LOCUS       NZ_CP086517             6647 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Escherichia coli strain Ec-050-T0 plasmid pEc-050-T0_7, complete
            sequence.
ACCESSION   NZ_CP086517
VERSION     NZ_CP086517.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22108721
            Assembly: GCF_022759805.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Genome plasticity in E. coli and K. pneumoniae: SNV accumulation
            over time
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 271x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022759805.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 04:49:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,277
            CDSs (total)                      :: 5,155
            Genes (coding)                    :: 4,886
            CDSs (with protein)               :: 4,886
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 269
            CDSs (without protein)            :: 269
            Pseudo Genes (ambiguous residues) :: 0 of 269
            Pseudo Genes (frameshifted)       :: 109 of 269
            Pseudo Genes (incomplete)         :: 173 of 269
            Pseudo Genes (internal stop)      :: 53 of 269
            Pseudo Genes (multiple problems)  :: 58 of 269
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086517.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec-050-T0"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc-050-T0_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            260..418
                     /locus_tag="LNN50_RS26470"
     CDS             260..418
                     /locus_tag="LNN50_RS26470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            916..1143
                     /locus_tag="LNN50_RS26115"
                     /old_locus_tag="LNN50_26120"
     CDS             916..1143
                     /locus_tag="LNN50_RS26115"
                     /old_locus_tag="LNN50_26120"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            complement(1140..2708)
                     /gene="cea"
                     /locus_tag="LNN50_RS26120"
                     /old_locus_tag="LNN50_26125"
     CDS             complement(1140..2708)
                     /gene="cea"
                     /locus_tag="LNN50_RS26120"
                     /old_locus_tag="LNN50_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2968..3405
                     /locus_tag="LNN50_RS26125"
                     /old_locus_tag="LNN50_26130"
     CDS             2968..3405
                     /locus_tag="LNN50_RS26125"
                     /old_locus_tag="LNN50_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            3486..3905
                     /locus_tag="LNN50_RS26130"
                     /old_locus_tag="LNN50_26135"
     CDS             3486..3905
                     /locus_tag="LNN50_RS26130"
                     /old_locus_tag="LNN50_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(4223..4456)
                     /locus_tag="LNN50_RS26135"
                     /old_locus_tag="LNN50_26140"
     CDS             complement(4223..4456)
                     /locus_tag="LNN50_RS26135"
                     /old_locus_tag="LNN50_26140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(4463..4981)
