LOCUS       NZ_CP086507             6647 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Escherichia coli strain Ec-050-T5-MAC plasmid pEc-050-T5-MAC_7,
            complete sequence.
ACCESSION   NZ_CP086507
VERSION     NZ_CP086507.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22108722
            Assembly: GCF_022759725.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Genome plasticity in E. coli and K. pneumoniae: SNV accumulation
            over time
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 275x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022759725.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 05:24:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,158
            Genes (coding)                    :: 4,888
            CDSs (with protein)               :: 4,888
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 108 of 270
            Pseudo Genes (incomplete)         :: 174 of 270
            Pseudo Genes (internal stop)      :: 53 of 270
            Pseudo Genes (multiple problems)  :: 57 of 270
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086507.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec-050-T5-MAC"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc-050-T5-MAC_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(6298..6647,1..1219))
                     /gene="cea"
                     /locus_tag="LNN49_RS26135"
                     /old_locus_tag="LNN49_26140"
     CDS             complement(join(6298..6647,1..1219))
                     /gene="cea"
                     /locus_tag="LNN49_RS26135"
                     /old_locus_tag="LNN49_26140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            1479..1916
                     /locus_tag="LNN49_RS26140"
                     /old_locus_tag="LNN49_26145"
     CDS             1479..1916
                     /locus_tag="LNN49_RS26140"
                     /old_locus_tag="LNN49_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1997..2416
                     /locus_tag="LNN49_RS26145"
                     /old_locus_tag="LNN49_26150"
     CDS             1997..2416
                     /locus_tag="LNN49_RS26145"
                     /old_locus_tag="LNN49_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(2734..2967)
                     /locus_tag="LNN49_RS26150"
                     /old_locus_tag="LNN49_26155"
     CDS             complement(2734..2967)
                     /locus_tag="LNN49_RS26150"
                     /old_locus_tag="LNN49_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2974..3492)
                     /locus_tag="LNN49_RS26155"
                     /old_locus_tag="LNN49_26160"
     CDS             complement(2974..3492)
                     /locus_tag="LNN49_RS26155"
                     /old_locus_tag="LNN49_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<3530..4228)
                     /locus_tag="LNN49_RS26485"
                     /old_locus_tag="LNN49_26165"
                     /pseudo=""
     CDS             complement(<3530..4228)
                     /locus_tag="LNN49_RS26485"
                     /old_locus_tag="LNN49_26165"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4170..4493)
                     /locus_tag="LNN49_RS26165"
                     /old_locus_tag="LNN49_26170"
