LOCUS       NZ_CP086264             6728 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Escherichia coli strain SCPM-O-B-9427 plasmid pB-9427-5, complete
            sequence.
ACCESSION   NZ_CP086264
VERSION     NZ_CP086264.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22830670
            Assembly: GCF_020808965.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6728)
  AUTHORS   Kartsev,N., Kislichkina,A., Teymurazov,M., Kuzina,E., Kanashenko,M.,
            Skryabin,Y., Sizova,A., Bogun,A. and Svetoch,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow Region 142279, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 53.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 14:31:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,437
            CDSs (total)                      :: 5,312
            Genes (coding)                    :: 5,008
            CDSs (with protein)               :: 5,008
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 304
            CDSs (without protein)            :: 304
            Pseudo Genes (ambiguous residues) :: 0 of 304
            Pseudo Genes (frameshifted)       :: 124 of 304
            Pseudo Genes (incomplete)         :: 198 of 304
            Pseudo Genes (internal stop)      :: 62 of 304
            Pseudo Genes (multiple problems)  :: 69 of 304
            Pseudo Genes (short protein)      :: 2 of 304
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086264.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCPM-O-B-9427"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB-9427-5"
                     /geo_loc_name="Russia: Saint-Petersburg"
                     /collection_date="2018-07-30"
     gene            join(6393..6728,1..558)
                     /locus_tag="LMH79_RS26990"
                     /old_locus_tag="LMH79_27015"
     CDS             join(6393..6728,1..558)
                     /locus_tag="LMH79_RS26990"
                     /old_locus_tag="LMH79_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001277131.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQP
                     HALYWLPFDVDRMGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASASA
                     LRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLSASA
                     RRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPVPLSP
                     AECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWEALGIS
                     RATYYRKLKCDPDLAK"
                     /name="replication initiation protein"
     gene            complement(774..1049)
                     /locus_tag="LMH79_RS26995"
                     /old_locus_tag="LMH79_27020"
     CDS             complement(774..1049)
                     /locus_tag="LMH79_RS26995"
                     /old_locus_tag="LMH79_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000091753.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRRP
                     EEAQLRAFLLQFLRKEVTRQADVNRLQPLINELEMLPRPPAKPQNH"
                     /name="hypothetical protein"
     gene            1228..3447
                     /gene="mobQ"
                     /locus_tag="LMH79_RS27000"
                     /old_locus_tag="LMH79_27025"
     CDS             1228..3447
                     /gene="mobQ"
                     /locus_tag="LMH79_RS27000"
                     /old_locus_tag="LMH79_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_052979306.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTRK
                     RGVDDAVILTPANAPSWCSDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAREA
                     VLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNERALAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETERGEQ
                     NRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAANASADTLRE
                     FIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKARPVASL
                     LSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFETQWNTWIA
                     PGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETALVGVITELG
                     RAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREG
                     MPESHETMARPERMRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCPV
                     LVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLLAA
                     VHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
                     /name="mobQ"
     gene            3680..5395
                     /gene="col"
                     /locus_tag="LMH79_RS27005"
                     /old_locus_tag="LMH79_27030"
     CDS             3680..5395
                     /gene="col"
                     /locus_tag="LMH79_RS27005"
                     /old_locus_tag="LMH79_27030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_172690082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-E2"
                     /protein_id="WP_052979305.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENNP
                     WGGGSGHGNGGGNSNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGGLAVSISAGALSA
                     AIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLPADDITESPVSSLP
                     LDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPGVFTASIPGAPVLNI
                     SVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPKDSGHNAVYVSVSDVL
                     SPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQANEDVARNQERQAKAVQV
                     YNSRKSELDAANKTLADAIAEIKQFDRFAHDPMAGGHRMWQMAGLKAQRAQTDVNNKQA
                     AFDAAAKEMSDADAALSAAQERRKQKENKEKDAKDKLDKESKRNKPGKATGKGKPVGDK
                     WLDDAGKDSGAPIPDRIADKLRDKEFKNFDDFRKKFWEEVSKDPDLSKQFKGSNKTNIQ
                     KGKAPFARKKDQVGGRERFELHHDKPISQDGGVYDMNNIRVTTPKRHIDIHRGK"
                     /name="col"
     gene            5398..5658
                     /locus_tag="LMH79_RS27010"
                     /old_locus_tag="LMH79_27035"
     CDS             5398..5658
                     /locus_tag="LMH79_RS27010"
                     /old_locus_tag="LMH79_27035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="WP_000420693.1"
                     /translation="MELKHSISDYTEAEFLEFVKKICRAEGATEEDDNKLVREFERLTE
                     HPDGSDLIYYPRDDREDSPEGIVKEIKEWRAANGKSGFKQG"
                     /name="bacteriocin immunity protein"
     gene            5720..5863
                     /locus_tag="LMH79_RS27015"
                     /old_locus_tag="LMH79_27040"
     CDS             5720..5863
                     /locus_tag="LMH79_RS27015"
                     /old_locus_tag="LMH79_27040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_000724383.1"
                     /translation="MKKITGIILLLLAVIILSACQANYIRDVQGGTVSPSSTAEVTGLA
                     TQ"
                     /name="colicin release lysis protein"
     BGC             1..6728
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(1054..1227)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
     Typing          complement(1228..1586)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          86..558
                     /name="rep_cluster_2150"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6394..6728
                     /name="rep_cluster_2141"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.262"
                     /coverage="100.0"
                     /evalue="1.11e-131"
     Typing          1229..3444
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.512"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgtgcgttgt gtgaaaaact gggcgcggat gtgaattaca gcggcctgat ctgcaaaaat
       61 ccgtgccacc ctgaatggca ggaagtggaa tggcgcgagg aaccctacac tctcgacgaa
      121 ctggctgatt atctcgattt gagcgcctca gcgcgccgta gcgtcgataa aaattacggg
      181 ctggggcgaa actaccatct gttcgaaaag gtccgtaaat gggcctacag agcgattcgt
      241 cagggctggc ctgtattctc acaatggctt gatgccgtga tccagcgtgt cgaaatgtac
      301 aacgcatcgc ttcccgttcc gctttctccg gctgaatgtc gggctattgg caagagcatt
