LOCUS       NZ_CP085899             7838 bp    DNA     circular CON 23-DEC-2023
DEFINITION  Enterococcus faecium strain SC1762 plasmid p5, complete sequence.
ACCESSION   NZ_CP085899
VERSION     NZ_CP085899.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22627481
            Assembly: GCF_020736585.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7838)
  AUTHORS   Zeng,W.
  TITLE     Acquisition of daptomycin resistance by Enterococcus faecium confers
            collateral sensitivity to glycopeptides
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7838)
  AUTHORS   Zeng,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2021) Laboratory Medicine, College of Life
            Sciences, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/23/2023 20:51:41
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,307
            CDSs (total)                      :: 3,216
            Genes (coding)                    :: 3,059
            CDSs (with protein)               :: 3,059
            Genes (RNA)                       :: 91
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 69
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 157
            CDSs (without protein)            :: 157
            Pseudo Genes (ambiguous residues) :: 0 of 157
            Pseudo Genes (frameshifted)       :: 63 of 157
            Pseudo Genes (incomplete)         :: 95 of 157
            Pseudo Genes (internal stop)      :: 38 of 157
            Pseudo Genes (multiple problems)  :: 30 of 157
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085899.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="SC1762"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p5"
                     /geo_loc_name="China:wenzhou"
                     /collection_date="2018"
     gene            complement(10..534)
                     /locus_tag="LK894_RS16250"
                     /old_locus_tag="LK894_16265"
     CDS             complement(10..534)
                     /locus_tag="LK894_RS16250"
                     /old_locus_tag="LK894_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_044383356.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINET
                     GQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQEK
                     DEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQS
                     KAEKKWWQFWQ"
                     /name="DUF536 domain-containing protein"
     gene            complement(551..1519)
                     /locus_tag="LK894_RS16255"
                     /old_locus_tag="LK894_16270"
     CDS             complement(551..1519)
                     /locus_tag="LK894_RS16255"
                     /old_locus_tag="LK894_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002347614.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="replication initiation protein"
     gene            complement(2690..3133)
                     /locus_tag="LK894_RS16260"
                     /old_locus_tag="LK894_16275"
     CDS             complement(2690..3133)
                     /locus_tag="LK894_RS16260"
                     /old_locus_tag="LK894_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(3106..4260)
                     /locus_tag="LK894_RS16265"
                     /old_locus_tag="LK894_16280"
     CDS             complement(3106..4260)
                     /locus_tag="LK894_RS16265"
                     /old_locus_tag="LK894_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_044383358.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPI
                     EEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDA
                     DNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIK
                     LLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDNFIMI
                     NWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKSLNQDQ
                     IIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQ
