LOCUS       NZ_CP086263             7939 bp    DNA     circular CON 05-JAN-2024
DEFINITION  Escherichia coli strain SCPM-O-B-9427 plasmid pB-9427-4, complete
            sequence.
ACCESSION   NZ_CP086263
VERSION     NZ_CP086263.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22830670
            Assembly: GCF_020808965.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Kartsev,N., Kislichkina,A., Teymurazov,M., Kuzina,E., Kanashenko,M.,
            Skryabin,Y., Sizova,A., Bogun,A. and Svetoch,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-NOV-2021) Science Department, SRCAMB, Building 1,
            Obolensk, Moscow Region 142279, Russia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 53.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/05/2024 14:31:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,437
            CDSs (total)                      :: 5,312
            Genes (coding)                    :: 5,008
            CDSs (with protein)               :: 5,008
            Genes (RNA)                       :: 125
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 95
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 304
            CDSs (without protein)            :: 304
            Pseudo Genes (ambiguous residues) :: 0 of 304
            Pseudo Genes (frameshifted)       :: 124 of 304
            Pseudo Genes (incomplete)         :: 198 of 304
            Pseudo Genes (internal stop)      :: 62 of 304
            Pseudo Genes (multiple problems)  :: 69 of 304
            Pseudo Genes (short protein)      :: 2 of 304
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086263.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="SCPM-O-B-9427"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pB-9427-4"
                     /geo_loc_name="Russia: Saint-Petersburg"
                     /collection_date="2018-07-30"
     gene            complement(join(7328..7939,1..981))
                     /locus_tag="LMH79_RS26940"
                     /old_locus_tag="LMH79_26965"
     CDS             complement(join(7328..7939,1..981))
                     /locus_tag="LMH79_RS26940"
                     /old_locus_tag="LMH79_26965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842142.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0042626 - ATPase-coupled transmembrane transporter
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_001009226.1"
                     /translation="MNFIEKYIITFNKCNLFFIILLSTISSFLFVLFGYLIKTVIDNKD
                     SIGEINSLLIFIACFLSIRFLMPAGYSISEYLTQKTNIELSVKLREQVIDNIQNSHQEH
                     FLKKNKGELNKVIESMLSSASSLFYTICSDVVPLLIQMIGIIITICISVNTLIAIEFII
                     IMAIYIIFVIKMTQRRFPMMKSVALSSKFASGRMFDMMHMYPMDKAFHTTDKSRKRVIQ
                     AVNTHSEKQRKVNNEFFLFGISSAFLSVIFSSLIILSAYWMFLHGRASRGSIIMLATFL
                     FQVFLPLNRIGYLFRQIKMARTEIDLYCAEINDIKKTNYTDKQYLHVKDNICNIEIYNK
                     SFNHKLILTKGVVTFITGENGSGKTTIAKILSGNITTEENTIKINGIQQEKANSPFVNV
                     LYVPQDLDLMPGTIEENILHYSGINNLNTIKKLLHRFKFDKPLDYEIKGYGHNLSGGQK
                     QKLGVSLTSGKNVDLIIFDEPTKGFDSLGIKIISDYIKEENKEKHIIVISHDEQLINNI
                     PKAQIIDITKHEV"
                     /name="ABC transporter ATP-binding protein"
     gene            complement(1020..1703)
                     /locus_tag="LMH79_RS26945"
                     /old_locus_tag="LMH79_26970"
     CDS             complement(1020..1703)
                     /locus_tag="LMH79_RS26945"
                     /old_locus_tag="LMH79_26970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_228355625.1"
                     /translation="MNSSSYAKVHNVSSLEDIMSYHNDDVLLKFRKEWNVTPEEADDIF
