LOCUS       NZ_CP086573            20644 bp    DNA     circular CON 04-JUN-2024
DEFINITION  Staphylococcus argenteus strain RIVM_M036020 plasmid
            pRIVM_M036020_1, complete sequence.
ACCESSION   NZ_CP086573
VERSION     NZ_CP086573.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22560076
            Assembly: GCF_023612155.1
KEYWORDS    RefSeq.
SOURCE      Staphylococcus argenteus
  ORGANISM  Staphylococcus argenteus
            Bacteria; Bacillota; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20644)
  AUTHORS   Witteveen,S., Hendrickx,A.P.A., de Haan,A., Landman,F., van
            Santen-Verheuvel,M.G. and Schouls,L.M.
  TITLE     Genetic characteristics of Staphylococcus argenteus isolates
            collected in the national Dutch MRSA surveillance
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20644)
  AUTHORS   Witteveen,S., Hendrickx,A.P.A., de Haan,A., Landman,F., van
            Santen-Verheuvel,M.G. and Schouls,L.M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Bacterial Surveillance and Response,
            National Institute for Public Health and the Environment, Antoni van
            Leeuwenhoeklaan 9, Bilthoven 3721 MA, Netherlands
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 30x
            Sequencing Technology             :: Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023612155.1-RS_2024_06_04
            Annotation Date                   :: 06/04/2024 06:01:02
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,747
            CDSs (total)                      :: 2,664
            Genes (coding)                    :: 2,591
            CDSs (with protein)               :: 2,591
            Genes (RNA)                       :: 83
            rRNAs                             :: 7, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 7, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 38 of 73
            Pseudo Genes (incomplete)         :: 34 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 16 of 73
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086573.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..20644
                     /organism="Staphylococcus argenteus"
                     /mol_type="genomic DNA"
                     /strain="RIVM_M036020"
                     /host="Homo sapiens"
                     /db_xref="taxon:985002"
                     /plasmid="pRIVM_M036020_1"
                     /geo_loc_name="Netherlands"
                     /collection_date="2016"
     gene            166..842
                     /locus_tag="LK336_RS13610"
                     /old_locus_tag="LK336_13600"
                     /pseudo=""
     CDS             166..842
                     /locus_tag="LK336_RS13610"
                     /old_locus_tag="LK336_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /name="replication initiator protein A"
     gene            <853..1135
                     /locus_tag="LK336_RS13615"
                     /old_locus_tag="LK336_13605"
                     /pseudo=""
     CDS             <853..1135
                     /locus_tag="LK336_RS13615"
                     /old_locus_tag="LK336_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199895.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006260 - DNA replication [Evidence IEA]"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            2344..2961
                     /gene="cadD"
                     /locus_tag="LK336_RS13620"
                     /old_locus_tag="LK336_13610"
     ARG             2345..2958
                     /name="cadD"
                     /gene="cadD"
                     /gene_name="cadmium resistance transporter CadD"
                     /tool="amrfinderplus"
                     /drug_class="CADMIUM"
                     /identity="99.51"
                     /coverage="100.0"
     gene            2980..3327
                     /locus_tag="LK336_RS13625"
                     /old_locus_tag="LK336_13615"
     CDS             2980..3327
                     /locus_tag="LK336_RS13625"
                     /old_locus_tag="LK336_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020366422.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="WP_000119691.1"
                     /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKKL
                     KIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDDEI
                     REFFSKNQEGF"
                     /name="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            3487..3579
                     /locus_tag="LK336_RS13630"
                     /old_locus_tag="LK336_13620"
     CDS             3487..3579
                     /locus_tag="LK336_RS13630"
                     /old_locus_tag="LK336_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_001791613.1"
                     /translation="MLIIFVHIIAPVISGCAVAYYTYWLSKRNK"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            3791..4765
                     /locus_tag="LK336_RS13635"
                     /old_locus_tag="LK336_13625"
     CDS             3791..4765
                     /locus_tag="LK336_RS13635"
                     /old_locus_tag="LK336_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="WP_000072390.1"
                     /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFNS
                     SSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFEMN
                     KASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLMQSVPSEIKENSGWI
                     RVQDCGKNVKKLKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIEDIEN
