LOCUS       NZ_CP086453             4052 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Klebsiella pneumoniae strain AE-2247421-T0 plasmid p2247421-T0_6,
            complete sequence.
ACCESSION   NZ_CP086453
VERSION     NZ_CP086453.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22108731
            Assembly: GCF_022760005.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Genome plasticity in E. coli and K. pneumoniae: SNV accumulation
            over time
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 223x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022760005.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 04:59:44
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,951
            CDSs (total)                      :: 5,830
            Genes (coding)                    :: 5,656
            CDSs (with protein)               :: 5,656
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 174
            CDSs (without protein)            :: 174
            Pseudo Genes (ambiguous residues) :: 0 of 174
            Pseudo Genes (frameshifted)       :: 66 of 174
            Pseudo Genes (incomplete)         :: 104 of 174
            Pseudo Genes (internal stop)      :: 28 of 174
            Pseudo Genes (multiple problems)  :: 20 of 174
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086453.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AE-2247421-T0"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2247421-T0_6"
                     /geo_loc_name="Switzerland"
                     /collection_date="2013"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            join(3796..4052,1..202)
                     /locus_tag="LNN40_RS29605"
                     /old_locus_tag="LNN40_29610"
     CDS             join(3796..4052,1..202)
                     /locus_tag="LNN40_RS29605"
                     /old_locus_tag="LNN40_29610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     gene            complement(710..922)
                     /locus_tag="LNN40_RS29610"
                     /old_locus_tag="LNN40_29615"
     CDS             complement(710..922)
                     /locus_tag="LNN40_RS29610"
                     /old_locus_tag="LNN40_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(926..1411)
                     /locus_tag="LNN40_RS29615"
                     /old_locus_tag="LNN40_29620"
     CDS             complement(926..1411)
                     /locus_tag="LNN40_RS29615"
                     /old_locus_tag="LNN40_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064147840.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYRTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEDGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1449..2126)
                     /locus_tag="LNN40_RS29810"
                     /old_locus_tag="LNN40_29625"
                     /pseudo=""
     CDS             complement(<1449..2126)
                     /locus_tag="LNN40_RS29810"
                     /old_locus_tag="LNN40_29625"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2089..2430)
                     /locus_tag="LNN40_RS29625"
                     /old_locus_tag="LNN40_29630"
     CDS             complement(2089..2430)
                     /locus_tag="LNN40_RS29625"
                     /old_locus_tag="LNN40_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(2518..2697)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          2674..3160
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="93.0"
                     /evalue="5.96e-162"
     Typing          complement(595..2099)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.223"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atttgatcac caagccggaa ctgttttgat aaatggatct caagtacaag cgtcaatttt
       61 agtcactggt ggaggtcaac atgctactgg aaaaacgatc atacaaggtg atacgacaat
      121 gactagccag ggtacaagta taaaaatgtc aggtggtgct caaatagtaa tgactggtgg
      181 agcccgaatc attcagaact aatatgattt accataacat ctcttgcccg caccgctccg
      241 gacatgttcg tcacaaacgg ggcggcagtc aggtaaaacc ggcaacaaac cacccgatac
      301 accgccccgt tcgtgacaga taaattttac ccggctgcat ggtcatgcag ccagatgcat
      361 acggtctgat ttcaggccag aatctggatg aacgccttaa aaacaggaaa atgcttctcc
      421 agccagtccc agatctccag cgccatgtac aggcagttca gcaccgtctc aatcacgtta
      481 aacagtctca taatccctcc gttgtatacg ccgtgatgtt ttcatcacga ccgtatttag