                     NETKKFIWLASTCLTECYNIKVHEQLQIIDEMWHTFIQFTDAYTSFCEKYLGAYLHHYP
                     NTNDMLKNEIRHVNEHGITFQEYRFNEYKNQIEKIAFYLGHETVAKWYGDYAVRYSIKN
                     INTIRIPKESISSDSYIEKVKSITHLPAAEFVKIIMRKDVWNDNGSVCGCSGKGCGAGC
                     SCNSR"
                     /name="hypothetical protein"
     gene            complement(2266..2424)
                     /locus_tag="LMH79_RS27285"
     CDS             complement(2266..2424)
                     /locus_tag="LMH79_RS27285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            complement(3083..3274)
                     /locus_tag="LMH79_RS26950"
                     /old_locus_tag="LMH79_26975"
     CDS             complement(3083..3274)
                     /locus_tag="LMH79_RS26950"
                     /old_locus_tag="LMH79_26975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165988.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDSENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3339..3662
                     /locus_tag="LMH79_RS26955"
                     /old_locus_tag="LMH79_26980"
     CDS             3339..3662
                     /locus_tag="LMH79_RS26955"
                     /old_locus_tag="LMH79_26980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_228355627.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVLAALMAIGEELRRLRLAVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3871..>4302
                     /locus_tag="LMH79_RS27120"
                     /pseudo=""
     CDS             3871..>4302
                     /locus_tag="LMH79_RS27120"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            4340..4858
                     /locus_tag="LMH79_RS26965"
                     /old_locus_tag="LMH79_26990"
     CDS             4340..4858
                     /locus_tag="LMH79_RS26965"
                     /old_locus_tag="LMH79_26990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_001673203.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVLFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="MbeB family mobilization protein"
     gene            4865..5098
                     /locus_tag="LMH79_RS26970"
                     /old_locus_tag="LMH79_26995"
     CDS             4865..5098
                     /locus_tag="LMH79_RS26970"
                     /old_locus_tag="LMH79_26995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137439.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            5809..6048
                     /locus_tag="LMH79_RS26975"
                     /old_locus_tag="LMH79_27000"
     CDS             5809..6048
                     /locus_tag="LMH79_RS26975"
                     /old_locus_tag="LMH79_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000703021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 family protein"
                     /protein_id="WP_000703021.1"
                     /translation="MKISDIEKNIYKGLAEFISQKIRDEHDICLGQFESEELLDEILKR
                     VIPVIYNVAIDDAIKSVRNVGDRLEEELDMRKVV"
                     /name="DUF2164 family protein"
     gene            6045..6407
                     /locus_tag="LMH79_RS26980"
                     /old_locus_tag="LMH79_27005"
     CDS             6045..6407
                     /locus_tag="LMH79_RS26980"
                     /old_locus_tag="LMH79_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_001547178.1"
                     /translation="MTNFKSDLNLIRKEWYNAFYSGNTEMLDYLEADWFISTNGQKVIY
                     KKHQLRKIDMRPARILPSIERYEYDVVMREFKGIACVSGKAEIKQNDGNIFMAFIENWI
                     KVNGGWKMQFISYEKI"
                     /name="nuclear transport factor 2 family protein"
     gene            complement(6432..7325)