                     YLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYEEDLKL
                     LQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE"
                     /name="DUF4062 domain-containing protein"
     gene            complement(4885..5700)
                     /locus_tag="LK336_RS13640"
                     /old_locus_tag="LK336_13630"
     CDS             complement(4885..5700)
                     /locus_tag="LK336_RS13640"
                     /old_locus_tag="LK336_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467708.1"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000164958.1"
                     /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSSR
                     YYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAIIDL
                     GQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMIFIG
                     MPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWQDLGFDLKLEDFTDYEAIT
                     TIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR"
                     /name="AAA family ATPase"
     gene            complement(5693..7136)
                     /locus_tag="LK336_RS13645"
                     /old_locus_tag="LK336_13635"
                     /pseudo=""
     CDS             complement(5693..7136)
                     /locus_tag="LK336_RS13645"
                     /old_locus_tag="LK336_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790716.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /name="Mu transposase C-terminal domain-containing protein"
     gene            complement(7108..7701)
                     /locus_tag="LK336_RS13650"
                     /old_locus_tag="LK336_13640"
     CDS             complement(7108..7701)
                     /locus_tag="LK336_RS13650"
                     /old_locus_tag="LK336_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690636.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000690638.1"
                     /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPGL
                     DKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSSTGQ
                     LLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNGTPI
                     KTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN"
                     /name="recombinase family protein"
     gene            complement(7965..8345)
                     /gene="blaI"
                     /locus_tag="LK336_RS13655"
                     /old_locus_tag="LK336_13645"
     ARG             complement(7969..8345)
                     /name="blaI"
                     /gene="blaI"
                     /gene_name="penicillinase repressor BlaI"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="99.21"
                     /coverage="100.0"
     gene            complement(8335..10092)
                     /gene="blaR1"
                     /locus_tag="LK336_RS13660"
                     /old_locus_tag="LK336_13650"
     ARG             complement(8339..10092)
                     /name="blaR1"
                     /gene="blaR1"
                     /gene_name="beta-lactam sensor/signal transducer BlaR1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="95.38"
                     /coverage="100.0"
     gene            10199..11044
                     /gene="blaZ"
                     /locus_tag="LK336_RS13665"
                     /old_locus_tag="LK336_13655"
     ARG             10200..11041
                     /name="blaZ"
                     /gene="blaZ"
                     /gene_name="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            11413..11946
                     /locus_tag="LK336_RS13670"
                     /old_locus_tag="LK336_13660"
     CDS             11413..11946
                     /locus_tag="LK336_RS13670"
                     /old_locus_tag="LK336_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823239.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001823239.1"
                     /translation="MIVGYARVSSNDQNLERQLENLKTFCAEKIFTEKQSGKSIENRPV
                     LQEALNFLRMGDRFVVESIDCLGRNYDEVVGNPLLDKFMKDLIIQILAMVSEQERNESK
                     RRQAQGIQVAKEKGVYKGRPLLYSPNAKDPQKRIIYHRVVEMLEEGQSISKIAKEVNIT
                     RQTIYRIKNDNDLI"
                     /name="recombinase family protein"
     gene            12360..12653
                     /locus_tag="LK336_RS13675"
                     /old_locus_tag="LK336_13665"
     CDS             12360..12653
                     /locus_tag="LK336_RS13675"
                     /old_locus_tag="LK336_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000726503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="WP_000726502.1"
                     /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGKH
                     YVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH"
                     /name="lactococcin 972 family bacteriocin"
     gene            12728..14662
                     /locus_tag="LK336_RS13680"
                     /old_locus_tag="LK336_13670"
     CDS             12728..14662
                     /locus_tag="LK336_RS13680"
                     /old_locus_tag="LK336_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="WP_000713729.1"
                     /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNWD
                     KSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLTPS
                     DLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLVPFI
                     FLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFIANIL
                     IIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKGYKFSI
                     FMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNIRNSEEK
                     QILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNFYKNINHD
                     FKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSKNVSVFSFD
                     RTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLKDNHLEEEIS
                     GITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWNKKQLLLRKIH
                     GYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHLLQIAYIKYLEK
                     YFKDLMREI"
                     /name="bacteriocin-associated integral membrane family
                     protein"
     gene            14666..14977
                     /locus_tag="LK336_RS13685"
                     /old_locus_tag="LK336_13675"
     CDS             14666..14977
                     /locus_tag="LK336_RS13685"
                     /old_locus_tag="LK336_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="WP_000831610.1"
                     /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVPK
                     DTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKDIPSFLK"
                     /name="YxeA family protein"
     gene            14989..15615
                     /locus_tag="LK336_RS13690"
                     /old_locus_tag="LK336_13680"
     CDS             14989..15615
                     /locus_tag="LK336_RS13690"
                     /old_locus_tag="LK336_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_000593103.1"
                     /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLLN
                     IIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFKKN
                     KKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTGSLD
                     TKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS"
                     /name="ABC transporter ATP-binding protein"
     gene            15853..16482
                     /gene="mobV"
                     /locus_tag="LK336_RS13695"
                     /old_locus_tag="LK336_13685"
                     /pseudo=""
     CDS             15853..16482
                     /gene="mobV"
                     /locus_tag="LK336_RS13695"
                     /old_locus_tag="LK336_13685"
                     /inference="COORDINATES: protein motif:HMM:NF013258.4"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /name="mobV"
     gene            16576..17121
                     /locus_tag="LK336_RS13700"
                     /old_locus_tag="LK336_13690"
     CDS             16576..17121
                     /locus_tag="LK336_RS13700"
                     /old_locus_tag="LK336_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001805533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_031788368.1"
                     /translation="MYEIKTKNVGGWFHKEKQETGNIVITKTDFEKYTKQIKAAQMILD
                     DYECIKSGKSLKKAEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSDNEKKDKE
                     LNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQ
                     AQEREIIFKEDREDGYTL"
                     /name="hypothetical protein"
     gene            17505..18365
                     /locus_tag="LK336_RS13705"
                     /old_locus_tag="LK336_13695"
     CDS             17505..18365
                     /locus_tag="LK336_RS13705"
                     /old_locus_tag="LK336_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145511.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_031788340.1"
                     /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLRL
                     SFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIHKR
                     EQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLKIHI
                     DDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEFTFDA
                     EKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERENFLKQL
                     ELNWE"
                     /name="RepB family plasmid replication initiator protein"
     gene            18452..18793
                     /locus_tag="LK336_RS13710"
                     /old_locus_tag="LK336_13700"
     CDS             18452..18793
                     /locus_tag="LK336_RS13710"
                     /old_locus_tag="LK336_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761340.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000761341.1"
                     /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSLD
                     ILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAGFL
                     VWAIHSFMG"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(19032..19103)
                     /locus_tag="LK336_RS13715"
                     /old_locus_tag="LK336_13705"
     CDS             complement(19032..19103)
                     /locus_tag="LK336_RS13715"
                     /old_locus_tag="LK336_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002495983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_106873653.1"
                     /translation="MNEILVHIMTTAISGCLVTLFGY"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            19252..19437
                     /locus_tag="LK336_RS13720"
                     /old_locus_tag="LK336_13710"
     CDS             19252..19437
                     /locus_tag="LK336_RS13720"
                     /old_locus_tag="LK336_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000466619.1"
                     /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHII
                     RVKDGLLGWRKEIVTE"
                     /name="hypothetical protein"
     gene            complement(19656..20399)
                     /locus_tag="LK336_RS13725"
                     /old_locus_tag="LK336_13715"
     CDS             complement(19656..20399)
                     /locus_tag="LK336_RS13725"
                     /old_locus_tag="LK336_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_000843757.1"
                     /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDIE
                     KIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEKQE
                     SRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTSTNAR
                     QNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSANPND
                     NDSDIEEKSEETEAKKGFWSRLFGN"
                     /name="DUF536 domain-containing protein"
     ARG             10200..11044
                     /name="PC1 beta-lactamase (blaZ)"
                     /gene="PC1 beta-lactamase (blaZ)"
                     /gene_name="BlaZ beta-lactamase"
                     /tool="rgi"
                     /drug_class="penam"
                     /identity="96.8"
                     /coverage="100.0"
     BGC             7360..17653
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          1193..1340
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="81.333"
                     /coverage="100.0"
                     /evalue="2.96e-25"
     Typing          17506..18365
                     /name="rep_cluster_2214"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.884"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(19657..20399)
                     /name="rep_cluster_1733"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          15854..16128
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.565"
                     /coverage="100.0"
                     /evalue="4.71e-46"
ORIGIN
        1 ttggtaataa aacgtttaga aactgtttat ttatataatt ttcgatactt agattatata
       61 atgaataata tcttagtgca agcacctaga ttattaaata aacacaaaaa atgtaagcgt
      121 tttattacct cttaccaaac taaaattcgg aaagaggttt ttattatgtc aattaaactt
      181 tacaacataa atgaggaata cagagaaaag ttttatcaaa taccgaaagt attttttaca
      241 aatcctaaat atatcaagtt atccaatgat gcaaaaatgg catggggtat tcttagagat
      301 cgtttagatt tatcaattaa aaatggttgg gttgattcta aaacaggtaa tatttatttt
      361 tattacaaaa atgaaaaccc acaaaatata ttaaattgta aaaaagataa agtaattaaa
      421 ataaagaaag aacttaattg aagccgaatt actactacaa gaaagacaag gtttaaataa
      481 gacaaataaa ctttatttag ttaaaccaga agtaacagaa gatgatattt ataaaattca
      541 aaagcaagaa aacactgttg agagcagcaa tggcaaggaa gtcggaaaaa ccgacttcaa
      601 gaagtcgata aaacagaatc taatgatact gaacttagct atactgaatc taacaatact
      661 gaaataaatg atatgaatga cttgaatgat atagagtccg aaaatgaaat tagtaatcat
      721 tcaagtcaat gcaaggttat ttaatgcaaa caattttaac tgaatttgaa gaaacacatg
      781 cactttatat gcgccgtaaa aacatgaaac aatataatat ctttaatcaa tcattcaaat
      841 gattttataa caatcaaaga tacatcaaaa tatttcgtta aatcatctga ctttttaact
      901 gatagcgatg aaaaaatcaa gaaattgtca gtgatctaga aaaagatttg tatcgaaaac
      961 gtatgttaag ttatggtgga ttgcttaaat aaaaacataa aattttaaac ttagacgatg
     1021 ctgaagatgg caatttaatt aatacaagtg atgaagataa aatagcaaat gaagaagaaa
     1081 aggcacattc gattacggca attttgaaaa gcaaaattat tatttaaggc attagtgata
     1141 actgatgtct ttttttatta cttttttgta aaaaaagtgc tgtcttattt ttgtgacaaa
     1201 tgctgtatgt agtgtcacaa aagtgtgaca cttgagcttt ttatgacccc acacaaaaac
     1261 attttcgaag ccttgaaata tctggctttg ccagatcccc cattcattga tggggacaaa
     1321 tttcccttat gctcttacgg agattttaga gttaaattaa aatttctaag tttttgataa
     1381 gaaaagccct gcaggaattt agaagaacgt ggggaagttt taaagaattt tatgctaatt
     1441 tttaatttgc atgtaaatgg gcagtgtcta aaaaatagtt attgtttttg ctcaaatttt
     1501 tgtttttgaa aatagcatat atttatttgg ctcatatttg cgttttaaaa gcgtatataa
     1561 gttttagata aaactatatg aatcacccct aaatctttaa aatgccctct taaaattcaa
     1621 aataagggca taaaaaataa aagccccccc agataaagtg aggtagcttt ttctttcgac
     1681 gccgtatcgt cgatgtaatt caaaaaagtt atgggctata aatctacatc actttttcaa
     1741 gaatgtagta gcaatattca acgcaaatta atttttataa ctgctcggaa tatctcaagc
     1801 cgtttcttta attttgaaat aaaaaaatcg acgaaggtcg attacgtttt tgcacatgtc
     1861 gatttatccg acgtataagt gcgcccttac gggatttaac tagattataa cgacgaattt
     1921 tagacctgta aagcaataac aattaggtct accaggtaga taaagtgtac aaagtagaac
     1981 cgaaaaagtt atttattttg aagatttttc gtagatataa tttgctaata aacttcctaa
     2041 aactgaataa agaaaaatag ttaaactcat gtaatcactc cataattctg aagtgtctcc
     2101 atgtctacgc atagacacgc atttatgccg agaaaattta ttgtgcgttg agaagaaccc
     2161 ttaactaaac ttgcagacga atgtcggcat agcgtgagct attaagccga ccattcgaca
     2221 agttttggga ttgttaaggg ttccgaggct caacgtcaat aaagcaattg gaataaagca
     2281 cctatgatta tttaaaaata taagttataa tacattcaaa aatatttttg aatgaggtgt
     2341 attatgattc aaacggttgt tgctgctgct gttctttata ttgctacagc agttgattta
     2401 ttagtaattt tattaatatt ttttgctaga gcaaagacta gaaaagaata tagaaatatt
     2461 tatgttggtc aatatttagg gtctattatt ttaatattag ttagtttgtt tttagctttt
     2521 gtattaaatt atgttccaga aaagtggata ttaggtttat tgggtttaat accaatttat
     2581 cttggaatta aagtggctat ttatgatgat tgtgaaggag aaaagagagc taaaaaagaa
     2641 ttgaatgaaa aaggattgtc taaattagtt ggtacggttg caattgttac gatagcaagt
     2701 tgtggcgccg ataatattgg tttatttgtt ccttattttg tgacattaag tgttactaat
     2761 ttattactta ctctgtttgt ctttttaatt ttaattttct tcttggtgtt tactgcacaa
     2821 aaattagcta atatcccagg aattggagaa attgttgaga aatttagtcg ttggattatg
