LOCUS       NZ_CP085775             4416 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 48947CZ plasmid p48947CZ_1, complete
            sequence.
ACCESSION   NZ_CP085775
VERSION     NZ_CP085775.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377412
            Assembly: GCF_020695705.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 888x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695705.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:03:04
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,153
            CDSs (total)                      :: 5,037
            Genes (coding)                    :: 4,918
            CDSs (with protein)               :: 4,918
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 37 of 119
            Pseudo Genes (incomplete)         :: 82 of 119
            Pseudo Genes (internal stop)      :: 19 of 119
            Pseudo Genes (multiple problems)  :: 18 of 119
            Pseudo Genes (short protein)      :: 1 of 119
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085775.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="48947CZ"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p48947CZ_1"
                     /geo_loc_name="Czech Republic: South Bohemian Region"
                     /lat_lon="48.97 N 14.48 E"
                     /collection_date="2019-02"
                     /collected_by="Nemocnice Ceske Budejovice"
     gene            complement(join(3851..4416,1..301))
                     /locus_tag="LJU36_RS23535"
                     /old_locus_tag="LJU36_23535"
     CDS             complement(join(3851..4416,1..301))
                     /locus_tag="LJU36_RS23535"
                     /old_locus_tag="LJU36_23535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_063617447.1"
                     /translation="MFNIENNREILKSQIPLKPYATNDLDFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMIFDLDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPLHSHWSPFWSGADLYELNDLADCFDDL
                     EDPKKRENTELAFGRNVEMFDTQRQWAYKNVLKYKNESSFDAFYKELLLKCETFNSFAN
                     YNNLLPYKELVSTAKSIAKFCWKEFSNEKLHDIQSKKQKARRSKRTEKQKLHTKTLLEK
                     AIMINEE"
                     /name="replication initiation protein"
     gene            602..817
                     /locus_tag="LJU36_RS23540"
                     /old_locus_tag="LJU36_23540"
     CDS             602..817
                     /locus_tag="LJU36_RS23540"
                     /old_locus_tag="LJU36_23540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001171587.1"
                     /translation="MQKPKRRLGFMVGKSNATLPPDDETEAELDQEVLKLFYPNQETQD
                     TIERSEAGTDVFMASGSDDLFKKLKL"
                     /name="hypothetical protein"
     gene            1464..1724
                     /locus_tag="LJU36_RS23545"
                     /old_locus_tag="LJU36_23545"
     CDS             1464..1724
                     /locus_tag="LJU36_RS23545"
                     /old_locus_tag="LJU36_23545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063617446.1"
                     /translation="MNENDIDQSLLELLARYFYYIDPNEENTAFLEANKDEQDLCYFVA
                     YRYIKENKTQDLIDALKDQKDEDYIKAMKDYVYGGGKYGYR"
                     /name="hypothetical protein"
     gene            1711..2082
                     /gene="mobC"
                     /locus_tag="LJU36_RS23550"
                     /old_locus_tag="LJU36_23550"
     CDS             1711..2082
                     /gene="mobC"
                     /locus_tag="LJU36_RS23550"
                     /old_locus_tag="LJU36_23550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_063617445.1"
                     /translation="MDTDRKRGRIGLNHEVRRDISIQFRVNKAEFDYLKELVEASGLER
