LOCUS       NZ_CP086487             6647 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Escherichia coli strain Ec-050-T20-MAC plasmid pEc-050-T20-MAC_7,
            complete sequence.
ACCESSION   NZ_CP086487
VERSION     NZ_CP086487.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22108724
            Assembly: GCF_022759765.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Genome plasticity in E. coli and K. pneumoniae: SNV accumulation
            over time
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 308x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022759765.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 04:40:39
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,280
            CDSs (total)                      :: 5,158
            Genes (coding)                    :: 4,886
            CDSs (with protein)               :: 4,886
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 272
            CDSs (without protein)            :: 272
            Pseudo Genes (ambiguous residues) :: 0 of 272
            Pseudo Genes (frameshifted)       :: 108 of 272
            Pseudo Genes (incomplete)         :: 176 of 272
            Pseudo Genes (internal stop)      :: 53 of 272
            Pseudo Genes (multiple problems)  :: 57 of 272
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086487.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec-050-T20-MAC"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc-050-T20-MAC_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            249..767
                     /locus_tag="LNN46_RS26135"
                     /old_locus_tag="LNN46_26140"
     CDS             249..767
                     /locus_tag="LNN46_RS26135"
                     /old_locus_tag="LNN46_26140"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            774..1007
                     /locus_tag="LNN46_RS26140"
                     /old_locus_tag="LNN46_26145"
     CDS             774..1007
                     /locus_tag="LNN46_RS26140"
                     /old_locus_tag="LNN46_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1325..1744)
                     /locus_tag="LNN46_RS26145"
                     /old_locus_tag="LNN46_26150"
     CDS             complement(1325..1744)
                     /locus_tag="LNN46_RS26145"
                     /old_locus_tag="LNN46_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(1825..2262)
                     /locus_tag="LNN46_RS26150"
                     /old_locus_tag="LNN46_26155"
     CDS             complement(1825..2262)
                     /locus_tag="LNN46_RS26150"
                     /old_locus_tag="LNN46_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2522..4090
                     /gene="cea"
                     /locus_tag="LNN46_RS26155"
                     /old_locus_tag="LNN46_26160"
     CDS             2522..4090
                     /gene="cea"
                     /locus_tag="LNN46_RS26155"
                     /old_locus_tag="LNN46_26160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(4087..4428)
                     /locus_tag="LNN46_RS26160"
                     /old_locus_tag="LNN46_26165"
     CDS             complement(4087..4428)
                     /locus_tag="LNN46_RS26160"