     2881 gctatcattt atatagcttt aggtttattt attattattg aaaatgacac tattcaaaca
     2941 attttaggat ttatatttta atttagggtg tgattttata tgagttatga aaatacttgt
     3001 gatgtgatct gtgtacatga ggataaagtt aacaatgctt taagtttttt agaagatgat
     3061 aaatctaaga aattacttaa cattttagaa aaaatttgtg atgagaagaa attgaaaatc
     3121 atattatctt tgattaaaga agatgagttg tgtgtttgtg atatttcttt gatattgaaa
     3181 atgagtgttg cttcaacttc acatcattta aggcttttat ataaaaatga ggtacttgat
     3241 ttttataaag atggaaagat ggcatattat tttattaaag acgatgaaat aagagagttt
     3301 ttctctaaaa atcaggaggg tttttgaaac gagtttcttc ttgtcttgat actataaaaa
     3361 acaaagttat ataacataag acgacttatc ggaagttatc tatcatcgta tattcggaga
     3421 tgatataata tacaggtcgc ctatctctca ggcgtcaatt cagacgcaga gaggaggtgc
     3481 attactttgc taatcatttt cgttcacatc atagcaccag tcatcagtgg ctgtgctgtt
     3541 gcgtattata cttattggct tagtaaacgc aacaaataat taataggcga ctttataagc
     3601 cagcatcgaa aaaatcccca cactattgca gtagtgtggg gattggtgca ttaattgtta
     3661 atcattttcg ttatctagat tataacactg gttagtagag gaatgcaaaa agataatatt
     3721 ttaagtagtc tttttggata ttatcatatg atatatttat gtaaattgat tttttattaa
     3781 aggagcatat atgagtaata ctaaaattaa atatcaaatt tttatttcat caacttattt
     3841 agatttaata gaagagcgtc aagcagtagt agaagcagtt ttaaacagta ataatattcc
     3901 agcaggtatg gaacttttta actcaagtag taaaagccaa tgggaaataa taacacaatg
     3961 gatcaatgaa tcagatgcag tgatattatt gttaggttct agttatggaa caattgatag
     4021 tgaagaaaat attagctata cagaaatgga gtacaatttt gctaaatctt taggtaaacc
     4081 tatatatgtt tttgagatga ataaagcatc taccgaatcc gcggaaaagt ctaaaaaatt
     4141 agatgagttt aagacaaaag ttaaagaaaa tagactaatt agtccaaata ttaatgatat
     4201 ttcggaatta aaaagtaaag tatctcagac tttaatgcaa agtgtaccta gtgaaattaa
     4261 agaaaatagt ggatggataa gagtacagga ttgtggtaaa aatgtaaaaa aattaaaaaa
     4321 attggatgct atacaaagag atttagatac atatgatttt ttgaaagaag aactttataa
     4381 aatattcatg tatgtaaaaa ctttttattt ccagcacaca ccatttgatg atagttatat
     4441 tgaagatata gaaaactacc tgctaaatta tgaaaataat ccacataaat cattcatgaa
     4501 aataaaagtt tatgattata aaaacttttt aaaacattta aaaaacatat tgattttaat
     4561 taaacaagga aaaaagccat tagctcaagc cggtcatttt aaattaagct tttacgaaga
     4621 ggatttaaaa ttgttacaaa aaaattataa ggctacgcta gtcgatatta aaggattgaa
     4681 taaacatttt tataaggaat gtgataggtg cttaaaagca tataatattt ttattgaggg
     4741 tataaataaa catttatatg aatgatataa gtacacccta atcatacata tataattgtt
     4801 gtcactttac tcgatatatg agaatgactt tacctaataa atgagaaaag caacgcacta
     4861 tattaaaatg cgttgctttt tctattatcg aatacctata actaaactat ctctagctgt
     4921 ttctacaact tctgtactta ttttatctaa accatttata tccataattc tgtctatctg
     4981 cgcaaataaa cgatgaatca gcctaaaatt cccttttgta attttaataa tcgtcgttat
     5041 tgcctcataa tcagtaaagt cttcgagttt taaatcaaat cctaagtctt gccacttata
     5101 ttctaatata tgatgcgtct catctttact tagattatca aattcatgcg caaaacctat
     5161 acgcgaatat agttgaggat aacgagatag ttttttctct atacccggca taccaataaa
     5221 tatcattgct aaattatgtt catcataaat agctcttaat tgctctaaat gttgcacttt
     5281 aaggcgatca atctcatcta ctataattaa atcaatatat ttaaacatat cactataatg
     5341 tttactatgt tcatgtccat acttatttac aacatgtaat ttttgaccca aatcaatgat
     5401 tgcagtaata ctatataaat cattccttaa tttagtttgt ttttctgctg gtgcattata
     5461 aaatatttta tccaccgata gtatgtcatc ggtcgtttta ctagctaaat cttcccaacc
     5521 tctatggtta acttgttttt caatagtatc ccaatttgtg taatacctag acgataaagt
     5581 ttttccgaca ccaggttgac cataacatat accaatatac tgatatttaa tacaagcatc
     5641 acaaaattca gcaaagcgtt tatactcttt cgtttcaatg aaattttgat ttttagtcat
     5701 tacgatatct ctttaatttt ttgacgtttt tagtcgtttc ttgaggtgaa taaccacaat
     5761 ttttttcttc cttaattaat tccgtggtac ttggactagc aatattttgt tttaagtgtt
     5821 ttcttctctg actacgagcg tgctgtatgt cttttatatc tatggagtaa tcagctattt
     5881 ctggacatat agcagtacaa agaaattcat ctctataaaa cacacgtatt tcagccatat
     5941 catttgggtt ataacgaatc agtacatatt cacctacata agcttctaga ttagtattac
     6001 tgtatcgaaa accttgaaaa tgaatgccat ctgaatgaac ttttctagat ttaggtattt
     6061 ccaacaataa taaatcaagt tgttctaagc tacttggcat attagggaaa aagtgattac
     6121 tattccaatg actgataggc gtggtatgaa tagtactatg ttctttttga ttataatctt
     6181 caattaaaaa gtaacgtaat ttttcttcaa aattgtgaaa atttattaga ttactaggag
     6241 tatcattatt gtttatatat ccaggcagtt gttctaagaa agtttgatta actgtctgaa
     6301 agaatcgttc tatttttcca cgaccacgag gaacaccaac tttcgaaaac atcaagttaa
     6361 ttttcaaatc aatagcgact tgttccatat gatgtgaagt aaagtcactt ccatgatctg
     6421 tgtagaattt ttcgggtata ccacacactg gccaattggt atcattttta ttccatattg
     6481 cttggtgtag tgttaatgct gtgttttgag cattgggggg catcaaaact taagaaatag
     6541 cctgcaattg cacgactata gtcatccata ataatagtta gccatggtct attgatatta
     6601 cctttttggt ctaatataaa gatatctagc aaagtatggt ctgcttgcca tatttcattg
     6661 ggtctcgagg attctctaat ttgtattaaa tcgtacttat tttgatagta tttttcaccc
     6721 tgatgagaga aatcaataac agacttaggc atcgctttaa taacactata aatttgttta
     6781 taacttggta tatcaaaatt attttcttta cagtaattag tgattttcct atgaattgtg
     6841 gctatactat ttcttttatt cataagcgca aggcgttcaa tttcatcaat tatctttggt
     6901 tctattttac gtgttccttt atcagctctt gtcgcataga ttagtccttt aagtccttgt
     6961 tctttatatg acttattcca cctataaagg gtgcttaacg caatcccttt acttttagaa
     7021 atacttgata aagattgttc gcctaggata aaaggtttta taatattgta cttttgagta
     7081 gcttttttac gtttggcttc agaaaaatta gttagatact tctccttgtt tttcatcttc
     7141 cttgtcctcc aatttattga ggtaacgata aattgttgta cgtgaaacat gccactgttc
     7201 tgcaattgtc ttaattggcg tgccattatc ataaagggtc ttaagtaagt ttaaatcttt
     7261 ttgatttaat ttttctggtc tgcctccata tcggcctcta gcacgtgctg caattcggcc
