LOCUS       NZ_CP086497             6647 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Escherichia coli strain Ec-050-T5-ESBL plasmid pEc-050-T5-ESBL_7,
            complete sequence.
ACCESSION   NZ_CP086497
VERSION     NZ_CP086497.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22108723
            Assembly: GCF_022759745.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Genome plasticity in E. coli and K. pneumoniae: SNV accumulation
            over time
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 225x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022759745.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 04:58:31
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,273
            CDSs (total)                      :: 5,151
            Genes (coding)                    :: 4,884
            CDSs (with protein)               :: 4,884
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 267
            CDSs (without protein)            :: 267
            Pseudo Genes (ambiguous residues) :: 0 of 267
            Pseudo Genes (frameshifted)       :: 107 of 267
            Pseudo Genes (incomplete)         :: 174 of 267
            Pseudo Genes (internal stop)      :: 53 of 267
            Pseudo Genes (multiple problems)  :: 59 of 267
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086497.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec-050-T5-ESBL"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc-050-T5-ESBL_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(173..1741)
                     /gene="cea"
                     /locus_tag="LNN51_RS26110"
                     /old_locus_tag="LNN51_26115"
     CDS             complement(173..1741)
                     /gene="cea"
                     /locus_tag="LNN51_RS26110"
                     /old_locus_tag="LNN51_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            2001..2438
                     /locus_tag="LNN51_RS26115"
                     /old_locus_tag="LNN51_26120"
     CDS             2001..2438
                     /locus_tag="LNN51_RS26115"
                     /old_locus_tag="LNN51_26120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2519..2938
                     /locus_tag="LNN51_RS26120"
                     /old_locus_tag="LNN51_26125"
     CDS             2519..2938
                     /locus_tag="LNN51_RS26120"
                     /old_locus_tag="LNN51_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(3256..3489)
                     /locus_tag="LNN51_RS26125"
                     /old_locus_tag="LNN51_26130"
     CDS             complement(3256..3489)
                     /locus_tag="LNN51_RS26125"
                     /old_locus_tag="LNN51_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3496..4014)
                     /locus_tag="LNN51_RS26130"
                     /old_locus_tag="LNN51_26135"
     CDS             complement(3496..4014)
                     /locus_tag="LNN51_RS26130"
                     /old_locus_tag="LNN51_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4052..4750)
                     /locus_tag="LNN51_RS26455"
                     /old_locus_tag="LNN51_26140"
                     /pseudo=""
     CDS             complement(<4052..4750)
                     /locus_tag="LNN51_RS26455"
                     /old_locus_tag="LNN51_26140"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4692..5015)
                     /locus_tag="LNN51_RS26140"
                     /old_locus_tag="LNN51_26145"
     CDS             complement(4692..5015)
                     /locus_tag="LNN51_RS26140"
                     /old_locus_tag="LNN51_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5079..5270
                     /locus_tag="LNN51_RS26145"
                     /old_locus_tag="LNN51_26150"
     CDS             5079..5270
                     /locus_tag="LNN51_RS26145"
                     /old_locus_tag="LNN51_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5940..6098
                     /locus_tag="LNN51_RS26460"
     CDS             5940..6098
                     /locus_tag="LNN51_RS26460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(6297..6434)
                     /locus_tag="LNN51_RS26150"
                     /old_locus_tag="LNN51_26155"
     CDS             complement(6297..6434)
