LOCUS       NZ_CP086445             4052 bp    DNA     circular CON 28-MAY-2024
DEFINITION  Klebsiella pneumoniae strain AE-2247421-T5-MAC plasmid
            p2247421-T5-MAC_6, complete sequence.
ACCESSION   NZ_CP086445
VERSION     NZ_CP086445.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22108732
            Assembly: GCF_022759925.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Genome plasticity in E. coli and K. pneumoniae: SNV accumulation
            over time
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Moser,A.I., Endimiani,A., Campos-Madueno,E.I. and Vallone,A.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-NOV-2021) Institute for Infectious Diseases,
            University of Bern, Friedbuehlstrasse 51, Bern 3001, Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 452x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore MiniION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022759925.1-RS_2024_05_28
            Annotation Date                   :: 05/28/2024 04:43:56
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,930
            CDSs (total)                      :: 5,809
            Genes (coding)                    :: 5,641
            CDSs (with protein)               :: 5,641
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 168
            CDSs (without protein)            :: 168
            Pseudo Genes (ambiguous residues) :: 0 of 168
            Pseudo Genes (frameshifted)       :: 64 of 168
            Pseudo Genes (incomplete)         :: 100 of 168
            Pseudo Genes (internal stop)      :: 28 of 168
            Pseudo Genes (multiple problems)  :: 20 of 168
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086445.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="AE-2247421-T5-MAC"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p2247421-T5-MAC_6"
                     /geo_loc_name="Switzerland"
                     /collection_date="2013"
                     /collected_by="Institute for Infectious Diseases Bern"
     gene            69..410
                     /locus_tag="LNN53_RS29500"
                     /old_locus_tag="LNN53_29505"
     CDS             69..410
                     /locus_tag="LNN53_RS29500"
                     /old_locus_tag="LNN53_29505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_057172823.1"
                     /translation="MADKRNKMLTMWVTEDEHRRLLERCDGRQLAAWMRQSCLDEKPAR
                     ACRLPSISPALLRQLAGMGNNLNQIARKVNAVGAGHDRVQIVAALMAIDAGLERLRHAV
                     LEKGGDDDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            619..>1050
                     /locus_tag="LNN53_RS29505"
                     /pseudo=""
     CDS             619..>1050
                     /locus_tag="LNN53_RS29505"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            1088..1573
                     /locus_tag="LNN53_RS29510"
                     /old_locus_tag="LNN53_29515"
     CDS             1088..1573
                     /locus_tag="LNN53_RS29510"
                     /old_locus_tag="LNN53_29515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_064147840.1"
                     /translation="MSSLLALAKDLEQQSKAQQQSTGEMLKAAFSEHEKSVKAELSASA
                     KRISDAISAHEQGMTAAMQSNRLSVLRMVGRTWLTITLVSVLLIATSGSILWWQGQQIT
                     GNYRTIRAQERTQAMLSEKNHGVQLSLCGEKKLSCVKVNPKAGAYGEDGNWMVLERK"
