LOCUS       NZ_CP085869             4667 bp    DNA     circular CON 17-MAR-2024
DEFINITION  Klebsiella pneumoniae strain MV-u1-SK2-O plasmid pMV-u1-SK2-O-c,
            complete sequence.
ACCESSION   NZ_CP085869
VERSION     NZ_CP085869.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22551386
            Assembly: GCF_021952765.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Schmitt,K.
  TITLE     Dissemination of OXA-48 Carbapenemase- and Extended-spectrum
            beta-lactamase-producing Enterobacteriaceae in a Swiss Companion
            Animal Clinic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 40x/40x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021952765.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 03:40:16
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,524
            CDSs (total)                      :: 5,401
            Genes (coding)                    :: 5,256
            CDSs (with protein)               :: 5,256
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 14
            Pseudo Genes (total)              :: 145
            CDSs (without protein)            :: 145
            Pseudo Genes (ambiguous residues) :: 0 of 145
            Pseudo Genes (frameshifted)       :: 57 of 145
            Pseudo Genes (incomplete)         :: 88 of 145
            Pseudo Genes (internal stop)      :: 37 of 145
            Pseudo Genes (multiple problems)  :: 27 of 145
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085869.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="MV-u1-SK2-O"
                     /isolation_source="environmental swab veterinary clinic"
                     /db_xref="taxon:573"
                     /plasmid="pMV-u1-SK2-O-c"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020"
                     /collected_by="ILS, University of Zurich"
     gene            complement(join(4565..4667,1..92))
                     /locus_tag="LKZ97_RS27510"
                     /old_locus_tag="LKZ97_27560"
     CDS             complement(join(4565..4667,1..92))
                     /locus_tag="LKZ97_RS27510"
                     /old_locus_tag="LKZ97_27560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072021023.1"
                     /translation="MLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMQRENAALSERVT
                     GLSRQVQSLSEQLRRLSSD"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(<55..141)
                     /locus_tag="LKZ97_RS27515"
                     /pseudo=""
     CDS             complement(<55..141)
                     /locus_tag="LKZ97_RS27515"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(117..602)
                     /locus_tag="LKZ97_RS27520"
                     /old_locus_tag="LKZ97_27565"
     CDS             complement(117..602)
                     /locus_tag="LKZ97_RS27520"
                     /old_locus_tag="LKZ97_27565"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_045326458.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQSTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            complement(<637..1341)
                     /locus_tag="LKZ97_RS27660"
                     /old_locus_tag="LKZ97_27570"
                     /pseudo=""
     CDS             complement(<637..1341)
                     /locus_tag="LKZ97_RS27660"
                     /old_locus_tag="LKZ97_27570"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(1280..1603)
                     /locus_tag="LKZ97_RS27530"
                     /old_locus_tag="LKZ97_27575"
     CDS             complement(1280..1603)
                     /locus_tag="LKZ97_RS27530"
                     /old_locus_tag="LKZ97_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_007772081.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(2663..4219)
                     /locus_tag="LKZ97_RS27535"
                     /old_locus_tag="LKZ97_27580"
     CDS             complement(2663..4219)
