LOCUS       NZ_CP085865             5891 bp    DNA     circular CON 17-MAR-2024
DEFINITION  Klebsiella pneumoniae strain MV-v4-SK2-O plasmid pMV-v4-SK2-O-b,
            complete sequence.
ACCESSION   NZ_CP085865
VERSION     NZ_CP085865.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22551388
            Assembly: GCF_021952745.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Schmitt,K.
  TITLE     Dissemination of OXA-48 Carbapenemase- and Extended-spectrum
            beta-lactamase-producing Enterobacteriaceae in a Swiss Companion
            Animal Clinic
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Biggel,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2021) Institute for Food Safety and Hygiene,
            University of Zurich, Winterthurerstrasse 272, Zurich 8057,
            Switzerland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x/30x
            Sequencing Technology :: Illumina MiniSeq, Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_021952745.1-RS_2024_03_17
            Annotation Date                   :: 03/17/2024 03:27:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,243
            CDSs (total)                      :: 5,120
            Genes (coding)                    :: 5,034
            CDSs (with protein)               :: 5,034
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 45 of 86
            Pseudo Genes (incomplete)         :: 41 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 13 of 86
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="MV-v4-SK2-O"
                     /isolation_source="environmental swab veterinary clinic"
                     /db_xref="taxon:573"
                     /plasmid="pMV-v4-SK2-O-b"
                     /geo_loc_name="Switzerland"
                     /collection_date="2020"
                     /collected_by="ILS, University of Zurich"
     gene            332..490
                     /locus_tag="LKZ99_RS26240"
     CDS             332..490
                     /locus_tag="LKZ99_RS26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071529495.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDKAPLAAATGNWTRWIEIL
                     RVLEVVA"
                     /name="replication initiation protein"
     gene            809..1621
                     /locus_tag="LKZ99_RS26125"
                     /old_locus_tag="LKZ99_26195"
     CDS             809..1621
                     /locus_tag="LKZ99_RS26125"
                     /old_locus_tag="LKZ99_26195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="WP_000549944.1"
                     /translation="MHLEEGDLRALERAVELLEQDTITETLTQLVGTPIDYLMSKLPKG
                     AEEQIYSLVEKSLHKAADAALWSLDNEPNRIASTKTNKFFAAVSGAIGGAFGFSALAIE
                     LPVSTTIMLRSIADIARSEGFELDNIDTKLACLEVFSLGGPSDDDDAANTAYYATRSLT
                     AEIVQDLSKEISNIAVKNASSVQTGKWLASLIEKVATRFGIVITEKMAAQVVPVIGALT
                     GATLNTMFTDYYQDMARGHFIVKRLEKKYGNELIKAEYIKISSKLTNS"
                     /name="EcsC family protein"
     gene            1849..2283
                     /locus_tag="LKZ99_RS26130"
                     /old_locus_tag="LKZ99_26200"
     CDS             1849..2283
                     /locus_tag="LKZ99_RS26130"
                     /old_locus_tag="LKZ99_26200"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_021551691.1"
                     /translation="MGFWDSIKNAAIKAKCGVGIHGGNYKLIDGETCKYSKLCPDCNRT
                     IQKEQHKYGEESYKYDFKCTTVKKCIDCGAEQEGERHERFVEIAVDDYCNVKERCVRCF
                     TERVHGKRHNWYLSGSSDTYRHYKCSVCGEEKEERKTSFR"
                     /name="hypothetical protein"
     gene            2319..3203
                     /locus_tag="LKZ99_RS26135"
                     /old_locus_tag="LKZ99_26205"
     CDS             2319..3203
