LOCUS       NZ_CP085770             4921 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 49589CZ plasmid p49589CZ_3, complete
            sequence.
ACCESSION   NZ_CP085770
VERSION     NZ_CP085770.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377414
            Assembly: GCF_020695685.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 468x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695685.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:00:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,958
            Genes (coding)                    :: 4,822
            CDSs (with protein)               :: 4,822
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 58 of 136
            Pseudo Genes (incomplete)         :: 82 of 136
            Pseudo Genes (internal stop)      :: 26 of 136
            Pseudo Genes (multiple problems)  :: 26 of 136
            Pseudo Genes (short protein)      :: 1 of 136
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085770.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="49589CZ"
                     /isolation_source="Decubitus swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p49589CZ_3"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2019-03"
                     /collected_by="Fakultni nemocnice Kralovske Vinohrady"
     gene            complement(27..350)
                     /locus_tag="LJU37_RS22740"
                     /old_locus_tag="LJU37_22740"
     CDS             complement(27..350)
                     /locus_tag="LJU37_RS22740"
                     /old_locus_tag="LJU37_22740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_021243036.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARTGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            complement(1357..2643)
                     /locus_tag="LJU37_RS22745"
                     /old_locus_tag="LJU37_22745"
     CDS             complement(1357..2643)
                     /locus_tag="LJU37_RS22745"
                     /old_locus_tag="LJU37_22745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002437803.1"
                     /GO_process="GO:0006935 - chemotaxis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting chemotaxis
                     protein"
                     /protein_id="WP_306170312.1"
                     /translation="MFSLNLQRSAISYDAICQNKPVIEFSPEGVINKASPLFLSTMGYR
                     ADEIIGHHHRIFCPPSLVSSPEYSQFWQRLARGESFSGKYLRLAKGNRSVWLEASYIPV
                     SDRRGRVIRVIKIAADISERVHSALEQEAVVNAINRSMAIITFNPEGIVLEANENFVNA
                     TGYKRDEIIGKHHRLFCAETLYKSDEYRHFWESLNQGEFFSGLFPRLNRQGDPLWFRAT
                     YNPVFNSDGQLYKIVKFATDVTADVLRNQREQEAAVHAWDMAVQTRESAQNGANVIENS
                     ILMIDRIAQGMGAVSTDISRLNNQSESIDDMVETIRKFAMQTRLIALNAAIEAARAGAS
                     GRSFAVVAAEVRNLAASVSSATEEIEQVVASNSQLAKDVLCGIENSLMNTREGVTLMRE
                     AGEVMTSIQRNAAGVETAVKDVAISVKAG"
                     /name="PAS domain-containing methyl-accepting chemotaxis
                     protein"
     gene            complement(3557..3772)
                     /locus_tag="LJU37_RS22750"
                     /old_locus_tag="LJU37_22750"
     CDS             complement(3557..3772)
                     /locus_tag="LJU37_RS22750"
                     /old_locus_tag="LJU37_22750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_021243037.1"
                     /translation="MTELEKQLLSVLEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(3779..4270)
                     /locus_tag="LJU37_RS22755"
                     /old_locus_tag="LJU37_22755"
     CDS             complement(3779..4270)