                     /locus_tag="LKZ97_RS27535"
                     /old_locus_tag="LKZ97_27580"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013199864.1"
                     /GO_function="GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]; GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="WP_045326453.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLD
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVFV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPFKSVTGNLIILLSHPIYD
                     KNGNYIGHVGGSIYLKKHSLFSDVLSRHFFKSDTEISIVSDDGTVIFNNDNSAVGRPMV
                     MSDDLKSKLTESDRGEGEFFFSGNRYLLGYAHMKNTAWNVLVYSHADSATTILITYAKS
                     VLVLLIVIIITFSLLSYFIASRISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTHVKVMMERVDSLNEVALKDPLTGVNNRLGFSTKIQNNKYGAGASVIAIDVDFF
                     KKINDSFGHGVGDEVLVSLAQIIKSCCRSEDIICRFGGEEFVVFLPNTSVVTAECVAER
                     IRSVIERTVFPNNLRVTVSAGVATQDDPLGGIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="sensor domain-containing diguanylate cyclase"
     gene            complement(4458..>4622)
                     /locus_tag="LKZ97_RS27540"
                     /pseudo=""
     CDS             complement(4458..>4622)
                     /locus_tag="LKZ97_RS27540"
                     /inference="COORDINATES: protein motif:HMM:NF016769.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /name="nuclease"
     Typing          complement(1717..1889)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.126"
                     /coverage="100.0"
                     /evalue="1.99e-77"
     Typing          1814..2366
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.639"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          complement(2..1290)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.558"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1287..1603)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.396"
                     /coverage="99.0"
                     /evalue="2.47e-61"
ORIGIN
        1 ccagacatcg tccgccattc cgcgaaggcg ctctcccact cgtccagcct tttcgagtag
       61 tcctgctgta gctgctctaa tgcgctcagc aactgttttt ccagctccgt catgtgctat
      121 ttccccgcca gtatcatcca gctcgaatcc tctccgaacc gtcccgcttc cgggttcacc
      181 ttcacgcagc ggcgtctctg ctcgccgcaa gtcgagagct gcacgccgct gttcctctct
      241 gacagcatgg cctgcgtgct cttctgctcc cggatggtcg tgtagttgtc gagtatctgc
      301 tgcccctgcc accacagaat gcccgcgctc gaggcgatca gcagcgcgga gaccaggacg
      361 atggtcagcc acgtctggct gaccatgcgc accatgcctt tcgtgcgctg gctcatggct
      421 gaggacagtt tccggtcgtg gtcgaggatg gcggcgctga ttctcttcgc gctttcgttc
      481 agttccgctt ttacagattt ctcgtgctcg ctgaatgcgg ctttcagcat ctcgccggta
      541 ctctgctgct gcgctttcga tttctgctct aagtccttcg ccagcgttaa aagactgttc
      601 ataaatggct cccttcagtc ggatgtttcg ccccccgtcc gggtcggcaa tgctgatact
      661 gctttttgtg gtgcggacca cctcaaaacc tgcgctttcc agcgcctcag tgacatcctg
      721 acgggttttc agctccccgg atgaggcaag agcaagcaga ccgcgtgtaa tcgcttctgc
      781 agcctcctgc ttcgctttag gcagtgcaga cggtgtgacc agtgcccgcc ggttctccgg
      841 tgcgttcggg tcgtgcagcc ccagtctgcc gttcacgatg gtctgccagg catcgatgcg
      901 tggacggtcg gcgcgatcgt aatacggctg gagccgtctg ccggtcagca gctccgtgtt
      961 cgggatcaga aaattcagct caagccgccc cttgtcacga tgctccaccc acaggatgct
     1021 gtactggtct ttatcaagtc cgggcatcag aacccgctca aagcttgcca tcagcttttc
     1081 ccgctggccg gagggtaaat ccgcttccgc aaacgacagt accccggagg tgtatttctt
     1141 aacgtaaggc gacgcgtcaa tcagctcccg gacttcttcc ggcttcccct gcaggacggt
     1201 ggcgccttca cgctgccggt ctttacccag cagatagtcc accggcccgg cgccaccgcc
     1261 gcgaccgcgt ggatgaaatt taacgatcat cgtccgttcc tttctcaaga acggcgtgcc
     1321 gcagccgctc aagtccggca tcgatggcca tcagcgccgc aacaatctgc accctgtcat
     1381 ggccggtacc gccgccggcg ttaacacggc gggctatctg gttcaggtta ttgcccatgc
     1441 cggcaagctg gcgcagcagc gccggcgaga ttgagggaag ctttccggaa cgtgccggct
     1501 tttcgtccag gcaggtctgc cgcatccacg ctgccagctg cctgccgtcg cagcgctcaa
