LOCUS       NZ_CP086575            20646 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Staphylococcus argenteus strain RIVM_M046968 plasmid
            pRIVM_M046968_1, complete sequence.
ACCESSION   NZ_CP086575
VERSION     NZ_CP086575.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22560078
            Assembly: GCF_023611825.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus argenteus
  ORGANISM  Staphylococcus argenteus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20646)
  AUTHORS   Witteveen,S., Hendrickx,A.P.A., de Haan,A., Landman,F., van
            Santen-Verheuvel,M.G. and Schouls,L.M.
  TITLE     Genetic characteristics of Staphylococcus argenteus isolates
            collected in the national Dutch MRSA surveillance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20646)
  AUTHORS   Witteveen,S., Hendrickx,A.P.A., de Haan,A., Landman,F., van
            Santen-Verheuvel,M.G. and Schouls,L.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Bacterial Surveillance and Response,
            National Institute for Public Health and the Environment, Antoni van
            Leeuwenhoeklaan 9, Bilthoven 3721 MA, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023611825.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:05:49
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,683
            CDSs (total)                      :: 2,601
            Genes (coding)                    :: 2,524
            CDSs (with protein)               :: 2,524
            Genes (RNA)                       :: 82
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 59
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 77
            CDSs (without protein)            :: 77
            Pseudo Genes (ambiguous residues) :: 0 of 77
            Pseudo Genes (frameshifted)       :: 40 of 77
            Pseudo Genes (incomplete)         :: 37 of 77
            Pseudo Genes (internal stop)      :: 18 of 77
            Pseudo Genes (multiple problems)  :: 16 of 77
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086575.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20646
                     /organism="Staphylococcus argenteus"
                     /mol_type="genomic DNA"
                     /strain="RIVM_M046968"
                     /host="Homo sapiens"
                     /db_xref="taxon:985002"
                     /plasmid="pRIVM_M046968_1"
                     /geo_loc_name="Netherlands"
                     /collection_date="2019"
     gene            166..842
                     /locus_tag="LK342_RS13260"
                     /old_locus_tag="LK342_13255"
                     /pseudo=""
     CDS             166..842
                     /locus_tag="LK342_RS13260"
                     /old_locus_tag="LK342_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <853..1135
                     /locus_tag="LK342_RS13265"
                     /old_locus_tag="LK342_13260"
                     /pseudo=""
     CDS             <853..1135
                     /locus_tag="LK342_RS13265"
                     /old_locus_tag="LK342_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2346..2963
                     /gene="cadD"
                     /locus_tag="LK342_RS13270"
                     /old_locus_tag="LK342_13265"
     ARG             2347..2960
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            2982..3329
                     /locus_tag="LK342_RS13275"
                     /old_locus_tag="LK342_13270"
     CDS             2982..3329
                     /locus_tag="LK342_RS13275"
                     /old_locus_tag="LK342_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3489..3581
                     /locus_tag="LK342_RS13280"
                     /old_locus_tag="LK342_13275"
     CDS             3489..3581
                     /locus_tag="LK342_RS13280"
                     /old_locus_tag="LK342_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3793..4767
                     /locus_tag="LK342_RS13285"
                     /old_locus_tag="LK342_13280"
     CDS             3793..4767
                     /locus_tag="LK342_RS13285"
                     /old_locus_tag="LK342_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072390.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4887..5702)
                     /locus_tag="LK342_RS13290"
                     /old_locus_tag="LK342_13285"
     CDS             complement(4887..5702)
                     /locus_tag="LK342_RS13290"
                     /old_locus_tag="LK342_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5695..7138)
                     /locus_tag="LK342_RS13295"
                     /old_locus_tag="LK342_13290"
