LOCUS       NZ_CP086477             6647 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Escherichia coli strain Ec-050-T20-ESBL plasmid pEc-050-T20-ESBL_7,
            complete sequence.
ACCESSION   NZ_CP086477
VERSION     NZ_CP086477.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22108725
            Assembly: GCF_022759785.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Genome plasticity in E. coli and K. pneumoniae: SNV accumulation
            over time
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6647)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 390x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022759785.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 04:58:40
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,279
            CDSs (total)                      :: 5,157
            Genes (coding)                    :: 4,884
            CDSs (with protein)               :: 4,884
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 273
            CDSs (without protein)            :: 273
            Pseudo Genes (ambiguous residues) :: 0 of 273
            Pseudo Genes (frameshifted)       :: 109 of 273
            Pseudo Genes (incomplete)         :: 176 of 273
            Pseudo Genes (internal stop)      :: 53 of 273
            Pseudo Genes (multiple problems)  :: 57 of 273
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086477.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="Ec-050-T20-ESBL"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEc-050-T20-ESBL_7"
                     /geo_loc_name="Switzerland"
                     /collection_date="2018"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            complement(join(6515..6647,1..287))
                     /locus_tag="LNN47_RS26120"
                     /old_locus_tag="LNN47_26125"
     CDS             complement(join(6515..6647,1..287))
                     /locus_tag="LNN47_RS26120"
                     /old_locus_tag="LNN47_26125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="WP_001254967.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="Exc2 family lipoprotein"
     gene            complement(368..805)
                     /locus_tag="LNN47_RS26125"
                     /old_locus_tag="LNN47_26130"
     CDS             complement(368..805)
                     /locus_tag="LNN47_RS26125"
                     /old_locus_tag="LNN47_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001405929.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            1065..2633
                     /gene="cea"
                     /locus_tag="LNN47_RS26130"
                     /old_locus_tag="LNN47_26135"
     CDS             1065..2633
                     /gene="cea"
                     /locus_tag="LNN47_RS26130"
                     /old_locus_tag="LNN47_26135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin E1"
                     /protein_id="WP_000447006.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="cea"
     gene            complement(2630..2971)
                     /locus_tag="LNN47_RS26135"
                     /old_locus_tag="LNN47_26140"
     CDS             complement(2630..2971)
                     /locus_tag="LNN47_RS26135"
                     /old_locus_tag="LNN47_26140"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 immunity protein"
                     /protein_id="WP_000058759.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="colicin E1 immunity protein"
     gene            3019..3156
                     /locus_tag="LNN47_RS26140"
                     /old_locus_tag="LNN47_26145"
     CDS             3019..3156