     CDS             complement(4170..4493)
                     /locus_tag="LNN49_RS26165"
                     /old_locus_tag="LNN49_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4557..4748
                     /locus_tag="LNN49_RS26170"
                     /old_locus_tag="LNN49_26175"
     CDS             4557..4748
                     /locus_tag="LNN49_RS26170"
                     /old_locus_tag="LNN49_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5418..5576
                     /locus_tag="LNN49_RS26490"
     CDS             5418..5576
                     /locus_tag="LNN49_RS26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5775..5912)
                     /locus_tag="LNN49_RS26175"
                     /old_locus_tag="LNN49_26180"
     CDS             complement(5775..5912)
                     /locus_tag="LNN49_RS26175"
                     /old_locus_tag="LNN49_26180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            6074..6301
                     /locus_tag="LNN49_RS26180"
                     /old_locus_tag="LNN49_26185"
     CDS             6074..6301
                     /locus_tag="LNN49_RS26180"
                     /old_locus_tag="LNN49_26185"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_230498631.1"
                     /translation="MLYAIVISTILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAK
                     LNLFMLYNLLCLVLAIPFGLLGLFISIKNN"
                     /name="colicin E1 immunity protein"
     Typing          complement(4834..4921)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          4932..5490
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2631..4180)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctttgctgaa tttcttattt aaaacatcct tgtatttttc aaaagcagcg agagcttcat
       61 tcacattgcc gattttctta cctttagact tatcagcaag ttcctgtgcc attttcgaat
      121 atttttcacc atatttttca gtcagcgttt gataaaagct aactgttgca tcaacagcat
      181 ccttaatctg tgaattaagg agattattct gtgctttttt caaattttct tcagcttcat
      241 gaacacgagc gataccggca ttacgattat tactgacctg agaaatagcc ttctggatct
      301 gagttatatc agcatttatc cggttaatac gtgtttctga tgctgttacc tgtttttgtt
      361 tttcttctct aatcttaccg gccccaaccc gtcgtctggt tgcttcaaaa aaaggacggt
      421 tctgaagcgg atcattggct cttggtgata gttttttgac cagctcatcc agttctttat
      481 atttagcgga tgcctgagcc agttcatttc gttttccagc gagcgttttc atttctgcat
      541 cacgggcatg gatactggag cttaaacgag aattgagagt cttaatctct ccatccattt
      601 tcaccacttc agattgtgca gcagaaagtt ttttttgggc gatctcaaca gctttagctt
      661 cttcactcaa tgcagccagt cgtttctctt cagcttcagc cagtttcaac tggcgttctg
      721 tttcagcctt ctcccgttca atctctttac gtcgttgttc tgcttcctga aaagcctttt
      781 ctgctgcttc cgcttcttta cgggcttttt cttctgcttt cgcaaggcgc aaacgctctg
      841 cttccgcctg catagctgca ttattagcat gagcaagctc tgttgctgaa ggcgtacgtg
      901 aggcattgtg acgaagagcc tcattcacga tatccttcag gcgctgagtc agcgcatccc
      961 tgtttgcctt tgctttcgcc tgtgcttccg ctgcagcttt tgcccgggca gcctgctctg
     1021 cctgtgtttt ctttaattga gcagtagacc atttagcagt tgcatgaata gctgcagaac
     1081 tttcactttt actgcctcct tttccacctc cgccgccaga gccactcccg tcaggagtac
     1141 cattcaaaag agtaataatt acctgtccct tatcatcata aggaacacca tctttatagt
     1201 acgctaccgc ggtttccatt ataaaatcct ctttgacttt taaaacaata agttaaaaat
     1261 aaatactgta catataacca ctggttttat atacagcata aaagctacgc cgctgcattt
