LOCUS       NZ_CP085916             2056 bp    DNA     circular CON 24-DEC-2023
DEFINITION  Enterococcus faecium strain SC1762-D plasmid p10, complete sequence.
ACCESSION   NZ_CP085916
VERSION     NZ_CP085916.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22627489
            Assembly: GCF_020736625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Zeng,W.
  TITLE     Acquisition of daptomycin resistance by Enterococcus faecium confers
            collateral sensitivity to glycopeptides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Zeng,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) Laboratory Medicine, College of Life
            Sciences, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 20:46:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,310
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,060
            CDSs (with protein)               :: 3,060
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 63 of 159
            Pseudo Genes (incomplete)         :: 94 of 159
            Pseudo Genes (internal stop)      :: 41 of 159
            Pseudo Genes (multiple problems)  :: 30 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085916.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="SC1762-D"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p10"
                     /geo_loc_name="China:wenzhou"
                     /collection_date="2018"
     gene            join(1970..2056,1..171)
                     /locus_tag="LLE91_RS16475"
                     /old_locus_tag="LLE91_16475"
     CDS             join(1970..2056,1..171)
                     /locus_tag="LLE91_RS16475"
                     /old_locus_tag="LLE91_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002377879.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(307..909)
                     /locus_tag="LLE91_RS16480"
                     /old_locus_tag="LLE91_16480"
     CDS             complement(307..909)
                     /locus_tag="LLE91_RS16480"
                     /old_locus_tag="LLE91_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_010729575.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(976..1131)
                     /locus_tag="LLE91_RS16485"
                     /old_locus_tag="LLE91_16485"
     CDS             complement(976..1131)
                     /locus_tag="LLE91_RS16485"
                     /old_locus_tag="LLE91_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            1748..1969
                     /locus_tag="LLE91_RS16490"
                     /old_locus_tag="LLE91_16490"
     CDS             1748..1969
                     /locus_tag="LLE91_RS16490"
                     /old_locus_tag="LLE91_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          74..249
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          complement(380..909)
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 aaagtcgaag aaaccaccga cctctttgac ctacttctca caaatattct ggaattaaac
       61 tctgaagaac aaaaagagca taagggaaaa ctgatagcac cttcctacag gaaggcggct
      121 aagtcggctg tgccaactgg ttttcttaca aaacaacctt atattttttg atatcacaaa
      181 aaaatccatt ttggacctat tttcggtcat aatatagtac ctacttttgg tcatagtttc
      241 gtccgtagtt ttggtcatag tgactaagac tttcacgcac aaaaaaagag ctgaaattaa
      301 cagctcttat cctttattta tttttgaatt tacacttcca ccatgacgat tcgattttat
      361 atattgcccg ataatataag ctgaattatc acaaagaagc ggaaaccaat catcaaatct
      421 ttctgacatt gcatagtcaa ttaaatcttt gatttcagtg atattcttag aatcaacaaa
      481 cgacatcatc tctctaatca actcatatcg ttctgcactc gttactgaca aataactagc
      541 aatttctgca ccaccatgag cgataatatc acttttagca tattgaaatt tttctggatt
      601 atccatatga gcaaaatatc gcaccatacc ttttgcatta gcacattttt gcgggtttgg
      661 agaccgaatc ttttgagtta tctcacgtat ttgatcatac gtctttttac cgctaaacat
      721 tatcaaaaca tgccaatgag gctttttcaa ttcaccgtca ggattcacat ctttatcatg
      781 aagtggactc tcaacccaag gaacatgtaa ctcatctaaa aactccctcc aattttcagg
      841 agccgattca ggatagacta caaacgtcca attccttgtg cgttcatctt tagtttttct
      901 actcgtcatg ctataatcac cttaattgaa caacagtcta acagcaaaag cagtgcgcca
      961 acactaatgc ttttgctatt ttttttgctc attttccttt ccgttagcat taacccaaat
     1021 tgtaataaga ccagacttac taagacccat ttgtttcgca attttatcaa gattatctgc
     1081 cacttcctta gtaagcgtta caacgactct ctttttttca ttactgatca taattcgtat
     1141 cctccaacga ctttttctta aaaaccatag taacaaaaaa gtaactaagg tgcaacagtt
     1201 tagatactaa ttagcgaata ataggcctta aacccttggc attactgatt ttgtcaaaaa
     1261 aaaacgctga aacccttggg agagtaaggc taaacagcta tcgaccaatg accaatgacc
     1321 aataaggggg gtgtcgtagt ccccccctcc tccgcccaat tcataaacac caaaaccaaa
     1381 aaacaaacct cagggctccg ccccgaaccc cgacctcctt ctaccgaaat atatgcaggc
     1441 gccacgctta ctatttcgtt cagctatcgg cttttgcaat ctaacacgac cgtttcgcta
     1501 ccagtcgcct tacaggctcc attctcgctt ctcttacgtg ttttaacgat tgcgccgata
     1561 actaaacgaa atagacgcta aatcgcttaa aacgcaaaat agaccctttt tagagcattg
     1621 attttttcta ctagtcattt cgataatata aaattgacca caacacagaa aacttatatt
     1681 ataagcttat gtcgtaagtc cgacgtactc acgtaatata gacggacgtt aaatggaagg
     1741 agcataaatg agagtgataa aatcagaaaa attcactttg gccgaaatta tgacgaccag
     1801 cgaacttgca caagaacttg attacaacca agcttacgtt ttgcgcttag cgaaagaaca
     1861 tctgactgaa ggaaaagaat atcgctctgc cggtagaaga aactatcttt tcagcccaga
     1921 agcccttaaa aaactagaaa aagtcataac caccaaagaa ggtgagtaaa tgcctaaaga
     1981 aacaactatc aacgttgaac aactagaaaa attgctagaa tcatattcca atgattttat
     2041 cgaattgacc ggcgga
//