                     /locus_tag="LNN47_RS26140"
                     /old_locus_tag="LNN47_26145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_001405930.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTIAPSSSSKLTGIAVQ
                     "
                     /name="colicin release lysis protein"
     gene            complement(3355..3513)
                     /locus_tag="LNN47_RS26485"
     CDS             complement(3355..3513)
                     /locus_tag="LNN47_RS26485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071531517.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHALTPLAAATGNRISRAMMA
                     QTVLQSS"
                     /name="replication initiation protein"
     gene            complement(4183..4374)
                     /locus_tag="LNN47_RS26145"
                     /old_locus_tag="LNN47_26150"
     CDS             complement(4183..4374)
                     /locus_tag="LNN47_RS26145"
                     /old_locus_tag="LNN47_26150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165985.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4438..4761
                     /locus_tag="LNN47_RS26150"
                     /old_locus_tag="LNN47_26155"
     CDS             4438..4761
                     /locus_tag="LNN47_RS26150"
                     /old_locus_tag="LNN47_26155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955999.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            4970..>5401
                     /locus_tag="LNN47_RS26155"
                     /pseudo=""
     CDS             4970..>5401
                     /locus_tag="LNN47_RS26155"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            5439..5957
                     /locus_tag="LNN47_RS26160"
                     /old_locus_tag="LNN47_26165"
     CDS             5439..5957
                     /locus_tag="LNN47_RS26160"
                     /old_locus_tag="LNN47_26165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067500.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            5964..6197
                     /locus_tag="LNN47_RS26165"
                     /old_locus_tag="LNN47_26170"
     CDS             5964..6197
                     /locus_tag="LNN47_RS26165"
                     /old_locus_tag="LNN47_26170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          4011..4098
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(3442..3997)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.878"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4752..6301
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.473"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaattcct ggctgtgaaa ctgctgaatg cgttgttgca catcactccg tgattttcct
       61 gtcgctttat ctttagctcc catatcccag aactgccgaa aaaaaggcac catcattcgt
      121 gttgtgtcgg ctttttgggt ggaaaagttg ggatcaaaac cgtcatcaga agcatatacg
      181 taatgccggg tatgttgttc cacgctgccc tttggggtac aggctgtgag agctactgtt
      241 gctgttatca tggcgataag acaaagtttg tttccggtac gtctcatgag tgtctcccta
      301 ccttaaagta tttattctcg gtgagttatt atccgaagac tattttatca tcagtctctg
      361 acagcgttca gcgaccgaac aacctggacc agaatccccg tttcttttcc agttccagtg
      421 cctgcctgag ctgggctatc tcattttgta gctgttcccg ttctgcttcc tggcgtctgc
      481 gatccatatc ctgtgcctgt ttatcctcaa gcatcagcgt caggcattgt ttcagctcat
      541 tcagctcccg gagaatgcgt tctgtctgct gatcatgtgg attttctgca tgtccctcac
      601 tgtgcctttc tggtgtctca ttctgcttta attcgccgta tgcccggatc agttcactgg
      661 tttcaaactc ccgtcgtccg tccctgccgg tacggtaact gacaaggcct gcgcgcatat
      721 cacggtaaaa ctgtgaacgc gatctgcctg tcatttttag tgcgtcccgg agtgtgtgcc
