LOCUS       NZ_CP085776             4037 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 48947CZ plasmid p48947CZ_2, complete
            sequence.
ACCESSION   NZ_CP085776
VERSION     NZ_CP085776.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377412
            Assembly: GCF_020695705.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 888x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695705.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:03:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,037
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 37 of 119
            Pseudo Genes (incomplete)         :: 82 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 18 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085776.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="48947CZ"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p48947CZ_2"
                     /geo_loc_name="Czech Republic: South Bohemian Region"
                     /lat_lon="48.97 N 14.48 E"
                     /collection_date="2019-02"
                     /collected_by="Nemocnice Ceske Budejovice"
     gene            548..1807
                     /gene="mobQ"
                     /locus_tag="LJU36_RS23565"
                     /old_locus_tag="LJU36_23565"
     CDS             548..1807
                     /gene="mobQ"
                     /locus_tag="LJU36_RS23565"
                     /old_locus_tag="LJU36_23565"
                     /inference="COORDINATES: protein motif:HMM:NF041496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_227803259.1"
                     /translation="MALYRLEMQNVSRANSVSSVAKAAYRHRSVMIDERTGEMHGEKSA
                     NRDDLVYAEILAPKGTPDFLTKSSNELWNFVEKTEKRKDARTAKEFKITLPCELTNEQN
                     IKLLKEYLTKNFVSKGIICDFVIHNDKDNKNPHAHVMITTREITPQGFGKKVRSWDEEK
                     TLHDWRKQWAKVQNQYLKNAGLKSRVSHRTLEEQKNIMIDLAKKAEDAKDFDKSILYLA
                     KAIELDRPPMQNLSRKKWRTKEGQEQRKRDQAIRDKAREDARAVRVLRGVAVVDVASFV
                     VHPLEKKEQSKNNDFSEKKSWWRSTVNFIKNIKAKFSTKKDIKPVQEKEPDLMIDPVTG
                     LPITKQKWQEMSDRASQKAPEAAISYKVRQSDGEELKTAQNALQDVLDVSEGKSVKVKS
                     VQVPGSRRRRVIDQDSSDNG"
                     /name="mobQ"
     gene            2124..2981
                     /locus_tag="LJU36_RS23570"
                     /old_locus_tag="LJU36_23570"
     CDS             2124..2981
                     /locus_tag="LJU36_RS23570"
                     /old_locus_tag="LJU36_23570"
                     /inference="COORDINATES: protein motif:HMM:NF015076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_053514985.1"
                     /translation="MILQKEYSRSDFINQIISRPFCTDDFKRDGIYRTNKRRALNSVYI
                     EHNNDSFINSIVFDIDSDTAAIAWQDANIPKPNFITQNPANGHAHLFYALSSPVCITEN
                     ARRKPQKLLKGVIEGLTERLGADPCYTGKITKNPLNPRWRTFWNEQPRFELNYLCEFID
                     TNKRVKKEVRKSIVAEGRNTALFDSLRFYAYSIIFKYQKNDDFCGFMSALEEEAEHIND
                     SFEDQLGFKEINHTIRSISSWVWGTFDSDTFSEIQSNRAKKAKGKIKSKTKNKLSRILM
                     INEE"
                     /name="replication initiation protein"
     gene            2986..3258
                     /locus_tag="LJU36_RS23575"
                     /old_locus_tag="LJU36_23575"
     CDS             2986..3258
                     /locus_tag="LJU36_RS23575"
                     /old_locus_tag="LJU36_23575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0003700 - DNA-binding transcription factor activity
