LOCUS       NZ_CP085777             3861 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 48947CZ plasmid p48947CZ_3, complete
            sequence.
ACCESSION   NZ_CP085777
VERSION     NZ_CP085777.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377412
            Assembly: GCF_020695705.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 888x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695705.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:03:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,037
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 37 of 119
            Pseudo Genes (incomplete)         :: 82 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 18 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085777.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="48947CZ"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p48947CZ_3"
                     /geo_loc_name="Czech Republic: South Bohemian Region"
                     /lat_lon="48.97 N 14.48 E"
                     /collection_date="2019-02"
                     /collected_by="Nemocnice Ceske Budejovice"
     gene            complement(18..1421)
                     /locus_tag="LJU36_RS23590"
                     /old_locus_tag="LJU36_23590"
     CDS             complement(18..1421)
                     /locus_tag="LJU36_RS23590"
                     /old_locus_tag="LJU36_23590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023280855.1"
                     /translation="MNEKSIDEILAERQLKEFNESKFGQFFNNQKLKEQELTYLNNKAL
                     DIVNEEDLFEIMSLIPQPFITEENKNVDVLSLIYNNFLENTEIPLTLPIFSFLSFLSAF
                     CVKNEITYTIPLSDEKSPLDTWITILAPSGSAKTFSNSQIKKVIPVGIDGKKVIESNFV
                     KPNGPAKFIEELSNLPETKNGENQYGFWIEDEVSQMFKQIEQIGSPLSEIKEYLLKSYD
                     HEKLTRKSKKETIETKNIVLTLLFINTFESYANNISNESMTDGLMRRFNLIYTEKDSER
                     DFTDYSLYNLKKIRDETLQDKISELFNSIKPDQNFTFSDNCFKLYQKYFKIFWHRKYKT
                     ILPEKENFYRTYMMQSWKFAVFYHLILKREGTEIQGDCLVFGMKISLLFANSMKKFFDF
                     KLNKKLTPKKKEKIKVDFTKYIEYLNENTDITLRTFCRKFNLKKADSISILKSLECNNY
                     QHKLIEELE"
                     /name="hypothetical protein"
     gene            complement(1850..3274)
                     /locus_tag="LJU36_RS23595"
                     /old_locus_tag="LJU36_23595"
     CDS             complement(1850..3274)
                     /locus_tag="LJU36_RS23595"
                     /old_locus_tag="LJU36_23595"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_157916217.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTNKPEEQQFVEVLSQTNRSDI
                     ENFNEYIKEKNFKNPFITGVLSFEEKDIDPKLKTKIMNEFEEMMFEGIEPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVKEHK
                     ERNTLVNTLNNKIPADKKAIVEKLQEDILSKIITEEINSREELISFIQSKNIIINRVRE
                     NAISIKFDKADKPISLKGDIYEQNRDYKTYRTESTIDHRADQQYVENQLRNHRENFDTE
                     LKKRHSRNRTRFKPTQSKDQKNVEFRVKKANDSKNVRSEISDNNNVPFRNEPNIDFFHN
                     NKTMEDVSNEQNFTNDKTKPREEEPAKFDLSREIETVNTQQQFIRSRVEEAGRNQQQFR
                     KSLDRTQERSRVCRRSLHSIISNIRRYVRTLSIHTRQNERRQKIEHRQKIFLEKLKKQQ
                     TQNNQRSVVRSRFKPH"
                     /name="hypothetical protein"
     gene            complement(3264..3608)
                     /gene="mobC"
                     /locus_tag="LJU36_RS23600"
                     /old_locus_tag="LJU36_23600"
     CDS             complement(3264..3608)
                     /gene="mobC"
                     /locus_tag="LJU36_RS23600"
                     /old_locus_tag="LJU36_23600"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_032415722.1"
                     /translation="MSYRERYQRKNFVSLCLNDEELSELENIAERLNMKRAAAAREILF
                     TNSKRLKSQIKKNDNSEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFALLIEEIF
                     EDMEKLKNDT"
                     /name="mobC"
ORIGIN
        1 taaaattcgc ttgttaatta ctccagttct tcaataagtt tgtgttgata attattacat
       61 tcaagacttt tcaaaattga tattgaatca gcttttttca ggttgaattt tctgcaaaat
      121 gttctcaatg taatatctgt attttcattt aaatattcaa tgtattttgt gaaatctact
      181 tttatttttt cttttttctt tggtgttagt tttttattta gtttgaaatc aaaaaacttt
      241 ttcatactat tggcaaatag tagagatatt ttcataccaa acactaaaca atcaccctga
      301 atttctgttc cttctctttt taaaataaga tgataaaaca ctgcgaattt ccaactttgc