                     /old_locus_tag="LNN46_26165"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            4476..4613
                     /locus_tag="LNN46_RS26165"
                     /old_locus_tag="LNN46_26170"
     CDS             4476..4613
                     /locus_tag="LNN46_RS26165"
                     /old_locus_tag="LNN46_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(4812..4970)
                     /locus_tag="LNN46_RS26490"
     CDS             complement(4812..4970)
                     /locus_tag="LNN46_RS26490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(5640..5831)
                     /locus_tag="LNN46_RS26170"
                     /old_locus_tag="LNN46_26175"
     CDS             complement(5640..5831)
                     /locus_tag="LNN46_RS26170"
                     /old_locus_tag="LNN46_26175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5880..6218
                     /locus_tag="LNN46_RS26175"
                     /old_locus_tag="LNN46_26180"
     CDS             5880..6218
                     /locus_tag="LNN46_RS26175"
                     /old_locus_tag="LNN46_26180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_001405931.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVR
                     EQGARDDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          5468..5555
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(4899..5454)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          3..1111
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.143"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          6209..6645
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.8e-90"
ORIGIN
        1 cccgaacgcc ccggagaacc gcaggacgct gacactccct gataacctgc ctgaaacgaa
       61 acaggcgctt gctgagggcg tcacgcgagg tatagatgca ctttaccatg ccggagagat
      121 aaaaggccgt caggatgtga ttcaggcgct cactgaggcg gggctggaag tggtcagggt
      181 gacgcgaagc agtatcagca ttgcagatcc gaacggcggg aagaatatca ggctgaaagg
      241 agcattttat gagcaatctt ttgcagacgg gcgcggagtt cgagaaaaag ctgaaagaga
      301 gagccgaatc tacagagaaa atgctgaaca acgagttcag gaggctcggc gaatctgtaa
      361 gcgaggctgt gacatcaaac gagacgaaaa tcagagacgc tatagccctg ttcacagcct
      421 cgacagagga atcgctggaa aaacaccggg aaggggtgaa agaggcgatg atgcagcaca
      481 ggagggacgt gttaaagctg gcagggaata cgggcatgat gttactgggg atagtctttc
      541 tcctgtttac cgcgagtggc gggacgctct ggtatcttgg agggaggata caggcgaacc
      601 tggaagaaat caggaagcag gaagagacat tgcagaaact gaacgcgaag acatggggcg
      661 tggagtttgt gcaggacggg aacaggaaat tccttgtcct tccgtacggg aaatcagcgg
      721 aggtgattcc ctttcagggg aaagagtggg tacatctgaa ggagtgacac agagtgacag
      781 agctggaaac acatttgctg aacgccttag agcagctgca acaggactat atgcagcggc
      841 tgagcgaatg ggagagcgcc ttcgtggaat tgcagaagat gttttcgctt acgcaacggg
      901 acaacgcgat gctgaacgag cgggtcatgc agttgagtca gcaggtgcag cacttgagcg
      961 agcagacaga acgcttgagc cagttataca gcgagaactg gagataagag aggaacggct
     1021 gatacaggag cgcgaacatg tgttatccct ggaacgggag cgtcagccgg aaatacagga
     1081 acgcacgctg gatggccctt cgctgggatg gtgaaaccat gaaaaatggc agcttcagtg
     1141 gattaagtgg gggtaatgtg gcctgtaccc tctggttgca taggtattca tacggttaaa
     1201 atttatcagg cgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg
     1261 ctgccgtgat cgcgctgaac gcgttttagc ggtgcgtaca attaagggat tatggtaaat