                     /locus_tag="LKZ99_RS26135"
                     /old_locus_tag="LKZ99_26205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439342.1"
                     /GO_function="GO:0005525 - GTP binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="WP_021551692.1"
                     /translation="MENNTQGIKLIKEAVDVLPKEIRKSVYNKIRNVINYEPVIGIMGK
                     TGAGKSSLCNSIFKGNVCAVSDVEACTRKVQELIISFGDRTIKIIDIPGVGENEAKDIE
                     YEELYKNLLPELDLVLWVVKGDDRAFSADEHFYKNVLKPVGGDEKVLFVLNQVDKIEPF
                     REWDEEKHQPSPSQQINIRKKEEYLIERFGFTKHPVISVSANEGYNISRLVEAIVRSLP
                     KEAQSGVVHQVKDEIKTKEIVETAKDGFADTVDRAIDDIIDTVLPKPIASVAKVAKKAI
                     VSVAKSVWGLLFG"
                     /name="GTPase"
     gene            complement(3346..3507)
                     /locus_tag="LKZ99_RS26245"
     CDS             complement(3346..3507)
                     /locus_tag="LKZ99_RS26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="WP_071779130.1"
                     /translation="MRYPWNGSVSWKYRNAHWMALRWDGETMKNGRFIGLRRTNVLCAI
                     WLHRYSYG"
                     /name="mobilization protein"
     gene            complement(3538..3771)
                     /locus_tag="LKZ99_RS26140"
                     /old_locus_tag="LKZ99_26210"
     CDS             complement(3538..3771)
                     /locus_tag="LKZ99_RS26140"
                     /old_locus_tag="LKZ99_26210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137445.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3778..4296)
                     /locus_tag="LKZ99_RS26145"
                     /old_locus_tag="LKZ99_26215"
     CDS             complement(3778..4296)
                     /locus_tag="LKZ99_RS26145"
                     /old_locus_tag="LKZ99_26215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_000067499.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTVSTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEMIPFQ
                     GKEWVHLKE"
                     /name="MbeB family mobilization protein"
     gene            complement(<4334..4984)
                     /locus_tag="LKZ99_RS26150"
                     /old_locus_tag="LKZ99_26220"
                     /pseudo=""
     CDS             complement(<4334..4984)
                     /locus_tag="LKZ99_RS26150"
                     /old_locus_tag="LKZ99_26220"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(4974..5297)
                     /locus_tag="LKZ99_RS26155"
                     /old_locus_tag="LKZ99_26225"
     CDS             complement(4974..5297)
                     /locus_tag="LKZ99_RS26155"
                     /old_locus_tag="LKZ99_26225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_000955996.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPTL
                     APPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQGAR
                     DDS"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            5362..5553
                     /locus_tag="LKZ99_RS26160"
                     /old_locus_tag="LKZ99_26230"
     CDS             5362..5553
                     /locus_tag="LKZ99_RS26160"
                     /old_locus_tag="LKZ99_26230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165993.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHEHA
                     DELYRSCLSRFGDDGENL"
                     /name="Rop family plasmid primer RNA-binding protein"
     Typing          complement(5639..5724)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="8.58e-38"
     Typing          2..404
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.062"
                     /coverage="100.0"
                     /evalue="1.03e-179"
