LOCUS       NZ_CP085911             7838 bp    DNA     circular CON 24-DEC-2023
DEFINITION  Enterococcus faecium strain SC1762-D plasmid p5, complete sequence.
ACCESSION   NZ_CP085911
VERSION     NZ_CP085911.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22627489
            Assembly: GCF_020736625.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Zeng,W.
  TITLE     Acquisition of daptomycin resistance by Enterococcus faecium confers
            collateral sensitivity to glycopeptides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Zeng,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) Laboratory Medicine, College of Life
            Sciences, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 120.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 20:46:10
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,310
            CDSs (total)                      :: 3,219
            Genes (coding)                    :: 3,060
            CDSs (with protein)               :: 3,060
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 159
            CDSs (without protein)            :: 159
            Pseudo Genes (ambiguous residues) :: 0 of 159
            Pseudo Genes (frameshifted)       :: 63 of 159
            Pseudo Genes (incomplete)         :: 94 of 159
            Pseudo Genes (internal stop)      :: 41 of 159
            Pseudo Genes (multiple problems)  :: 30 of 159
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085911.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="SC1762-D"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p5"
                     /geo_loc_name="China:wenzhou"
                     /collection_date="2018"
     gene            join(7798..7838,1..403)
                     /locus_tag="LLE91_RS16275"
                     /old_locus_tag="LLE91_16275"
     CDS             join(7798..7838,1..403)
                     /locus_tag="LLE91_RS16275"
                     /old_locus_tag="LLE91_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            1574..2542
                     /locus_tag="LLE91_RS16280"
                     /old_locus_tag="LLE91_16280"
     CDS             1574..2542
                     /locus_tag="LLE91_RS16280"
                     /old_locus_tag="LLE91_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            2559..3083
                     /locus_tag="LLE91_RS16285"
                     /old_locus_tag="LLE91_16285"
     CDS             2559..3083
                     /locus_tag="LLE91_RS16285"
                     /old_locus_tag="LLE91_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_044383356.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            3259..3663
                     /gene="tnpA"
                     /locus_tag="LLE91_RS16290"
                     /old_locus_tag="LLE91_16290"
     CDS             3259..3663
                     /gene="tnpA"
                     /locus_tag="LLE91_RS16290"
                     /old_locus_tag="LLE91_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            3680..4828
                     /gene="tnpB"
                     /locus_tag="LLE91_RS16295"
                     /old_locus_tag="LLE91_16295"
     CDS             3680..4828
                     /gene="tnpB"
                     /locus_tag="LLE91_RS16295"
                     /old_locus_tag="LLE91_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            5350..5631
                     /locus_tag="LLE91_RS16300"
                     /old_locus_tag="LLE91_16300"
     CDS             5350..5631
                     /locus_tag="LLE91_RS16300"
