LOCUS       NZ_CP086227             4018 bp    DNA     circular CON 21-MAY-2024
DEFINITION  Escherichia coli strain 779 plasmid p779-7, complete sequence.
ACCESSION   NZ_CP086227
VERSION     NZ_CP086227.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22826253
            Assembly: GCF_023024425.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Bao,D., Xu,X., Wang,Y. and Zhu,F.
  TITLE     Emergence of a Multidrug-Resistant Escherichia coli Co-Carrying a
            New mcr-1.33 Variant and blaNDM-5 Genes Recovered from a Urinary
            Tract Infection
  JOURNAL   Infect Drug Resist 15, 1499-1503 (2022)
   PUBMED   35411158
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Bao,D.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-NOV-2021) Department of Clinical Laboratory, Sanmen
            People's Hospital, Taihe Road, Taizhou, Zhejiang 317100, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_023024425.1-RS_2024_05_21
            Annotation Date                   :: 05/21/2024 03:58:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,658
            CDSs (total)                      :: 5,531
            Genes (coding)                    :: 5,280
            CDSs (with protein)               :: 5,280
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 96
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 251
            CDSs (without protein)            :: 251
            Pseudo Genes (ambiguous residues) :: 0 of 251
            Pseudo Genes (frameshifted)       :: 87 of 251
            Pseudo Genes (incomplete)         :: 172 of 251
            Pseudo Genes (internal stop)      :: 48 of 251
            Pseudo Genes (multiple problems)  :: 49 of 251
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP086227.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="779"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="p779-7"
                     /geo_loc_name="China: Zhejiang"
                     /collection_date="2016-11-03"
                     /collected_by="Danni Bao"
     gene            complement(151..678)
                     /locus_tag="LMB17_RS28145"
                     /old_locus_tag="LMB17_28150"
     CDS             complement(151..678)
                     /locus_tag="LMB17_RS28145"
                     /old_locus_tag="LMB17_28150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            complement(807..1022)
                     /locus_tag="LMB17_RS28150"
                     /old_locus_tag="LMB17_28155"
     CDS             complement(807..1022)
                     /locus_tag="LMB17_RS28150"
                     /old_locus_tag="LMB17_28155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1026..1511)
                     /locus_tag="LMB17_RS28155"
                     /old_locus_tag="LMB17_28160"
     CDS             complement(1026..1511)
                     /locus_tag="LMB17_RS28155"
                     /old_locus_tag="LMB17_28160"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<1546..2250)
                     /locus_tag="LMB17_RS28390"
                     /old_locus_tag="LMB17_28165"
                     /pseudo=""
     CDS             complement(<1546..2250)
                     /locus_tag="LMB17_RS28390"
                     /old_locus_tag="LMB17_28165"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2189..2512)
                     /locus_tag="LMB17_RS28165"
                     /old_locus_tag="LMB17_28170"
     CDS             complement(2189..2512)
                     /locus_tag="LMB17_RS28165"
                     /old_locus_tag="LMB17_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            3160..3273
                     /locus_tag="LMB17_RS28170"
                     /old_locus_tag="LMB17_28175"
     CDS             3160..3273
                     /locus_tag="LMB17_RS28170"
                     /old_locus_tag="LMB17_28175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            complement(3702..3914)
                     /locus_tag="LMB17_RS28175"
                     /old_locus_tag="LMB17_28180"
     CDS             complement(3702..3914)
                     /locus_tag="LMB17_RS28175"
                     /old_locus_tag="LMB17_28180"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_247150873.1"
                     /translation="MGDFERTFGAGADFERIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     Typing          complement(2626..2803)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          2750..3273
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.434"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          complement(716..2199)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caccgttctg cgccatgaac cgcaaaagag cacctcttac cacgtaaaaa cgccgataaa
       61 tgccccccgt aagttcttac ggtatgcata gttatgcacg gaaatgaact gacactctgt
      121 tcaatggatt ttcatgctgt aagcagtatt ttagtcttct atgccccctc gaaacacacc