     Typing          complement(3435..4984)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.702"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 acagaatcag gggataaggc cggaaagaac atgtgagcaa aaaggcaaag ggcaacgaaa
       61 ggccgcgccg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
      121 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
      181 tggtggctcc ctcgtgcgct ctcctgttcc gaccctgccg tttaccggat acctgtccgc
      241 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcggccc
      301 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
      361 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acaaagcgcc
      421 actggcagca gccactggta actggacaag gtggattgag atattaagag ttcttgaagt
      481 ggtggcctaa ctgcggctac actagaagga cagtatttgg tatctgtgct ccaccaagcc
      541 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgttggtag
      601 cggtggtttt tttgtttaca ggcagcagat tacgcgcaga aaaaaaggat cacaagagga
      661 tcctttgatc ttttcatcac tcctgcttca cagacaacaa tgatgtcggc atctcgccta
      721 ttgaatcact gaactcgctc aaggtaagct cccaagacga atcacttaca aaagtagact
      781 atcgattgat tcataaagga ttcattatat gcatcttgaa gaaggagatc tgcgggcttt
      841 agaaagggcc gtagaattat tagaacaaga tacaattact gaaacattaa cccaattagt
      901 tggaacccca atagattact taatgagtaa acttcccaaa ggggcagaag agcaaattta
      961 tagtctagta gaaaaatcgc tccacaaagc tgctgatgcg gccttatgga gtctagacaa
     1021 tgaacctaac cgaatagcat caactaagac aaataaattc tttgcggccg tatctggagc
     1081 cattggtggc gcttttggct tttctgcatt agcaattgag ttaccggttt caacgacaat
     1141 tatgcttcgt tcaattgctg acatagcaag aagtgaaggt tttgagctag ataatattga
     1201 taccaaactg gcctgcttag aggtgttttc cttgggagga ccatctgatg acgatgatgc
     1261 agctaatact gcatactatg caacaagaag tttgactgcc gagatcgtac aagatttatc
     1321 caaagaaatt tctaacattg ctgtcaaaaa cgcatcttca gtacaaacag gaaaatggtt
     1381 agcttcgtta atagaaaaag ttgcaacaag atttggtata gttattacgg aaaaaatggc
     1441 agctcaagtc gttcccgtta ttggagcact aactggagcc acattaaaca caatgtttac
     1501 cgactattat caagatatgg ctcgaggtca tttcattgtt aaaagactcg agaaaaaata
     1561 tggcaatgag ttaataaagg ctgaatatat aaaaatctca agcaaactca caaattcgta
     1621 aaatatttat cttggatgaa ttagacaatg actttccaat actatctttg cgggaaaaac
     1681 acgaagttta tccagtgccc ccactttttc agcgctatat ttaacataac aaaaacatta
     1741 tacagagcat aaatccatac tcatttttat atatccacac actatacgtg tataatcatg
     1801 tcgatgaatt gttcaactta tatcaactaa tcgtagggag atttttccat gggcttttgg
     1861 gatagcataa aaaatgcagc aatcaaggct aagtgtgggg taggtataca cggaggaaac
     1921 tataagctta ttgatggtga gacttgtaaa tattctaaac tatgccctga ctgcaatcgc
     1981 actatacaaa aagaacagca taaatacgga gaggaaagtt ataaatacga tttcaagtgc
     2041 acaactgtaa aaaagtgtat cgactgtggc gctgaacaag aaggcgaaag gcatgaaaga
     2101 tttgttgaaa ttgcagtcga tgattattgc aatgtgaaag aaagatgcgt gagatgtttt
     2161 acagagcgag ttcatggtaa aagacacaat tggtatctta gtgggagttc tgatacctat
     2221 cgccattaca aatgttctgt atgcggggaa gaaaaagaag aaagaaaaac aagcttccgt
     2281 taattcatat gaacaacaaa atgaggaaaa tagtaactat ggaaaacaat acgcaaggta
     2341 taaaattaat caaagaagct gtggatgttc tacctaaaga gattcgcaag tctgtctata
     2401 acaaaattag aaatgttatt aattacgaac cagtaatagg gattatgggt aaaaccggcg
     2461 ctggtaaatc cagcctatgt aattctatct ttaaaggtaa tgtatgtgca gtgagtgatg
     2521 tagaagcatg cacgagaaag gttcaggaat taataatttc atttggtgat agaacaatta
     2581 aaataataga tataccaggt gtaggtgaaa atgaagctaa agatatagaa tatgaagagt
     2641 tgtataaaaa tcttctccca gaattagatc tggtgctttg ggttgttaaa ggtgatgatc
     2701 gagcattttc agctgatgag cacttttata aaaatgtttt aaaaccagtt ggtggtgatg
     2761 aaaaagtgtt gtttgtttta aaccaagtcg acaagattga gccattccgc gaatgggatg
     2821 aagaaaaaca ccaaccatca ccctcacaac aaataaatat tagaaaaaag gaagaatact
     2881 taatagaacg attcggtttt accaaacatc ccgttatatc tgtatctgcg aatgaaggtt