                     /locus_tag="LMH79_RS26985"
                     /old_locus_tag="LMH79_27010"
     CDS             complement(6432..7325)
                     /locus_tag="LMH79_RS26985"
                     /old_locus_tag="LMH79_27010"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="WP_001547179.1"
                     /translation="MNNLIKNEIIEKFKKYNFQKYPFVFTDVNYKNLINWNDLNKLLEK
                     DILHYPRVRMANDNFPEIRGYKGFIRYTYSQTGDRTPHINRHQLYKCLRDGATLIVDRC
                     QSFFESVDESRLWLSKELECTCSANLYAAFTATPSFGLHFDNHDVIAVQIEGIKKWKVY
                     NPTYSYPLEDERSFDYLPPNTSPDYEFDITPGQAIYLPAGYWHNVTTQSKHSLHISFTV
                     IRPRRLELFKTLFDELKNNPYMREPIEHGDSLSDKEKIKTIITNAINSFDIQFAENMLK
                     AQSRTYRYKKIKLEHI"
                     /name="cupin domain-containing protein"
     Typing          2926..3005
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="94.0"
                     /evalue="1.83e-34"
     Typing          complement(2353..2895)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.862"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          3653..5202
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.787"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgaagatat tgcttgtctg tgtaatttgt tttttttatg tcgtttattt cggcacagta
       61 taaatcaatt tctgttcttg ccatttttat ttgtctaaat agataaccaa tgcgatttaa
      121 tgggagaaaa acttggaata aaaaagtggc aagcataata atactaccac ggcttgctct
      181 accatgaaga aacatccagt atgctgatag gataatcaga ctactaaaaa tgactgaaag
      241 aaaagcggag ctgataccaa acaggaaaaa ttcattattt acttttcttt gtttttctga
      301 atgtgtattt acagcttgaa ttactcgttt acgactttta tccgtggtat ggaaagcttt
      361 atccatagga tacatgtgca tcatatcaaa cattcttcca gatgcgaatt tggagctaag
      421 tgcaactgat ttcatcattg gaaatctacg ttgtgtcatt tttatgacaa atattatata
      481 gattgccatt attattataa actcaatggc tattaatgta ttaacactta tacaaattgt
      541 tatgattatt ccaatcattt gtattaataa tggtactaca tcagaacata ttgtataaaa
      601 taatgatgat gcagaagaaa gcatgctttc tataacctta tttagttctc ctttattttt
      661 ctttaaaaaa tgctcttggt gagaattttg tatattatca ataacttgct ctctaagctt
      721 tacagataat tctatatttg ttttttgagt caagtattcc gatatgctat atccagcagg
      781 cataaggaat ctaattgata agaaacatgc tataaatatt aataatgagt ttatttctcc
      841 tattgaatct ttattgtcaa taactgtttt tattaaatat ccgaatagaa cgaaaagaaa
      901 actacttata gttgataata atataataaa aaacaaatta catttattaa atgtgatgat
      961 gtacttttca ataaaattca atttataatc cttaaagccc gtatattacg ggctttatgt
     1021 tatctagaat tacatgagca cccagcgcca catcctttac cgctgcaacc acaaacagaa
     1081 ccattatcat tccaaacatc ttttcgcatt attattttta caaattctgc ggctggaagg
     1141 tgagtgatac tctttacttt ttcgatgtaa gaatcactgg atatggattc ttttggaatt
     1201 cttatagtat taatgttctt tatgctgtat cttacagcat aatcaccata ccactttgcg
     1261 acagtttcat gacctaggta aaaagcgatt ttttcaattt ggtttttata ttcgttaaaa
     1321 cgatattctt ggaatgttat accatgctca ttaacatgcc ttatctcatt ttttagcatg
     1381 tcatttgtat ttggataatg gtggaggtaa gcaccaagat atttttcaca aaagctggta
     1441 taagcatctg taaattgaat aaacgtatgc cacatttcat ctataatttg taattgctcg
     1501 tgaactttta tattgtagca ttccgttagg catgttgagg ctagccagat gaatttcttt
     1561 gtttcattaa agatatcatc agcttcttct ggtgttacat tccattcttt acgaaatttt
     1621 agaagaacat catcattgtg ataactcatg atatcttcta atgagctaac attatgaact
     1681 tttgcataag aactgctgtt catcttatgt tgtagcataa cacccccaat tatatatttg
     1741 aatgtagatg agaatagtta tcatttatta tagtgttgtt aaatttgtgc ttgtgtcaat
     1801 atgcctaatt ctataaatga gatagtaatc aagtttttta atggttatag gatccattag
     1861 taatacaagg tttttgcagc cagtttttac gggtaggggc tgcaaggaaa acaaaatttt
     1921 cccactctaa gagaagagaa ttgagttggt tgaatgagtg gatgcatcta gggccgctaa
