LOCUS       NZ_CP086290             6874 bp    DNA     circular CON 31-MAR-2024
DEFINITION  Citrobacter freundii strain DY2010 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP086290
VERSION     NZ_CP086290.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21601121
            Assembly: GCF_020809005.1
KEYWORDS    RefSeq.
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1  (bases 1 to 6874)
  AUTHORS   Ye,J., Jin,L., Li,Y., Xu,H., Ye,W., Lin,Y., Zheng,B. and Zhou,T.
  TITLE     Complete-genome sequencing and comparative genomic characterization
            of blaNDM-5 producing Citrobacter freundii isolates from a patient
            with multiple infections
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6874)
  AUTHORS   Ye,J., Jin,L., Li,Y., Xu,H., Ye,W., Lin,Y., Zheng,B. and Zhou,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2021) Department of Clinical Laboratory, The First
            Affiliated Hospital of Wenzhou Medical University, nanbaixiang,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SPAdes v. 3.9.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200X
            Sequencing Technology             :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020809005.1-RS_2024_03_31
            Annotation Date                   :: 03/31/2024 06:15:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,107
            CDSs (total)                      :: 4,987
            Genes (coding)                    :: 4,874
            CDSs (with protein)               :: 4,874
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 113
            CDSs (without protein)            :: 113
            Pseudo Genes (ambiguous residues) :: 0 of 113
            Pseudo Genes (frameshifted)       :: 31 of 113
            Pseudo Genes (incomplete)         :: 83 of 113
            Pseudo Genes (internal stop)      :: 18 of 113
            Pseudo Genes (multiple problems)  :: 19 of 113
            Pseudo Genes (short protein)      :: 1 of 113
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086290.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6874
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="DY2010"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:546"
                     /plasmid="unnamed3"
                     /geo_loc_name="China: Dongyang"
                     /lat_lon="29.29 N 120.24 E"
                     /collection_date="2020-01-24"
                     /collected_by="Affiliated Dongyang Hospital of Wenzhou
                     Medical University"
     gene            62..1432
                     /locus_tag="LMH92_RS25455"
                     /old_locus_tag="LMH92_25490"
     CDS             62..1432
                     /locus_tag="LMH92_RS25455"
                     /old_locus_tag="LMH92_25490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971921.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISCfr1 family transposase"
                     /protein_id="WP_000971921.1"
                     /translation="MMGQLPGGQQRLFYSFNLEDHVPAQHLLRSIDQCLDLSDLRAYLA
                     DFYSPIGRPSIDPELMVRMLVVGYCYGIRSERRLCEEVHLNLAYRWFCRLGLEDEVPNH
                     STFSKNRHGRFRDSDLFRWLFNEVLRRCMAAGLVKGEGFAVDASIIKADASRQRGVAGD
                     EVDWNDPKLSSRAVREYLEALDEEALAEALPKKISLTDPQSRWTAAPGGPAFFAYSTNY
                     LIDTEHGVIMDVEATPAHRTAEVDSTRTMVERVEAQFDLTPERLIGDTAYGTAPMLAWM
                     VEEKDIEPHVPVWDKTERKDDSLSSNDFHWSQDANEYRCPAGKPLRSEWRAFTQQRSRV
                     TKAKTVIYRSSQTDCATCPLKAKCCPNTPNRKIVRSIHEAARDVARRIAKTPEYLVSRC
                     ERKKVEMLFAHLKRIMKLDRLRLRGLTGATDEFTLAAMVQNLRRMAKLLPQGPPLTG"
                     /name="IS1182-like element ISCfr1 family transposase"
     gene            2253..3113
                     /locus_tag="LMH92_RS25460"
                     /old_locus_tag="LMH92_25495"
     CDS             2253..3113
                     /locus_tag="LMH92_RS25460"