                     [Evidence IEA]; GO:0016987 - sigma factor activity
                     [Evidence IEA]"
                     /GO_process="GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]; GO:0006355 - regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="WP_072159717.1"
                     /translation="MAALEQTKRKKPAKYFAEKMGVSVRTVKRYIAQPRKDYESEAENR
                     RKTAYTLHHEKGLKWREVAEKMNTTEHAVKALAKRYKQIDQIERG"
                     /name="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(3274..3549)
                     /locus_tag="LJU36_RS23580"
                     /old_locus_tag="LJU36_23580"
     CDS             complement(3274..3549)
                     /locus_tag="LJU36_RS23580"
                     /old_locus_tag="LJU36_23580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_053514984.1"
                     /translation="MSQKKEQKTAEKPVAGPLRKHRTRTEKIDDLKKQISDLRKAEREE
                     RQRARNEILLAVGKAVYEKAGKPKNIYGLQQFVESEEFKVDFKNDF"
                     /name="hypothetical protein"
     gene            complement(3682..3960)
                     /gene="traD"
                     /locus_tag="LJU36_RS23585"
                     /old_locus_tag="LJU36_23585"
     CDS             complement(3682..3960)
                     /gene="traD"
                     /locus_tag="LJU36_RS23585"
                     /old_locus_tag="LJU36_23585"
                     /inference="COORDINATES: protein motif:HMM:NF018152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_172750907.1"
                     /translation="MGSKKTLEQRMALAQDRLSKLKKEASEKERKAREHIKFKAGGEVT
                     RVFGLDYDEAVLLGFLAVYDKLSEDQKRQIKKDGENIIKSRLENAEK"
                     /name="traD"
     Typing          639..1804
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.746"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaaataaca ttttcagctc atagatattt atgtaattat ttagtgttaa ataaacaaga
       61 tcataatcag ttataatttg ttcatcatgg tctttatttt caagatcttt atttatcaaa
      121 tatatcaacg cttcaaacaa tgatctttta ttgttataga ttgcattgtc gatcgcgtaa
      181 tacagcttta aatacccagt aaaagcatct tcattaactt cgtcaatctt ttttctattt
      241 cttttttatt cattttatca ccaaaatata gattatattt tactcaatat cgtaactttt
      301 aaatattgtc aaatttgact ttaatttaat cagagttaat ttatatgtat cagagaaaga
      361 aaaactatat aggttttcat tcctctttta gtttgagcgt cagcgaaaaa ataaaagata
      421 aggaatgccc ggttacgcat tgtaaacaat gccggggtct gccgacggct aaaacctgtt
      481 tttcttacga aaaactaaaa ccaaaaccca aaacaaaaaa taggggttct catgaggata
      541 taattatatg gctttatata ggttagaaat gcagaacgta agccgtgcaa atagtgtcag
      601 ctcagttgca aaggcagctt atcgtcatcg ttcagtcatg atagatgaac gaacaggaga
      661 aatgcacggc gagaaatcag ctaatcgtga cgatctcgtt tatgctgaaa tattagcacc
      721 aaaaggcacg ccggattttc ttactaaatc gagcaatgag ctatggaact ttgtcgaaaa
      781 aacagagaag agaaaagacg caagaacagc aaaagaattt aaaataactt tgccttgtga
      841 acttaccaat gaacaaaata taaaactatt gaaagaatac ttaacaaaaa actttgttag
      901 taaaggcatt atttgcgact ttgttataca taacgataaa gacaacaaaa acccacacgc
      961 tcacgttatg attacgacaa gagaaataac cccgcaaggt tttggtaaaa aagtaagatc
     1021 atgggatgaa gaaaagacac ttcatgattg gcgtaaacaa tgggcaaaag ttcaaaatca
     1081 gtatttgaaa aatgcaggct taaaatctcg cgtcagtcat agaacactag aagaacaaaa
     1141 aaatataatg atcgatttag ctaaaaaagc agaagatgca aaagattttg acaagtctat
     1201 tttgtattta gcaaaagcta ttgaactaga tcgacctcct atgcagaacc tatcacgtaa
     1261 aaaatggagg acaaaagaag gtcaggaaca acgcaaacgt gaccaagcga tcagagacaa