                     GHYIRKEILNSEPVIKRKMPEFEQSLYREIQHQGNNINQIAKKLNTNEDFKQSLAAEAN
                     FAIKQLMKRILKACEVYTV"
                     /name="mobC"
     gene            2079..3563
                     /locus_tag="LJU36_RS23555"
                     /old_locus_tag="LJU36_23555"
     CDS             2079..3563
                     /locus_tag="LJU36_RS23555"
                     /old_locus_tag="LJU36_23555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_063923475.1"
                     /translation="MKGSVAKTGKSFKNRVDYILKDDHSFICSNMASDYNNVSDLTDEF
                     KTVSSFRQDIKKPVFHAFLSLPKNEHLTDQQWEEIAKDYLKEMNIDIEKHQYICVRHND
                     TDKEHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITQSLKGQKSDVSAPT
                     KNEIEQALRTGEKPARIVLQNALQAAMVGKPDLQTFIDRVQAVGIEPRFNVASTGNVAG
                     VSFSVGDVAFKGSQLGKKYSWNTIKNKVIYDKNRDDELVRSFSARKDDEPNSIRRSLAE
                     PNINDSGAVDRIDDDIIRISQHSIKSDTTDTYESKQLVRSNNHSFKLSEKYNRPASHKV
                     RRTTQKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSSSISRILDHAETANAATNN
                     FRLKGRGNINSRATKIEEIRERLGIKKPIIQPKNKEKIVKEEIKKKPLGWSMNENRFKS
                     KLKEALKKEKPKNETTPTQLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3572..3847)
                     /locus_tag="LJU36_RS23560"
                     /old_locus_tag="LJU36_23560"
     CDS             complement(3572..3847)
                     /locus_tag="LJU36_RS23560"
                     /old_locus_tag="LJU36_23560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="WP_000125721.1"
                     /translation="MTALHTVKRNKTAKEMAQRLNISDRTVRKLIALPREDYEKEAKQR
                     RLKAFVLREAGFKWSEVGERLGTTKHGAIALYKRYQQIDAPTTKEA"
                     /name="HTH domain-containing protein"
ORIGIN
        1 ctgttgatga gatcggagca ttaagcatat aaccgtaatg acatctacca gagtctggtg
       61 attttgttat aaaagacggg atgggcaaat gagcattttc aaactcataa aagcagtttt
      121 ctttgtctaa atcaaatatc attgtatgaa gatacaacgg gtgatttact tgtaaataaa
      181 gcatatctaa agctttattc ttattttgaa tagtcaatcc aaaatcaaga tcattagtag
      241 catatggctt taaaggtatt tgacttttta atatctctct gttattttct atattgaaca
      301 tttagcagta tcctttctgt aatgccccgt tctctcactg tcgccaaact aatcagagaa
      361 cgcgggcttt tcttttattt ataatctctt acagttttac tataccaaaa aaacttgtca
      421 atagatccgt caaaaaaagt tgttaagtag gaagtctacc aatatcaggt attagacgga
      481 gttttgggga cactacacca cagcgaagca caggccgaag gctatggcag agtaagtcac
      541 actgtaacgt atactaaacg tcatatgcag gatctggctg tgctggatag gagaacatga
      601 gatgcagaaa ccaaagcgta gactcggttt tatggttggt aagagcaatg caacattgcc
      661 accagatgac gaaacagaag cagagcttga tcaggaagtc ttgaagctgt tttatcctaa
      721 ccaagagaca caagacacta tagaacgctc agaagccggt acagacgttt tcatggcctc
      781 cggttctgat gacctgttca aaaaactgaa gctgtaggtg aagctgtagg gcaggggaga
      841 gatggccatc ttcaccactt gagaacaaac cgacccggaa cggtgagaga aggccaaccc
      901 tcgatgcagt atatactaat atatactata gtctgttgat tttatgagca ttttaaaccc
      961 atagacgcca gcaacagcag aaaagcaagt tttctactgt tctgtcgccg ccgatcaacc
     1021 ccatcccttt gcatctggtg aaatctgact accaagaggt cacgaacttt gtcagatttc
     1081 acgtagcagc agcatttttt acgcaaagct acctattacg cacagctacc catcaagaca
     1141 atatattatt taccctattt tttatttgca ttttatttgt tgtgcagggt atttatttat
     1201 gtagccgcta tattgacaaa ctttttagat cggttattta tttaaatata ggttgagttc
     1261 agctttattt ttgtttttcg gaaactgagg caaggtgtaa tcccgtagcc ggtattatac
     1321 ccaatccttc ggattgccct atctcgcttt gctcgtaggg ttgaaaaaaa gatctaccct
     1381 tccgcctatt ccccttatca aaggggaggc ggtaaaccat agttttacat tgaatgttat
     1441 aaatatatat aggagtgttt aaaatgaatg agaatgatat agatcagtcc ttactagaac
