LOCUS       NZ_CP085870             4060 bp    DNA     circular CON 17-MAR-2024
DEFINITION  Klebsiella pneumoniae strain MV-u1-SK2-O plasmid pMV-u1-SK2-O-d,
            complete sequence.
ACCESSION   NZ_CP085870
VERSION     NZ_CP085870.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22551386
            Assembly: GCF_021952765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Schmitt,K.
  TITLE     Dissemination of OXA-48 Carbapenemase- and Extended-spectrum
            beta-lactamase-producing Enterobacteriaceae in a Swiss Companion
            Animal Clinic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x/40x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021952765.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 03:40:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,524
            CDSs (total)                      :: 5,401
            Genes (coding)                    :: 5,256
            CDSs (with protein)               :: 5,256
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 57 of 145
            Pseudo Genes (incomplete)         :: 88 of 145
            Pseudo Genes (internal stop)      :: 37 of 145
            Pseudo Genes (multiple problems)  :: 27 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085870.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="MV-u1-SK2-O"
                     /isolation_source="environmental swab veterinary clinic"
                     /db_xref="taxon:573"
                     /plasmid="pMV-u1-SK2-O-d"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020"
                     /collected_by="ILS, University of Zurich"
     gene            45..2192
                     /gene="mobQ"
                     /locus_tag="LKZ97_RS27545"
                     /old_locus_tag="LKZ97_27585"
     CDS             45..2192
                     /gene="mobQ"
                     /locus_tag="LKZ97_RS27545"
                     /old_locus_tag="LKZ97_27585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_015059934.1"
                     /translation="MAIYHLSMKIISRNSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKVAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQKWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRARIFERVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSLAARKVDFTQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNSQLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWDEW
                     RREEDARTHATVRRHDRGWSQ"
                     /name="mobQ"
     gene            complement(2438..3139)
                     /locus_tag="LKZ97_RS27550"
                     /old_locus_tag="LKZ97_27590"
     CDS             complement(2438..3139)
                     /locus_tag="LKZ97_RS27550"
                     /old_locus_tag="LKZ97_27590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_015059933.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCIGRLKSGADAESPKL
                     QKTMTITAAEYFDSFPDMGRKNAEVQLQEAIDRLWDRSIILKDDEKREEFRWIQYRAQY
                     AKGEARAQITFSDAVMPYLTQLQGQFTRVVIKNISRLSRSYSIRIYELLQQFRSTGERI
                     IALDDFRSMLDIEHKYQTYKSLNQQLLRPCIDELNKKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(3672..3926)
                     /gene="mobC"
                     /locus_tag="LKZ97_RS27555"
                     /old_locus_tag="LKZ97_27595"
     CDS             complement(3672..3926)
                     /gene="mobC"
                     /locus_tag="LKZ97_RS27555"
                     /old_locus_tag="LKZ97_27595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091744.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVIVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          45..403
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.444"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3928..4060)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.62e-63"
     Typing          complement(2440..3139)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4..2189
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.408"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 cctgcggggc tgaaagaaaa ccacaaaaag gcattacggc agaaatggcg atttatcatc
       61 tcagcatgaa aatcatctcc agaaacagcg gctacagcgc cgttgcttct gctgcctatc
      121 gttccggttc actgatgctt gatgagcgta ccggactgac tcacgactac acccgcaaaa
      181 gcggtgtggc tgaagctgtg attctgactc ccgcgacagc ccctgcctgg tgtacgaacc
      241 gtgccgaact ctggaatgcc gttgagaagg ccgaacgccg gaagaactca caacttgcgc
      301 gggaaattga actcgccatc ccccacgaac tgccgcagga tgccgcacgg gaaaccgttc
      361 tcgcctttgt cagagaaaac tttgtcagtc agggcatgat tgctgatgtg gcgttccatc