     2941 ataacatttc aaggttagtg gaggcaatag taagatcgct cccaaaggaa gcacagagcg
     3001 gagtggtgca tcaagtaaaa gacgaaataa aaacaaaaga aattgtagaa acggcaaagg
     3061 acgggttcgc agacaccgta gatagagcta ttgatgatat aatagacacc gtactaccta
     3121 aaccaatagc gtcagtggct aaagtcgcca agaaagccat tgtttctgta gctaaaagtg
     3181 tttggggatt gctcttcgga taatcaagca ggaaagccca tcagtattta acataacaac
     3241 agtttgtacg taccgctaaa acgcgttcag cgcgatcacg gcagcagaca ggtaaaaatg
     3301 gcaacaaacc acccgaaaaa ctgccacgat cgcgcctgat aaattttaac cgtatgaata
     3361 cctatgcaac cagatggcac agagcacatt agtccgcctt aatccaatga acctgccatt
     3421 tttcatggtt tcaccatccc agcgaagggc catccagtgt gcgttcctgt atttccagct
     3481 gacgctcccg ttccagggat aacgcatgtt cgcgctcctg catcaaccgt tcctctctta
     3541 tctccagttc tcgctgtata actggctcaa gcgttctgtc tgctcgctca agtgctgcac
     3601 ctgctgactc aactgcatga cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga
     3661 aaacatcttc tgcaattcca cgaaggcgct ctcccattcg ctcagccgct gcatatagtc
     3721 ctgttgcagc tgctctaagg cgttcagcaa atgtgtttcc agctctgtca ctctgtgtca
     3781 ctccttcaga tgtacccact ctttcccctg aaagggaatc atctctgctg atttcccgta
     3841 cggaaggacc agaaatttcc tgttcccgtc ctgcacaaac tccacgcccc atgtcttcgc
     3901 gttcagtttc tgcaatgtct cttcctgctt cctgatttct tccaggttcg tctgtatccg
     3961 tccgccaaga taccagagcg tcccgccact cgcggtaaac agaagaaaga ctatccccag
     4021 taacatcatg cccgtattcc ctgccagctt taacacgtcc ttcctgtgct gcatcatcgc
     4081 ctctttcacc ccttcccggt gtttttccag cgatttctct gtcgagactg tgaacagggc
     4141 tatagcgtct ctgattttcg tctcgtttga tgtcacagcc tcgctgacag actcgccgag
     4201 cttcctgaac tcgtcgttca gcattttctc tgtagattcg gctctctctt tcagcttttt
     4261 ctcgaactcc gcgcccgtct gtaaaagatt gctcataaaa tgctcctttc agcctgatat
     4321 tccttccgcc gttcgggtct gcaatgctga tactgcttcg cgtcaccctg acaacctcaa
     4381 ctcccacatc tgtgagtgcc tgaatcacat cctgacggtc ttttatctct ccggtacggt
     4441 aaagcacatc aagcccccgt gtaacgctct cagcaatcgc ctgtttcgtt tccggcaggt
     4501 tatcagggag tgtcagcgtc ctgcggttct ccggggcatt cgggtcatgc agcccgtaat
     4561 ggtgattcac cagcgtctgc caggcatcaa ttctcggcct gtctgcgcgg tcatagtacg
     4621 gctggaggcg ttttccgctc tgtagctcca tgttcggaat gacaaaattc agctcaagcc
     4681 gtcccttgtc ctggtgctca acccacagga tgctgtactg atttttttcg agaccgggca
     4741 tcagtacacg ctcaaagctc gccatcactt tttcacgtcc tcccggcggc agttccttct
     4801 ccgcgaacga cagaacacct gacgtgtatt tcttcgcaaa tggcgtggca tcgatgagtt
     4861 cccggacttc ttccggatta ccctgaagca ccgttgcccc ttcccggtta cgctcccttc
     4921 ccagcaggta atcaaccgga ccactgccac cgccttttcc cctggcatga aatttaacta
     4981 tcatcccgcg ctccctgttc cctgacgacc tgccgtaagc tgcgcaactc tctctcgatg
     5041 gccatcagtg cggccaccac ctgaacccgg tcaccggaag accactgccc gctgttcacc
     5101 ttcctcgccg tctgattcag gttattcccg atggcagcca gctgacgcag taacggcggt
     5161 gccagtgtcg gcagcttccc ggaacgggca accggctcac ccaggcagac ccgccgcatc
     5221 cataccgcca gttgtttacc ctcacagcgt tccagtaacc gtgcatgttc atcatcagta
     5281 acccgtatcg tgagcatcct ctcgcgtttc atcggtatca ttaccccatg aacagaaata
     5341 cccccttaca cggaggcatc agtgactaaa caggaaaaaa ccgcccttaa catggcccgc
     5401 tttatcagaa gccagacatt aacgctgctg gagaagctca acgaactgga cgcagatgaa
     5461 caggcagaca tctgtgaatc gcttcacgag cacgccgatg agctttaccg cagctgcctc
     5521 tcacgcttcg gggatgacgg tgaaaacctc tgacacatgt agctcccgga gacggtcaca
     5581 gcttgcctgt gagcggatgt cgggagcaga caagcccgtc agggcgcgtc agcaggtttt
     5641 agcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc
     5701 ttaaccatgc ggcatcagtg cagattgtaa gaaaagagcg ctatatgcgg tgtgaaatgc
     5761 cgcacagatg cgtaaggaga aaatgcacgt tccggcgctc ttccgcttcc tcgctcactg
     5821 actcgctacg ctcggtcgtt cggctgcggc gagcggtgcc ggctcactca aaggcggtaa
     5881 tacggttatc c
//