                     /pseudo=""
     CDS             complement(5695..7138)
                     /locus_tag="LK342_RS13295"
                     /old_locus_tag="LK342_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /name="Mu transposase C-terminal domain-containing protein"
     gene            complement(7110..7703)
                     /locus_tag="LK342_RS13300"
                     /old_locus_tag="LK342_13295"
     CDS             complement(7110..7703)
                     /locus_tag="LK342_RS13300"
                     /old_locus_tag="LK342_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(7967..8347)
                     /gene="blaI"
                     /locus_tag="LK342_RS13305"
                     /old_locus_tag="LK342_13300"
     ARG             complement(7971..8347)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8337..10094)
                     /gene="blaR1"
                     /locus_tag="LK342_RS13310"
                     /old_locus_tag="LK342_13305"
     ARG             complement(8341..10094)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.56"
                     /coverage="100.0"
     gene            10201..11046
                     /gene="blaZ"
                     /locus_tag="LK342_RS13315"
                     /old_locus_tag="LK342_13310"
     ARG             10202..11043
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11415..11948
                     /locus_tag="LK342_RS13320"
                     /old_locus_tag="LK342_13315"
     CDS             11415..11948
                     /locus_tag="LK342_RS13320"
                     /old_locus_tag="LK342_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001823239.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNESK
                     RRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEVNIT
                     RQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            12362..12655
                     /locus_tag="LK342_RS13325"
                     /old_locus_tag="LK342_13320"
     CDS             12362..12655
                     /locus_tag="LK342_RS13325"
                     /old_locus_tag="LK342_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12730..14664
                     /locus_tag="LK342_RS13330"
                     /old_locus_tag="LK342_13325"
     CDS             12730..14664
                     /locus_tag="LK342_RS13330"
                     /old_locus_tag="LK342_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_250340856.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLDISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14668..14979
                     /locus_tag="LK342_RS13335"
                     /old_locus_tag="LK342_13330"
     CDS             14668..14979
                     /locus_tag="LK342_RS13335"
                     /old_locus_tag="LK342_13330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            14991..15617
                     /locus_tag="LK342_RS13340"
                     /old_locus_tag="LK342_13335"
     CDS             14991..15617
                     /locus_tag="LK342_RS13340"
                     /old_locus_tag="LK342_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15855..16484
                     /gene="mobV"
                     /locus_tag="LK342_RS13345"
                     /old_locus_tag="LK342_13340"
                     /pseudo=""
     CDS             15855..16484
                     /gene="mobV"
                     /locus_tag="LK342_RS13345"
                     /old_locus_tag="LK342_13340"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16578..17123
                     /locus_tag="LK342_RS13350"
                     /old_locus_tag="LK342_13345"
     CDS             16578..17123
                     /locus_tag="LK342_RS13350"
                     /old_locus_tag="LK342_13345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000273005.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            complement(17165..17449)
                     /locus_tag="LK342_RS13355"
                     /old_locus_tag="LK342_13350"
     CDS             complement(17165..17449)
                     /locus_tag="LK342_RS13355"
                     /old_locus_tag="LK342_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001793642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001793642.1"
                     /translation="MKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQKHSFYVA
                     KTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGATYFSS"
                     /name="hypothetical protein"
     gene            17507..18367
                     /locus_tag="LK342_RS13360"
                     /old_locus_tag="LK342_13355"