                     /locus_tag="LNN50_RS26140"
                     /old_locus_tag="LNN50_26145"
     CDS             complement(4463..4981)
                     /locus_tag="LNN50_RS26140"
                     /old_locus_tag="LNN50_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<5019..5717)
                     /locus_tag="LNN50_RS26475"
                     /old_locus_tag="LNN50_26150"
                     /pseudo=""
     CDS             complement(<5019..5717)
                     /locus_tag="LNN50_RS26475"
                     /old_locus_tag="LNN50_26150"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(5659..5982)
                     /locus_tag="LNN50_RS26150"
                     /old_locus_tag="LNN50_26155"
     CDS             complement(5659..5982)
                     /locus_tag="LNN50_RS26150"
                     /old_locus_tag="LNN50_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            6046..6237
                     /locus_tag="LNN50_RS26155"
                     /old_locus_tag="LNN50_26160"
     CDS             6046..6237
                     /locus_tag="LNN50_RS26155"
                     /old_locus_tag="LNN50_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(6323..6410)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..369
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.297"
                     /coverage="90.0"
                     /evalue="1.6199999999999998e-176"
     Typing          6421..6639
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.665"
                     /coverage="99.0"
                     /evalue="7.14e-87"
     Typing          complement(4120..5669)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
       61 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      181 gggaagcgtg gcgctttctc atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt
      241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      301 cggtaactat cgtcttgagt ccaacccggt aaggcacgcc ttaacgccac tggcagcagc
      361 cactggtaac cggattagca gagcgatgat ggcacaaacg gtgctacaga gttcttgaag
      421 tagtggcccg actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
      481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgttggt
      541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
      601 gatcctttga tcttttctac tgaaccgcga tccccgtcag tttagaagag gaggatggtg
      661 cgatggtccc tccctgaaca tcacgtatat agttagcctg acatccaaca aggaggttta
      721 tcgcgaatat tcccacaaaa aatcttttcc tcataactcg atccttataa aatgaaaaga
      781 atatatggcg aggtttaatt tatgagctta agatactaca taaaaaatat tttatttggc
      841 ctgtactgca cacttatata tatatacctt ataacaaaaa acagcgaagg gtattatttc
      901 cttgtgtcag ataagatgct atatgcaata gtgataagca ctattctatg tccatattca
      961 aaatatgcta ttgaacacat agcttttaac ttcataaaga aagatttttt cgaaagaaga
     1021 aaaaacctaa ataacgcccc cgtagcaaaa ttaaacctat ttatgctata taatctactt
     1081 tgtttggtcc tagcaatccc atttggattg ctaggacttt ttatatcaat aaagaataat
     1141 taaatcccta acacctcatt tatagtatta agtttattct tatcaatata ggagcataga
     1201 attcctgtaa caatagcaat accccaaata cctaatgtag ttccagcaag caagctaaaa
     1261 agtaaagcaa caacataact cacccctgca tctgcagctt tcttctctaa tgtaagaaat
     1321 agtggcttcc agtcacctgt atccttaatt tttaggatat cagataccac atcatatcca
     1381 aaagaaacat gccccgtaat cttcaagtac ttggcaaact gatctaaatg tttagcccag
