaccagtgaa ctgatccggg catacggcga attaaagcag aatgagacac cagaaaggca
     3121 cagtgaggga catgcagaaa atccacatga tcagcagaca gaacgcattc tccgggaact
     3181 gaatgagctg aaacaatgcc tgacgctgat gcttgaggat aaacaggcac aggatatgga
     3241 tcgcagacgc caggaagcag aacgggaaca gctacaaaat gagatagccc agctcaggca
     3301 ggcactggaa ctggaaaaga aacggggatt ctggtccagg ttgttcggtc gctgaacgct
     3361 gtcagagact gatgataaaa tagtcttcgg ataataactc accgagaata aatactttaa
     3421 ggtagggaga cactcatgag acgtaccgga aacaaacttt gtcttatcgc catgataaca
     3481 gcaacagtag ctctcacagc ctgtacccca aagggcagcg tggaacaaca tacccggcat
     3541 tacgtatatg cttctgatga cggttttgat cccaactttt ccacccaaaa agccgacaca
     3601 acacgaatga tggtgccttt ttttcggcag ttctgggata tgggagctaa agataaagcg
     3661 acaggaaaat cacggagtga tgtgcaacaa cgcattcagc agtttcacag ccaggaattt
     3721 ttaaactcac tccggggcac aactcaattt gcgggtactg attaccgcag caaagacctt
     3781 accccgaaaa aatccaggct gctggctgac acgatttctg cggtttatct cgatggctac
     3841 gagggcagac agtaagtgga tttaccataa tcccttaatt gtacgcaccg ctaaaacgcg
     3901 ttcagcgcga tcacggcagc agacaggtaa aaatggcaac aaaccacccg aaaaactgcc
     3961 gcgatcgcgc ctgataaatt ttaaccgtat gaatacctat gcaaccagag ggtacaggcc
     4021 acattacccc cacttaatcc actgaagctg ccatttttca tggtttcacc atcccagcga
     4081 agggccatcc agcgtgcgtt cctgtatttc cggctgacgc tcccgttcca gggataacac
     4141 atgttcgcgc tcctgtatca gccgttcctc tcttatctcc agttctcgct gtataactgg
     4201 ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg catgacccgc
     4261 tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca tcttctgcaa ttccacgaag
     4321 gcgctctccc attcgctcag ccgctgcata tagtcctgtt gcagctgctc taaggcgttc
     4381 agcaaatgtg tttccagctc tgtcactctg tgtcactcct tcagatgtac ccactctttc
     4441 ccctgaaagg gaatcacctc cgctgatttc ccgtacggaa ggacaaggaa tttcctgttc
     4501 ccgtcctgca caaactccac gccccatgtc ttcgcgttca gtttctgcaa tgtctcttcc
     4561 tgcttcctga tttcttccag gttcgcctgt atcctccctc caagatacca gagcgtcccg
     4621 ccactcgcgg taaacaggag aaagactatc cccagtaaca tcatgcccgt attccctgcc
     4681 agctttaaca cgtccctcct gtgctgcatc atcgcctctt tcaccccttc ccggtgtttt
     4741 tccagcgatt cctctgtcga ggctgtgaac agggctatag cgtctctgat tttcgtctcg
     4801 tttgatgtca cagcctcgct tacagattcg ccgagcctcc tgaactcgtt gttcagcatt
     4861 ttctctgtag attcggctct ctctttcagc tttttctcga actccgcgcc cgtctgcaaa
     4921 agattgctca taaaatgctc ctttcagcct gatattcttc ccgccgttcg gatctgcaat
     4981 gctgatactg cttcgcgtca ccctgaccac ttccagcccc gcctcagtga gcgcctgaat
     5041 cacatcctga cggcctttta tctctccggc atggtaaagt gcatctatac ctcgcgtgac
     5101 gccctcagca agcgcctgtt tcgtttcagg caggttatca gggagtgtca gcgtcctgcg
     5161 gttctccggg gcgttcgggt catgcagccc gtaatggtga tttaccagcg tctgccaggc
     5221 atcaattcta ggcctgtctg cgcggtcgta gtacggctgg aggcgttttc cggtctgtag
     5281 ctccatgttc ggaatgacaa aattcagctc aagccgtccc ttgtcctggt gctccaccca
     5341 caggatgctg tactgatttt tttcgagacc gggcatcagt acacgctcaa agctcgccat
     5401 cactttttca cgtcctcccg gcggcagctc cttctccgcg aacgacagaa caccggacgt
     5461 gtatttcttc gcaaatggcg tggcatcgat gagttcccgg acttcttccg gattaccctg
     5521 aagcaccgtt gcgccttcgc ggttacgctc cctccccagc aggtaatcaa ccggaccact
     5581 gccaccacct tttcccctgg catgaaattt aactatcatc ccgcgccccc tgttccctga
     5641 cagccagacg cagccggcgc agctcatccc cgatggccat cagtgcggcc accacctgaa
     5701 cccggtcacc ggaagaccac tgcccgctgt tcaccttacg ggctgtctga ttcaggttat
     5761 ttccgatggc ggccagctga cgcagtaacg gcggtgccag tgtcggcagt tttccggaac
     5821 gggcaaccgg ctcccccagg cagacccgcc gcatccatac cgccagttgt ttaccctcac
     5881 agcgttcaag taaccgggca tgttcatcat cagtaacccg tattgtgagc atcctctcgc
     5941 gtttcatcgg tatcattacc ccatgaacag aaatccccct tacacggagg catcagtgac
     6001 taaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct
     6061 gctggagaag ctcaacgaac tggacgcaga tgaacaggcc gatatttgtg aatcgcttca
     6121 cgaccacgcc gatgagcttt accgcagctg cctcgcacgt ttcggggatg acggtgaaaa
     6181 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtgagcgg atgccgggag
     6241 ctgacaagcc cgtcagggcg cgtcagcagg ttttagcggg tgtcggggcg cagccctgta
     6301 tgaaaagtac gccatgccgg gtgtgaaatg ccgcacagat gcgtaaggag aaaatgcacg
     6361 tccaggcgct tttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     6421 cgagcggtac tgactcacac aaaaacggta acacagttat ccacagaatc aggggataag
     6481 gccggaaaga acatgtgagc aaaagaccag gaacaggaag aaggccacgt agcaggcgtt
     6541 tttccatagg ctccgccccc ctgacgagca tcacaaaaat agac
//