                     GGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            complement(4882..5298)
                     /locus_tag="LK894_RS16270"
                     /old_locus_tag="LK894_16285"
     CDS             complement(4882..5298)
                     /locus_tag="LK894_RS16270"
                     /old_locus_tag="LK894_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002305824.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKIEN
                     QLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
                     /name="hypothetical protein"
     gene            complement(5300..5581)
                     /locus_tag="LK894_RS16275"
                     /old_locus_tag="LK894_16290"
     CDS             complement(5300..5581)
                     /locus_tag="LK894_RS16275"
                     /old_locus_tag="LK894_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000353845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002347613.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(6103..7251)
                     /gene="tnpB"
                     /locus_tag="LK894_RS16280"
                     /old_locus_tag="LK894_16295"
     CDS             complement(6103..7251)
                     /gene="tnpB"
                     /locus_tag="LK894_RS16280"
                     /old_locus_tag="LK894_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(7268..7672)
                     /gene="tnpA"
                     /locus_tag="LK894_RS16285"
                     /old_locus_tag="LK894_16300"
     CDS             complement(7268..7672)
                     /gene="tnpA"
                     /locus_tag="LK894_RS16285"
                     /old_locus_tag="LK894_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     Typing          complement(556..1519)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.782"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 ttgctggttt tattgccaga attgccacca ttttttctcc gcctttgatt gtttttctat
       61 cgattctgtt tcttcagaaa gagtcgttga tttattttcc gaagattctt ccgtataagt
      121 caactgtagt tgttcctgca gtttctcgat ttgttggttg gcttgcaggg tgagttgttg
      181 ttggtggtcg agtaattttt gcatttgttt tatttgctcg tctttttctt gtagttgcat
      241 atctttttga tttaactgtt gttctaggag agaaaggact tctaattcgg gaacttggtt
      301 ggcgtttttg tggttttcgg ttttcgtttt ttcgccaact agttgttgat ttgagttttc
      361 gttttcgtta aaaaacattg attttatagc cttttgtccg gtttcattaa tcacaaactt
      421 gtttccgatt ttcgaaaacc aaagttttcg attttcgtca gaaacttttt tgttaattgc
      481 tgttttacta actcctaatt cttcagcaag ttctttgatt gttttttctg tcataaaatc
      541 taaaaacccc tcaatctttg aataagtttt gaagattgtc taataagtct ttttttagtt
      601 ctgaaggaat atcggaattt atctctgatt tttgtgcatt attttcttta ttttgggtat
      661 tttgttctct ttctaacttt tctaactgtt gtaaaagttc caattttcgc ttttgttctt
      721 tgattttttc ttgtaatgaa gtaggtgctt tttcagattg aacttgttgg ttagctagtt
      781 ctaatgcttt gttttgttct tcttttgttg gggcatgttt gttaaagtcg tctgattttt
      841 ttatctcagg tttaaatgta aattgatagc caatgacagg ctttccccgt ccttttccgt
      901 atttcttttt gattgttaaa cctttaaaga ttgcggttag ttcttgtcgt attggattga
      961 acacacgttg atcaatcgta ccttggttgt aacttttagg aatatcaagt aattcatgaa
     1021 agtcttcttt tgaaaaataa gcgaaacctt tggttctgta ttgttttagt aatcgaaaca
     1081 ttgtttttga ataactgctt tctaattcat tgaattgttg gagagaatag cgcacccatt
     1141 cttcaagatt attaagtaag ggtaaggctt tttcatgaac ttgtatttca gcaaaaggaa
     1201 tatcagattt tccgtcgatt ttaaattgat taaataaaac aaaacgttca cgttgcaaac
     1261 cgtcagcact tcttctacca aaacgtaaat ccattaattt gtcatacgtt gtttctaaat
     1321 catctatgaa tcgaacattt gcagttgctt tataattgct taaatctttt aattgctcga
     1381 atgtaaattg tactttttta tcacctacat cacgcattct tgatacgata gaaaagaaca
     1441 aattcatttc gactggtgta aattttcgca acggaattgt atttaattca tgatgatact
     1501 ttacgatttc attagacata tttaaaatag ctcctttctt actcgcacaa gagatacacg
     1561 ttatgaaata caaaatgcct gtttatctac atcttatatt aacatcgtcg atgtactaat