     1441 tcatcatact tcaccgatgc caacgcatta aaaatagcat cacgatcggc tttgctgaat
     1501 ttcttattta aaacatcctt gtatttttca aaagcagcga gagcttcatt cacattgccg
     1561 attttcttac ctttagactt atcagcaagt tcctgtgcca ttttcgaata tttttcacca
     1621 tatttttcag tcagcgtttg ataaaagcta actgttgcat caacagcatc cttaatctgt
     1681 gaattaagga gattattctg tgcttttttc aaattttctt cagcttcatg aacacgagcg
     1741 ataccggcat tacgattatt actgacctga gaaatagcct tctggatctg agttatatca
     1801 gcatttatcc ggttaatacg tgtttctgat gctgttacct gtttttgttt ttcttctcta
     1861 atcttaccgg ccccaacccg tcgtctggtt gcttcaaaaa aaggacggtt ctgaagcgga
     1921 tcattggctc ttggtgatag ttttttgacc agctcatcca gttctttata tttagcggat
     1981 gcctgagcca gttcatttcg ttttccagcg agcgttttca tttctgcatc acgggcatgg
     2041 atactggagc ttaaacgaga attgagagtc ttaatctctc catccatttt caccacttca
     2101 gattgtgcag cagaaagttt tttttgggcg atctcaacag ctttagcttc ttcactcaat
     2161 gcagccagtc gtttctcttc agcttcagcc agtttcaact ggcgttctgt ttcagccttc
     2221 tcccgttcaa tctctttacg tcgttgttct gcttcctgaa aagccttttc tgctgcttcc
     2281 gcttctttac gggctttttc ttctgctttc gcaaggcgca aacgctctgc ttccgcctgc
     2341 atagctgcat tattagcatg agcaagctct gttgctgaag gcgtacgtga ggcattgtga
     2401 cgaagagcct cattcacgat atccttcagg cgctgagtca gcgcatccct gtttgccttt
     2461 gctttcgcct gtgcttccgc tgcagctttt gcccgggcag cctgctctgc ctgtgttttc
     2521 tttaattgag cagtagacca tttagcagtt gcatgaatag ctgcagaact ttcactttta
     2581 ctgcctcctt ttccacctcc gccgccagag ccactcccgt caggagtacc attcaaaaga
     2641 gtaataatta cctgtccctt atcatcataa ggaacaccat ctttatagta cgctaccgcg
     2701 gtttccatta taaaatcctc tttgactttt aaaacaataa gttaaaaata aatactgtac
     2761 atataaccac tggttttata tacagcataa aagctacgcc gctgcatttt ccctgtcaag
     2821 actgtggact tccatttttg tgaaaacgat caaaaaaaca gtctttcaca ccacgcgcta
     2881 ttctcgcccg atgccacaaa aaccagcaca aacattaccg ttctcagacc tcattatgtt
     2941 ttactgaaac tatgagatga gacatctatg ggacactgtc actttatggc atggcacaca
     3001 ctccgggacg cactaaaaat gacaggcaga tcgcgttcac agttttaccg tgatatgcgc
     3061 gcaggccttg tcagttaccg taccggcagg gacggacgac gggagtttga aaccagtgaa
     3121 ctgatccggg catacggcga attaaagcag aatgagacac cagaaaggca cagtgaggga
     3181 catgcagaaa atccacatga tcagcagaca gaacgcattc tccgggagct gaatgagctg
     3241 aaacaatgcc tgacgctgat gcttgaggat aaacaggcac aggatatgga tcgcagacgc
     3301 caggaagcag aacgggaaca gctacaaaat gagatagccc agctcaggca ggcactggaa
     3361 ctggaaaaga aacggggatt ctggtccagg ttgttcggtc gctgaacgct gtcagagact
     3421 gatgataaaa tagtcttcgg ataataactc accgagaata aatactttaa ggtagggaga
     3481 cactcatgag acgtaccgga aacaaacttt gtcttatcgc catgataaca gcaacagtag
     3541 ctctcacagc ctgtacccca aagggcagcg tggaacaaca tacccggcat tacgtatatg
     3601 cttctgatga cggttttgat cccaactttt ccacccaaaa agccgacaca acacgaatga
     3661 tggtgccttt ttttcggcag ttctgggata tgggagctaa agataaagcg acaggaaaat
     3721 cacggagtga tgtgcaacaa cgcattcagc agtttcacag ccaggaattt ttaaactcac
     3781 tccggggcac aactcaattt gcgggtactg attaccgcag caaagacctt accccgaaaa
     3841 aatccaggct gctggctgac acgatttctg cggtttatct cgatggctac gagggcagac
     3901 agtaagtgga tttaccataa tcccttaatt gtacgcaccg ctaaaacgcg ttcagcgcga
     3961 tcacggcagc agacaggtaa aaatggcaac aaaccacccg aaaaactgcc gcgatcgcgc