                     /locus_tag="LJU37_RS22755"
                     /old_locus_tag="LJU37_22755"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_047720453.1"
                     /translation="MSSLLTLAKDLEQQSKAQQKSTGEMLKAAFSEHEQSVRAELNASA
                     RRISDAISAHEQSMSEAMEKNRRSVLLTAGRTWLTILMVSALLIATSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQAEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     Typing          complement(464..636)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.851"
                     /coverage="100.0"
                     /evalue="1.98e-82"
     Typing          561..1107
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.727"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          complement(31..350)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.458"
                     /coverage="100.0"
                     /evalue="5.84e-62"
     Typing          complement(3460..4919)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.041"
                     /coverage="97.0"
                     /evalue="0.0"
ORIGIN
        1 caccgccgcg gccacgtgga tgaaatttaa cgatcatcgt ccgttccttt ctcaagaacg
       61 gcgtgccgca gccgctcaag cccggcatcg atggccatca gcgccgccac aatctgcacc
      121 ctgtcatggc cggtgccgcc gccggcgtta acacggcggg ctatctggtt caggttattc
      181 cccatgccgg caagctggcg cagcagcgcc ggcgagattg aggggagctt tccggtgcgc
      241 gcaggttttt cgtccaggca ggtctgccgc atccacgccg ccagctgcct gccgtcacag
      301 cgctcaagca gccgccggtg ctcgtcctcc gtcacccaca tggtgagcat cttgttccgt
      361 ttctctgaca aaccggcctc ctgataacgc cgggacgggc gggaaagcct ttcccggtcg
      421 gcgcggggtt ggacaagccg caggcgcgtc aggcttttgg cgggtttcgg ggcgcagccc
      481 tgaaccagtc acgtagcgct agcggagtgt atactggctt actatattgg cacggatgcg
      541 aatatcagtg aagtgcttca tgtggcagga gaaaaatgca gcagcggagc gtcagcagaa
      601 tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
      661 gctgcggcga gcggtaccgg cttacaggcg gggcggaaat ttcctgggag atgccagaaa
      721 gaagcttaac agggaagcga taaggccacg gcgaagccgt ttttccatag gctccgcccc
      781 cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc gacaggacta
      841 taaagatacc aggcgtttcc ccctggtggc tccctcgtgc gctctcctgt tcctgccttt
      901 cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact
      961 cagttccggg aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc
     1021 cgaccgctgc gccttatccg gtaactatca acttgagtcc aatccggaaa aacacgacaa
     1081 agcgccactg gcagcagcca ctggtaacag gtttagagga ggagagtctt gaagtttcgg
     1141 gtgcgataag gctacactga aaggacagtt ttggtgactg tgctcctcca agccagttac
     1201 cgcggttcaa aagagcgtca actcttgcga accttcgaaa aagccacctt gccgggtggt
     1261 tttttcgttt tcagagcaag agattacgac gcaacggaaa ggatctcaag aagatcatct
     1321 gtgatgttcg acagatatgg ggatatgcac catggcctat ccggctttaa ctgaaatggc
     1381 gacatcctta accgcagtct caacccctgc agcattcctc tgaatgctgg tcataacttc
     1441 acccgcttcg cgcatgagtg taaccccttc ccgagtgttc atcaggctgt tttcaatgcc
     1501 acagagaaca tccttggcca gttgactatt gctggccacg acttgctcaa tttcttcagt
     1561 cgcgctactg acgctcgccg caagattacg cacttctgcg gcaacaaccg caaaacttct
     1621 tccggatgcg ccagcccttg ctgcttcaat cgcggcattc agtgcaataa gtcttgtctg
     1681 catggcaaac ttccggatgg tttccaccat atcgtcaata ctttcagact gattgttgag
     1741 ccgtgagata tcggtggaga cagcccccat cccctgcgca attctgtcaa tcataaggat
     1801 gctattttca ataacattgg caccgttctg cgcactttct cgggtctgca cggccatatc
     1861 ccatgcatgc acagcagcct cttgctctct ctggtttcgc aggacatcag ccgtaacatc
     1921 tgtagcgaat ttcacaattt tataaagctg cccatcactg ttaaagacag ggttataagt
     1981 tgcacggaac cacagagggt ctccctgacg gttaaggcgt ggaaacagac cagaaaaaaa