     7321 agctgaagaa cgctctaaaa ttaaattacg ttcaaattct gcaaatgctg cgaataaatg
     7381 aaataataat tgtccagttg aacttgattt atccattgtt atattttctt ctaaactatg
     7441 gaaacttaca cctcgttcgt taagctcatt aactaatgtg attaaatcct ccatattacg
     7501 tcccaatcta tctaatctcc aaacaacaat cgtatctcct gacctcgcaa attcaattgc
     7561 tttatctaaa ccaggtcttt tactttttga accactcata tggtcattaa atatcttttc
     7621 acaaccatat gtatttaatc gatcctcctg taaatttaaa ttttgtaatc cagttgaaac
     7681 tcttgcataa cctattttca aaatacaaac ctcctatata catcttcagt gtaccataac
     7741 ttaattacaa ggtcttttat gaacatagat tttttgttgg gtttttgaac acataatcct
     7801 atgtaattct tacttattta gacataaaag aagagtgtac agaaaatggt tattttctgt
     7861 acactctcat ctttaataat atattttaaa tgtcattaac aaaactaatt taataagagt
     7921 caagcatagt ttacatatgt attatgtttg aaacatacct ctaattactt tttactaata
     7981 tcatttaaaa tatctcgcaa ttcttcaatt tctttgttat ttaattcttc atttttcgca
     8041 aaattcaaca ctaaactttt catatcccct ccatacagtt tattaagaaa ggttttagca
     8101 gttttcattt taatatcgtc ttctttaata tttgatgagt aaaaataaat attttctgat
     8161 ttgtatcgtt ttataatctc ttttttatat agtcttgtga ttaatgttct aatcgtttta
     8221 tcactaactt ctttatattt ttgaatttca actacaattt cattagctga tactgatttt
     8281 ttatcccata ttatattcat aacatcccat tcagccatag atatttcaac ttgcttattg
     8341 gccatttaaa acacccattc cttttaatat tttttcactt attaattcag catttttccc
     8401 agacgggttt ccatctgata aatgtgtagc aaaataatac ttatcatgat ttgtaattac
     8461 gtaacctaca aaccacccat tattatactt cccgtttact atacctgtac ctgttttccc
     8521 atacagttca tacttttcat ttttcttaat caataatgaa gaagataatt gattctttgc
     8581 ttttttacta aaatggttat tttgttccat catattttta aaaactatta cttgttcaag
     8641 attagatatt ttcaaactat cttccatcca atagctttta taacttccca aattttcatt
     8701 accataattt aattgcttga gttgagccgc agtatagttc tttggtattt gattactaat
     8761 acgttcgaag taccaattaa ctgaattttg cattgctgta tttaaatctt gttccttatt
     8821 ccaagactcg aaaagataat gtttatgatt ccatgacata cgtgaatttt tatcacttat
     8881 aatatgacgg tcaagtccaa acagtgctaa ataaattttg taagttgaat caggagaata
     8941 tctttttcta ctttcttttt cattataaat atagtatttg tctttcttca tgctgtacat
     9001 aacgaaagat ccactatttg aaccaaaatt tttactttca tctaaaattt ggtaatcgct
     9061 ttgtaaggga tttttataat tataatcagt taaggattgc cccattaaaa actggctttg
     9121 aattatcatg ataaaaaaag taaaaataca aataaaaatt agaattaatt ttgactgctt
     9181 tttcagatcg gcttctttta tattaattaa tctatttttg agtaatgatt ttttaccatt
     9241 aaatgaatgg cttaatatat ttttgttgaa aaatgaggtt tttaatacgg agtccataat
     9301 agattccgca taagttttaa attcattttt gttaatatta ttaaggacaa atctatcggc
     9361 ttctacttca ttgtcatgaa ttatctttct ttttacaata tgtactaatg gattgtaact
     9421 cataataata gagaagatat taaagataat taaatgtaaa gtatctctat ttttagcatg
     9481 agcatattca tgcagtatta tatattttag tcttttatca attacacttt taaaatatga
     9541 actaggaatc aaaataatat atttcccata ccaaaaagtt attggagatt gaatagcctc
     9601 tgcttttcga atcacaatat tttttttata ttgatggttg aaaagtatcg tatctatttt
     9661 atttttttca ttttcgttta gataaagtga ctgtttcttt aaatatttaa gatataataa
     9721 ggctttcaaa aatttaaaac ttaaaataat aactaaaact atccaaataa ctgtgcagat
     9781 attatcaatt gaatcccaat taaacttatg gatatctgtt gcgaactctt gaacaggttt
     9841 ggtggtaatt atgttatggt tcaagtcgtg tgacttactt tcaactgtgg gcgcttgatt
     9901 attcacatta ttaaatttta taaaagagaa tttaattggg ataaaaggaa ttaatccagc
     9961 aagcacagtt agataccaaa ctttataatt taaactataa ttaaaatagc gttttaatat
    10021 gtacctaaaa aatactaaca atagaaatat aaaacaaaag cttactacac tcattattag
    10081 taatttagcc attttgacac cttctttcaa atatttataa taaacaattg acatcaatat
    10141 tacaattgta atattattgt tttgtaaaaa ttacaactgt aatatcggag ggtttatttt
    10201 gaaaaagtta atatttttaa ttgtaatggc tttagtttta agtgcatgta attcaaacag
    10261 ttcacatgcc aaagagttaa atgatttaga aaaaaaatat aatgctcata ttggtgttta
    10321 tgctttagat actaaaagtg gtaaggaagt aaaatttaat tcagataaga gatttgccta
    10381 tgcttcgact tcaaaagcga taaatagtgc tattttgtta gaacaagtac cttataataa
    10441 gttaaataaa aaagtacata ttaacaaaga tgatatagtt gcttattctc ctattttaga
    10501 aaaatatgta ggaaaagaca tcactttaaa agaacttatt gaggcttcaa tgacatatag
    10561 tgataataca gcaaacaata aaattataaa agaaatcggg ggaatcaaaa aagttaaaca
    10621 acgtctaaaa gaactaggag ataaagtaac aaatccagtt agatatgaga tagaattaaa
    10681 ttactattcg ccaaagagca aaaaagatac ttcaacgcct gctgctttcg gcaagacttt
    10741 aaataaactt atcgcaaatg gaaaattaag caaaaaaaat aaaaatttct tacttgattt
    10801 aatgtttaat aataaaaacg gagacacttt aattaaagat ggtgttccaa aagactataa
    10861 ggttgctgat aaaagtggtc aagcaataac atatgcttct agaaatgatg ttgcttttgt
    10921 ttatcctaag ggccaatctg aacctattgt tttagtcatt tttacgaata aagacaataa
    10981 aagtgataag ccaaatgata agttgataag tgaaaccgcc aagagtgtaa tgaaggaatt
    11041 ttaatattct aaatgcataa taaatactga taacatctta tagtttgtat tatattttct
    11101 attatcgttg acatgtataa ttttgatatc aaaacctgat tttcccttta ttattttcga
    11161 gatttatttt cttaattctc tttaacaaac tagaaatatt gtatatacaa aaaatcataa
    11221 ataatagatg aatagtttaa ttataggtgt tcatcaatcg aaaaagcaac gtatcttatt
    11281 taaagtgcgt tgcttttttc tcatttataa ggttaaataa ttctcatata tcaagcaaag
    11341 tgacataatt gttgaattta tagtaactga aacgtatgat tagggtgtat attaattatg
    11401 agatggaaat caatgattgt tggctatgcg agagtatcat cgaacgatca aaatttagaa
    11461 agacaacttg aaaatttaaa gacgttttgt gctgaaaaaa tatttacaga aaaacagtct
    11521 gggaaatcta ttgaaaatag accagtactt caagaagcac ttaacttttt gaggatggga
    11581 gatcggtttg tagtagaatc cattgattgt ttaggtcgta attatgatga agtggttggc
    11641 aatccattac tggataagtt tatgaaagat ttaattattc aaatattagc aatggtttca
    11701 gaacaagaga gaaatgaaag taaacgtcga caagcgcaag gaattcaagt tgcgaaggaa