      361 gcgaaatata cacacaggaa attctcacca gagggatttt ccgctgtaca ggccgctcga
      421 ggtcgcaagg gcggaactaa atctaagcgc gcagcagttc ctacatcagc acgttcgctg
      481 aagccgtggg aggcattagg gatcagtcga gcgacgtact accgaaaatt aaaatgtgat
      541 ccagatctcg caaaatgaga ccagataagc cttatcagat aacagcgccc ttttggcgtc
      601 tttttgagca gtgacctttc ttgcgatttt ctccgttctg ccccgtaatg gcgtggcttt
      661 ccgcatgatt gaggtggtag cgctcgccgc agtctcatga ccgagcgtag cgagcgaatg
      721 agcgaggaag cgcaaaggcg tccggtggtg catgggcact tacgcgccgg ggcttagtgg
      781 ttctgcggtt tcgccggtgg tctgggtagc atctccagct cgttaatcag cggttgtagt
      841 cggttcacat ccgcctgtct tgtgacttcc tttcgcagaa actggagcag gaacgcacgc
      901 agttgcgctt cttccggcct ccgtaccctc gccagcatgg cagcccccac aatgactttt
      961 tgcgccgtgt ccaggctccg gctcttcgcc ttcaggcgct gtaatctggc ctcagcttcg
     1021 gcaatcttct gttcgagtgt tctgctcatt tcgtgactcc gtgcgcggtg aaaaatcgca
     1081 ttttagcgcg tcactggtag ttgcaaaact gaactggcat aatgcacggc acatcacgaa
     1141 gtgcgcactt atacaatctc tactccgttt cgattgtgtg cggtctgcga cgctaaaaga
     1201 aaacggcaaa aaggtattac ggcagaaatg gcgatttatc atctcagcat gaaaatcatt
     1261 tcgcgaaaaa acggctacag tgccgttgct tctgctgcct accgttccgg ctctgtcata
     1321 cccgatgacc gtaccggatt aacccacgat tacacccgta aacgcggcgt tgatgatgcg
     1381 gtcattctca cccctgcgaa tgcaccgtcc tggtgtagtg accgttccgt tctctggaat
     1441 gcggtcgaga aagccgaaca gcgccggaac tcccagctgg caagggagat tgaactcgcc
     1501 attccccgtg agatttcccg cgaggccgca cgggaggccg ttctcgcttt tgtccgggaa
     1561 aactttgtca gtcggggcat gattgccgat gtggcgtttc atcacatgga ccggaccaat
     1621 ccccatgcgc acatcatgct gaccacgaga gctgtcgggg aaacaggatt tgcaggaaag
     1681 gtcagggact ggaacgagcg ggcactcgcc gagacgtggc gcgcatcatg ggctgaccat
     1741 gcgaacagag cgcttgcgaa cgccggctac caggaagaga tagaccatcg ttcatacgag
     1801 cgtcagggac tggagaaagc gccgggcctt cacctcggaa aggctgcctg tgcgatggaa
     1861 aaacggggca tggaaacaga acgcggtgag cagaaccgcc tgattaacag ccttaacctg
     1921 gaaatacagg tttcccgcac aaggctggca gtcatccagg tacaggagaa cgtgcggaaa
     1981 cgggaactca gcgatgccgc acgtcgtgca gcggaagccc ttaacctgac cattcccgct
     2041 gcgaatgcct cagcggatac cctgcgggaa ttcattgcca cgctgccgca ggaatgcggg
     2101 aacgcgtggg agatgacccc ggagttcctg gcgatgagcg ggaaggtgaa cgacatcgaa
     2161 cgtgagggga atgcgctgct gaaagagcag gccattctcg aaaaggagat gaccggactc
     2221 aaaaaagcac gccctgtcgc gtcccttctg tcagagattc ccctgatgac atgggctgaa
     2281 ccggaatacc gcaaaagaca actccgtttc tggaaactcg ggaaacagat tgaatctctt
     2341 cgccgcacct acagggccgt gaaagaacgg gacattcccg cccgtcgtca ggcctttgaa
     2401 acgcagtgga atacgtggat tgcgccggga atggcagagc tgaaagaaaa actgtcagca
     2461 cgggaagcgg agcggcgcag ggaggaagcc gaagcggaag cgcgccggaa ggaacaggag
     2521 catgaggcgc ggctgaaacg tcatgataac caccgtctga gccgtgaaac ggcattagtc
     2581 ggggttatta cggagctggg gcgtgccaga gagccgggaa cgggcaggat aacccgctac
     2641 atgatgttga gtaacagagc cggagaattc acggtatggg gtgatgagct ggcgcattac
     2701 ccccagagtg ttcatgaccc ggtgaatgtt tacctgtcgc caggcggggc tgtgatggtc
     2761 tcggatatac gcgagggaat gccagaatct catgagacga tggcgcggcc tgagcgtatg
     2821 agaatgtatt ccggtgcgac ggtccggcat gtactggaac agatgcgcca ggggtggccc
     2881 tcttacggtt ttccggcgct gccgcatcac tggccggata atttttattt cagcgacgac
     2941 cgcaggcccg tagcctctcc gctgccgtca gcgcaccggg tggatatcac cgctgatgct
     3001 gtaccggaac aactcatgcc tgttgtattt tcgagagagg gaaacagcag gacgctgaat
     3061 ctgttgttat gcaaagggcc ggaggaaatg cttgtcgggt ttgttcgccg ggaaggaggg
     3121 ttttgccccg ttcttgtgct tccgtcgccg gattacagtc atctgatagt cagcaccatc
     3181 acggagaacg gggtatgcct ggcaggttac ggagaagcta taaaccatga tgaggatact
     3241 ccgtacccac cggaaccgca cctgatgcag ttccggctca aaggccatca tgacaggctt
     3301 ttggctgctg tccacaaacc ggaagagatg ccggattatc tcttccgtca actcggtttt
     3361 aatcagacct ggcatgagtg gaagcgggac gaacagcaca ggcaacaaca acgccgcccc
     3421 gggcacttcc ggggcatgag tatgtgatat ccggggctgc cccccggacc ccgccaacac
     3481 atcacgggcc acaaaatttt ttgtggcccg ctctgcgttt tctaagtgtt atccctcctg