                     /old_locus_tag="LLE91_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            5633..6049
                     /locus_tag="LLE91_RS16305"
                     /old_locus_tag="LLE91_16305"
     CDS             5633..6049
                     /locus_tag="LLE91_RS16305"
                     /old_locus_tag="LLE91_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            6725..7825
                     /locus_tag="LLE91_RS16310"
                     /old_locus_tag="LLE91_16310"
     CDS             6725..7825
                     /locus_tag="LLE91_RS16310"
                     /old_locus_tag="LLE91_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain"
                     /protein_id="WP_224198038.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="cysteine protease staphopain"
     Typing          1575..2538
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttagttatg ggtctagtgt tagttctttt cttaactagt tgttcaaaaa aacaagaaat
       61 acaaaagact gatctgattc cttttctagg aacttggcaa actaaagatc attcagaacg
      121 tctagtaatc actaatttaa atacttcaaa aaatatagaa caatatacca tcttagtaaa
      181 agagaacaat cataaaattc aaatcattga taaaaattta aatataaatg aacaagtttt
      241 catttacaaa gcaacaaata atttactaac actgcgctct aataaaaaac aattagatta
      301 cacttttagt ttggattctc caaaaaagct cgtatttatt ttgacaacga atgatgggac
      361 agaagggaca gctaaaccaa ttgaattttt taaagctgat tgatgaatag ttgtttgata
      421 ttacaaaaca ataatttcac cgaaggtgaa acaagcgaaa ctttacaaaa agtggctagc
      481 atttgctagc cttttttgta ttgtggagcg tgggttttga gggggattca agggggagct
      541 tgcgcccctt tgaaaagcgg ttatttgcac caaatacgta ggtatttggc tagcaaatat
      601 agagcttgcc aaacacttta gtgagttttg tagaaagtac ctttaaaggt actttcacat
      661 tacgaacgat acgtccgtag gacgtactga aagggtggga tttatgagcg aactaatgca
      721 aaatttggct agcaaaaaaa gttaaaaaat atatgctaat aatcgagcga aatgaacact
      781 ttttcaagtg agcgaatccg ccatgacaaa gctgaagcag tttatttcag ctttgtcatg
      841 gcgtgtgcct agacttttaa aaaagtcggc acacgagcaa atatctttat caagctctaa
      901 tttgcgtttt aagacgtttg aaacgtctaa gtatataaat aatataagag taggtttaag
      961 agtgcttaaa tcgaatttta gagggcattc agacaacttt agggttgtta gggcttgccc
     1021 tgaccgcctg taaggcgctt taagaacaag ataagggatt tatactaaga ctgtcttaaa
     1081 acgtctcaaa cgaaggctca tagcttctca aagagttcct tatgaacatt tttagcaaag
     1141 gagccggaag cgactgcgca gtataatgtg agagcagagc gatcacacct ttttaagcga
     1201 ctgtagggag taagattccc agttgagaaa aatgcgcgaa tctgtttgga gaaaaaagaa
     1261 gtggtttttt aggcgggaaa ttttgtcccc ctcttttata atctcttttt aaacctcttt
     1321 ttaaacctct ttaccgtcgg ctatatcctt actctcccaa gggaatgacg cttttatcta
     1381 catcgttttg tctgtttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt
     1441 tatctacatc gttttgtctg tttgtacatt gaattagtac atcgacgatg ttaatataag
     1501 atgtagataa acaggcattt tgtatttcat aacgtgtatc tcttgtgcga gtaagaaagg
     1561 agctatttta aatatgtcta atgaaatcgt aaagtatcat catgaattaa atacaattcc
     1621 gttgcgaaaa tttacaccag tcgaaatgaa tttgttcttt tctatcgtat caagaatgcg
     1681 tgatgtaggt gataaaaaag tacaatttac attcgagcaa ttaaaagatt taagcaatta
     1741 taaagcaact gcaaatgttc gattcataga tgatttagaa acaacgtatg acaaattaat
     1801 ggatttacgt tttggtagaa gaagtgctga cggtttgcaa cgtgaacgtt ttgttttatt
     1861 taatcaattt aaaatcgacg gaaaatctga tattcctttt gctgaaatac aagttcatga
     1921 aaaagcctta cccttactta ataatcttga agaatgggtg cgctattctc tccaacaatt
     1981 caatgaatta gaaagcagtt attcaaaaac aatgtttcga ttactaaaac aatacagaac
     2041 caaaggtttc gcttattttt caaaagaaga ctttcatgaa ttacttgata ttcctaaaag
     2101 ttacaaccaa ggtacgattg atcaacgtgt gttcaatcca atacgacaag aactaaccgc