     1321 ccacttactg tctgccctcg tagccatcga gataaaccgc agaaatcgtg tcagccagca
     1381 gcctggattt tttcggggta aggtctttgc tgcggtaatc agtacccgca aattgagttg
     1441 tgccccggag tgagtttaaa aattcctggc tgtgaaactg ctgaatgcgt tgttgcacat
     1501 cactccgtga ttttcctgtc gctttatctt tagctcccat atcccagaac tgccgaaaaa
     1561 aaggcaccat cattcgtgtt gtgtcggctt tttgggtgga aaagttggga tcaaaaccgt
     1621 catcagaagc atatacgtaa tgccgggtat gttgttccac gctgcccttt ggggtacagg
     1681 ctgtgagagc tactgttgct gttatcatgg cgataagaca aagtttgttt ccggtacgtc
     1741 tcatgagtgt ctccctacct taaagtattt attctcggtg agttattatc cgaagactat
     1801 tttatcatca gtctctgaca gcgttcagcg accgaacaac ctggaccaga atccccgttt
     1861 cttttccagt tccagtgcct gcctgagctg ggctatctca ttttgtagct gttcccgttc
     1921 tgcttcctgg cgtctgcgat ccatatcctg tgcctgttta tcctcaagca tcagcgtcag
     1981 gcattgtttc agctcattca gctcccggag aatgcgttct gtctgctgat catgtggatt
     2041 ttctgcatgt ccctcactgt gcctttctgg tgtctcattc tgctttaatt cgccgtatgc
     2101 ccggatcagt tcactggttt caaactcccg tcgtccgtcc ctgccggtac ggtaactgac
     2161 aaggcctgcg cgcatatcac ggtaaaactg tgaacgcgat ctgcctgtca tttttagtgc
     2221 gtcccggagt gtgtgccatg ccataaagtg acagtgtccc atagatgtct catctcatag
     2281 tttcagtaaa acataatgag gtctgagaac ggtaatgttt gtgctggttt ttgtggcatc
     2341 gggcgagaat agcgcgtggt gtgaaagact gtttttttga tcgttttcac aaaaatggaa
     2401 gtccacagtc ttgacaggga aaatgcagcg gcgtagcttt tatgctgtat ataaaaccag
     2461 tggttatatg tacagtattt atttttaact tattgtttta aaagtcaaag aggattttat
     2521 aatggaaacc gcggtagcgt actataaaga tggtgttcct tatgatgata agggacaggt
     2581 aattattact cttttgaatg gtactcctga cgggagtggc tctggcggcg gaggtggaaa
     2641 aggaggcagt aaaagtgaaa gttctgcagc tattcatgca actgctaaat ggtctactgc
     2701 tcaattaaag aaaacacagg cagagcaggc tgcccgggca aaagctgcag cggaagcaca
     2761 ggcgaaagca aaggcaaaca gggatgcgct gactcagcgc ctgaaggata tcgtgaatga
     2821 ggctcttcgt cacaatgcct cacgtacgcc ttcagcaaca gagcttgctc atgctaataa
     2881 tgcagctatg caggcggaag cagagcgttt gcgccttgcg aaagcagaag aaaaagcccg
     2941 taaagaagcg gaagcagcag aaaaggcttt tcaggaagca gaacaacgac gtaaagagat
     3001 tgaacgggag aaggctgaaa cagaacgcca gttgaaactg gctgaagctg aagagaaacg
     3061 actggctgca ttgagtgaag aagctaaagc tgttgagatc gcccaaaaaa aactttctgc
     3121 tgcacaatct gaagtggtga aaatggatgg agagattaag actctcaatt ctcgtttaag
     3181 ctccagtatc catgcccgtg atgcagaaat gaaaacgctc gctggaaaac gaaatgaact
     3241 ggctcaggca tccgctaaat ataaagaact ggatgagctg gtcaaaaaac tatcaccaag
     3301 agccaatgat ccgcttcaga accgtccttt ttttgaagca accagacgac gggttggggc
     3361 cggtaagatt agagaagaaa aacaaaaaca ggtaacagca tcagaaacac gtattaaccg
     3421 gataaatgct gatataactc agatccagaa ggctatttct caggtcagta ataatcgtaa
     3481 tgccggtatc gctcgtgttc atgaagctga agaaaatttg aaaaaagcac agaataatct
     3541 ccttaattca cagattaagg atgctgttga tgcaacagtt agcttttatc aaacgctgac
     3601 tgaaaaatat ggtgaaaaat attcgaaaat ggcacaggaa cttgctgata agtctaaagg
     3661 taagaaaatc ggcaatgtga atgaagctct cgctgctttt gaaaaataca aggatgtttt
     3721 aaataagaaa ttcagcaaag ccgatcgtga tgctattttt aatgcgttgg catcggtgaa
     3781 gtatgatgac tgggctaaac atttagatca gtttgccaag tacttgaaga ttacggggca
     3841 tgtttctttt ggatatgatg tggtatctga tatcctaaaa attaaggata caggtgactg
     3901 gaagccacta tttcttacat tagagaagaa agctgcagat gcaggggtga gttatgttgt