     4021 ctgataaatt ttaaccgtat gaatacctat gcaaccagag ggtacaggcc acattacccc
     4081 cacttaatcc actgaagctg ccatttttca tggtttcacc atcccagcga agggccatcc
     4141 agcgtgcgtt cctgtatttc cggctgacgc tcccgttcca gggataacac atgttcgcgc
     4201 tcctgtatca gccgttcctc tcttatctcc agttctcgct gtataactgg ctcaagcgtt
     4261 ctgtctgctc gctcaagtgc tgcacctgct gactcaactg catgacccgc tcgttcagca
     4321 tcgcgttgtc ccgttgcgta agcgaaaaca tcttctgcaa ttccacgaag gcgctctccc
     4381 attcgctcag ccgctgcata tagtcctgtt gcagctgctc taaggcgttc agcaaatgtg
     4441 tttccagctc tgtcactctg tgtcactcct tcagatgtac ccactctttc ccctgaaagg
     4501 gaatcacctc cgctgatttc ccgtacggaa ggacaaggaa tttcctgttc ccgtcctgca
     4561 caaactccac gccccatgtc ttcgcgttca gtttctgcaa tgtctcttcc tgcttcctga
     4621 tttcttccag gttcgcctgt atcctccctc caagatacca gagcgtcccg ccactcgcgg
     4681 taaacaggag aaagactatc cccagtaaca tcatgcccgt attccctgcc agctttaaca
     4741 cgtccctcct gtgctgcatc atcgcctctt tcaccccttc ccggtgtttt tccagcgatt
     4801 cctctgtcga ggctgtgaac agggctatag cgtctctgat tttcgtctcg tttgatgtca
     4861 cagcctcgct tacagattcg ccgagcctcc tgaactcgtt gttcagcatt ttctctgtag
     4921 attcggctct ctctttcagc tttttctcga actccgcgcc cgtctgcaaa agattgctca
     4981 taaaatgctc ctttcagcct gatattcttc ccgccgttcg gatctgcaat gctgatactg
     5041 cttcgcgtca ccctgaccac ttccagcccc gcctcagtga gcgcctgaat cacatcctga
     5101 cggcctttta tctctccggc atggtaaagt gcatctatac ctcgcgtgac gccctcagca
     5161 agcgcctgtt tcgtttcagg caggttatca gggagtgtca gcgtcctgcg gttctccggg
     5221 gcgttcgggt catgcagccc gtaatggtga ttcaccagcg tctgccaggc atcaattcta
     5281 ggcctgtctg cgcggtcgta gtacggctgg aggcgttttc cggtctgtag ctccatgttc
     5341 ggaatgacaa aattcagctc aagccgtccc ttgtcctggt gctccaccca caggatgctg
     5401 tactgatttt tttcgagacc gggcatcagt acacgctcaa agctcgccat cactttttca
     5461 cgtcctcccg gcggcagctc cttctccgcg aacgacagaa caccggacgt gtatttcttc
     5521 gcaaatggcg tggcatcgat gagttcccgg acttcttccg gattaccctg aagcaccgtt
     5581 gcgccttcgc ggttacgctc cctccccagc aggtaatcaa ccggaccact gccaccacct
     5641 tttcccctgg catgaaattt aactatcatc ccgcgccccc tgttccctga cggccagacg
     5701 cagccggcgc agctcatccc cgatggccat cagtgcggcc accacctgaa cccggtcacc
     5761 ggaagaccac tgcccgctgt tcaccttacg ggctgtctga ttcaggttat ttccgatggc
     5821 ggccagctga cgcagtaacg gcggtgccag tgtcggcagt tttccggaac gggcaaccgg
     5881 ctcccccagg cagacccgcc gcatccatac cgccagttgt ttaccctcac agcgttcaag
     5941 taaccgggca tgttcatcat cagtaacccg tattgtgagc atcctctcgc gtttcatcgg
     6001 tatcattacc ccatgaacag aaatccccct tacacggagg catcagtgac taaacaggaa
     6061 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct gctggagaag
     6121 ctcaacgaac tggacgcaga tgaacaggcc gatatttgtg aatcgcttca cgaccacgcc
     6181 gatgagcttt accgcagctg cctcgcacgt ttcggggatg acggtgaaaa cctctgatac
     6241 atgcagctcc cggagacggt cacagcttgt ctgtgagcgg atgccgggag ctgacaagcc
     6301 cgtcagggcg cgtcagcagg ttttagcggg tgtcggggcg cagccctgac ccagtcacgt
     6361 agcgatagcg gagtgtatac tggcttaacc atgcggcatc agtgcggatt gtatgaaaag
     6421 tacgccatgc cgggtgtgaa atgccgcaca gatgcgtaag gagaaaatgc acgtccaggc
     6481 gcttttccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg cggcgagcgg
     6541 tactgactca cacaaaaacg gtaacacagt tatccacaga atcaggggat aaggccggaa
     6601 agaacatgtg agcaaaagac caggaacagg aagaaggcca cgtagca
//
