LOCUS       NZ_CP085769             2811 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 49589CZ plasmid p49589CZ_2, complete
            sequence.
ACCESSION   NZ_CP085769
VERSION     NZ_CP085769.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377414
            Assembly: GCF_020695685.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 468x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695685.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:00:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,958
            Genes (coding)                    :: 4,822
            CDSs (with protein)               :: 4,822
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 58 of 136
            Pseudo Genes (incomplete)         :: 82 of 136
            Pseudo Genes (internal stop)      :: 26 of 136
            Pseudo Genes (multiple problems)  :: 26 of 136
            Pseudo Genes (short protein)      :: 1 of 136
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085769.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="49589CZ"
                     /isolation_source="Decubitus swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p49589CZ_2"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2019-03"
                     /collected_by="Fakultni nemocnice Kralovske Vinohrady"
     gene            complement(175..774)
                     /locus_tag="LJU37_RS22725"
                     /old_locus_tag="LJU37_22725"
     CDS             complement(175..774)
                     /locus_tag="LJU37_RS22725"
                     /old_locus_tag="LJU37_22725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_001029633.1"
                     /translation="MAKAKVLINDTDEAVIKNALDVEDMNEVNINVTRKKKTGFLGTYS
                     IVFHDSVKTLSKMNLKPNAYKIVMYLFGIIEMGNILVNFSQKKIADDLGLQPSNVSRAF
                     KELFDKGVLIKDSEDGHVYLNSNLCTMGVPKNFDQKRMKSLQKSNIETEDFKNQINLFT
                     PTNTAKIKTNDKKVIKKKEKENVLEFPMQNDWVDIE"
                     /name="Crp/Fnr family transcriptional regulator"
     gene            complement(1459..2742)
                     /locus_tag="LJU37_RS22730"
                     /old_locus_tag="LJU37_22730"
     CDS             complement(1459..2742)
                     /locus_tag="LJU37_RS22730"
                     /old_locus_tag="LJU37_22730"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_227524674.1"
                     /translation="MKSQFMHVELYSREEPAKKTINKSKNINHKSDVRTTTVNGVLSEM
                     KREEGFTSHLETIAAPEVLYGSIDALERSIERYEAEFKTTDKNGKEKKLRKDACILLAG
                     VVSLDRADEEIWDEYKTKAIEYLKNKYGENLKCVVEHTDETNPHFHFYVVGNKDQDLNL
                     LHDGKLAVSKLAKEDKKKLHQTAYTEAMIKFQDDFYVQVSNKFGLSRTGEEPRERYKSR
                     PDYLRFKKRQETALNILANLEEFEEKELKKAKQAGFNVGIKNFDETNFFRKLDWRVKRI
                     KKEKDNEIKKLSDENDNLNIDKSNLQHELDMSKIELSDAYSQRNEASRKNSVYIDKVNA
                     LKKENKLLLPYKDQFDLKLKQEKEPLQAEVKELTSENTNLKNENTKLKKENLDFKEFIL
                     GVKNYYGSKFQEWYEEVFNPKTKTKPKF"
                     /name="plasmid recombination protein"
ORIGIN
        1 tataggaacc gactatatct attacgctag catatttcat atgctgctcc atagatgcgg
       61 ccctacgggg ttaaagtcaa cttcaaaaca aaaaagcaaa tccctaaagc ttgcaaattt
      121 tatatttgcg aggtttaggg attcagcaaa cgaagtgcgg tagtgttagt tttactattc