     CDS             17507..18367
                     /locus_tag="LK342_RS13360"
                     /old_locus_tag="LK342_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_031788340.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERENFLKQL
                     ELNWE"
                     /name="RepB family plasmid replication initiator protein"
     gene            18454..18795
                     /locus_tag="LK342_RS13365"
                     /old_locus_tag="LK342_13360"
     CDS             18454..18795
                     /locus_tag="LK342_RS13365"
                     /old_locus_tag="LK342_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19034..19105)
                     /locus_tag="LK342_RS13370"
                     /old_locus_tag="LK342_13365"
     CDS             complement(19034..19105)
                     /locus_tag="LK342_RS13370"
                     /old_locus_tag="LK342_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19254..19439
                     /locus_tag="LK342_RS13375"
                     /old_locus_tag="LK342_13370"
     CDS             19254..19439
                     /locus_tag="LK342_RS13375"
                     /old_locus_tag="LK342_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19658..20401)
                     /locus_tag="LK342_RS13380"
                     /old_locus_tag="LK342_13375"
     CDS             complement(19658..20401)
                     /locus_tag="LK342_RS13380"
                     /old_locus_tag="LK342_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843757.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEAKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10202..11046
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7362..17655
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1193..1340
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17508..18367
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.884"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19659..20401)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15856..16130
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ttggtaataa aacgtttaga aactgtttat ttatataatt ttcgatactt agattatata
       61 atgaataata tcttagtgca agcacctaga ttattaaata aacacaaaaa atgtaagcgt
      121 tttattacct cttaccaaac taaaattcgg aaagaggttt ttattatgtc aattaaactt
      181 tacaacataa atgaggaata cagagaaaag ttttatcaaa taccgaaagt attttttaca
      241 aatcctaaat atatcaagtt atccaatgat gcaaaaatgg catggggtat tcttagagat
      301 cgtttagatt tatcaattaa aaatggttgg gttgattcta aaacaggtaa tatttatttt
      361 tattacaaaa atgaaaaccc acaaaatata ttaaattgta aaaaagataa agtaattaaa
      421 ataaagaaag aacttaattg aagccgaatt actactacaa gaaagacaag gtttaaataa
      481 gacaaataaa ctttatttag ttaaaccaga agtaacagaa gatgatattt ataaaattca
      541 aaagcaagaa aacactgttg agagcagcaa tggcaaggaa gtcggaaaaa ccgacttcaa
      601 gaagtcgata aaacagaatc taatgatact gaacttagct atactgaatc taacaatact
      661 gaaataaatg atatgaatga cttgaatgat atagagtccg aaaatgaaat tagtaatcat
      721 tcaagtcaat gcaaggttat ttaatgcaaa caattttaac tgaatttgaa gaaacacatg
      781 cactttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa tcattcaaat
      841 gattttataa caatcaaaga tacatcaaaa tatttcgtta aatcatctga ctttttaact
      901 gatagcgatg aaaaaatcaa gaaattgtca gtgatctaga aaaagatttg tatcgaaaac
      961 gtatgttaag ttatggtgga ttgcttaaat aaaaacataa aattttaaac ttagacgatg
     1021 ctgaagatgg caatttaatt aatacaagtg atgaagataa aatagcaaat gaagaagaaa
     1081 aggcacattc gattacggca attttgaaaa gcaaaattat tatttaaggc attagtgata
     1141 actgatgtct ttttttatta cttttttgta aaaaaagtgc tgtcttattt ttgtgacaaa
     1201 tgctgtatgt agtgtcacaa aagtgtgaca cttgagcttt ttatgacccc acacaaaaac
     1261 attttcgaag ccttgaaata tctggctttg ccagatcccc cattcattga tggggacaaa
     1321 tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag tttttgataa
     1381 gaaaagccct gcaggaattt agaagaacgt ggggaagttt taaagaattt tatgctaatt
     1441 tttaatttgc atgtaaatgg gcagtgtcta aaaaatagtt attgtttttg ctcaaatttt
     1501 tgtttttgaa aatagcatat atttatttgg ctcatatttg cgttttaaaa gcgtatataa
     1561 gttttagata aaactatatg aatcaccctt aaatctttaa aatgccctct taaaattcaa
     1621 aataagggca taaaaaataa aagccccccc cagataaagt gaggtagctt tttctttcga
     1681 cgccgtatcg tcgatgtaat tcaaaaaagt tatgggctat aaatctacat cactttttca
     1741 agaatgtagt agcaatattc aacgcaaatt aatttttata actgctcgga atatctcaag
     1801 ccgtttcttt aattttgaaa taaaaaaatc gacgaaggtc gattacgttt ttgcacatgt
     1861 cgatttatcc gacgtataag tgcgccctta cgggatttaa ctagattata acgacgaatt
     1921 ttagacctgt aaagcaataa caattaggtc taccaggtag ataaagtgta caaagtagaa
     1981 ccgaaaaagt tatttatttt gaagattttt cgtagatata atttgctaat aaacttccta