     1621 tcaatgtaca aacagacaaa acgatgtaga taaacagaca aaacgatgta gataaacaga
     1681 caaaacgatg tagataaaca gacaaaacga tgtagataaa agcgtcattc ccttgggaga
     1741 gtaaggatat agccgacggt aaagaggttt aaaaagaggt ttaaaaagag attataaaag
     1801 agggggacaa aatttcccgc ctaaaaaacc acttcttttt tctccaaaca gattcgcgca
     1861 tttttctcaa ctgggaatct tactccctac agtcgcttaa aaaggtgtga tcgctctgct
     1921 ctcacattat actgcgcagt cgcttccggc tcctttgcta aaaatgttca taaggaactc
     1981 tttgagaagc tatgagcctt cgtttgagac gttttaagac agtcttagta taaatccctt
     2041 atcttgttct taaagcgcct tacaggcggt cagggcaagc cctaacaacc ctaaagttgt
     2101 ctgaatgccc tctaaaattc gatttaagca ctcttaaacc tactcttata ttatttatat
     2161 acttagacgt ttcaaacgtc ttaaaacgca aattagagct tgataaagat atttgctcgt
     2221 gtgccgactt ttttaaaagt ctaggcacac gccatgacaa agctgaaata aactgcttca
     2281 gctttgtcat ggcggattcg ctcacttgaa aaagtgttca tttcgctcga ttattagcat
     2341 atatttttta actttttttg ctagccaaat tttgcattag ttcgctcata aatcccaccc
     2401 tttcagtacg tcctacggac gtatcgttcg taatgtgaaa gtacctttaa aggtactttc
     2461 tacaaaactc actaaagtgt ttggcaagct ctatatttgc tagccaaata cctacgtatt
     2521 tggtgcaaat aaccgctttt caaaggggcg caagctcccc cttgaatccc cctcaaaacc
     2581 cacgctccac aatacaaaaa aggctagcaa atgctagcca ctttttgtaa agtttcgctt
     2641 gtttcacctt cggtgaaatt attgttttgt aatatcaaac aactattcat caatcagctt
     2701 taaaaaattc aattggttta gctgtccctt ctgtcccatc attcgttgtc aaaataaata
     2761 cgagcttttt tggagaatcc aaactaaaag tgtaatctaa ttgtttttta ttagagcgca
     2821 gtgttagtaa attatttgtt gctttgtaaa tgaaaacttg ttcatttata tttaaatttt
     2881 tatcaatgat ttgaatttta tgattgttct cttttactaa gatggtatat tgttctatat
     2941 tttttgaagt atttaaatta gtgattacta gacgttctga atgatcttta gtttgccaag
     3001 ttcctagaaa aggaatcaga tcagtctttt gtatttcttg tttttttgaa caactagtta
     3061 agaaaagaac taacactaga cccataacta aagcaatggg tttatttacc aatttgtaat
     3121 ggtattgtcc caagtaaaac cacctccagg tacaggaatt gtaactggtc ctgcatttgc
     3181 acttattaca gttgggtagt tataccaagg attccacaca tagtaagttt gataaccacc
     3241 ttgattgacc cacccaaata atgcaagtgc atgacggctg ttactataat ttccataacc
     3301 agcagttccc atataaatgg catttccttt tcttagttgt gtttggacct ctgaaaggtt
     3361 tagtattcgt gctacttttt taggataaac accacagtaa tttgcatatt ttattatttg
     3421 atcttgatta agactttttg attcaatttg actgttgttt aaattaggat aggtataatg
     3481 cataatggct ctagctgaag tagcacgtac atctgttttg ttacataaga tagcagcttc
     3541 tacgtaagca gcacaccacg gaaaacttgt ttgggtttct gctatacgcc aattaatcat
     3601 aataaagtta tctccggagg agacactttt tacacgttta accgcaggaa aattttgttt
     3661 tttagagaaa tttttaattt tattttttat atttacggat ataagatcat ttttttctgt
     3721 aaaggaagaa acaccatttt tttctgtact taaaggtgat ttccaaagta gtttaatttg
     3781 tccattatcg gaataataaa tgctattatc ttgaaaaaat aaggttgtat ctgttatatt
     3841 actatctact agattttgaa gttcttttgc taaaaattgt gaaatacttc ctgaatattc
     3901 tccatttgta ttttttttga cggtataaat atatttaatc gtattatcag cgtctgaaaa
     3961 tactggaaag aaaatagaac tatttgccgt attttctcca tcgagcattt taaatggcga
     4021 accaagagta tagttaatat cagtttcggt gttattttcc attaaaagaa ttgaagttat
     4081 ggagtgttca aaatcagttt tcgcaaaatt aattgcttgt tcttcaattg gttgggagtt
     4141 gatattgaaa ttagaatttt ctgaagcata ggtatttgta attcctatac taaaaattcc
     4201 aatcatcaag gaaaagagga gacctagata aattttcttt ttgggtttca aaataatcac
     4261 gcctttgtgt taaatttaag tggtatacac ctataattaa aatatacaat atgcgataac
     4321 aaatttttgt tatagttcat aaaatattgt tttgggaaca taatcgttat ttatagcgtt
     4381 agttaacata atgtacacta tgccaagtag cccctctacc aatggataga gacgctcact
     4441 ttgtgatttt taattggtct agctagttac tagcttctaa tgcttgattt tacgcgattt
     4501 ctctttttta gttctttgtg catttttatc tgtcattttt taaagacttg ttatttttga
     4561 gaggatttga tatgcttttt ttgtctttct ctagttcttc actttgtaat atttttatgt
     4621 tgatgaatag gataatctca ttttgggctg ctcgtgagag cagtcttttt ttgtttcagg
     4681 gtacgtccta ttctttggat ttccgcgcta ttaaataacc gatacttggg gattggggta