                     /old_locus_tag="LMH92_25495"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="WP_000027057.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
                     /name="broad-spectrum class A beta-lactamase TEM-1"
     gene            3295..4155
                     /gene="aac(3)-IId"
                     /locus_tag="LMH92_RS25465"
                     /old_locus_tag="LMH92_25500"
     ARG             3296..4152
                     /name="aac(3)-IId"
                     /gene="aac(3)-IId"
                     /gene_name="aminoglycoside N-acetyltransferase AAC(3)-IId"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            4168..4710
                     /locus_tag="LMH92_RS25470"
                     /old_locus_tag="LMH92_25505"
     CDS             4168..4710
                     /locus_tag="LMH92_RS25470"
                     /old_locus_tag="LMH92_25505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_000587837.1"
                     /translation="MIIWINGPFGAGKTTLAKRLRDRRSKSLIFDPEEIGFVVKETVPM
                     PASGDYQDLPLWRGLTIAAVREIRRNYSQDIIIPMTLVHPDYLTEILDGVRRIDDQLLH
                     IFLTLNEDLLRHRIANQTMHPDPNRNAEIREWRLANVARCLAARERLPCTTRVLDSGAH
                     TSDELAAMVLDGIDGRT"
                     /name="AAA family ATPase"
     gene            complement(5192..5383)
                     /locus_tag="LMH92_RS25475"
                     /old_locus_tag="LMH92_25510"
     CDS             complement(5192..5383)
                     /locus_tag="LMH92_RS25475"
                     /old_locus_tag="LMH92_25510"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000951934.1"
                     /translation="MAIALMGAGFSATDTSDAVNILYPITMTVQANKAWQASGLKKSFF
                     LPSHKKVRGGEKISYARL"
                     /name="hypothetical protein"
     gene            5407..5634
                     /locus_tag="LMH92_RS25480"
                     /old_locus_tag="LMH92_25515"
     CDS             5407..5634
                     /locus_tag="LMH92_RS25480"
                     /old_locus_tag="LMH92_25515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000248278.1"
                     /translation="MVNSYSLSDDSGVMAAAAITHFLFGQAVFSYLNGWSVLIGPGTGL
                     DSTGCKYARDLMGLVAFTAFIVTFLFRGYS"
                     /name="hypothetical protein"
     gene            5685..6821
                     /locus_tag="LMH92_RS25485"
                     /old_locus_tag="LMH92_25520"
     CDS             5685..6821
                     /locus_tag="LMH92_RS25485"
                     /old_locus_tag="LMH92_25520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="WP_000080861.1"
                     /translation="MSQLSQLRSPAAVQAAIDEFVQLGRTKFLARHGYGKSRDFLVRDP
                     KTGTDCDSKAIAGVAFGKQFPEQGPLTADSFSGGETTVVPALTRLGFRIIRIGEDWSEE
                     EVLATVEDYFDMLRAEAAGEPYHKSEHNQALRQLLNGRSKSSVELKHQNISAVLDALGL
                     PYINGYKPRGNSQLLLRKSVHAYVLEHQQTVGALVDALEEVKLPGDKTYRAALVEPPAR
                     EVLVRTPASLRQRLPRKFDYAARDEANRKLGRAGEQWVIGYEQQRLTELGHPELFQRLD
                     WVSDTQGDGAGFDILSFEEDAHERFIEVKTTNGGVGSSFLVSHNELEFSKEAGDQFHLY
                     RVFQFRDGPRLFTLPGDLSQHVHLKPTDYRASFRSLVG"
                     /name="DUF3883 domain-containing protein"
     ARG             2254..3110
                     /name="blaTEM-1"
                     /gene="blaTEM-1"
                     /gene_name="broad-spectrum class A beta-lactamase TEM-1"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 gtagcggcct ccccatgccg ttggcaaaat tgctttgtca gcgagcgtgg gggcgaacag
       61 catgatggga cagttaccgg gaggacagca gcgcctgttc tactcgttca atctggaaga
      121 tcacgtcccg gcccaacatc tcctgcgcag catcgaccag tgcttggatc tcagtgatct
      181 acgtgcctac ctggcagatt tctatagccc catcgggcgt ccctcgattg acccggagtt
      241 gatggtgcgc atgctggtcg tcggctactg ctatggcatt cgttccgagc ggcgattgtg
      301 cgaagaggtg cacctgaacc tggcctatcg ctggttctgc cggttgggtc tggaagacga
      361 agtccccaat cactcgacct tctcgaagaa tcgccatggg cgttttcgtg acagcgatct