     2041 aaactgaata aagaaaaata gttaaactca tgtaatcact ccataattct gaagtgtctc
     2101 catgtctacg catagaacac gcatttatgc cgagaaaatt tattgtgcgt tgagaagaac
     2161 ccttaactaa acttgcagac gaatgtcggc atagcgtgag ctattaagcc gaccattcga
     2221 caagttttgg gattgttaag ggttccgagg ctcaacgtca ataaagcaat tggaataaag
     2281 cacctatgat tatttaaaaa tataagttat aatacattca aaaatatttt tgaatgaggt
     2341 gtattatgat tcaaacggtt gttgctgctg ctgttcttta tattgctaca gcagttgatt
     2401 tattagtaat tttattaata ttttttgcta gagcaaagac tagaaaagaa tatagaaata
     2461 tttatgttgg tcaatattta gggtctatta ttttaatatt agttagtttg tttttagctt
     2521 ttgtattaaa ttatgttcca gaaaagtgga tattaggttt attgggttta ataccaattt
     2581 atcttggaat taaagtggct atttatgatg attgtgaagg agaaaagaga gctaaaaaag
     2641 aattgaatga aaaaggattg tctaaattag ttggtacggt tgcaattgtt acgatagcaa
     2701 gttgtggcgc cgataatatt ggtttatttg ttccttattt tgtgacatta agtgttacta
     2761 atttattact tactctgttt gtctttttaa ttttaatttt cttcttggtg tttactgcac
     2821 aaaaattagc taatatccca ggaattggag aaattgttga gaaatttagt cgttggatta
     2881 tggctatcat ttatatagct ttaggtttat ttattattat tgaaaatgac actattcaaa
     2941 caattttagg atttatattt taatttaggg tgtgatttta tatgagttat gaaaatactt
     3001 gtgatgtgat ctgtgtacat gaggataaag ttaacaatgc tttaagtttt ttagaagatg
     3061 ataaatctaa gaaattactt aacattttag aaaaaatttg tgatgagaag aaattgaaaa
     3121 tcatattatc tttgattaaa gaagatgagt tgtgtgtttg tgatatttct ttgatattga
     3181 aaatgagtgt tgcttcaact tcacatcatt taaggctttt atataaaaat gaggtacttg
     3241 atttttataa agatggaaag atggcatatt attttattaa agacgatgaa ataagagagt
     3301 ttttctctaa aaatcaggag ggtttttgaa acgagtttct tcttgtcttg atactataaa
     3361 aaacaaagtt atataacata agacgactta tcggaagtta tctatcatcg tatattcgga
     3421 gatgatataa tatacaggtc gcctatctct caggcgtcaa ttcagacgca gagaggaggt
     3481 gcattacttt gctaatcatt ttcgttcaca tcatagcacc agtcatcagt ggctgtgctg
     3541 ttgcgtatta tacttattgg cttagtaaac gcaacaaata attaataggc gactttataa
     3601 gccagcatcg aaaaaatccc cacactattg cagtagtgtg gggattggtg cattaattgt
     3661 taatcatttt cgttatctag attataacac tggttagtag aggaatgcaa aaagataata
     3721 ttttaagtag tctttttgga tattatcata tgatatattt atgtaaattg attttttatt
     3781 aaaggagcat atatgagtaa tactaaaatt aaatatcaaa tttttatttc atcaacttat
     3841 ttagatttaa tagaagagcg tcaagcagta gtagaagcag ttttaaacag taataatatt
     3901 ccagcaggta tggaactttt taactcaagt agtaaaagcc aatgggaaat aataacacaa
     3961 tggatcaatg aatcagatgc agtgatatta ttgttaggtt ctagttatgg aacaattgat
     4021 agtgaagaaa atattagcta tacagaaatg gagtacaatt ttgctaaatc tttaggtaaa
     4081 cctatatatg tttttgagat gaataaagca tctaccgaat ccgcggaaaa gtctaaaaaa
     4141 ttagatgagt ttaagacaaa agttaaagaa aatagactaa ttagtccaaa tattaatgat
     4201 atttcggaat taaaaagtaa agtatctcag actttaatgc aaagtgtacc tagtgaaatt
     4261 aaagaaaata gtggatggat aagagtacag gattgtggta aaaatgtaaa aaaattaaaa
     4321 aaattggatg ctatacaaag agatttagat acatatgatt ttttgaaaga agaactttat
     4381 aaaatattca tgtatgtaaa aactttttat ttccagcaca caccatttga tgatagttat
     4441 attgaagata tagaaaacta cctgctaaat tatgaaaata atccacataa atcattcatg
     4501 aaaataaaag tttatgatta taaaaacttt ttaaaacatt taaaaaacat attgatttta
     4561 attaaacaag gaaaaaagcc attagctcaa gccggtcatt ttaaattaag cttttacgaa
     4621 gaggatttaa aattgttaca aaaaaattat aaggctacgc tagtcgatat taaaggattg
     4681 aataaacatt tttataagga atgtgatagg tgcttaaaag catataatat ttttattgag
     4741 ggtataaata aacatttata tgaatgatat aagtacaccc taatcataca tatataattg
     4801 ttgtcacttt actcgatata tgagaatgac tttacctaat aaatgagaaa agcaacgcac
     4861 tatattaaaa tgcgttgctt tttctattat cgaataccta taactaaact atctctagct
     4921 gtttctacaa cttctgtact tattttatct aaaccattta tatccataat tctgtctatc
     4981 tgcgcaaata aacgatgaat cagcctaaaa ttcccttttg taattttaat aatcgtcgtt
     5041 attgcctcat aatcagtaaa gtcttcgagt tttaaatcaa atcctaagtc ttgccactta
     5101 tattctaata tatgatgcgt ctcatcttta cttagattat caaattcatg cgcaaaacct
     5161 atacgcgaat atagttgagg ataacgagat agttttttct ctatacccgg cataccaata
     5221 aatatcattg ctaaattatg ttcatcataa atagctctta attgctctaa atgttgcact
     5281 ttaaggcgat caatctcatc tactataatt aaatcaatat atttaaacat atcactataa
     5341 tgtttactat gttcatgtcc atacttattt acaacatgta atttttgacc caaatcaatg
     5401 attgcagtaa tactatataa atcattcctt aatttagttt gtttttctgc tggtgcatta
     5461 taaaatattt tatccaccga tagtatgtca tcggtcgttt tactagctaa atcttcccaa
     5521 cctctatggt taacttgttt ttcaatagta tcccaatttg tgtaatacct agacgataaa
     5581 gtttttccga caccaggttg accataacat ataccaatat actgatattt aatacaagca
     5641 tcacaaaatt cagcaaagcg tttatactct ttcgtttcaa tgaaattttg atttttagtc
     5701 attacgatat ctctttaatt ttttgacgtt tttagtcgtt tcttgaggtg aataaccaca