     3541 atttctaaaa aattttccac ctgaacttga cagaaaaaac gatgacgggt actttttgat
     3601 ctgtacataa aaccagtggt tttatgtaca gtattgatca tgtaattaat tgttttaacg
     3661 cttaaaagag ggagttttta tgagcggtgg cgatggacgc ggccataaca cgggcgcgca
     3721 tagcacaagt ggtaacatta atggtggccc gaccgggctt ggtgtaggtg gtggtgcttc
     3781 tgatggttcc gggtggagtt cggaaaataa cccgtggggt ggtggttccg gtcatggtaa
     3841 tggcgggggg aatagtaatt ccggtggtgg ctcgggaaca ggcggtaatc tgtcagcagt
     3901 agctgcgcca gtggcatttg gttttccggc actttccact ccaggagctg gcggtctggc
     3961 ggtcagtatt tcagcgggag cattatcggc agctattgct gatattatgg ctgccctgaa
     4021 aggaccgttt aaatttggtc tttggggggt ggctttatat ggtgtattgc catcacaaat
     4081 agcgaaagat gaccccaata tgatgtcaaa gattgtgacg tcattacccg cagatgatat
     4141 tactgaatca cctgtcagtt cattacctct cgataaggca acagtaaacg taaatgttcg
     4201 tgttgttgat gatgtaaaag acgaacgaca gaatatttcg gttgtttcag gtgttccgat
     4261 gagtgttccg gtggttgatg caaaacctac cgaacgtcca ggtgttttta cggcatcaat
     4321 tccaggtgca cctgttctga atatttcagt taataacagt acgccagcag tacagacatt
     4381 aagcccaggt gttacaaata atactgataa ggatgttcgc ccggcaggat ttactcaggg
     4441 tggtaatacc agggatgcag ttattcgatt cccgaaggac agcggtcata atgccgtata
     4501 tgtttcagtg agtgatgttc ttagccctga ccaggtaaaa caacgtcagg atgaagaaaa
     4561 tcgccgtcag caggaatggg atgctacgca tccggttgaa gcggctgagc gaaattatga
     4621 acgcgcgcgt gcagagctga atcaggcaaa tgaagatgtt gccagaaatc aggagcgaca
     4681 ggctaaagct gttcaggttt ataattcgcg taaaagcgaa cttgatgcag cgaataaaac
     4741 tcttgctgat gcaatagctg aaataaaaca atttgatcga tttgcccatg acccaatggc
     4801 tggcggtcac agaatgtggc aaatggccgg gcttaaagcc cagcgggcgc agacggatgt
     4861 aaataataag caggctgcat ttgatgctgc tgcaaaagag atgtcagatg ctgatgctgc
     4921 attaagtgcc gcgcaggagc gccgcaaaca gaaggaaaat aaagaaaagg acgctaagga
     4981 taaattagat aaggagagta aacggaataa gccagggaag gcgacaggta aaggtaaacc
     5041 agttggtgat aaatggctgg atgatgcagg taaagattca ggagcgccaa ttccagatcg
     5101 cattgctgat aagttgcgtg ataaagaatt taaaaacttt gacgatttcc ggaagaaatt
     5161 ctgggaagaa gtgtcaaaag atcccgatct tagtaagcaa tttaaaggca gtaataagac
     5221 gaacattcaa aagggaaaag caccttttgc aaggaagaaa gaccaagtag gtggtaggga
     5281 acgctttgaa ttacatcatg ataaaccaat cagtcaggat ggtggtgtct atgatatgaa
     5341 taatatcaga gtgaccacac ctaagcgaca tattgatatt catcggggta agtaaaaatg
     5401 gaactgaaac atagtattag tgattatacc gaggctgaat ttctggagtt tgtaaaaaaa
     5461 atatgtagag ctgaaggtgc tactgaagag gatgacaata aattagtgag agagtttgag
     5521 cgattaactg agcacccaga tggttcagat ctgatttatt atcctcgcga tgacagggaa
     5581 gatagtcctg aagggattgt caaggaaatt aaagaatggc gagctgctaa cggtaagtca
     5641 ggatttaaac agggctgaaa tatgaatgcc ggttgtttat ggatgaatgg ctggcattct
     5701 ttcacaacaa ggagtcgtta tgaaaaaaat aacagggatt attttattgc ttcttgcagt
     5761 cattattctg tctgcatgtc aggcaaacta tatccgggat gttcagggcg ggaccgtatc
     5821 tccgtcatca acagctgaag tgaccggatt agcaacgcag taacccgaaa tcctctttga
     5881 caaaaacaaa gcgtgtcagg ctgattctga tgcgcttttt ttttgaaatg tcacaaaaat
     5941 tccatgtggg agatgggatc taaaatcccc gtgcagaact ttccatccag ggggagaaaa
     6001 cttgtcgttt tgagccgttc ggtgttcaga acgcacgaaa ccgatcgcgc atcgctttcg
     6061 tgcatagtta tgcaacgcgc taaaaacgat tctgacgcat tttttatgtt taccctggtg
     6121 tttccttgtc tttttgcgtt ttttgcgtca gaaagcgatt gagggcgttt taaggggggc
     6181 gtacaacggg agttatggta aatggatcgg gttttcggga aggatcgaca ggatttgccg
     6241 ttgggtgtag tgtaagcgac tgaaaaacaa acgccccgta aatcgtgctc tcaccgccaa
     6301 gattgatcac gaaattacag ggcgccgggt tccgcgtttc ccgatgggaa agcgcggtta
     6361 gttaaactgt gtaccgagag aaatcgtatc acatgagcgc cgtacttcaa cgcttcaggg
     6421 aaaaattacc gcacaaaccg tactgtacga acgatttcgc gtacggcgtt cgcattctgc
     6481 cgaaaaacat tgccattctt gcccgtttca tccagcagaa ccagccacat gcactgtact
     6541 ggcttccctt tgacgtggac cggatggggg catcaatcga ctggagcgac cggaattgtc
     6601 cggccccgaa catcaccgta aaaaatcccc gtaacgggca cgcgcatctg ctctacgcgc
     6661 tcgcccttcc tgtgagaact gcgccggatg catcggcttc ggcgctcaga tacgctgccg
     6721 ctattgag
//