                     /name="MbeB family mobilization protein"
     gene            1577..1789
                     /locus_tag="LNN53_RS29515"
                     /old_locus_tag="LNN53_29520"
     CDS             1577..1789
                     /locus_tag="LNN53_RS29515"
                     /old_locus_tag="LNN53_29520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137423.1"
                     /translation="MTELEKQLLTALEQLHQDYSQRLDEWESAFAEWQKMSGLMQRENA
                     ALNERVTRLSQQVANLSRQLQRLSQ"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(2297..2755)
                     /locus_tag="LNN53_RS29520"
                     /old_locus_tag="LNN53_29525"
     CDS             complement(2297..2755)
                     /locus_tag="LNN53_RS29520"
                     /old_locus_tag="LNN53_29525"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016245712.1"
                     /translation="MIETTLLAVLTGALSNLTSDAIKGIIGKLASKHPEVEQQASLLET
                     PLPFDEVDKLFSQMQGAIQAAAANGEIQLDGAELKALSGIKFDHQAGTVLINGSQVQAS
                     ILVTGGGQHATGKTIIQGDTTMTSQGTSIKMSGGAQIVMTGGARIIQN"
                     /name="hypothetical protein"
     Typing          3855..4034
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.407"
                     /coverage="83.0"
                     /evalue="1.57e-69"
     Typing          complement(3392..3872)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.501"
                     /coverage="92.0"
                     /evalue="5.96e-162"
     Typing          401..1905
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="94.422"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cggcttgtcc aacaccgtgc cgaccgggaa acatttcccg cccgtcccgg tgttatcagg
       61 aggctactct ggcagacaaa cgcaacaaga tgctcacgat gtgggtgacg gaagacgaac
      121 accggcggct gctcgagcgg tgcgacggca ggcagctggc ggcgtggatg cgtcagtcct
      181 gcctcgatga gaagccggcg cgtgcctgca gactgccgtc aatatcgccg gcgctgctgc
      241 gccagcttgc cggtatgggt aacaacctga accagatcgc ccggaaagtg aatgccgttg
      301 gtgccggtca tgatcgggtg cagattgtgg ccgccctgat ggcaatagac gcgggactgg
      361 agcggctgcg gcatgcggtg ctggaaaaag ggggggatga tgatcgttaa atttcatccc
      421 cgggggcgcg gtggtggtgc cggtccggtg gattacctgc tgggaaaaga ccggcagcgc
      481 gagggggcga ccgtgctgca gggaaaaccg gaggaagtcc gggagcttat cgatgcctcg
      541 ccgtatgcga aaaagtacac ctccggcgtg ctgtcgttcg cggaggctga actgctgccc
      601 gggcagcggg agcagatcat ggccagcttt gagcgggttc tgatgcccgg gctcgataaa
      661 gaccagtaca gcattctgtg ggtggagcac accgacaagg ggcggctgga gctgaatttc
      721 ctgatcccca acactgaact gctgaccggg aaacgcctgc agccgtatta cgatcgcgcc
      781 gaccggccgc gcatcgatgc ctggcagacc gtggtgaacg gcagactggg actgcatgat
      841 ccaaacgccc cggagaaccg gcggttactg gttacgccgt ccgcactgcc ggaaacgaag
      901 ctggaagccg ctcaggcgat tacacgtggg ttgctggcct tagcctcatc cggggagctc
      961 aaaactcgcg aggacgtcac aggtgcgctc acagcggcag gttttgaggt ggtccgcact
     1021 acaaaaaaca gtatcagcat tgccgacccg gacggggggc gaaacatccg actgaaggga
     1081 gccatttatg agcagtcttt tagcgctggc gaaggacttg gagcagcaat cgaaagcgca
     1141 gcagcagagt accggcgaga tgctgaaagc cgcattcagc gagcacgaga agtctgtaaa
     1201 agcggaactg agcgcaagcg cgaagagaat cagcgacgcc atcagcgccc acgagcaggg
     1261 tatgaccgcg gcgatgcagt cgaaccgcct gagcgtgctg cgtatggtcg gccggacgtg
     1321 gctgaccatc accctggtct cggtactgct gatcgccacg agcgggagca ttctgtggtg