      361 atcatgtaag ttctgtaaaa gttttctttc tctggtagaa tggttttata ttttctatgc
      421 caaaaaatct tgaaatattt ttgatataat ttaaagcaat tgtctgaaaa tgtgaaattt
      481 tgatctggtt ttattgagtt gaataattca gatattttgt cctgtaatgt ttcatctctt
      541 atttttttca aattatataa tgagtaatct gtgaaatctc tttcactgtc tttttctgta
      601 taaatgagat taaatcttct cattaaacca tctgtcattg attcatttga aatgttattc
      661 gcataacttt caaatgtatt tataaataac agtgttaaaa ctatattttt agtttctatt
      721 gtttcttttt tgcttttcct tgttaatttt tcgtggtcat atgactttaa caaatattct
      781 ttaatttcag atagcggaga tccgatttgt tctatctgtt taaacatttg tgaaacttca
      841 tcttcaatcc aaaaaccata ttgattttcg ccgtttttgg tttcgggaag atttgaaagt
      901 tcttcgataa atttcgcagg gccattaggt tttacgaaat ttgactcaat aacttttttt
      961 ccgtcaatac ctactggaat aacctttttt atttgtgaat ttgaaaaagt tttcgcactt
     1021 ccagaaggtg ctaaaattgt gatccaagtg tcaagcggcg atttttcgtc gcttaatgga
     1081 attgtatatg tgatttcatt tttaacacaa aaagcactta aaaaacttaa aaaagaaaaa
     1141 ataggaagtg ttaatggtat ttctgtattt tctaaaaagt tattatatat aaggcttaaa
     1201 acatcaacat ttttattttc ttctgtaata aatggttgag gtattaatga cattatttcg
     1261 aataaatctt cttcattcac tatgtctaat gctttattgt taaggtatgt tagttcttgc
     1321 tcttttagtt tttgattatt gaaaaactga ccaaatttcg attcgttaaa ttcttttagt
     1381 tgtctttcag ctaatatttc atcaattgat ttttcattca taattaattt ctcctatttt
     1441 ggttatatta catatataac atatgaaaaa acattgtcaa cggtttttgt aaaaaaccta
     1501 tgacaggttt taaaccttga tttatgacac tatgacacta tgacacaaag ggcaaagcct
     1561 tgatatgtaa ggatttaacc actttttatg gttatgacaa aacctatgac aaaagttatg
     1621 acacttgtct taaaatcaca attgagaaaa aaagctgttt tttatgtcat aagaaaatga
     1681 aaaatattca gacaggttct tgtcaaaaac tccgacaaag ttatgacaga aaaaccgccc
     1741 tgaccgggca ggccaacccc tcacaagcaa aaaccagcct ctggctggtt ctcacttgtt
     1801 cacggttaag gttttatcgt agtgggttag aagagtgttc agaacggttc taatgcggtt
     1861 taaatcgact tctgacgaca cttctttgat tgttttgtgt ttgctgtttt ttgagctttt
     1921 caaggaagat tttttgtctg tgttcgattt tttgtcttct ctcgttctgt cttgtatgga
     1981 ttgaaagcgt tctgacatat cttctaatat tagaaataat tgaatgtaga gatcttctac
     2041 atactctaga tcgttcttga gttctatcaa gcgactttct gaattgctgt tgatttcttc
     2101 cagcttcttc aactcttgaa cgaataaatt gttgttgtgt attaactgtt tcaatttctc
     2161 ttgatagatc aaattttgct ggttcttctt ctcttggttt tgttttatca tttgtgaaat
     2221 tttgctcatt gcttacatcc tccatagtct tattattatg aaaaaaatca atattaggtt
     2281 cattcctaaa aggaacatta ttattgtctg atatttcaga tctaacattt ttactgtcat
     2341 ttgctttttt aactctgaac tcaacatttt tctggtcttt gctctgtgtt ggcttaaaac
     2401 gagttctatt tctgctgtgt ctttttttaa gctctgtgtc aaaattttct ctatggtttc
     2461 tgagttggtt ttcgacatac tgctgatctg ctctgtgatc gattgttgat tctgttctat
     2521 aagttttgta atctctgttc tgttcatata tatctccttt taagctgata ggcttatcag
     2581 ccttatcaaa ttttattgat atagcatttt ctctaactct atttattatt atgtttttag
     2641 attgaatgaa tgatattaat tcttctctgc tgtttatttc ttctgtgatt attttcgata
     2701 aaatatcttc ttgtaatttt tccacaatag cttttttatc tgctggtatt ttattattta
     2761 aagtatttac aagtgtgttt ctttctttgt gttctttaac atctaaaaca gaactgatat
     2821 tattttcata atttattttt tcgcaaaatg agttaaataa ttttttatca gataaatgat
     2881 aataacattg aaaatgtttt ttattcatta aagaattaaa agttgtatag tttaattcaa
     2941 gtctgttttt atctttgtgt tgtatccaga tcactggtgg gcggttttca ggttctattc
     3001 cttcaaacat catttcttca aattcattca ttatttttgt ttttaatttt ggatcgatgt
     3061 ctttttcttc aaaagataaa acaccagtta tgaaagggtt tttgaagttt ttttctttta
     3121 tgtattcgtt gaaattttca atatcagatc tgttcgtttg agataaaact tcaacaaatt
     3181 gttgttcttc tggtttgtta gttcttaata aataatcgag tgagttttta acacttcgct
     3241 gatttttctt tcctccgata tgtttatgta tcatttttta atttctccat atcttcgaat
     3301 atttcctcaa ttaacaatgc aaattcttca ccgttcccag ataaaaattt atcaagattt
     3361 gtattcattt tttttgcgat ctggtttatg ttgtttgaga ttttagaata taaaaacaag
     3421 atttcagaat tatcattttt ctttatttgt gattttaatc tctttgagtt cgtgaataat
     3481 atttcacgag ctgctgctgc tcgtttcata tttaatcttt cagcgatatt ttcaagctct
     3541 gataattctt catcattcaa acacaatgag acaaaatttt ttctttggta tcgttctctg
     3601 taactcaaat ttaactcctt tgggtttaag gttttgattt taaggggatt gaagggggaa
     3661 cccctcacaa gtaaagtaat acgaagtatt actctaactt gctatctatt acatagataa
     3721 caaattaaaa ccttaagtca agggcaaaac tgaatataat ttgacctttt ataaaaccgt
     3781 gagaaaataa aaaagcccgt cagggcttta ttatttttga ggggttgaac aagcgaaaat
     3841 atatttgaat atggcacaat t
//
