LOCUS       NZ_CP085904             2056 bp    DNA     circular CON 23-DEC-2023
DEFINITION  Enterococcus faecium strain SC1762 plasmid p10, complete sequence.
ACCESSION   NZ_CP085904
VERSION     NZ_CP085904.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22627481
            Assembly: GCF_020736585.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Zeng,W.
  TITLE     Acquisition of daptomycin resistance by Enterococcus faecium confers
            collateral sensitivity to glycopeptides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Zeng,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) Laboratory Medicine, College of Life
            Sciences, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 20:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,307
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 63 of 157
            Pseudo Genes (incomplete)         :: 95 of 157
            Pseudo Genes (internal stop)      :: 38 of 157
            Pseudo Genes (multiple problems)  :: 30 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085904.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="SC1762"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p10"
                     /geo_loc_name="China:wenzhou"
                     /collection_date="2018"
     gene            149..304
                     /locus_tag="LK894_RS16450"
                     /old_locus_tag="LK894_16465"
     CDS             149..304
                     /locus_tag="LK894_RS16450"
                     /old_locus_tag="LK894_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="WP_002299939.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANGK
                     ENEQKK"
                     /name="CopG family transcriptional regulator"
     gene            371..973
                     /locus_tag="LK894_RS16455"
                     /old_locus_tag="LK894_16470"
     CDS             371..973
                     /locus_tag="LK894_RS16455"
                     /old_locus_tag="LK894_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="WP_010729575.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="replication protein"
     gene            complement(1109..1366)
                     /locus_tag="LK894_RS16460"
                     /old_locus_tag="LK894_16475"
     CDS             complement(1109..1366)
                     /locus_tag="LK894_RS16460"
                     /old_locus_tag="LK894_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002377879.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(1367..1588)
                     /locus_tag="LK894_RS16465"
                     /old_locus_tag="LK894_16480"
     CDS             complement(1367..1588)
                     /locus_tag="LK894_RS16465"
                     /old_locus_tag="LK894_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002299940.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          complement(1032..1207)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.01e-87"
     Typing          372..901
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 ccaagggttt cagcgttttt ttttgacaaa atcagtaatg ccaagggttt aaggcctatt
       61 attcgctaat tagtatctaa actgttgcac cttagttact tttttgttac tatggttttt
      121 aagaaaaagt cgttggagga tacgaattat gatcagtaat gaaaaaaaga gagtcgttgt
      181 aacgcttact aaggaagtgg cagataatct tgataaaatt gcgaaacaaa tgggtcttag
      241 taagtctggt cttattacaa tttgggttaa tgctaacgga aaggaaaatg agcaaaaaaa
      301 atagcaaaag cattagtgtt ggcgcactgc ttttgctgtt agactgttgt tcaattaagg
      361 tgattatagc atgacgagta gaaaaactaa agatgaacgc acaaggaatt ggacgtttgt
      421 agtctatcct gaatcggctc ctgaaaattg gagggagttt ttagatgagt tacatgttcc
      481 ttgggttgag agtccacttc atgataaaga tgtgaatcct gacggtgaat tgaaaaagcc
      541 tcattggcat gttttgataa tgtttagcgg taaaaagacg tatgatcaaa tacgtgagat
      601 aactcaaaag attcggtctc caaacccgca aaaatgtgct aatgcaaaag gtatggtgcg
      661 atattttgct catatggata atccagaaaa atttcaatat gctaaaagtg atattatcgc
      721 tcatggtggt gcagaaattg ctagttattt gtcagtaacg agtgcagaac gatatgagtt
      781 gattagagag atgatgtcgt ttgttgattc taagaatatc actgaaatca aagatttaat
      841 tgactatgca atgtcagaaa gatttgatga ttggtttccg cttctttgtg ataattcagc
      901 ttatattatc gggcaatata taaaatcgaa tcgtcatggt ggaagtgtaa attcaaaaat
      961 aaataaagga taagagctgt taatttcagc tctttttttg tgcgtgaaag tcttagtcac
     1021 tatgaccaaa actacggacg aaactatgac caaaagtagg tactatatta tgaccgaaaa
     1081 taggtccaaa atggattttt ttgtgatatc aaaaaatata aggttgtttt gtaagaaaac
     1141 cagttggcac agccgactta gccgccttcc tgtaggaagg tgctatcagt tttcccttat
     1201 gctctttttg ttcttcagag tttaattcca gaatatttgt gagaagtagg tcaaagaggt
     1261 cggtggtttc ttcgactttt ccgccggtca attcgataaa atcattggaa tatgattcta
     1321 gcaatttttc tagttgttca acgttgatag ttgtttcttt aggcatttac tcaccttctt
     1381 tggtggttat gactttttct agttttttaa gggcttctgg gctgaaaaga tagtttcttc
     1441 taccggcaga gcgatattct tttccttcag tcagatgttc tttcgctaag cgcaaaacgt
     1501 aagcttggtt gtaatcaagt tcttgtgcaa gttcgctggt cgtcataatt tcggccaaag
     1561 tgaatttttc tgattttatc actctcattt atgctccttc catttaacgt ccgtctatat
     1621 tacgtgagta cgtcggactt acgacataag cttataatat aagttttctg tgttgtggtc
     1681 aattttatat tatcgaaatg actagtagaa aaaatcaatg ctctaaaaag ggtctatttt
     1741 gcgttttaag cgatttagcg tctatttcgt ttagttatcg gcgcaatcgt taaaacacgt
     1801 aagagaagcg agaatggagc ctgtaaggcg actggtagcg aaacggtcgt gttagattgc
     1861 aaaagccgat agctgaacga aatagtaagc gtggcgcctg catatatttc ggtagaagga
     1921 ggtcggggtt cggggcggag ccctgaggtt tgttttttgg ttttggtgtt tatgaattgg
     1981 gcggaggagg gggggactac gacacccccc ttattggtca ttggtcattg gtcgatagct
     2041 gtttagcctt actctc
//