      421 acatggataa aacaaatccc catgcgcaca tcatgctgac cacgagagct gtcggacctg
      481 ctggattcgg tggaaaggtc agggactgga acgaccggac acatgccgag acgtggcggg
      541 catcatgggc tgaccatgca aacagggcac tggcgaacgc cggttatcag gaagagatag
      601 accatcgttc atacgagcga caggggcttg agaaaacgcc tggtattcac ctcggaaagt
      661 ctgcctgtgc gatggaaaca cgcggtatcg aaacagaacg cggcgaacag aatcgtctga
      721 ttaacaggct taacctggaa atacagattt cccgcaccga actcaggaac ggagggctga
      781 cagagcctgc ccgccgtgtg gctgaccttc tgaatatcgg gcttcctgac aacgcaactt
      841 cgtacacact acgtgacatc atcgaagcgt tgccgaagga cagtaactcg gcatggaaac
      901 tcaccagtaa tgccatgtcg atgatggcag atatgcaggc gacgcgcaga cgctgggaag
      961 agctgaacac cgaacgaaag gtagcgatga gccatgctgc cagcctgaaa aaatcatcgc
     1021 cgttttcgtc cggtttttcc cgtattcccc tgatgcaatg ggcggcaccg gaataccgta
     1081 gtgagcaggg aaaaatccag tctctgagcc agcaaatgga aaaattgcga cagcattacc
     1141 ggaaggtgga gaaacaggac attcccgcga gtcagatggc atttgaaaaa cagtggcaga
     1201 agtgggggga atccggcctt gcagctctca gagcccagtt gcttaaacgt gaagaggaat
     1261 tgcgccggaa ggaacaggaa gatactgaac gtagtcgcgc ggagcagctc aggaaaaatg
     1321 acaatgccgt tatggattca ggggtactga gcgcagtcgt tacggggtat ggcgaggccc
     1381 ccatgcctgg taacggggaa atgacctgtt accttctgct tcacaaccgg aacggagaat
     1441 atacgctctg ggggaacgaa ctggagaaat accgcgccag aatctttgag cgtgttgatt
     1501 taatgcgtga caggagtggt tatatctgtg accgaagcga aatcggccaa cgtccgccac
     1561 tgcaacgtgt ttattcctct gcgacctttg agcaactgct gacgcaggtt tgccagacat
     1621 ggccgcaaca tacccgcaat ctccgccagc cgaagacatg gccggagtct ttctgtcttg
     1681 gagaagaccg ccagccagcc atgccatcac tggcagcaag gaaggtggat tttacgcagg
     1741 gacgtctgcc accgacgctg atgccggtaa tgagttctgt agaccgggaa acccgacaac
     1801 ttcagctttt gctggtcatg ggcgtggatg actctctggg cggtgttgtc cggcttaatg
     1861 gtacgctgta tcccgcgttt gccgttccct cagccgataa cagccagctg gtcatcagtg
     1921 cactgaccga taaggggtta cggtatgcag ggtatggtgt tgccgtaaat cacgatgcag
     1981 acagccacat atcacctgca ccagaactga tgcagtttca tctgaaaacc cgtgaggcac
     2041 cgctttttgc tgccgtcaac acaccagaaa aacagccgga tcatctgttc cgctcgctgg
     2101 gttttaatcg tacctgggac gaatggcggc gcgaggaaga tgcacgaaca cacgccaccg
     2161 tgaggagaca tgatcgcggt tggtcacagt gaatgtgaac aatattttgt tttgtgtaac
     2221 atcgtgttat gttcgccgat gttcatcgac agggatgaat gcaagaagca cactcacata
     2281 acgcaaacat agttttgttt ttccgccccc aaaattgggg gttctttttt ggcctctcaa
     2341 atcaattctg acgcaattta ttgccaaagc agtaccatcg catacctgac accaaaaaaa
     2401 cgcaccagag aacgttttag aggccttaaa atcgattcta gatcgtcatc ttgatctgtt
     2461 tgtcttcctt gaagcgaaaa tgcagggcta caaccgtgcg cccctttttg atcgtctcaa
     2521 ccgttacaac cagatcgctt tttttgttca gttcgtcaat gcagggtctt aataattgct
     2581 ggttaagtga tttatatgtt tgatatttat gctctatatc aagcattgag cgaaaatcat
     2641 cgagagcgat aatacgttcg ccagtactgc ggaactgctg gaggagttcg tatatcctta
     2701 tactgtaact tcttgataat ctagatatat tcttgattac aactctagta aattgccctt
     2761 gtagttgtgt caaataaggc attactgcgt cagaaaatgt tatttgtgct ctggcctcgc
     2821 ctttagcgta ctgtgcccga tactggatcc agcggaactc ctcacgtttc tcgtcatcct
     2881 tgagaatgat tgacctgtcc cacaaacgat caattgcttc ctgtaactga acctcggcat
     2941 ttttccgccc catgtcaggg aatgaatcga aatattccgc agctgtaatg gtcattgtct
     3001 tttgcaattt tggagattct gcatcagccc cagacttcaa tcgaccgatg caaagtaaca
     3061 aaaatcgctg ttcctgtagg gtcaatttat aactggcttc aaccagcgca ttagatttat
     3121 aggcaattag atccgacata gagcatccct ccccaaaagg taatgaataa aagataaaca
     3181 tacccccagc gaaaggcaat gcctttttat tttaatatcc ctttttctgt gaatattacc
     3241 ttaaatcata ttacctttac cttaaatcat attacctaaa caccttaaat catattacct
     3301 ttaccttaaa tcatattacc tatagcgttg taacattttg ttttctataa ggaattttgg
     3361 cactaaaaca agtattaaaa acaagatctt aaaaacaaga tctaaaaact taacgacgag
     3421 cctgtggata acttggagtg ctgaaaacaa aaaaccaata aaaacaatgc gttgcaaaaa
     3481 agcagaattg caagttccga atacaccccc tccaaccctg tggaaatcca gtattcatgc
     3541 gggttcagcg catatcacct cccgaaaaac cgtgcgtatg tgctcaatca ggcgacggat
     3601 tcacttccgc ttcctcgccc actcgctcgc taacgctcgg tcgctgggac tgcggcgagc
     3661 ggacgataca atcactgttc tggcagcgct tccagctcgt taatcagcgg cagtatgcgc
     3721 gtcacatcag cctgtcgtgt cacctctgcc ttcaggaact ggagtaacca ggcacgcaac
     3781 tgcacctcct ccggctttct gacctttgcc agcaatgctg cacccacaat gaccttctgc
     3841 gctgtgtcca gactcctgct cttcgccttc aggcgctgta atctggcctc agcatcagca
     3901 atcttctgtt cgagtgttct gctcatgatg taactccgtg cgcgatgaaa aatcgcattt
     3961 tagcgcgtca ctggtagttt aaaaactgag ctggcataat gcacggcaaa tcacgaagtg
     4021 cgcacttata caatctccac tccgtttcga ttgtgtgcgc
//