     1321 ggcaagagaa gatgcaaggg ccgtcagggt gttgaggggt gttgcagttg ttgatgttgc
     1381 ttcattcgtg gttcatccgt tagagaaaaa agagcagtca aaaaataatg acttttcaga
     1441 aaaaaaatct tggtggcgtt cgactgttaa ttttataaaa aatattaaag ctaagttctc
     1501 aactaaaaaa gatattaaac ccgttcaaga aaaagaacct gatttaatga ttgatccggt
     1561 cactggttta ccgattacaa aacagaaatg gcaagaaatg agtgacagag cttcacagaa
     1621 agcaccagaa gctgcgattt cgtacaaggt aaggcaatcg gacggggaag agctaaaaac
     1681 ggctcagaat gcgttacaag acgttttaga cgtatcagag ggtaaatcgg tcaaggtgaa
     1741 gtctgtgcag gttccgggtt ctcgtaggcg tcgagtgata gaccaagaca gcagtgacaa
     1801 cggttagcta accagcacag ccagatcctg cataagatta cagggcttcg ctcatagcct
     1861 ccggccttat gggttcaggc tatagcttaa cttgtaatga tttgaagcct tcggcctgtg
     1921 cttcgcaaaa atacccccca aacccattat tacctgatat ggctaatcgt ccctcttgac
     1981 accttttttt ggtgacactc ttgacaccat gaaaggttct gttagaacag taatataaaa
     2041 tataaaaaca aaaatccctg atttccgaat gtttggcgac aggagaaaat cagggatcta
     2101 caataaaagg attgtattaa aaaatgatac tacaaaaaga atatagcaga agtgatttta
     2161 taaatcaaat tatatcaaga cctttctgca ctgacgactt caaaagagac ggtatttata
     2221 gaacaaacaa aagacgagct ttaaacagcg tttatattga acacaacaac gatagcttta
     2281 ttaactctat tgtgtttgat attgacagcg atacagctgc tattgcatgg caagatgcaa
     2341 atataccgaa acctaacttt ataactcaaa atccggctaa tggtcacgct catttgtttt
     2401 atgctttatc gtctcccgtt tgtataacag aaaacgccag aagaaagccg caaaagttat
     2461 taaaaggcgt tatagaagga ctcacggaaa ggttaggtgc agatccatgt tatacgggca
     2521 agataacaaa aaaccctctc aatccgagat ggcgtacatt ttggaacgaa caaccaagat
     2581 ttgaattgaa ctatctatgt gagttcatag atacaaataa aagagtaaaa aaagaagtaa
     2641 gaaaatcaat agttgcagag ggaagaaata cagcgttatt tgatagtctg cgtttttacg
     2701 cttacagcat aatttttaaa tatcaaaaaa atgacgattt ttgcggtttc atgtcagcat
     2761 tagaagaaga agctgaacac ataaatgatt catttgaaga tcaactaggc tttaaagaaa
     2821 taaatcacac aataagaagc atttcttctt gggtgtgggg gacttttgat agtgatactt
     2881 tcagcgaaat acaaagcaac agagcaaaga aagctaaagg aaaaataaaa tcaaaaacaa
     2941 aaaataaact atctagaatt ttaatgatta atgaggagta gaaaaatggc agcattagaa
     3001 caaacaaaaa gaaaaaaacc tgcgaaatat ttcgctgaaa aaatgggcgt tagtgttaga
     3061 acagttaaaa gatatattgc acaaccaaga aaagattatg aatcagaagc tgaaaacaga
     3121 agaaaaacag cttatacatt acatcatgaa aaaggtttaa aatggcgtga agtagctgaa
     3181 aaaatgaata caacagaaca tgcagttaaa gcattagcca aaagatataa acaaatagac
     3241 caaatcgaaa ggggctagga tgccccttca tggttagaaa tcgtttttaa agtctacttt
     3301 aaattcttct gactctacaa actgctgcaa gccataaata ttctttggct ttcctgcttt
     3361 ttcgtaaaca gctttaccaa cagccaacaa tatttcattt ctagctcttt ggcgttcttc
     3421 tctttctgct tttctcaggt cactaatctg tttcttcaaa tcgtctattt tttcagttct
     3481 agttctgtgt tttctcagtg gcccagcgac tggtttttct gctgtttttt gctctttttt
     3541 ttgagacatt tttcactctc ccgtatgata tgttttttaa acataccaca acgccgggct
     3601 atcgtgaaga ctaacgcaca agccccgcca gcggcgggtt cttgcttgtt caacccttcc
     3661 aagtgtcaga ttggaaatct gtcacttttc agcgttttct agacgtgatt ttatgatgtt
     3721 ttcaccgtct ttttttatct gtcttttttg gtcttctgat agcttgtcat aaacagctaa
     3781 aaaacccaac aaaacggctt cgtcataatc taaaccaaac accctagtaa cttctccgcc
     3841 agctttaaat tttatatgct cccttgcttt cctttccttc tctgacgctt cttttttaag
     3901 tttagataat ctatcttgtg caagtgccat tctttgttct aacgtttttt tacttcccat
     3961 tttcaacctc tctttttatt ttttctatct ctgaaatcat acttttaaaa tcaaaatcat
     4021 tgatatttct ttttaat
//