     4741 gcgcctcaaa atacaaaaag aagaagttgc ataagcggat ttttcttttt tttcgcaagt
     4801 tggtaccaac ttgcaaatct tgctggaaga ctgatcccta aaaagaaatc gaggaatttt
     4861 tttagggatc ggtttctttt tttatctttt tagaaagttt gttgggtcaa aatttccaga
     4921 agtaaaggtt tgattggttt ctttttgacg atttttgggt tgatttcgtc gggaaggatt
     4981 gagttgattt tcaatttttt ctttcacttc ttgatacact ttagaatttg taccgataag
     5041 aacaccagta tcttttagat ataaatcagc aaattctgcc atgatttctg tcatgataga
     5101 aatttctttt cccatggcat tgagttttgc ttggttactg gttgtggttg tattggtatc
     5161 aggttcataa ctcatacata aaaaacgaat ggcttctgaa taattgttga cgtctggatt
     5221 tttttcgatg atggaatcta aaacgtcaac gtgactggga taaagggtta cgtgtttttg
     5281 aacagaagtt tttctcatgt tatattcctc cgataagtgt ttcaatgagt tggttgtttg
     5341 tgtgaacata gtcattcagt atgttcgtat tttctgtttg aatggcaata aatttttcga
     5401 gtaataaatc gaattgatct tttttgtaat tcataagttg acggtcaaaa tcactttcga
     5461 ttagaacttt tagatattta tttcgactcc attttttgcc cgttttggtt gtgagttcct
     5521 gacaacgttt atctattctt gcaataattt tgggattgat atttcgaact aaaatgtcca
     5581 tggaaatttt cctttcgtat cagcgtataa tttatgtata ccatacaaat atagtatttg
     5641 tatagctatt tttcgccaaa acactataca tagcctagcc acaatggcaa aaaagtgata
     5701 gcaaattttt ttctgagaaa cgtgataggc acagcctatc tagcaccttt cgtaagaaag
     5761 tgataccacg tttccttatg ctctttcaaa attcagaaaa atcagctgta aaacgagtgc
     5821 agtccgagta agattcaacg aaaaaacggg gattggggcg ccaccaaaat atcgcaacat
     5881 gggtccatgt tgctctgctc gcaaaagcaa aaagaagaat aaaaccagca atttttatta
     5941 atgtgaacta ctcaccactt agctaaacct gacggttctt aacgcttgaa gtgggagctt
     6001 cttgggaata gagcatactt gatagcgtat ttctttacta acagcggtgt ctcttattta
     6061 ccaagctatc cccgtagttc ctacggttct tgatgattgg atctaagcca atcccataga
     6121 atgtagtctt agaccttcgg tcagaatatt gatactagca ttgatatcac gatcatgatg
     6181 agtatgacaa acaggacaag tccattctcg aatttcaaga gatttcttgc catctttatg
     6241 tccgcactct gaacaaatct gactagacgg aaaccattta tctactttga tgatttttcg
     6301 tccataccag tcagccttgt attgtaattt agtcacaaac tttgaccaag aaacatcaga
     6361 aatactttta gctaatttat gatttcgcaa catacctttt gtgtttaagt cttcaataca
     6421 gataatatcg tgatttttga tcatttctgt actcaactta ttcagaaaat cagtacgttg
     6481 attcattact ttttcatgca atctagctac tttgcgtttt tgtttttgat aatttttagc
     6541 ttcaaataga ttggtacctt tatttttagc caataaagca cgcctagaca atttgcgttg
     6601 ttcacgcttt agtttctttt ccattttgga cgtgaattta ttattatcaa ttttttgtcc
     6661 atcagaaaga atcgcaaagt ccataatacc taagtcaata ccaattgtgg aattagtttt
     6721 aggtaattcg ataatttctt ctttacacaa caaagaaata tagtatttac cgctagaatg
     6781 acgtgatatt gtagcagatt tgataatccc ttttggttgt ctatgaagct taattcttac
     6841 taatgacttc aatttaggaa ctttgatgaa tttactatca atcaaagcaa ctgtaccatt
     6901 ttgattattc gttgtataac tctgaacagg atttttctta cttttgaaac gtggaaatcc
     6961 aacggattta tcccgaaaaa aattcttgta tgctttatct aaattaagtt gggcattggc
     7021 taaagcaagg ctatcaattt ctttcaaaaa cggaaattct ttcttgtatt tagctggtgt
     7081 cggaaaagtc atttttttag aagaatcatt tttaacttct tcatacgcct tttttcggtc
     7141 atcaagcatt agattgtaga ccttacggac acaaccaaaa gatttggcaa agaagatttc
     7201 ttgttcctct gttggataga ttctgaattt gtatgctttt agttgttcca taaagttcac
     7261 ctactttcta tttatgattg tttccttgat tttggatata cttcttaatc acatcaattg
     7321 gtgcgccacc agttgtcaga agacaaaaac ttttagacca gaacatttct ttccaaagaa
     7381 attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt ttataggcat
     7441 tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga acgtggtctt
     7501 tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt tcaaaagtag
     7561 ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc gttactaaaa
     7621 caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc atgttatatc
     7681 aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc aatacgcctt
     7741 agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt catcacccac
     7801 ctatagagga tgggcgactt ctgcctaatt ctgttaaa
//