     1501 tattagcaag atatttttat tatatagacc caaatgaaga aaatacagca tttcttgaag
     1561 caaataaaga tgaacaagat ttatgttatt ttgtagctta tcgctatatt aaagaaaata
     1621 aaactcaaga tcttattgat gctttgaaag atcaaaaaga tgaagattat atcaaagcaa
     1681 tgaaagatta tgtttatgga ggtggtaaat atggatacag atagaaaaag agggcgtata
     1741 ggattaaatc acgaagtacg tagagatata tctatacagt ttagagtaaa caaagcagag
     1801 tttgattatt taaaagagct tgtggaagca tcagggcttg aaagaggtca ttacatccga
     1861 aaggagattt taaactctga acccgttata aaaaggaaaa tgcctgagtt tgagcaatca
     1921 ttatacagag aaatacaaca tcaaggtaat aatataaatc agattgctaa aaagctaaat
     1981 acgaatgaag actttaaaca atctttagcc gcagaagcta actttgccat aaagcaactt
     2041 atgaaaagaa tattaaaagc ttgcgaggtt tatacagtat gaaaggaagc gtagctaaga
     2101 cgggtaagtc ttttaaaaat agagttgatt atatattaaa ggatgatcac agttttattt
     2161 gtagcaatat ggcaagtgat tataataatg ttagtgattt aactgatgag tttaaaactg
     2221 taagcagctt tagacaagat ataaaaaaac cagtctttca tgcgttttta tctcttccaa
     2281 aaaatgaaca tttaaccgat cagcaatggg aagagatagc aaaagactat ttgaaagaaa
     2341 tgaatattga tatagaaaag catcaatata tttgtgttcg tcataacgat acagataaag
     2401 aacatatcca tattgtagct aatcgtgttg gccttgatgg ttctgtatgg ctcggtcagc
     2461 actcagcatt taacacaata gcagcttgtg aacgtttaga agtaaaacac ggcttaacta
     2521 ttacacaaag tcttaaagga caaaaatcag acgtatcagc accaaccaag aacgagatcg
     2581 agcaagcctt gaggacaggt gagaaaccag cccgtatcgt gctacagaac gcattgcagg
     2641 ctgctatggt aggaaaacca gatctgcaga cgtttataga cagagtgcag gccgtaggca
     2701 ttgaaccgag gttcaatgtt gccagcactg gcaacgtggc gggggtttca ttcagtgttg
     2761 gagatgtggc attcaaggga tctcagttgg gtaaaaaata cagttggaac actataaaaa
     2821 acaaggtgat atatgacaaa aatagagatg atgaactcgt taggagcttc tcagcaagaa
     2881 aagatgatga accaaatagt attagacgat cacttgctga accaaatatc aatgacagtg
     2941 gagccgttga cagaatcgat gatgatatta tccgaatcag tcagcacagc attaaatcag
     3001 acacaacaga cacttacgag agtaaacaac tcgttagaag caacaaccac agctttaaac
     3061 tcagcgaaaa atataataga ccagcaagcc ataaagttag aagaacaaca caaaaagctg
     3121 cacaaatact caacagagtt aaaaaacaat acaataaaga accaatcaaa acttataata
     3181 aaaatggcct caatagttgg cgtgataaat ctgatagcag ttctatcagt aggatacttg
     3241 atcatgcaga aacagccaac gcagccacga ataactttag actcaaaggc cgtggcaaca
     3301 tcaatagtag agcaactaag atcgaagaga taagagaaag gttaggtata aaaaaaccta
     3361 ttattcagcc aaagaataaa gaaaaaatag ttaaagaaga aataaagaaa aagcctttag
     3421 gttggagtat gaatgaaaat agatttaaat cgaagttaaa agaagctctt aaaaaagaaa
     3481 aaccaaaaaa tgaaacaaca ccaacacaac tacagcagga agacgatcag catttaaaac
     3541 cacgcggttt taaaccaaga taaaagaggc tttaagcctc ttttgttgtt ggtgcgtcta
     3601 tttgttgata acgtttataa agagctatag ccccgtgttt tgtagttccc agcctttccc
     3661 caacctctga ccatttaaag ccagcttcac gtaatacaaa ggcttttaat cttctttgtt
     3721 ttgcttcttt ctcatagtct tcacgaggta aagcaatcaa ctttctgacg gttctatcac
     3781 tgatatttaa tctttgagcc atttcttttg ctgttttatt cctttttact gtatgtaatg
     3841 ccgtcatatt ttactcctcg ttaatcatta ttgctttttc taaaagtgtt tttgtatgta
     3901 gcttttgttt ttctgttctt ttagatcttc ttgctttctg ttttttactc tgaatatcat
     3961 gaagtttttc gttgctaaac tctttccaac aaaacttagc tatacttttt gccgttgata
     4021 caagttcttt gtaaggtaat agattattat agttagcaaa gctattgaat gtttcgcatt
     4081 tcaataataa ctctttataa aaagcatcga atgagctttc attcttatat tttagcacat
     4141 ttttataagc ccattgcctt tgagtatcga acatctcaac attacgacca aaagcaagtt
     4201 ctgtattttc tcgcttttta gggtcttcta aatcatcaaa acaatcagct aaatcattaa
     4261 gttcgtataa atcagcacca gaccagaacg gcgaccaatg gctatgcagt gggtttttag
     4321 taatcagtcc cgcatagcca agatcagcac caagacgagt agccatattg taatataatg
     4381 ctctggcaaa cttgacaggc ttctgtctgc ttttct
//