      781 atgccataaa gtgacagtgt cccatagatg tctcatctca tagtttcagt aaaacataat
      841 gaggtctgag aacggtaatg tttgtgctgg tttttgtggc atcgggcgag aatagcgcgt
      901 ggtgtgaaag actgtttttt tgatcgtttt cacaaaaatg gaagtccaca gtcttgacag
      961 ggaaaatgca gcggcgtagc ttttatgctg tatataaaac cagtggttat atgtacagta
     1021 tttattttta acttattgtt ttaaaagtca aagaggattt tataatggaa accgcggtag
     1081 cgtactataa agatggtgtt ccttatgatg ataagggaca ggtaattatt actcttttga
     1141 atggtactcc tgacgggagt ggctctggcg gcggaggtgg aaaaggaggc agtaaaagtg
     1201 aaagttctgc agctattcat gcaactgcta aatggtctac tgctcaatta aagaaaacac
     1261 aggcagagca ggctgcccgg gcaaaagctg cagcggaagc acaggcgaaa gcaaaggcaa
     1321 acagggatgc gctgactcag cgcctgaagg atatcgtgaa tgaggctctt cgtcacaatg
     1381 cctcacgtac gccttcagca acagagcttg ctcatgctaa taatgcagct atgcaggcgg
     1441 aagcagagcg tttgcgcctt gcgaaagcag aagaaaaagc ccgtaaagaa gcggaagcag
     1501 cagaaaaggc ttttcaggaa gcagaacaac gacgtaaaga gattgaacgg gagaaggctg
     1561 aaacagaacg ccagttgaaa ctggctgaag ctgaagagaa acgactggct gcattgagtg
     1621 aagaagctaa agctgttgag atcgcccaaa aaaaactttc tgctgcacaa tctgaagtgg
     1681 tgaaaatgga tggagagatt aagactctca attctcgttt aagctccagt atccatgccc
     1741 gtgatgcaga aatgaaaacg ctcgctggaa aacgaaatga actggctcag gcatccgcta
     1801 aatataaaga actggatgag ctggtcaaaa aactatcacc aagagccaat gatccgcttc
     1861 agaaccgtcc tttttttgaa gcaaccagac gacgggttgg ggccggtaag attagagaag
     1921 aaaaacaaaa acaggtaaca gcatcagaaa cacgtattaa ccggataaat gctgatataa
     1981 ctcagatcca gaaggctatt tctcaggtca gtaataatcg taatgccggt atcgctcgtg
     2041 ttcatgaagc tgaagaaaat ttgaaaaaag cacagaataa tctccttaat tcacagatta
     2101 aggatgctgt tgatgcaaca gttagctttt atcaaacgct gactgaaaaa tatggtgaaa
     2161 aatattcgaa aatggcacag gaacttgctg ataagtctaa aggtaagaaa atcggcaatg
     2221 tgaatgaagc tctcgctgct tttgaaaaat acaaggatgt tttaaataag aaattcagca
     2281 aagccgatcg tgatgctatt tttaatgcgt tggcatcggt gaagtatgat gactgggcta
     2341 aacatttaga tcagtttgcc aagtacttga agattacggg gcatgtttct tttggatatg
     2401 atgtggtatc tgatatccta aaaattaagg atacaggtga ctggaagcca ctatttctta
     2461 cattagagaa gaaagctgca gatgcagggg tgagttatgt tgttgcttta ctttttagct
     2521 tgcttgctgg aactacatta ggtatttggg gtattgctat tgttacagga attctatgct
     2581 cctatattga taagaataaa cttaatacta taaatgaggt gttagggatt taattattct
     2641 ttattgatat aaaaagtcct agcaatccaa atgggattgc taggaccaaa caaagtagat
     2701 tatatagcat aaataggttt aattttgcta cgggggcgtt atttaggttt tttcttcttt
     2761 cgaaaaaatc tttctttatg aagttaaaag ctatgtgttc aatagcatat tttgaatatg
     2821 gacatagaat agtgcttatc actattgcat atagcatctt atctgacaca aggaaataat
     2881 acccttcgct gttttttgtt ataaggtata tatatataag tgtgcagtac aggccaaata
     2941 aaatattttt tatgtagtat cttaagctca taaattaaac ctcgccatat attcttttca
     3001 ttttataagg atcgagttat gaggaaaaga ttttttgtgg gaatattcgc gataaacctc
     3061 cttgttggat gtcaggctaa ctatatacgt gatgttcagg gagggaccat cgcaccatcc
     3121 tcctcttcta aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag
     3181 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccaacgg
     3241 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     3301 gagcgcagat accaaatact gtccttctag tgtagccgta gtcgggccac tacttcaaga
     3361 actctgtagc accgtttgtg ccatcatcgc tctgctaatc cggttaccag tggctgctgc
     3421 cagtggcgtt aaggcgtgcc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3481 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3541 caccgaactg agataccaac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     3601 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3661 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     3721 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg cctgctacgt