                     /locus_tag="LNN51_RS26150"
                     /old_locus_tag="LNN51_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     Typing          complement(5356..5443)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5454..6012
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.722"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3153..4702)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ctattgaaca catagctttt aacttcataa agaaagattt tttcgaaaga agaaaaaacc
       61 taaataacgc ccccgtagca aaattaaacc tatttatgct atataatcta ctttgtttgg
      121 tcctagcaat cccatttgga ttgctaggac tttttatatc aataaagaat aattaaatcc
      181 ctaacacctc atttatagta ttaagtttat tcttatcaat ataggagcat agaattcctg
      241 taacaatagc aataccccaa atacctaatg tagttccagc aagcaagcta aaaagtaaag
      301 caacaacata actcacccct gcatctgcag ctttcttctc taatgtaaga aatagtggct
      361 tccagtcacc tgtatcctta atttttagga tatcagatac cacatcatat ccaaaagaaa
      421 catgccccgt aatcttcaag tacttggcaa actgatctaa atgtttagcc cagtcatcat
      481 acttcaccga tgccaacgca ttaaaaatag catcacgatc ggctttgctg aatttcttat
      541 ttaaaacatc cttgtatttt tcaaaagcag cgagagcttc attcacattg ccgattttct
      601 tacctttaga cttatcagca agttcctgtg ccattttcga atatttttca ccatattttt
      661 cagtcagcgt ttgataaaag ctaactgttg catcaacagc atccttaatc tgtgaattaa
      721 ggagattatt ctgtgctttt ttcaaatttt cttcagcttc atgaacacga gcgataccgg
      781 cattacgatt attactgacc tgagaaatag ccttctggat ctgagttata tcagcattta
      841 tccggttaat acgtgtttct gatgctgtta cctgtttttg tttttcttct ctaatcttac
      901 cggccccaac ccgtcgtctg gttgcttcaa aaaaaggacg gttctgaagc ggatcattgg
      961 ctcttggtga tagttttttg accagctcat ccagttcttt atatttagcg gatgcctgag
     1021 ccagttcatt tcgttttcca gcgagcgttt tcatttctgc atcacgggca tggatactgg
     1081 agcttaaacg agaattgaga gtcttaatct ctccatccat tttcaccact tcagattgtg
     1141 cagcagaaag ttttttttgg gcgatctcaa cagctttagc ttcttcactc aatgcagcca
     1201 gtcgtttctc ttcagcttca gccagtttca actggcgttc tgtttcagcc ttctcccgtt
     1261 caatctcttt acgtcgttgt tctgcttcct gaaaagcctt ttctgctgct tccgcttctt
     1321 tacgggcttt ttcttctgct ttcgcaaggc gcaaacgctc tgcttccgcc tgcatagctg
     1381 cattattagc atgagcaagc tctgttgctg aaggcgtacg tgaggcattg tgacgaagag
     1441 cctcattcac gatatccttc aggcgctgag tcagcgcatc cctgtttgcc tttgctttcg
     1501 cctgtgcttc cgctgcagct tttgcccggg cagcctgctc tgcctgtgtt ttctttaatt
     1561 gagcagtaga ccatttagca gttgcatgaa tagctgcaga actttcactt ttactgcctc
     1621 cttttccacc tccgccgcca gagccactcc cgtcaggagt accattcaaa agagtaataa
     1681 ttacctgtcc cttatcatca taaggaacac catctttata gtacgctacc gcggtttcca
     1741 ttataaaatc ctctttgact tttaaaacaa taagttaaaa ataaatactg tacatataac
     1801 cactggtttt atatacagca taaaagctac gccgctgcat tttccctgtc aagactgtgg
     1861 acttccattt ttgtgaaaac gatcaaaaaa acagtctttc acaccacgcg ctattctcgc
     1921 ccgatgccac aaaaaccagc acaaacatta ccgttctcag acctcattat gttttactga
     1981 aactatgaga tgagacatct atgggacact gtcactttat ggcatggcac acactccggg
     2041 acgcactaaa aatgacaggc agatcgcgtt cacagtttta ccgtgatatg cgcgcaggcc
     2101 ttgtcagtta ccgtaccggc agggacggac gacgggagtt tgaaaccagt gaactgatcc
     2161 gggcatacgg cgaattaaag cagaatgaga caccagaaag gcacagtgag ggacatgcag
     2221 aaaatccaca tgatcagcag acagaacgca ttctccggga gctgaatgag ctgaaacaat
     2281 gcctgacgct gatgcttgag gataaacagg cacaggatat ggatcgcaga cgccaggaag
     2341 cagaacggga acagctacaa aatgagatag cccagctcag gcaggcactg gaactggaaa
     2401 agaaacgggg attctggtcc aggttgttcg gtcgctgaac gctgtcagag actgatgata
     2461 aaatagtctt cggataataa ctcaccgaga ataaatactt taaggtaggg agacactcat
     2521 gagacgtacc ggaaacaaac tttgtcttat cgccatgata acagcaacag tagctctcac
     2581 agcctgtacc ccaaagggca gcgtggaaca acatacccgg cattacgtat atgcttctga
     2641 tgacggtttt gatcccaact tttccaccca aaaagccgac acaacacgaa tgatggtgcc
     2701 tttttttcgg cagttctggg atatgggagc taaagataaa gcgacaggaa aatcacggag
     2761 tgatgtgcaa caacgcattc agcagtttca cagccaggaa tttttaaact cactccgggg
     2821 cacaactcaa tttgcgggta ctgattaccg cagcaaagac cttaccccga aaaaatccag
     2881 gctgctggct gacacgattt ctgcggttta tctcgatggc tacgagggca gacagtaagt
     2941 ggatttacca taatccctta attgtacgca ccgctaaaac gcgttcagcg cgatcacggc
     3001 agcagacagg taaaaatggc aacaaaccac ccgaaaaact gccgcgatcg cgcctgataa
     3061 attttaaccg tatgaatacc tatgcaacca gagggtacag gccacattac ccccacttaa