      421 attccgctgg ttattcaatg aggtgctgcg gcgctgcatg gcagccggcc tagtcaaggg
      481 tgaaggtttc gccgtcgacg ccagcatcat taaggcggat gccagccggc aacgtggggt
      541 ggcgggagat gaggtcgatt ggaacgatcc aaagctcagc agccgcgcag tgcgcgagta
      601 cctcgaagcc cttgatgaag aggcgctggc tgaggctctt cccaagaaaa tttcgctcac
      661 tgatcctcag tcccgttgga cagcagcgcc aggtggcccg gccttttttg cctactccac
      721 gaattacctg atcgacactg agcacggtgt gatcatggac gtggaagcta ccccggcgca
      781 ccgtaccgcc gaagtcgatt cgactaggac gatggtcgag cgtgtcgagg cgcagttcga
      841 tctcacaccg gaacgcctta tcggcgatac cgcttatggc accgccccga tgctggcctg
      901 gatggtcgaa gaaaaggaca tcgaaccgca tgtgccggtg tgggacaaga ccgagcgcaa
      961 ggacgacagc ctctccagta acgactttca ctggagtcag gacgccaatg aatatcgctg
     1021 cccagccggc aaaccgctac gcagtgaatg gcgcgccttc acccagcaaa ggtcgcgggt
     1081 aactaaggcc aaaaccgtca tttaccgctc cagccaaacc gactgcgcca cctgcccgtt
     1141 gaaagcgaaa tgctgcccca acacgccgaa tcggaagatc gtccgcagca tccatgaggc
     1201 tgcccgcgac gtggctcgac gcatcgccaa gacaccggag tacctcgtct ctcgctgcga
     1261 acgaaagaag gtggagatgc ttttcgccca cctcaaacgg atcatgaaac tcgaccgttt
     1321 acgactgcgt ggcctaacgg gtgccactga cgaattcacc ttggctgcga tggtgcagaa
     1381 cctgcgccgc atggccaagc ttttgcctca agggccaccg ctgacgggat aggtatgcct
     1441 gctacgagca gaaaccctca aattaaccct taaacctgag caaggacgct cagtgaaacg
     1501 ccggaaggca acttgaagtg gcttgcagcc acttcgacag caggcacacc tgatcggcag
     1561 gctgccgcta aagctacttt ttcaacagaa tcggccgatt tgtgccggtc gtcaaggacc
     1621 gcttcggatc actctcaacc ggatcgcggg ccagctgatt tgctaaaacc cgcgccaaac
     1681 tcaacgaagg atttcgcatg atccgttttt tttggcgatc acgctcggcc tgatggttgg
     1741 ctgctcgtcg agttcaagtt cgatctgatc agtgagcggt agcgcaagtg gatcgcctca
     1801 gccaagcagc gttgttgccg gcggccggaa gaaagccttg aactagtagc ggaaaggatg
     1861 aaactttatt tttacgattc tcagcatttt ccaaggttga gcgaaaatta ggatgatact
     1921 ttaatgattc cggcctccat ccccacaaaa cgcttgcaga tggcagaatt gaaataagtc
     1981 attgttttaa atggattttt gcaggaattg ctacgttgca gggtcctgta gttaggatga
     2041 cactttattt tcctcccaca ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     2101 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     2161 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctggt
     2221 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttt cgtgtcgccc
     2281 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     2341 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     2401 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2461 ttaaagttct gctatgtggt gcggtattat cccgtgttga cgccgggcaa gagcaactcg
     2521 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2581 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2641 acactgctgc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2701 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2761 ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca
     2821 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2881 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2941 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     3001 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     3061 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     3121 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     3181 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     3241 tccactgagc gtcagacccc gtatagtgtt ttgcagttta gaggagatat cgcgatgcat
     3301 acgcggaagg caataacgga ggcgcttcaa aaactcggag tccaaaccgg tgacctcttg
     3361 atggtgcatg cctcacttaa agcgattggt ccggtcgaag gaggagcgga gacggtcgtt
     3421 gccgcgttac gctccgcggt tgggccgact ggcactgtga tgggatacgc gtcgtgggac
     3481 cgatcaccct acgaggagac tctgaatggc gctcggctgg atgacgaagc ccgccgtacc
     3541 tggctgccgt tcgatcccgc aacagccggg acttaccgtg ggttcggcct gctgaatcaa
     3601 tttctggttc aagcccccgg cgcgcggcgc agcgcgcacc ccgatgcatc gatggtcgcg
     3661 gttggtccgc tggctgaaac gctgacggag cctcacgaac tcggtcacgc cttgggggaa