     1381 gcaggggcag cagatcaccg ggaattaccg gactatccgg gcgcaggagc ggacgcaggc
     1441 catgctgtcg gagaagaacc acggcgtgca gctgtcgctc tgcggggaga agaaactgtc
     1501 ctgcgtcaaa gtgaacccga aagcgggagc ttacggggag gacgggaact ggatggtact
     1561 ggagaggaaa tagcccatga cggaactgga aaaacagttg ctgaccgcat tagagcagct
     1621 acatcaggac tactcgcaaa ggctggacga gtgggagagc gccttcgcgg aatggcagaa
     1681 gatgtctggg cttatgcaac gggagaacgc ggcactgaac gagcgcgtga cgcgcttgag
     1741 tcagcaggtg gcgaacttga gcaggcagtt acagcgtttg agccagtagt gcagcggcat
     1801 gaaatggcgg tggccgccga acgggcatac gagcagcgac agcacgaaaa agagctggcg
     1861 gaagcccgtt cccgtcagcg gagctataac ggtccgacgc tgggctgaca ggcttcacca
     1921 gtgacaaacc ccacgaagaa gggctcataa atttcccgct aaatacggtc gtgatgaaaa
     1981 catcacggcg tatacaacgg agggattatg agactgttta acgtgattga gacggtgctg
     2041 aactgcctgt acatggcgct ggagatctgg gactggctgg agaagcattt tcctgttttt
     2101 aaggcgttca tccagattct ggcctgaaat cagaccgtat gcatctggct gcatgaccat
     2161 gcagccgggt aaaatttatc tgtcacgaac ggggcggtgt atcgggtggt ttgttgccgg
     2221 ttttacctga ctgccgcccc gtttgtgacg aacatgtccg gagcggtgcg ggcaagagat
     2281 gttatggtaa atcatattag ttctgaatga ttcgggctcc accagtcatt actatttgag
     2341 caccacctga catttttata cttgtaccct ggctagtcat tgtcgtatca ccttgtatga
     2401 tcgtttttcc agtagcatgt tgacctccac cagtgactaa aattgacgct tgtacttgag
     2461 atccatttat caaaacagtt ccggcttggt gatcaaattt tattccgctc aatgctttta
     2521 attcagctcc atctagctga atttcaccat tggctgctgc agcctgaatg gccccctgca
     2581 tctgagaaaa taatttatca acttcgtcaa aaggtaatgg tgtttcaagc agtgaggctt
     2641 gttgttcaac ttcaggatgt ttacttgcta acttccctat gatgcctttg attgcatcag
     2701 aagtaagatt gcttaatgcg ccagtgagaa cggcaagtag tgtggtttca atcattatat
     2761 ttccctcgtt agattgctgt gatgcgtatt atttactctt tataatctcc gattctgtcc
     2821 atatggttag ttgcactatt acgtttataa gtgccacttt taccatatat atatccaacg
     2881 ctacattgtg gacattttgg cgtctcgtca ttatgagtga aatttatttc tcctccgaca
     2941 gatagggttt ttacgttttc cggataatct aatgtagcca cgccggtcca ttcacaattc
     3001 gagcagacaa catcgaaaga tgttgggata ctcttgcagt tgatgttgat ttgcatatgc
     3061 ataacctcga ttttgcacag aaattacagt gttattagtt gcataccgac tataacctga
     3121 gtttgtgatg gagtggtgtt tttttgcttt gacttgagta agagaaagag atgatcttct
     3181 tgagataatc tctttctgag cgtagtcttt tgctctgtaa acgaaaaaac caccctggca
     3241 ggtggttttt cgaaggttag ttaatcctgg cagatttact aaccgtggta acagtcttgt
     3301 gcgagacatg tcaccaaagc tgtccttcca gtgtagccgc agtaaggcca ccacttcaag
     3361 aactctgaat acatctctcg cacatcctgt ttaccagtgg ctactgccaa tggcgatttg
     3421 tcgtgccttt tcgggttgga ctcaagacga tagttaccga aaagagcgtg gtagtcggac
     3481 tgaacggggg gatcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     3541 gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaat
     3601 ggcaggaaca ggagagcgca cgagggagct accaggggga aacgcctggg atctttatag
     3661 tcctgtcggg tttcgccacc actgatttga gcgtcagatt ttatgatact cgtcaggggg
     3721 gcggagccta tggaaaaacg gcaacgccgc atcctcttcc cggcttctcc tgatttgagc
     3781 gctgcttgcg tatcccgtca tctgtgagct ggtaacgccc gccgcagtct cacggcagga
     3841 gcgcagcgac tgagtgagcg aggaggcgtc atttcacctt taccagcatg ttgcacttac
     3901 gcccgtcagc cacattttcc ccttcccaca tgaagcactt tcatacatta ccactccgcc
     3961 gtaacatatt tagccagtat acactccgct agcgctacgt gactggttca gggctgcgcc
     4021 ccgaaacccg ctaaaagcca ctgacgcgcc tg
//