      181 aatatcaacc caatcatttt gcattggaaa ctctaaaacg ttttcttttt cttttttctt
      241 gataactttt ttatcattag tttttatttt tgcagtgttt gttggagtaa ataaattaat
      301 ctgattttta aaatcttcag tttcaatatt tgatttctgt aagcttttca ttctcttttg
      361 atcaaaattt ttcggaacac ccatagtgca tagatttgaa tttaagtaaa cgtgtccgtc
      421 ttcgctatct ttgattaaaa cccctttatc aaaaagctct ttgaaagctc ttgatacatt
      481 agacggctgc aagcctaaat catcagctat cttcttttgt gaaaagttaa ctaaaatatt
      541 acccatctct ataatgccga ataagtacat tacaatctta taagcatttg gctttaagtt
      601 cattttagat agtgttttca cagagtcatg aaatactatt gaataagtcc ctaaaaatcc
      661 ggttttcttt tttctcgtga cgttaatatt tacttcgttc atatcttcaa catctaatgc
      721 gttttttatt actgcttcat ctgtatcgtt tattaaaact tttgcttttg ccattttata
      781 ttcctttttc taataaaaat aataacctat gttgcattta taaccgatct aaatgcaatg
      841 tcaatatcaa aacgataaga aagttatcgt ttcaacatca aatcatatca atgcacctgt
      901 atgcaaaatt atgattttca taagtctgat tatgattttc ataattttgc attatgattt
      961 tcataatcga aaattatgat tttcataatt ttttttttgg taaagtgttg atatataagg
     1021 ctttaaagtg tgcttaagaa gtaagaaaat aagaaagaag aaaccgtaaa atcaactgca
     1081 aaaccgccct cgccgggcag gccaacccct cgaaccacag aaccacaagt ggttctgcag
     1141 ttctcacggt tcaaagttta acagtatttc tttgttttat atgtagttac ataccaaagg
     1201 gggaaccccg ccccgttccc cctcttcaca acgcaccaag gcgttatgaa ggtaccccca
     1261 ccccaatgcg gccagctccc tgaccacatt ttcccaacct gcattttttg tatttaagtg
     1321 actgatattt aaaggtaact aactgcgtgc aaaaccactt tttttgggat aaatcccagc
     1381 tatgacagct aaacgctgtc aggccgcttc tggtggtgtc tcatacacca cataggcggc
     1441 aaatatgaaa atattcaatt aaaatttggg ttttgttttt gtttttgggt tgaatacttc
     1501 ctcgtaccat tcctgaaatt tagatccata gtaattttta acacccaaaa taaattcttt
     1561 gaaatctaaa ttttcttttt ttaattttgt gttctcattt ttcaaattgg tattttcaga
     1621 agtcaattct ttgacttctg cttgcaatgg ctctttttcc tgctttaatt tcaaatcaaa
     1681 ttgatctttg tatggaagta ataatttatt ttcttttttt agagcgttta ctttatcaat
     1741 ataaacgcta ttttttctgg atgcttcatt cctttgacta tatgcatctg aaagctcaat
     1801 tttactcata tctaattcat gctgtaaatt tgacttatct atatttaaat tatcgttttc
     1861 atccgataat tttttaatct cattgtcttt ttcttttttt attcttttta ctctccaatc
     1921 gagtttccta aaaaaattag tttcatcgaa atttttaata ccaacattaa agccagcttg
     1981 ttttgctttt tttaattctt tttcttcaaa ctcttctaaa ttggctaata tatttaatgc
     2041 tgtttcttgt ctttttttaa atctcaagta atcagggcgt gatttatagc gttctcgcgg
     2101 ctcctcgccc gttctgctca atccaaattt atttgatacc tgtacataaa aatcatcttg
     2161 aaatttgatc atagcttctg tgtaagctgt ttgatgtaat tttttcttat cttcttttgc
     2221 taatttagat actgcgagct ttccatcatg aagcaaattt aaatcttgat ccttgtttcc
     2281 aacgacgtaa aaatgaaaat gtgggttggt ttcgtctgta tgctcaacaa cacattttaa
     2341 attttcacca tatttatttt ttaaatattc aattgctttg gtcttatatt catcccaaat
     2401 ttcttcatca gctcgatcta gagaaaccac accagctagc aagatgcatg catctttgcg
     2461 tagcttcttc tccttcccgt tcttgtctgt agtcttaaat tcggcctcgt aacgctctat
     2521 agagcgttct aaggcatcta tagacccata gagcacctcc ggtgcagcta tggtttctaa
     2581 atgggatgta aaaccctcct cccttttcat ttctgacaac acaccgttta cggttgttgt
     2641 tctaacatca gatttatgat ttatattttt acttttattt attgtctttt tagctggttc
     2701 ttctctgctg tagagttcta catgcataaa ttgtgatttc attttaaatt ctcctatatt
     2761 ttaattttcc ttatatatgt aattaacatt tattgttttt ctgtcaatag c
//