     3961 tgctttactt tttagcttgc ttgctggaac tacattaggt atttggggta ttgctattgt
     4021 tacaggaatt ctatgctcct atattgataa gaataaactt aatactataa atgaggtgtt
     4081 agggatttaa ttattcttta ttgatataaa aagtcctagc aatccaaatg ggattgctag
     4141 gaccaaacaa agtagattat atagcataaa taggtttaat tttgctacgg gggcgttatt
     4201 taggtttttt cttctttcga aaaaatcttt ctttatgaag ttaaaagcta tgtgttcaat
     4261 agcatatttt gaatatggac atagaatagt gcttatcact attgcatata gcatcttatc
     4321 tgacacaagg aaataatacc cttcgctgtt ttttgttata aggtatatat atataagtgt
     4381 gcagtacagg ccaaataaaa tattttttat gtagtatctt aagctcataa attaaacctc
     4441 gccatatatt cttttcattt tataaggatc gagttatgag gaaaagattt tttgtgggaa
     4501 tattcgcgat aaacctcctt gttggatgtc aggctaacta tatacgtgat gttcagggag
     4561 ggaccatcgc accatcctcc tcttctaaac tgacggggat cgcggttcag tagaaaagat
     4621 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     4681 accaccgcta ccaacggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     4741 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtc
     4801 gggccactac ttcaagaact ctgtagcacc gtttgtgcca tcatcgctct gctaatccgg
     4861 ttaccagtgg ctgctgccag tggcgttaag gcgtgcctta ccgggttgga ctcaagacga
     4921 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     4981 ttggagcgaa cgacctacac cgaactgaga taccaacagc gtgagctatg agaaagcgcc
     5041 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     5101 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     5161 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     5221 aaaaacgcct gctacgtggc cttcttcctg ttcctggtct tttgctcaca tgttctttcc
     5281 ggccttatcc cctgattctg tggataactg tgttaccgtt tttgtgtgag tcagtaccgc
     5341 tcgccgcagt cgaacgaccg agcgtagcga gtcagtgagc gaggaagcgg aaaagcgcct
     5401 ggacgtgcat tttctcctta cgcatctgtg cggcatttca cacccggcat ggcgtacttt
     5461 tcatacaatc cgcactgatg ccgcatggtt aagccagtat acactccgct atcgctacgt
     5521 gactgggtca gggctgcgcc ccgacacccg ctaaaacctg ctgacgcgcc ctgacgggct
     5581 tgtcagctcc cggcatccgc tcacagacaa gctgtgaccg tctccgggag ctgcatgtat
     5641 cagaggtttt caccgtcatc cccgaaacgt gcgaggcagc tgcggtaaag ctcatcggcg
     5701 tggtcgtgaa gcgattcaca aatatcggcc tgttcatctg cgtccagttc gttgagcttc
     5761 tccagcagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
     5821 tgtttagtca ctgatgcctc cgtgtaaggg ggatttctgt tcatggggta atgataccga
     5881 tgaaacgcga gaggatgctc acaatacggg ttactgatga tgaacatgcc cggttacttg
     5941 aacgctgtga gggtaaacaa ctggcggtat ggatgcggcg ggtctgcctg ggggagccgg
     6001 ttgcccgttc cggaaaactg ccgacactgg caccgccgtt actgcgtcag ctggccgcca
     6061 tcggaaataa cctgaatcag acagcccgta aggtgaacag cgggcagtgg tcttccggtg
     6121 accgggttca ggtggtggcc gcactgatgg ccatcgggga tgagctgcgc cggctgcgtc
     6181 tggccgtcag ggaacagggg gcgcgggatg atagttaaat ttcatgccag gggaaaaggt
     6241 ggtggcagtg gtccggttga ttacctgctg gggagggagc gtaaccgcga aggcgcaacg
     6301 gtgcttcagg gtaatccgga agaagtccgg gaactcatcg atgccacgcc atttgcgaag
     6361 aaatacacgt ccggtgttct gtcgttcgcg gagaaggagc tgccgccggg aggacgtgaa
     6421 aaagtgatgg cgagctttga gcgtgtactg atgcccggtc tcgaaaaaaa tcagtacagc
     6481 atcctgtggg tggagcacca ggacaaggga cggcttgagc tgaattttgt cattccgaac
     6541 atggagctac agaccggaaa acgcctccag ccgtactacg accgcgcaga caggcctaga
     6601 attgatgcct ggcagacgct ggtgaatcac cattacgggc tgcatga
//