    11761 aaaggcgtat ataaagggcg ccccttactt tattcaccaa atgctaaaga tccacagaaa
    11821 cgtattattt atcatcgcgt tgtagaaatg ttagaagaag gccaatcaat tagtaaaatt
    11881 gcgaaagaag taaatataac aaggcagacg atttacagaa ttaaaaacga taatgatttg
    11941 atttaataaa ctttgtttca atgcaactga gagcttctca cagcgtctca aaacagatga
    12001 ttataatcta ttatgagttt ttaataataa gacaagaaaa gagagcgtaa atatgctctc
    12061 tctttttctc attaacgaac gtttataacg caatcatgat gagagtatta atacagttaa
    12121 ttatttgaaa tactttagtt tatataatgt tgaattatag gtagtgaaat tgtatttatt
    12181 atacattgtc atatcgttgt ttaatgatag ttaatttaag gataatagaa ttaatattat
    12241 atatagatga acaaaacaag caacagagca gaaaaaacac ttttagatta tttttactca
    12301 ttctctattt tatttatttt tgctataata aataaagaat attaaatgga tgtgttttta
    12361 tgaaaaagaa atttgtttct agttgtattg ctagtacaat cttattcggc actttattag
    12421 gtgtaactta taaagctgaa gctgcaacag tacatgtagc tggtggtgtt tggagccatg
    12481 gtattggaaa acattacgta tggtcttatt atagtcacaa taaaagaaat catggttcaa
    12541 cagcagtagg gaaatattca tcttttagtg gtgttgctcg acctggtgtt caatctaagg
    12601 catctgctcc aaaggcttgg ggtggaaaca aaacatttta tagtcttcac taattacata
    12661 tattaattaa ggagaataga cagaattaag taaacttaat tctgtctatt ctcttgaaag
    12721 aggcaatatg aaaaaattca aattattttt agattttagt actttattat taatttcagg
    12781 gctattattt ttatttttct tcaaagaaaa tgaagaaatt atacctgaaa gttccaatat
    12841 tttaacaatc tctaattggg ataaatctaa cagtaaatca aaagtgttag atgtcattga
    12901 atcaggcgcc aaaaatcaaa atatacaaat aattaaatcc gttaaagatt ttgataataa
    12961 aaaagaattt tttgttttca actcaaaacg aaataattct gactttatac gtaataaaac
    13021 atcactttta actccatcag atttattaaa cagagagata aaaggaaagt attatattat
    13081 tggtgaacat ttcaatgtag aagagcttac gagtgaatta aaaaaagctg gtttaacatc
    13141 tgaagttgaa tacataaata gaaacatatt attttttgaa ttagtcatag ataatgattt
    13201 attagtgcct ttcatattct taggcgtatt atatcttttg tacttcttat atgacagagg
    13261 atataatttg aaattttatg ctattcaaaa gctacatggc ttttcaacta cacgcattat
    13321 ttttcataat attcatataa aaatcattta ttggctagta cttaccgcta ttttctttat
    13381 tgctaatata ttaatcattc atatagccaa tttagaattg gatttcttaa tgtttattcg
    13441 tagatttatc gtgttgcttt ttgtttttgc ttgtattact acattgctgt ggctgatttc
    13501 gtatagtctt cttttgaagt taagtattcc attaatctta aaagggaaaa aaggatataa
    13561 gttttcaata tttatgggta ccttcaccaa atgtatcatg gtgctaatac tatctttttt
    13621 actggcacaa aatttaaatg cttatacgaa gttaaagaat atatatgact ctgaaaaaat
    13681 ctggcaaaag ttagataatt actatacatt agagatttct cctaatatta gaaattcaga
    13741 agaaaaacaa attttagaaa ctaatatata caatttaatt aagaaaagcg aacaacttgg
    13801 tggattgctt ttaaagaata ataatatagc caatcccgat aaaaacaatt acattcctga
    13861 aaatggcaat gtctttttta tcaataacaa ttttctgaat ttctataaaa atataaatca
    13921 tgattttaaa atgattgaca atcattcgga taaaataaac gtgtttattc ctccaaggtt
    13981 acaatatcaa aaatctgaaa ttcaaaaaaa tcaccaagga tggattgact ttcaaaagca
    14041 acaaaacaaa aaagtagatg tgcaagtact gtcaaaaaac gtatctgttt tttcttttga
    14101 tcgcacaact aatttgaaat ttgggtatct agattctcca atagtaatgt tgttgacact
    14161 tgacgattta acaggagatt tttatttagc tgctgtttct caaggtggat atctatttaa
    14221 agatttagat acacttaaac agttgcttaa agataatcac ctagaagaag aaattagcgg
    14281 tataactaat tacaaggatt ctgtgttaaa tgaattaaat gaaacgaaaa caaaaatgat
    14341 aataacaata gttactatta ttattaacgt tttcattctt ttgatagcta ctgtatttga
    14401 aacaattcaa tattttagct ggaataaaaa gcaactatta ttaagaaaaa ttcatggtta
    14461 ttcattattc agcagtaatg tacgttattt aacaatttct atcttacttt ctattatgtt
    14521 agcttacact acgcatattt tatttggttc aaagatatta ctattcataa taatgtctat
    14581 tgctattatt caacatttac ttcaaattgc ttatattaaa tatcttgaaa aatactttaa
    14641 agatttaatg agggagattt aaacaatgaa aagaacttta attattgtaa caattgtact
    14701 attaatcatc atacttagtc tgttattggt tagaaatgag catttagatc gttttaaccc
    14761 actgttaaaa gaaaaagtat cttatgccaa agttccaaaa gatacccagg actatagaaa
    14821 tataactgta tactctaaaa atggagaaaa aaagagttat aaattggatt ttttaggtta
    14881 tgatcctaca aaagaatatg tgaaagtaaa tcataaaggg aaatatgtgc gttcgataga
    14941 atacattaca aaagatattc ctagcttttt aaaatgaggt gaccgatagt gataaaactt
    15001 gaaaatgttt ttgtaaaaaa aggtaataaa aacattttag acggatgtaa ctttaatttt
    15061 gaaaaaggaa aatcatatgc tttagttgga gaaagtggcg ctggaaaatc tactttactt
    15121 aacataatcg ctgggttcga agatgtatca caaggaagca tatatattga agataagtta
    15181 ctaaaaaaga aagtcgattt ttatagatat acgttagggt atttgtttca aaattttgct
    15241 ttacttgaaa atcaaacaat cagtcaaaat ttagatttag ccctaaagtt caaaaagaat
    15301 aaaaaagata atatgaactt aaagaaagaa gtattaaaaa aggtcggcct agatttagat
    15361 atcaagagaa ttgttagttc tctaagcggt ggtgaacaac aaagagtagc attggcaaga
    15421 ttaatattaa aagatccgaa aatcatttta gctgatgaac ctactgggtc actagatacg
    15481 aaaaatggaa aaatagttat tgatttatta ttgaagctac ttgatgaaaa taaaactatg
    15541 attgtcgtaa cgcatgattt agagcttgcg aaacgatttg atgtcatagt caatatttct
    15601 gagcttagaa gttaagaaat attgataggt atagccatac atagagaaaa aacaatatga
    15661 aatatgatta gtcattactt ttgagttttt taatttaagg actggatttt ccagtccttt
    15721 tttatgatga caaaaaacaa gcctcgcaga gcacacgttt ttaatgactt atttggtttt
    15781 taataagtct agtatgttaa aacttttctg tgtttaggtg ttataatcat atgcagtgga
    15841 agttgattta ttatgtctta ttcaattatt cgagttgtta aagtgaaatc aaaaacaaat
    15901 acaagaggta ttcaaagaca tattcaaaga gaaaataaaa attatgaaaa tatagatatt
    15961 gatttaagta agtcatattt aaactatgat ttagtcaatg atactaagtt tgattttaat
    16021 aaaaaaattg atgagaaaat cgaaaaaaat tataaaggga aacgcaaaat tagaacagat
    16081 gccattaaac atattgatgg attaattaca tcagataatg tgttttttaa tcaattatct
    16141 gaagaagaga caaaatagtt ttttaagcat gctaaagcgt tcttggaaga agaatacggt