     3121 tccactgaag ctgccatttt tcatggtttc accatcccag cgaagggcca tccagcgtgc
     3181 gttcctgtat ttccggctga cgctcccgtt ccagggataa cacatgttcg cgctcctgta
     3241 tcagccgttc ctctcttatc tccagttctc gctgtataac tggctcaagc gttctgtctg
     3301 ctcgctcaag tgctgcacct gctgactcaa ctgcatgacc cgctcgttca gcatcgcgtt
     3361 gtcccgttgc gtaagcgaaa acatcttctg caattccacg aaggcgctct cccattcgct
     3421 cagccgctgc atatagtcct gttgcagctg ctctaaggcg ttcagcaaat gtgtttccag
     3481 ctctgtcact ctgtgtcact ccttcagatg tacccactct ttcccctgaa agggaatcac
     3541 ctccgctgat ttcccgtacg gaaggacaag gaatttcctg ttcccgtcct gcacaaactc
     3601 cacgccccat gtcttcgcgt tcagtttctg caatgtctct tcctgcttcc tgatttcttc
     3661 caggttcgcc tgtatcctcc ctccaagata ccagagcgtc ccgccactcg cggtaaacag
     3721 gagaaagact atccccagta acatcatgcc cgtattccct gccagcttta acacgtccct
     3781 cctgtgctgc atcatcgcct ctttcacccc ttcccggtgt ttttccagcg attcctctgt
     3841 cgaggctgtg aacagggcta tagcgtctct gattttcgtc tcgtttgatg tcacagcctc
     3901 gcttacagat tcgccgagcc tcctgaactc gttgttcagc attttctctg tagattcggc
     3961 tctctctttc agctttttct cgaactccgc gcccgtctgc aaaagattgc tcataaaatg
     4021 ctcctttcag cctgatattc ttcccgccgt tcggatctgc aatgctgata ctgcttcgcg
     4081 tcaccctgac cacttccagc cccgcctcag tgagcgcctg aatcacatcc tgacggcctt
     4141 ttatctctcc ggcatggtaa agtgcatcta tacctcgcgt gacgccctca gcaagcgcct
     4201 gtttcgtttc aggcaggtta tcagggagtg tcagcgtcct gcggttctcc ggggcgttcg
     4261 ggtcatgcag cccgtaatgg tgattcacca gcgtctgcca ggcatcaatt ctaggcctgt
     4321 ctgcgcggtc gtagtacggc tggaggcgtt ttccggtctg tagctccatg ttcggaatga
     4381 caaaattcag ctcaagccgt cccttgtcct ggtgctccac ccacaggatg ctgtactgat
     4441 ttttttcgag accgggcatc agtacacgct caaagctcgc catcactttt tcacgtcctc
     4501 ccggcggcag ctccttctcc gcgaacgaca gaacaccgga cgtgtatttc ttcgcaaatg
     4561 gcgtggcatc gatgagttcc cggacttctt ccggattacc ctgaagcacc gttgcgcctt
     4621 cgcggttacg ctccctcccc agcaggtaat caaccggacc actgccacca ccttttcccc
     4681 tggcatgaaa tttaactatc atcccgcgcc ccctgttccc tgacggccag acgcagccgg
     4741 cgcagctcat ccccgatggc catcagtgcg gccaccacct gaacccggtc accggaagac
     4801 cactgcccgc tgttcacctt acgggctgtc tgattcaggt tatttccgat ggcggccagc
     4861 tgacgcagta acggcggtgc cagtgtcggc agttttccgg aacgggcaac cggctccccc
     4921 aggcagaccc gccgcatcca taccgccagt tgtttaccct cacagcgttc aagtaaccgg
     4981 gcatgttcat catcagtaac ccgtattgtg agcatcctct cgcgtttcat cggtatcatt
     5041 accccatgaa cagaaatccc ccttacacgg aggcatcagt gactaaacag gaaaaaaccg
     5101 cccttaacat ggcccgcttt atcagaagcc agacattaac gctgctggag aagctcaacg
     5161 aactggacgc agatgaacag gccgatattt gtgaatcgct tcacgaccac gccgatgagc
     5221 tttaccgcag ctgcctcgca cgtttcgggg atgacggtga aaacctctga tacatgcagc
     5281 tcccggagac ggtcacagct tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg
     5341 gcgcgtcagc aggttttagc gggtgtcggg gcgcagccct gacccagtca cgtagcgata
     5401 gcggagtgta tactggctta accatgcggc atcagtgcgg attgtatgaa aagtacgcca
     5461 tgccgggtgt gaaatgccgc acagatgcgt aaggagaaaa tgcacgtcca ggcgcttttc
     5521 cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag cggtactgac
     5581 tcacacaaaa acggtaacac agttatccac agaatcaggg gataaggccg gaaagaacat
     5641 gtgagcaaaa gaccaggaac aggaagaagg ccacgtagca ggcgtttttc cataggctcc
     5701 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     5761 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     5821 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     5881 atagctcacg ctgttggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     5941 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6001 ccaacccggt aaggcacgcc ttaacgccac tggcagcagc cactggtaac cggattagca
     6061 gagcgatgat ggcacaaacg gtgctacaga gttcttgaag tagtggcccg actacggcta
     6121 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6181 agttggtagc tcttgatccg gcaaacaaac caccgttggt agcggtggtt tttttgtttg
     6241 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6301 tgaaccgcga tccccgtcag tttagaagag gaggatggtg cgatggtccc tccctgaaca
     6361 tcacgtatat agttagcctg acatccaaca aggaggttta tcgcgaatat tcccacaaaa
     6421 aatcttttcc tcataactcg atccttataa aatgaaaaga atatatggcg aggtttaatt
     6481 tatgagctta agatactaca taaaaaatat tttatttggc ctgtactgca cacttatata
     6541 tatatacctt ataacaaaaa acagcgaagg gtattatttc cttgtgtcag ataagatgct
     6601 atatgcaata gtgataagca ctattctatg tccatattca aaatatg
//