     1981 ttcaccgagt gtaaaggctg tacgaaatgt gttgcagtcc gataatagcc ccctccattg
     2041 acgttttaca catgtgttgt aggttccagg agtgggcgga tctcaaaaag atcaaaggat
     2101 cttcttgaga tccttttttt ctgcgcgtaa tctgctgcca gtaaacgaaa aaaccgcctg
     2161 gggaggcggt ttagtcgaag gttaagtcag ttggggactg cttaaccggg taactggctt
     2221 ggtggagcac agataccaaa tactgtcctt ctagtgtagc cgcagttagg ccaccacttc
     2281 aagaactctt aatatctcaa tccaccttgt ccagttacca gtggctgctg ccagtggcgc
     2341 tttgtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgagcc
     2461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     2521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     2581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     2641 tttatgatgc tcgtcagggg ggcggagcct atggaaaaac gccaacggcg cagccttttc
     2701 ctggttctcg ttttttgctc acatgtttct tttggcgtta tcccctgatt ctgtggataa
     2761 ccgcatctcc gcttttgagt gagcagacac cgctcgccgc agccgaacga ccgagcgtag
     2821 cgagtcagtg agcgaggaag cggaagagcg ccggaacgtg cattttctcc ttacgcatct
     2881 gtgcggcatt tcacatcgga catggtgcgc tttccataca attcgcactg atgccgcata
     2941 gttaagccag tatacactcc gctatcgcta cgtgactggt tcagggcttc gccccgaaac
     3001 cccctgacgc gccctgacgg gcttgtctgc tcccggcatc cgctcacaga caagctgtga
     3061 ccgtctccgg gagctgtatg tgtcagaggt tttcactgtc atccccgaag cgtgcgaggc
     3121 agctgcggta aagctcgtcg gcgtggtcgt gaagtgattc acaaatatcg gcctgttcat
     3181 ctgcgtccag ttcgttgagt ttctccagca gcgttaatgt ctggcttctg ataaaacggg
     3241 ccatgttaag ggcggttttt tcctgtttag tcactgatgc ctccgtgtaa gggggatttc
     3301 tgttttatgg ggtaatgata ccgatgaaac gcgagaggat gctcaccata cgggttactg
     3361 atgatgaaca tgcccggtta ctggaacgct gtgagggtaa acagctggcg gtatggatgc
     3421 ggcgggtctg cctgggtgag ccggttgcac gctccgggaa gctgccgaca ctggcaccgc
     3481 cgttactgcg tcagctggcc gccatcggga ataacctgaa tcagacagcg aggaaggtga
     3541 acagcgggca gtggtcttcc ggtgatcggg ttcaggtgtt ggctgcgctg atggccatcg
     3601 gggaggagct gcgtcggctg cgtctggccg tcagggaaca gggcgcgcgg gatgatagtt
     3661 aaatttcatg ccaggggaaa aggcggtggc agtggtccgg ttgattacct gctgggaagg
     3721 gagcgaaacc gggaaggggc aacggtgctt cagggtaatc cggaagaagt tcgggaactc
     3781 atcgatgcca cgccatttgc gaagaaatac acgtcaggtg ttctgtcgtt cgcggagaag
     3841 gagctgccgc cgggaggacg tgaaaaggtg atggcgagct ttgagcgtgt actgatgccc
     3901 ggtctcgaaa agaatcagta cagcatcctg tgggtggagc accaggacaa gggacggctt
     3961 gagctgaatt ttgtcattcc gaacatggag ctacagagcg gaaaacgcct ccagccgtac
     4021 tacgaccggg cagacaggcc gagaattgat gcctggcaga cgctggttaa tcaccattac
     4081 gggctgcatg accccaacgc cccggagaac agccggatac tgactctccc tgataacctg
     4141 ccggaaacga aacaggtgct tgcagagagc gtcacacgcg ggatagacgc tctttatcat
     4201 gtcggggaga taaaaggccg tcaggatgtg attcaggcgc tcacagaggc agggcttgaa
     4261 gtggtcaggg tgacgcgaag tagtatcagc attgcagacc cgaacggcgg gaagaatatc
     4321 aggctgaaag gagcatttta tgagcaatct tttacagacg ggcgcggagt tcgagaaaaa
     4381 gctgaaagag agagccgaat ctacagagac aatgctgaac gacgagttca gcaagctcga
     4441 aaaatctgta agcaaggctg tgacatcaaa cgagacgaaa atcaaagacg ctatagccct
     4501 gttcacagct tcgacagagg aatcactgaa aaaacaccgg gaaggggtga aagaggcgat
     4561 gatgcagcac aggaaggacg tgttaaagct ggcagggaat acgggcatga tgttactggg
     4621 gatggtcctt ttcctgttta ccgtgagtgg cgggacgctc tggtatcttg gcgggatgat
     4681 acaggcgaac ctggaagaaa tcaggataca ggaagaaaca ttgcagaaac tgaacgcgaa
     4741 gacatggggc gtggagtttg tgcaggacgg gagaaggaaa tttctggtta tcccgcaggg
     4801 gaaatcggca acggtgattc cctatcaggg gaaggactgg gtacaactga cggagtgaca
     4861 cagagtgacg gagctggaaa gacatttgct gaacgcctta gagcaactgc aacaggacta
     4921 tatgcagcgg ctgaacgaat gggagagcgc cttcgtggaa ttgcagaaaa tgttttcgct
     4981 tacgcaacgg gacaacgcga tgctgaacga gcgggtcatg cagttgagtc agcaggtgca