     3721 ggatcgcccg tcgagcggtt cgttcgcctt ggcgggaagg ccctgctgtt gggtgcgccg
     3781 ctaaactccg ttaccgcatt gcactacgcc gaggcggttg ccgatatccc caacaaacgg
     3841 tgggtgacgt atgagatgcc gatgcttgga agagacggtg aagtcgcctg gaaaacggca
     3901 tcggattacg attcaaacgg cattctcgat tgctttgcta tcgaaggaaa gccggatgcg
     3961 gttgaaacta tagcaaatgc ttacgtgaag ctcggtcgcc atcgagaagg tgtcgtgggc
     4021 tttgctcagt gctacctgtt cgacgcgcag gacatcgtga cgttcggcgt cacctatctt
     4081 gagaagcatt tcggaaccac tccgatcgtg cctccgcacg aggccgtcga gcgctcttgc
     4141 gagccttcag gttagaggcc gtcgacaatg ataatctgga tcaacggacc tttcggcgcc
     4201 ggaaagacga cgctcgctaa gcggctgcgc gatcggcgtt ccaaatcgct gatctttgac
     4261 cccgaggaaa tcgggttcgt ggtgaaagaa acggtcccca tgccagcgag cggagactat
     4321 caggatctcc ccttgtggag gggacttacg atcgcggcgg tcagggagat tcgaaggaat
     4381 tactcgcagg acatcatcat cccaatgacg ctcgtgcacc cggactatct gactgagata
     4441 ctcgacgggg taaggcggat cgacgatcag ctgctgcaca tctttctgac gctcaacgag
     4501 gacctattgc gtcaccggat cgcgaaccag accatgcatc ctgacccgaa tcgaaatgcg
     4561 gagattcgag agtggcgatt agcgaatgtc gcccgatgct tggccgcaag ggaacggctt
     4621 ccatgcacaa cccgtgttct cgatagtggt gcacacacca gcgatgaact cgcagcgatg
     4681 gtgctcgacg gaatcgatgg gcgcacctga tcgccttcga cgcctgcgca aagcgtagcg
     4741 cgagggtggc gggctcacga ccaaacgccc agaggtcgat catcgcaggg atgtttggct
     4801 ttgtggtgcg gacgacggga ctcgaacccg tactctcaca gagaagcaga ttttcgtacc
     4861 acctcgactt tcgccgccgt ctgatgacgt tcgtggtctg gactgtccct tcgccattgc
     4921 ccgaaggctt taggcgccgc ccgtccagtc tctacacctt cccccgaagg ggcttggctc
     4981 gggattggct tagggtattg cccgttagcg ttccccgact ttgagcggtt ctactccgcg
     5041 gatttccccg cgggcactcc aattttaaag tctgctgcgt ctaccgattt cgccacgtcc
     5101 gccttttttc gccgttccta gcgctcgtgc gatgcaccta tgttgcacct agcgccgaat
     5161 cgttcttcgt catcctgaaa aaccacgtct cctaaagcct tgcatagctt atcttttctc
     5221 caccacgaac ttttttgtgg gatggtagaa aaaaagactt tttaagtccg ctggcttgcc
     5281 aggccttgtt agcttgtacg gtcatggtta tcgggtaaag aatattgacg gcatcgctgg
     5341 tgtcggtggc tgaaaagccg gctcccatca gggcaatagc catttcagat gcaggcgtac
     5401 agggcaatgg tcaacagcta cagcctgtct gacgattccg gcgtcatggc tgcggcggct
     5461 atcacgcatt ttttgttcgg tcaggcggtg ttttcgtacc tcaatggttg gagcgtgttg
     5521 atcggacctg gtacaggttt ggacagcacg ggctgcaaat acgcaaggga tttaatgggc
     5581 ctggtggcgt tcacggcttt tatcgtgacg tttctgttca ggggctactc ataatctcgt
     5641 ggctcggcgg ttcccggcac accatgacag taaggaagga ccctgtgtct caactctccc
     5701 agcttcgaag ccccgccgcc gtgcaggctg ccatcgatga gttcgtgcaa ctgggccgca
     5761 cgaaattcct ggcgcgccac ggctacggca agtcccgcga cttcctggta cgtgatccga
     5821 agaccggcac cgattgcgat tccaaggcca tcgccggtgt ggccttcggc aagcaatttc
     5881 ccgagcaggg cccgctcact gctgacagct tctccggtgg cgagacgacc gtcgttccgg
     5941 cgctgacgcg gctcgggttt cgcatcattc gcatcggcga agactggtcc gaagaagagg
     6001 tcctggccac ggtcgaagac tatttcgaca tgctgcgtgc cgaggcggct ggggagccgt
     6061 accacaagtc cgagcacaac caggcactgc gccaactgct gaacggtcgc agcaagtctt
     6121 cagtcgagct caagcaccag aacattagcg ccgtactcga tgccctgggc ctgccctata
     6181 tcaacggcta caagccacgc ggcaacagcc aactgctgct gcgtaaatcc gtacacgcct
     6241 acgttctgga acatcagcag acggtcggcg ctcttgtcga tgccctggag gaggtaaaac
     6301 ttccgggtga caaaacctac cgagcggctt tggtagaacc acccgcccgt gaagtgcttg
     6361 tgcgtacccc ggcatctcta cggcaacgcc taccgcgaaa gttcgattat gccgctcgcg
     6421 atgaagccaa ccgcaagctg ggccgggcag gggagcagtg ggtgattggc tacgaacagc
     6481 aacgcctgac cgagctcggc cacccagagc tttttcagcg gctggattgg gtgtccgaca
     6541 cccagggaga cggtgcgggg ttcgacatcc tgtcgttcga agaggacgcc catgagcgct
     6601 tcatcgaggt gaaaaccacc aatggcgggg taggctcgtc tttcttggtc agccacaacg
     6661 aactcgaatt ctccaaggag gcgggcgatc aattccatct gtatcgcgtg ttccagtttc
     6721 gggacggtcc gcgcctgttc acgctacccg gcgacctcag ccaacatgtg catctcaagc
     6781 cgacggacta ccgggcgagt ttccggagtt tggtggggta aaggcagggt tctgttgagc
     6841 cgaatggctg tgtgcggccg attctgttga aaaa
//