     5761 atttttttct tccttaatta attccgtggt acttggacta gcaatatttt gttttaagtg
     5821 ttttcttctc tgactacgag cgtgctgtat gtcttttata tctatggagt aatcagctat
     5881 ttctggacat atagcagtac aaagaaattc atctctataa aacacacgta tttcagccat
     5941 atcatttggg ttataacgaa tcagtacata ttcacctaca taagcttcta gattagtatt
     6001 actgtatcga aaaccttgaa aatgaatgcc atctgaatga acttttctag atttaggtat
     6061 ttccaacaat aataaatcaa gttgttctaa gctacttggc atattaggga aaaagtgatt
     6121 actattccaa tgactgatag gcgtggtatg aatagtacta tgttcttttt gattataatc
     6181 ttcaattaaa aagtaacgta atttttcttc aaaattgtga aaatttatta gattactagg
     6241 agtatcatta ttgtttatat atccaggcag ttgttctaag aaagtttgat taactgtctg
     6301 aaagaatcgt tctatttttc cacgaccacg aggaacacca actttcgaaa acatcaagtt
     6361 aattttcaaa tcaatagcga cttgttccat atgatgtgaa gtaaagtcac ttccatgatc
     6421 tgtgtagaat ttttcgggta taccacacac tggccaattg gtatcatttt tattccatat
     6481 tgcttggtgt agtgttaatg ctgtgttttg agcattgggg ggcatcaaaa cttaagaaat
     6541 agcctgcaat tgcacgacta tagtcatcca taataatagt tagccatggt ctattgatat
     6601 tacctttttg gtctaatata aagatatcta gcaaagtatg gtctgcttgc catatttcat
     6661 tgggtctcga ggattctcta atttgtatta aatcgtactt attttgatag tatttttcac
     6721 cctgatgaga gaaatcaata acagacttag gcatcgcttt aataacacta taaatttgtt
     6781 tataacttgg tatatcaaaa ttattttctt tacagtaatt agtgattttc ctatgaattg
     6841 tggctatact atttctttta ttcataagcg caaggcgttc aatttcatca attatctttg
     6901 gttctatttt acgtgttcct ttatcagctc ttgtcgcata gattagtcct ttaagtcctt
     6961 gttctttata tgacttattc tacctataaa gggtgcttaa cgcaatccct ttacttttag
     7021 aaatacttga taaagattgt tcgcctagga taaaaggttt tataatattg tacttttgag
     7081 tagctttttt acgtttggct tcagaaaaat tagttagata cttctccttg tttttcatct
     7141 tccttgtcct ccaatttatt gaggtaacga taaattgttg tacgtgaaac atgccactgt
     7201 tctgcaattg tcttaattgg cgtgccatta tcataaaggg tcttaagtaa gtttaaatct
     7261 ttttgattta atttttctgg tctgcctcca tatcggcctc tagcacgtgc tgcaattcgg
     7321 ccagctgaag aacgctctaa aattaaatta cgttcaaatt ctgcaaatgc tgcgaataaa
     7381 tgaaataata attgtccagt tgaacttgat ttatccattg ttatattttc ttctaaacta
     7441 tggaaactta cacctcgttc gttaagctca ttaactaatg tgattaaatc ctccatatta
     7501 cgtcccaatc tatctaatct ccaaacaaca atcgtatctc ctgacctcgc aaattcaatt
     7561 gctttatcta aaccaggtct tttacttttt gaaccactca tatggtcatt aaatatcttt
     7621 tcacaaccat atgtatttaa tcgatcctcc tgtaaattta aattttgtaa tccagttgaa
     7681 actcttgcat aacctatttt caaaatacaa acctcctata tacatcttca gtgtaccata
     7741 acttaattac aaggtctttt atgaacatag attttttgtt gggtttttga acacataatc
     7801 ctatgtaatt cttacttatt tagacataaa agaagagtgt acagaaaatg gttattttct
     7861 gtacactctc atctttaata atatatttta aatgtcatta acaaaactaa tttaataaga
     7921 gtcaagcata gtttacatat gtattatgtt tgaaacatac ctctaattac tttttactaa
     7981 tatcatttaa aatatctcgc aattcttcaa tttctttgtt atttaattct tcatttttcg
     8041 caaaattcaa cactaaactt ttcatatccc ctccatacag tttattaaga aaggttttag
     8101 cagttttcat tttaatatcg tcttctttaa tatttgatga gtaaaaataa atattttctg
     8161 atttgtatcg ttttataatc tcttttttat atagtcttgt gattaatgtt ctaatcgttt
     8221 tatcactaac ttctttatat ttttgaattt caactacaat ttcattagct gatactgatt
     8281 ttttatccca tattatattc ataacatccc attcagccat agatatttca acttgcttat
     8341 tggccattta aaacacccat tccttttaat attttttcac ttattaattc agcatttttc
     8401 ccagacgggt ttccatctga taaatgtgta gcaaaataat acttatcatg atttgtaatt
     8461 acgtaaccta caaaccaccc attattatac ttcccgttta ctatacctgt acctgttttc
     8521 ccatacagtt catacttttc atttttctta atcaataatg aagaagataa ttgattcttt
     8581 gcttttttac taaaatggtt attttgttcc atcatatttt taaaaactat tacttgttca
     8641 agattagata ttttcaaact atcttccatc caatagcttt tataacttcc caaattttca
     8701 ttaccataat ttaattgctt gagttgagcc gcagtatagt tctttggtat ttgattacta
     8761 atacgttcga agtaccaatt aactgaattt tgcattgctg tatttaaatc ttgttcctta
     8821 ttccaagact cgaaaagata atgtttatga ttccatgaca tacgtgaatt tttatcactt
     8881 ataatatgac ggtcaagtcc aaacagtgct aaataaattt tgtaagttga atcaggagaa
     8941 tatctttttc tactttcttt ttcattataa atatagtatt tgtctttctt catgctgtac
     9001 ataacgaaag atccactatt tgaaccaaaa tttttacttt catctaaaat ttggtaatcg
     9061 ctttgtaagg gctttttata attataatca gttaaggatt gccccattaa aaactggctt
     9121 tgaattatca tgataaaaaa agtaaaaata caaataaaaa ttagaattaa ttttgactgc
     9181 tttttcagat cggcttcttt tatattaatt aatctatttt tgagtaatga ttttttacca
     9241 ttaaatgaat ggcttaatat atttttgttg aaaaatgagg tttttaatac ggagtccata
     9301 atagattccg cataagtttt aaattcattt ttgttaatat tattaaggac aaatctatcg
     9361 gcttctactt cattgtcatg aattatcttt ctttttacaa tatgtactaa tggattgtaa
     9421 ctcataataa tagagaagat attaaagata attaaatgta aagtatctct atttttagca