     5041 gcacttgagc gagcagacag aacgcttgag ccagttatac agcgagaaca ggagataagg
     5101 gatgaacggc tgatacagga acgcgaacat gagttatccc tggagcgtga gcgccagctg
     5161 gaaatacagg aacggacaca ggatggccct tcgctgggat ggtaatcaga gacaggctaa
     5221 aatgttgtca gcgcgtttcc ggtgcatttt ttgggataat ggttggttcg ggggcaccgg
     5281 gaacgcgctt cagtcagacc ttgctgaaaa cacaggaacg cgttaaaacg catacgggcg
     5341 cgattctggt gtgtcttttg ttgctgtgtt ttgcctctat tgccagatcg cgctcattcc
     5401 cctgaacttc tgttttcccg ctccggtccc cgtcttcttc gcttccgaaa gcatgaaaaa
     5461 aatccgtttc agctgtctct ggctggcaat tcctgcgtga ttcacatggc tgcatagcta
     5521 tgcatgtggt taaaatttac cgggtgcgat cgcggcagtt tttcgggtgg tttgttgcca
     5581 tttttacctg tctgctgccg tgatcgcacc gaacgcgttt cagcggtgcg tacaataagg
     5641 gattatggta aattatcttg ttccttagag tatgattcga ggtggtatcc tgcacttatt
     5701 taaatatgct tgcttgggaa attccaggtt gcagtatggt tgagttgtaa ttttcctatg
     5761 ataggaaaaa tgttatatta ttaatttatt tgtgaggctt cataaagaat gaaaatttct
     5821 gatattgaaa aaaacatata taaaggacta gctgaattta tttcacaaaa aatcagagat
     5881 gaacatgata tatgccttgg acagtttgaa tcagaggaat tacttgatga gattcttaaa
     5941 agagtaatcc cagtcatata taatgtggct attgatgatg ctataaaatc agtaaggaat
     6001 gttggggata gattagagga ggaattagat atgaggaagg tagtgtgact aattttaaat
     6061 cagacttaaa tctaataaga aaagaatggt ataatgcatt ttattcaggt aatactgaaa
     6121 tgctagatta cttggaagct gattggttta tttcaacaaa tggtcaaaaa gttatctata
     6181 aaaaacatca gttacgaaag attgatatgc gtccagcgag gattttacct agcatagaaa
     6241 gatatgaata tgatgtcgta atgcgtgagt ttaaaggcat tgcttgtgtt tcagggaaag
     6301 ctgaaataaa gcaaaatgat ggaaatatat ttatggcatt tattgagaat tggataaagg
     6361 ttaatggtgg atggaaaatg cagtttatat cgtatgaaaa aatctaaggg gaagccccct
     6421 tagattaatg gttatatatg ttcaagtttt atttttttat agcgataagt tctgctttga
     6481 gctttaagca tgttttctgc aaactgaata tcaaaactat ttatagcatt agttattata
     6541 gtttttattt tttctttatc agataatgaa tcaccatgct ctataggttc ccgcatatat
     6601 ggattgtttt ttaattcatc aaataatgtt ttaaatagtt ctaatcgacg aggtcttata
     6661 actgtaaaag atatatgaag agagtgtttg ctctgagtcg taacattgtg ccagtatcca
     6721 gcaggaagat atatggcctg acctggggtt atatcaaact cataatctgg ggaggtatta
     6781 ggtggtagat aatcgaagct tctttcatct tcgagagggt atgaataggt agggttgtaa
     6841 actttccatt tttttattcc ctcaatttga acagctatta catcatgatt gtcaaaatga
     6901 agcccaaagc ttggtgttgc tgtaaatgca gcatatagat tagcactaca tgtacattct
     6961 aactctttag ataaccatag tctgctttca tctacagatt caaagaatga ctggcatcga
     7021 tctactataa gggttgcgcc atcacgtaag catttatata gctgatggcg atttatatgt
     7081 ggtgttctat cgcctgtttg gctatatgtg tatcttataa atcctttata ccccctaatt
     7141 tcgggaaagt tgtcatttgc cattctaact ctaggatagt gcaatatatc tttttcaagc
     7201 aatttattaa gatcattcca gttgattaag tttttataat taacatccgt gaatacaaac
     7261 ggatatttct ggaaattata tttcttaaat ttttctatga tttcattttt tataagatta
     7321 ttcattttta aacctcatgt tttgtgatat ctataatctg tgctttaggt atattattta
     7381 taagttgttc atcatgagat ataacaatga tatgcttttc cttgttttcc tcttttatat
     7441 aatcactgat tatttttata ccaagtgaat caaacccttt tgtcggttca tcgaaaataa
     7501 tcaagtctac gttttttcca gatgtaagag atactcctaa tttttgtttt tgtccaccag
     7561 ataaattatg tccgtaacct ttgatctcat aatctagtgg tttgtcgaac ttaaatctgt
     7621 gtagcagctt tttaattgta tttaaattat taatgccgga ataatgtaat atattttcct
     7681 ctatagttcc aggcatgaga tctagatctt gaggaacgta taagacatta acaaaaggag
     7741 aatttgcttt ttcttgttgt atcccattga tttttattgt gttttcttct gttgttatat
     7801 tgccagagag aatttttgca atagttgttt ttcccgaacc attctcaccg gttataaagg
     7861 taacaacgcc ttttgttaat attaatttat ggttgaatga tttgttgtat atttcaatat
     7921 tgcaaatatt gtcttttac
//