      541 cgggaaattt atgagccctt cttcgtgggg tttgtcactg gtgaagcctg tcagcccagc
      601 gtcggaccgt tatagctccg ctgacgggaa cgggcttccg ccagctcttt ttcgtgctgt
      661 cgctgctcgt atgcccgttc ggcggccacc gccatttcat gccgctgcac tactggctca
      721 aacgctgtaa ctgcctgctc aagttcgcca cctgctgact caagcgcgtc acgcgctcgt
      781 tcagtgccgc gttctcccgt tgcataagcc cagacatctt ctgccattcc gcgaaggcgc
      841 tctcccactc gtccagcctt tgcgagtagt cctgatgtag ctgctctaat gcggtcagca
      901 actgtttttc cagttccgtc atgggctatt tcctctccag taccatccag ttcccgtcct
      961 ccccgtaagc tcccgctttc gggttcactt tgacgcagga cagtttcttc tccccgcaga
     1021 gcgacagctg cacgccgtgg ttcttctccg acagcatggc ctgcgtccgc tcctgcgccc
     1081 ggatagtccg gtaattcccg gtgatctgct gcccctgcca ccacagaatg ctcccgctcg
     1141 tggcgatcag cagtaccgag accagggtga tggtcagcca cgtccggccg accatacgca
     1201 gcacgctcag gcggttcgac tgcatcgccg cggtcatacc ctgctcgtgg gcgctgatgg
     1261 cgtcgctgat tctcttcgcg cttgcgctca gttccgcttt tacagatttc tcgtgctcgc
     1321 tgaatgcggc tttcagcatc tcgccggtac tctgctgctg cgctttcgat tgctgctcca
     1381 agtccttcgc cagcgctaaa agactgctca taaatggctc ccttcagtcg gatgtttcgc
     1441 cccccgtccg ggtcggcaat gctgatactg ttttttgtag tgcggaccac ctcaaaacct
     1501 gccgctgtga gcgcacctgt gacgtcctcg cgagttttga gctccccgga tgaggctaag
     1561 gccagcaacc cacgtgtaat cgcctgagcg gcttccagct tcgtttccgg cagtgcggac
     1621 ggcgtaacca gtaaccgccg gttctccggg gcgtttggat catgcagtcc cagtctgccg
     1681 ttcaccacgg tctgccaggc atcgatgcgc ggccggtcgg cgcgatcgta atacggctgc
     1741 aggcgtttcc cggtcagcag ttcagtgttg gggatcagga aattcagctc cagccgcccc
     1801 ttgtcggtgt gctccaccca cagaatgctg tactggtctt tatcgagccc gggcatcaga
     1861 acccgctcaa agctggccat gatctgctcc cgctgcccgg gcagcagttc agcctccgcg
     1921 aacgacagca cgccggaggt gtactttttc gcatacggcg aggcatcgat aagctcccgg
     1981 acttcctccg gttttccctg cagcacggtc gccccctcgc gctgccggtc ttttcccagc
     2041 aggtaatcca ccggaccggc accaccaccg cgcccccggg gatgaaattt aacgatcatc
     2101 atccccccct ttttccagca ccgcatgccg cagccgctcc agtcccgcgt ctattgccat
     2161 cagggcggcc acaatctgca cccgatcatg accggcacca acggcattca ctttccgggc
     2221 gatctggttc aggttgttac ccataccggc aagctggcgc agcagcgccg gcgatattga
     2281 cggcagtctg caggcacgcg ccggcttctc atcgaggcag gactgacgca tccacgccgc
     2341 cagctgcctg ccgtcgcacc gctcgagcag ccgccggtgt tcgtcttccg tcacccacat
     2401 cgtgagcatc ttgttgcgtt tgtctgccag agtagcctcc tgataacacc gggacgggcg
     2461 ggaaatgttt cccggtcggc acggtgttgg acaagccgca ggcgcgtcag tggcttttag
     2521 cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt atactggcta
     2581 aatatgttac ggcggagtgg taatgtatga aagtgcttca tgtgggaagg ggaaaatgtg
     2641 gctgacgggc gtaagtgcaa catgctggta aaggtgaaat gacgcctcct cgctcactca
     2701 gtcgctgcgc tcctgccgtg agactgcggc gggcgttacc agctcacaga tgacgggata
     2761 cgcaagcagc gctcaaatca ggagaagccg ggaagaggat gcggcgttgc cgtttttcca
     2821 taggctccgc ccccctgacg agtatcataa aatctgacgc tcaaatcagt ggtggcgaaa
     2881 cccgacagga ctataaagat cccaggcgtt tccccctggt agctccctcg tgcgctctcc
     2941 tgttcctgcc attcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     3001 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgatc
     3061 cccccgttca gtccgactac cacgctcttt tcggtaacta tcgtcttgag tccaacccga
     3121 aaaggcacga caaatcgcca ttggcagtag ccactggtaa acaggatgtg cgagagatgt
     3181 attcagagtt cttgaagtgg tggccttact gcggctacac tggaaggaca gctttggtga
     3241 catgtctcgc acaagactgt taccacggtt agtaaatctg ccaggattaa ctaaccttcg
     3301 aaaaaccacc tgccagggtg gttttttcgt ttacagagca aaagactacg ctcagaaaga
     3361 gattatctca agaagatcat ctctttctct tactcaagtc aaagcaaaaa aacaccactc
     3421 catcacaaac tcaggttata gtcggtatgc aactaataac actgtaattt ctgtgcaaaa
     3481 tcgaggttat gcatatgcaa atcaacatca actgcaagag tatcccaaca tctttcgatg
     3541 ttgtctgctc gaattgtgaa tggaccggcg tggctacatt agattatccg gaaaacgtaa
     3601 aaaccctatc tgtcggagga gaaataaatt tcactcataa tgacgagacg ccaaaatgtc
     3661 cacaatgtag cgttggatat atatatggta aaagtggcac ttataaacgt aatagtgcaa
     3721 ctaaccatat ggacagaatc ggagattata aagagtaaat aatacgcatc acagcaatct
     3781 aacgagggaa atataatgat tgaaaccaca ctacttgccg ttctcactgg cgcattaagc
     3841 aatcttactt ctgatgcaat caaaggcatc atagggaagt tagcaagtaa acatcctgaa
     3901 gttgaacaac aagcctcact gcttgaaaca ccattacctt ttgacgaagt tgataaatta
     3961 ttttctcaga tgcagggggc cattcaggct gcagcagcca atggtgaaat tcagctagat
     4021 ggagctgaat taaaagcatt gagcggaata aa
//