     9481 tgagcatatt catgcagtat tatatatttt agtcttttat caattacact tttaaaatat
     9541 gaactaggaa tcaaaataat atatttccca taccaaaaag ttattggaga ttgaatagcc
     9601 tctgcttttc gaatcacaat atttttttta tattgatggt tgaaaagtat cgtatctatt
     9661 ttattttttt cattttcgtt tagataaagt gactgtttct ttaaatattt aagatataat
     9721 aaggctttca aaaatttaaa acttaaaata ataactaaaa ctatccaaat aactgtgcag
     9781 atattatcaa ttgaatccca attaaactta tggatatctg ttgcgaactc ttgaacaggt
     9841 ttggtggtaa ttatgttatg gttcaagtcg tgtgacttac tttcaactgt gggcgcttga
     9901 ttattcacat tattaaattt tataaaagag aatttaattg ggataaaagg aattaatcca
     9961 gcaagcacag ttagatacca aactttataa tttaaactat aattaaaata gcgttttaat
    10021 atgtacctaa aaaatactaa caatagaaat ataaaacaaa agcttactac actcattatt
    10081 agtaatttag ccattttgac accttctttc aaatatttat aataaacaat tgacatcaat
    10141 attacaattg taatattatt gttttgtaaa aattacaact gtaatatcgg agggtttatt
    10201 ttgaaaaagt taatattttt aattgtaatg gctttagttt taagtgcatg taattcaaac
    10261 agttcacatg ccaaagagtt aaatgattta gaaaaaaaat ataatgctca tattggtgtt
    10321 tatgctttag atactaaaag tggtaaggaa gtaaaattta attcagataa gagatttgcc
    10381 tatgcttcga cttcaaaagc gataaatagt gctattttgt tagaacaagt accttataat
    10441 aagttaaata aaaaagtaca tattaacaaa gatgatatag ttgcttattc tcctatttta
    10501 gaaaaatatg taggaaaaga catcacttta aaagaactta ttgaggcttc aatgacatat
    10561 agtgataata cagcaaacaa taaaattata aaagaaatcg ggggaatcaa aaaagttaaa
    10621 caacgtctaa aagaactagg agataaagta acaaatccag ttagatatga gatagaatta
    10681 aattactatt cgccaaagag caaaaaagat acttcaacgc ctgctgcttt cggcaagact
    10741 ttaaataaac ttatcgcaaa tggaaaatta agcaaaaaaa ataaaaattt cttacttgat
    10801 ttaatgttta ataataaaaa cggagacact ttaattaaag atggtgttcc aaaagactat
    10861 aaggttgctg ataaaagtgg tcaagcaata acatatgctt ctagaaatga tgttgctttt
    10921 gtttatccta agggccaatc tgaacctatt gttttagtca tttttacgaa taaagacaat
    10981 aaaagtgata agccaaatga taagttgata agtgaaaccg ccaagagtgt aatgaaggaa
    11041 ttttaatatt ctaaatgcat aataaatact gataacatct tatagtttgt attatatttt
    11101 ctattatcgt tgacatgtat aattttgata tcaaaacctg attttccctt tattattttc
    11161 gagatttatt ttcttaattc tctttaacaa actagaaata ttgtatatac aaaaaatcat
    11221 aaataataga tgaatagttt aattataggt gttcatcaat cgaaaaagca acgtatctta
    11281 tttaaagtgc gttgcttttt tctcatttat aaggttaaat aattctcata tatcaagcaa
    11341 agtgacataa ttgttgaatt tatagtaact gaaacgtatg attagggtgt atattaatta
    11401 tgagatggaa atcaatgatt gttggctatg cgagagtatc atcgaacgat caaaatttag
    11461 aaagacaact tgaaaattta aagacgtttt gtgctgaaaa aatatttaca gaaaaacagt
    11521 ctgggaaatc tattgaaaat agaccagtac ttcaagaagc acttaacttt ttgaggatgg
    11581 gagatcggtt tgtagtagaa tccattgatt gtttaggtcg taattatgat gaagtggttg
    11641 gcaatccatt actggataag tttatgaaag atttaattat tcaaatatta gcaatggttt
    11701 cagaacaaga gagaaatgaa agtaaacgtc gacaagcgca aggaattcaa gttgcgaagg
    11761 aaaaaggcgt atataaaggg cgccccttac tttattcacc aaatgctaaa gatccacaga
    11821 aacgtattat ttatcatcgc gttgtagaaa tgttagaaga aggccaatca attagtaaaa
    11881 ttgcgaaaga agtaaatata acaaggcaga cgatttacag aattaaaaac gataatgatt
    11941 tgatttaata aactttgttt caatgcaact gagagcttct cacagcgtct caaaacagat
    12001 gattataatc tattatgagt ttttaataat aagacaagaa aagagagcgt aaatatgctc
    12061 tctctttttc tcattaacga acgtttataa cgcaatcatg atgagagtat taatacagtt
    12121 aattatttga aatactttag tttatataat gttgaattat aggtagtgaa attgtattta
    12181 ttatacattg tcatatcgtt gtttaatgat agttaattta aggataatag aattaatatt
    12241 atatatagat gaacaaaaca agcaacagag cagaaaaaac acttttagat tatttttact
    12301 cattctctat tttatttatt tttgctataa taaataaaga atattaaatg gatgtgtttt
    12361 tatgaaaaag aaatttgttt ctagttgtat tgctagtaca atcttattcg gcactttatt
    12421 aggtgtaact tataaagctg aagctgcaac agtacatgta gctggtggtg tttggagcca
    12481 tggtattgga aaacattacg tatggtctta ttatagtcac aataaaagaa atcatggttc
    12541 aacagcagta gggaaatatt catcttttag tggtgttgct cgacctggtg ttcaatctaa
    12601 ggcatctgct ccaaaggctt ggggtggaaa caaaacattt tatagtcttc actaattaca
    12661 tatattaatt aaggagaata gacagaatta agtaaactta attctgtcta ttctcttgaa
    12721 agaggcaata tgaaaaaatt caaattattt ttagatttta gtactttatt attaatttca
    12781 gggctattat ttttattttt cttcaaagaa aatgaagaaa ttatacctga aagttccaat
    12841 attttaacaa tctctaattg ggataaatct aacagtaaat caaaagtgtt agatgtcatt
    12901 gaatcaggcg ccaaaaatca aaatatacaa ataattaaat ccgttaaaga ttttgataat
    12961 aaaaaagaat tttttgtttt caactcaaaa cgaaataatt ctgactttat acgtaataaa
    13021 acatcacttt taactccatc agatttatta aacagagaga taaaaggaaa gtattatatt
    13081 attggtgaac atttcaatgt agaagagctt acgagtgaat taaaaaaagc tggtttaaca
    13141 tctgaagttg aatacataaa tagaaacata ttattttttg aattagtcat agataatgat