     1321 tccctgtcaa gactgtggac ttccattttt gtgaaaacga tcaaaaaaac agtctttcac
     1381 accacgcgct attctcgccc gatgccacaa aaaccagcac aaacattacc gttctcagac
     1441 ctcattatgt tttactgaaa ctatgagatg agacatctat gggacactgt cactttatgg
     1501 catggcacac actccgggac gcactaaaaa tgacaggcag atcgcgttca cagttttacc
     1561 gtgatatgcg cgcaggcctt gtcagttacc gtaccggcag ggacggacga cgggagtttg
     1621 aaaccagtga actgatccgg gcatacggcg aattaaagca gaatgagaca ccagaaaggc
     1681 acagtgaggg acatgcagaa aatccacatg atcagcagac agaacgcatt ctccgggagc
     1741 tgaatgagct gaaacaatgc ctgacgctga tgcttgagga taaacaggca caggatatgg
     1801 atcgcagacg ccaggaagca gaacgggaac agctacaaaa tgagatagcc cagctcaggc
     1861 aggcactgga actggaaaag aaacggggat tctggtccag gttgttcggt cgctgaacgc
     1921 tgtcagagac tgatgataaa atagtcttcg gataataact caccgagaat aaatacttta
     1981 aggtagggag acactcatga gacgtaccgg aaacaaactt tgtcttatcg ccatgataac
     2041 agcaacagta gctctcacag cctgtacccc aaagggcagc gtggaacaac atacccggca
     2101 ttacgtatat gcttctgatg acggttttga tcccaacttt tccacccaaa aagccgacac
     2161 aacacgaatg atggtgcctt tttttcggca gttctgggat atgggagcta aagataaagc
     2221 gacaggaaaa tcacggagtg atgtgcaaca acgcattcag cagtttcaca gccaggaatt
     2281 tttaaactca ctccggggca caactcaatt tgcgggtact gattaccgca gcaaagacct
     2341 taccccgaaa aaatccaggc tgctggctga cacgatttct gcggtttatc tcgatggcta
     2401 cgagggcaga cagtaagtgg atttaccata atcccttaat tgtacgcacc gctaaaacgc
     2461 gttcagcgcg atcacggcag cagacaggta aaaatggcaa caaaccaccc gaaaaactgc
     2521 cgcgatcgcg cctgataaat tttaaccgta tgaataccta tgcaaccaga gggtacaggc
     2581 cacattaccc ccacttaatc cactgaagct gccatttttc atggtttcac catcccagcg
     2641 aagggccatc cagcgtgcgt tcctgtattt ccggctgacg ctcccgttcc agggataaca
     2701 catgttcgcg ctcctgtatc agccgttcct ctcttatctc cagttctcgc tgtataactg
     2761 gctcaagcgt tctgtctgct cgctcaagtg ctgcacctgc tgactcaact gcatgacccg
     2821 ctcgttcagc atcgcgttgt cccgttgcgt aagcgaaaac atcttctgca attccacgaa
     2881 ggcgctctcc cattcgctca gccgctgcat atagtcctgt tgcagctgct ctaaggcgtt
     2941 cagcaaatgt gtttccagct ctgtcactct gtgtcactcc ttcagatgta cccactcttt
     3001 cccctgaaag ggaatcacct ccgctgattt cccgtacgga aggacaagga atttcctgtt
     3061 cccgtcctgc acaaactcca cgccccatgt cttcgcgttc agtttctgca atgtctcttc
     3121 ctgcttcctg atttcttcca ggttcgcctg tatcctccct ccaagatacc agagcgtccc
     3181 gccactcgcg gtaaacagga gaaagactat ccccagtaac atcatgcccg tattccctgc
     3241 cagctttaac acgtccctcc tgtgctgcat catcgcctct ttcacccctt cccggtgttt
     3301 ttccagcgat tcctctgtcg aggctgtgaa cagggctata gcgtctctga ttttcgtctc
     3361 gtttgatgtc acagcctcgc ttacagattc gccgagcctc ctgaactcgt tgttcagcat
     3421 tttctctgta gattcggctc tctctttcag ctttttctcg aactccgcgc ccgtctgcaa
     3481 aagattgctc ataaaatgct cctttcagcc tgatattctt cccgccgttc ggatctgcaa
     3541 tgctgatact gcttcgcgtc accctgacca cttccagccc cgcctcagtg agcgcctgaa
     3601 tcacatcctg acggcctttt atctctccgg catggtaaag tgcatctata cctcgcgtga
     3661 cgccctcagc aagcgcctgt ttcgtttcag gcaggttatc agggagtgtc agcgtcctgc
     3721 ggttctccgg ggcgttcggg tcatgcagcc cgtaatggtg attcaccagc gtctgccagg
     3781 catcaattct aggcctgtct gcgcggtcgt agtacggctg gaggcgtttt ccggtctgta
     3841 gctccatgtt cggaatgaca aaattcagct caagccgtcc cttgtcctgg tgctccaccc
     3901 acaggatgct gtactgattt ttttcgagac cgggcatcag tacacgctca aagctcgcca