     2161 aatctttaaa ggtttaacaa tcaaaaagaa atacggaaaa ggacggggaa agcctgtcat
     2221 tggctatcaa tttacattta aacctgagat aaaaaaatca gacgacttta acaaacatgc
     2281 cccaacaaaa gaagaacaaa acaaagcatt agaactagct aaccaacaag ttcaatctga
     2341 aaaagcacct acttcattac aagaaaaaat caaagaacaa aagcgaaaat tggaactttt
     2401 acaacagtta gaaaagttag aaagagaaca aaatacccaa aataaagaaa ataatgcaca
     2461 aaaatcagag ataaattccg atattccttc agaactaaaa aaagacttat tagacaatct
     2521 tcaaaactta ttcaaagatt gaggggtttt tagattttat gacagaaaaa acaatcaaag
     2581 aacttgctga agaattagga gttagtaaaa cagcaattaa caaaaaagtt tctgacgaaa
     2641 atcgaaaact ttggttttcg aaaatcggaa acaagtttgt gattaatgaa accggacaaa
     2701 aggctataaa atcaatgttt tttaacgaaa acgaaaactc aaatcaacaa ctagttggcg
     2761 aaaaaacgaa aaccgaaaac cacaaaaacg ccaaccaagt tcccgaatta gaagtccttt
     2821 ctctcctaga acaacagtta aatcaaaaag atatgcaact acaagaaaaa gacgagcaaa
     2881 taaaacaaat gcaaaaatta ctcgaccacc aacaacaact caccctgcaa gccaaccaac
     2941 aaatcgagaa actgcaggaa caactacagt tgacttatac ggaagaatct tcggaaaata
     3001 aatcaacgac tctttctgaa gaaacagaat cgatagaaaa acaatcaaag gcggagaaaa
     3061 aatggtggca attctggcaa taaaaccagc aatttaacag aattaggcag aagtcgccca
     3121 tcctctatag gtgggtgatg aatgccgttc ggtatgaggg ttaccgttgg taactcgaag
     3181 accgacaact aaggcgtatt gcctttttat tttgtcccat gttacaatca gtctatacaa
     3241 gaaatgagtt gatataacat ggaattagat acaaataatc attcagtatt tcttctttat
     3301 taccaccttg ttttagtaac gaaatatcgt agacaagtga ttgatgatga aatatctgat
     3361 tatgccaaaa ctacttttga aagaatttca gaatcgtatc acattacttt agtggagtgg
     3421 aatcatgata aagaccacgt tcatattatg ttcaaagctc aacctaaaac agaattgaca
     3481 aaattcatca atgcctataa aagtgccagc tcaagattga taaaacgtga ctttccaaga
     3541 gtcaaacaat ttctttggaa agaaatgttc tggtctaaaa gtttttgtct tctgacaact
     3601 ggtggcgcac caattgatgt gattaagaag tatatccaaa atcaaggaaa caatcataaa
     3661 tagaaagtag gtgaacttta tggaacaact aaaagcatac aaattcagaa tctatccaac
     3721 agaggaacaa gaaatcttct ttgccaaatc ttttggttgt gtccgtaagg tctacaatct
     3781 aatgcttgat gaccgaaaaa aggcgtatga agaagttaaa aatgattctt ctaaaaaaat
     3841 gacttttccg acaccagcta aatacaagaa agaatttccg tttttgaaag aaattgatag
     3901 ccttgcttta gccaatgccc aacttaattt agataaagca tacaagaatt tttttcggga
     3961 taaatccgtt ggatttccac gtttcaaaag taagaaaaat cctgttcaga gttatacaac
     4021 gaataatcaa aatggtacag ttgctttgat tgatagtaaa ttcatcaaag ttcctaaatt
     4081 gaagtcatta gtaagaatta agcttcatag acaaccaaaa gggattatca aatctgctac
     4141 aatatcacgt cattctagcg gtaaatacta tatttctttg ttgtgtaaag aagaaattat
     4201 cgaattacct aaaactaatt ccacaattgg tattgactta ggtattatgg actttgcgat
     4261 tctttctgat ggacaaaaaa ttgataataa taaattcacg tccaaaatgg aaaagaaact
     4321 aaagcgtgaa caacgcaaat tgtctaggcg tgctttattg gctaaaaata aaggtaccaa
     4381 tctatttgaa gctaaaaatt atcaaaaaca aaaacgcaaa gtagctagat tgcatgaaaa
     4441 agtaatgaat caacgtactg attttctgaa taagttgagt acagaaatga tcaaaaatca
     4501 cgatattatc tgtattgaag acttaaacac aaaaggtatg ttgcgaaatc ataaattagc
     4561 taaaagtatt tctgatgttt cttggtcaaa gtttgtgact aaattacaat acaaggctga
     4621 ctggtatgga cgaaaaatca tcaaagtaga taaatggttt ccgtctagtc agatttgttc
     4681 agagtgcgga cataaagatg gcaagaaatc tcttgaaatt cgagaatgga cttgtcctgt
     4741 ttgtcatact catcatgatc gtgatatcaa tgctagtatc aatattctga ccgaaggtct
     4801 aagactacat tctatgggat tggcttagat ccaatcatca agaaccgtag gaactacggg
     4861 gatagcttgg taaataagag acaccgctgt tagtaaagaa atacgctatc aagtatgctc
     4921 tattcccaag aagctcccac ttcaagcgtt aagaaccgtc aggtttagct aagtggtgag
