LOCUS       NZ_CP085782             2495 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 48411CZ plasmid p48411CZ_Col,
            complete sequence.
ACCESSION   NZ_CP085782
VERSION     NZ_CP085782.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377411
            Assembly: GCF_020695725.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 822x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695725.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:42:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,851
            CDSs (total)                      :: 4,734
            Genes (coding)                    :: 4,643
            CDSs (with protein)               :: 4,643
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 91
            CDSs (without protein)            :: 91
            Pseudo Genes (ambiguous residues) :: 0 of 91
            Pseudo Genes (frameshifted)       :: 33 of 91
            Pseudo Genes (incomplete)         :: 54 of 91
            Pseudo Genes (internal stop)      :: 19 of 91
            Pseudo Genes (multiple problems)  :: 15 of 91
            Pseudo Genes (short protein)      :: 1 of 91
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085782.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="48411CZ"
                     /isolation_source="bile"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p48411CZ_Col"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2019-01"
                     /collected_by="Fakultni nemocnice Bulovka"
     gene            complement(214..399)
                     /locus_tag="LJU35_RS23315"
                     /old_locus_tag="LJU35_23315"
     CDS             complement(214..399)
                     /locus_tag="LJU35_RS23315"
                     /old_locus_tag="LJU35_23315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(765..992)
                     /locus_tag="LJU35_RS23320"
                     /old_locus_tag="LJU35_23320"
     CDS             complement(765..992)
                     /locus_tag="LJU35_RS23320"
                     /old_locus_tag="LJU35_23320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            2200..2418
                     /locus_tag="LJU35_RS23325"
                     /old_locus_tag="LJU35_23325"
     CDS             2200..2418
                     /locus_tag="LJU35_RS23325"
                     /old_locus_tag="LJU35_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          80..167
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1408..1936)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ggacgagtga gagtaatcta atctaacttg attgagttcc gggcgtgccg cccggttttt
       61 tttgctgttt cccggtgtcc gttccgggac aggatgtgca tttgtaatac cgcgcacgcg
      121 ccggcatcac aaataccctg gtcagggctg cgccccgaca ccccgccatc gcctcacggc
      181 cgcgcactga attgcgcacc cgttcaccga ttttcacgca ttttcctcgc ctatctgtgc
      241 gttcagcatg gcgtaaagcg cttcggcctg atcgtgcagg tgctcacagc agccggcagc
      301 ctcatccaga tccatctgct cgagccgctc aaggaggaga agtgtctgtg cgcggataaa
      361 tttggccata ttgagggcgg cggattcctg tctggtcact ggtgattttc cccgtctggt
      421 gatgattttt gcgtgaaaag agcatggcgt cattctggtg catagtcatg catgccgtta
      481 aaatttaaca ggcgcgtttc tgacgggttc cggggtggtt tgttgtggtt tttgtcatgg
      541 tttcgtcagg aacgcgctga acgcgtctca ggcggtgcgg gcaacggatg ttatggtaaa
      601 ttcttggggg cttgattata aaataccgca cttcctctta tgtcttgtgc tttttagaca
      661 accctgaaag cttttagctt tggtgatgca atttgctggg attttggcgg agaaccacag
      721 gtaaagaaaa aggccacatt agcggccttt ttcggagaag atgctcagtg cggaatttcg
      781 gagacttctt taacgtcggt tttattgatc tgctgtttga caccattcgc gtctgtatag
      841 ccaagcaggc ctgagtctga ttctttaggt ttgccgtcgc tgacaatagt gcgaccgtca
      901 ttggtgtgta tggcatagct tgtgcgcgtg cagccggtga gggctgaaag cgcaactgcc
      961 gctgcgaaca ctgaaacaaa aatctttttc aaagccagct ccttttatcc atatcagttt
     1021 tggtcactaa ccatctaact ataggtcatt ttttatgtga aaagaggctt gatggtgggc
     1081 tgtatatcga gttaggcgcg aggtgctatg gtcaaagtgt ggtgtcaggc ggggaggagg
     1141 cttatatgag atgtaatccg gccgatacat ataccaagat gatttaagta ttcctctttg
     1201 tgaggttgga tgtctaattt ataaaggatc ttcttgagat cctttttttc cgcgcgtaat
     1261 ctcttgccct gtaaacgaaa aaaccaccct ggcaggtggt ttttcgaagg ttaggtaatc
     1321 ctggcagatc ccctaaccgt ggcaacagtc ttgtgcgaga catgtcacca aatttgtcct
     1381 ttcagtgtag cctcactaag gccgccactt caagaactct tgagacatct ctcgcacatc
     1441 ctgtttgcca atggccgttg ccaatggcga ttagtcgtgt ctttccgggt tggactcaag
     1501 ttgatagtta ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc
     1561 catcctggag cgaactgcct tcccggaact gagtgtcagg cgtggaatga gaaaccgcgg
     1621 ccataacagc ggagtgacac cggtaaaccg aaaggcagga atgcggggga gcacgaggga
     1681 gccaccaggg ggaaacgcct ggtatcttta agccgcatcg ggtttcgcca ccactgattt
     1741 gagcgtcaga ttctgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttggc
     1801 gcggccttat gctttcttcg ttaagtatct tcctggcatc ccccaggaaa tttctgatcc
     1861 atccgtaagc ccgtcccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg
     1921 aggaagcgga atatattctg tatcacattt tctcctgacg cgttttcttt cactttctgc
     1981 gcctgtctta tgtggcatta atgctatgtg ttactgccat gctacatctt aagccagtat
     2041 acactccgct agtgctccgt gactggtccg gcgctgcgcc cggaacccgc ctgtaccggt
     2101 tcagcagccg ttccggcctg actgcaattt tttttttttc atccctgccc gctaccctgt
     2161 aaacctttct tctgcgttgc cgttaacagt aggtaagcca tgtttggttt ttcttccgga
     2221 gccctgatga ctcttagtga tgatgagcga cacctgcttg tgtccgttgt ttcggtctgg
     2281 ctacggcgtg ccggagggga tgccggagcc atgatgctgg atgcataccg gcagattttg
     2341 tctgaaaccg aaccggccgt aaggactgtc atgctcgaat ttcttgaatc cgttcgcatt
     2401 cattacattt cgtcctgagc attttctgta tttctgtcca tacttaaacg aggcggccgt
     2461 cacggacggt ggtcgtcgtt ccatgatgct gttat
//