     1561 gcagccgccg gtgctcgtcc tccgtcaccc acatggtgag catcttgttc cgtttctctg
     1621 acaaaccggc ctcctgataa cgccgggacg ggcgggaaag cctttcccgg tcggcgcggg
     1681 gttggacaag ccgcaggcgc gtcaggcttt tggcgggttt cggggcgcag ccctgaacca
     1741 gtcacgtagc gctagcggag tgtatactgg cttactatgt tggcacggat gcgagtgtca
     1801 gtgaaatgct tcatgtggca ggagaaaaat gcagcagcgg agcgtcagca gaatatgtga
     1861 tacaagatat attccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     1921 cgagcggtac cggcttacag gcggggcgga aatttcctgg gagatgccag gaagaagctt
     1981 aacagggaag cgataaggcc acggcgaagc cgtttttcca taggctccgc ccccctgaca
     2041 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     2101 accaggcgtt tccccctggt ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     2161 ccggtgtcaa tccgctgtta tggccgcgtt tatctcattc cacgcctgac actcggttcc
     2221 gggaaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gcccgactac
     2281 cacgttgtcc cggtaactat catcttgagt ccaacccgga acagacacga aaaatcgcca
     2341 gtggcggtag ccattagtaa gaggattcac ggagcggagt agtagagtgt tctacagagt
     2401 tcttgaagtg gtggcctgac tgcggctaca ctggaaggac agttttggta cctgcgctcc
     2461 gtgaaagcca gttaccttcg gaagaaagag ttggtagctc ttgattccgg caaacgaacc
     2521 accgttggta gcggtggttt ttttgtttgc agggcatgag attacgacga catcgaaggg
     2581 ataacaagag gatccgcttc ttgaaccccc ctccccacaa aaaaaaacgg cacactagcg
     2641 cgtaaagcgt catcaaaaaa actcaaatag cgctatatac aatcacttta ttacgcccct
     2701 ctctcttagc ttgataaaga gcatcgtcag catttttcag caaaaaatcg atcccaccaa
     2761 gaggatcatc ttgtgttgcc acaccagcag atacggtgac acgcaagtta ttagggaaaa
     2821 cagtacgttc aataacactc ctaatacgct ccgcaacaca ctccgctgtt accacagatg
     2881 tattcggcag aaaaacaaca aactcttctc caccaaaccg acaaataata tcttcactac
     2941 ggcaacaaga cttaataatc tgagccaaag aaacaagcac ctcatctccg accccatgcc
     3001 cgaaactatc atttattttt ttaaaaaaat caacatcaat agcaataact gaagctcctg
     3061 ccccatactt gttattctgt attttagttg aaaaccccaa tctattgttc acgccggtaa
     3121 ggggatcttt aagcgccacc tcatttaaag aatcaaccct ttccatcatt accttaacat
     3181 gcgtaaaaag tgccttttta agtcgatcag cttcggcata ccaagcattg atagctttaa
     3241 tataaactaa cgaagcccct atatctttag cattggtaga tatagcaagc ttttctaaag
     3301 gaagtgaaat acgggaggca atgaaatatg aaagaagtga aaatgtaata attattacaa
     3361 ttagcagaac aagtacactt ttagcataag taataagaat agtagtcgcg ctgtcagcat
     3421 ggctataaac caacacattc caagcagtat ttttcatatg agcataaccc agaagatatc
     3481 tattccccga aaaaaaaaat tccccctcac ctctatcaga ctccgttaac tttgatttca
     3541 aatcatcaga cataaccatg ggccgaccaa cagcactgtt atcgttatta aagatcacag
     3601 ttccatcatc actaacaatg ctaatttcag tgtcgctctt gaaaaaatgc ctgctcaaaa
     3661 catcgctaaa taaactatgt ttttttagat atatagaccc accgacatgc cctatgtaat
     3721 tgccattctt atcataaata ggatgtgaca acaaaataat caaattccca gtaactgatt
     3781 tgaacggtga agatatgaat ggtttctttt cctcaatcgc caatttgctg gcacttgaat
     3841 tcaagcgggt accaactaaa tcaagagatt ctggcgaagt cgccagaaca acagcatcat
     3901 tactaacaac aaaaactgaa ttaaacatac cagactgcaa tcttaaccta tccgcctcat
     3961 ctttaagtga gtcaatatca ttagttgatt ttattttttt tgctccatat gctaattcac
     4021 cttgagccat ttcaatataa ccactcatta catctgacat tttcatagcg tacgatgaat
     4081 tagcatcaag caaatctttt tctatatttt cgcgctgaaa aaccataata acggcaatca
     4141 gaaaaataga agttaacaaa atcccaccaa cggacaaggt tatgactatc ggacgaaggt
     4201 gaggcttttt aattctcata aaaatacact ccatataata aaaaatgccg catttaccat
     4261 aacatccctt atcagcaccg ccccggacgg gttcggaaca aatgcggcgt cagcccggta
     4321 aaaccggcag caaactctcc gctaaaccgc gcattctgcg ccagataaat ttcacccggc
     4381 tgcatggcta tgcagtcggg tgaatggctc cggctttcag cgcagatgtc caccgaaagc
     4441 cccgttttat caggttatca cagtgaaggg ctgttatacg tccgctgtcg ctgagatcgc
     4501 tccagctcca gcgctttctg atgctgcacc acgcgctcct gctcccggtg cagctcgacg
     4561 gcattcagtc gctcgacaac cggcgcagct gctcgctcaa actctgcacc tgcctgctca
     4621 agcccgtgac gcgctcgctc agcgctgcgt tctcccgttg cataaga
//