    13201 ttattagtgc ctttcatatt cttaggcgta ttatatcttt tgtacttctt atatgacaga
    13261 ggatataatt tgaaatttta tgctattcaa aagctacatg gcttttcaac tacacgcatt
    13321 atttttcata atattcatat aaaaatcatt tattggctag tacttaccgc tattttcttt
    13381 attgctaata tattaatcat tcatatagcc aatttagaat tggatttctt aatgtttatt
    13441 cgtagattta tcgtgttgct ttttgttttt gcttgtatta ctacattgct gtggctgatt
    13501 tcgtatagtc ttcttttgaa gttaagtatt ccattaatct taaaagggaa aaaaggatat
    13561 aagttttcaa tatttatggg taccttcacc aaatgtatca tggtgctaat actatctttt
    13621 ttactggcac aaaatttaaa tgcttatacg aagttaaaga atatatatga ctctgaaaaa
    13681 atctggcaaa agttagataa ttactataca ttagatattt ctcctaatat tagaaattca
    13741 gaagaaaaac aaattttaga aactaatata tacaatttaa ttaagaaaag cgaacaactt
    13801 ggtggattgc ttttaaagaa taataatata gccaatcccg ataaaaacaa ttacattcct
    13861 gaaaatggca atgtcttttt tatcaataac aattttctga atttctataa aaatataaat
    13921 catgatttta aaatgattga caatcattcg gataaaataa acgtgtttat tcctccaagg
    13981 ttacaatatc aaaaatctga aattcaaaaa aatcaccaag gatggattga ctttcaaaag
    14041 caacaaaaca aaaaagtaga tgtgcaagta ctgtcaaaaa acgtatctgt tttttctttt
    14101 gatcgcacaa ctaatttgaa atttgggtat ctagattctc caatagtaat gttgttgaca
    14161 cttgacgatt taacaggaga tttttattta gctgctgttt ctcaaggtgg atatctattt
    14221 aaagatttag atacacttaa acagttgctt aaagataatc acctagaaga agaaattagc
    14281 ggtataacta attacaagga ttctgtgtta aatgaattaa atgaaacgaa aacaaaaatg
    14341 ataataacaa tagttactat tattattaac gttttcattc ttttgatagc tactgtattt
    14401 gaaacaattc aatattttag ctggaataaa aagcaactat tattaagaaa aattcatggt
    14461 tattcattat tcagcagtaa tgtacgttat ttaacaattt ctatcttact ttctattatg
    14521 ttagcttaca ctacgcatat tttatttggt tcaaagatat tactattcat aataatgtct
    14581 attgctatta ttcaacattt acttcaaatt gcttatatta aatatcttga aaaatacttt
    14641 aaagatttaa tgagggagat ttaaacaatg aaaagaactt taattattgt aacaattgta
    14701 ctattaatca tcatacttag tctgttattg gttagaaatg agcatttaga tcgttttaac
    14761 ccactgttaa aagaaaaagt atcttatgcc aaagttccaa aagataccca ggactataga
    14821 aatataactg tatactctaa aaatggagaa aaaaagagtt ataaattgga ttttttaggt
    14881 tatgatccta caaaagaata tgtgaaagta aatcataaag ggaaatatgt gcgttcgata
    14941 gaatacatta caaaagatat tcctagcttt ttaaaatgag gtgaccgata gtgataaaac
    15001 ttgaaaatgt ttttgtaaaa aaaggtaata aaaacatttt agacggatgt aactttaatt
    15061 ttgaaaaagg aaaatcatat gctttagttg gagaaagtgg cgctggaaaa tctactttac
    15121 ttaacataat cgctgggttc gaagatgtat cacaaggaag catatatatt gaagataagt
    15181 tactaaaaaa gaaagtcgat ttttatagat atacgttagg gtatttgttt caaaattttg
    15241 ctttacttga aaatcaaaca atcagtcaaa atttagattt agccctaaag ttcaaaaaga
    15301 ataaaaaaga taatatgaac ttaaagaaag aagtattaaa aaaggtcggc ctagatttag
    15361 atatcaagag aattgttagt tctctaagcg gtggtgaaca acaaagagta gcattggcaa
    15421 gattaatatt aaaagatccg aaaatcattt tagctgatga acctactggg tcactagata
    15481 cgaaaaatgg aaaaatagtt attgatttat tattgaagct acttgatgaa aataaaacta
    15541 tgattgtcgt aacgcatgat ttagagcttg cgaaacgatt tgatgtcata gtcaatattt
    15601 ctgagcttag aagttaagaa atattgatag gtatagccat acatagagaa aaaacaatat
    15661 gaaatatgat tagtcattac ttttgagttt tttaatttaa ggactggatt ttccagtcct
    15721 tttttatgat gacaaaaaac aagcctcgca gagcacacgt ttttaatgac ttatttggtt
    15781 tttaataagt ctagtatgtt aaaacttttc tgtgtttagg tgttataatc atatgcagtg
    15841 gaagttgatt tattatgtct tattcaatta ttcgagttgt taaagtgaaa tcaaaaacaa
    15901 atacaagagg tattcaaaga catattcaaa gagaaaataa aaattatgaa aatatagata
    15961 ttgatttaag taagtcatat ttaaactatg atttagtcaa tgatactaag tttgatttta
    16021 ataaaaaaat tgatgagaaa atcgaaaaaa attataaagg gaaacgcaaa attagaacag
    16081 atgccattaa acatattgat ggattaatta catcagataa tgtgtttttt aatcaattat
    16141 ctgaagaaga gacaaaatag ttttttaagc atgctaaagc gttcttggaa gaagaatacg
    16201 gtaaagaaaa tcttttatat gcgacagtac atatggatga gataacacca catatgcatt
    16261 atggcgtagt tcctattact aaagatggac gtttaagtgc gaaagaagtt gtaggtaata
    16321 aaaaagcatt aaccgaattc caagatagat tcaatacgta tattaataag caaggctatg
    16381 atttaaaacg aggaatttct agacaactta caaaagaaaa acatgatcaa gttagccgtt
    16441 ataaacaaaa aacagaatat cataaacaaa tgcatatgcg ctagaagcaa atagaggatt
    16501 atttaaagca agaaaatgat aaattgatac aaaactacca aaaatcatta gatacgctta
    16561 aaaagcttct gagtgtcatg tatgaaatta aaactaaaaa tgtaggtgga tggtttcata
    16621 aagagaagca agaaacaggc aatattgtaa ttactaaaac tgattttgaa aaatacacta
    16681 aacaaattaa agcagcacag atgatattag atgattatga atgtattaaa agtgggaagt
    16741 cattaaaaaa agcagagaaa caaaatgaaa gtttagttaa tgaattgact agtgtccata
    16801 tggaaaatga aaagctggtt gaagaattta atgatttagc acaacgttat aattatttgt
    16861 tgtcagagaa tgaaaagaaa gataaagaac taaattatac gcttaagtta ttcaatcaag