     3781 ggccttcttc ctgttcctgg tcttttgctc acatgttctt tccggcctta tcccctgatt
     3841 ctgtggataa ctgtgttacc gtttttgtgt gagtcagtac cgctcgccgc agtcgaacga
     3901 ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg cattttctcc
     3961 ttacgcatct gtgcggcatt tcacacccgg catggcgtac ttttcataca atccgcactg
     4021 atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg tcagggctgc
     4081 gccccgacac ccgctaaaac ctgctgacgc gccctgacgg gcttgtcagc tcccggcatc
     4141 cgctcacaga caagctgtga ccgtctccgg gagctgcatg tatcagaggt tttcaccgtc
     4201 atccccgaaa cgtgcgaggc agctgcggta aagctcatcg gcgtggtcgt gaagcgattc
     4261 acaaatatcg gcctgttcat ctgcgtccag ttcgttgagc ttctccagca gcgttaatgt
     4321 ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttag tcactgatgc
     4381 ctccgtgtaa gggggatttc tgttcatggg gtaatgatac cgatgaaacg cgagaggatg
     4441 ctcacaatac gggttactga tgatgaacat gcccggttac ttgaacgctg tgagggtaaa
     4501 caactggcgg tatggatgcg gcgggtctgc ctgggggagc cggttgcccg ttccggaaaa
     4561 ctgccgacac tggcaccgcc gttactgcgt cagctggccg ccatcggaaa taacctgaat
     4621 cagacagccc gtaaggtgaa cagcgggcag tggtcttccg gtgaccgggt tcaggtggtg
     4681 gccgcactga tggccatcgg ggatgagctg cgccggctgc gtctggccgt cagggaacag
     4741 ggggcgcggg atgatagtta aatttcatgc caggggaaaa ggtggtggca gtggtccggt
     4801 tgattacctg ctggggaggg agcgtaaccg cgaaggcgca acggtgcttc agggtaatcc
     4861 ggaagaagtc cgggaactca tcgatgccac gccatttgcg aagaaataca cgtccggtgt
     4921 tctgtcgttc gcggagaagg agctgccgcc gggaggacgt gaaaaagtga tggcgagctt
     4981 tgagcgtgta ctgatgcccg gtctcgaaaa aaatcagtac agcatcctgt gggtggagca
     5041 ccaggacaag ggacggcttg agctgaattt tgtcattccg aacatggagc tacagaccgg
     5101 aaaacgcctc cagccgtact acgaccgcgc agacaggcct agaattgatg cctggcagac
     5161 gctggtgaat caccattacg ggctgcatga cccgaacgcc ccggagaacc gcaggacgct
     5221 gacactccct gataacctgc ctgaaacgaa acaggcgctt gctgagggcg tcacgcgagg
     5281 tatagatgca ctttaccatg ccggagagat aaaaggccgt caggatgtga ttcaggcgct
     5341 cactgaggcg gggctggaag tggtcagggt gacgcgaagc agtatcagca ttgcagatcc
     5401 gaacggcggg aagaatatca ggctgaaagg agcattttat gagcaatctt ttgcagacgg
     5461 gcgcggagtt cgagaaaaag ctgaaagaga gagccgaatc tacagagaaa atgctgaaca
     5521 acgagttcag gaggctcggc gaatctgtaa gcgaggctgt gacatcaaac gagacgaaaa
     5581 tcagagacgc tatagccctg ttcacagcct cgacagagga atcgctggaa aaacaccggg
     5641 aaggggtgaa agaggcgatg atgcagcaca ggagggacgt gttaaagctg gcagggaata
     5701 cgggcatgat gttactgggg atagtctttc tcctgtttac cgcgagtggc gggacgctct
     5761 ggtatcttgg agggaggata caggcgaacc tggaagaaat caggaagcag gaagagacat
     5821 tgcagaaact gaacgcgaag acatggggcg tggagtttgt gcaggacggg aacaggaaat
     5881 tccttgtcct tccgtacggg aaatcagcgg aggtgattcc ctttcagggg aaagagtggg
     5941 tacatctgaa ggagtgacac agagtgacag agctggaaac acatttgctg aacgccttag
     6001 agcagctgca acaggactat atgcagcggc tgagcgaatg ggagagcgcc ttcgtggaat
     6061 tgcagaagat gttttcgctt acgcaacggg acaacgcgat gctgaacgag cgggtcatgc
     6121 agttgagtca gcaggtgcag cacttgagcg agcagacaga acgcttgagc cagttataca
     6181 gcgagaactg gagataagag aggaacggct gatacaggag cgcgaacatg tgttatccct
     6241 ggaacgggag cgtcagccgg aaatacagga acgcacgctg gatggccctt cgctgggatg
     6301 gtgaaaccat gaaaaatggc agcttcagtg gattaagtgg gggtaatgtg gcctgtaccc
     6361 tctggttgca taggtattca tacggttaaa atttatcagg cgcgatcgcg gcagtttttc
     6421 gggtggtttg ttgccatttt tacctgtctg ctgccgtgat cgcgctgaac gcgttttagc
     6481 ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga
     6541 gataaaccgc agaaatcgtg tcagccagca gcctggattt tttcggggta aggtctttgc
     6601 tgcggtaatc agtacccgca aattgagttg tgccccggag tgagttt
//