     4981 tagttcacat taataaaaat tgctggtttt attcttcttt ttgcttttgc gagcagagca
     5041 acatggaccc atgttgcgat attttggtgg cgccccaatc cccgtttttt cgttgaatct
     5101 tactcggact gcactcgttt tacagctgat ttttctgaat tttgaaagag cataaggaaa
     5161 cgtggtatca ctttcttacg aaaggtgcta gataggctgt gcctatcacg tttctcagaa
     5221 aaaaatttgc tatcactttt ttgccattgt ggctaggcta tgtatagtgt tttggcgaaa
     5281 aatagctata caaatactat atttgtatgg tatacataaa ttatacgctg atacgaaagg
     5341 aaaatttcca tggacatttt agttcgaaat atcaatccca aaattattgc aagaatagat
     5401 aaacgttgtc aggaactcac aaccaaaacg ggcaaaaaat ggagtcgaaa taaatatcta
     5461 aaagttctaa tcgaaagtga ttttgaccgt caacttatga attacaaaaa agatcaattc
     5521 gatttattac tcgaaaaatt tattgccatt caaacagaaa atacgaacat actgaatgac
     5581 tatgttcaca caaacaacca actcattgaa acacttatcg gaggaatata acatgagaaa
     5641 aacttctgtt caaaaacacg taacccttta tcccagtcac gttgacgttt tagattccat
     5701 catcgaaaaa aatccagacg tcaacaatta ttcagaagcc attcgttttt tatgtatgag
     5761 ttatgaacct gataccaata caaccacaac cagtaaccaa gcaaaactca atgccatggg
     5821 aaaagaaatt tctatcatga cagaaatcat ggcagaattt gctgatttat atctaaaaga
     5881 tactggtgtt cttatcggta caaattctaa agtgtatcaa gaagtgaaag aaaaaattga
     5941 aaatcaactc aatccttccc gacgaaatca acccaaaaat cgtcaaaaag aaaccaatca
     6001 aacctttact tctggaaatt ttgacccaac aaactttcta aaaagataaa aaaagaaacc
     6061 gatccctaaa aaaattcctc gatttctttt tagggatcag tcttccagca agatttgcaa
     6121 gttggtacca acttgcgaaa aaaaagaaaa atccgcttat gcaacttctt ctttttgtat
     6181 tttgaggcgc taccccaatc cccaagtatc ggttatttaa tagcgcggaa atccaaagaa
     6241 taggacgtac cctgaaacaa aaaaagactg ctctcacgag cagcccaaaa tgagattatc
     6301 ctattcatca acataaaaat attacaaagt gaagaactag agaaagacaa aaaaagcata
     6361 tcaaatcctc tcaaaaataa caagtcttta aaaaatgaca gataaaaatg cacaaagaac
     6421 taaaaaagag aaatcgcgta aaatcaagca ttagaagcta gtaactagct agaccaatta
     6481 aaaatcacaa agtgagcgtc tctatccatt ggtagagggg ctacttggca tagtgtacat
     6541 tatgttaact aacgctataa ataacgatta tgttcccaaa acaatatttt atgaactata
     6601 acaaaaattt gttatcgcat attgtatatt ttaattatag gtgtatacca cttaaattta
     6661 acacaaaggc gtgattattt tgaaacccaa aaagaaaatt tatctaggtc tcctcttttc
     6721 cttgatgatt ggaattttta gtataggaat tacaaatacc tatgcttcag aaaattctaa
     6781 tttcaatatc aactcccaac caattgaaga acaagcaatt aattttgcga aaactgattt
     6841 tgaacactcc ataacttcaa ttcttttaat ggaaaataac accgaaactg atattaacta
     6901 tactcttggt tcgccattta aaatgctcga tggagaaaat acggcaaata gttctatttt
     6961 ctttccagta ttttcagacg ctgataatac gattaaatat atttataccg tcaaaaaaaa
     7021 tacaaatgga gaatattcag gaagtatttc acaattttta gcaaaagaac ttcaaaatct
     7081 agtagatagt aatataacag atacaacctt attttttcaa gataatagca tttattattc
     7141 cgataatgga caaattaaac tactttggaa atcaccttta agtacagaaa aaaatggtgt
     7201 ttcttccttt acagaaaaaa atgatcttat atccgtaaat ataaaaaata aaattaaaaa
     7261 tttctctaaa aaacaaaatt ttcctgcggt taaacgtgta aaaagtgtct cctccggaga
     7321 taactttatt atgattaatt ggcgtatagc agaaacccaa acaagttttc cgtggtgtgc
     7381 tgcttacgta gaagctgcta tcttatgtaa caaaacagat gtacgtgcta cttcagctag
     7441 agccattatg cattatacct atcctaattt aaacaacagt caaattgaat caaaaagtct
     7501 taatcaagat caaataataa aatatgcaaa ttactgtggt gtttatccta aaaaagtagc
     7561 acgaatacta aacctttcag aggtccaaac acaactaaga aaaggaaatg ccatttatat
     7621 gggaactgct ggttatggaa attatagtaa cagccgtcat gcacttgcat tatttgggtg
     7681 ggtcaatcaa ggtggttatc aaacttacta tgtgtggaat ccttggtata actacccaac
     7741 tgtaataagt gcaaatgcag gaccagttac aattcctgta cctggaggtg gttttacttg
     7801 ggacaatacc attacaaatt ggtaaataaa cccattgc
//