      181 tgttcttgtg tagatctttt cgacccaaga tggtctttta tccgagttca tgcagccgta
      241 caggaacaca cctgttttgg gtttatagct ccccccctcc tgtggctggt tatctacaaa
      301 tgaataatca atcttttcac ctttgactga agcacgcccc ccaacatgaa ctaaagacca
      361 gtgctcatgc atcacatggc tttcaatcaa tccaaatgaa gaaaaacccc atgaatcaaa
      421 aaagaccttg agtccgtcag acgcaccaac aggagtaatg attgcaaatt caacctgctc
      481 accagtacct tcaaggtaag aaggtttctc gacgttaatt acagatgccc ccttcacctg
      541 ataaccttta caggtaagat ataagtccct cccggctgac ggttttgtat atgtaacgtt
      601 gtcgttattc tccgcagcag cgactgtaaa tgacatagcc aaaaccaccg agactgagca
      661 agcccagaaa catttcataa cgcatccctt ttcattgatt aactacattc caagctcaaa
      721 acctttctgc cgctgcgatc gttccagctc cagcgctttc tggtgctgcg ccgcgcgctc
      781 ctgctcccgg tgcgcctcga tgccattcag ccttgcgaca atcggcgcag ctgatcgctc
      841 aaactctgca cctgcctgct caagcccgtg acgcgctcgc tcagcgccgc gttctcccgt
      901 tgcataagcc cagacatcgt ccgccattcc gcgaaggcgt tctcccactc gtccagcctt
      961 ttcgagtagt cctgctgtag ctgctctaat gcgctcagca actgtttttc cagttccgtc
     1021 atgtgctatt tccccgccag tatcatccag ctcgaatcct ctccgaatct tcccgcttcc
     1081 gggttcaccc tcacgcagcg gcgtctctcc tccccgcagg tcgtgagctg gacgccgttg
     1141 ttcttctctg aaagcatggc ctgcgtgctc ttctgttcct ggatggtcgt gtagttgtcg
     1201 agtatctgtt gcccctgcca ccacagaatg cccgcgctcg aggcgatcag tagcgcggag
     1261 accaggacta tggtcagcca cgtctggctg atcatacgca gcatcccttt cgtgcgttgg
     1321 ctcatggctg aggacagctt ccggtcgtgg tcgaggatgg cggcgctgat tctcttctcg
     1381 ctttcgctca gttccgctct gacagacttc tcgtgctcgc tgaatgcggc tttcagcatc
     1441 tcgccggtag tctgctgctg cgctttcgat ttctgctcta agtccttcgc cagcgttaaa
     1501 agactgttca tagatggctc ccttcagtcg gatgtttcgc cccccgtcag ggtcggcaat
     1561 gctgatgctg cttttcgtgg tgcgcaccac ctcaaaaccc gcactttcca gcgcctcagt
     1621 gacgtcctga cgggttttca gctccccgga tgaggcaagg gaaagtaggc cgcgcgtaat
     1681 cgcctgggcg gcttcctgct tcgcttccgg cagcgcggac ggtgtaacca gcgcccgccg
     1741 gttttccggc gcgttcgggt cgtgcagccc cagtctgccg ttcactatgg tctgccaggc
     1801 atcaatgcgc ggacggtcgg cgcggtcgta atacggctgg agacgtctgc cggtcagcag
     1861 ctccgtgttc gggatcagga agttcagctc cagccgccct ttgtcccggt gctcaaccca
     1921 cagtacgctg tactggtctt tatcgagtcc gggcatcaga acccgctcaa agctcgccat
     1981 cagcttttca cgctggccgg gcggcaaatc ctgttcggca aaagacagca cgccggaggt
     2041 gtatttcttg gcgtaaggcg aggcgtcaat aagctcccgc acttcctccg gcttaccctg
     2101 cagaacgctg gcgccgtcgc gctgccggtc tttccccagc agataatcca ccggaccgcc
     2161 gccaccgccg cggcctctgg gatgaaactt aacgatcatc gtctgccccc ttttccagca
     2221 cggcgtgccg cagccgctcg agtccggcat caatggccat cagcacggcg acaacctgca
     2281 cgcgatcgtg cccagtaccg ccaccggcgt taacctgccg ggcaatctgg ttgaggttgt
     2341 tacccatgcc ggcaagctga cgaagcagcg ccggcgagat ggatggaagt ttgccagcac
     2401 gcgagggctt ttcgtccagg catatctggc gcatccaggc cgcgagttgt ttaccctcac
     2461 agcgctccag aagtcggcgg tgctcgtctt ctgtcaccca catagtgagc attttgttgc
     2521 gcttttctgc caaaccatcc tccggataac accggttcgg gcgggaaatt ttttcctgtc
     2581 cggagcggtg ttgggcaagc ccgcagggcg cggcagtact tttcagcggg tttcggggcg
     2641 cagccctgaa ccagtcacgt agcgctagcg gagtgtatac tggcttaaca tggtaaatcg
     2701 gagtggggat tcaggaaagt gcttcatgtg gcagggagaa aatgctgctg tggggcgtaa
     2761 gtgcgaaatg cagatcccgg tgaaatgacg cctcctcgct cactcggtcg ctgcgctcct
     2821 gccgtgagac tgcggcgggc gttgccagct tacgaatggc tggataaaga gactaagcgg
     2881 aaatcaatca gaaagggcgg aaaaaggatg cggcgttgcc gtttttccat aggctccgcc
     2941 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gtggcgaaac ccgacaggac
     3001 tataaagatc ccaggcgttt ccccctggta gctccctcgt gcgctctcct gttcctgcct
     3061 ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt atctaattcc acgcctgaca
     3121 ctcagttccg ggtaggcagt tcgctccaag ctggactgta tgcacgaacc ccccgttcag
     3181 tccgaccgct gcgccttatc cggtaactat cgacttgagt ccaacccgga aagacacgac
     3241 aaaacgccac tggcagcagc cactggtaac tgatttagag gagttagtct tgaagttatg
     3301 cgccggttaa ggctaaactg aaaggacaag ttttggtgac tgtgctcctc caagccagtt
     3361 acctcggttc aaagagttgc tgctctgaga accttcgaaa aaaccgcctt gctgggcggt
     3421 tttttcgttt acagtgctaa gagattacga ttctccatga atgatctcaa gaagatccgt
     3481 ttaaaaaagc gggttttatg gggggataaa gttcgtctag cccagtcact ttggattaca
     3541 gctaggtttc gtaagccatg caagaatgcc tgtaactaac aaaataggcc ctgcaacgta
     3601 cgtaacatag actccaacat acgacactgt gaaacatatg agtacgtata tagacagtgt
     3661 tgtccaaaaa atgactgttg ttttacagtg aatccactct ttcatttttc aataaacccc
     3721 acaccgtctg gagatcttat tatcgttttg ccaggattag ctttcgccca aaccaaagca
     3781 tcttgaaaag aagagaattt tttttttgaa tttttagttt ttcggcgatc ttctcgaaaa
     3841 tattcgctgt tattctcatc aattatccgc tcaaaatcag caccagctcc aaaggtacgc
     3901 tcaaaatccc ccattttctg cccccattaa atttaataaa aaaacagatt taaaatatcc
     3961 aaaagacact cttatttacc ataacatccc ttgttcgcac cgccgcagac agcctcag
//