    16921 tttttaaaat cattaaaagt atgatgaaag aagagcgtta tcatacacta ataaatcata
    16981 ttgataatca tttagacaat tctaaaatac gtgaagtaat gacgatagat aacaatgatg
    17041 aacagttctt taagaagaaa tatcaagcac aagaacgaga aatcattttt aaagaagata
    17101 gagaagatgg ttatacatta taaaaataga tatttaaatg catttgtttg aggttaatta
    17161 ttttttagga tgaaaaatag gtagcccccc tcgaactttt taatatattt ttataaatct
    17221 ttttaaaaac ctttttaggg gcttgttcag ccctagagca acaagggatt tcaatgccta
    17281 aaggagtgtt tttgccacat aaaaggagtg tttttgccac ataaaaggag tgtttttgcc
    17341 acataaaagg agtgtttttg ccacataaaa ggagtgtttt aaaaagttgt actcctttta
    17401 tggtttgaca ttgataataa agtagtttat tgtgaataaa agttttcaaa tgggagtgta
    17461 tattcaaacg atactccttt aggttcgtaa taaagggggc tgtatcatga caggagagac
    17521 agttgtatat aaaaatgaaa tgaatttagt tccattaagg cgatttacag ctactgaaat
    17581 taatttattt tttgcgatgt gtaataaatt aaaagagcaa gatactaata cattacgttt
    17641 gtcttttgat gaattaaaaa aattaagtaa ttatagtcca gaaacacgta atattaatag
    17701 atttgctaat gatttagata atgtgtataa aaagatgttg aatttaacca ttagatatga
    17761 agatgatgat gtaatagaaa gattcgtttt attcaatcat tatagaatcc ataagcgaga
    17821 acaatatcta gaaatatcaa cttcttcaaa tttaaaacac atattgaatt caattactaa
    17881 taattttaca aaatttgaat taaaagaaat gactaggctt aaatctacat attctaaaaa
    17941 tatgtttaga ttacttaaac aatacaaaca tacaggctat ttaaaaattc acatcgatga
    18001 ttttaaaaat cgtttagata tacctaaatc ttatcgtatg actgatatta acaaaaatgt
    18061 ttttaaacct attattattg aattaggttc tatatttaat aatcttacta tcaataaaat
    18121 caaagcgaaa aaagggcgta aaattgaatg gatagagttc acatttgacg ctgagaaacg
    18181 cattcataac aaacgacaac ctcaaatgtc caagatagat aaatcaagac aatatgtgag
    18241 gcgtgaaaag acgcctaaat ggttagaaga acggtcatat gaaaagcaac ctcaaaaaga
    18301 ttacgaccca caattagaga aagaacgaga gaatttttta aaacaacttg aactgaattg
    18361 ggaataacgc aacttttcgt tgcgagttgg ttaactaaaa gtatcgtgac ttttcaatta
    18421 ttttaattat agtttgagtt aaggagtgat tttatgaaat tagctgaagc tataaaagaa
    18481 cagagagaat taaaaggatg gtctcaagag gaattagcca atatattaaa ggtttccaga
    18541 caaagtgttt ctaaatggga aagtgccaaa aactatcctt ccttagatat tttgattgct
    18601 atgagcgatt tattcggaat ctctttagag catttaatta aaggagatgc acgttttaaa
    18661 aaagttattt tagaaggaaa ttatagagag agtaacaggg taccttccat agttgatttt
    18721 ctttatgatt tttggtggct tttctttcct gttgccggat ttttagtttg ggccattcat
    18781 tcgttcatgg gttgatttta aaatcaaaat ttaatgcaca tcaggcaata tgaaaaagga
    18841 aagtattttt acgacacctt gaaatgtatt gataagaata gaagagttta caaaaggcaa
    18901 tatgaaatat atactaaaag taacaagaat ttcgtttgta aacgcaccaa accccgaact
    18961 attcgccgta gttcggggtt ttttggtgaa ttggctttaa tcgtctatat tattttttat
    19021 cacgtttatg gagctaataa ccaaataacg taacgagaca accactgatt gccgttgtca
    19081 tgatgtgaac aagaatttca ttcataaatc gcacctcctc tctacgccga attgacatct
    19141 gagagataga cgaccttcac attataacaa atctctcctt ccatgaaata acatgaatat
    19201 taataaatgt tgttatatat taaagataaa taaatataag ggtgggattg ataatgtttg
    19261 gcgatcaaag acaagaagct acaaaatatg tcattaaaga aggttatcaa gatatttatt
    19321 tcctcaacaa aaacggggaa tggtattatt tcgaagtaag aagtgtctgg cgtggcaaac
    19381 atattattag agttaaagat ggtttgttag gttggagaaa agaaatagta actgaataaa
    19441 gatacaaaat acttgccaat gcaaaagaat gaaaacgcgt caaatcaacg atgtataaaa
    19501 gatgcataaa agataatgaa agtaaaaagc cataaatgcc gtcatatagg catttatggc
    19561 tttttgtttg gttaatataa tcgatgatta tcagaagttc aattcacata aaaaagcacg
    19621 gctaatttat gaacttgtcg taccttttta attaaaatta gttaccaaat agacgactcc
    19681 aaaacccctt tttagcttcc gtttcttcac tcttttcttc tatatcacta tcattgtcat
    19741 ttggattagc tgaaacgtct tctctttgtg cttcttcacc ttgtgtactt tcgtcgcttt
    19801 gacttccaga tatatcctta tattgaatct ctgtttcttt agtttcttcc tgatctcggt
    19861 taatatttac aggactagta gtatatgttt tttcttgtgc atcaacgttt tgtctagcgt
    19921 tagtagatgt atcaaaggga taattaaggt gtttttcttt ttctaattga ttttctaatt
    19981 tttgaatctt tttattactc tctaatgcca gaacttgctg attctcaagc aattttgatc
    20041 tttcttgcaa ctcttttctg taaaattcaa ttgttgtttc atgacgactt tcttgttttt
    20101 caatttgatt atttaaagta ttaatctgat ttttaagtat ttctactatt tgagcatcgc
    20161 ttttagattc aaaattaata ttatccgact cttttttttg tttgtcttca gtagcacttt
    20221 caaaaccata tttcttttta ttttcattta ctctttgaat gattttttct atatcggtat
    20281 cttctttaat aaacgaagtg ttttcctgtt ttattgtctc tatattcaat ctcttaatat
    20341 tattaaaaat cgtttgctta ctaaccccta attcttcact caaccttttt acacttttca
    20401 tatcatcaac cctttataaa atgtttataa acccttagta aaatattata agcttataac
    20461 gattaataat aaagtaatcg acaaataaaa atataaattc gccaaccttt ttatcaaggg
    20521 tttgtaaagg gtttatggtg tttatcaaag gtttataaac ctatattttc ccttttttaa
    20581 gtttattcaa agtgctataa taaccttaat ataacaaaaa ccccaactac tggtaatagt
    20641 cggggt
//