    16201 aaagaaaatc ttttatatgc gacagtacat atggatgaga taacaccaca tatgcattat
    16261 ggcgtagttc ctattactaa agatggacgt ttaagtgcga aagaagttgt aggtaataaa
    16321 aaagcattaa ccgaattcca agatagattc aatacgtata ttaataagca aggctatgat
    16381 ttaaaacgag gaatttctag acaacttaca aaagaaaaac atgatcaagt tagccgttat
    16441 aaacaaaaaa cagaatatca taaacaaatg catatgcgct agaagcaaat agaggattat
    16501 ttaaagcaag aaaatgataa attgatacaa aactaccaaa aatcattaga tacgcttaaa
    16561 aagcttctga gtgtcatgta tgaaattaaa actaaaaatg taggtggatg gtttcataaa
    16621 gagaagcaag aaacaggcaa tattgtaatt actaaaactg attttgaaaa atacactaaa
    16681 caaattaaag cagcacagat gatattagat gattatgaat gtattaaaag tgggaagtca
    16741 ttaaaaaaag cagagaaaca aaatgaaagt ttagttaatg aattgactag tgtccatatg
    16801 gaaaatgaaa agctggttga agaatttaat gatttagcac aacgttataa ttatttgttg
    16861 tcagataatg aaaagaaaga taaagaacta aattatacgc ttaagttatt caatcaagtt
    16921 tttaaaatca ttaaaagtat gatgaaagaa gagcgttatc atacactaat aaatcatatt
    16981 gataatcatt tagacaattc taaaatacgt gaagtaatga cgatagataa caatgatgaa
    17041 cagttcttta agaagaaata tcaagcacaa gaacgagaaa tcatttttaa agaagataga
    17101 gaagatggtt atacattata aaaatagata tttaaatgca tttgtttgag gttaattatt
    17161 ttttaggatg aaaaataggt agcccccctc gaacttttta atatattttt ataaatcttt
    17221 ttaaaaacct ttttaggggc ttgttcagcc ctagagcaac aagggatttc aatgcctaaa
    17281 ggagtgtttt tgccacataa aaggagtgtt tttgccacat aaaaggagtg tttttgccac
    17341 ataaaaggag tgtttttgcc acataaaagg agtgttttaa aaagttgtac tccttttatg
    17401 gtttgacatt gataataaag tagtttattg tgaataaaag ttttcaaatg ggagtgtata
    17461 ttcaaacgat actcctttag gttcgtaata aagggggctg tatcatgaca ggagagacag
    17521 ttgtatataa aaatgaaatg aatttagttc cattaaggcg atttacagct actgaaatta
    17581 atttattttt tgcgatgtgt aataaattaa aagagcaaga tactaataca ttacgtttgt
    17641 cttttgatga attaaaaaaa ttaagtaatt atagtccaga aacacgtaat attaatagat
    17701 ttgctaatga tttagataat gtgtataaaa agatgttgaa tttaaccatt agatatgaag
    17761 atgatgatgt aatagaaaga ttcgttttat tcaatcatta tagaatccat aagcgagaac
    17821 aatatctaga aatatcaact tcttcaaatt taaaacacat attgaattca attactaata
    17881 attttacaaa atttgaatta aaagaaatga ctaggcttaa atctacatat tctaaaaata
    17941 tgtttagatt acttaaacaa tacaaacata caggctattt aaaaattcac atcgatgatt
    18001 ttaaaaatcg tttagatata cctaaatctt atcgtatgac tgatattaac aaaaatgttt
    18061 ttaaacctat tattattgaa ttaggttcta tatttaataa tcttactatc aataaaatca
    18121 aagcgaaaaa agggcgtaaa attgaatgga tagagttcac atttgacgct gagaaacgca
    18181 ttcataacaa acgacaacct caaatgtcca agatagataa atcaagacaa tatgtgaggc
    18241 gtgaaaagac gcctaaatgg ttagaagaac ggtcatatga aaagcaacct caaaaagatt
    18301 acgacccaca attagagaaa gaacgagaga attttttaaa acaacttgaa ctgaattggg
    18361 aataacgcaa cttttcgttg cgagttggtt aactaaaagt atcgtgactt ttcaattatt
    18421 ttaattatag tttgagttaa ggagtgattt tatgaaatta gctgaagcta taaaagaaca
    18481 gagagaatta aaaggatggt ctcaagagga attagccaat atattaaagg tttccagaca
    18541 aagtgtttct aaatgggaaa gtgccaaaaa ctatccttcc ttagatattt tgattgctat
    18601 gagcgattta ttcggaatct ctttagagca tttaattaaa ggagatgcac gttttaaaaa
    18661 agttatttta gagggaaatt atagagagag taacagggta ccttccatag ttgattttct
    18721 ttatgatttt tggtggcttt tctttcctgt tgccggattt ttagtttggg ccattcattc
    18781 gttcatgggt tgattttaaa atcaaaattt aatgcacatc aggcaatatg aaaaaggaaa
    18841 gtatttttac gacaccttga aatgtattga taagaataga agagtttaca aaaggcaata
    18901 tgaaatatat actaaaagta acaagaattt cgtttgtaaa cgcaccaaac cccgaactat
    18961 tcgccgtagt tcggggtttt ttggtgaatt ggctttaatc gtctatatta ttttttatca
    19021 cgtttatgga gctaataacc aaataacgta acgagacaac cactgattgc cgttgtcatg
    19081 atgtgaacaa gaatttcatt cataaatcgc acctcctctc tacgccgaat tgacatctga
    19141 gagatagacg accttcacat tataacaaat ctctccttcc atgaaataac atgaatatta
    19201 ataaatgttg ttatatatta aagataaata aatataaggg tgggattgat aatgtttggc
    19261 gatcaaagac aagaagctac aaaatatgtc attaaagaag gttatcaaga tatttatttc
    19321 ctcaacaaaa acggggaatg gtattatttc gaagtaagaa gtgtctggcg tggcaaacat
    19381 attattagag ttaaagatgg tttgttaggt tggagaaaag aaatagtaac tgaataaaga
    19441 tacaaaatac ttgccaatgc aaaagaatga aaacgcgtca aatcaacgat gtataaaaga
    19501 tgcataaaag ataatgaaag taaaaagcca taaatgccgt catataggca tttatggctt
    19561 tttgtttggt taatataatc gatgattatc agaagttcaa ttcacataaa aaagcacggc
    19621 taatttatga acttgtcgta cctttttaat taaaattagt taccaaatag acgactccaa
    19681 aacccctttt tagcttccgt ttcttcactc ttttcttcta tatcactatc attgtcattt
    19741 ggattagctg aaacgtcttc tctttgtgct tcttcacctt gtgtactttc gtcgctttga
    19801 cttccagata tatccttata ttgaatctct gtttctttag tttcttcctg atctcggtta
    19861 atatttacag gactagtagt atatgttttt tcttgtgcat caacgttttg tctagcgtta
    19921 gtagatgtat caaagggata attaaggtgt ttttcttttt ctaattgatt ttctaatttt
    19981 tgaatctttt tattactctc taatgccaga acttgctgat tctcaagcaa ttttgatctt
    20041 tcttgcaact cttttctgta aaattcaatt gttgtttcat gacgactttc ttgtttttca
    20101 atttgattat ttaaagtatt aatctgattt ttaagtattt ctactatttg agcatcgctt
    20161 ttagattcaa aattaatatt atccgactct tttttttgtt tgtcttcagt agcactttca
    20221 aaaccatatt tctttttatt ttcatttact ctttgaatga ttttttctat atcggtatct
    20281 tctttaataa acgaagtgtt ttcctgtttt attgtctcta tattcaatct cttaatatta
    20341 ttaaaaatcg tttgcttact aacccctaat tcttcactca acctttttac acttttcata
    20401 tcatcaaccc tttataaaat gtttataaac ccttagtaaa atattataag cttataacga
    20461 ttaataataa agtaatcgac aaataaaaat ataaattcgc caaccttttt atcaagggtt
    20521 tgtaaagggt ttatggtgtt tatcaaaggt ttataaacct atattttccc ttttttaagt
    20581 ttattcaaag tgctataata accttaatat aacaaaaacc ccaactactg gtaatagtcg
    20641 gggt
//