     3961 tcactttttc acgtcctccc ggcggcagct ccttctccgc gaacgacaga acaccggacg
     4021 tgtatttctt cgcaaatggc gtggcatcga tgagttcccg gacttcttcc ggattaccct
     4081 gaagcaccgt tgcgccttcg cggttacgct ccctccccag caggtaatca accggaccac
     4141 tgccaccacc ttttcccctg gcatgaaatt taactatcat cccgcgcccc ctgttccctg
     4201 acggccagac gcagccggcg cagctcatcc ccgatggcca tcagtgcggc caccacctga
     4261 acccggtcac cggaagacca ctgcccgctg ttcaccttac gggctgtctg attcaggtta
     4321 tttccgatgg cggccagctg acgcagtaac ggcggtgcca gtgtcggcag ttttccggaa
     4381 cgggcaaccg gctcccccag gcagacccgc cgcatccata ccgccagttg tttaccctca
     4441 cagcgttcaa gtaaccgggc atgttcatca tcagtaaccc gtattgtgag catcctctcg
     4501 cgtttcatcg gtatcattac cccatgaaca gaaatccccc ttacacggag gcatcagtga
     4561 ctaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc
     4621 tgctggagaa gctcaacgaa ctggacgcag atgaacaggc cgatatttgt gaatcgcttc
     4681 acgaccacgc cgatgagctt taccgcagct gcctcgcacg tttcggggat gacggtgaaa
     4741 acctctgata catgcagctc ccggagacgg tcacagcttg tctgtgagcg gatgccggga
     4801 gctgacaagc ccgtcagggc gcgtcagcag gttttagcgg gtgtcggggc gcagccctga
     4861 cccagtcacg tagcgatagc ggagtgtata ctggcttaac catgcggcat cagtgcggat
     4921 tgtatgaaaa gtacgccatg ccgggtgtga aatgccgcac agatgcgtaa ggagaaaatg
     4981 cacgtccagg cgcttttccg cttcctcgct cactgactcg ctacgctcgg tcgttcgact
     5041 gcggcgagcg gtactgactc acacaaaaac ggtaacacag ttatccacag aatcagggga
     5101 taaggccgga aagaacatgt gagcaaaaga ccaggaacag gaagaaggcc acgtagcagg
     5161 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     5221 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     5281 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     5341 gaagcgtggc gctttctcat agctcacgct gttggtatct cagttcggtg taggtcgttc
     5401 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5461 gtaactatcg tcttgagtcc aacccggtaa ggcacgcctt aacgccactg gcagcagcca
     5521 ctggtaaccg gattagcaga gcgatgatgg cacaaacggt gctacagagt tcttgaagta
     5581 gtggcccgac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     5641 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgttggtag
     5701 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     5761 tcctttgatc ttttctactg aaccgcgatc cccgtcagtt tagaagagga ggatggtgcg
     5821 atggtccctc cctgaacatc acgtatatag ttagcctgac atccaacaag gaggtttatc
     5881 gcgaatattc ccacaaaaaa tcttttcctc ataactcgat ccttataaaa tgaaaagaat
     5941 atatggcgag gtttaattta tgagcttaag atactacata aaaaatattt tatttggcct
     6001 gtactgcaca cttatatata tataccttat aacaaaaaac agcgaagggt attatttcct
     6061 tgtgtcagat aagatgctat atgcaatagt gataagcact attctatgtc catattcaaa
     6121 atatgctatt gaacacatag cttttaactt cataaagaaa gattttttcg aaagaagaaa
     6181 aaacctaaat aacgcccccg tagcaaaatt aaacctattt atgctatata atctactttg
     6241 tttggtccta gcaatcccat ttggattgct aggacttttt atatcaataa agaataatta
     6301 aatccctaac acctcattta tagtattaag tttattctta tcaatatagg agcatagaat
     6361 tcctgtaaca atagcaatac cccaaatacc taatgtagtt ccagcaagca agctaaaaag
     6421 taaagcaaca acataactca cccctgcatc tgcagctttc ttctctaatg taagaaatag
     6481 tggcttccag tcacctgtat ccttaatttt taggatatca gataccacat catatccaaa
     6541 agaaacatgc cccgtaatct tcaagtactt ggcaaactga tctaaatgtt tagcccagtc
     6601 atcatacttc accgatgcca acgcattaaa aatagcatca cgatcgg
//