     2041 ctcaccttga ttcagactct cccagaaatg tcggtattca tcacttttgt aaagcgtctc
     2101 ggcacagaac agacgatgat gcttacctat gatttcatcc cgcttatagc cagtggcatt
     2161 gacgaaattt tcatttgctt caagcacgat cccttcagga ttgaaggtga tgatggccat
     2221 agaacggttt atggcattta caactgcttc ctgctcaaga gcagaatgca cgcgctcaga
     2281 aatatcggca gcaattttaa tgactctgat aacacggccg cgcctgtcag aaacggggat
     2341 ataactggct tcaagccata cggaacgatt tccctttgcc agccgcaggt acttgccact
     2401 aaagctttct cccctggcaa ggcgctgcca gaactggctg tattccggtg aacttaccag
     2461 cgagggcggg cagaatatac gatggtggtg accgattatt tcatcagccc tgtaccccat
     2521 agtggagaga aaaagcgggc tggctttatt tatcaccccc tccggactga actcaatgac
     2581 aggcttattc tgacaaattg cgtcatacga aatggctgat cgctggaggt tcagggagaa
     2641 catattttta agtgaaaaca tatgaatcag attgcccgga taatgtaagt actgccatgg
     2701 ttatcggcta ctccttatgt agcgttatat attactgcga cagggcggga aaaagcccgc
     2761 tattcagcca tgccggcatg atgtccggct ccggatttca cgcgtaaaaa acccgccatc
     2821 tttagcgggc tgaacaataa gaaaatgttc atatttacgg caaatccgcc ggctccagca
     2881 gagaagctgg cgtctatcaa tcttggaaga accagcgcag ctccgcatgc attaacaggc
     2941 atcagaaggc aaaaaaatgg ctcccatccg ggagccggaa aacactcaac tgcctggatc
     3001 gcagcacatc ctttgtgaag gataatttaa gtatcggccg gattgccgcg ggcataattt
     3061 tttccgtatc cgccagacct cggcttttaa cataacaccc cttgcccgca ccgcagaaac
     3121 ggggttggct gcgtttccgg tgccagacgc gagaaagtgg cgacagacat gacgaaaaaa
     3181 caccaaaaac gcagccgctg gatattacac tgatgcatag tcatgcagcc agatgcatgc
     3241 agcgtaaagt cacactaaaa tcagcgcgaa cgccttaaaa accggcaaat gcctctccgg
     3301 caactcacac agctccatca ccaggtaaaa acagttcagc agggcttcaa taacgttgag
     3361 aattttcatc acttcctccg ggataagtca ctaccattcc ggtagtcagc ctatttagca
     3421 ggaacgcgat gagcccttct tcgtgcgggc tgtcacctca tcttcggacc atcgaaactc
     3481 ttctgcttct gtcggagcgt cagctccagc tcgttctgac gctgatactc cagtgcagcc
     3541 ttctcctcga tggcattcag ttcccggaca agcggcgcag ctgctcgctt aaactcagca
     3601 cctgcgtgct caagtccgac acgcgctcgt tcagcaccgc gttctcccgt tgcataagac
     3661 cacacatgct ccgccattcc gcgaaggcgc tctcccattc gtccagcctt tgcgagtagt
     3721 cctgctgtag ctgctctaat acgctcagca actgcttttc cagctctgtc attttccgct
     3781 actcctgttt caggcggatc catgtcgtcc cgtcgtaggg gatggcttcg gcttgcatcc
     3841 ccggcggcag gataagaaac ctccggccgt cgctgctctc ctgatagcgc actccccacg
     3901 tccgggcggt cattttcgcc agggtatctt cctgctgacg aagatgcgtg taattgtcgg
     3961 ttatctgctg cccctgccac cacagaatgc tcccgctcgt ggcgatcagc agcgctgaca
     4021 ccatcaggat ggtgagccat gtcctcccgg ccgtcagcaa cacgctccgc cggtttttct
     4081 ccatcgcctc gctcatactc tgctcgtggg cgctgatggc gtcgctgatt ctcctcgcgc
     4141 ttgcgttcag ttccgctctg acagactgct cgtgctcgct gaatgcggct ttcagcatct
     4201 cgccggtact cttctgctgc gctttcgatt gctgctctaa gtccttcgcc agcgttaaaa
     4261 gactgctcat agatggctcc cttcagtcgg atgtttcgcc ccccgtcagg gtcggcaatg
     4321 ctgatactgc ttttcgttgt acgcaccacc tcaaaacctg caccttcaag agcctcagtg
     4381 acgtcctgac ggcttttcag ctccccggac gaggctaagc tcagcagtcc acgggtgatg
     4441 gcttctgcgg cttcctgctt cgttttcggc agtccggccg gcgtgaccag cgcccggcgg
     4501 ttttccggcg cgttcggatc gtgcagcccc agtctgccgt tcacgatggt ctgccaggca
     4561 tcgatacgcg gccggtcggc gcggtcgtag tacggctgga gccgcctgcc ggtcagcagc
     4621 tcggtattgg ggatcagaaa gttcagctcc agacgcccct tgtcctcgtg ctccacccac
     4681 agaatgctgt actgatcttt atcaagtccg ggcatcagaa cccgctcgaa gctggccatc
     4741 agtttttccc gctggccggg aggcagatcg gcctccgcaa acgacaggac gccggaggtg
     4801 tatttcttca cgtaaggcga cgcgtcaatc agctcccgga cttcttccgg cttcccctgc
     4861 aggacgctgg cgccttcacg ctgccggtct ttacccagca gatagtccac cggcccggcg
     4921 c
//
